Package: PoDCall 1.15.0

Hans Petter Brodal

PoDCall: Positive Droplet Calling for DNA Methylation Droplet Digital PCR

Reads files exported from 'QX Manager or QuantaSoft' containing amplitude values from a run of ddPCR (96 well plate) and robustly sets thresholds to determine positive droplets for each channel of each individual well. Concentration and normalized concentration in addition to other metrics is then calculated for each well. Results are returned as a table, optionally written to file, as well as optional plots (scatterplot and histogram) for both channels per well written to file. The package includes a shiny application which provides an interactive and user-friendly interface to the full functionality of PoDCall.

Authors:Hans Petter Brodal [aut, cre], Marine Jeanmougin [aut], Guro Elisabeth Lind [aut]

PoDCall_1.15.0.tar.gz
PoDCall_1.15.0.zip(r-4.5)PoDCall_1.15.0.zip(r-4.4)PoDCall_1.13.6.zip(r-4.3)
PoDCall_1.15.0.tgz(r-4.4-any)PoDCall_1.13.6.tgz(r-4.3-any)
PoDCall_1.15.0.tar.gz(r-4.5-noble)PoDCall_1.15.0.tar.gz(r-4.4-noble)
PoDCall_1.15.0.tgz(r-4.4-emscripten)PoDCall_1.13.6.tgz(r-4.3-emscripten)
PoDCall.pdf |PoDCall.html
PoDCall/json (API)
NEWS

# Install 'PoDCall' in R:
install.packages('PoDCall', repos = c('https://bioc.r-universe.dev', 'https://cloud.r-project.org'))

Peer review:

Datasets:
  • thrTable - PoDCall Example Threshold Table

On BioConductor:PoDCall-1.15.0(bioc 3.21)PoDCall-1.14.0(bioc 3.20)

This package does not link to any Github/Gitlab/R-forge repository. No issue tracker or development information is available.

classificationepigeneticsddpcrdifferentialmethylationcpgislanddnamethylation

4.30 score 5 scripts 116 downloads 1 mentions 9 exports 82 dependencies

Last updated 21 days agofrom:8dec98c293. Checks:OK: 1 NOTE: 6. Indexed: yes.

TargetResultDate
Doc / VignettesOKOct 31 2024
R-4.5-winNOTEOct 31 2024
R-4.5-linuxNOTEOct 31 2024
R-4.4-winNOTEOct 31 2024
R-4.4-macNOTEOct 31 2024
R-4.3-winNOTESep 24 2024
R-4.3-macNOTESep 24 2024

Exports:importAmplitudeDataimportSampleSheetpodcallChannelPlotpodcallDdpcrpodcallHistogrampodcallMultiplotpodcallScatterplotpodcallShinypodcallThresholds

Dependencies:base64encbitbit64bslibcachemclicliprcolorspacecommonmarkcpp11crayoncrosstalkdata.tabledigestdiptestDTevaluatefansifarverfastmapfontawesomefsggplot2gluegridExtragtablehighrhmshtmltoolshtmlwidgetshttpuvisobandjquerylibjsonliteknitrlabelingLaplacesDemonlaterlatticelazyevallifecyclemagrittrMASSMatrixmclustmemoisemgcvmimemunsellnlmepillarpkgconfigprettyunitsprogresspromisespurrrR6rappdirsRColorBrewerRcppreadrrlangrlistrmarkdownsassscalesshinyshinyjssourcetoolstibbletidyselecttinytextzdbutf8vctrsviridisLitevroomwithrxfunXMLxtableyaml

PoDCall: Positive Droplet Caller for DNA Methylation ddPCR

Rendered fromPoDCall.Rmdusingknitr::rmarkdownon Oct 31 2024.

Last update: 2024-09-17
Started: 2020-10-23

Readme and manuals

Help Manual

Help pageTopics
importAmplitudeDataimportAmplitudeData
importSampleSheetimportSampleSheet
podcallChannelPlotpodcallChannelPlot
Positive Droplet Calling for ddPCRpodcallDdpcr
podcallHistogrampodcallHistogram
podcallMultiplotpodcallMultiplot
podcallScatterplotpodcallScatterplot
PoDCall shiny launcherpodcallShiny
podcallThresholdspodcallThresholds
PoDCall Example Threshold TablethrTable