Package: visiumStitched Title: Enable downstream analysis of Visium capture areas stitched together with Fiji Version: 1.5.0 Date: 2025-10-22 Authors@R: c( person("Nicholas J.", "Eagles", email = "nickeagles77@gmail.com", role = c("aut", "cre"), comment = c(ORCID = "0000-0002-9808-5254")), person("Leonardo", "Collado-Torres", role = c("ctb"), email = "lcolladotor@gmail.com", comment = c(ORCID = "0000-0003-2140-308X")) ) Description: This package provides helper functions for working with multiple Visium capture areas that overlap each other. This package was developed along with the companion example use case data available from https://github.com/LieberInstitute/visiumStitched_brain. visiumStitched prepares SpaceRanger (10x Genomics) output files so you can stitch the images from groups of capture areas together with Fiji. Then visiumStitched builds a SpatialExperiment object with the stitched data and makes an artificial hexagonal grid enabling the seamless use of spatial clustering methods that rely on such grid to identify neighboring spots, such as PRECAST and BayesSpace. The SpatialExperiment objects created by visiumStitched are compatible with spatialLIBD, which can be used to build interactive websites for stitched SpatialExperiment objects. visiumStitched also enables casting SpatialExperiment objects as Seurat objects. License: Artistic-2.0 Encoding: UTF-8 Roxygen: list(markdown = TRUE) RoxygenNote: 7.3.2 Depends: R (>= 4.4), SpatialExperiment Suggests: BiocFileCache, BiocStyle, ggplot2, knitr, RefManageR, rmarkdown, sessioninfo, Seurat, testthat (>= 3.0.0) Config/testthat/edition: 3 VignetteBuilder: knitr Imports: BiocBaseUtils, BiocGenerics, clue, dplyr, DropletUtils, grDevices, imager, Matrix, methods, pkgcond, readr, rjson, S4Vectors, SingleCellExperiment, spatialLIBD (>= 1.17.8), stringr, SummarizedExperiment, tibble, tidyr, xml2 biocViews: Software, Spatial, Transcriptomics, Transcription, GeneExpression, Visualization, DataImport URL: https://github.com/LieberInstitute/visiumStitched BugReports: https://support.bioconductor.org/tag/visiumStitched Config/pak/sysreqs: cmake libfftw3-dev libglpk-dev make libmagick++-dev gsfonts libbz2-dev libicu-dev libjpeg-dev liblzma-dev libpng-dev libtiff-dev libuv1-dev libxml2-dev libssl-dev perl libx11-dev xz-utils zlib1g-dev Repository: https://bioc.r-universe.dev Date/Publication: 2026-04-28 13:04:03 UTC RemoteUrl: https://github.com/bioc/visiumStitched RemoteRef: HEAD RemoteSha: 4a0e0e4531b5755bd686086f340f3836aeadfc6e NeedsCompilation: no Packaged: 2026-07-03 21:54:45 UTC; root Author: Nicholas J. Eagles [aut, cre] (ORCID: ), Leonardo Collado-Torres [ctb] (ORCID: ) Maintainer: Nicholas J. Eagles