Package: openPrimeR 1.29.0
Matthias Döring
openPrimeR: Multiplex PCR Primer Design and Analysis
An implementation of methods for designing, evaluating, and comparing primer sets for multiplex PCR. Primers are designed by solving a set cover problem such that the number of covered template sequences is maximized with the smallest possible set of primers. To guarantee that high-quality primers are generated, only primers fulfilling constraints on their physicochemical properties are selected. A Shiny app providing a user interface for the functionalities of this package is provided by the 'openPrimeRui' package.
Authors:
openPrimeR_1.29.0.tar.gz
openPrimeR_1.29.0.zip(r-4.5)openPrimeR_1.29.0.zip(r-4.4)openPrimeR_1.29.0.zip(r-4.3)
openPrimeR_1.29.0.tgz(r-4.4-any)openPrimeR_1.29.0.tgz(r-4.3-any)
openPrimeR_1.29.0.tar.gz(r-4.5-noble)openPrimeR_1.29.0.tar.gz(r-4.4-noble)
openPrimeR_1.29.0.tgz(r-4.4-emscripten)openPrimeR_1.29.0.tgz(r-4.3-emscripten)
openPrimeR.pdf |openPrimeR.html✨
openPrimeR/json (API)
NEWS
| # Install 'openPrimeR' in R: |
| install.packages('openPrimeR', repos = c('https://bioc.r-universe.dev', 'https://cloud.r-project.org')) |
- feature.matrix - Data Sets.
- primer.data - Data Sets.
- primer.data - Data Sets.
- primer.df - Data Sets.
- ref.data - Data Sets.
- settings - Data Sets.
- settings - Data Sets.
- template.data - Data Sets.
- template.df - Data Sets.
- tiller.primer.df - Data Sets.
- tiller.settings - Data Sets.
- tiller.template.df - Data Sets.
On BioConductor:openPrimeR-1.29.0(bioc 3.21)openPrimeR-1.28.0(bioc 3.20)
This package does not link to any Github/Gitlab/R-forge repository. No issue tracker or development information is available.
softwaretechnologycoveragemultiplecomparison
Last updated 2 months agofrom:74182ac216. Checks:OK: 1 WARNING: 6. Indexed: yes.
| Target | Result | Date |
|---|---|---|
| Doc / Vignettes | OK | Dec 29 2024 |
| R-4.5-win | WARNING | Nov 29 2024 |
| R-4.5-linux | WARNING | Dec 29 2024 |
| R-4.4-win | WARNING | Nov 29 2024 |
| R-4.4-mac | WARNING | Dec 29 2024 |
| R-4.3-win | WARNING | Nov 29 2024 |
| R-4.3-mac | WARNING | Dec 29 2024 |
Exports:adjust_binding_regionsassign_binding_regionscbind2check_constraintscheck_restriction_sitesclassify_design_problemconOptionsconOptions<-constraintLimitsconstraintLimits<-ConstraintOptionsconstraintsconstraints<-ConstraintSettingsCoverageConstraintscreate_coverage_xlscreate_reportcvg_constraintscvg_constraints<-design_primersfilter_primersget_comparison_tableget_cvg_ratioget_cvg_statsget_cvg_stats_primerget_initial_primersparallel_setupPCRPCR_ConditionsPCR<-plot_conservationplot_constraintplot_constraint_deviationplot_constraint_fulfillmentplot_cvg_constraintsplot_cvg_vs_set_sizeplot_penalty_vs_set_sizeplot_primerplot_primer_binding_regionsplot_primer_cvgplot_primer_subsetsplot_template_cvgprimer_significancePrimersrbind2read_primersread_settingsread_templatesrunTutorialscore_conservationscore_degenscore_primersselect_regions_by_conservationsubset_primer_setTemplatesupdate_template_cvgwrite_primerswrite_settingswrite_templates
Dependencies:ade4apeaskpassbackportsbase64encBiocGenericsBiostringsbslibcachemcheckmatecliclustercodetoolscolorspacecrayoncurldata.tableDBIDECIPHERdigestdplyrevaluatefansifarverfastmapfontawesomeforeachforeignFormulafsgenericsGenomeInfoDbGenomeInfoDbDataGenomicRangesggplot2gluegridExtragtablehighrHmischtmlTablehtmltoolshtmlwidgetshttrIRangesisobanditeratorsjquerylibjsonliteknitrlabelinglatticelifecyclelpSolveAPImagrittrMASSMatrixmemoisemgcvmimemunsellnlmennetopensslopenxlsxpillarpixmappkgconfigplyrpwalignR6rappdirsRColorBrewerRcppRcppArmadilloreshape2rlangrmarkdownrpartrstudioapiS4VectorssassscalessegmentedseqinrspstringdiststringistringrsystibbletidyselecttinytexUCSC.utilsuniqtagutf8vctrsviridisviridisLitewithrxfunXMLXVectoryamlzip
Readme and manuals
Help Manual
| Help page | Topics |
|---|---|
| Multiplex PCR Primer Design and Analysis. | openPrimeR-package openPrimeR |
| Primer Analysis Statistics. | AnalysisStats get_comparison_table get_cvg_ratio get_cvg_stats get_cvg_stats_primer |
| Data Sets. | Data feature.matrix primer.data primer.df ref.data RefCoverage settings template.data template.df Tiller tiller.primer.df tiller.settings tiller.template.df |
| Input Functionalities. | Input Primers Primers-class read_primers read_settings read_templates Templates Templates-class |
| Output Functionalities. | create_coverage_xls create_report Output write_primers write_settings write_templates |
| Plotting Functions. | Plots plot_conservation plot_constraint plot_constraint_deviation plot_constraint_fulfillment plot_cvg_constraints plot_cvg_vs_set_size plot_penalty_vs_set_size plot_primer plot_primer_binding_regions plot_primer_cvg plot_primer_subsets plot_template_cvg |
| Primer Design Functionalities. | classify_design_problem design_primers get_initial_primers PrimerDesign |
| Primer Evaluation. | check_constraints check_restriction_sites filter_primers PrimerEval primer_significance subset_primer_set |
| The openPrimeR Tutorial. | runTutorial |
| Scoring Functions. | score_conservation score_degen score_primers Scoring |
| Settings Functionalities. | conOptions conOptions,DesignSettings-method conOptions<- conOptions<-,DesignSettings-method constraintLimits constraintLimits,DesignSettings-method constraintLimits<- constraintLimits<-,DesignSettings-method ConstraintOptions ConstraintOptions-class constraints constraints,AbstractConstraintSettings-method constraints,DesignSettings-method constraints<- constraints<-,AbstractConstraintSettings,list-method constraints<-,DesignSettings,list-method ConstraintSettings ConstraintSettings-class CoverageConstraints CoverageConstraints-class cvg_constraints cvg_constraints,DesignSettings-method cvg_constraints<- cvg_constraints<-,DesignSettings-method DesignSettings DesignSettings-class parallel_setup PCR PCR,DesignSettings-method PCR<- PCR<-,DesignSettings-method PCR_Conditions PCR_Conditions-class Settings |
| Template Functionalities. | adjust_binding_regions assign_binding_regions select_regions_by_conservation TemplatesFunctions update_template_cvg |
