Package: NanoMethViz 3.3.0

Shian Su

NanoMethViz: Visualise methylation data from Oxford Nanopore sequencing

NanoMethViz is a toolkit for visualising methylation data from Oxford Nanopore sequencing. It can be used to explore methylation patterns from reads derived from Oxford Nanopore direct DNA sequencing with methylation called by callers including nanopolish, f5c and megalodon. The plots in this package allow the visualisation of methylation profiles aggregated over experimental groups and across classes of genomic features.

Authors:Shian Su [cre, aut]

NanoMethViz_3.3.0.tar.gz
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NanoMethViz.pdf |NanoMethViz.html
NanoMethViz/json (API)
NEWS

# Install 'NanoMethViz' in R:
install.packages('NanoMethViz', repos = c('https://bioc.r-universe.dev', 'https://cloud.r-project.org'))

Peer review:

Bug tracker:https://github.com/shians/nanomethviz/issues

Pkgdown:https://shians.github.io

Uses libs:
  • zlib– Compression library
  • c++– GNU Standard C++ Library v3

On BioConductor:NanoMethViz-3.3.0(bioc 3.21)NanoMethViz-3.2.0(bioc 3.20)

softwarelongreadvisualizationdifferentialmethylationdnamethylationepigeneticsdataimportzlibcpp

6.98 score 24 stars 12 scripts 260 downloads 52 exports 140 dependencies

Last updated 2 months agofrom:1ee21fe29e. Checks:OK: 9. Indexed: yes.

TargetResultDate
Doc / VignettesOKNov 25 2024
R-4.5-win-x86_64OKNov 25 2024
R-4.5-linux-x86_64OKNov 25 2024
R-4.4-win-x86_64OKNov 25 2024
R-4.4-mac-x86_64OKNov 25 2024
R-4.4-mac-aarch64OKNov 25 2024
R-4.3-win-x86_64OKNov 25 2024
R-4.3-mac-x86_64OKNov 25 2024
R-4.3-mac-aarch64OKNov 25 2024

Exports:bsseq_to_edgerbsseq_to_log_methy_ratiocluster_regionscreate_tabix_fileexonsexons_to_genesexons<-filter_methyget_cgi_grcm39get_cgi_hg19get_cgi_hg38get_cgi_mm10get_example_exons_mus_musculusget_exons_grcm39get_exons_hg19get_exons_hg38get_exons_homo_sapiensget_exons_mm10get_exons_mus_musculusload_example_modbamresultload_example_nanomethresultmethymethy_col_namesmethy_to_bsseqmethy_to_edgermethy<-mod_codemod_code<-modbam_to_tabixModBamFilesModBamResultNanoMethResultplot_agg_genesplot_agg_regionsplot_geneplot_gene_heatmapplot_grangeplot_grange_heatmapplot_mdsplot_pcaplot_regionplot_region_heatmapplot_violinquery_exons_gene_idquery_exons_regionquery_exons_symbolquery_methyregion_methy_statssamplessamples<-showsigmoid

Dependencies:abindAnnotationDbiaskpassassertthatassortheadbeachmatbeeswarmBHBiobaseBiocGenericsBiocIOBiocParallelBiocSingularBiostringsbitbit64bitopsblobBSgenomebsseqcachemCairoclassclicliprcodetoolscolorspacecpp11crayoncurldata.tableDBIdbscanDelayedArrayDelayedMatrixStatsdplyre1071fansifarverfastmapforcatsformatRfsfutile.loggerfutile.optionsgenericsGenomeInfoDbGenomeInfoDbDataGenomicAlignmentsGenomicRangesggbeeswarmggplot2ggrastrgluegtablegtoolsHDF5ArrayhmshttrIRangesirlbaisobandjsonliteKEGGRESTlabelinglambda.rlatticelifecyclelimmalocfitmagrittrMASSMatrixMatrixGenericsmatrixStatsmemoisemgcvmimemunsellnlmeopensslpatchworkpermutepillarpkgconfigplogrpngprettyunitsprogressproxypurrrR.methodsS3R.ooR.utilsR6raggRColorBrewerRcppRCurlreadrrestfulrrhdf5rhdf5filtersRhdf5libRhtslibrjsonrlangRsamtoolsRSQLitersvdrtracklayerS4ArraysS4VectorsScaledMatrixscalessnowSparseArraysparseMatrixStatsstatmodstringistringrSummarizedExperimentsyssystemfontstextshapingtibbletidyrtidyselecttzdbUCSC.utilsutf8vctrsviporviridisLitevroomwithrXMLXVectoryamlzlibbioc

User's Guide

Rendered fromUsersGuide.Rmdusingknitr::rmarkdownon Nov 25 2024.

Last update: 2024-05-21
Started: 2023-10-17

Readme and manuals

Help Manual

Help pageTopics
Convert BSseq object to edgeR methylation matrixbsseq_to_edger
Convert BSseq object to log-methylation-ratio matrixbsseq_to_log_methy_ratio
Cluster reads based on methylationcluster_reads
Cluster regions by K-meanscluster_regions
Create a tabix file using methylation callscreate_tabix_file
Convert exon annotation to genesexons_to_genes
Create filtered methylation filefilter_methy
Get exon annotationsget_cgi_grcm39 get_cgi_hg19 get_cgi_hg38 get_cgi_mm10 get_exons get_exons_grcm39 get_exons_hg19 get_exons_hg38 get_exons_mm10
Get example exon annotations for mus musculus (mm10)get_example_exons_mus_musculus
Get exon annotations for Homo sapiens (hg19)get_exons_homo_sapiens
Get exon annotations for Mus musculus (mm10)get_exons_mus_musculus
Load an example ModBamResult objectload_example_modbamresult
Load an example NanoMethResult objectload_example_nanomethresult
Column names for methylation datamethy_col_names
Create BSSeq object from methylation tabix filemethy_to_bsseq
Convert NanoMethResult object to edgeR methylation matrixmethy_to_edger
Convert BAM with modifications to tabix formatmodbam_to_tabix
Constructor for a ModBamFiles objectModBamFiles show,ModBamFiles-method
ModBamFiles classModBamFiles-class
Modbam methylation resultsexons,ModBamResult-method exons<-,ModBamResult,data.frame-method methy,ModBamResult-method methy<-,ModBamResult,ModBamFiles-method ModBamResult ModBamResult-class mod_code,ModBamResult-method mod_code<-,ModBamResult,character-method samples,ModBamResult-method samples<-,ModBamResult,data.frame-method
Nanopore Methylation Resultexons,NanoMethResult-method exons<-,NanoMethResult,data.frame-method methy,NanoMethResult-method methy<-,NanoMethResult,ANY-method NanoMethResult NanoMethResult-class samples,NanoMethResult-method samples<-,NanoMethResult,data.frame-method
Plot gene aggregate plotplot_agg_genes
Plot aggregate regionsplot_agg_regions
Plot gene methylationplot_gene plot_gene,ModBamResult,character-method plot_gene,NanoMethResult,character-method
Plot gene methylation heatmapplot_gene_heatmap plot_gene_heatmap,ModBamResult,character-method plot_gene_heatmap,NanoMethResult,character-method
Plot GRangesplot_grange
Plot GRanges heatmapplot_grange_heatmap
Plot MDSplot_mds
Plot PCAplot_pca
Plot region methylationplot_region plot_region,ModBamResult,character,numeric,numeric-method plot_region,ModBamResult,factor,numeric,numeric-method plot_region,NanoMethResult,character,numeric,numeric-method plot_region,NanoMethResult,factor,numeric,numeric-method
Plot region methylation heatmapplot_region_heatmap plot_region_heatmap,ModBamResult,character,numeric,numeric-method plot_region_heatmap,ModBamResult,factor,numeric,numeric-method plot_region_heatmap,NanoMethResult,character,numeric,numeric-method plot_region_heatmap,NanoMethResult,factor,numeric,numeric-method
Plot violin for regionsplot_violin
Query exonsquery_exons query_exons_gene_id query_exons_region query_exons_symbol
Query methylation dataquery_methy
Calculate region methylation statisticsregion_methy_stats