Package: EventPointer 3.15.0

Juan A. Ferrer-Bonsoms

EventPointer: An effective identification of alternative splicing events using junction arrays and RNA-Seq data

EventPointer is an R package to identify alternative splicing events that involve either simple (case-control experiment) or complex experimental designs such as time course experiments and studies including paired-samples. The algorithm can be used to analyze data from either junction arrays (Affymetrix Arrays) or sequencing data (RNA-Seq). The software returns a data.frame with the detected alternative splicing events: gene name, type of event (cassette, alternative 3',...,etc), genomic position, statistical significance and increment of the percent spliced in (Delta PSI) for all the events. The algorithm can generate a series of files to visualize the detected alternative splicing events in IGV. This eases the interpretation of results and the design of primers for standard PCR validation.

Authors:Juan Pablo Romero [aut], Juan A. Ferrer-Bonsoms [aut, cre], Pablo Sacristan [aut], Ander Muniategui [aut], Fernando Carazo [aut], Ander Aramburu [aut], Angel Rubio [aut]

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EventPointer.pdf |EventPointer.html
EventPointer/json (API)
NEWS

# Install 'EventPointer' in R:
install.packages('EventPointer', repos = c('https://bioc.r-universe.dev', 'https://cloud.r-project.org'))

Peer review:

Bug tracker:https://github.com/jpromeror/eventpointer/issues

Datasets:
  • AllEvents_RNASeq - Alternative splicing events detected by EventPointer
  • AllEvents_RNASeq_MP - Alternative splicing multi-path events detected by EventPointer
  • ArrayDatamultipath - Preprocessed arrays data with multi-path events
  • ArraysData - Preprocessed arrays data
  • EventXtrans - Relationship between isoforms and events
  • Fit - Result of EventPointer_Bootstrap
  • MyPrimers - Data frame with primers design for conventional PCR
  • MyPrimers_taqman - Data frame with primers design for taqman PCR
  • PSIss - Relationship between isoforms and events
  • SG_RNASeq - Splicing graph elements predicted from BAM files
  • SG_reclassify - Splicing graph example for Events_ReClassification function
  • TxD - Transcript x Protein Domain matrix: small matrix for examples

On BioConductor:EventPointer-3.13.0(bioc 3.20)EventPointer-3.12.0(bioc 3.19)

alternativesplicingdifferentialsplicingmrnamicroarrayrnaseqtranscriptionsequencingtimecourseimmunooncology

5.30 score 4 stars 5 scripts 250 downloads 20 exports 139 dependencies

Last updated 23 days agofrom:8a000b5f7a. Checks:OK: 1 NOTE: 8. Indexed: yes.

TargetResultDate
Doc / VignettesOKOct 30 2024
R-4.5-win-x86_64NOTEOct 30 2024
R-4.5-linux-x86_64NOTEOct 30 2024
R-4.4-win-x86_64NOTEOct 30 2024
R-4.4-mac-x86_64NOTEOct 31 2024
R-4.4-mac-aarch64NOTEOct 31 2024
R-4.3-win-x86_64NOTEOct 30 2024
R-4.3-mac-x86_64NOTEOct 31 2024
R-4.3-mac-aarch64NOTEOct 31 2024

Exports:CDFfromGTFCDFfromGTF_MultipathEventDetectionEventDetection_transcriptomeEventDetectionMultipathEventPointerEventPointer_BootstrapsEventPointer_IGVEventPointer_RNASeqEventPointer_RNASeq_IGVEventPointer_RNASeq_TranRefEventPointer_RNASeq_TranRef_IGVEvents_ReClassificationFindPrimersgetbootstrapdataGetPSI_FromTranRefPrepareBam_EPProtein_Domain_EnrichmentPSI_StatisticResulTable

Dependencies:abindaffxparserAnnotationDbiaskpassBHbiglmBiobaseBiocGenericsBiocIOBiocParallelBiostringsbitbit64bitopsblobBSgenomecachemclicobscodetoolscolorspacecowplotcpp11crayoncurldata.tableDBIDelayedArraydiagramdigestdoParallelfansifarverfastmapfastmatchfgseaforeachformatRfutile.loggerfutile.optionsfuturefuture.applyGenomeInfoDbGenomeInfoDbDataGenomicAlignmentsGenomicFeaturesGenomicRangesggplot2glmnetglobalsgluegraphgtablehttrigraphIRangesisobanditeratorsjsonliteKEGGRESTKernSmoothlabelinglambda.rlatticelavalifecyclelimmalistenvlpSolvemagrittrMASSMatrixMatrixGenericsMatrixModelsmatrixStatsmemoisemgcvmimemunsellnlmennlsnumDerivopensslparallellypillarpkgconfigplogrplyrpngpoibinprodlimprogressrquantregqvalueR6RBGLRColorBrewerRcppRcppEigenRCurlreshape2restfulrrhdf5rhdf5filtersRhdf5libRhtslibrjsonrlangRsamtoolsRSQLitertracklayerRUnitS4ArraysS4VectorsscalesSGSeqshapesnowSparseArraySparseMspeedglmSQUAREMstatmodstringistringrSummarizedExperimentsurvivalsystibbletximportUCSC.utilsutf8vctrsviridisLitewithrXMLXVectoryamlzlibbioc

EventPointer: An effective identification of alternative splicing events using junction arrays and RNA-Seq data

Rendered fromEventPointer.Rmdusingknitr::rmarkdownon Oct 30 2024.

Last update: 2023-03-15
Started: 2017-02-17

Readme and manuals

Help Manual

Help pageTopics
Alternative splicing events detected by EventPointerAllEvents_RNASeq
Alternative splicing multi-path events detected by EventPointerAllEvents_RNASeq_MP
Preprocessed arrays data with multi-path eventsArrayDatamultipath
Preprocessed arrays dataArraysData
CDF file creation for EventPointerCDFfromGTF
CDF file creation for EventPointer (MultiPath)CDFfromGTF_Multipath
Events X RBPS matrix creationCreateExSmatrix
Detect splicing events using EventPointer methodologyEventDetection
EventDetection_transcriptomeEventDetection_transcriptome
Detect splicing multipath events using EventPointer methodologyEventDetectionMultipath
EventPointerEventPointer
EventPointer_BootstrapsEventPointer_Bootstraps
EventPointer IGV VisualizationEventPointer_IGV
Statistical analysis of alternative splcing events for RNASeq dataEventPointer_RNASeq
EventPointer RNASeq IGV VisualizationEventPointer_RNASeq_IGV
EventPointer_RNASeq_TranRefEventPointer_RNASeq_TranRef
EventPointer RNASeq from reference transcriptome IGV VisualizationEventPointer_RNASeq_TranRef_IGV
Events_ReClassificationEvents_ReClassification
relationship between isoforms and eventsEventXtrans
FindPrimersFindPrimers
Result of EventPointer_BootstrapFit
getbootstrapdatagetbootstrapdata
GetPSI_FromTranRefGetPSI_FromTranRef
Data frame with primers design for conventional PCRMyPrimers
Data frame with primers design for taqman PCRMyPrimers_taqman
Bam files preparation for EventPointerPrepareBam_EP
Protein_Domain_EnrichmentProtein_Domain_Enrichment
PSI_StatisticPSI_Statistic
relationship between isoforms and eventsPSIss
ResulTableResulTable
Splicing Factor PredictionSF_Prediction
Splicing graph example for Events_ReClassification functionSG_reclassify
Splicing graph elements predicted from BAM filesSG_RNASeq
Transcript x Protein Domain matrix: small matrix for examplesTxD