Package: pram 1.23.0

Peng Liu

pram: Pooling RNA-seq datasets for assembling transcript models

Publicly available RNA-seq data is routinely used for retrospective analysis to elucidate new biology. Novel transcript discovery enabled by large collections of RNA-seq datasets has emerged as one of such analysis. To increase the power of transcript discovery from large collections of RNA-seq datasets, we developed a new R package named Pooling RNA-seq and Assembling Models (PRAM), which builds transcript models in intergenic regions from pooled RNA-seq datasets. This package includes functions for defining intergenic regions, extracting and pooling related RNA-seq alignments, predicting, selected, and evaluating transcript models.

Authors:Peng Liu [aut, cre], Colin N. Dewey [aut], Sündüz Keleş [aut]

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NEWS

# Install 'pram' in R:
install.packages('pram', repos = c('https://bioc.r-universe.dev', 'https://cloud.r-project.org'))

Peer review:

Bug tracker:https://github.com/pliu55/pram/issues

On BioConductor:pram-1.23.0(bioc 3.21)pram-1.22.0(bioc 3.20)

softwaretechnologysequencingrnaseqbiologicalquestiongenepredictiongenomeannotationresearchfieldtranscriptomicsbioconductor-packagegenome-annotationrna-seqtranscript-model

4.18 score 1 stars 3 scripts 178 downloads 6 exports 51 dependencies

Last updated 2 months agofrom:a4a7d7e0a8. Checks:OK: 7. Indexed: yes.

TargetResultDate
Doc / VignettesOKDec 03 2024
R-4.5-winOKDec 03 2024
R-4.5-linuxOKDec 03 2024
R-4.4-winOKDec 03 2024
R-4.4-macOKDec 03 2024
R-4.3-winOKDec 03 2024
R-4.3-macOKDec 03 2024

Exports:buildModeldefIgRangesevalModelprepIgBamrunPRAMselModel

Dependencies:abindaskpassBHBiobaseBiocGenericsBiocIOBiocParallelBiostringsbitopscodetoolscpp11crayoncurldata.tableDelayedArrayformatRfutile.loggerfutile.optionsgenericsGenomeInfoDbGenomeInfoDbDataGenomicAlignmentsGenomicRangeshttrIRangesjsonlitelambda.rlatticeMatrixMatrixGenericsmatrixStatsmimeopensslR6RCurlrestfulrRhtslibrjsonRsamtoolsrtracklayerS4ArraysS4VectorssnowSparseArraySummarizedExperimentsysUCSC.utilsXMLXVectoryamlzlibbioc

PRAM: Pooling RNA-seq and Assembling Models

Rendered frompram.Rmdusingknitr::rmarkdownon Dec 03 2024.

Last update: 2022-10-09
Started: 2018-05-15

Readme and manuals

Help Manual

Help pageTopics
Build transcript models from aligned RNA-seq databuildModel
Define intergenic genomic regionsdefIgRanges
Evaluate transcript modelevalModel evalModel,character,character-method evalModel,character,data.table-method evalModel,data.table,data.table-method evalModel,GRanges,GRanges-method
Extract alignments in intergenic regions from BAM filesprepIgBam
Predict intergenic transcript models from RNA-seqrunPRAM
Select transcript modelsselModel