Package 'QFeatures'

Description

This function aggregates the quantitative features of an assay, applying a summarisation function (fun) to sets of features. The fcol variable name points to a rowData column that defines how to group the features during aggregate. This variable can eigher be a vector (we then refer to an aggregation by vector) or an adjacency matrix (aggregation by matrix).

The rowData of the aggregated SummarizedExperiment assay contains a .n variable that provides the number of parent features that were aggregated.

When aggregating with a vector, the newly aggregated SummarizedExperiment assay also contains a new aggcounts assay containing the aggregation counts matrix, i.e. the number of features that were aggregated for each sample, which can be accessed with the aggcounts() accessor.

Usage

## S4 method for signature 'QFeatures'
aggregateFeatures(
  object,
  i,
  fcol,
  name = "newAssay",
  fun = MsCoreUtils::robustSummary,
  ...
)

## S4 method for signature 'SummarizedExperiment'
aggregateFeatures(object, fcol, fun = MsCoreUtils::robustSummary, ...)

## S4 method for signature 'QFeatures'
adjacencyMatrix(object, i, adjName = "adjacencyMatrix")

adjacencyMatrix(object, i, adjName = "adjacencyMatrix") <- value

## S4 method for signature 'SummarizedExperiment'
aggcounts(object, ...)

Arguments

Details

Aggregation is performed by a function that takes a matrix as input and returns a vector of length equal to ncol(x). Examples thereof are

• MsCoreUtils::medianPolish() to fits an additive model (two way decomposition) using Tukey's median polish_ procedure using stats::medpolish();

• MsCoreUtils::robustSummary() to calculate a robust aggregation using MASS::rlm() (default);

• base::colMeans() to use the mean of each column;

• colMeansMat(x, MAT) to aggregate feature by the calculating the mean of peptide intensities via an adjacency matrix. Shared peptides are re-used multiple times.

• matrixStats::colMedians() to use the median of each column.

• base::colSums() to use the sum of each column;

• colSumsMat(x, MAT) to aggregate feature by the summing the peptide intensities for each protein via an adjacency matrix. Shared peptides are re-used multiple times.

See MsCoreUtils::aggregate_by_vector() for more aggregation functions.

Value

A QFeatures object with an additional assay or a SummarizedExperiment object (or subclass thereof).

Missing quantitative values

Missing quantitative values have different effects based on the aggregation method employed:

• The aggregation functions should be able to deal with missing values by either ignoring or propagating them. This is often done with an na.rm argument, that can be passed with .... For example, rowSums, rowMeans, rowMedians, ... will ignore NA values with na.rm = TRUE, as illustrated below.

• Missing values will result in an error when using medpolish, unless na.rm = TRUE is used. Note that this option relies on implicit assumptions and/or performes an implicit imputation: when summing, the values are implicitly imputed by 0, assuming that the NA represent a trully absent features; when averaging, the assumption is that the NA represented a genuinely missing value.

• When using robust summarisation, individual missing values are excluded prior to fitting the linear model by robust regression. To remove all values in the feature containing the missing values, use filterNA().

More generally, missing values often need dedicated handling such as filtering (see filterNA()) or imputation (see impute()).

Missing values in the row data

Missing values in the row data of an assay will also impact the resulting (aggregated) assay row data, as illustrated in the example below. Any feature variables (a column in the row data) containing NA values will be dropped from the aggregated row data. The reasons underlying this drop are detailed in the reduceDataFrame() manual page: only invariant aggregated rows, i.e. rows resulting from the aggregation from identical variables, are preserved during aggregations.

The situation illustrated below should however only happen in rare cases and should often be imputable using the value of the other aggregation rows before aggregation to preserve the invariant nature of that column. In cases where an NA is present in an otherwise variant column, the column would be dropped anyway.

Using an adjacency matrix

When considering non-unique peptides explicitly, i.e. peptides that map to multiple proteins rather than as a protein group, it is convenient to encode this ambiguity explicitly using a peptide-by-proteins (sparse) adjacency matrix. This matrix is typically stored in the rowdata and set/retrieved with the adjacencyMatrix() function. It can be created manually (as illustrated below) or using PSMatch::makeAdjacencyMatrix().

See Also

The QFeatures vignette provides an extended example and the Processing vignette, for a complete quantitative proteomics data processing pipeline. The MsCoreUtils::aggregate_by_vector() manual page provides further details.

Examples

## ---------------------------------------
## An example QFeatures with PSM-level data
## ---------------------------------------
data(feat1)
feat1

## Aggregate PSMs into peptides
feat1 <- aggregateFeatures(feat1, "psms", "Sequence", name = "peptides")
feat1

## Aggregate peptides into proteins
feat1 <- aggregateFeatures(feat1, "peptides", "Protein", name = "proteins")
feat1

assay(feat1[[1]])
assay(feat1[[2]])
aggcounts(feat1[[2]])
assay(feat1[[3]])
aggcounts(feat1[[3]])

## --------------------------------------------
## Aggregation with missing quantitative values
## --------------------------------------------
data(ft_na)
ft_na

assay(ft_na[[1]])
rowData(ft_na[[1]])

## By default, missing values are propagated
ft2 <- aggregateFeatures(ft_na, 1, fcol = "X", fun = colSums)
assay(ft2[[2]])
aggcounts(ft2[[2]])

## The rowData .n variable tallies number of initial rows that
## were aggregated (irrespective of NAs) for all the samples.
rowData(ft2[[2]])

## Ignored when setting na.rm = TRUE
ft3 <- aggregateFeatures(ft_na, 1, fcol = "X", fun = colSums, na.rm = TRUE)
assay(ft3[[2]])
aggcounts(ft3[[2]])

## -----------------------------------------------
## Aggregation with missing values in the row data
## -----------------------------------------------
## Row data results without any NAs, which includes the
## Y variables
rowData(ft2[[2]])

## Missing value in the Y feature variable
rowData(ft_na[[1]])[1, "Y"] <- NA
rowData(ft_na[[1]])

ft3 <- aggregateFeatures(ft_na, 1, fcol = "X", fun = colSums)
## The Y feature variable has been dropped!
assay(ft3[[2]])
rowData(ft3[[2]])

## --------------------------------------------
## Using a peptide-by-proteins adjacency matrix
## --------------------------------------------

## Let's use assay peptides from object feat1 and
## define that peptide SYGFNAAR maps to proteins
## Prot A and B

se <- feat1[["peptides"]]
rowData(se)$Protein[3] <- c("ProtA;ProtB")
rowData(se)

## This can also be defined using anadjacency matrix, manual
## encoding here. See PSMatch::makeAdjacencyMatrix() for a
## function that does it automatically.
adj <- matrix(0, nrow = 3, ncol = 2,
              dimnames = list(rownames(se),
                              c("ProtA", "ProtB")))
adj[1, 1] <- adj[2, 2] <- adj[3, 1:2] <- 1
adj

adjacencyMatrix(se) <- adj
rowData(se)
adjacencyMatrix(se)

## Aggregation using the adjacency matrix
se2 <- aggregateFeatures(se, fcol = "adjacencyMatrix",
                         fun = MsCoreUtils::colMeansMat)

## Peptide SYGFNAAR was taken into account in both ProtA and ProtB
## aggregations.
assay(se2)


## Aggregation by matrix on a QFeature object works as with a
## vector
ft <- QFeatures(list(peps = se))
ft <- aggregateFeatures(ft, "peps", "adjacencyMatrix", name = "protsByMat",
                        fun = MsCoreUtils::colMeansMat)
assay(ft[[2]])
rowData(ft[[2]])

Description

Placeholder for generics functions documentation

Description

Links between assays within a QFeatures object are handled by an AssayLinks object. It is composed by a list of AssayLink instances.

Usage

## S4 method for signature 'AssayLink'
show(object)

## S4 method for signature 'AssayLinks'
updateObject(object, ..., verbose = FALSE)

## S4 method for signature 'AssayLink'
updateObject(object, ..., verbose = FALSE)

AssayLink(name, from = NA_character_, fcol = NA_character_, hits = Hits())

AssayLinks(..., names = NULL)

assayLink(x, i)

assayLinks(x, i)

## S4 method for signature 'AssayLink,character,ANY,ANY'
x[i, j, ..., drop = TRUE]

## S4 method for signature 'AssayLinks,list,ANY,ANY'
x[i, j, ..., drop = TRUE]

addAssayLink(object, from, to, varFrom, varTo)

addAssayLinkOneToOne(object, from, to)

Arguments

Value

assayLink returns an instance of class AssayLink.

assayLinks returns an instance of class AssayLinks.

Constructors

Object can be created with the AssayLink() and AssayLinks() constructors.

Methods and functions

• assayLink(x, i) accesses the AssayLink at position i or with name i in the QFeatures object x.

• parentAssayLinks(x, i, recursive = FALSE) accesses the parent(s) AssayLinks or assay with index or name i.

Creating links between assays

• addAssayLink takes a parent assay and a child assay contained in the QFeatures object and creates a link given a matching feature variable in each assay's rowData. addAssayLink also allows to link an assay from multiple parent assays (see examples below).

• addAssayLinkOneToOne links two assays contained in the QFeatures object. The parent assay and the child assay must have the same size and contain the same rownames (a different ordering is allowed). The matching is performed based on the row names of the assays, instead of a supplied variable name in rowData. Providing multiple parents is not supported.

Examples

##-----------------------------
## Creating an AssayLink object
##-----------------------------

al1 <- AssayLink(name = "assay1")
al1

##------------------------------
## Creating an AssayLinks object
##------------------------------

AssayLinks(al1)

al2 <- AssayLinks(names = c("Assay1", "Assay2"))
al2

##---------------------------------------
## Adding an AssayLink between two assays
##---------------------------------------

## create a QFeatures object with 2 (identical) assays
## see also '?QFeatures'
se <- SummarizedExperiment(matrix(runif(20), ncol = 2,
                                  dimnames = list(LETTERS[1:10],
                                                  letters[1:2])),
                           rowData = DataFrame(ID = 1:10))
ft <- QFeatures(list(assay1 = se, assay2 = se))

## assay1 and assay2 are not linked
assayLink(ft, "assay2") ## 'from' is NA
assayLink(ft, "assay1") ## 'from' is NA

## Suppose assay2 was generated from assay1 and the feature variable
## 'ID' keeps track of the relationship between the two assays
ftLinked <- addAssayLink(ft, from = "assay1", to = "assay2",
                         varFrom = "ID", varTo = "ID")
assayLink(ftLinked, "assay2")

## For one-to-one relationships, you can also use
ftLinked <- addAssayLinkOneToOne(ft, from = "assay1", to = "assay2")
assayLink(ftLinked, "assay2")

##----------------------------------------
## Adding an AssayLink between more assays
##----------------------------------------

## An assay can also be linked to multiple parent assays
## Create a QFeatures object with 2 parent assays and 1 child assay
ft <- QFeatures(list(parent1 = se[1:6, ], parent2 = se[4:10, ], child = se))
ft <- addAssayLink(ft, from = c("parent1", "parent2"), to = "child",
                   varFrom = c("ID", "ID"), varTo = "ID")
assayLink(ft, "child")

Description

This function counts the number of unique features per sample. A grouping structure can be provided to count higher level features from assays, for example counting the number of unique proteins from PSM data.

Usage

countUniqueFeatures(object, i, groupBy = NULL, colDataName = "count")

Arguments

Value

An object of class QFeatures.

Examples

data("ft_na")
## Count number of (non-missing) PSMs
ft_na <- countUniqueFeatures(ft_na, 
                             i = "na", 
                             colDataName = "counts")
ft_na$counts
## Count number of unique rowData feature
ft_na <- countUniqueFeatures(ft_na, 
                             i = "na", 
                             groupBy = "Y",
                             colDataName = "Y_counts")
ft_na$Y_counts

Description

A shiny app to browser and explore the assays in an MultiAssayExperiment object. Each assay can be selected from the dropdown meny in the side panel, and the quantitative data and row metadata are displayed in the respective Assay and Row data tabs. The Heatmap tab displays a heatmap of the assay. The selection of rows in the Row data table is used to subset the features displayed in the Assay table and the heatmap to those currectly selected. See QFeatures for an example.

Usage

display(object, n = 100, ...)

Arguments

Value

Used for its side effect.

Author(s)

Laurent Gatto

Examples

## Not run: 
data(feat2)
display(feat2)

## End(Not run)

Description

feat1 is a small test QFeatures object for testing and demonstration. feat2 is used to demonstrate assay joins. ft_na is a tiny test set that contains missing values used to demonstrate and test the impact of missing values on data processing. se_na2 is an SummarizedExperiment with missing values of mixed origin.

Usage

feat1

Format

An object of class QFeatures of length 1.

Description

feat3 is a small QFeatures object that contains 7 assays: psms1, psms2, psmsall, peptides, proteins, normpeptides, normproteins. The dataset contains example data that could be obtained after running a simple processing pipeline. You can use it to get your hands on manipulating AssayLinks since all 3 general cases are present:

• One parent to one child AssayLink: the relationship can either be one row to one row (e.g. "peptides" to "normpeptides") or multiple rows to one row (e.g. "peptides" to "proteins").

• One parent to multiple children AssayLink: for instance "peptides" to "normpeptides" and "proteins".

• Multiple parents to one child AssayLink: links the rows between multiple assays to a single assays where some rows in different parent assays may point to the same row in the child assay. E.g. "psms1" and "psms2" to "psmsall"

Usage

feat3

Format

An object of class QFeatures of length 7.

Source

feat3 was built from feat1. The source code is available in inst/scripts/test_data.R

See Also

See ?feat1 for other example/test data sets.

Examples

data("feat3")
plot(feat3)

Description

A data.frame with PSM-level quantitation data by Christoforou et al. (2016). This is the first replicate of a spatial proteomics dataset from a hyperLOPIT experimental design on Mouse E14TG2a embryonic stem cells. Normalised intensities for proteins for TMT 10-plex labelled fractions are available for 3 replicates acquired in MS3 mode using an Orbitrap Fusion mass-spectrometer.

The variable names are

• X126, X127C, X127N, X128C, X128N, X129C, X129N, X130C, X130N and X131: the 10 TMT tags used to quantify the peptides along the density gradient.

• Sequence: the peptide sequence.

• ProteinDescriptions: the description of the protein this peptide was associated to.

• NbProteins: the number of proteins in the protein group.

• ProteinGroupAccessions: the main protein accession number in the protein group.

• Modifications: post-translational modifications identified in the peptide.

• qValue: the PSM identification q-value.

• PEP: the PSM posterior error probability.

• IonScore: the Mascot ion identification score.

• NbMissedCleavages: the number of missed cleavages in the peptide.

• IsolationInterference: the calculated precursor ion isolation interference.

• IonInjectTimems: the ions injection time in milli-seconds.

• Intensity: the precursor ion intensity.

• Charge: the peptide charge.

• mzDa: the peptide mass to charge ratio, in Daltons.

• MHDa: the peptide mass, in Daltons.

• DeltaMassPPM: the difference in measure and calculated mass, in parts per millions.

• RTmin: the peptide retention time, in minutes.

• markers: localisation for well known sub-cellular markers. QFeatures of unknown location are encode as "unknown".

For further details, install the pRolocdata package and see ?hyperLOPIT2015.

Usage

hlpsms

Format

An object of class data.frame with 3010 rows and 28 columns.

Source

The pRolocdata package: http://bioconductor.org/packages/pRolocdata/

References

A draft map of the mouse pluripotent stem cell spatial proteome Christoforou A, Mulvey CM, Breckels LM, Geladaki A, Hurrell T, Hayward PC, Naake T, Gatto L, Viner R, Martinez Arias A, Lilley KS. Nat Commun. 2016 Jan 12;7:8992. doi: 10.1038/ncomms9992. PubMed PMID: 26754106; PubMed Central PMCID: PMC4729960.

See Also

See QFeatures to import this data using the readQFeatures() function.

Description

The impute method performs data imputation on QFeatures and SummarizedExperiment instance using a variety of methods.

Users should proceed with care when imputing data and take precautions to assure that the imputation produce valid results, in particular with naive imputations such as replacing missing values with 0.

See MsCoreUtils::impute_matrix() for details on the different imputation methods available and strategies.

Usage

impute

## S4 method for signature 'SummarizedExperiment'
impute(object, method, ...)

## S4 method for signature 'QFeatures'
impute(object, method, ..., i, name = "imputedAssay")

Arguments

Format

An object of class standardGeneric of length 1.

Examples

MsCoreUtils::imputeMethods()

data(se_na2)
## table of missing values along the rows (proteins)
table(rowData(se_na2)$nNA)
## table of missing values along the columns (samples)
colData(se_na2)$nNA

## non-random missing values
notna <- which(!rowData(se_na2)$randna)
length(notna)
notna

impute(se_na2, method = "min")

if (require("imputeLCMD")) {
  impute(se_na2, method = "QRILC")
  impute(se_na2, method = "MinDet")
}

if (require("norm"))
  impute(se_na2, method = "MLE")

impute(se_na2, method = "mixed",
       randna = rowData(se_na2)$randna,
       mar = "knn", mnar = "QRILC")

## neighbour averaging
x <- se_na2[1:4, 1:6]
assay(x)[1, 1] <- NA ## min value
assay(x)[2, 3] <- NA ## average
assay(x)[3, 1:2] <- NA ## min value and average
## 4th row: no imputation
assay(x)

assay(impute(x, "nbavg"))

Description

This function applies a full-join type of operation on 2 or more assays in a QFeatures instance.

Usage

joinAssays(x, i, name = "joinedAssay")

Arguments

Details

The rows to be joined are chosen based on the rownames of the respective assays. It is the user's responsability to make sure these are meaningful, such as for example refering to unique peptide sequences or proteins.

The join operation acts along the rows and expects the samples (columns) of the assays to be disjoint, i.e. the assays mustn't share any samples. Rows that aren't present in an assay are set to NA when merged.

The rowData slots are also joined. However, only columns that are shared and that have the same values for matching columns/rows are retained. For example of a feature variable A in sample S1 contains value a1 and variable A in sample S2 in a different assay contains a2, then the feature variable A is dropped in the merged assay.

The joined assay is linked to its parent assays through an AssayLink object. The link between the child assay and the parent assays is based on the assay row names, just like the procedure for joining the parent assays.

Value

A QFeatures object with an additional assay.

Author(s)

Laurent Gatto

Examples

## -----------------------------------------------
## An example QFeatures with 3 assays to be joined
## -----------------------------------------------
data(feat2)
feat2

feat2 <- joinAssays(feat2, 1:3)

## Individual assays to be joined, each with 4 samples and a
## variable number of rows.
assay(feat2[[1]])
assay(feat2[[2]])
assay(feat2[[3]])

## The joined assay contains 14 rows (corresponding to the union
## of those in the initial assays) and 12 samples
assay(feat2[["joinedAssay"]])

## The individual rowData to be joined.
rowData(feat2[[1]])
rowData(feat2[[2]])
rowData(feat2[[3]])

## Only the 'Prot' variable is retained because it is shared among
## all assays and the values and coherent across samples (the
## value of 'Prot' for row 'j' is always 'Pj'). The variable 'y' is
## missing in 'assay1' and while variable 'x' is present is all
## assays, the values for the shared rows are different.
rowData(feat2[["joinedAssay"]])

Description

This manual page describes the handling of missing values in QFeatures objects. In the following functions, if object is of class QFeatures, an optional assay index or name i can be specified to define the assay (by name of index) on which to operate.

The following functions are currently available:

• zeroIsNA(object, i) replaces all 0 in object by NA. This is often necessary when third-party software assume that features that weren't quantified should be assigned an intensity of 0.

• infIsNA(object, i) replaces all infinite values in object by NA. This is necessary when third-party software divide expression data by zero values, for instance during custom normalization.

• nNA(object, i) returns a list of missing value summaries. The first element nNA gives a DataFrame with the number and the proportion of missing values for the whole assay; the second element nNArows provides a DataFrame with the number and the proportion of missing values for the features (rows) of the assay(s); the third element nNAcols provides the number and the proportions of missing values in each sample of the assay(s). When object has class QFeatures and additional column with the assays is provided in each element's DataFrame.

• filterNA(object, pNA, i) removes features (rows) that contain a proportion of more missing values of pNA or higher.

See the Processing vignette for examples.

Usage

## S4 method for signature 'SummarizedExperiment,missing'
zeroIsNA(object, i)

## S4 method for signature 'QFeatures,integer'
zeroIsNA(object, i)

## S4 method for signature 'QFeatures,numeric'
zeroIsNA(object, i)

## S4 method for signature 'QFeatures,character'
zeroIsNA(object, i)

## S4 method for signature 'SummarizedExperiment,missing'
infIsNA(object, i)

## S4 method for signature 'QFeatures,integer'
infIsNA(object, i)

## S4 method for signature 'QFeatures,numeric'
infIsNA(object, i)

## S4 method for signature 'QFeatures,character'
infIsNA(object, i)

## S4 method for signature 'SummarizedExperiment,missing'
nNA(object, i)

## S4 method for signature 'QFeatures,integer'
nNA(object, i)

## S4 method for signature 'QFeatures,numeric'
nNA(object, i)

## S4 method for signature 'QFeatures,character'
nNA(object, i)

## S4 method for signature 'SummarizedExperiment'
filterNA(object, pNA = 0)

## S4 method for signature 'QFeatures'
filterNA(object, pNA = 0, i)

Arguments

Value

An instance of the same class as object.

See Also

The impute() for QFeautres instances.

Examples

data(ft_na)

## Summary if missing values
nNA(ft_na, 1)

## Remove rows with missing values
assay(filterNA(ft_na, i = 1))

## Replace NAs by zero and back
ft_na <- impute(ft_na, i = 1, method = "zero")
assay(ft_na)
ft_na <- zeroIsNA(ft_na, 1)
assay(ft_na)

Description

Conceptually, a QFeatures object holds a set of assays, each composed of a matrix (or array) containing quantitative data and row annotations (meta-data). The number and the names of the columns (samples) must always be the same across the assays, but the number and the names of the rows (features) can vary. The assays are typically defined as SummarizedExperiment objects. In addition, a QFeatures object also uses a single DataFrame to annotate the samples (columns) represented in all the matrices.

The QFeatures class extends the MultiAssayExperiment::MultiAssayExperiment and inherits all the functionality of the MultiAssayExperiment::MultiAssayExperiment class.

A typical use case for such QFeatures object is to represent quantitative proteomics (or metabolomics) data, where different assays represent quantitation data at the PSM (the main assay), peptide and protein level, and where peptide values are computed from the PSM data, and the protein-level data is calculated based on the peptide-level values. The largest assay (the one with the highest number of features, PSMs in the example above) is considered the main assay.

The recommended way to create QFeatures objects is the use the readQFeatures() function, that creates an instance from tabular data. The QFeatures constructor can be used to create objects from their bare parts. It is the user's responsability to make sure that these match the class validity requirements.

Usage

QFeatures(..., assayLinks = NULL)

## S4 method for signature 'QFeatures'
show(object)

## S3 method for class 'QFeatures'
plot(x, interactive = FALSE, ...)

## S4 method for signature 'QFeatures,ANY,ANY,ANY'
x[i, j, ..., drop = TRUE]

## S4 method for signature 'QFeatures,character,ANY,ANY'
x[i, j, k, ..., drop = TRUE]

## S4 method for signature 'QFeatures'
c(x, ...)

## S4 method for signature 'QFeatures'
dims(x, use.names = TRUE)

## S4 method for signature 'QFeatures'
nrows(x, use.names = TRUE)

## S4 method for signature 'QFeatures'
ncols(x, use.names = TRUE)

## S4 method for signature 'QFeatures'
rowData(x, use.names = TRUE, ...)

## S4 replacement method for signature 'QFeatures,DataFrameList'
rowData(x) <- value

## S4 replacement method for signature 'QFeatures,ANY'
rowData(x) <- value

rbindRowData(object, i)

selectRowData(x, rowvars)

rowDataNames(x)

## S4 replacement method for signature 'QFeatures,character'
names(x) <- value

longFormat(object, colvars = NULL, rowvars = NULL, index = 1L)

addAssay(x, y, name, assayLinks)

removeAssay(x, i)

replaceAssay(x, y, i)

## S4 replacement method for signature 'QFeatures,ANY,ANY'
x[[i, j, ...]] <- value

## S4 method for signature 'QFeatures'
updateObject(object, ..., verbose = FALSE)

dropEmptyAssays(object, dims = 1:2)

Arguments

Constructors

• QFeatures(..., assayLinks) allows the manual construction of objects. It is the user's responsability to make sure these comply. The arguments in ... are those documented in MultiAssayExperiment::MultiAssayExperiment(). For details about assayLinks, see AssayLinks. An example is shown below.

• The readQFeatures() function constructs a QFeatures object from text-based spreadsheet or a data.frame used to generate an assay. See the function manual page for details and an example.

Accessors

• The QFeatures class extends the MultiAssayExperiment::MultiAssayExperiment class and inherits all its accessors and replacement methods.

• The rowData method returns a DataFrameList containing the rowData for each assay of the QFeatures object. On the other hand, rowData can be modified using rowData(x) <- value, where value is a list of tables that can be coerced to DFrame tables. The names of value point to the assays for which the rowData must be replaced. The column names of each table are used to replace the data in the existing rowData. If the column name does not exist, a new column is added to the rowData.

• The rbindRowData functions returns a DFrame table that contains the row binded rowData tables from the selected assays. In this context, i is a character(), integer() or logical() object for subsetting assays. Only rowData variables that are common to all assays are kept.

• The rowDataNames accessor returns a list with the rowData variable names.

• The longFormat accessor takes a QFeatures object and returns it in a long format DataFrame. Each quantitative value is reported on a separate line. colData and rowData data can also be added. This function is an extension of the longFormat function in the MultiAssayExperiment::MultiAssayExperiment.

Adding, removing and replacing assays

• The aggregateFeatures() function creates a new assay by aggregating features of an existing assay.

• addAssay(x, y, name, assayLinks): Adds one or more new assay(s) y to the QFeatures instance x. name is a character(1) naming the assay if only one assay is provided, and is ignored if y is a list of assays. assayLinks is an optional AssayLinks. The colData(y) is automatically added to colData(x) by matching sample names, that is colnames(y). If the samples are not present in x, the rows of colData(x) are extended to account for the new samples. Be aware that conflicting information between the colData(y) and the colData(x) will result in an error.

• removeAssay(x, i): Removes one or more assay(s) from the QFeatures instance x. In this context, i is a character(), integer() or logical() that indicates which assay(s) to remove.

• replaceAssay(x, y, i): Replaces one or more assay(s) from the QFeatures instance x. In this context, i is a character(), integer() or logical() that indicates which assay(s) to replace. The AssayLinks from or to any replaced assays are automatically removed, unless the replacement has the same dimension names (columns and row, order agnostic). Be aware that conflicting information between colData(y) and colData(x) will result in an error.

• x[[i]] <- value: a generic method for adding (when i is not in names(x)), removing (when value is null) or replacing (when i is in names(x)). Note that the arguments j and ... from the S4 replacement method signature are not allowed.

Subsetting

• QFeatures object can be subset using the x[i, j, k, drop = TRUE] paradigm. In this context, i is a character(), integer(), logical() or GRanges() object for subsetting by rows. See the argument descriptions for details on the remaining arguments.

• The subsetByFeature() function can be used to subset a QFeatures object using one or multiple feature names that will be matched across different assays, taking the aggregation relation between assays.

• The selectRowData(x, rowvars) function can be used to select a limited number of rowData columns of interest named in rowvars in the x instance of class QFeatures. All other variables than rowvars will be dropped. In case an element in rowvars isn't found in any rowData variable, a message is printed.

• The dropEmptyAssays(object, dims) function removes empty assays from a QFeatures. Empty assays are defined as having 0 rows and/or 0 columns, as defined by the dims argument.

Author(s)

Laurent Gatto

See Also

• The readQFeatures() constructor and the aggregateFeatures() function. The QFeatures vignette provides an extended example.

• The QFeatures-filtering manual page demonstrates how to filter features based on their rowData.

• The missing-data manual page to manage missing values in QFeatures objects.

• The QFeatures-processing and aggregateFeatures() manual pages and Processing vignette describe common quantitative data processing methods using in quantitative proteomics.

Examples

## ------------------------
## An empty QFeatures object
## ------------------------

QFeatures()

## -----------------------------------
## Creating a QFeatures object manually
## -----------------------------------

## two assays (matrices) with matching column names
m1 <- matrix(1:40, ncol = 4)
m2 <- matrix(1:16, ncol = 4)
sample_names <- paste0("S", 1:4)
colnames(m1) <- colnames(m2) <- sample_names
rownames(m1) <- letters[1:10]
rownames(m2) <- letters[1:4]

## two corresponding feature metadata with appropriate row names
df1 <- DataFrame(Fa = 1:10, Fb = letters[1:10],
                 row.names = rownames(m1))
df2 <- DataFrame(row.names = rownames(m2))

(se1 <- SummarizedExperiment(m1, df1))
(se2 <- SummarizedExperiment(m2, df2))

## Sample annotation (colData)
cd <- DataFrame(Var1 = rnorm(4),
                Var2 = LETTERS[1:4],
                row.names = sample_names)

el <- list(assay1 = se1, assay2 = se2)
fts1 <- QFeatures(el, colData = cd)
fts1
fts1[[1]]
fts1[["assay1"]]

## Rename assay
names(fts1) <- c("se1", "se2")

## Add an assay
fts1 <- addAssay(fts1, se1[1:2, ], name = "se3")

## Get the assays feature metadata
rowData(fts1)

## Keep only the Fa variable
selectRowData(fts1, rowvars = "Fa")

## -----------------------------------
## See ?readQFeatures to create a
## QFeatures object from a data.frame
## or spreadsheet.
## -----------------------------------

Description

The filterFeatures methods enables users to filter features based on a variable in their rowData. The features matching the filter will be returned as a new object of class QFeatures. The filters can be provided as instances of class AnnotationFilter (see below) or as formulas.

Usage

VariableFilter(field, value, condition = "==", not = FALSE)

## S4 method for signature 'QFeatures,AnnotationFilter'
filterFeatures(object, filter, i, na.rm = FALSE, keep = FALSE, ...)

## S4 method for signature 'QFeatures,formula'
filterFeatures(object, filter, i, na.rm = FALSE, keep = FALSE, ...)

Arguments

Value

An filtered QFeature object.

The filtering procedure

filterFeatures() will go through each assay of the QFeatures object and apply the filtering on the corresponding rowData. Features that do not pass the filter condition are removed from the assay. In some cases, one may want to filter for a variable present in some assay, but not in other. There are two options: either provide keep = FALSE to remove all features for those assays (and thus leaving an empty assay), or provide keep = TRUE to ignore filtering for those assays.

Because features in a QFeatures object are linked between different assays with AssayLinks, the links are automatically updated. However, note that the function doesn't propagate the filter to parent assays. For example, suppose a peptide assay with 4 peptides is linked to a protein assay with 2 proteins (2 peptides mapped per protein) and you apply filterFeatures(). All features pass the filter except for one protein. The peptides mapped to that protein will remain in the QFeatures object. If propagation of the filtering rules to parent assay is desired, you may want to use x[i, , ] instead (see the Subsetting section in ?QFeature).

Variable filters

The variable filters are filters as defined in the AnnotationFilter package. In addition to the pre-defined filter, users can arbitrarily set a field on which to operate. These arbitrary filters operate either on a character variables (as CharacterVariableFilter objects) or numerics (as NumericVariableFilters objects), which can be created with the VariableFilter constructor.

Author(s)

Laurent Gatto

See Also

The QFeatures man page for subsetting and the QFeatures vignette provides an extended example.

Examples

## ----------------------------------------
## Creating character and numberic
## variable filters
## ----------------------------------------

VariableFilter(field = "my_var",
               value = "value_to_keep",
               condition = "==")

VariableFilter(field = "my_num_var",
               value = 0.05,
               condition = "<=")

example(aggregateFeatures)

## ----------------------------------------------------------------
## Filter all features that are associated to the Mitochondrion in
## the location feature variable. This variable is present in all
## assays.
## ----------------------------------------------------------------

## using the forumla interface, exact mathc
filterFeatures(feat1, ~  location == "Mitochondrion")

## using the forumula intefrace, martial match
filterFeatures(feat1, ~startsWith(location, "Mito"))

## using a user-defined character filter
filterFeatures(feat1, VariableFilter("location", "Mitochondrion"))

## using a user-defined character filter with partial match
filterFeatures(feat1, VariableFilter("location", "Mito", "startsWith"))
filterFeatures(feat1, VariableFilter("location", "itochon", "contains"))

## ----------------------------------------------------------------
## Filter all features that aren't marked as unknown (sub-cellular
## location) in the feature variable
## ----------------------------------------------------------------

## using a user-defined character filter
filterFeatures(feat1, VariableFilter("location", "unknown", condition = "!="))

## using the forumula interface
filterFeatures(feat1, ~ location != "unknown")

## ----------------------------------------------------------------
## Filter features that have a p-values lower or equal to 0.03
## ----------------------------------------------------------------

## using a user-defined numeric filter
filterFeatures(feat1, VariableFilter("pval", 0.03, "<="))

## using the formula interface
filterFeatures(feat1, ~ pval <= 0.03)

## you can also remove all p-values that are NA (if any)
filterFeatures(feat1, ~ !is.na(pval))

## ----------------------------------------------------------------
## Negative control - filtering for an non-existing markers value,
## returning empty results.
## ----------------------------------------------------------------

filterFeatures(feat1, VariableFilter("location", "not"))

filterFeatures(feat1, ~ location == "not")

## ----------------------------------------------------------------
## Filtering for a  missing feature variable. The outcome is controled
## by keep
## ----------------------------------------------------------------
data(feat2)

filterFeatures(feat2, ~ y < 0)

filterFeatures(feat2, ~ y < 0, keep = TRUE)

## ----------------------------------------------------------------
## Example with missing values
## ----------------------------------------------------------------

data(feat1)
rowData(feat1[[1]])[1, "location"] <- NA
rowData(feat1[[1]])

## The row with the NA is not removed
rowData(filterFeatures(feat1, ~ location == "Mitochondrion")[[1]])
rowData(filterFeatures(feat1, ~ location == "Mitochondrion", na.rm = FALSE)[[1]])

## The row with the NA is removed
rowData(filterFeatures(feat1, ~ location == "Mitochondrion", na.rm = TRUE)[[1]])

## Note that in situations with missing values, it is possible to
## use the `%in%` operator or filter missing values out
## explicitly.

rowData(filterFeatures(feat1, ~ location %in% "Mitochondrion")[[1]])
rowData(filterFeatures(feat1, ~ location %in% c(NA, "Mitochondrion"))[[1]])

## Explicit handling
filterFeatures(feat1, ~ !is.na(location) & location == "Mitochondrion")

## Using the pipe operator
feat1 |>
   filterFeatures( ~ !is.na(location)) |>
   filterFeatures( ~ location == "Mitochondrion")

Description

This manual page describes common quantitative proteomics data processing methods using QFeatures objects. In the following functions, if object is of class QFeatures, and optional assay index or name i can be specified to define the assay (by name of index) on which to operate.

The following functions are currently available:

• logTransform(object, base = 2, i, pc = 0) log-transforms (with an optional pseudocount offset) the assay(s).

• normalize(object, method, i) normalises the assay(s) according to method (see Details).

• scaleTransform(object, center = TRUE, scale = TRUE, i) applies base::scale() to SummarizedExperiment and QFeatures objects.

• sweep(x, MARGIN, STATS, FUN = "-", check.margin = TRUE, ...) sweeps out array summaries from SummarizedExperiment and QFeatures objects. See base::sweep() for details.

See the Processing vignette for examples.

Usage

## S4 method for signature 'SummarizedExperiment'
logTransform(object, base = 2, pc = 0)

## S4 method for signature 'QFeatures'
logTransform(object, i, name = "logAssay", base = 2, pc = 0)

## S4 method for signature 'SummarizedExperiment'
scaleTransform(object, center = TRUE, scale = TRUE)

## S4 method for signature 'QFeatures'
scaleTransform(object, i, name = "scaledAssay", center = TRUE, scale = TRUE)

## S4 method for signature 'SummarizedExperiment'
normalize(object, method, ...)

## S4 method for signature 'QFeatures'
normalize(object, i, name = "normAssay", method, ...)

## S4 method for signature 'SummarizedExperiment'
sweep(x, MARGIN, STATS, FUN = "-", check.margin = TRUE, ...)

## S4 method for signature 'QFeatures'
sweep(
  x,
  MARGIN,
  STATS,
  FUN = "-",
  check.margin = TRUE,
  ...,
  i,
  name = "sweptAssay"
)

Arguments

Details

The method parameter in normalize can be one of "sum", "max", "center.mean", "center.median", "div.mean", "div.median", "diff.median", ⁠"quantiles⁠", ⁠"quantiles.robust⁠" or "vsn". The MsCoreUtils::normalizeMethods() function returns a vector of available normalisation methods.

• For "sum" and "max", each feature's intensity is divided by the maximum or the sum of the feature respectively. These two methods are applied along the features (rows).

• "center.mean" and "center.median" center the respective sample (column) intensities by subtracting the respective column means or medians. "div.mean" and "div.median" divide by the column means or medians. These are equivalent to sweeping the column means (medians) along MARGIN = 2 with FUN = "-" (for "center.*") or FUN = "/" (for "div.*").

• "diff.median" centers all samples (columns) so that they all match the grand median by subtracting the respective columns medians differences to the grand median.

• Using "quantiles" or "quantiles.robust" applies (robust) quantile normalisation, as implemented in preprocessCore::normalize.quantiles() and preprocessCore::normalize.quantiles.robust(). "vsn" uses the vsn::vsn2() function. Note that the latter also glog-transforms the intensities. See respective manuals for more details and function arguments.

For further details and examples about normalisation, see MsCoreUtils::normalize_matrix().

Value

An processed object of the same class as x or object.

Examples

MsCoreUtils::normalizeMethods()

Description

These functions convert tabular data into dedicated data objets. The readSummarizedExperiment() function takes a file name or data.frame and converts it into a SummarizedExperiment() object. The readQFeatures() function takes a data.frame and converts it into a QFeatures object (see QFeatures() for details). For the latter, two use-cases exist:

• The single-set case will generate a QFeatures object with a single SummarizedExperiment containing all features of the input table.

• The multi-set case will generate a QFeatures object containing multiple SummarizedExperiments, resulting from splitting the input table. This multi-set case is generally used when the input table contains data from multiple runs/batches.

Usage

readSummarizedExperiment(
  assayData,
  quantCols = NULL,
  fnames = NULL,
  ecol = NULL,
  ...
)

readQFeatures(
  assayData,
  colData = NULL,
  quantCols = NULL,
  runCol = NULL,
  name = "quants",
  removeEmptyCols = FALSE,
  verbose = TRUE,
  ecol = NULL,
  ...
)

Arguments

Details

The single- and multi-set cases are defined by the quantCols and runCol parameters, whether passed by the quantCols and runCol vectors and/or the colData data.frame (see below).

Single-set case

The quantitative data variables are defined by the quantCols. The single-set case can be represented schematically as shown below.

|------+----------------+-----------|
| cols | quantCols 1..N | more cols |
| .    | ...            | ...       |
| .    | ...            | ...       |
| .    | ...            | ...       |
|------+----------------+-----------|

Note that every quantCols column contains data for a single sample. The single-set case is defined by the absence of any runCol input (see next section). We here provide a (non-exhaustive) list of typical data sets that fall under the single-set case:

• Peptide- or protein-level label-free data (bulk or single-cell).

• Peptide- or protein-level multiplexed (e.g. TMT) data (bulk or single-cell).

• PSM-level multiplexed data acquired in a single MS run (bulk or single-cell).

• PSM-level data from fractionation experiments, where each fraction of the same sample was acquired with the same multiplexing label.

Multi-set case

A run/batch variable, runCol, is required to import multi-set data. The multi-set case can be represented schematically as shown below.

|--------+------+----------------+-----------|
| runCol | cols | quantCols 1..N | more cols |
|   1    | .    | ...            | ...       |
|   1    | .    | ...            | ...       |
|--------+------+----------------+-----------|
|   2    | .    | ...            | ...       |
|--------+------+----------------+-----------|
|   .    | .    | ...            | ...       |
|--------+------+----------------+-----------|

Every quantCols column contains data for multiple samples acquired in different runs. The multi-set case applies when runCol is provided, which will determine how the table is split into multiple sets.

We here provide a (non-exhaustive) list of typical data sets that fall under the multi-set case:

• PSM- or precursor-level multiplexed data acquired in multiple runs (bulk or single-cell)

• PSM- or precursor-level label-free data acquired in multiple runs (bulk or single-cell)

• DIA-NN data (see also readQFeaturesFromDIANN()).

Adding sample annotations with colData

We recommend providing sample annotations when creating a QFeatures object. The colData is a table in which each row corresponds to a sample and each column provides information about the samples. There is no restriction on the number of columns and on the type of data they should contain. However, we impose one or two columns (depending on the use case) that allow to link the annotations of each sample to its quantitative data:

• Single-set case: the colData must contain a column named quantCols that provides the names of the columns in assayData containing quantitative values for each sample (see single-set cases in the examples).

• Multi-set case: the colData must contain a column named quantCols that provides the names of the columns in assayData with the quantitative values for each sample, and a column named runCol that provides the MS runs/batches in which each sample has been acquired. The entries in colData[["runCol"]] are matched against the entries provided by assayData[[runCol]].

When the quantCols argument is not provided to readQFeatures(), the function will automatically determine the quantCols from colData[["quantCols"]]. Therefore, quantCols and colData cannot be both missing.

Samples that are present in assayData but absent colData will lead to a warning, and the missing entries will be automatically added to the colData and filled with NAs.

When using the quantCols and runCol arguments only (without colData), the colData contains zero columns/variables.

Value

An instance of class QFeatures or SummarizedExperiment::SummarizedExperiment(). For the former, the quantitative sets of each run are stored in SummarizedExperiment::SummarizedExperiment() object.

Author(s)

Laurent Gatto, Christophe Vanderaa

See Also

• The QFeatures (see QFeatures()) class to read about how to manipulate the resulting QFeatures object.

• The readQFeaturesFromDIANN() function to import DIA-NN quantitative data.

Examples

######################################
## Single-set case.

## Load a data.frame with PSM-level data
data(hlpsms)
hlpsms[1:10, c(1, 2, 10:11, 14, 17)]

## Create a QFeatures object with a single psms set
qf1 <- readQFeatures(hlpsms, quantCols = 1:10, name = "psms")
qf1
colData(qf1)

######################################
## Single-set case with colData.

(coldat <- data.frame(var = rnorm(10),
                      quantCols = names(hlpsms)[1:10]))
qf2 <- readQFeatures(hlpsms, colData = coldat)
qf2
colData(qf2)

######################################
## Multi-set case.

## Let's simulate 3 different files/batches for that same input
## data.frame, and define a colData data.frame.

hlpsms$file <- paste0("File", sample(1:3, nrow(hlpsms), replace = TRUE))
hlpsms[1:10, c(1, 2, 10:11, 14, 17, 29)]

qf3 <- readQFeatures(hlpsms, quantCols = 1:10, runCol = "file")
qf3
colData(qf3)


######################################
## Multi-set case with colData.

(coldat <- data.frame(runCol = rep(paste0("File", 1:3), each = 10),
                      var = rnorm(10),
                      quantCols = names(hlpsms)[1:10]))
qf4 <- readQFeatures(hlpsms, colData = coldat, runCol = "file")
qf4
colData(qf4)

Description

This function takes the Report.tsv output files from DIA-NN and converts them into a multi-set QFeatures object. It is a wrapper around readQFeatures() with default parameters set to match DIA-NN label-free and plexDIA report files: default runCol is "File.Name" and default quantColsis"Ms1.Area"'.

Usage

readQFeaturesFromDIANN(
  assayData,
  colData = NULL,
  quantCols = "Ms1.Area",
  runCol = "File.Name",
  multiplexing = c("none", "mTRAQ"),
  extractedData = NULL,
  ecol = NULL,
  verbose = TRUE,
  ...
)

Arguments

Value

An instance of class QFeatures. The quantiative data of each acquisition run is stored in a separate set as a SummarizedExperiment object.

Author(s)

Laurent Gatto, Christophe Vanderaa

References

Derks, Jason, Andrew Leduc, Georg Wallmann, R. Gray Huffman, Matthew Willetts, Saad Khan, Harrison Specht, Markus Ralser, Vadim Demichev, and Nikolai Slavov. 2022. "Increasing the Throughput of Sensitive Proteomics by plexDIA." Nature Biotechnology, July. Link to article

See Also

• The QFeatures (see QFeatures()) class to read about how to manipulate the resulting QFeatures object.

• The readQFeatures() function which this one depends on.

Examples

x <- read.delim(MsDataHub::benchmarkingDIA.tsv())
x[["File.Name"]] <- x[["Run"]]

#################################
## Label-free multi-set case

## using default arguments
readQFeaturesFromDIANN(x)

## with a colData (and default arguments)
cd <- data.frame(sampleInfo = LETTERS[1:24],
                 quantCols = "Ms1.Area",
                 runCol = unique(x[["File.Name"]]))
readQFeaturesFromDIANN(x, colData = cd)

#################################
## mTRAQ multi-set case

x2 <- read.delim(MsDataHub::Report.Derks2022.plexDIA.tsv())
x2[["File.Name"]] <- x2[["Run"]]
readQFeaturesFromDIANN(x2, multiplexing = "mTRAQ")

Description

A long dataframe can be reduced by mergeing certain rows into a single one. These new variables are constructed as a SimpleList containing all the original values. Invariant columns, i.e columns that have the same value along all the rows that need to be merged, can be shrunk into a new variables containing that invariant value (rather than in list columns). The grouping of rows, i.e. the rows that need to be shrunk together as one, is defined by a vector.

The opposite operation is expand. But note that for a DataFrame to be expanded back, it must not to be simplified.

Usage

reduceDataFrame(x, k, count = FALSE, simplify = TRUE, drop = FALSE)

expandDataFrame(x, k = NULL)

Arguments

Value

An expanded (reduced) DataFrame.

Missing values

Missing values do have an important effect on reduce. Unless all values to be reduces are missing, they will result in an non-invariant column, and will be dropped with drop = TRUE. See the example below.

The presence of missing values can have side effects in higher level functions that rely on reduction of DataFrame objects.

Author(s)

Laurent Gatto

Examples

library("IRanges")

k <- sample(100, 1e3, replace = TRUE)
df <- DataFrame(k = k,
                x = round(rnorm(length(k)), 2),
                y = seq_len(length(k)),
                z = sample(LETTERS, length(k), replace = TRUE),
                ir = IRanges(seq_along(k), width = 10),
                r = Rle(sample(5, length(k), replace = TRUE)),
                invar = k + 1)
df

## Shinks the DataFrame
df2 <- reduceDataFrame(df, df$k)
df2

## With a tally of the number of members in each group
reduceDataFrame(df, df$k, count = TRUE)

## Much faster, but more crowded result
df3 <- reduceDataFrame(df, df$k, simplify = FALSE)
df3

## Drop all non-invariant columns
reduceDataFrame(df, df$k, drop = TRUE)

## Missing values
d <- DataFrame(k = rep(1:3, each = 3),
               x = letters[1:9],
               y = rep(letters[1:3], each = 3),
               y2 = rep(letters[1:3], each = 3))
d

## y is invariant and can be simplified
reduceDataFrame(d, d$k)
## y isn't not dropped
reduceDataFrame(d, d$k, drop = TRUE)

## BUT with a missing value
d[1, "y"] <- NA
d

## y isn't invariant/simplified anymore
reduceDataFrame(d, d$k)
## y now gets dropped
reduceDataFrame(d, d$k, drop = TRUE)

Description

This function will find the assays and features that match directly (by name) or indirectly (through aggregation) the feature name.

The subsetByFeature function will first identify the assay that contains the feature(s) i and filter the rows matching these feature names exactly. It will then find, in the other assays, the features that produces i through aggregation with the aggregateQFeatures function.

See QFeatures for an example.

Arguments

Value

An new instance of class QFeatures containing relevant assays and features.

Examples

example(aggregateFeatures)

## Retrieve protein 'ProtA' and its 2 peptides and 6 PSMs
feat1["ProtA", , ]

Description

A data frame is said to be folded when some cells contain multiple elements. These are often encode as a semi-colon separated character , such as "a;b". This function will transform the data frame to that "a" and "b" are split and recorded across two lines.

The simple example below illustrates a trivial case, where the table below

is unfolded based on the Y variable and becomes

where the value 1 of variable X is now duplicated.

If there is a second variable that follows the same pattern as the one used to unfold the table, it also gets unfolded.

becomes

because it is implied that the element in "a;b" are match to "x;y" by their respective indices. Note in the above example, unfolding by Y or Z produces the same result.

However, the following table unfolded by Y

produces

because "c" and "x;y" along the second row don't match. In this case, unfolding by Z would produce a different result. These examples are also illustrated below.

Note that there is no foldDataFrame() function. See reduceDataFrame() and expandDataFrame() to flexibly encode and handle vectors of length > 1 within cells.

Usage

unfoldDataFrame(x, k, split = ";")

Arguments

Value

A new object unfolded object of class class(x) with numbers of rows >= nrow(x) and columns identical to x.

Author(s)

Laurent Gatto

Examples

(x0 <- DataFrame(X = 1:2, Y = c("a;b", "c")))
unfoldDataFrame(x0, "Y")

(x1 <- DataFrame(X = 1:2, Y = c("a;b", "c"), Z = c("x;y", "z")))
unfoldDataFrame(x1, "Y")
unfoldDataFrame(x1, "Z") ## same

(x2 <- DataFrame(X = 1:2, Y = c("a;b", "c"), Z = c("x;y", "x;y")))
unfoldDataFrame(x2, "Y")
unfoldDataFrame(x2, "Z") ## different