Package: GSVA 2.7.7

Robert Castelo

GSVA: Gene Set Variation Analysis for Microarray and RNA-Seq Data

Gene Set Variation Analysis (GSVA) is a non-parametric, unsupervised method for estimating variation of gene set enrichment through the samples of a expression data set. GSVA performs a change in coordinate systems, transforming the data from a gene by sample matrix to a gene-set by sample matrix, thereby allowing the evaluation of pathway enrichment for each sample. This new matrix of GSVA enrichment scores facilitates applying standard analytical methods like functional enrichment, survival analysis, clustering, CNV-pathway analysis or cross-tissue pathway analysis, in a pathway-centric manner.

Authors:Robert Castelo [aut, cre], Justin Guinney [aut], Alexey Sergushichev [ctb], Pablo Sebastian Rodriguez [ctb], Axel Klenk [ctb], Chan Zuckerberg Initiative [fnd], Spanish Ministry of Science, Innovation and Universities [fnd]

GSVA_2.7.7.tar.gz
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GSVA_2.7.7.tgz(r-4.6-x86_64)GSVA_2.7.7.tgz(r-4.6-arm64)GSVA_2.7.7.tgz(r-4.5-x86_64)GSVA_2.7.7.tgz(r-4.5-arm64)
GSVA_2.7.7.tar.gz(r-4.7-arm64)GSVA_2.7.7.tar.gz(r-4.7-x86_64)GSVA_2.7.7.tar.gz(r-4.6-arm64)GSVA_2.7.7.tar.gz(r-4.6-x86_64)
GSVA_2.7.7.tgz(r-4.6-emscripten)
manual.pdf |manual.html
DESCRIPTION |NEWS
card.svg |card.png
GSVA/json (API)

# Install 'GSVA' in R:
install.packages('GSVA', repos = c('https://bioc.r-universe.dev', 'https://cloud.r-project.org'))

Bug tracker:https://github.com/rcastelo/gsva/issues

On BioConductor:GSVA-2.7.6(bioc 3.24)GSVA-2.6.2(bioc 3.23)

functionalgenomicsmicroarrayrnaseqpathwaysgenesetenrichmentgene-set-enrichmentgenomicspathway-enrichment-analysis

14.46 score 245 stars 21 packages 2.8k scripts 893 mentions 32 exports 95 dependencies

Last updated from:f78c675e7d. Checks:1 ERROR, 13 OK. Indexed: yes.

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linux-release-arm64OK558
linux-release-x86_64OK661
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macos-oldrel-arm64OK559
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Exports:computeGeneSetsOverlapdeduplicateGeneSetsdetailsfilterGeneSetsgeneIdsToGeneSetCollectiongeneSetsgeneSets<-geneSetSizesgsvagsvaAnnotationgsvaAnnotation<-gsvaBatchtoolsSlurmParamgsvaColRanksgsvaColScoresgsvaEnrichmentgsvaMapgsvaParamgsvaRanksgsvaReducegsvaRowNormgsvaScoresguessGeneIdTypeigsvaloadHDF5GSVAloadHDF5GSVAranksplageParamreadGMTsaveHDF5GSVAsaveHDF5GSVAranksspatCorssgseaParamzscoreParam

Dependencies:abindannotateAnnotationDbiaskpassassortheadbeachmatBHBiobaseBiocFileCacheBiocGenericsbiocmakeBiocParallelBiocSingularBiostringsbitbit64blobcachemclicodetoolscpp11crayoncurlDBIdbplyrDelayedArrayDelayedMatrixStatsdir.expirydplyrfastmapfilelockformatRfutile.loggerfutile.optionsgenericsGenomicRangesgluegraphGSEABaseh5mreadHDF5Arrayhttrhttr2IRangesirlbajsonliteKEGGRESTlambda.rlatticelifecyclemagickmagrittrMatrixMatrixGenericsmatrixStatsmemoisememusemimeopensslpillarpkgconfigpngpurrrR6rappdirsRcpprhdf5rhdf5filtersRhdf5librjsonrlangRSQLitersvdS4ArraysS4VectorsScaledMatrixSeqinfoSingleCellExperimentsnowSparseArraysparseMatrixStatsSpatialExperimentstringistringrSummarizedExperimentsystibbletidyrtidyselectutf8vctrswithrXMLxtableXVector

Running GSVA in an HPC environment
Introduction | Using a SLURM workload manager | Session information | References

Last update: 2026-06-30
Started: 2026-06-30

GSVA on proteomics data
Introduction | Load gene sets | Usage and benchmark with RNA-seq data | Usage and benchmark with proteomics data | Session information | References

Last update: 2026-05-25
Started: 2026-04-27

GSVA on single-cell RNA-seq data
Introduction | Import data | Quality control and pre-processing | Annotate cell types using GSVA | Read gene sets in GMT format | Add gene identifier type metadata | Build parameter object | Calculate GSVA scores | Using GSVA scores to assign cell types | Benchmarking | Session information | References

Last update: 2026-05-25
Started: 2025-10-27

GSVA: gene set variation analysis
Quick start | Introduction | Overview of the GSVA functionality | Gene set definitions and containers | Importing gene sets from GMT files | Quantification of pathway activity in bulk microarray and RNA-seq data | Example applications | Molecular signature identification | Differential expression at pathway level | Data exploration at gene level | Running GSVA | Data exploration at pathway level | Interactive web app | Contributing | Session information | References

Last update: 2026-02-19
Started: 2021-02-05

Readme and manuals

Help Manual

Help pageTopics
Compute gene-sets overlapcomputeGeneSetsOverlap computeGeneSetsOverlap,GeneSetCollection,character-method computeGeneSetsOverlap,list,character-method
Handling of Duplicated Gene Set NamesdeduplicateGeneSets
Filter gene setsfilterGeneSets filterGeneSets,GeneSetCollection-method filterGeneSets,list-method
Construct a GeneSetCollection object from a list of character vectorsgeneIdsToGeneSetCollection
Retrieve or Determine Gene SetsgeneSets geneSets,GsvaExprData-method geneSets,GsvaMethodParam-method geneSets,SingleCellExperiment-method geneSets,SpatialExperiment-method geneSets,SummarizedExperiment-method geneSetSizes geneSetSizes,GsvaExprData-method geneSetSizes,GsvaMethodParam-method
Gene Set Variation Analysisgsva gsva,gsvaParam-method gsva,plageParam-method gsva,ssgseaParam-method gsva,zscoreParam-method
Store and Retrieve Annotation MetadatagsvaAnnotation gsvaAnnotation,ExpressionSet-method gsvaAnnotation,GeneSetCollection-method gsvaAnnotation,GsvaExprData-method gsvaAnnotation,list-method gsvaAnnotation,SingleCellExperiment-method gsvaAnnotation,SpatialExperiment-method gsvaAnnotation,SummarizedExperiment-method gsvaAnnotation<- gsvaAnnotation<-,ExpressionSet,character-method gsvaAnnotation<-,ExpressionSet,GeneIdentifierType-method gsvaAnnotation<-,GsvaExprData,GeneIdentifierType-method gsvaAnnotation<-,list,GeneIdentifierType-method gsvaAnnotation<-,SingleCellExperiment,GeneIdentifierType-method gsvaAnnotation<-,SpatialExperiment,GeneIdentifierType-method gsvaAnnotation<-,SummarizedExperiment,GeneIdentifierType-method
GSVA enrichment data and visualizationgsvaEnrichment
'GsvaExprData' classGsvaExprData GsvaExprData-class
'GsvaGeneSets' classGsvaGeneSets-class
MapReduce parallelization for HPC environmentsgsvaBatchtoolsSlurmParam gsvaMap gsvaReduce
'GsvaMethodParam' classdetails,GsvaMethodParam-method details,gsvaParam-method details,ssgseaParam-method GsvaMethodParam-class
'gsvaParam' classanyNA,gsvaParam-method geneSets<- geneSets<-,gsvaParam,GsvaGeneSets-method gsvaParam gsvaParam-class gsvaRanksParam-class
GSVA ranks and scoresgsvaColRanks gsvaColScores gsvaRowNorm
Guess the gene identifier type from a list of character vectorsguessGeneIdType
Gene Set Variation Analysisigsva
'plageParam' classplageParam plageParam-class
Import Gene Sets from a GMT FilereadGMT
Save/load GSVA output to disk using HDF5 formatloadHDF5GSVA saveHDF5GSVA
Compute Spatial Autocorrelation for SpatialExperiment objectsspatCor spatCor,SpatialExperiment-method
'ssgseaParam' classanyNA,ssgseaParam-method ssgseaParam ssgseaParam-class
'zscoreParam' classzscoreParam zscoreParam-class