Package: MassArray 1.59.0

Reid F. Thompson

MassArray: Analytical Tools for MassArray Data

This package is designed for the import, quality control, analysis, and visualization of methylation data generated using Sequenom's MassArray platform. The tools herein contain a highly detailed amplicon prediction for optimal assay design. Also included are quality control measures of data, such as primer dimer and bisulfite conversion efficiency estimation. Methylation data are calculated using the same algorithms contained in the EpiTyper software package. Additionally, automatic SNP-detection can be used to flag potentially confounded data from specific CG sites. Visualization includes barplots of methylation data as well as UCSC Genome Browser-compatible BED tracks. Multiple assays can be positionally combined for integrated analysis.

Authors:Reid F. Thompson <[email protected]>, John M. Greally <[email protected]>

MassArray_1.59.0.tar.gz
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MassArray.pdf |MassArray.html
MassArray/json (API)

# Install 'MassArray' in R:
install.packages('MassArray', repos = c('https://bioc.r-universe.dev', 'https://cloud.r-project.org'))

Peer review:

Datasets:

On BioConductor:MassArray-1.57.0(bioc 3.20)MassArray-1.56.0(bioc 3.19)

This package does not link to any Github/Gitlab/R-forge repository. No issue tracker or development information is available.

immunooncologydnamethylationsnpmassspectrometrygeneticsdataimportvisualization

4.30 score 1 scripts 190 downloads 84 mentions 31 exports 0 dependencies

Last updated 23 days agofrom:53960bca4d. Checks:OK: 1 NOTE: 6. Indexed: yes.

TargetResultDate
Doc / VignettesOKOct 30 2024
R-4.5-winNOTEOct 30 2024
R-4.5-linuxNOTEOct 30 2024
R-4.4-winNOTEOct 30 2024
R-4.4-macNOTEOct 30 2024
R-4.3-winNOTEOct 30 2024
R-4.3-macNOTEOct 30 2024

Exports:ampliconPredictionanalyzeCpGsbisConvertcalcMethcalcMWcalcPercentAdductcalcPercentConversioncombineconvControlcountCGscreateWiggleestimatePrimerDimerevaluateSNPsexpandSequencefindCollisionsfindFragmentsfindPeaksidentifySNPsimportEpiTyperDataimportEpiTyperData.newinSilicoFragmentationisAssayablenumCollisionsplot.MassArrayDatapositionposition<-revComplementrnaDigestsamplessamples<-sum.MassArraySpectrum

Dependencies:

Primer

Rendered fromMassArray.Rnwusingutils::Sweaveon Oct 30 2024.

Last update: 2019-12-23
Started: 2013-11-01

Readme and manuals

Help Manual

Help pageTopics
Amplicon predictionampliconPrediction
Analyze CG methylationanalyzeCpGs
Bisulphite conversionbisConvert
Calculate percent methylationcalcMeth
Calculate molecular weightcalcMW
Calculate percent salt adductscalcPercentAdduct
Calculate percent conversioncalcPercentConversion
Combine MassArrayData objectscombine
Combine MassArrayData objects (methods)combine,MassArrayData,MassArrayData-method combine,MassArrayData,missing-method combine-methods
Conversion controlconvControl
Count number of CGscountCGs
Create wiggle trackcreateWiggle
Estimate Primer DimersestimatePrimerDimer
Evaluate SNPsevaluateSNPs
Expand nucleotide sequenceexpandSequence
Find peak collisionsfindCollisions
Find fragmentsfindFragments
Find peaksfindPeaks
Identify SNPsidentifySNPs
Import EpiTyper data (v.1.0)importEpiTyperData
Import EpiTyper data (v.1.0.5)importEpiTyperData.new
In silico fragmentationinSilicoFragmentation
Is assayable?isAssayable
MassArray Data objectMassArray.example.data
Class "MassArrayData"$,MassArrayData-method $<-,MassArrayData-method initialize,MassArrayData-method MassArrayData-class [,MassArrayData-method
Class "MassArrayFragment"$,MassArrayFragment-method $<-,MassArrayFragment-method initialize,MassArrayFragment-method MassArrayFragment-class
Class "MassArrayPeak"$,MassArrayPeak-method $<-,MassArrayPeak-method initialize,MassArrayPeak-method MassArrayPeak-class
Class "MassArraySpectrum"$,MassArraySpectrum-method $<-,MassArraySpectrum-method initialize,MassArraySpectrum-method MassArraySpectrum-class
Count peak collisionsnumCollisions
Plot MassArrayDataplot.MassArrayData
Operate on positional informationposition position<-
Operate on positional information (methods)position,MassArrayData-method position-methods position<-,MassArrayData,character-method position<-,MassArrayData,missing-method
Reverse complementrevComplement
Reverse complement (methods)revComplement,character-method revComplement,MassArrayData-method revComplement-methods
RNA digestrnaDigest
Operate on sample name(s)samples samples<-
Operate on sample name(s) (methods)samples,MassArrayData-method samples-methods samples<-,MassArrayData,character-method samples<-,MassArrayData,missing-method
Sum MassArraySpectrum objectssum.MassArraySpectrum