Package: easyRNASeq 2.43.0

Nicolas Delhomme

easyRNASeq: Count summarization and normalization for RNA-Seq data

Calculates the coverage of high-throughput short-reads against a genome of reference and summarizes it per feature of interest (e.g. exon, gene, transcript). The data can be normalized as 'RPKM' or by the 'DESeq' or 'edgeR' package.

Authors:Nicolas Delhomme, Ismael Padioleau, Bastian Schiffthaler, Niklas Maehler

easyRNASeq_2.43.0.tar.gz
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easyRNASeq.pdf |easyRNASeq.html
easyRNASeq/json (API)
NEWS

# Install 'easyRNASeq' in R:
install.packages('easyRNASeq', repos = c('https://bioc.r-universe.dev', 'https://cloud.r-project.org'))

Peer review:

Datasets:

On BioConductor:easyRNASeq-2.43.0(bioc 3.21)easyRNASeq-2.42.0(bioc 3.20)

This package does not link to any Github/Gitlab/R-forge repository. No issue tracker or development information is available.

geneexpressionrnaseqgeneticspreprocessingimmunooncology

5.43 score 1 packages 15 scripts 384 downloads 17 mentions 87 exports 102 dependencies

Last updated 23 days agofrom:d32c68e31e. Checks:OK: 1 WARNING: 2 NOTE: 4. Indexed: yes.

TargetResultDate
Doc / VignettesOKNov 19 2024
R-4.5-winWARNINGNov 19 2024
R-4.5-linuxWARNINGNov 19 2024
R-4.4-winNOTENov 19 2024
R-4.4-macNOTENov 19 2024
R-4.3-winNOTENov 19 2024
R-4.3-macNOTENov 19 2024

Exports:alignDataannotParamAnnotParamassayassaysBamFileListbamParamBamParambarcodePlotbasenamechastityFilterchromosomeFilterchrSizechrSize<-colDatacolnamescomposecountBycreateSyntheticTranscriptsdatasourcedemultiplexeasyRNASeqestimateDispersionsestimateSizeFactorsexonCountsfeatureCountsfetchCoveragefetchDatafileNamefileName<-geneCountsgeneModelgeneModel<-genomicAnnotationgenomicAnnotation<-getAnnotationgetBamFileListGRangesIRangeslibrarySizelibrarySize<-metadatanaPositionFilternFilterorganismNameorganismName<-pairedparallelizeplotNormalizationFactorsprecisionrangesreadAlignedreadCountsreadCounts<-readCoveragereadCoverage<-readLengthreadLength<-reduceRnaSeqParamrowRangesRPKMseqinfoseqlengthsseqlevelsseqlevels<-seqnamesseqnames<-simpleRNASeqsplitsrFilterSRFilterResultstrandstrand<-strandedstrandProtocolSummarizedExperimenttranscriptCountstutorialDatatypeunsafeAppendvalidatevignetteDatawidthwriteFastqwriteGff3yieldSize

Dependencies:abindAnnotationDbiaskpassBHBiobaseBiocFileCacheBiocGenericsBiocParallelbiomaRtBiostringsbitbit64bitopsblobcachemclicodetoolscpp11crayoncurlDBIdbplyrDelayedArraydeldirdigestdplyredgeRfansifastmapfilelockformatRfutile.loggerfutile.optionsgenericsGenomeInfoDbGenomeInfoDbDatagenomeIntervalsGenomicAlignmentsGenomicRangesgluehmshttrhttr2hwriterinterpintervalsIRangesjpegjsonliteKEGGRESTlambda.rlatticelatticeExtralifecyclelimmalocfitLSDmagrittrMASSMatrixMatrixGenericsmatrixStatsmemoisemimeopensslpillarpkgconfigplogrpngprettyunitsprogresspurrrpwalignR6rappdirsRColorBrewerRcppRcppEigenRhtslibrlangRsamtoolsRSQLiteS4ArraysS4VectorsShortReadsnowSparseArraystatmodstringistringrSummarizedExperimentsystibbletidyrtidyselectUCSC.utilsutf8vctrswithrxml2XVectorzlibbioc

A walkthrough the easyRNASeq package functionalities

Rendered fromsimpleRNASeq.Rmdusingknitr::rmarkdownon Nov 19 2024.

Last update: 2019-11-19
Started: 2015-12-23

R / Bioconductor for High Throughput Sequence Analysis

Rendered fromeasyRNASeq.Rnwusingutils::Sweaveon Nov 19 2024.

Last update: 2019-11-19
Started: 2012-02-14

Readme and manuals

Help Manual

Help pageTopics
Class "AnnotParam"AnnotParam class AnnotParam-class AnnotParamCharacter-class AnnotParamObject-class
Class "BamParam"BamParam class BamParam-class
Extend the basename function to display Rsamtools BamFile class basenamebasename basename methods basename,BamFile-method basename,BamFileList-method
Manages the data necessary for the examples using BiocFileCache.get_cache .get_cache,ANY-method BiocFileCache methods fetchData fetchData,character-method tutorialData tutorialData,ANY-method vignetteData vignetteData,ANY-method
Methods to create synthetic transcriptscreateSyntheticTranscripts createSyntheticTranscripts,AnnotParamCharacter-method createSyntheticTranscripts,character-method
The following function are defunct: • 'easyRNASeq' • 'fetchCoverage' • 'fetchAnnotation' • 'knownOrganisms'Defunct functions easyRNASeq easyRNASeq,RNAseq-method fetchAnnotation fetchCoverage fetchCoverage,RNAseq-method knownOrganisms organismName organismName,RNAseq-method organismName<- organismName<-,RNAseq-method
Accessors for RNAseq classaccessors chrSize chrSize,RNAseq-method chrSize<- chrSize<-,RNAseq,integer-method chrSize<-,RNAseq,list-method easyRNASeq accessors fileName fileName,RNAseq-method fileName<- fileName<-,RNAseq-method geneModel geneModel,RNAseq-method geneModel<- geneModel<-,RNAseq-method genomicAnnotation genomicAnnotation,RNAseq-method genomicAnnotation<- genomicAnnotation<-,RNAseq-method librarySize librarySize,RNAseq-method librarySize<- librarySize<-,RNAseq-method readCounts readCounts,RNAseq-method readCounts<- readCounts<-,RNAseq-method readCoverage readCoverage,RNAseq-method readCoverage<- readCoverage<-,RNAseq-method readIslands readIslands,RNAseq-method readIslands<- readIslands<-,RNAseq-method readLength readLength,RNAseq-method readLength<- readLength<-,RNAseq-method seqnames,RNAseq-method
Get genic annotation from a gff3/gtf file or using biomaRteasyRNASeq annotation methods getAnnotation getAnnotation,AnnotParam-method
Accessors for AnnotParam classAnnotParam-accessors datasource datasource,AnnotParam-method easyRNASeq AnnotParam accessors type,AnnotParam-method
AnnotParam constructorAnnotParam AnnotParam,character-method AnnotParam,GRanges-method AnnotParam,missing-method easyRNASeq AnnotParam constructor
Accessors for BamParam classBamParam-accessors easyRNASeq BamParam accessors paired paired,BamParam-method stranded stranded,BamParam-method strandProtocol strandProtocol,BamParam-method yieldSize yieldSize,BamParam-method
BamParam constructorBamParam BamParam,ANY-method easyRNASeq BamParam constructor
easyRNASeq count table correction to RPKMeasyRNASeq correction methods RPKM RPKM,matrix,ANY,vector,vector-method RPKM,RNAseq,ANY,ANY,ANY-method RPKM,RNAseq-method
Compute the coverage from a Short Read Alignment fileeasyRNASeq coverage methods fetchCoverage-deprecated
Defunct annotation functioneasyRNASeq defunct annotation methods fetchAnnotation-defunct knownOrganisms-defunct
Extension of the GenomicRanges packagecolnames colnames,GRanges-method colnames,GRangesList-method easyRNASeq GenomicRanges package extension unsafeAppend unsafeAppend,GAlignments,GAlignments-method
Identify expressed regions de-novoeasyRNASeq island methods findIslands findIslands,RNAseq-method
Accessors for RnaSeqParam classannotParam annotParam,RnaSeqParam-method bamParam bamParam,RnaSeqParam-method countBy countBy,RnaSeqParam-method datasource,RnaSeqParam-method easyRNASeq RnaSeqParam accessors paired,RnaSeqParam-method precision precision,RnaSeqParam-method RnaSeqParam-accessors stranded,RnaSeqParam-method strandProtocol,RnaSeqParam-method yieldSize,RnaSeqParam-method
RnaSeqParam constructoreasyRNASeq RnaSeqParam constructor RnaSeqParam RnaSeqParam,ANY-method
Count methods for RNAseq objecteasyRNASeq summarization methods exonCounts exonCounts,RNAseq-method featureCounts featureCounts,RNAseq-method geneCounts geneCounts,RNAseq-method islandCounts islandCounts,RNAseq-method transcriptCounts transcriptCounts,RNAseq-method
Dataset included in the packageeasyRNASeq-datasets RobinsonDelhomme2014
easyRNASeq methodeasyRNASeq,character-method easyRNASeq-defunct
Extension for the edgeR packageedgeR additional methods plotNormalizationFactors plotNormalizationFactors,DGEList,character,character-method
Extend the file.exists function to check the path slot of a Rsamtools BamFile class for existencefile.exists file.exists methods file.exists,BamFile-method
Extension for the genomeIntervals packagegenomeIntervals additional methods type,Genome_intervals-method
Get a BamFileList from a list of filenamesgetBamFileList getBamFileList,character,character-method getBamFileList,character,missing-method
Extension of the IRanges packageIRanges additional methods ranges ranges,RNAseq-method
parallel additional methodsparallel additional methods parallelize parallelize,BamFileList,function-method parallelize,GRangesList,function-method parallelize,list,function-method parallelize,vector,function-method
Pretty print the content of classes from the easyRNASeq package.print print methods print,AnnotParam-method print,BamParam-method print,RNAseq-method print,RnaSeqParam-method
Class "RNAseq"RNAseq RNAseq class RNAseq-class
Class "RnaSeqParam"RnaSeqParam class RnaSeqParam-class
Methods extending the ShortRead package functionalitiesalignData barcodePlot barcodePlot,AlignedRead-method barcodePlot,DNAStringSet-method barcodePlot,ShortReadQ-method chastityFilter chastityFilter,SRFilter-method demultiplex demultiplex,AlignedRead-method demultiplex,DNAStringSet-method demultiplex,ShortReadQ-method naPositionFilter naPositionFilter,SRFilter-method ShortRead additional methods
Display the content of classes from the easyRNASeq package.show methods show,AnnotParam-method show,BamParam-method show,RNAseq-method show,RnaSeqParam-method
simpleRNASeq methodsimpleRNASeq simpleRNASeq,BamFileList,RnaSeqParam-method
Extension of the Rsamtools packagevalidate validate,BamFile-method validate,BamFileList-method