Package: derfinder 1.47.0

Leonardo Collado-Torres

derfinder: Annotation-agnostic differential expression analysis of RNA-seq data at base-pair resolution via the DER Finder approach

This package provides functions for annotation-agnostic differential expression analysis of RNA-seq data. Two implementations of the DER Finder approach are included in this package: (1) single base-level F-statistics and (2) DER identification at the expressed regions-level. The DER Finder approach can also be used to identify differentially bounded ChIP-seq peaks.

Authors:Leonardo Collado-Torres [aut, cre], Alyssa C. Frazee [ctb], Andrew E. Jaffe [aut], Jeffrey T. Leek [aut, ths]

derfinder_1.47.0.tar.gz
derfinder_1.47.0.zip(r-4.7)derfinder_1.47.0.zip(r-4.6)derfinder_1.47.0.zip(r-4.5)
derfinder_1.47.0.tgz(r-4.6-any)derfinder_1.47.0.tgz(r-4.5-any)
derfinder_1.47.0.tar.gz(r-4.7-any)derfinder_1.47.0.tar.gz(r-4.6-any)
derfinder_1.47.0.tgz(r-4.6-emscripten)
manual.pdf |manual.html
DESCRIPTION |NEWS
card.svg |card.png
derfinder/json (API)

# Install 'derfinder' in R:
install.packages('derfinder', repos = c('https://bioc.r-universe.dev', 'https://cloud.r-project.org'))

Bug tracker:https://github.com/lcolladotor/derfinder/issues

Datasets:

On BioConductor:derfinder-1.47.0(bioc 3.24)derfinder-1.46.0(bioc 3.23)

differentialexpressionsequencingrnaseqchipseqdifferentialpeakcallingsoftwareimmunooncologycoverageannotation-agnosticbioconductorderfinder

10.19 score 45 stars 6 packages 79 scripts 6 mentions 26 exports 127 dependencies

Last updated from:ab0f068105. Checks:1 WARNING, 7 NOTE, 2 OK. Indexed: yes.

TargetResultTimeFilesSyslog
bioc-checksWARNING285
linux-devel-x86_64NOTE615
source / vignettesOK474
linux-release-x86_64NOTE628
macos-release-arm64NOTE435
macos-oldrel-arm64NOTE361
windows-develNOTE510
windows-releaseNOTE523
windows-oldrelNOTE533
wasm-releaseOK258

Exports:advancedArganalyzeChrannotateRegionscalculatePvaluescalculateStatscoerceGRcollapseFullCoveragecoverageToExoncreateBwcreateBwSampledefine_clusterextendedMapSeqlevelsfilterDatafindRegionsfullCoveragegetRegionCoveragegetTotalMappedloadCoveragemakeGenomicStatemakeModelsmergeResultspreprocessCoveragerailMatrixrawFilesregionMatrixsampleDepth

Dependencies:abindAnnotationDbiaskpassbackportsbase64encBHBiobaseBiocBaseUtilsBiocGenericsBiocIOBiocParallelBiostringsbitbit64bitopsblobBSgenomebslibbumphuntercachemcheckmatecigarillocliclustercodetoolscolorspacecpp11crayoncurldata.tableDBIDelayedArrayderfinderHelperdigestdoRNGevaluatefarverfastmapfontawesomeforeachforeignformatRFormulafsfutile.loggerfutile.optionsgenericsGenomeInfoDbGenomicAlignmentsGenomicFeaturesGenomicFilesGenomicRangesggplot2gluegridExtragtablehighrHmischtmlTablehtmltoolshtmlwidgetshttrIRangesisobanditeratorsjquerylibjsonliteKEGGRESTknitrlabelinglambda.rlatticelifecyclelimmalocfitmagrittrMatrixMatrixGenericsmatrixStatsmemoisemimennetopensslpkgconfigplyrpngqvalueR6rappdirsRColorBrewerRcppRCurlreshape2restfulrRhtslibrjsonrlangrmarkdownrngtoolsrpartRsamtoolsRSQLiterstudioapirtracklayerS4ArraysS4VectorsS7sassscalesSeqinfosnowSparseArraystatmodstringistringrSummarizedExperimentsystinytexUCSC.utilsVariantAnnotationvctrsviridisLitewithrxfunXMLXVectoryaml

derfinder quick start guide
Basics | Install r Biocpkg('derfinder') | Required knowledge | Asking for help | Citing r Biocpkg('derfinder') | Quick start to using to r Biocpkg('derfinder') | Introduction | Sample DER Finder analysis | Reproducibility | Bibliography

Last update: 2025-07-22
Started: 2015-12-09

derfinder users guide
Asking for help | r Biocpkg('derfinder') users guide | Expressed regions-level | regionMatrix() | railMatrix() | Single base-level F-statistics | Example data | Phenotype data | Load the data | Filter coverage | Expressed region-level analysis | Via regionMatrix() | Find DERs with r Biocpkg('DESeq2') | Find DERs with r Biocpkg('limma') | Via railMatrix() | Single base-level F-statistics analysis | Models | Find candidate DERs | Explore results | optionStats | coveragePrep | fstats | regions | Nearest annotation | Time spent | Merge results | optionsMerge | fullRegions | fullAnnotatedRegions | ChIP-seq differential binding | Visually explore results | Interactive HTML reports | Miscellaneous features | Feature level analysis | Compare results visually | Export coverage to BigWig files | Advanced arguments | Non-human data | Functions that use multiple cores | Loading data details | Controlling loading from BAM files | Unfiltered base-level coverage | Input files in a different naming style | Loading data in chunks | Large number of samples | Flow charts | DER analysis flow chart | analyzeChr() flow chart | regionMatrix() flow chart | Base-level F-statistics projects | File organization | bash scripts | derfinder-analysis.R | derAnalysis.sh | Expressed regions-level projects | Summary | Reproducibility | Bibliography

Last update: 2024-12-12
Started: 2015-11-11

Readme and manuals

Help Manual

Help pageTopics
Run the derfinder analysis on a chromosomeanalyzeChr
Assign genomic states to regionsannotateRegions
Calculate p-values and identify regionscalculatePvalues
Calculate F-statistics at base pair resolution from a loaded BAM filescalculateStats
Coerce the coverage to a GRanges object for a given samplecoerceGR
Collapse full coverage information for efficient quantile computationscollapseFullCoverage
Extract coverage information for exonscoverageToExon
Export coverage to BigWig filescreateBw
Create a BigWig file with the coverage information for a given samplecreateBwSample
Define a cluster to use.define_cluster
Deprecated functions in package 'derfinder'advancedArg derfinder-deprecated
Change naming style for a set of sequence namesextendedMapSeqlevels
Filter the positions of interestfilterData
Find non-zero regions in a RlefindRegions
Load the unfiltered coverage information from a group of BAM files and a list of chromosomesfullCoverage
Genome samples processed datagenomeData
Genome samples processed datagenomeDataRaw
F-statistics for the example datagenomeFstats
Genome samples informationgenomeInfo
Candidate DERs for example datagenomeRegions
Genomic State for Hsapiens.UCSC.hg19.knownGenegenomicState
Extract coverage information for a set of regionsgetRegionCoverage
Calculate the total number of mapped readsgetTotalMapped
Load the coverage information from a group of BAM filesloadCoverage
Obtain the genomic state per region from annotationmakeGenomicState
Build model matrices for differential expressionmakeModels
Merge results from different chromosomesmergeResults
Transform and split the datapreprocessCoverage
Identify regions data by a coverage filter and get a count matrix from BigWig filesrailMatrix
Construct full paths to a group of raw input filesrawFiles
Identify regions data by a coverage filter and get a count matrixregionMatrix
Calculate adjustments for library sizesampleDepth