Package: NADfinder 1.37.0

Jianhong Ou

NADfinder: Call wide peaks for sequencing data

Nucleolus is an important structure inside the nucleus in eukaryotic cells. It is the site for transcribing rDNA into rRNA and for assembling ribosomes, aka ribosome biogenesis. In addition, nucleoli are dynamic hubs through which numerous proteins shuttle and contact specific non-rDNA genomic loci. Deep sequencing analyses of DNA associated with isolated nucleoli (NAD- seq) have shown that specific loci, termed nucleolus- associated domains (NADs) form frequent three- dimensional associations with nucleoli. NAD-seq has been used to study the biological functions of NAD and the dynamics of NAD distribution during embryonic stem cell (ESC) differentiation. Here, we developed a Bioconductor package NADfinder for bioinformatic analysis of the NAD-seq data, including baseline correction, smoothing, normalization, peak calling, and annotation.

Authors:Jianhong Ou, Haibo Liu, Jun Yu, Hervé Pagès, Paul Kaufman, Lihua Julie Zhu

NADfinder_1.37.0.tar.gz
NADfinder_1.37.0.zip(r-4.7-any)NADfinder_1.37.0.zip(r-4.6-any)NADfinder_1.37.0.zip(r-4.5-any)
NADfinder_1.37.0.tgz(r-4.6-any)NADfinder_1.37.0.tgz(r-4.5-any)
NADfinder_1.37.0.tar.gz(r-4.7-any)NADfinder_1.37.0.tar.gz(r-4.6-any)
NADfinder_1.37.0.tgz(r-4.6-emscripten)
manual.pdf |manual.html✨
DESCRIPTION |NEWS
card.svg |card.png
NADfinder/json (API)

# Install 'NADfinder' in R:

install.packages('NADfinder', repos = c('https://bioc.r-universe.dev', 'https://cloud.r-project.org'))

Datasets:

single.count - Counts data for chromosome 18 for an experiment of a single pair of samples
triplicate.count - Counts data for chromosome 18 for an expriment with triplicates

On BioConductor:NADfinder-1.37.0(bioc 3.24)NADfinder-1.36.0(bioc 3.23)

This package does not link to any Github/Gitlab/R-forge repository. No issue tracker or development information is available.

sequencing dnaseq generegulation peakdetection

4.00 score 1 scripts 645 downloads 17 exports 194 dependencies

Last updated from:b87312bce6. Checks:1 ERROR, 7 NOTE, 2 OK. Indexed: yes.

Target	Result	Time
bioc-checks	ERROR	271
linux-devel	NOTE	554
source / vignettes	OK	422
linux-release	NOTE	644
macos-release	NOTE	439
macos-oldrel	NOTE	331
windows-devel	NOTE	518
windows-release	NOTE	538
windows-oldrel	NOTE	491
wasm-release	OK	192

Exports:backgroundCorrection butterFilter callPeaks cumulativePercentage exportSignals getCorrelations groupZscores IntersectionNotStrict log2se peakdet plotSig smoothRatiosByChromosome tileCount tileCount2 transformData trimPeaks zscoreOverBck

Dependencies:abind ade4 AnnotationDbi AnnotationFilter AnnotationHub askpass ATACseqQC base64enc baseline BH Biobase BiocBaseUtils BiocFileCache BiocGenerics BiocIO biocmake BiocManager BiocParallel BiocVersion biomaRt Biostrings bit bit64 bitops blob BSgenome bslib cachem caTools ChIPpeakAnno cigarillo cli codetools corrplot cpp11 crayon csaw curl data.table DBI dbplyr DelayedArray DEoptimR digest dir.expiry DirichletMultinomial dplyr edgeR EmpiricalBrownsMethod ensembldb evaluate farver fastmap filelock fontawesome formatR fs futile.logger futile.options generics GenomeInfoDb GenomicAlignments GenomicFeatures GenomicRanges GenomicScores ggplot2 glue graph grImport gtable gtools h5mread HDF5Array highr hms htmltools htmlwidgets httr httr2 InteractionSet IRanges isoband jquerylib jsonlite KEGGREST KernSmooth knitr labeling lambda.r lattice lazyeval lifecycle limma locfit magrittr MASS mathjaxr Matrix MatrixGenerics MatrixModels matrixStats memoise metap metapod mime mnormt motifStack multcomp multtest mutoss mvtnorm numDeriv openssl pillar pixmap pkgconfig plotrix png polynom prettyunits progress ProtGenerics purrr pwalign qqconf quantreg R6 randomForest rappdirs RBGL rbibutils RColorBrewer Rcpp RcppArmadillo RcppThread RCurl Rdpack regioneR restfulr rhdf5 rhdf5filters Rhdf5lib Rhtslib rjson rlang rmarkdown robustbase Rsamtools RSQLite rtracklayer S4Arrays S4Vectors S7 sandwich sass scales Seqinfo seqLogo signal sn snow sp SparseArray SparseM statmod strawr stringi stringr SummarizedExperiment survival sys TFBSTools TFisher TFMPvalue TH.data tibble tidyr tidyselect tinytex trackViewer txdbmaker UCSC.utils universalmotif utf8 vctrs VennDiagram viridisLite withr xfun XML xml2 XVector yaml zoo

NADfinder Guide

Rendered fromNADfinder.Rmdusingknitr::rmarkdown

Last update: 2019-08-15
Started: 2017-03-31

Help page	Topics
Identify nucleolus-associated domains (NADs) from NAD-seq	NADfinder-package NADfinder
Correct ratios for background	backgroundCorrection
Low pass filter on ratios by butterworth filter	butterFilter
Call peaks using transformed, background corrected, and smoothed ratios with biological replicates	callPeaks
Perform overlap queries between reads and genome by sliding windows Count reads over sliding windows.	computeLibSizeChrom
Plot the cumulative percentage of tag allocation	cumulativePercentage
Output signals for visualization	exportSignals
Get correlation coefficinets and p-values between biological replicates	getCorrelations
Calculate z-scores for each peak	groupZscores
Count reads overlapping genomic ranges	IntersectionNotStrict
calculate the log2 transformed ratios for SummarizedExperiment class	log2se
Detect peak positions	peakdet
Plot signals with ideograms	plotSig
Counts data for chromosome 18 for an experiment of a single pair of samples	single.count
Backgound correction and signal smoothing per chromosome	smoothRatiosByChromosome
Perform overlap queries between reads and genome by windows	tileCount
Perform overlap queries between reads and genome by sliding windows Count reads over sliding windows.	tileCount2
transform counts to log2 cpm ratios, log2 ratios or log2 odds ratios	transformData
Trim peaks	trimPeaks
Counts data for chromosome 18 for an expriment with triplicates	triplicate.count
Z-scores over the background	zscoreOverBck

Package: NADfinder 1.37.0

NADfinder: Call wide peaks for sequencing data

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