Package: systemPipeR 2.13.0

Thomas Girke

systemPipeR: systemPipeR: Workflow Environment for Data Analysis and Report Generation

systemPipeR is a multipurpose data analysis workflow environment that unifies R with command-line tools. It enables scientists to analyze many types of large- or small-scale data on local or distributed computer systems with a high level of reproducibility, scalability and portability. At its core is a command-line interface (CLI) that adopts the Common Workflow Language (CWL). This design allows users to choose for each analysis step the optimal R or command-line software. It supports both end-to-end and partial execution of workflows with built-in restart functionalities. Efficient management of complex analysis tasks is accomplished by a flexible workflow control container class. Handling of large numbers of input samples and experimental designs is facilitated by consistent sample annotation mechanisms. As a multi-purpose workflow toolkit, systemPipeR enables users to run existing workflows, customize them or design entirely new ones while taking advantage of widely adopted data structures within the Bioconductor ecosystem. Another important core functionality is the generation of reproducible scientific analysis and technical reports. For result interpretation, systemPipeR offers a wide range of plotting functionality, while an associated Shiny App offers many useful functionalities for interactive result exploration. The vignettes linked from this page include (1) a general introduction, (2) a description of technical details, and (3) a collection of workflow templates.

Authors:Thomas Girke

systemPipeR_2.13.0.tar.gz
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systemPipeR.pdf |systemPipeR.html
systemPipeR/json (API)
NEWS

# Install 'systemPipeR' in R:
install.packages('systemPipeR', repos = c('https://bioc.r-universe.dev', 'https://cloud.r-project.org'))

Peer review:

Bug tracker:https://github.com/tgirke/systempiper/issues

On BioConductor:systemPipeR-2.13.0(bioc 3.21)systemPipeR-2.12.0(bioc 3.20)

geneticsinfrastructuredataimportsequencingrnaseqriboseqchipseqmethylseqsnpgeneexpressioncoveragegenesetenrichmentalignmentqualitycontrolimmunooncologyreportwritingworkflowstepworkflowmanagement

11.55 score 52 stars 3 packages 336 scripts 2.0k downloads 27 mentions 161 exports 100 dependencies

Last updated 2 months agofrom:94357f24a2. Checks:OK: 1 ERROR: 6. Indexed: yes.

TargetResultDate
Doc / VignettesOKNov 27 2024
R-4.5-winERRORNov 27 2024
R-4.5-linuxERRORNov 27 2024
R-4.4-winERRORNov 27 2024
R-4.4-macERRORNov 27 2024
R-4.3-winERRORNov 27 2024
R-4.3-macERRORNov 27 2024

Exports:addResourcesalignStatsappendCodeLine<-appendParamappendParam2appendStep<-as.listbaseCommandcatlistcatmapcheck.outfilescheck.outputcltclusterRuncmdlistcmdToCwlcmdToCwl<-codeChunkStartcodeLinecombineVarReportscomplexitylevelsconfig.paramconfigWFcopyEnvircorescountRangesetcreateParamcreateParamFilescwlFilesUpdatedependencydependency<-evalCodefeatureCoveragefeaturetypeCountsfilesfilterDEGsfilterVarsgenFeaturesgetColumngoBarplotGOCluster_ReportGOHyperGAllGOHyperGAll_SimplifyGOHyperGAll_SubsetidconvimportWFinfile1infile2inputinputvarsintersectlistintersectmatrixlinewiseLineWiselistCmdModuleslistCmdToolsloadWFloadWorkflowmakeCATdbmergeBamByFactormodulemoduleAvailmoduleClearmoduleInitmodulelistmoduleloadmodulesmoduleUnloadolBarplotolRangesotheroutfile1outfilesoutpathsoutputoutput_updateoverLapperplotfeatureCoverageplotfeaturetypeCountsplotWFplotwfOutputpredORFpreprocessReadsprintParamprintParam2projectInforeadCompreferenceremoveParam2renameParamrenameParam2renameStep<-renderLogsrenderPlotwfrenderReportrenderWFreplaceCodeLine<-replaceParamreplaceParam2replaceStep<-resultsreturnRPKMrmdPathrun_DESeq2run_edgeRrunCommandlinerunDiffrunInforunWFsal2bashsal2rmdSampleNamescaleRangesSESE<-seeFastqseeFastqPlotsetlistshowshowDFsoftwareSPRprojectstatusstatusWFstepNamestepsWFsubsetsubsetParamsubsetWFsymLink2bamsysargssysargs2sysargslistSYSargsListsystemArgstargetstargets.as.dftargets.as.listtargetsheadertargetsintargetsouttargetsWFtrimbatchtryCLtryCMDtryPathupdateColumn<-updateWFvariantReportvarSummaryvennlistvennPlotviewEnvirwfwrite_SYSargsListwriteParamFileswriteTargetswriteTargetsoutwriteTargetsRefyamlinputyamlinput<-

Dependencies:abindaskpassbase64encBHBiobaseBiocGenericsBiocParallelBiostringsbitopsbslibcachemclicodetoolscolorspacecpp11crayoncurlDelayedArraydeldirdigestevaluatefansifarverfastmapfontawesomeformatRfsfutile.loggerfutile.optionsgenericsGenomeInfoDbGenomeInfoDbDataGenomicAlignmentsGenomicRangesggplot2gluegtablehighrhtmltoolshtmlwidgetshttrhwriterinterpIRangesisobandjpegjquerylibjsonliteknitrlabelinglambda.rlatticelatticeExtralifecyclemagrittrMASSMatrixMatrixGenericsmatrixStatsmemoisemgcvmimemunsellnlmeopensslpillarpkgconfigpngpwalignR6rappdirsRColorBrewerRcppRcppEigenRhtslibrlangrmarkdownRsamtoolsS4ArraysS4VectorssassscalesShortReadsnowSparseArraystringistringrSummarizedExperimentsystibbletinytexUCSC.utilsutf8vctrsviridisLitewithrxfunXVectoryamlzlibbioc

systemPipeR: Workflow Environment for Data Analysis and Report Generation

Rendered fromsystemPipeR.Rmdusingknitr::rmarkdownon Nov 27 2024.

Last update: 2024-09-01
Started: 2015-07-27

systemPipeR: Workflow Templates

Rendered fromsystemPipeR_workflows.Rmdusingknitr::rmarkdownon Nov 27 2024.

Last update: 2024-07-28
Started: 2019-10-15

Readme and manuals

Help Manual

Help pageTopics
systemPipeR package for Workflow EnvironmentsystemPipeR-package
Extension accessor methods for SummarizedExperiment objectaddAssay addAssay,SummarizedExperiment-method addAssay-methods addMetadata addMetadata,SummarizedExperiment-method addMetadata-methods
Alignment statisticsalignStats
Class '"catDB"'catDB-class catlist,catDB-method catmap,catDB-method coerce,list,catDB-method idconv,catDB-method names,catDB-method show,catDB-method
catDB accessor methodscatlist catlist-methods catmap catmap-methods idconv idconv-methods
Checking if the outfiles files existcheck.outfiles check.output
Submit command-line tools to clusterclusterRun
Adding param fileconfig.param
Workflow Steps SelectionconfigWF
Read counting for several range setscountRangeset
createParamcreateParam createParamFiles writeParamFiles
Update CWL description filescwlFilesUpdate
Class '"EnvModules"'$,EnvModules-method available_modules,EnvModules-method coerce,EnvModules,list-method coerce,list,EnvModules-method default_modules,EnvModules-method EnvModules,EnvModules-method EnvModules-class loaded_modules,EnvModules-method modulecmd,EnvModules-method names,EnvModules-method show,EnvModules-method [,EnvModules,ANY,ANY,ANY-method [[,EnvModules,ANY,ANY-method [[,EnvModules,ANY,missing-method [[<-,EnvModules,ANY,ANY,ANY-method [[<-,EnvModules,ANY,ANY-method [[<-,EnvModules-method
Toggles option 'eval' on the RMarkdown filesevalCode
Genome read coverage by transcript modelsfeatureCoverage
Plot read distribution across genomic featuresfeaturetypeCounts
Filter and plot DEG resultsfilterDEGs
Filter VCF filesfilterVars
Generate feature ranges from TxDbgenFeatures
GO term enrichment analysis for large numbers of gene setsgoBarplot GOCluster_Report GOHyperGAll GOHyperGAll_Simplify GOHyperGAll_Subset makeCATdb
Import R Markdown file as workflowimportWF
Class '"INTERSECTset"'as.list,INTERSECTset-method coerce,list,INTERSECTset-method complexitylevels,INTERSECTset-method intersectlist,INTERSECTset-method intersectmatrix,INTERSECTset-method INTERSECTset-class length,INTERSECTset-method names,INTERSECTset-method setlist,INTERSECTset-method show,INTERSECTset-method
Class '"LineWise"'$,LineWise-method appendCodeLine,LineWise-method appendCodeLine<-,LineWise-method codeChunkStart codeChunkStart,LineWise-method codeLine,LineWise-method coerce,LineWise,list-method coerce,list,LineWise-method dependency,LineWise-method files,LineWise-method length,LineWise-method LineWise linewise linewise,LineWise-method LineWise-class names,LineWise-method replaceCodeLine,LineWise-method replaceCodeLine<-,LineWise-method rmdPath rmdPath,LineWise-method runInfo,LineWise-method show,LineWise-method status,LineWise-method stepName,LineWise-method [,LineWise,ANY,ANY,ANY-method [[,LineWise,ANY,missing-method [[<-,LineWise,ANY,ANY,ANY-method [[<-,LineWise,ANY,ANY-method
List/check the existence of command-line tools of a workflowlistCmdModules listCmdTools
Constructs SYSargs2 object from CWL param and targets filesloadWF loadWorkflow renderWF updateWF
Merge BAM files based on factormergeBamByFactor
Interface to allow full use of the Environment Modules system for Unixmodule moduleAvail moduleClear moduleInit modulelist moduleload moduleUnload
Bar plot for intersect setsolBarplot
Identify Range Overlaps for IRanges and GRanges ObjectolRanges
Updates the output files paths in the 'SYSargs2' objectoutput_update
Set Intersect and Venn Diagram Functionscomplexitylevels complexitylevels-methods intersectlist intersectlist-methods intersectmatrix intersectmatrix-methods overLapper setlist setlist-methods vennlist vennlist-methods
Plot feature coverage resultsplotfeatureCoverage
Plot read distribution across genomic featuresplotfeaturetypeCounts
Visualize SPR workflow and statusplotWF plotwfOutput renderPlotwf
Predict ORFspredORF
Run custom read preprocessing functionspreprocessReads
Accessories function to modify the Command-line Version 1appendParam printParam renameParam replaceParam subsetParam
Accessories function to modify the Command-line Version 2appendParam2 printParam2 removeParam2 renameParam2 replaceParam2
Import sample comparisons from targets filereadComp
Render RMarkdown Logs ReportrenderLogs
Render RMarkdown ReportrenderReport
RPKM NormalizationreturnRPKM
Runs DESeq2run_DESeq2
Runs edgeRrun_edgeR
Execute SYSargs and SYSargs2runCommandline
Differential abundance analysis for many range setsrunDiff
Execute SYSargsListrunWF
Translate SYSargsList back to a bash workflowsal2bash
Translate SYSargsList back to a workflow template Rmarkdown filesal2rmd
Scale spliced ranges to genome coordinatesscaleRanges
Quality reports for FASTQ filesseeFastq seeFastqPlot
Create an HTML table using DT package with fixed columnsshowDF
Workflow Project InitiationSPRproject
Subsetting SYSargs2 class slotssubsetWF
Symbolic links for IGVsymLink2bam
SYSargs accessor methodscores cores-methods infile1 infile1-methods infile2 infile2-methods modules-methods other other-methods outfile1 outfile1-methods outpaths outpaths-methods reference reference-methods results results-methods SampleName-methods software software-methods sysargs sysargs-methods targetsheader-methods targetsin targetsin-methods targetsout targetsout-methods
Class '"SYSargs"'coerce,list,SYSargs-method cores,SYSargs-method infile1,SYSargs-method infile2,SYSargs-method length,SYSargs-method modules,SYSargs-method names,SYSargs-method other,SYSargs-method outfile1,SYSargs-method outpaths,SYSargs-method reference,SYSargs-method results,SYSargs-method SampleName,SYSargs-method show,SYSargs-method software,SYSargs-method sysargs,SYSargs-method SYSargs-class targetsheader,SYSargs-method targetsin,SYSargs-method targetsout,SYSargs-method [,SYSargs,ANY,ANY,ANY-method
Class '"SYSargs2"'$,SYSargs2-method baseCommand,SYSargs2-method clt clt,SYSargs2-method cmdlist,SYSargs2-method cmdToCwl cmdToCwl,SYSargs2-method cmdToCwl<- cmdToCwl<-,SYSargs2-method coerce,list,SYSargs2-method coerce,SYSargs2,list-method files files,SYSargs2-method infile1,SYSargs2-method infile2,SYSargs2-method input input,SYSargs2-method inputvars inputvars,SYSargs2-method internal_outfiles internal_outfiles,SYSargs2-method length,SYSargs2-method modules modules,SYSargs2-method names,SYSargs2-method output output,SYSargs2-method SampleName,SYSargs2-method show,SYSargs2-method status status,SYSargs2-method sysargs2 sysargs2,SYSargs2-method SYSargs2-class targets targets,SYSargs2-method targetsheader,SYSargs2-method wf wf,SYSargs2-method yamlinput,SYSargs2-method yamlinput<-,SYSargs2,ANY,ANY-method yamlinput<-,SYSargs2-method [,SYSargs2,ANY,ANY,ANY-method [[,SYSargs2,ANY,missing-method [[<-,SYSargs2,ANY,ANY,ANY-method [[<-,SYSargs2,ANY,ANY-method
SYSargsList ConstructorSYSargsList
Class '"SYSargsList"'$,SYSargsList-method addResources addResources,SYSargsList-method appendCodeLine<- appendCodeLine<-,SYSargsList-method appendStep<- appendStep<-,SYSargsList-method baseCommand baseCommand,SYSargsList-method cmdlist cmdlist,SYSargsList-method codeLine codeLine,SYSargsList-method coerce,list,SYSargsList-method coerce,SYSargsList,list-method copyEnvir copyEnvir,SYSargsList-method dependency dependency,SYSargsList-method dependency<- dependency<-,SYSargsList-method getColumn getColumn,SYSargsList-method length,SYSargsList-method names,SYSargsList-method outfiles outfiles,SYSargsList-method projectInfo projectInfo,SYSargsList-method renameStep<- renameStep<-,SYSargsList-method replaceCodeLine<- replaceCodeLine<-,SYSargsList-method replaceStep<- replaceStep<-,SYSargsList-method runInfo runInfo,SYSargsList-method SampleName SampleName,SYSargsList-method SE SE,SYSargsList-method SE<- SE<-,SYSargsList-method show,SYSargsList-method statusWF statusWF,SYSargsList-method stepName stepName,SYSargsList-method stepsWF stepsWF,SYSargsList-method subset subset,SYSargsList-method sysargslist sysargslist,SYSargsList-method SYSargsList-class targetsheader targetsheader,SYSargsList-method targetsWF targetsWF,SYSargsList-method targets_connection targets_connection,SYSargsList-method updateColumn<- updateColumn<-,SYSargsList-method viewEnvir viewEnvir,SYSargsList-method yamlinput yamlinput,SYSargsList-method yamlinput<- yamlinput<-,SYSargsList-method [,SYSargsList,ANY,ANY,ANY-method [[,SYSargsList,ANY,missing-method [[<-,SYSargsList,ANY,ANY,ANY-method [[<-,SYSargsList,ANY,ANY-method
Constructs SYSargs object from param and targets filessystemArgs
Convert targets to list or data.frametargets.as.df targets.as.list
Genome read coverage by transcript modelstrimbatch
Collect information about the third-party softwaretryCL tryCMD
Validation of the files or directoriestryPath
Generate Variant ReportcombineVarReports variantReport varSummary
Plot 2-5 way Venn diagramsvennPlot
Class '"VENNset"'as.list,VENNset-method coerce,list,VENNset-method complexitylevels,VENNset-method intersectmatrix,VENNset-method length,VENNset-method names,VENNset-method setlist,VENNset-method show,VENNset-method vennlist,VENNset-method VENNset-class
Writeout SYSargsList objectwrite_SYSargsList
Write updated targets out to file from 'SYSargsList'writeTargets
Write updated targets out to filewriteTargetsout
Generate targets file with referencewriteTargetsRef