Package: PoDCall 1.21.1

Hans Petter Brodal

PoDCall: Positive Droplet Calling for DNA Methylation Droplet Digital PCR

Reads files exported from 'QX Manager or QuantaSoft' containing amplitude values from a run of ddPCR (96 well plate) and robustly sets thresholds to determine positive droplets for each channel of each individual well. Concentration and normalized concentration in addition to other metrics is then calculated for each well. Results are returned as a table, optionally written to file, as well as optional plots (scatterplot and histogram) for both channels per well written to file. The package includes a shiny application which provides an interactive and user-friendly interface to the full functionality of PoDCall.

Authors:Hans Petter Brodal [aut, cre], Marine Jeanmougin [aut], Guro Elisabeth Lind [aut]

PoDCall_1.21.1.tar.gz
PoDCall_1.21.1.zip(r-4.7)PoDCall_1.21.1.zip(r-4.6)PoDCall_1.21.1.zip(r-4.5)
PoDCall_1.21.1.tgz(r-4.6-any)PoDCall_1.21.1.tgz(r-4.5-any)
PoDCall_1.21.1.tar.gz(r-4.7-any)PoDCall_1.21.1.tar.gz(r-4.6-any)
PoDCall_1.21.1.tgz(r-4.6-emscripten)
manual.pdf |manual.html
DESCRIPTION |NEWS
card.svg |card.png
PoDCall/json (API)

# Install 'PoDCall' in R:
install.packages('PoDCall', repos = c('https://bioc.r-universe.dev', 'https://cloud.r-project.org'))
Datasets:
  • thrTable - PoDCall Example Threshold Table

On BioConductor:PoDCall-1.21.0(bioc 3.24)PoDCall-1.20.0(bioc 3.23)

This package does not link to any Github/Gitlab/R-forge repository. No issue tracker or development information is available.

classificationepigeneticsddpcrdifferentialmethylationcpgislanddnamethylation

2.78 score 12 scripts 280 downloads 1 mentions 9 exports 78 dependencies

Last updated from:42f80025e8. Checks:1 WARNING, 8 ERROR, 1 OK. Indexed: yes.

TargetResultTimeFilesSyslog
bioc-checksWARNING174
linux-devel-x86_64ERROR600
source / vignettesERROR191
linux-release-x86_64ERROR738
macos-release-arm64ERROR275
macos-oldrel-arm64ERROR262
windows-develERROR484
windows-releaseERROR514
windows-oldrelERROR508
wasm-releaseOK132

Exports:importAmplitudeDataimportSampleSheetpodcallChannelPlotpodcallDdpcrpodcallHistogrampodcallMultiplotpodcallScatterplotpodcallShinypodcallThresholds

Dependencies:base64encbitbit64bslibcachemclicliprcommonmarkcpp11crayoncrosstalkdata.tabledigestdiptestdplyrDTevaluatefarverfastmapfontawesomefsgenericsggplot2gluegridExtragtablehighrhmshtmltoolshtmlwidgetshttpuvisobandjquerylibjsonliteknitrlabelingLaplacesDemonlaterlazyevallifecyclemagrittrmclustmemoisemimeotelpillarpkgconfigprettyunitsprogresspromisespurrrR6rappdirsRColorBrewerRcppreadrrlangrlistrmarkdownS7sassscalesshinyshinyjssourcetoolstibbletidyselecttinytextzdbutf8vctrsviridisLitevroomwithrxfunXMLxtableyaml

Readme and manuals

Help Manual

Help pageTopics
importAmplitudeDataimportAmplitudeData
importSampleSheetimportSampleSheet
podcallChannelPlotpodcallChannelPlot
Positive Droplet Calling for ddPCRpodcallDdpcr
podcallHistogrampodcallHistogram
podcallMultiplotpodcallMultiplot
podcallScatterplotpodcallScatterplot
PoDCall shiny launcherpodcallShiny
podcallThresholdspodcallThresholds
PoDCall Example Threshold TablethrTable