dagLogo Vignette
Introduction | Step-by-step guide on using dagLogo | First load the library dagLogo | Step 1: Fetching peptide sequences from BioMart | Case 1: Fetch sequences using the fetchSequence function in biomaRt package given a list of gene identifiers and the corresponding positions of the anchoring AA. | Case 2: Fetch sequences using the fetchSequence function in biomaRt package given a list of gene identifiers and the corresponding peptide subsequences of interest with the anchoring AA marked such as asterisks or lower case of one or more AA letters. | Case 3: Prepare an object of dagPeptides using prepareProteome and formatSequence functions sequentially given a list of unaligned/aligned ungapped peptide sequences. | Step 2: Building background models | Step 3: Statistical significance test for differential usage of amino acids with or without grouping | Step 4: Visualize significance test results | Session Info