Package: bsseq 1.43.1

Kasper Daniel Hansen

bsseq: Analyze, manage and store whole-genome methylation data

A collection of tools for analyzing and visualizing whole-genome methylation data from sequencing. This includes whole-genome bisulfite sequencing and Oxford nanopore data.

Authors:Kasper Daniel Hansen [aut, cre], Peter Hickey [aut], Hervé Pagès [ctb], Aaron Lun [ctb]

bsseq_1.43.1.tar.gz
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bsseq.pdf |bsseq.html
bsseq/json (API)
NEWS

# Install 'bsseq' in R:
install.packages('bsseq', repos = c('https://bioc.r-universe.dev', 'https://cloud.r-project.org'))

Peer review:

Bug tracker:https://github.com/kasperdanielhansen/bsseq/issues

Uses libs:
  • c++– GNU Standard C++ Library v3
Datasets:
  • BS.chr22 - Whole-genome bisulfite sequencing for chromosome 22 from Lister et al.

On BioConductor:bsseq-1.43.0(bioc 3.21)bsseq-1.42.0(bioc 3.20)

dnamethylationcpp

12.28 score 36 stars 16 packages 664 scripts 2.5k downloads 38 mentions 55 exports 80 dependencies

Last updated 6 days agofrom:1cce9a0102. Checks:OK: 1 NOTE: 8. Indexed: yes.

TargetResultDate
Doc / VignettesOKDec 17 2024
R-4.5-win-x86_64NOTEDec 17 2024
R-4.5-linux-x86_64NOTEDec 17 2024
R-4.4-win-x86_64NOTEDec 17 2024
R-4.4-mac-aarch64NOTEDec 17 2024
R-4.4-mac-x86_64NOTENov 29 2024
R-4.3-win-x86_64NOTEDec 17 2024
R-4.3-mac-aarch64NOTEDec 17 2024
R-4.3-mac-x86_64NOTENov 29 2024

Exports:assayNamesassaysbinomialGoodnessOfFitBSmoothBSmooth.tstatBSseqBSseqStatBSseqTstatchrSelectBSseqcollapseBSseqcombinecombineListdata.frame2GRangesdmrFinderendend<-findLocifindOverlapsfisherTestsgetBSseqgetCoveragegetMethgetStatsgrangeshasBeenSmoothedncolnroworderBSseqoverlapsAnypDatapData<-plotManyRegionsplotRegionpoissonGoodnessOfFitread.bismarkread.modbam2bedread.modkitsampleNamessampleNames<-seqlengthsseqlengths<-seqlevelsseqlevels<-seqnamesseqnames<-showstartstart<-strandstrand<-strandCollapsesubsetByOverlapsupdateObjectwidthwidth<-

Dependencies:abindaskpassassortheadbeachmatBHBiobaseBiocGenericsBiocIOBiocParallelBiostringsbitopsBSgenomeclicodetoolscolorspacecpp11crayoncurldata.tableDelayedArrayDelayedMatrixStatsfarverformatRfutile.loggerfutile.optionsgenericsGenomeInfoDbGenomeInfoDbDataGenomicAlignmentsGenomicRangesgluegtoolsHDF5ArrayhttrIRangesjsonlitelabelinglambda.rlatticelifecyclelimmalocfitMatrixMatrixGenericsmatrixStatsmimemunsellopensslpermuteR.methodsS3R.ooR.utilsR6RColorBrewerRcppRCurlrestfulrrhdf5rhdf5filtersRhdf5libRhtslibrjsonrlangRsamtoolsrtracklayerS4ArraysS4VectorsscalessnowSparseArraysparseMatrixStatsstatmodSummarizedExperimentsysUCSC.utilsviridisLiteXMLXVectoryamlzlibbioc

Analyzing WGBS data with bsseq

Rendered frombsseq_analysis.Rmdusingknitr::rmarkdownon Dec 17 2024.

Last update: 2018-09-17
Started: 2017-10-17

The bsseq User's Guide

Rendered frombsseq.Rmdusingknitr::rmarkdownon Dec 17 2024.

Last update: 2018-09-17
Started: 2017-10-17

Readme and manuals

Help Manual

Help pageTopics
Whole-genome bisulfite sequencing for chromosome 22 from Lister et al.BS.chr22
BSmooth, smoothing bisulfite sequence dataBSmooth
Compute t-statistics based on smoothed whole-genome bisulfite sequencing data.BSmooth.tstat
The constructor function for BSseq objects.BSseq
Class BSseqassayNames,BSseq-method assays,BSseq-method BSseq-class chrSelectBSseq collapseBSseq combine,BSseq,BSseq-method combineList getBSseq hasBeenSmoothed length,BSseq-method orderBSseq pData,BSseq-method pData<-,BSseq,data.frame-method pData<-,BSseq,DataFrame-method sampleNames,BSseq-method sampleNames<-,BSseq,ANY-method show,BSseq-method strandCollapse updateObject,BSseq-method [,BSseq-method
Class BSseqStatBSseqStat BSseqStat-class show,BSseqStat-method updateObject,BSseqStat-method [,BSseqStat,ANY,ANY,ANY-method [,BSseqStat-method
Class BSseqTstatBSseqTstat BSseqTstat-class show,BSseqTstat-method updateObject,BSseqTstat-method [,BSseqTstat,ANY,ANY,ANY-method [,BSseqTstat-method
Converts a data frame to a GRanges.data.frame2GRanges
Finds differentially methylated regions for whole genome bisulfite sequencing data.dmrFinder
Find methylation loci in a genomefindLoci
Compute Fisher-tests for a BSseq objectfisherTests
Classes FWIRanges and FWGRangesdimnames,arrayRealizationSink-method end,FWGRanges-method end,FWIRanges-method end<-,FWIRanges-method findOverlaps,FWGRanges,FWGRanges-method FWGRanges-class FWIRanges-class names,FWIRanges-method names<-,FWIRanges-method seqnames,FWGRanges-method start,FWGRanges-method start,FWIRanges-method start<-,FWIRanges-method strand,FWGRanges-method width,FWGRanges-method width,FWIRanges-method width<-,FWIRanges-method
Obtain coverage for BSseq objects.getCoverage
Obtain methylation estimates for BSseq objects.getMeth
Obtain statistics from a BSseqTstat objectgetStats
Binomial and poisson goodness of fit statistics for BSSeq objectsbinomialGoodnessOfFit chisqGoodnessOfFit plot.chisqGoodnessOfFit poissonGoodnessOfFit print.chisqGoodnessOfFit
Class hasGRangesclass:hasGRanges end,hasGRanges-method end<-,hasGRanges-method findOverlaps,GenomicRanges,hasGRanges-method findOverlaps,hasGRanges,GenomicRanges-method findOverlaps,hasGRanges,hasGRanges-method granges,hasGRanges-method hasGRanges hasGRanges-class length,hasGRanges-method overlapsAny,GenomicRanges,hasGRanges-method overlapsAny,hasGRanges,GenomicRanges-method overlapsAny,hasGRanges,hasGRanges-method seqlengths,hasGRanges-method seqlengths<-,hasGRanges-method seqlevels,hasGRanges-method seqlevels<-,hasGRanges-method seqnames,hasGRanges-method seqnames<-,hasGRanges-method start,hasGRanges-method start<-,hasGRanges-method strand,hasGRanges-method strand<-,hasGRanges,ANY-method strand<-,hasGRanges-method subsetByOverlaps,GenomicRanges,hasGRanges-method subsetByOverlaps,hasGRanges,GenomicRanges-method subsetByOverlaps,hasGRanges,hasGRanges-method width,hasGRanges-method width<-,hasGRanges-method [,hasGRanges,ANY,ANY,ANY-method [,hasGRanges-method
Plotting BSmooth methylation estimatesplotManyRegions plotRegion
Parsing output from the Bismark alignment suite.read.bismark
Construct BSseq objects from nanopore BED filesread.modbam2bed
Construct BSseq objects from nanopore BED filesread.modkit