Package: ASpli 2.17.0

Ariel Chernomoretz

ASpli: Analysis of Alternative Splicing Using RNA-Seq

Integrative pipeline for the analysis of alternative splicing using RNAseq.

Authors:Estefania Mancini, Andres Rabinovich, Javier Iserte, Marcelo Yanovsky and Ariel Chernomoretz

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ASpli.pdf |ASpli.html
ASpli/json (API)
NEWS

# Install 'ASpli' in R:
install.packages('ASpli', repos = c('https://bioc.r-universe.dev', 'https://cloud.r-project.org'))

Peer review:

On BioConductor:ASpli-2.15.0(bioc 3.20)ASpli-2.14.0(bioc 3.19)

This package does not link to any Github/Gitlab/R-forge repository. No issue tracker or development information is available.

immunooncologygeneexpressiontranscriptionalternativesplicingcoveragedifferentialexpressiondifferentialsplicingtimecoursernaseqgenomeannotationsequencingalignment

4.73 score 1 packages 45 scripts 344 downloads 113 exports 164 dependencies

Last updated 23 days agofrom:5b28a12bfb. Checks:OK: 1 WARNING: 6. Indexed: yes.

TargetResultDate
Doc / VignettesOKOct 30 2024
R-4.5-winWARNINGOct 30 2024
R-4.5-linuxWARNINGOct 30 2024
R-4.4-winWARNINGOct 30 2024
R-4.4-macWARNINGOct 30 2024
R-4.3-winWARNINGOct 30 2024
R-4.3-macWARNINGOct 30 2024

Exports:altPSIaltPSI<-anchorbasedanchorbased<-anchorcanchorc<-anchorjanchorj<-AsDiscoveraspliASexampleaspliBamsExampleaspliCountsExampleaspliDUexample1aspliDUexample2aspliExampleBamListaspliExampleGTFaspliFeaturesExampleaspliJunctionDUexampleaspliTargetsExamplebinbasedbinbased<-binGenomebinsDUbinsDU<-condition.ordercontainsGenesAndBinscontainsJunctionscountsbcountsb<-countse1icountse1i<-countsgcountsg<-countsie2countsie2<-countsjcountsj<-DUreportDUreport.normDUreport.offsetDUreportBinSpliceesPSIesPSI<-exportIntegratedSignalsexportSplicingReportsfeaturesbfeaturesb<-featuresgfeaturesg<-featuresjfeaturesj<-filterDUfiltersfilters<-filterSignalsgbCountsgbDUreportgenesDEgenesDE<-getConditionsintegrateSignalsirPIRirPIR<-jaltjalt<-jCountsjDUreportjesjes<-jirjir<-jointjoint<-junctionDUreportjunctionsDUjunctionsDU<-junctionsPIRjunctionsPIR<-junctionsPJUjunctionsPJU<-loadBAMlocalebasedlocalebased<-localeclocalec<-localejlocalej<-mergeBinDUASplotBinsplotGenomicRegionsrdsrdsbrdsb<-rdsgrdsg<-readCountsshowsignalssignals<-splicingReportsubsetsubsetBamssubsetTargetstargetstranscriptExonstranscriptExons<-writeAllwriteASwriteCountswriteDUwriteJDUwriteRdswriteSplicingReport

Dependencies:abindAnnotationDbiAnnotationFilteraskpassbackportsbase64encBHBiobaseBiocFileCacheBiocGenericsBiocIOBiocManagerBiocParallelBiocStylebiomaRtBiostringsbiovizBasebitbit64bitopsblobbookdownBSgenomebslibcachemcheckmatecliclustercodetoolscolorspacecpp11crayoncrosstalkcurldata.tableDBIdbplyrDelayedArraydeldirdichromatdigestdplyrDTedgeRensembldbevaluatefansifarverfastmapfilelockfontawesomeforeignformatRFormulafsfutile.loggerfutile.optionsgenericsGenomeInfoDbGenomeInfoDbDataGenomicAlignmentsGenomicFeaturesGenomicRangesggplot2gluegridExtragtableGvizhighrHmischmshtmlTablehtmltoolshtmlwidgetshttpuvhttrhttr2igraphinterpIRangesisobandjpegjquerylibjsonliteKEGGRESTknitrlabelinglambda.rlaterlatticelatticeExtralazyevallifecyclelimmalocfitmagrittrMASSMatrixMatrixGenericsmatrixStatsmemoisemgcvmimemunsellnlmennetopensslpbmcapplypillarpkgconfigplogrplyrpngprettyunitsprogresspromisesProtGenericspurrrR6rappdirsRColorBrewerRcppRcppEigenRCurlrestfulrRhtslibrjsonrlangrmarkdownrpartRsamtoolsRSQLiterstudioapirtracklayerS4ArraysS4VectorssassscalessnowSparseArraystatmodstringistringrSummarizedExperimentsystibbletidyrtidyselecttinytextxdbmakerUCSC.utilsUpSetRutf8VariantAnnotationvctrsviridisviridisLitewithrxfunXMLxml2XVectoryamlzlibbioc

ASpli

Rendered fromASpli.Rnwusingutils::Sweaveon Oct 30 2024.

Last update: 2024-03-20
Started: 2023-09-05

Readme and manuals

Help Manual

Help pageTopics
Analysis of Alternative Splicing Using RNAseqASpli-package ASpli
Accessors for ASpliAS objectaltPSI altPSI<- esPSI esPSI<- irPIR irPIR<- joint joint<- junctionsPIR junctionsPIR<- junctionsPJU junctionsPJU<-
Deprecated functions in package 'ASpli'ASpli-deprecated
Class '"ASpliAS"'altPSI,ASpliAS-method altPSI<-,ASpliAS,data.frame-method ASpliAS ASpliAS-class esPSI,ASpliAS-method esPSI<-,ASpliAS,data.frame-method irPIR,ASpliAS-method irPIR<-,ASpliAS,data.frame-method jDUreport,ASpliAS-method joint,ASpliAS-method joint<-,ASpliAS,data.frame-method junctionsPIR,ASpliAS-method junctionsPIR<-,ASpliAS,data.frame-method junctionsPJU,ASpliAS-method junctionsPJU<-,ASpliAS,data.frame-method subset,ASpliAS-method writeAS,ASpliAS-method
Class '"ASpliCounts"'ASpliCounts
Class '"ASpliCounts"'AsDiscover,ASpliCounts-method ASpliCounts-class condition.order,ASpliCounts-method countsb,ASpliCounts-method countsb<-,ASpliCounts,data.frame-method countse1i,ASpliCounts-method countse1i<-,ASpliCounts,data.frame-method countsg,ASpliCounts-method countsg<-,ASpliCounts,data.frame-method countsie2,ASpliCounts-method countsie2<-,ASpliCounts,data.frame-method countsj,ASpliCounts-method countsj<-,ASpliCounts,data.frame-method DUreport,ASpliCounts-method DUreport.norm,ASpliCounts-method DUreport.offset,ASpliCounts-method DUreportBinSplice,ASpliCounts-method gbDUreport,ASpliCounts-method jCounts,ASpliCounts-method junctionDUreport,ASpliCounts-method plotBins,ASpliCounts-method rds,ASpliCounts-method rdsb,ASpliCounts-method rdsb<-,ASpliCounts,data.frame-method rdsg,ASpliCounts-method rdsg<-,ASpliCounts,data.frame-method subset,ASpliCounts-method targets,ASpliCounts-method targets<-,ASpliCounts,data.frame-method writeAll,ASpliCounts-method writeCounts,ASpliCounts-method writeRds,ASpliCounts-method
Class '"ASpliDU"'ASpliDU ASpliDU-class binsDU,ASpliDU-method binsDU<-,ASpliDU-method containsGenesAndBins,ASpliDU-method containsJunctions,ASpliDU-method filterDU,ASpliDU-method genesDE,ASpliDU-method genesDE<-,ASpliDU,data.frame-method junctionsDU,ASpliDU-method junctionsDU<-,ASpliDU,data.frame-method mergeBinDUAS,ASpliDU,ASpliAS-method splicingReport,ASpliDU-method writeDU,ASpliDU-method
Class '"ASpliFeatures"'ASpliFeatures-class featuresb,ASpliFeatures-method featuresb<-,ASpliFeatures,GRanges-method featuresg,ASpliFeatures-method featuresg<-,ASpliFeatures,GRangesList-method featuresj,ASpliFeatures-method featuresj<-,ASpliFeatures,GRanges-method gbCounts,ASpliFeatures-method plotGenomicRegions,ASpliFeatures-method readCounts,ASpliFeatures-method transcriptExons,ASpliFeatures-method transcriptExons<-,ASpliFeatures,GRangesList-method
Class '"ASpliIntegratedSignals"'ASpliIntegratedSignals ASpliIntegratedSignals-class exportIntegratedSignals,ASpliIntegratedSignals-method filters,ASpliIntegratedSignals-method filters<-,ASpliIntegratedSignals,data.frame-method show,ASpliIntegratedSignals-method signals,ASpliIntegratedSignals-method signals<-,ASpliIntegratedSignals,data.frame-method splicingReport,ASpliIntegratedSignals-method writeIntegratedSignals,ASpliIntegratedSignals-method
Class '"ASpliJDU"'anchorc,ASpliJDU-method anchorc<-,ASpliJDU,data.frame-method anchorj,ASpliJDU-method anchorj<-,ASpliJDU,data.frame-method ASpliJDU ASpliJDU-class jalt,ASpliJDU-method jalt<-,ASpliJDU,data.frame-method jDUreport,ASpliJDU-method jes,ASpliJDU-method jes<-,ASpliJDU,data.frame-method jir,ASpliJDU-method jir<-,ASpliJDU,data.frame-method localec,ASpliJDU-method localec<-,ASpliJDU,data.frame-method localej,ASpliJDU-method localej<-,ASpliJDU,data.frame-method show,ASpliJDU-method writeJDU,ASpliJDU-method
Class '"ASpliSplicingReport"'anchorbased,ASpliSplicingReport-method anchorbased<-,ASpliSplicingReport,data.frame-method ASpliSplicingReport ASpliSplicingReport-class binbased,ASpliSplicingReport-method binbased<-,ASpliSplicingReport,data.frame-method exportSplicingReports,ASpliSplicingReport-method filterSignals,ASpliSplicingReport-method integrateSignals,ASpliSplicingReport-method localebased,ASpliSplicingReport-method localebased<-,ASpliSplicingReport,data.frame-method show,ASpliSplicingReport-method splicingReport,ASpliSplicingReport-method writeSplicingReport,ASpliSplicingReport-method
Feature coordinates extractionbinGenome
Feature coordinates extractionbinGenome,TxDb-method binGenome-methods
Accessors for ASpliCounts objectcondition.order countsb countsb<- countse1i countse1i<- countsg countsg<- countsie2 countsie2<- countsj countsj<- rdsb rdsb<- rdsg rdsg<- targets
Accessors for ASpliDU objectbinsDU binsDU<- genesDE genesDE<- junctionsDU junctionsDU<-
Differential gene expression and differential bin usage estimationDUreport
Differential gene expression and differential bin usage estimationDUreport.norm
Differential gene expression and differential bin usage estimationDUreport.offset
Differential gene expression and differential bin usage estimationDUreportBinSplice
Examine ASpliDU objectscontainsGenesAndBins containsJunctions
Example Aspli objectsaspliASexample aspliBamsExample aspliCountsExample aspliDUexample1 aspliDUexample2 aspliExampleBamList aspliExampleGTF aspliFeaturesExample aspliJunctionDUexample aspliTargetsExample
Export integrated signals.exportIntegratedSignals
Export splicing reportsexportSplicingReports
Accessors for ASpliFeatures objectfeaturesb featuresb<- featuresg featuresg<- featuresj featuresj<- transcriptExons transcriptExons<-
Filtering ASpliDU objectsfilterDU
Filter signalsfilterSignals
Summarize read overlapsgbCounts readCounts
Differential gene expression and differential bin usage estimationgbDUreport
Retrieve condition names from a targets data frame.getConditions
Accessors for ASpliIntegratedSignals objectfilters filters<- signals signals<-
Integrate signalsintegrateSignals
Report PSI, PJU and PIR using experimental junctionsAsDiscover jCounts
Accessors for ASpliJDU objectanchorc anchorc<- anchorj anchorj<- jalt jalt<- jes jes<- jir jir<- localec localec<- localej localej<-
Differential junction usage estimationjDUreport
Differential junction usage estimationjunctionDUreport
Load BAM filesloadBAM
Differential usage of bins and PSI/PIR.mergeBinDUAS
Draw plots of gene counts, bin counts, PSI/PIR value, inclusion and exclusion junctions for selected bins.plotBins
Create genomic regions coverage plotsplotGenomicRegions
Read density of gene and binsrds
Display a summary of data contained in ASpliObjectsshow,ASpliAS-method show,ASpliCounts-method show,ASpliDU-method show,ASpliFeatures-method show,ASpliMergedReports-method show-methods
Splicing reportsplicingReport
Accessors for ASpliSplicingReport objectanchorbased anchorbased<- binbased binbased<- localebased localebased<-
Subset ASpli objectssubset subsetBams subsetTargets
Write resultswriteAll writeAS writeCounts writeDU writeJDU writeRds writeSplicingReport
Write resultswriteAll,ANY-method writeAS-methods writeCounts-methods writeDU-methods writeJDU-methods writeRds-methods writeSplicingReport-methods