Package: tomoda Title: Tomo-seq data analysis Version: 1.23.0 Authors@R: person(given = "Wendao", family = "Liu", role = c("aut", "cre"), email = "liuwd15@tsinghua.org.cn", comment = c(ORCID = "0000-0002-5124-9338")) Description: This package provides many easy-to-use methods to analyze and visualize tomo-seq data. The tomo-seq technique is based on cryosectioning of tissue and performing RNA-seq on consecutive sections. (Reference: Kruse F, Junker JP, van Oudenaarden A, Bakkers J. Tomo-seq: A method to obtain genome-wide expression data with spatial resolution. Methods Cell Biol. 2016;135:299-307. doi:10.1016/bs.mcb.2016.01.006) The main purpose of the package is to find zones with similar transcriptional profiles and spatially expressed genes in a tomo-seq sample. Several visulization functions are available to create easy-to-modify plots. Depends: R (>= 4.0.0) Imports: methods, stats, grDevices, reshape2, Rtsne, umap, RColorBrewer, ggplot2, ggrepel, SummarizedExperiment License: MIT + file LICENSE Encoding: UTF-8 Roxygen: list(markdown = TRUE) RoxygenNote: 7.1.2 Suggests: knitr, rmarkdown, BiocStyle, testthat URL: https://github.com/liuwd15/tomoda BugReports: https://github.com/liuwd15/tomoda/issues biocViews: GeneExpression, Sequencing, RNASeq, Transcriptomics, Spatial, Clustering, Visualization VignetteBuilder: knitr Config/pak/sysreqs: libicu-dev libpng-dev libssl-dev python3 zlib1g-dev Repository: https://bioc.r-universe.dev Date/Publication: 2026-04-28 12:53:56 UTC RemoteUrl: https://github.com/bioc/tomoda RemoteRef: HEAD RemoteSha: 96ce4ba71e98ef7c1fc2f4122030562b44c1874f NeedsCompilation: no Packaged: 2026-07-04 22:46:37 UTC; root Author: Wendao Liu [aut, cre] (ORCID: ) Maintainer: Wendao Liu