twoddpcr: A package for Droplet Digital PCR analysis
Introduction | Installing the twoddpcr package | Loading the twoddpcr package | Using the in-built dataset | Basic plots | Plotting the droplets and existing classifications | Independent linear gating on the channels (thresholdClassify) | Classifying using the k-means algorithm (kmeansClassify) | Adding "rain" | Creating a summary | Analysis of the data | Comparison of classification methods | Discussion | Other classification tools | Classifying using the k-NN algorithm (knnClassify) | Classifying the four 'corners' of a plot (gridClassify) | Adding rain with sdRain | Custom classifications | Appendix | Shiny-based GUI for non-R users | Exporting droplet amplitudes from QuantaSoft to CSV files | Using other datasets | Problems reading files | Citing twoddpcr | Further reading | Session information | References