Package: rnaseqGene Title: RNA-seq workflow: gene-level exploratory analysis and differential expression Version: 1.37.0 Date: 2026-04-13 Authors@R: c(person(role=c("aut", "cre"), "Michael", "Love", email = "michaelisaiahlove@gmail.com")) Description: Here we walk through an end-to-end gene-level RNA-seq differential expression workflow using Bioconductor packages. We will start from the FASTQ files, show how these were aligned to the reference genome, and prepare a count matrix which tallies the number of RNA-seq reads/fragments within each gene for each sample. We will perform exploratory data analysis (EDA) for quality assessment and to explore the relationship between samples, perform differential gene expression analysis, and visually explore the results. Depends: R (>= 3.3.0), BiocStyle, airway (>= 1.5.3), tximeta, magrittr, DESeq2, apeglm, vsn, dplyr, ggplot2, hexbin, pheatmap, RColorBrewer, PoiClaClu, glmpca, ggbeeswarm, genefilter, AnnotationDbi, org.Hs.eg.db, Gviz, sva, RUVSeq, fission License: Artistic-2.0 Encoding: UTF-8 VignetteBuilder: knitr biocViews: Workflow, GeneExpressionWorkflow, ImmunoOncologyWorkflow Suggests: knitr, rmarkdown NeedsCompilation: no URL: https://github.com/thelovelab/rnaseqGene/ Config/pak/sysreqs: cmake make libbz2-dev libicu-dev libjpeg-dev liblzma-dev libpng-dev libuv1-dev libxml2-dev libssl-dev xz-utils zlib1g-dev Repository: https://bioc.r-universe.dev Date/Publication: 2026-04-28 12:43:10 UTC RemoteUrl: https://github.com/bioc/rnaseqGene RemoteRef: HEAD RemoteSha: adf76f21025db6b414766adcdd1990f19e4d770f Packaged: 2026-10-01 15:40:13 UTC; root Author: Michael Love [aut, cre] Maintainer: Michael Love