Package: stPipe Type: Package Title: Upstream pre-processing for Sequencing-Based Spatial Transcriptomics Version: 1.3.0 Authors@R: c(person("Yang", "Xu", role=c("aut", "cre"), email="xu.ya@wehi.edu.au", comment=c(ORCID="0009-0008-3274-6516")), person("Callum", "Sargeant", role=c("aut"), email="sargeant.ca@wehi.edu.au"), person("Shian", "Su", role=c("aut"), email="su.s@wehi.edu.au"), person("Luyi", "Tian", role=c("aut"), email="tian.l@wehi.edu.au"), person("Yunshun", "Chen", role=c("ctb"), email="yuchen@wehi.edu.au"), person("Matthew", "Ritchie", role=c("ctb", "fnd"), email="mritchie@wehi.edu.au")) biocViews: ImmunoOncology, Software, Sequencing, RNASeq, GeneExpression, SingleCell, Visualization, SequenceMatching, Preprocessing, QualityControl, GenomeAnnotation, DataImport, Spatial, Transcriptomics, Clustering Description: This package serves as an upstream pipeline for pre-processing sequencing-based spatial transcriptomics data. Functions includes FASTQ trimming, BAM file reformatting, index building, spatial barcode detection, demultiplexing, gene count matrix generation with UMI deduplication, QC, and revelant visualization. Config is an essential input for most of the functions which aims to improve reproducibility. Depends: R (>= 4.5.0) Imports: basilisk, data.table, DropletUtils, dplyr, ggplot2, methods, pbmcapply, reticulate, rmarkdown, Rcpp, Rhtslib, Rsubread, Rtsne, Seurat, SeuratObject, scPipe, shiny, SummarizedExperiment, SingleCellExperiment, SpatialExperiment, stats, umap, yaml LinkingTo: Rcpp, Rhdf5lib, testthat, Rhtslib SystemRequirements: GNU make License: GPL-3 Encoding: UTF-8 RoxygenNote: 7.3.2 URL: https://github.com/mritchielab/stPipe BugReports: https://github.com/mritchielab/stPipe/issues/new Suggests: knitr, plotly, BiocStyle, testthat (>= 3.0.0) VignetteBuilder: knitr Config/testthat/edition: 3 Config/pak/sysreqs: cmake libglpk-dev make libmagick++-dev gsfonts libbz2-dev libicu-dev liblzma-dev libpng-dev libuv1-dev libxml2-dev libssl-dev python3 xz-utils zlib1g-dev Repository: https://bioc.r-universe.dev Date/Publication: 2026-04-28 13:05:24 UTC RemoteUrl: https://github.com/bioc/stPipe RemoteRef: HEAD RemoteSha: 287ceed10e841dece4bd6886acc006fb43096d94 NeedsCompilation: yes Packaged: 2026-07-04 14:44:59 UTC; root Author: Yang Xu [aut, cre] (ORCID: ), Callum Sargeant [aut], Shian Su [aut], Luyi Tian [aut], Yunshun Chen [ctb], Matthew Ritchie [ctb, fnd] Maintainer: Yang Xu