Package: PoDCall 1.13.0

Hans Petter Brodal

PoDCall: Positive Droplet Calling for DNA Methylation Droplet Digital PCR

Reads files exported from 'QX Manager or QuantaSoft' containing amplitude values from a run of ddPCR (96 well plate) and robustly sets thresholds to determine positive droplets for each channel of each individual well. Concentration and normalized concentration in addition to other metrics is then calculated for each well. Results are returned as a table, optionally written to file, as well as optional plots (scatterplot and histogram) for both channels per well written to file. The package includes a shiny application which provides an interactive and user-friendly interface to the full functionality of PoDCall.

Authors:Hans Petter Brodal [aut, cre], Marine Jeanmougin [aut], Guro Elisabeth Lind [aut]

PoDCall_1.13.0.tar.gz
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PoDCall.pdf |PoDCall.html
PoDCall/json (API)
NEWS

# Install 'PoDCall' in R:
install.packages('PoDCall', repos = c('https://bioc.r-universe.dev', 'https://cloud.r-project.org'))

Peer review:

Datasets:
  • thrTable - PoDCall Example Threshold Table

On BioConductor:PoDCall-1.13.0(bioc 3.20)PoDCall-1.12.0(bioc 3.19)

This package does not link to any Github/Gitlab/R-forge repository. No issue tracker or development information is available.

bioconductor-package

9 exports 0.61 score 82 dependencies 1 mentions

Last updated 2 months agofrom:febd185cd0

Exports:importAmplitudeDataimportSampleSheetpodcallChannelPlotpodcallDdpcrpodcallHistogrampodcallMultiplotpodcallScatterplotpodcallShinypodcallThresholds

Dependencies:base64encbitbit64bslibcachemclicliprcolorspacecommonmarkcpp11crayoncrosstalkdata.tabledigestdiptestDTevaluatefansifarverfastmapfontawesomefsggplot2gluegridExtragtablehighrhmshtmltoolshtmlwidgetshttpuvisobandjquerylibjsonliteknitrlabelingLaplacesDemonlaterlatticelazyevallifecyclemagrittrMASSMatrixmclustmemoisemgcvmimemunsellnlmepillarpkgconfigprettyunitsprogresspromisespurrrR6rappdirsRColorBrewerRcppreadrrlangrlistrmarkdownsassscalesshinyshinyjssourcetoolstibbletidyselecttinytextzdbutf8vctrsviridisLitevroomwithrxfunXMLxtableyaml

PoDCall: Positive Droplet Caller for DNA Methylation ddPCR

Rendered fromPoDCall.Rmdusingknitr::rmarkdownon Jun 17 2024.

Last update: 2023-12-20
Started: 2020-10-23

Readme and manuals

Help Manual

Help pageTopics
importAmplitudeDataimportAmplitudeData
importSampleSheetimportSampleSheet
podcallChannelPlotpodcallChannelPlot
Positive Droplet Calling for ddPCRpodcallDdpcr
podcallHistogrampodcallHistogram
podcallMultiplotpodcallMultiplot
podcallScatterplotpodcallScatterplot
PoDCall shiny launcherpodcallShiny
podcallThresholdspodcallThresholds
PoDCall Example Threshold TablethrTable