Package: SpatialDecon 1.17.0

Maddy Griswold

SpatialDecon: Deconvolution of mixed cells from spatial and/or bulk gene expression data

Using spatial or bulk gene expression data, estimates abundance of mixed cell types within each observation. Based on "Advances in mixed cell deconvolution enable quantification of cell types in spatial transcriptomic data", Danaher (2022). Designed for use with the NanoString GeoMx platform, but applicable to any gene expression data.

Authors:Maddy Griswold [cre, aut], Patrick Danaher [aut]

SpatialDecon_1.17.0.tar.gz
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SpatialDecon_1.17.0.tgz(r-4.4-any)SpatialDecon_1.17.0.tgz(r-4.3-any)
SpatialDecon_1.17.0.tar.gz(r-4.5-noble)SpatialDecon_1.17.0.tar.gz(r-4.4-noble)
SpatialDecon_1.17.0.tgz(r-4.4-emscripten)
SpatialDecon.pdf |SpatialDecon.html
SpatialDecon/json (API)
NEWS

# Install 'SpatialDecon' in R:
install.packages('SpatialDecon', repos = c('https://bioc.r-universe.dev', 'https://cloud.r-project.org'))

Peer review:

Bug tracker:https://github.com/nanostring-biostats/spatialdecon/issues

Datasets:

On BioConductor:SpatialDecon-1.17.0(bioc 3.21)SpatialDecon-1.16.0(bioc 3.20)

immunooncologyfeatureextractiongeneexpressiontranscriptomicsspatial

7.75 score 33 stars 57 scripts 388 downloads 14 exports 125 dependencies

Last updated 2 months agofrom:7d7d8396ee. Checks:OK: 1 NOTE: 6. Indexed: yes.

TargetResultDate
Doc / VignettesOKNov 23 2024
R-4.5-winNOTENov 23 2024
R-4.5-linuxNOTENov 23 2024
R-4.4-winNOTENov 23 2024
R-4.4-macNOTENov 23 2024
R-4.3-winNOTENov 23 2024
R-4.3-macNOTENov 23 2024

Exports:collapseCellTypescreate_profile_matrixderive_GeoMx_backgrounddownload_profile_matrixfloretsmergeTumorIntoXreverseDeconrunCollapseCellTypesrunErrorModelrunMergeTumorIntoXrunReverseDeconrunspatialdeconspatialdeconTIL_barplot

Dependencies:askpassbase64encbeeswarmBiobaseBiocGenericsBiostringsbootbslibcachemcellrangerclicodetoolscolorspacecpp11crayoncurldata.tabledigestdotCall64dplyrEnvStatsevaluatefansifarverfastmapfontawesomeforcatsfsfuturefuture.applygenericsGenomeInfoDbGenomeInfoDbDataGeomxToolsGGallyggbeeswarmggiraphggplot2ggstatsggthemesglobalsgluegtablehighrhmshtmltoolshtmlwidgetshttrIRangesisobandjquerylibjsonliteknitrlabelinglatticelifecyclelistenvlme4lmerTestlogNormRegmagrittrMASSMatrixmemoisemgcvmimeminqamunsellNanoStringNCToolsnlmenloptrnortestnumDerivopensslparallellypatchworkpheatmappillarpkgconfigplyrprettyunitsprogressprogressrpurrrR.cacheR.methodsS3R.ooR.utilsR6rappdirsRColorBrewerRcppRcppEigenreadxlrematchrepmisreshape2rjsonrlangrmarkdownS4VectorssassscalesSeuratObjectspspamstringistringrsyssystemfontstibbletidyrtidyselecttinytexUCSC.utilsutf8uuidvctrsviporviridisLitewithrxfunXVectoryamlzlibbioc

Use of SpatialDecon in a large GeoMx dataset with GeomxTools

Rendered fromSpatialDecon_vignette_NSCLC.Rmdusingknitr::rmarkdownon Nov 23 2024.

Last update: 2023-10-30
Started: 2021-10-13

Use of SpatialDecon in a small GeoMx dataset

Rendered fromSpatialDecon_vignette.Rmdusingknitr::rmarkdownon Nov 23 2024.

Last update: 2024-01-11
Started: 2020-07-17

Readme and manuals

Help Manual

Help pageTopics
SpatialDecon: A package for estimating mixed cell type abundance in the regions of spatially-resolved gene expression studiesSpatialDecon-package
Default colors for the cell types in the safeTME matrixcellcols
Collapse related cell types within a deconvolution resultcollapseCellTypes
Create Custom Cell Profile Matrixcreate_profile_matrix
Derive background at the scale of the normalized data for GeoMx dataderive_GeoMx_background
Download a cell profile matrixdownload_profile_matrix
Draw coxcomb plots as points in a graphics windowflorets
Genes' biological variability in immune deconvolution from TCGA.mean.resid.sd
Estimate a tumor-specific profile and merge it with the pre-specified cell profile matrix (X)mergeTumorIntoX
Small example GeoMx datamini_geomx_dataset
Mini human colon single cell datasetmini_singleCell_dataset
Large example GeoMx datansclc
Reverse deconvolutionreverseDecon
Run collapseCellTypesrunCollapseCellTypes runCollapseCellTypes,NanoStringGeoMxSet-method
Apply error model to estimate technical SD from raw countsrunErrorModel
Run MergeTumorIntoXrunMergeTumorIntoX runMergeTumorIntoX,NanoStringGeoMxSet-method
Run ReversedeconrunReverseDecon runReverseDecon,NanoStringGeoMxSet-method
Run spatialdeconrunspatialdecon runspatialdecon,NanoStringGeoMxSet-method runspatialdecon,Seurat-method
SafeTME matrixsafeTME
Mapping from granularly-defined cell populations to broaded cell populationssafeTME.matches
Mixed cell deconvolution of spatiall-resolved gene expression dataspatialdecon
Barplot of abundance estimatesTIL_barplot