1. Introduction | 2. Installation | 3. Data Structure | 4. From raw_data to norm_data | 4.1 Data Preprocessing | 4.2 Data Analysis | 4.2.1 Individual Well Application | 4.2.2 96-Well Plate Application | 4.3 Data Summary | 5. From norm_data to tsar_data | 5.1 Merge Replicates | 5.1.1 Jumpstart to Graph | 5.2 Graphic Analysis | 5.2.1 Tm Boxplot | 5.2.2 TSA Curve Visualization | 5.2.3 Curves by Condition | 5.2.4 First Derivative Comparison | 6. Session Info | 6.1 Citation | 6.2 Session Info
Package: TSAR 1.11.0
Xinlin Gao
TSAR: Thermal Shift Analysis in R
This package automates analysis workflow for Thermal Shift Analysis (TSA) data. Processing, analyzing, and visualizing data through both shiny applications and command lines. Package aims to simplify data analysis and offer front to end workflow, from raw data to multiple trial analysis.
Authors:
TSAR_1.11.0.tar.gz
TSAR_1.11.0.zip(r-4.7)TSAR_1.11.0.zip(r-4.6)TSAR_1.11.0.zip(r-4.5)
TSAR_1.11.0.tgz(r-4.6-any)TSAR_1.11.0.tgz(r-4.5-any)
TSAR_1.11.0.tar.gz(r-4.7-any)TSAR_1.11.0.tar.gz(r-4.6-any)
TSAR_1.11.0.tgz(r-4.6-emscripten)
manual.pdf |manual.html✨
DESCRIPTION |NEWS
card.svg |card.png
TSAR/json (API)
| # Install 'TSAR' in R: |
| install.packages('TSAR', repos = c('https://bioc.r-universe.dev', 'https://cloud.r-project.org')) |
Datasets:
- example_normalized_data - Example normalized TSA dataset
- example_tsar_data - Example tsar_data file
- qPCR_data1 - QPCR_data1 Dataset
- qPCR_data2 - QPCR_data2 Dataset
- well_information - Example well information Data
- well_information_template - Well Information Template
On BioConductor:TSAR-1.11.0(bioc 3.24)TSAR-1.10.0(bioc 3.23)
This package does not link to any Github/Gitlab/R-forge repository. No issue tracker or development information is available.
softwareshinyappsvisualizationqpcr
Last updated from:5ead14fb1d. Checks:1 ERROR, 7 NOTE, 2 OK. Indexed: yes.
| Target | Result | Time | Files | Syslog |
|---|---|---|---|---|
| bioc-checks | ERROR | 222 | ||
| linux-devel-x86_64 | NOTE | 262 | ||
| source / vignettes | OK | 246 | ||
| linux-release-x86_64 | NOTE | 234 | ||
| macos-release-arm64 | NOTE | 134 | ||
| macos-oldrel-arm64 | NOTE | 123 | ||
| windows-devel | NOTE | 170 | ||
| windows-release | NOTE | 173 | ||
| windows-oldrel | NOTE | 166 | ||
| wasm-release | OK | 188 |
Exports:analyze_normcondition_IDsgam_analysisget_legendgraph_tsarjoin_well_infomerge_normmerge_TSAmodel_betamodel_boltzmannmodel_fitmodel_gamnormalizenormalize_fluorescenceread_analysisread_raw_dataread_tsarremove_rawrescalerun_boltzmannscreenTm_differenceTm_estTSA_averageTSA_boxplotTSA_compare_plotTSA_ligandsTSA_proteinsTSA_TmsTSA_wells_plotview_derivview_modelweed_rawwell_IDswrite_tsar
Dependencies:abindaskpassbackportsbase64encbootbroombslibcachemcarcarDatacellrangerclicolorspacecommonmarkcorrplotcowplotcpp11crayoncrosstalkcurldata.tableDerivdigestdoBydplyrevaluatefarverfastmapfontawesomeforecastFormulafracdifffsgenericsggplot2ggpubrggrepelggsciggsignifgluegridExtragtablehighrhmshtmltoolshtmlwidgetshttpuvhttrisobandjquerylibjsonliteknitrlabelinglaterlatticelazyevallifecyclelme4lmtestmagrittrMASSMatrixMatrixModelsmemoisemgcvmimeminpack.lmminqamodelrnlmenloptrnnetnumDerivopensslopenxlsxotelpbkrtestpillarpkgconfigplotlypolynomprettyunitsprogresspromisespurrrquantregR6rappdirsrbibutilsRColorBrewerRcppRcppArmadilloRcppEigenRdpackreadxlreformulasrematchrhandsontablerlangrmarkdownrstatixS7sassscalesshinyshinyjsshinyWidgetssourcetoolsSparseMstringistringrsurvivalsystibbletidyrtidyselecttimeDatetinytexurcautf8vctrsviridisLitewithrxfunxtableyamlzipzoo
TSAR Package Structure
Last update: 2025-12-19
Started: 2023-06-23
TSAR Workflow by Command
1. Introduction | 2. Installation | 3. Load Data | 4. Data Pre-Processing | 5. 96-well Analysis Application | 6. Intermediate Data Output | 7. Complete Dataset with Ligand and Protein Information | 8. Merge Data across Biological Replicates | 9. Tm Estimation Shift Visualization | 10. TSA Curve Visualization | 11. Session Info | 11.1 Citation | 11.2 Session Info
Last update: 2025-12-19
Started: 2023-07-05
TSAR Workflow by Shiny
1. Introduction | 2. Installation | 3. Load TSAR Package | 4. Data Pre-Processing | 5. Data Analysis | 6. Data Visualization | 7. Session Info | 7.1 Citation | 7.2 Session Info
Last update: 2025-12-15
Started: 2023-07-05
Frequently Asked Questions
FAQ | Verify Data Input | Locating Appropriate Function Calls | Under Data Pre-Processing, weed_raw(): | Under Data Analysis, analyze_norm(): | Under Data Visualization, graph_tsar(): | Session Info
Last update: 2024-04-06
Started: 2023-08-08
