{"Primer":"Tiller_5'LVH1","Template":"IGHV1-18","Group":"IGHV1-18","Number_of_mismatches":1,"primer_efficiency":0.30797,"annealing_DeltaG":-16.688,"Position_3terminus":4,"All_mismatches":"4","Worst_Case_MismatchLocal":"3","Primer_Sequence":"acaggtgcccactcccaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.66667,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":68.06,"Self_Dimer_DeltaG":-0.765,"Structure_deltaG_fw":-1.18,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":3,"Number_of_mismatches_hexamer":1,"_row":"1_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH1","Template":"IGHV1-2","Group":"IGHV1-2","Number_of_mismatches":1,"primer_efficiency":0.99993,"annealing_DeltaG":-16.786,"Position_3terminus":19,"All_mismatches":"19","Worst_Case_MismatchLocal":"0","Primer_Sequence":"acaggtgcccactcccaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.66667,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":68.06,"Self_Dimer_DeltaG":-0.765,"Structure_deltaG_fw":-1.18,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"2_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH1","Template":"IGHV1-24","Group":"IGHV1-24","Number_of_mismatches":4,"primer_efficiency":0.18337,"annealing_DeltaG":-10.796,"Position_3terminus":4,"All_mismatches":"19,18,12,4","Worst_Case_MismatchLocal":"3,3,3,3","Primer_Sequence":"acaggtgcccactcccaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.66667,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":68.06,"Self_Dimer_DeltaG":-0.765,"Structure_deltaG_fw":-1.18,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":3,"Number_of_mismatches_hexamer":1,"_row":"3_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH1","Template":"IGHV1-3","Group":"IGHV1-3","Number_of_mismatches":1,"primer_efficiency":0.30799,"annealing_DeltaG":-17.238,"Position_3terminus":4,"All_mismatches":"4","Worst_Case_MismatchLocal":"3","Primer_Sequence":"acaggtgcccactcccaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.66667,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":68.06,"Self_Dimer_DeltaG":-0.765,"Structure_deltaG_fw":-1.18,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":3,"Number_of_mismatches_hexamer":1,"_row":"4_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH1","Template":"IGHV1-45","Group":"IGHV1-45","Number_of_mismatches":3,"primer_efficiency":0.6066,"annealing_DeltaG":-10.505,"Position_3terminus":6,"All_mismatches":"20,15,6","Worst_Case_MismatchLocal":"1,1,1","Primer_Sequence":"acaggtgcccactcccaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.66667,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":68.06,"Self_Dimer_DeltaG":-0.765,"Structure_deltaG_fw":-1.18,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":1,"Number_of_mismatches_hexamer":1,"_row":"5_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH1","Template":"IGHV1-46","Group":"IGHV1-46","Number_of_mismatches":2,"primer_efficiency":0.99935,"annealing_DeltaG":-15.127,"Position_3terminus":16,"All_mismatches":"24,16","Worst_Case_MismatchLocal":"0,0","Primer_Sequence":"acaggtgcccactcccaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.66667,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":68.06,"Self_Dimer_DeltaG":-0.765,"Structure_deltaG_fw":-1.18,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"6_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH1","Template":"IGHV1-58","Group":"IGHV1-58","Number_of_mismatches":2,"primer_efficiency":0.99879,"annealing_DeltaG":-14.955,"Position_3terminus":6,"All_mismatches":"7,6","Worst_Case_MismatchLocal":"1,1","Primer_Sequence":"acaggtgcccactcccaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.66667,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":68.06,"Self_Dimer_DeltaG":-0.765,"Structure_deltaG_fw":-1.18,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":1,"Number_of_mismatches_hexamer":1,"_row":"7_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH1","Template":"IGHV1-69","Group":"IGHV1-69","Number_of_mismatches":2,"primer_efficiency":0.64783,"annealing_DeltaG":-10.632,"Position_3terminus":13,"All_mismatches":"17,13","Worst_Case_MismatchLocal":"0,0","Primer_Sequence":"acaggtgcccactcccaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.66667,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":68.06,"Self_Dimer_DeltaG":-0.765,"Structure_deltaG_fw":-1.18,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"8_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH1","Template":"IGHV1-69-2","Group":"IGHV1-69-2","Number_of_mismatches":5,"primer_efficiency":0.00116,"annealing_DeltaG":-6.792,"Position_3terminus":4,"All_mismatches":"19,18,12,9,4","Worst_Case_MismatchLocal":"3,3,3,3,3","Primer_Sequence":"acaggtgcccactcccaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.66667,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":68.06,"Self_Dimer_DeltaG":-0.765,"Structure_deltaG_fw":-1.18,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":3,"Number_of_mismatches_hexamer":1,"_row":"9_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH1","Template":"IGHV1-8","Group":"IGHV1-8","Number_of_mismatches":1,"primer_efficiency":0.99994,"annealing_DeltaG":-16.755,"Position_3terminus":21,"All_mismatches":"21","Worst_Case_MismatchLocal":"0","Primer_Sequence":"acaggtgcccactcccaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.66667,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":68.06,"Self_Dimer_DeltaG":-0.765,"Structure_deltaG_fw":-1.18,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"10_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH1","Template":"IGHV2-26","Group":"IGHV2-26","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-2.52,"Position_3terminus":2,"All_mismatches":"21,20,18,17,15,12,10,8,7,6,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"acaggtgcccactcccaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.66667,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":68.06,"Self_Dimer_DeltaG":-0.765,"Structure_deltaG_fw":-1.18,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":2,"_row":"11_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH1","Template":"IGHV2-5","Group":"IGHV2-5","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-1.388,"Position_3terminus":1,"All_mismatches":"20,19,18,16,12,10,9,7,6,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"acaggtgcccactcccaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.66667,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":68.06,"Self_Dimer_DeltaG":-0.765,"Structure_deltaG_fw":-1.18,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":2,"_row":"12_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH1","Template":"IGHV2-70","Group":"IGHV2-70","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-1.388,"Position_3terminus":1,"All_mismatches":"20,19,18,16,12,10,9,7,6,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"acaggtgcccactcccaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.66667,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":68.06,"Self_Dimer_DeltaG":-0.765,"Structure_deltaG_fw":-1.18,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":2,"_row":"13_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH1","Template":"IGHV3-11","Group":"IGHV3-11","Number_of_mismatches":5,"primer_efficiency":0.00169,"annealing_DeltaG":-6.047,"Position_3terminus":10,"All_mismatches":"23,17,13,11,10","Worst_Case_MismatchLocal":"0,0,0,0,0","Primer_Sequence":"acaggtgcccactcccaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.66667,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":68.06,"Self_Dimer_DeltaG":-0.765,"Structure_deltaG_fw":-1.18,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"14_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH1","Template":"IGHV3-13","Group":"IGHV3-13","Number_of_mismatches":7,"primer_efficiency":0.00056,"annealing_DeltaG":-5.208,"Position_3terminus":9,"All_mismatches":"24,23,17,13,11,10,9","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0","Primer_Sequence":"acaggtgcccactcccaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.66667,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":68.06,"Self_Dimer_DeltaG":-0.765,"Structure_deltaG_fw":-1.18,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"15_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH1","Template":"IGHV3-15","Group":"IGHV3-15","Number_of_mismatches":6,"primer_efficiency":0.00056,"annealing_DeltaG":-5.208,"Position_3terminus":9,"All_mismatches":"23,17,13,11,10,9","Worst_Case_MismatchLocal":"0,0,0,0,0,0","Primer_Sequence":"acaggtgcccactcccaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.66667,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":68.06,"Self_Dimer_DeltaG":-0.765,"Structure_deltaG_fw":-1.18,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"16_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH1","Template":"IGHV3-20","Group":"IGHV3-20","Number_of_mismatches":6,"primer_efficiency":0.00056,"annealing_DeltaG":-5.208,"Position_3terminus":9,"All_mismatches":"23,17,13,11,10,9","Worst_Case_MismatchLocal":"0,0,0,0,0,0","Primer_Sequence":"acaggtgcccactcccaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.66667,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":68.06,"Self_Dimer_DeltaG":-0.765,"Structure_deltaG_fw":-1.18,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"17_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH1","Template":"IGHV3-21","Group":"IGHV3-21","Number_of_mismatches":7,"primer_efficiency":0.00056,"annealing_DeltaG":-5.208,"Position_3terminus":9,"All_mismatches":"24,23,17,13,11,10,9","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0","Primer_Sequence":"acaggtgcccactcccaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.66667,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":68.06,"Self_Dimer_DeltaG":-0.765,"Structure_deltaG_fw":-1.18,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"18_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH1","Template":"IGHV3-23","Group":"IGHV3-23","Number_of_mismatches":6,"primer_efficiency":0.00056,"annealing_DeltaG":-5.208,"Position_3terminus":9,"All_mismatches":"23,17,13,11,10,9","Worst_Case_MismatchLocal":"0,0,0,0,0,0","Primer_Sequence":"acaggtgcccactcccaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.66667,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":68.06,"Self_Dimer_DeltaG":-0.765,"Structure_deltaG_fw":-1.18,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"19_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH1","Template":"IGHV3-30","Group":"IGHV3-30","Number_of_mismatches":5,"primer_efficiency":0.00169,"annealing_DeltaG":-6.047,"Position_3terminus":10,"All_mismatches":"23,17,13,11,10","Worst_Case_MismatchLocal":"0,0,0,0,0","Primer_Sequence":"acaggtgcccactcccaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.66667,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":68.06,"Self_Dimer_DeltaG":-0.765,"Structure_deltaG_fw":-1.18,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"20_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH1","Template":"IGHV3-30-3","Group":"IGHV3-30-3","Number_of_mismatches":5,"primer_efficiency":0.00169,"annealing_DeltaG":-6.047,"Position_3terminus":10,"All_mismatches":"23,17,13,11,10","Worst_Case_MismatchLocal":"0,0,0,0,0","Primer_Sequence":"acaggtgcccactcccaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.66667,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":68.06,"Self_Dimer_DeltaG":-0.765,"Structure_deltaG_fw":-1.18,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"21_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH1","Template":"IGHV3-33","Group":"IGHV3-33","Number_of_mismatches":5,"primer_efficiency":0.00169,"annealing_DeltaG":-6.047,"Position_3terminus":10,"All_mismatches":"23,17,13,11,10","Worst_Case_MismatchLocal":"0,0,0,0,0","Primer_Sequence":"acaggtgcccactcccaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.66667,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":68.06,"Self_Dimer_DeltaG":-0.765,"Structure_deltaG_fw":-1.18,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"22_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH1","Template":"IGHV3-43","Group":"IGHV3-43","Number_of_mismatches":7,"primer_efficiency":0.00002,"annealing_DeltaG":-2.877,"Position_3terminus":7,"All_mismatches":"23,17,13,11,10,9,7","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0","Primer_Sequence":"acaggtgcccactcccaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Unamplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.66667,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":68.06,"Self_Dimer_DeltaG":-0.765,"Structure_deltaG_fw":-1.18,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"23_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH1","Template":"IGHV3-48","Group":"IGHV3-48","Number_of_mismatches":7,"primer_efficiency":0.00056,"annealing_DeltaG":-5.208,"Position_3terminus":9,"All_mismatches":"24,23,17,13,11,10,9","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0","Primer_Sequence":"acaggtgcccactcccaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.66667,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":68.06,"Self_Dimer_DeltaG":-0.765,"Structure_deltaG_fw":-1.18,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"24_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH1","Template":"IGHV3-49","Group":"IGHV3-49","Number_of_mismatches":6,"primer_efficiency":0.00056,"annealing_DeltaG":-5.208,"Position_3terminus":9,"All_mismatches":"23,17,13,11,10,9","Worst_Case_MismatchLocal":"0,0,0,0,0,0","Primer_Sequence":"acaggtgcccactcccaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.66667,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":68.06,"Self_Dimer_DeltaG":-0.765,"Structure_deltaG_fw":-1.18,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"25_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH1","Template":"IGHV3-53","Group":"IGHV3-53","Number_of_mismatches":6,"primer_efficiency":0.00056,"annealing_DeltaG":-5.208,"Position_3terminus":9,"All_mismatches":"23,17,13,11,10,9","Worst_Case_MismatchLocal":"0,0,0,0,0,0","Primer_Sequence":"acaggtgcccactcccaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.66667,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":68.06,"Self_Dimer_DeltaG":-0.765,"Structure_deltaG_fw":-1.18,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"26_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH1","Template":"IGHV3-64","Group":"IGHV3-64","Number_of_mismatches":6,"primer_efficiency":0.00056,"annealing_DeltaG":-5.208,"Position_3terminus":9,"All_mismatches":"23,17,13,11,10,9","Worst_Case_MismatchLocal":"0,0,0,0,0,0","Primer_Sequence":"acaggtgcccactcccaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.66667,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":68.06,"Self_Dimer_DeltaG":-0.765,"Structure_deltaG_fw":-1.18,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"27_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH1","Template":"IGHV3-64D","Group":"IGHV3-64D","Number_of_mismatches":7,"primer_efficiency":0.00056,"annealing_DeltaG":-5.208,"Position_3terminus":9,"All_mismatches":"23,20,17,13,11,10,9","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0","Primer_Sequence":"acaggtgcccactcccaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.66667,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":68.06,"Self_Dimer_DeltaG":-0.765,"Structure_deltaG_fw":-1.18,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"28_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH1","Template":"IGHV3-66","Group":"IGHV3-66","Number_of_mismatches":6,"primer_efficiency":0.00056,"annealing_DeltaG":-5.208,"Position_3terminus":9,"All_mismatches":"23,17,13,11,10,9","Worst_Case_MismatchLocal":"0,0,0,0,0,0","Primer_Sequence":"acaggtgcccactcccaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.66667,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":68.06,"Self_Dimer_DeltaG":-0.765,"Structure_deltaG_fw":-1.18,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"29_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH1","Template":"IGHV3-7","Group":"IGHV3-7","Number_of_mismatches":7,"primer_efficiency":0.00056,"annealing_DeltaG":-5.208,"Position_3terminus":9,"All_mismatches":"24,23,17,13,11,10,9","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0","Primer_Sequence":"acaggtgcccactcccaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.66667,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":68.06,"Self_Dimer_DeltaG":-0.765,"Structure_deltaG_fw":-1.18,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"30_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH1","Template":"IGHV3-72","Group":"IGHV3-72","Number_of_mismatches":7,"primer_efficiency":0.00056,"annealing_DeltaG":-5.208,"Position_3terminus":9,"All_mismatches":"24,23,17,13,11,10,9","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0","Primer_Sequence":"acaggtgcccactcccaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.66667,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":68.06,"Self_Dimer_DeltaG":-0.765,"Structure_deltaG_fw":-1.18,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"31_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH1","Template":"IGHV3-73","Group":"IGHV3-73","Number_of_mismatches":6,"primer_efficiency":0.00056,"annealing_DeltaG":-5.208,"Position_3terminus":9,"All_mismatches":"23,17,13,11,10,9","Worst_Case_MismatchLocal":"0,0,0,0,0,0","Primer_Sequence":"acaggtgcccactcccaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.66667,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":68.06,"Self_Dimer_DeltaG":-0.765,"Structure_deltaG_fw":-1.18,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"32_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH1","Template":"IGHV3-74","Group":"IGHV3-74","Number_of_mismatches":6,"primer_efficiency":0.00056,"annealing_DeltaG":-5.208,"Position_3terminus":9,"All_mismatches":"23,17,13,11,10,9","Worst_Case_MismatchLocal":"0,0,0,0,0,0","Primer_Sequence":"acaggtgcccactcccaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.66667,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":68.06,"Self_Dimer_DeltaG":-0.765,"Structure_deltaG_fw":-1.18,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"33_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH1","Template":"IGHV3-9","Group":"IGHV3-9","Number_of_mismatches":7,"primer_efficiency":0.00002,"annealing_DeltaG":-2.877,"Position_3terminus":7,"All_mismatches":"23,17,13,11,10,9,7","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0","Primer_Sequence":"acaggtgcccactcccaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.66667,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":68.06,"Self_Dimer_DeltaG":-0.765,"Structure_deltaG_fw":-1.18,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"34_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH1","Template":"IGHV4-28","Group":"IGHV4-28","Number_of_mismatches":7,"primer_efficiency":0.00039,"annealing_DeltaG":-5.735,"Position_3terminus":4,"All_mismatches":"23,21,19,17,14,13,4","Worst_Case_MismatchLocal":"3,3,3,3,3,3,3","Primer_Sequence":"acaggtgcccactcccaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Unamplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.66667,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":68.06,"Self_Dimer_DeltaG":-0.765,"Structure_deltaG_fw":-1.18,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":3,"Number_of_mismatches_hexamer":1,"_row":"35_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH1","Template":"IGHV4-30-2","Group":"IGHV4-30-2","Number_of_mismatches":7,"primer_efficiency":0.00286,"annealing_DeltaG":-6.863,"Position_3terminus":6,"All_mismatches":"23,21,19,17,14,13,6","Worst_Case_MismatchLocal":"1,1,1,1,1,1,1","Primer_Sequence":"acaggtgcccactcccaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.66667,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":68.06,"Self_Dimer_DeltaG":-0.765,"Structure_deltaG_fw":-1.18,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":1,"Number_of_mismatches_hexamer":1,"_row":"36_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH1","Template":"IGHV4-31","Group":"IGHV4-31","Number_of_mismatches":6,"primer_efficiency":0.00314,"annealing_DeltaG":-8.603,"Position_3terminus":13,"All_mismatches":"23,21,19,17,14,13","Worst_Case_MismatchLocal":"0,0,0,0,0,0","Primer_Sequence":"acaggtgcccactcccaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.66667,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":68.06,"Self_Dimer_DeltaG":-0.765,"Structure_deltaG_fw":-1.18,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"37_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH1","Template":"IGHV4-34","Group":"IGHV4-34","Number_of_mismatches":6,"primer_efficiency":0.00314,"annealing_DeltaG":-8.603,"Position_3terminus":13,"All_mismatches":"23,21,19,17,14,13","Worst_Case_MismatchLocal":"0,0,0,0,0,0","Primer_Sequence":"acaggtgcccactcccaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.66667,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":68.06,"Self_Dimer_DeltaG":-0.765,"Structure_deltaG_fw":-1.18,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"38_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH1","Template":"IGHV4-38-2","Group":"IGHV4-38-2","Number_of_mismatches":6,"primer_efficiency":0.00314,"annealing_DeltaG":-8.603,"Position_3terminus":13,"All_mismatches":"23,21,19,17,14,13","Worst_Case_MismatchLocal":"0,0,0,0,0,0","Primer_Sequence":"acaggtgcccactcccaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.66667,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":68.06,"Self_Dimer_DeltaG":-0.765,"Structure_deltaG_fw":-1.18,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"39_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH1","Template":"IGHV4-39","Group":"IGHV4-39","Number_of_mismatches":7,"primer_efficiency":0.00286,"annealing_DeltaG":-6.863,"Position_3terminus":6,"All_mismatches":"23,21,19,17,14,13,6","Worst_Case_MismatchLocal":"1,1,1,1,1,1,1","Primer_Sequence":"acaggtgcccactcccaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.66667,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":68.06,"Self_Dimer_DeltaG":-0.765,"Structure_deltaG_fw":-1.18,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":1,"Number_of_mismatches_hexamer":1,"_row":"40_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH1","Template":"IGHV4-4","Group":"IGHV4-4","Number_of_mismatches":6,"primer_efficiency":0.00314,"annealing_DeltaG":-8.603,"Position_3terminus":13,"All_mismatches":"23,21,19,17,14,13","Worst_Case_MismatchLocal":"0,0,0,0,0,0","Primer_Sequence":"acaggtgcccactcccaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.66667,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":68.06,"Self_Dimer_DeltaG":-0.765,"Structure_deltaG_fw":-1.18,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"41_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH1","Template":"IGHV4-59","Group":"IGHV4-59","Number_of_mismatches":6,"primer_efficiency":0.00314,"annealing_DeltaG":-8.603,"Position_3terminus":13,"All_mismatches":"23,21,19,17,14,13","Worst_Case_MismatchLocal":"0,0,0,0,0,0","Primer_Sequence":"acaggtgcccactcccaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.66667,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":68.06,"Self_Dimer_DeltaG":-0.765,"Structure_deltaG_fw":-1.18,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"42_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH1","Template":"IGHV4-61","Group":"IGHV4-61","Number_of_mismatches":6,"primer_efficiency":0.00314,"annealing_DeltaG":-8.603,"Position_3terminus":13,"All_mismatches":"23,21,19,17,14,13","Worst_Case_MismatchLocal":"0,0,0,0,0,0","Primer_Sequence":"acaggtgcccactcccaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.66667,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":68.06,"Self_Dimer_DeltaG":-0.765,"Structure_deltaG_fw":-1.18,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"43_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH1","Template":"IGHV5-10-1","Group":"IGHV5-10-1","Number_of_mismatches":10,"primer_efficiency":0.00002,"annealing_DeltaG":-2.956,"Position_3terminus":7,"All_mismatches":"24,23,19,17,15,14,13,12,9,7","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0,0,0,0","Primer_Sequence":"acaggtgcccactcccaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Unamplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.66667,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":68.06,"Self_Dimer_DeltaG":-0.765,"Structure_deltaG_fw":-1.18,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"44_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH1","Template":"IGHV5-51","Group":"IGHV5-51","Number_of_mismatches":9,"primer_efficiency":0.00056,"annealing_DeltaG":-5.208,"Position_3terminus":9,"All_mismatches":"24,23,19,17,15,14,13,12,9","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0,0,0","Primer_Sequence":"acaggtgcccactcccaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.66667,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":68.06,"Self_Dimer_DeltaG":-0.765,"Structure_deltaG_fw":-1.18,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"45_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH1","Template":"IGHV6-1","Group":"IGHV6-1","Number_of_mismatches":8,"primer_efficiency":7.99191e-06,"annealing_DeltaG":-3.192,"Position_3terminus":4,"All_mismatches":"24,23,22,17,14,13,10,4","Worst_Case_MismatchLocal":"3,3,3,3,3,3,3,3","Primer_Sequence":"acaggtgcccactcccaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Unamplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.66667,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":68.06,"Self_Dimer_DeltaG":-0.765,"Structure_deltaG_fw":-1.18,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":3,"Number_of_mismatches_hexamer":1,"_row":"46_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH1","Template":"IGHV7-4-1","Group":"IGHV7-4-1","Number_of_mismatches":0,"primer_efficiency":1,"annealing_DeltaG":-19.589,"All_mismatches":"NA","Worst_Case_MismatchLocal":"0","Primer_Sequence":"acaggtgcccactcccaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.66667,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":68.06,"Self_Dimer_DeltaG":-0.765,"Structure_deltaG_fw":-1.18,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"47_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH3","Template":"IGHV1-18","Group":"IGHV1-18","Number_of_mismatches":7,"primer_efficiency":0.00005,"annealing_DeltaG":-4.446,"Position_3terminus":4,"All_mismatches":"23,17,13,11,10,9,4","Worst_Case_MismatchLocal":"3,3,3,3,3,3,3","Primer_Sequence":"aaggtgtccagtgtgargtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Amplified","primer_length_fw":23,"gc_clamp_fw":1,"gc_ratio_fw":0.54348,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":60.96,"Self_Dimer_DeltaG":-1.22,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":3,"Number_of_mismatches_hexamer":1,"_row":"48_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH3","Template":"IGHV1-2","Group":"IGHV1-2","Number_of_mismatches":7,"primer_efficiency":0.0006,"annealing_DeltaG":-5.255,"Position_3terminus":9,"All_mismatches":"23,19,17,13,11,10,9","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0","Primer_Sequence":"aaggtgtccagtgtgargtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":23,"gc_clamp_fw":1,"gc_ratio_fw":0.54348,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":60.96,"Self_Dimer_DeltaG":-1.22,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"49_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH3","Template":"IGHV1-24","Group":"IGHV1-24","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-2.904,"Position_3terminus":4,"All_mismatches":"23,19,18,17,13,12,11,10,9,4","Worst_Case_MismatchLocal":"3,3,3,3,3,3,3,3,3,3","Primer_Sequence":"aaggtgtccagtgtgargtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Unamplified","primer_length_fw":23,"gc_clamp_fw":1,"gc_ratio_fw":0.54348,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":60.96,"Self_Dimer_DeltaG":-1.22,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":3,"Number_of_mismatches_hexamer":1,"_row":"50_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH3","Template":"IGHV1-3","Group":"IGHV1-3","Number_of_mismatches":7,"primer_efficiency":0.00005,"annealing_DeltaG":-4.446,"Position_3terminus":4,"All_mismatches":"23,17,13,11,10,9,4","Worst_Case_MismatchLocal":"3,3,3,3,3,3,3","Primer_Sequence":"aaggtgtccagtgtgargtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Amplified","primer_length_fw":23,"gc_clamp_fw":1,"gc_ratio_fw":0.54348,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":60.96,"Self_Dimer_DeltaG":-1.22,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":3,"Number_of_mismatches_hexamer":1,"_row":"51_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH3","Template":"IGHV1-45","Group":"IGHV1-45","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-2.713,"Position_3terminus":6,"All_mismatches":"23,20,17,15,13,11,10,9,6","Worst_Case_MismatchLocal":"1,1,1,1,1,1,1,1,1","Primer_Sequence":"aaggtgtccagtgtgargtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Amplified","primer_length_fw":23,"gc_clamp_fw":1,"gc_ratio_fw":0.54348,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":60.96,"Self_Dimer_DeltaG":-1.22,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":1,"Number_of_mismatches_hexamer":1,"_row":"52_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH3","Template":"IGHV1-46","Group":"IGHV1-46","Number_of_mismatches":7,"primer_efficiency":0.0006,"annealing_DeltaG":-5.255,"Position_3terminus":9,"All_mismatches":"23,17,16,13,11,10,9","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0","Primer_Sequence":"aaggtgtccagtgtgargtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":23,"gc_clamp_fw":1,"gc_ratio_fw":0.54348,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":60.96,"Self_Dimer_DeltaG":-1.22,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"53_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH3","Template":"IGHV1-58","Group":"IGHV1-58","Number_of_mismatches":7,"primer_efficiency":0.00016,"annealing_DeltaG":-4.446,"Position_3terminus":6,"All_mismatches":"23,17,13,11,10,9,6","Worst_Case_MismatchLocal":"1,1,1,1,1,1,1","Primer_Sequence":"aaggtgtccagtgtgargtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Amplified","primer_length_fw":23,"gc_clamp_fw":1,"gc_ratio_fw":0.54348,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":60.96,"Self_Dimer_DeltaG":-1.22,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":1,"Number_of_mismatches_hexamer":1,"_row":"54_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH3","Template":"IGHV1-69","Group":"IGHV1-69","Number_of_mismatches":4,"primer_efficiency":0.10116,"annealing_DeltaG":-8.791,"Position_3terminus":9,"All_mismatches":"23,11,10,9","Worst_Case_MismatchLocal":"0,0,0,0","Primer_Sequence":"aaggtgtccagtgtgargtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":23,"gc_clamp_fw":1,"gc_ratio_fw":0.54348,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":60.96,"Self_Dimer_DeltaG":-1.22,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"55_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH3","Template":"IGHV1-69-2","Group":"IGHV1-69-2","Number_of_mismatches":9,"primer_efficiency":5.6114e-06,"annealing_DeltaG":-2.96,"Position_3terminus":4,"All_mismatches":"23,19,18,17,13,12,11,10,4","Worst_Case_MismatchLocal":"3,3,3,3,3,3,3,3,3","Primer_Sequence":"aaggtgtccagtgtgargtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Unamplified","primer_length_fw":23,"gc_clamp_fw":1,"gc_ratio_fw":0.54348,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":60.96,"Self_Dimer_DeltaG":-1.22,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":3,"Number_of_mismatches_hexamer":1,"_row":"56_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH3","Template":"IGHV1-8","Group":"IGHV1-8","Number_of_mismatches":7,"primer_efficiency":0.0006,"annealing_DeltaG":-5.255,"Position_3terminus":9,"All_mismatches":"23,21,17,13,11,10,9","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0","Primer_Sequence":"aaggtgtccagtgtgargtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":23,"gc_clamp_fw":1,"gc_ratio_fw":0.54348,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":60.96,"Self_Dimer_DeltaG":-1.22,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"57_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH3","Template":"IGHV2-26","Group":"IGHV2-26","Number_of_mismatches":12,"primer_efficiency":0,"annealing_DeltaG":-2.388,"Position_3terminus":2,"All_mismatches":"23,18,17,14,13,12,11,10,8,4,3,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"aaggtgtccagtgtgargtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Amplified","primer_length_fw":23,"gc_clamp_fw":1,"gc_ratio_fw":0.54348,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":60.96,"Self_Dimer_DeltaG":-1.22,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":3,"_row":"58_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH3","Template":"IGHV2-5","Group":"IGHV2-5","Number_of_mismatches":12,"primer_efficiency":0,"annealing_DeltaG":-1.535,"Position_3terminus":1,"All_mismatches":"19,15,14,11,10,9,7,5,4,3,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"aaggtgtccagtgtgargtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":5,"Experimental_Coverage":"Unamplified","primer_length_fw":23,"gc_clamp_fw":1,"gc_ratio_fw":0.54348,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":60.96,"Self_Dimer_DeltaG":-1.22,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":5,"_row":"59_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH3","Template":"IGHV2-70","Group":"IGHV2-70","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-1.703,"Position_3terminus":1,"All_mismatches":"23,22,21,19,16,13,12,10,8,3,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"aaggtgtccagtgtgargtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":23,"gc_clamp_fw":1,"gc_ratio_fw":0.54348,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":60.96,"Self_Dimer_DeltaG":-1.22,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":2,"_row":"60_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH3","Template":"IGHV3-11","Group":"IGHV3-11","Number_of_mismatches":1,"primer_efficiency":0.99692,"annealing_DeltaG":-14.016,"Position_3terminus":9,"All_mismatches":"9","Worst_Case_MismatchLocal":"0","Primer_Sequence":"aaggtgtccagtgtgargtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":23,"gc_clamp_fw":1,"gc_ratio_fw":0.54348,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":60.96,"Self_Dimer_DeltaG":-1.22,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"61_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH3","Template":"IGHV3-13","Group":"IGHV3-13","Number_of_mismatches":0,"primer_efficiency":0.99994,"annealing_DeltaG":-16.616,"All_mismatches":"NA","Worst_Case_MismatchLocal":"0","Primer_Sequence":"aaggtgtccagtgtgargtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":23,"gc_clamp_fw":1,"gc_ratio_fw":0.54348,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":60.96,"Self_Dimer_DeltaG":-1.22,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"62_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH3","Template":"IGHV3-15","Group":"IGHV3-15","Number_of_mismatches":0,"primer_efficiency":0.99994,"annealing_DeltaG":-16.616,"All_mismatches":"NA","Worst_Case_MismatchLocal":"0","Primer_Sequence":"aaggtgtccagtgtgargtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":23,"gc_clamp_fw":1,"gc_ratio_fw":0.54348,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":60.96,"Self_Dimer_DeltaG":-1.22,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"63_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH3","Template":"IGHV3-20","Group":"IGHV3-20","Number_of_mismatches":0,"primer_efficiency":0.99994,"annealing_DeltaG":-16.616,"All_mismatches":"NA","Worst_Case_MismatchLocal":"0","Primer_Sequence":"aaggtgtccagtgtgargtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":23,"gc_clamp_fw":1,"gc_ratio_fw":0.54348,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":60.96,"Self_Dimer_DeltaG":-1.22,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"64_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH3","Template":"IGHV3-21","Group":"IGHV3-21","Number_of_mismatches":0,"primer_efficiency":0.99994,"annealing_DeltaG":-16.616,"All_mismatches":"NA","Worst_Case_MismatchLocal":"0","Primer_Sequence":"aaggtgtccagtgtgargtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":23,"gc_clamp_fw":1,"gc_ratio_fw":0.54348,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":60.96,"Self_Dimer_DeltaG":-1.22,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"65_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH3","Template":"IGHV3-23","Group":"IGHV3-23","Number_of_mismatches":0,"primer_efficiency":0.99994,"annealing_DeltaG":-16.616,"All_mismatches":"NA","Worst_Case_MismatchLocal":"0","Primer_Sequence":"aaggtgtccagtgtgargtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":23,"gc_clamp_fw":1,"gc_ratio_fw":0.54348,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":60.96,"Self_Dimer_DeltaG":-1.22,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"66_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH3","Template":"IGHV3-30","Group":"IGHV3-30","Number_of_mismatches":2,"primer_efficiency":0.99687,"annealing_DeltaG":-14.006,"Position_3terminus":9,"All_mismatches":"23,9","Worst_Case_MismatchLocal":"0,0","Primer_Sequence":"aaggtgtccagtgtgargtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":23,"gc_clamp_fw":1,"gc_ratio_fw":0.54348,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":60.96,"Self_Dimer_DeltaG":-1.22,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"67_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH3","Template":"IGHV3-30-3","Group":"IGHV3-30-3","Number_of_mismatches":2,"primer_efficiency":0.99687,"annealing_DeltaG":-14.006,"Position_3terminus":9,"All_mismatches":"23,9","Worst_Case_MismatchLocal":"0,0","Primer_Sequence":"aaggtgtccagtgtgargtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":23,"gc_clamp_fw":1,"gc_ratio_fw":0.54348,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":60.96,"Self_Dimer_DeltaG":-1.22,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"68_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH3","Template":"IGHV3-33","Group":"IGHV3-33","Number_of_mismatches":2,"primer_efficiency":0.99687,"annealing_DeltaG":-14.006,"Position_3terminus":9,"All_mismatches":"23,9","Worst_Case_MismatchLocal":"0,0","Primer_Sequence":"aaggtgtccagtgtgargtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":23,"gc_clamp_fw":1,"gc_ratio_fw":0.54348,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":60.96,"Self_Dimer_DeltaG":-1.22,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"69_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH3","Template":"IGHV3-43","Group":"IGHV3-43","Number_of_mismatches":0,"primer_efficiency":0.99979,"annealing_DeltaG":-15.728,"All_mismatches":"NA","Worst_Case_MismatchLocal":"0","Primer_Sequence":"aaggtgtccagtgtgargtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":23,"gc_clamp_fw":1,"gc_ratio_fw":0.54348,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":60.96,"Self_Dimer_DeltaG":-1.22,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"70_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH3","Template":"IGHV3-48","Group":"IGHV3-48","Number_of_mismatches":0,"primer_efficiency":0.99994,"annealing_DeltaG":-16.616,"All_mismatches":"NA","Worst_Case_MismatchLocal":"0","Primer_Sequence":"aaggtgtccagtgtgargtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":23,"gc_clamp_fw":1,"gc_ratio_fw":0.54348,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":60.96,"Self_Dimer_DeltaG":-1.22,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"71_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH3","Template":"IGHV3-49","Group":"IGHV3-49","Number_of_mismatches":1,"primer_efficiency":0.99597,"annealing_DeltaG":-13.838,"Position_3terminus":13,"All_mismatches":"13","Worst_Case_MismatchLocal":"0","Primer_Sequence":"aaggtgtccagtgtgargtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":23,"gc_clamp_fw":1,"gc_ratio_fw":0.54348,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":60.96,"Self_Dimer_DeltaG":-1.22,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"72_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH3","Template":"IGHV3-53","Group":"IGHV3-53","Number_of_mismatches":0,"primer_efficiency":0.99994,"annealing_DeltaG":-16.616,"All_mismatches":"NA","Worst_Case_MismatchLocal":"0","Primer_Sequence":"aaggtgtccagtgtgargtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":23,"gc_clamp_fw":1,"gc_ratio_fw":0.54348,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":60.96,"Self_Dimer_DeltaG":-1.22,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"73_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH3","Template":"IGHV3-64","Group":"IGHV3-64","Number_of_mismatches":0,"primer_efficiency":0.99994,"annealing_DeltaG":-16.616,"All_mismatches":"NA","Worst_Case_MismatchLocal":"0","Primer_Sequence":"aaggtgtccagtgtgargtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":23,"gc_clamp_fw":1,"gc_ratio_fw":0.54348,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":60.96,"Self_Dimer_DeltaG":-1.22,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"74_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH3","Template":"IGHV3-64D","Group":"IGHV3-64D","Number_of_mismatches":1,"primer_efficiency":0.99718,"annealing_DeltaG":-14.074,"Position_3terminus":20,"All_mismatches":"20","Worst_Case_MismatchLocal":"0","Primer_Sequence":"aaggtgtccagtgtgargtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":23,"gc_clamp_fw":1,"gc_ratio_fw":0.54348,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":60.96,"Self_Dimer_DeltaG":-1.22,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"75_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH3","Template":"IGHV3-66","Group":"IGHV3-66","Number_of_mismatches":0,"primer_efficiency":0.99994,"annealing_DeltaG":-16.616,"All_mismatches":"NA","Worst_Case_MismatchLocal":"0","Primer_Sequence":"aaggtgtccagtgtgargtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":23,"gc_clamp_fw":1,"gc_ratio_fw":0.54348,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":60.96,"Self_Dimer_DeltaG":-1.22,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"76_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH3","Template":"IGHV3-7","Group":"IGHV3-7","Number_of_mismatches":0,"primer_efficiency":0.99994,"annealing_DeltaG":-16.616,"All_mismatches":"NA","Worst_Case_MismatchLocal":"0","Primer_Sequence":"aaggtgtccagtgtgargtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":23,"gc_clamp_fw":1,"gc_ratio_fw":0.54348,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":60.96,"Self_Dimer_DeltaG":-1.22,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"77_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH3","Template":"IGHV3-72","Group":"IGHV3-72","Number_of_mismatches":0,"primer_efficiency":0.99994,"annealing_DeltaG":-16.616,"All_mismatches":"NA","Worst_Case_MismatchLocal":"0","Primer_Sequence":"aaggtgtccagtgtgargtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":23,"gc_clamp_fw":1,"gc_ratio_fw":0.54348,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":60.96,"Self_Dimer_DeltaG":-1.22,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"78_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH3","Template":"IGHV3-73","Group":"IGHV3-73","Number_of_mismatches":0,"primer_efficiency":0.99994,"annealing_DeltaG":-16.616,"All_mismatches":"NA","Worst_Case_MismatchLocal":"0","Primer_Sequence":"aaggtgtccagtgtgargtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":23,"gc_clamp_fw":1,"gc_ratio_fw":0.54348,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":60.96,"Self_Dimer_DeltaG":-1.22,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"79_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH3","Template":"IGHV3-74","Group":"IGHV3-74","Number_of_mismatches":0,"primer_efficiency":0.99994,"annealing_DeltaG":-16.616,"All_mismatches":"NA","Worst_Case_MismatchLocal":"0","Primer_Sequence":"aaggtgtccagtgtgargtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":23,"gc_clamp_fw":1,"gc_ratio_fw":0.54348,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":60.96,"Self_Dimer_DeltaG":-1.22,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"80_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH3","Template":"IGHV3-9","Group":"IGHV3-9","Number_of_mismatches":0,"primer_efficiency":0.99979,"annealing_DeltaG":-15.728,"All_mismatches":"NA","Worst_Case_MismatchLocal":"0","Primer_Sequence":"aaggtgtccagtgtgargtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":23,"gc_clamp_fw":1,"gc_ratio_fw":0.54348,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":60.96,"Self_Dimer_DeltaG":-1.22,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"81_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH3","Template":"IGHV4-28","Group":"IGHV4-28","Number_of_mismatches":8,"primer_efficiency":2.3429e-06,"annealing_DeltaG":-2.387,"Position_3terminus":4,"All_mismatches":"23,21,19,14,11,10,9,4","Worst_Case_MismatchLocal":"3,3,3,3,3,3,3,3","Primer_Sequence":"aaggtgtccagtgtgargtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Unamplified","primer_length_fw":23,"gc_clamp_fw":1,"gc_ratio_fw":0.54348,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":60.96,"Self_Dimer_DeltaG":-1.22,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":3,"Number_of_mismatches_hexamer":1,"_row":"82_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH3","Template":"IGHV4-30-2","Group":"IGHV4-30-2","Number_of_mismatches":8,"primer_efficiency":0.00004,"annealing_DeltaG":-3.515,"Position_3terminus":6,"All_mismatches":"23,21,19,14,11,10,9,6","Worst_Case_MismatchLocal":"1,1,1,1,1,1,1,1","Primer_Sequence":"aaggtgtccagtgtgargtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Amplified","primer_length_fw":23,"gc_clamp_fw":1,"gc_ratio_fw":0.54348,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":60.96,"Self_Dimer_DeltaG":-1.22,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":1,"Number_of_mismatches_hexamer":1,"_row":"83_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH3","Template":"IGHV4-31","Group":"IGHV4-31","Number_of_mismatches":7,"primer_efficiency":0.0006,"annealing_DeltaG":-5.255,"Position_3terminus":9,"All_mismatches":"23,21,19,14,11,10,9","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0","Primer_Sequence":"aaggtgtccagtgtgargtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":23,"gc_clamp_fw":1,"gc_ratio_fw":0.54348,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":60.96,"Self_Dimer_DeltaG":-1.22,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"84_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH3","Template":"IGHV4-34","Group":"IGHV4-34","Number_of_mismatches":7,"primer_efficiency":0.0006,"annealing_DeltaG":-5.255,"Position_3terminus":9,"All_mismatches":"23,21,19,14,11,10,9","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0","Primer_Sequence":"aaggtgtccagtgtgargtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":23,"gc_clamp_fw":1,"gc_ratio_fw":0.54348,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":60.96,"Self_Dimer_DeltaG":-1.22,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"85_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH3","Template":"IGHV4-38-2","Group":"IGHV4-38-2","Number_of_mismatches":7,"primer_efficiency":0.0006,"annealing_DeltaG":-5.255,"Position_3terminus":9,"All_mismatches":"23,21,19,14,11,10,9","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0","Primer_Sequence":"aaggtgtccagtgtgargtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":23,"gc_clamp_fw":1,"gc_ratio_fw":0.54348,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":60.96,"Self_Dimer_DeltaG":-1.22,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"86_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH3","Template":"IGHV4-39","Group":"IGHV4-39","Number_of_mismatches":8,"primer_efficiency":0.00004,"annealing_DeltaG":-3.515,"Position_3terminus":6,"All_mismatches":"23,21,19,14,11,10,9,6","Worst_Case_MismatchLocal":"1,1,1,1,1,1,1,1","Primer_Sequence":"aaggtgtccagtgtgargtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Amplified","primer_length_fw":23,"gc_clamp_fw":1,"gc_ratio_fw":0.54348,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":60.96,"Self_Dimer_DeltaG":-1.22,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":1,"Number_of_mismatches_hexamer":1,"_row":"87_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH3","Template":"IGHV4-4","Group":"IGHV4-4","Number_of_mismatches":7,"primer_efficiency":0.0006,"annealing_DeltaG":-5.255,"Position_3terminus":9,"All_mismatches":"23,21,19,14,11,10,9","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0","Primer_Sequence":"aaggtgtccagtgtgargtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":23,"gc_clamp_fw":1,"gc_ratio_fw":0.54348,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":60.96,"Self_Dimer_DeltaG":-1.22,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"88_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH3","Template":"IGHV4-59","Group":"IGHV4-59","Number_of_mismatches":7,"primer_efficiency":0.0006,"annealing_DeltaG":-5.255,"Position_3terminus":9,"All_mismatches":"23,21,19,14,11,10,9","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0","Primer_Sequence":"aaggtgtccagtgtgargtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":23,"gc_clamp_fw":1,"gc_ratio_fw":0.54348,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":60.96,"Self_Dimer_DeltaG":-1.22,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"89_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH3","Template":"IGHV4-61","Group":"IGHV4-61","Number_of_mismatches":7,"primer_efficiency":0.0006,"annealing_DeltaG":-5.255,"Position_3terminus":9,"All_mismatches":"23,21,19,14,11,10,9","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0","Primer_Sequence":"aaggtgtccagtgtgargtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":23,"gc_clamp_fw":1,"gc_ratio_fw":0.54348,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":60.96,"Self_Dimer_DeltaG":-1.22,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"90_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH3","Template":"IGHV5-10-1","Group":"IGHV5-10-1","Number_of_mismatches":7,"primer_efficiency":0.00017,"annealing_DeltaG":-4.423,"Position_3terminus":10,"All_mismatches":"19,15,14,13,12,11,10","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0","Primer_Sequence":"aaggtgtccagtgtgargtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":23,"gc_clamp_fw":1,"gc_ratio_fw":0.54348,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":60.96,"Self_Dimer_DeltaG":-1.22,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"91_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH3","Template":"IGHV5-51","Group":"IGHV5-51","Number_of_mismatches":7,"primer_efficiency":0.00066,"annealing_DeltaG":-5.311,"Position_3terminus":10,"All_mismatches":"19,15,14,13,12,11,10","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0","Primer_Sequence":"aaggtgtccagtgtgargtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":23,"gc_clamp_fw":1,"gc_ratio_fw":0.54348,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":60.96,"Self_Dimer_DeltaG":-1.22,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"92_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH3","Template":"IGHV6-1","Group":"IGHV6-1","Number_of_mismatches":7,"primer_efficiency":0.00005,"annealing_DeltaG":-4.382,"Position_3terminus":4,"All_mismatches":"23,22,14,11,10,9,4","Worst_Case_MismatchLocal":"3,3,3,3,3,3,3","Primer_Sequence":"aaggtgtccagtgtgargtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Amplified","primer_length_fw":23,"gc_clamp_fw":1,"gc_ratio_fw":0.54348,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":60.96,"Self_Dimer_DeltaG":-1.22,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":3,"Number_of_mismatches_hexamer":1,"_row":"93_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH3","Template":"IGHV7-4-1","Group":"IGHV7-4-1","Number_of_mismatches":6,"primer_efficiency":0.0006,"annealing_DeltaG":-5.255,"Position_3terminus":9,"All_mismatches":"23,17,13,11,10,9","Worst_Case_MismatchLocal":"0,0,0,0,0,0","Primer_Sequence":"aaggtgtccagtgtgargtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":23,"gc_clamp_fw":1,"gc_ratio_fw":0.54348,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":60.96,"Self_Dimer_DeltaG":-1.22,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"94_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH4_6","Template":"IGHV1-18","Group":"IGHV1-18","Number_of_mismatches":9,"primer_efficiency":0.00037,"annealing_DeltaG":-5.702,"Position_3terminus":4,"All_mismatches":"27,25,23,21,19,17,14,13,4","Worst_Case_MismatchLocal":"3,3,3,3,3,3,3,3,3","Primer_Sequence":"cccagatgggtcctgtcccaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Amplified","primer_length_fw":27,"gc_clamp_fw":1,"gc_ratio_fw":0.66667,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":69,"Self_Dimer_DeltaG":-1.308,"Structure_deltaG_fw":-2.58,"primer_coverage":47,"primer_specificity":0.79661,"Run":"Tiller2008_1st","Position_3terminusLocal":3,"Number_of_mismatches_hexamer":1,"_row":"95_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH4_6","Template":"IGHV1-2","Group":"IGHV1-2","Number_of_mismatches":7,"primer_efficiency":0.02959,"annealing_DeltaG":-8.603,"Position_3terminus":13,"All_mismatches":"27,23,21,19,17,14,13","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0","Primer_Sequence":"cccagatgggtcctgtcccaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":27,"gc_clamp_fw":1,"gc_ratio_fw":0.66667,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":69,"Self_Dimer_DeltaG":-1.308,"Structure_deltaG_fw":-2.58,"primer_coverage":47,"primer_specificity":0.79661,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"96_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH4_6","Template":"IGHV1-24","Group":"IGHV1-24","Number_of_mismatches":11,"primer_efficiency":0.00033,"annealing_DeltaG":-5.64,"Position_3terminus":4,"All_mismatches":"27,25,23,21,19,18,17,14,13,12,4","Worst_Case_MismatchLocal":"3,3,3,3,3,3,3,3,3,3,3","Primer_Sequence":"cccagatgggtcctgtcccaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Amplified","primer_length_fw":27,"gc_clamp_fw":1,"gc_ratio_fw":0.66667,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":69,"Self_Dimer_DeltaG":-1.308,"Structure_deltaG_fw":-2.58,"primer_coverage":47,"primer_specificity":0.79661,"Run":"Tiller2008_1st","Position_3terminusLocal":3,"Number_of_mismatches_hexamer":1,"_row":"97_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH4_6","Template":"IGHV1-3","Group":"IGHV1-3","Number_of_mismatches":8,"primer_efficiency":0.00085,"annealing_DeltaG":-6.252,"Position_3terminus":4,"All_mismatches":"27,23,21,19,17,14,13,4","Worst_Case_MismatchLocal":"3,3,3,3,3,3,3,3","Primer_Sequence":"cccagatgggtcctgtcccaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Amplified","primer_length_fw":27,"gc_clamp_fw":1,"gc_ratio_fw":0.66667,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":69,"Self_Dimer_DeltaG":-1.308,"Structure_deltaG_fw":-2.58,"primer_coverage":47,"primer_specificity":0.79661,"Run":"Tiller2008_1st","Position_3terminusLocal":3,"Number_of_mismatches_hexamer":1,"_row":"98_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH4_6","Template":"IGHV1-45","Group":"IGHV1-45","Number_of_mismatches":10,"primer_efficiency":0.00103,"annealing_DeltaG":-6.061,"Position_3terminus":6,"All_mismatches":"27,23,21,20,19,17,15,14,13,6","Worst_Case_MismatchLocal":"1,1,1,1,1,1,1,1,1,1","Primer_Sequence":"cccagatgggtcctgtcccaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Amplified","primer_length_fw":27,"gc_clamp_fw":1,"gc_ratio_fw":0.66667,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":69,"Self_Dimer_DeltaG":-1.308,"Structure_deltaG_fw":-2.58,"primer_coverage":47,"primer_specificity":0.79661,"Run":"Tiller2008_1st","Position_3terminusLocal":1,"Number_of_mismatches_hexamer":1,"_row":"99_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH4_6","Template":"IGHV1-46","Group":"IGHV1-46","Number_of_mismatches":10,"primer_efficiency":0.04068,"annealing_DeltaG":-8.603,"Position_3terminus":13,"All_mismatches":"27,25,24,23,21,19,17,16,14,13","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0,0,0,0","Primer_Sequence":"cccagatgggtcctgtcccaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":27,"gc_clamp_fw":1,"gc_ratio_fw":0.66667,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":69,"Self_Dimer_DeltaG":-1.308,"Structure_deltaG_fw":-2.58,"primer_coverage":47,"primer_specificity":0.79661,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"100_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH4_6","Template":"IGHV1-58","Group":"IGHV1-58","Number_of_mismatches":10,"primer_efficiency":0.00019,"annealing_DeltaG":-4.499,"Position_3terminus":6,"All_mismatches":"27,25,23,21,19,17,14,13,7,6","Worst_Case_MismatchLocal":"1,1,1,1,1,1,1,1,1,1","Primer_Sequence":"cccagatgggtcctgtcccaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Amplified","primer_length_fw":27,"gc_clamp_fw":1,"gc_ratio_fw":0.66667,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":69,"Self_Dimer_DeltaG":-1.308,"Structure_deltaG_fw":-2.58,"primer_coverage":47,"primer_specificity":0.79661,"Run":"Tiller2008_1st","Position_3terminusLocal":1,"Number_of_mismatches_hexamer":1,"_row":"101_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH4_6","Template":"IGHV1-69","Group":"IGHV1-69","Number_of_mismatches":6,"primer_efficiency":0.80075,"annealing_DeltaG":-11.514,"Position_3terminus":14,"All_mismatches":"27,25,23,21,19,14","Worst_Case_MismatchLocal":"0,0,0,0,0,0","Primer_Sequence":"cccagatgggtcctgtcccaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":27,"gc_clamp_fw":1,"gc_ratio_fw":0.66667,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":69,"Self_Dimer_DeltaG":-1.308,"Structure_deltaG_fw":-2.58,"primer_coverage":47,"primer_specificity":0.79661,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"102_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH4_6","Template":"IGHV1-69-2","Group":"IGHV1-69-2","Number_of_mismatches":12,"primer_efficiency":0,"annealing_DeltaG":-2.857,"Position_3terminus":4,"All_mismatches":"27,25,23,21,19,18,17,14,13,12,9,4","Worst_Case_MismatchLocal":"3,3,3,3,3,3,3,3,3,3,3,3","Primer_Sequence":"cccagatgggtcctgtcccaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Unamplified","primer_length_fw":27,"gc_clamp_fw":1,"gc_ratio_fw":0.66667,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":69,"Self_Dimer_DeltaG":-1.308,"Structure_deltaG_fw":-2.58,"primer_coverage":47,"primer_specificity":0.79661,"Run":"Tiller2008_1st","Position_3terminusLocal":3,"Number_of_mismatches_hexamer":1,"_row":"103_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH4_6","Template":"IGHV1-8","Group":"IGHV1-8","Number_of_mismatches":8,"primer_efficiency":0.03777,"annealing_DeltaG":-8.603,"Position_3terminus":13,"All_mismatches":"27,25,23,21,19,17,14,13","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0,0","Primer_Sequence":"cccagatgggtcctgtcccaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":27,"gc_clamp_fw":1,"gc_ratio_fw":0.66667,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":69,"Self_Dimer_DeltaG":-1.308,"Structure_deltaG_fw":-2.58,"primer_coverage":47,"primer_specificity":0.79661,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"104_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH4_6","Template":"IGHV2-26","Group":"IGHV2-26","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-9.953,"Position_3terminus":1,"All_mismatches":"25,24,23,22,15,4,3,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6","Primer_Sequence":"cccagatgggtcctgtcccaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Amplified","primer_length_fw":27,"gc_clamp_fw":1,"gc_ratio_fw":0.66667,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":69,"Self_Dimer_DeltaG":-1.308,"Structure_deltaG_fw":-2.58,"primer_coverage":47,"primer_specificity":0.79661,"Run":"Tiller2008_1st","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":4,"_row":"105_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH4_6","Template":"IGHV2-5","Group":"IGHV2-5","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-8.243,"Position_3terminus":1,"All_mismatches":"25,24,23,15,6,4,3,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6","Primer_Sequence":"cccagatgggtcctgtcccaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":5,"Experimental_Coverage":"Unamplified","primer_length_fw":27,"gc_clamp_fw":1,"gc_ratio_fw":0.66667,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":69,"Self_Dimer_DeltaG":-1.308,"Structure_deltaG_fw":-2.58,"primer_coverage":47,"primer_specificity":0.79661,"Run":"Tiller2008_1st","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":5,"_row":"106_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH4_6","Template":"IGHV2-70","Group":"IGHV2-70","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-7.266,"Position_3terminus":1,"All_mismatches":"25,24,23,22,15,13,4,3,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"cccagatgggtcctgtcccaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":27,"gc_clamp_fw":1,"gc_ratio_fw":0.66667,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":69,"Self_Dimer_DeltaG":-1.308,"Structure_deltaG_fw":-2.58,"primer_coverage":47,"primer_specificity":0.79661,"Run":"Tiller2008_1st","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":4,"_row":"107_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH4_6","Template":"IGHV3-11","Group":"IGHV3-11","Number_of_mismatches":9,"primer_efficiency":0.00099,"annealing_DeltaG":-6.047,"Position_3terminus":10,"All_mismatches":"27,26,25,23,21,19,14,11,10","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0,0,0","Primer_Sequence":"cccagatgggtcctgtcccaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":27,"gc_clamp_fw":1,"gc_ratio_fw":0.66667,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":69,"Self_Dimer_DeltaG":-1.308,"Structure_deltaG_fw":-2.58,"primer_coverage":47,"primer_specificity":0.79661,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"108_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH4_6","Template":"IGHV3-13","Group":"IGHV3-13","Number_of_mismatches":11,"primer_efficiency":0.00056,"annealing_DeltaG":-5.208,"Position_3terminus":9,"All_mismatches":"27,26,25,24,23,21,19,14,11,10,9","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0,0,0,0,0","Primer_Sequence":"cccagatgggtcctgtcccaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":27,"gc_clamp_fw":1,"gc_ratio_fw":0.66667,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":69,"Self_Dimer_DeltaG":-1.308,"Structure_deltaG_fw":-2.58,"primer_coverage":47,"primer_specificity":0.79661,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"109_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH4_6","Template":"IGHV3-15","Group":"IGHV3-15","Number_of_mismatches":10,"primer_efficiency":0.00056,"annealing_DeltaG":-5.208,"Position_3terminus":9,"All_mismatches":"27,26,25,23,21,19,14,11,10,9","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0,0,0,0","Primer_Sequence":"cccagatgggtcctgtcccaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":27,"gc_clamp_fw":1,"gc_ratio_fw":0.66667,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":69,"Self_Dimer_DeltaG":-1.308,"Structure_deltaG_fw":-2.58,"primer_coverage":47,"primer_specificity":0.79661,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"110_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH4_6","Template":"IGHV3-20","Group":"IGHV3-20","Number_of_mismatches":10,"primer_efficiency":0.00056,"annealing_DeltaG":-5.208,"Position_3terminus":9,"All_mismatches":"27,26,25,23,21,19,14,11,10,9","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0,0,0,0","Primer_Sequence":"cccagatgggtcctgtcccaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":27,"gc_clamp_fw":1,"gc_ratio_fw":0.66667,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":69,"Self_Dimer_DeltaG":-1.308,"Structure_deltaG_fw":-2.58,"primer_coverage":47,"primer_specificity":0.79661,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"111_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH4_6","Template":"IGHV3-21","Group":"IGHV3-21","Number_of_mismatches":11,"primer_efficiency":0.00056,"annealing_DeltaG":-5.208,"Position_3terminus":9,"All_mismatches":"27,26,25,24,23,21,19,14,11,10,9","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0,0,0,0,0","Primer_Sequence":"cccagatgggtcctgtcccaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":27,"gc_clamp_fw":1,"gc_ratio_fw":0.66667,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":69,"Self_Dimer_DeltaG":-1.308,"Structure_deltaG_fw":-2.58,"primer_coverage":47,"primer_specificity":0.79661,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"112_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH4_6","Template":"IGHV3-23","Group":"IGHV3-23","Number_of_mismatches":10,"primer_efficiency":0.00056,"annealing_DeltaG":-5.208,"Position_3terminus":9,"All_mismatches":"27,26,25,23,21,19,14,11,10,9","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0,0,0,0","Primer_Sequence":"cccagatgggtcctgtcccaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":27,"gc_clamp_fw":1,"gc_ratio_fw":0.66667,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":69,"Self_Dimer_DeltaG":-1.308,"Structure_deltaG_fw":-2.58,"primer_coverage":47,"primer_specificity":0.79661,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"113_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH4_6","Template":"IGHV3-30","Group":"IGHV3-30","Number_of_mismatches":8,"primer_efficiency":0.00099,"annealing_DeltaG":-6.047,"Position_3terminus":10,"All_mismatches":"27,26,25,21,19,14,11,10","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0,0","Primer_Sequence":"cccagatgggtcctgtcccaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":27,"gc_clamp_fw":1,"gc_ratio_fw":0.66667,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":69,"Self_Dimer_DeltaG":-1.308,"Structure_deltaG_fw":-2.58,"primer_coverage":47,"primer_specificity":0.79661,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"114_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH4_6","Template":"IGHV3-30-3","Group":"IGHV3-30-3","Number_of_mismatches":8,"primer_efficiency":0.00099,"annealing_DeltaG":-6.047,"Position_3terminus":10,"All_mismatches":"27,26,25,21,19,14,11,10","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0,0","Primer_Sequence":"cccagatgggtcctgtcccaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":27,"gc_clamp_fw":1,"gc_ratio_fw":0.66667,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":69,"Self_Dimer_DeltaG":-1.308,"Structure_deltaG_fw":-2.58,"primer_coverage":47,"primer_specificity":0.79661,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"115_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH4_6","Template":"IGHV3-33","Group":"IGHV3-33","Number_of_mismatches":8,"primer_efficiency":0.00099,"annealing_DeltaG":-6.047,"Position_3terminus":10,"All_mismatches":"27,26,25,21,19,14,11,10","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0,0","Primer_Sequence":"cccagatgggtcctgtcccaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":27,"gc_clamp_fw":1,"gc_ratio_fw":0.66667,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":69,"Self_Dimer_DeltaG":-1.308,"Structure_deltaG_fw":-2.58,"primer_coverage":47,"primer_specificity":0.79661,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"116_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH4_6","Template":"IGHV3-43","Group":"IGHV3-43","Number_of_mismatches":11,"primer_efficiency":0.00002,"annealing_DeltaG":-2.877,"Position_3terminus":7,"All_mismatches":"27,26,25,23,21,19,14,11,10,9,7","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0,0,0,0,0","Primer_Sequence":"cccagatgggtcctgtcccaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Unamplified","primer_length_fw":27,"gc_clamp_fw":1,"gc_ratio_fw":0.66667,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":69,"Self_Dimer_DeltaG":-1.308,"Structure_deltaG_fw":-2.58,"primer_coverage":47,"primer_specificity":0.79661,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"117_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH4_6","Template":"IGHV3-48","Group":"IGHV3-48","Number_of_mismatches":11,"primer_efficiency":0.00056,"annealing_DeltaG":-5.208,"Position_3terminus":9,"All_mismatches":"27,26,25,24,23,21,19,14,11,10,9","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0,0,0,0,0","Primer_Sequence":"cccagatgggtcctgtcccaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":27,"gc_clamp_fw":1,"gc_ratio_fw":0.66667,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":69,"Self_Dimer_DeltaG":-1.308,"Structure_deltaG_fw":-2.58,"primer_coverage":47,"primer_specificity":0.79661,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"118_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH4_6","Template":"IGHV3-49","Group":"IGHV3-49","Number_of_mismatches":11,"primer_efficiency":0.00056,"annealing_DeltaG":-5.208,"Position_3terminus":9,"All_mismatches":"27,26,25,23,21,19,14,13,11,10,9","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0,0,0,0,0","Primer_Sequence":"cccagatgggtcctgtcccaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":27,"gc_clamp_fw":1,"gc_ratio_fw":0.66667,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":69,"Self_Dimer_DeltaG":-1.308,"Structure_deltaG_fw":-2.58,"primer_coverage":47,"primer_specificity":0.79661,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"119_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH4_6","Template":"IGHV3-53","Group":"IGHV3-53","Number_of_mismatches":9,"primer_efficiency":0.00056,"annealing_DeltaG":-5.208,"Position_3terminus":9,"All_mismatches":"27,25,23,21,19,14,11,10,9","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0,0,0","Primer_Sequence":"cccagatgggtcctgtcccaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":27,"gc_clamp_fw":1,"gc_ratio_fw":0.66667,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":69,"Self_Dimer_DeltaG":-1.308,"Structure_deltaG_fw":-2.58,"primer_coverage":47,"primer_specificity":0.79661,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"120_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH4_6","Template":"IGHV3-64","Group":"IGHV3-64","Number_of_mismatches":10,"primer_efficiency":0.00056,"annealing_DeltaG":-5.208,"Position_3terminus":9,"All_mismatches":"27,26,25,23,21,19,14,11,10,9","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0,0,0,0","Primer_Sequence":"cccagatgggtcctgtcccaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":27,"gc_clamp_fw":1,"gc_ratio_fw":0.66667,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":69,"Self_Dimer_DeltaG":-1.308,"Structure_deltaG_fw":-2.58,"primer_coverage":47,"primer_specificity":0.79661,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"121_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH4_6","Template":"IGHV3-64D","Group":"IGHV3-64D","Number_of_mismatches":11,"primer_efficiency":0.00056,"annealing_DeltaG":-5.208,"Position_3terminus":9,"All_mismatches":"27,26,25,23,21,20,19,14,11,10,9","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0,0,0,0,0","Primer_Sequence":"cccagatgggtcctgtcccaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":27,"gc_clamp_fw":1,"gc_ratio_fw":0.66667,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":69,"Self_Dimer_DeltaG":-1.308,"Structure_deltaG_fw":-2.58,"primer_coverage":47,"primer_specificity":0.79661,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"122_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH4_6","Template":"IGHV3-66","Group":"IGHV3-66","Number_of_mismatches":10,"primer_efficiency":0.00056,"annealing_DeltaG":-5.208,"Position_3terminus":9,"All_mismatches":"27,26,25,23,21,19,14,11,10,9","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0,0,0,0","Primer_Sequence":"cccagatgggtcctgtcccaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":27,"gc_clamp_fw":1,"gc_ratio_fw":0.66667,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":69,"Self_Dimer_DeltaG":-1.308,"Structure_deltaG_fw":-2.58,"primer_coverage":47,"primer_specificity":0.79661,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"123_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH4_6","Template":"IGHV3-7","Group":"IGHV3-7","Number_of_mismatches":11,"primer_efficiency":0.00056,"annealing_DeltaG":-5.208,"Position_3terminus":9,"All_mismatches":"27,26,25,24,23,21,19,14,11,10,9","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0,0,0,0,0","Primer_Sequence":"cccagatgggtcctgtcccaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":27,"gc_clamp_fw":1,"gc_ratio_fw":0.66667,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":69,"Self_Dimer_DeltaG":-1.308,"Structure_deltaG_fw":-2.58,"primer_coverage":47,"primer_specificity":0.79661,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"124_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH4_6","Template":"IGHV3-72","Group":"IGHV3-72","Number_of_mismatches":11,"primer_efficiency":0.00056,"annealing_DeltaG":-5.208,"Position_3terminus":9,"All_mismatches":"27,26,25,24,23,21,19,14,11,10,9","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0,0,0,0,0","Primer_Sequence":"cccagatgggtcctgtcccaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":27,"gc_clamp_fw":1,"gc_ratio_fw":0.66667,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":69,"Self_Dimer_DeltaG":-1.308,"Structure_deltaG_fw":-2.58,"primer_coverage":47,"primer_specificity":0.79661,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"125_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH4_6","Template":"IGHV3-73","Group":"IGHV3-73","Number_of_mismatches":10,"primer_efficiency":0.00056,"annealing_DeltaG":-5.208,"Position_3terminus":9,"All_mismatches":"27,26,25,23,21,19,14,11,10,9","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0,0,0,0","Primer_Sequence":"cccagatgggtcctgtcccaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":27,"gc_clamp_fw":1,"gc_ratio_fw":0.66667,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":69,"Self_Dimer_DeltaG":-1.308,"Structure_deltaG_fw":-2.58,"primer_coverage":47,"primer_specificity":0.79661,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"126_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH4_6","Template":"IGHV3-74","Group":"IGHV3-74","Number_of_mismatches":10,"primer_efficiency":0.00056,"annealing_DeltaG":-5.208,"Position_3terminus":9,"All_mismatches":"27,26,25,23,21,19,14,11,10,9","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0,0,0,0","Primer_Sequence":"cccagatgggtcctgtcccaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":27,"gc_clamp_fw":1,"gc_ratio_fw":0.66667,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":69,"Self_Dimer_DeltaG":-1.308,"Structure_deltaG_fw":-2.58,"primer_coverage":47,"primer_specificity":0.79661,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"127_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH4_6","Template":"IGHV3-9","Group":"IGHV3-9","Number_of_mismatches":11,"primer_efficiency":0.00002,"annealing_DeltaG":-2.877,"Position_3terminus":7,"All_mismatches":"27,26,25,23,21,19,14,11,10,9,7","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0,0,0,0,0","Primer_Sequence":"cccagatgggtcctgtcccaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Unamplified","primer_length_fw":27,"gc_clamp_fw":1,"gc_ratio_fw":0.66667,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":69,"Self_Dimer_DeltaG":-1.308,"Structure_deltaG_fw":-2.58,"primer_coverage":47,"primer_specificity":0.79661,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"128_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH4_6","Template":"IGHV4-28","Group":"IGHV4-28","Number_of_mismatches":1,"primer_efficiency":0.30794,"annealing_DeltaG":-18.326,"Position_3terminus":4,"All_mismatches":"4","Worst_Case_MismatchLocal":"3","Primer_Sequence":"cccagatgggtcctgtcccaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Amplified","primer_length_fw":27,"gc_clamp_fw":1,"gc_ratio_fw":0.66667,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":69,"Self_Dimer_DeltaG":-1.308,"Structure_deltaG_fw":-2.58,"primer_coverage":47,"primer_specificity":0.79661,"Run":"Tiller2008_1st","Position_3terminusLocal":3,"Number_of_mismatches_hexamer":1,"_row":"129_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH4_6","Template":"IGHV4-30-2","Group":"IGHV4-30-2","Number_of_mismatches":1,"primer_efficiency":1,"annealing_DeltaG":-19.454,"Position_3terminus":6,"All_mismatches":"6","Worst_Case_MismatchLocal":"1","Primer_Sequence":"cccagatgggtcctgtcccaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Amplified","primer_length_fw":27,"gc_clamp_fw":1,"gc_ratio_fw":0.66667,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":69,"Self_Dimer_DeltaG":-1.308,"Structure_deltaG_fw":-2.58,"primer_coverage":47,"primer_specificity":0.79661,"Run":"Tiller2008_1st","Position_3terminusLocal":1,"Number_of_mismatches_hexamer":1,"_row":"130_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH4_6","Template":"IGHV4-31","Group":"IGHV4-31","Number_of_mismatches":0,"primer_efficiency":1,"annealing_DeltaG":-21.194,"All_mismatches":"NA","Worst_Case_MismatchLocal":"0","Primer_Sequence":"cccagatgggtcctgtcccaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":27,"gc_clamp_fw":1,"gc_ratio_fw":0.66667,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":69,"Self_Dimer_DeltaG":-1.308,"Structure_deltaG_fw":-2.58,"primer_coverage":47,"primer_specificity":0.79661,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"131_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH4_6","Template":"IGHV4-34","Group":"IGHV4-34","Number_of_mismatches":0,"primer_efficiency":1,"annealing_DeltaG":-21.194,"All_mismatches":"NA","Worst_Case_MismatchLocal":"0","Primer_Sequence":"cccagatgggtcctgtcccaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":27,"gc_clamp_fw":1,"gc_ratio_fw":0.66667,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":69,"Self_Dimer_DeltaG":-1.308,"Structure_deltaG_fw":-2.58,"primer_coverage":47,"primer_specificity":0.79661,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"132_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH4_6","Template":"IGHV4-38-2","Group":"IGHV4-38-2","Number_of_mismatches":0,"primer_efficiency":1,"annealing_DeltaG":-21.194,"All_mismatches":"NA","Worst_Case_MismatchLocal":"0","Primer_Sequence":"cccagatgggtcctgtcccaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":27,"gc_clamp_fw":1,"gc_ratio_fw":0.66667,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":69,"Self_Dimer_DeltaG":-1.308,"Structure_deltaG_fw":-2.58,"primer_coverage":47,"primer_specificity":0.79661,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"133_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH4_6","Template":"IGHV4-39","Group":"IGHV4-39","Number_of_mismatches":1,"primer_efficiency":1,"annealing_DeltaG":-19.454,"Position_3terminus":6,"All_mismatches":"6","Worst_Case_MismatchLocal":"1","Primer_Sequence":"cccagatgggtcctgtcccaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Amplified","primer_length_fw":27,"gc_clamp_fw":1,"gc_ratio_fw":0.66667,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":69,"Self_Dimer_DeltaG":-1.308,"Structure_deltaG_fw":-2.58,"primer_coverage":47,"primer_specificity":0.79661,"Run":"Tiller2008_1st","Position_3terminusLocal":1,"Number_of_mismatches_hexamer":1,"_row":"134_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH4_6","Template":"IGHV4-4","Group":"IGHV4-4","Number_of_mismatches":0,"primer_efficiency":1,"annealing_DeltaG":-21.194,"All_mismatches":"NA","Worst_Case_MismatchLocal":"0","Primer_Sequence":"cccagatgggtcctgtcccaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":27,"gc_clamp_fw":1,"gc_ratio_fw":0.66667,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":69,"Self_Dimer_DeltaG":-1.308,"Structure_deltaG_fw":-2.58,"primer_coverage":47,"primer_specificity":0.79661,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"135_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH4_6","Template":"IGHV4-59","Group":"IGHV4-59","Number_of_mismatches":0,"primer_efficiency":1,"annealing_DeltaG":-21.194,"All_mismatches":"NA","Worst_Case_MismatchLocal":"0","Primer_Sequence":"cccagatgggtcctgtcccaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":27,"gc_clamp_fw":1,"gc_ratio_fw":0.66667,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":69,"Self_Dimer_DeltaG":-1.308,"Structure_deltaG_fw":-2.58,"primer_coverage":47,"primer_specificity":0.79661,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"136_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH4_6","Template":"IGHV4-61","Group":"IGHV4-61","Number_of_mismatches":0,"primer_efficiency":1,"annealing_DeltaG":-21.194,"All_mismatches":"NA","Worst_Case_MismatchLocal":"0","Primer_Sequence":"cccagatgggtcctgtcccaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":27,"gc_clamp_fw":1,"gc_ratio_fw":0.66667,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":69,"Self_Dimer_DeltaG":-1.308,"Structure_deltaG_fw":-2.58,"primer_coverage":47,"primer_specificity":0.79661,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"137_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH4_6","Template":"IGHV5-10-1","Group":"IGHV5-10-1","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-2.827,"Position_3terminus":7,"All_mismatches":"26,24,23,21,19,15,14,13,12,9,7","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0,0,0,0,0","Primer_Sequence":"cccagatgggtcctgtcccaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Unamplified","primer_length_fw":27,"gc_clamp_fw":1,"gc_ratio_fw":0.66667,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":69,"Self_Dimer_DeltaG":-1.308,"Structure_deltaG_fw":-2.58,"primer_coverage":47,"primer_specificity":0.79661,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"138_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH4_6","Template":"IGHV5-51","Group":"IGHV5-51","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-5.208,"Position_3terminus":9,"All_mismatches":"26,24,23,21,19,15,14,13,12,9","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0,0,0,0","Primer_Sequence":"cccagatgggtcctgtcccaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":27,"gc_clamp_fw":1,"gc_ratio_fw":0.66667,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":69,"Self_Dimer_DeltaG":-1.308,"Structure_deltaG_fw":-2.58,"primer_coverage":47,"primer_specificity":0.79661,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"139_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH4_6","Template":"IGHV6-1","Group":"IGHV6-1","Number_of_mismatches":7,"primer_efficiency":0.0003,"annealing_DeltaG":-6.586,"Position_3terminus":4,"All_mismatches":"25,24,22,21,19,10,4","Worst_Case_MismatchLocal":"3,3,3,3,3,3,3","Primer_Sequence":"cccagatgggtcctgtcccaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Amplified","primer_length_fw":27,"gc_clamp_fw":1,"gc_ratio_fw":0.66667,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":69,"Self_Dimer_DeltaG":-1.308,"Structure_deltaG_fw":-2.58,"primer_coverage":47,"primer_specificity":0.79661,"Run":"Tiller2008_1st","Position_3terminusLocal":3,"Number_of_mismatches_hexamer":1,"_row":"140_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH4_6","Template":"IGHV7-4-1","Group":"IGHV7-4-1","Number_of_mismatches":8,"primer_efficiency":0.0291,"annealing_DeltaG":-8.603,"Position_3terminus":13,"All_mismatches":"27,25,23,21,19,17,14,13","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0,0","Primer_Sequence":"cccagatgggtcctgtcccaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":27,"gc_clamp_fw":1,"gc_ratio_fw":0.66667,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":69,"Self_Dimer_DeltaG":-1.308,"Structure_deltaG_fw":-2.58,"primer_coverage":47,"primer_specificity":0.79661,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"141_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH5","Template":"IGHV1-18","Group":"IGHV1-18","Number_of_mismatches":9,"primer_efficiency":0.00005,"annealing_DeltaG":-4.446,"Position_3terminus":4,"All_mismatches":"24,23,19,17,15,14,13,9,4","Worst_Case_MismatchLocal":"3,3,3,3,3,3,3,3,3","Primer_Sequence":"caaggagtctgttccgaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.58333,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":61.38,"Self_Dimer_DeltaG":-0.765,"Structure_deltaG_fw":-0.32,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":3,"Number_of_mismatches_hexamer":1,"_row":"142_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH5","Template":"IGHV1-2","Group":"IGHV1-2","Number_of_mismatches":7,"primer_efficiency":0.00174,"annealing_DeltaG":-6.38,"Position_3terminus":9,"All_mismatches":"24,23,17,15,14,13,9","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0","Primer_Sequence":"caaggagtctgttccgaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.58333,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":61.38,"Self_Dimer_DeltaG":-0.765,"Structure_deltaG_fw":-0.32,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"143_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH5","Template":"IGHV1-24","Group":"IGHV1-24","Number_of_mismatches":10,"primer_efficiency":1.19244e-06,"annealing_DeltaG":-1.725,"Position_3terminus":5,"All_mismatches":"24,23,18,17,14,10,9,8,6,5","Worst_Case_MismatchLocal":"2,2,2,2,2,2,2,2,2,2","Primer_Sequence":"caaggagtctgttccgaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.58333,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":61.38,"Self_Dimer_DeltaG":-0.765,"Structure_deltaG_fw":-0.32,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":2,"Number_of_mismatches_hexamer":2,"_row":"144_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH5","Template":"IGHV1-3","Group":"IGHV1-3","Number_of_mismatches":9,"primer_efficiency":0.00005,"annealing_DeltaG":-4.446,"Position_3terminus":4,"All_mismatches":"24,23,19,17,15,14,13,9,4","Worst_Case_MismatchLocal":"3,3,3,3,3,3,3,3,3","Primer_Sequence":"caaggagtctgttccgaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Unamplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.58333,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":61.38,"Self_Dimer_DeltaG":-0.765,"Structure_deltaG_fw":-0.32,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":3,"Number_of_mismatches_hexamer":1,"_row":"145_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH5","Template":"IGHV1-45","Group":"IGHV1-45","Number_of_mismatches":9,"primer_efficiency":0.00002,"annealing_DeltaG":-3.065,"Position_3terminus":6,"All_mismatches":"24,23,20,19,17,14,13,9,6","Worst_Case_MismatchLocal":"1,1,1,1,1,1,1,1,1","Primer_Sequence":"caaggagtctgttccgaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.58333,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":61.38,"Self_Dimer_DeltaG":-0.765,"Structure_deltaG_fw":-0.32,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":1,"Number_of_mismatches_hexamer":1,"_row":"146_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH5","Template":"IGHV1-46","Group":"IGHV1-46","Number_of_mismatches":8,"primer_efficiency":0.00076,"annealing_DeltaG":-5.607,"Position_3terminus":9,"All_mismatches":"23,19,17,16,15,14,13,9","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0,0","Primer_Sequence":"caaggagtctgttccgaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.58333,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":61.38,"Self_Dimer_DeltaG":-0.765,"Structure_deltaG_fw":-0.32,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"147_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH5","Template":"IGHV1-58","Group":"IGHV1-58","Number_of_mismatches":10,"primer_efficiency":0.00018,"annealing_DeltaG":-4.446,"Position_3terminus":6,"All_mismatches":"24,23,19,17,15,14,13,9,7,6","Worst_Case_MismatchLocal":"1,1,1,1,1,1,1,1,1,1","Primer_Sequence":"caaggagtctgttccgaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.58333,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":61.38,"Self_Dimer_DeltaG":-0.765,"Structure_deltaG_fw":-0.32,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":1,"Number_of_mismatches_hexamer":1,"_row":"148_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH5","Template":"IGHV1-69","Group":"IGHV1-69","Number_of_mismatches":7,"primer_efficiency":0.00086,"annealing_DeltaG":-5.607,"Position_3terminus":9,"All_mismatches":"24,23,19,15,14,13,9","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0","Primer_Sequence":"caaggagtctgttccgaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.58333,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":61.38,"Self_Dimer_DeltaG":-0.765,"Structure_deltaG_fw":-0.32,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"149_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH5","Template":"IGHV1-69-2","Group":"IGHV1-69-2","Number_of_mismatches":10,"primer_efficiency":0.00036,"annealing_DeltaG":-5.683,"Position_3terminus":4,"All_mismatches":"24,23,19,18,17,15,14,13,12,4","Worst_Case_MismatchLocal":"3,3,3,3,3,3,3,3,3,3","Primer_Sequence":"caaggagtctgttccgaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.58333,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":61.38,"Self_Dimer_DeltaG":-0.765,"Structure_deltaG_fw":-0.32,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":3,"Number_of_mismatches_hexamer":1,"_row":"150_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH5","Template":"IGHV1-8","Group":"IGHV1-8","Number_of_mismatches":9,"primer_efficiency":0.00069,"annealing_DeltaG":-5.607,"Position_3terminus":9,"All_mismatches":"24,23,21,19,17,15,14,13,9","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0,0,0","Primer_Sequence":"caaggagtctgttccgaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.58333,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":61.38,"Self_Dimer_DeltaG":-0.765,"Structure_deltaG_fw":-0.32,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"151_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH5","Template":"IGHV2-26","Group":"IGHV2-26","Number_of_mismatches":10,"primer_efficiency":0.00035,"annealing_DeltaG":-5.553,"Position_3terminus":1,"All_mismatches":"24,13,9,8,7,5,4,3,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"caaggagtctgttccgaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":5,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.58333,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":61.38,"Self_Dimer_DeltaG":-0.765,"Structure_deltaG_fw":-0.32,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":5,"_row":"152_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH5","Template":"IGHV2-5","Group":"IGHV2-5","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-5.553,"Position_3terminus":1,"All_mismatches":"24,13,9,7,5,4,3,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6","Primer_Sequence":"caaggagtctgttccgaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":5,"Experimental_Coverage":"Unamplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.58333,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":61.38,"Self_Dimer_DeltaG":-0.765,"Structure_deltaG_fw":-0.32,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":5,"_row":"153_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH5","Template":"IGHV2-70","Group":"IGHV2-70","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-2.036,"Position_3terminus":2,"All_mismatches":"24,22,21,20,18,16,9,8,6,4,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"caaggagtctgttccgaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.58333,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":61.38,"Self_Dimer_DeltaG":-0.765,"Structure_deltaG_fw":-0.32,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":3,"_row":"154_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH5","Template":"IGHV3-11","Group":"IGHV3-11","Number_of_mismatches":8,"primer_efficiency":0.0006,"annealing_DeltaG":-5.255,"Position_3terminus":9,"All_mismatches":"24,19,15,14,13,11,10,9","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0,0","Primer_Sequence":"caaggagtctgttccgaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.58333,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":61.38,"Self_Dimer_DeltaG":-0.765,"Structure_deltaG_fw":-0.32,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"155_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH5","Template":"IGHV3-13","Group":"IGHV3-13","Number_of_mismatches":7,"primer_efficiency":0.00074,"annealing_DeltaG":-5.39,"Position_3terminus":10,"All_mismatches":"24,19,15,14,13,11,10","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0","Primer_Sequence":"caaggagtctgttccgaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.58333,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":61.38,"Self_Dimer_DeltaG":-0.765,"Structure_deltaG_fw":-0.32,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"156_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH5","Template":"IGHV3-15","Group":"IGHV3-15","Number_of_mismatches":7,"primer_efficiency":0.00074,"annealing_DeltaG":-5.39,"Position_3terminus":10,"All_mismatches":"24,19,15,14,13,11,10","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0","Primer_Sequence":"caaggagtctgttccgaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.58333,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":61.38,"Self_Dimer_DeltaG":-0.765,"Structure_deltaG_fw":-0.32,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"157_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH5","Template":"IGHV3-20","Group":"IGHV3-20","Number_of_mismatches":7,"primer_efficiency":0.00074,"annealing_DeltaG":-5.39,"Position_3terminus":10,"All_mismatches":"24,19,15,14,13,11,10","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0","Primer_Sequence":"caaggagtctgttccgaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.58333,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":61.38,"Self_Dimer_DeltaG":-0.765,"Structure_deltaG_fw":-0.32,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"158_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH5","Template":"IGHV3-21","Group":"IGHV3-21","Number_of_mismatches":7,"primer_efficiency":0.00074,"annealing_DeltaG":-5.39,"Position_3terminus":10,"All_mismatches":"24,19,15,14,13,11,10","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0","Primer_Sequence":"caaggagtctgttccgaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.58333,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":61.38,"Self_Dimer_DeltaG":-0.765,"Structure_deltaG_fw":-0.32,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"159_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH5","Template":"IGHV3-23","Group":"IGHV3-23","Number_of_mismatches":7,"primer_efficiency":0.00074,"annealing_DeltaG":-5.39,"Position_3terminus":10,"All_mismatches":"24,19,15,14,13,11,10","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0","Primer_Sequence":"caaggagtctgttccgaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.58333,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":61.38,"Self_Dimer_DeltaG":-0.765,"Structure_deltaG_fw":-0.32,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"160_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH5","Template":"IGHV3-30","Group":"IGHV3-30","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-2.82,"Position_3terminus":1,"All_mismatches":"22,16,13,12,10,6,3,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6","Primer_Sequence":"caaggagtctgttccgaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.58333,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":61.38,"Self_Dimer_DeltaG":-0.765,"Structure_deltaG_fw":-0.32,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":4,"_row":"161_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH5","Template":"IGHV3-30-3","Group":"IGHV3-30-3","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-2.82,"Position_3terminus":1,"All_mismatches":"22,16,13,12,10,6,3,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6","Primer_Sequence":"caaggagtctgttccgaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.58333,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":61.38,"Self_Dimer_DeltaG":-0.765,"Structure_deltaG_fw":-0.32,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":4,"_row":"162_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH5","Template":"IGHV3-33","Group":"IGHV3-33","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-2.82,"Position_3terminus":1,"All_mismatches":"22,16,13,12,10,6,3,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6","Primer_Sequence":"caaggagtctgttccgaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.58333,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":61.38,"Self_Dimer_DeltaG":-0.765,"Structure_deltaG_fw":-0.32,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":4,"_row":"163_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH5","Template":"IGHV3-43","Group":"IGHV3-43","Number_of_mismatches":8,"primer_efficiency":0.00002,"annealing_DeltaG":-2.98,"Position_3terminus":7,"All_mismatches":"24,19,15,14,13,11,10,7","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0,0","Primer_Sequence":"caaggagtctgttccgaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Unamplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.58333,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":61.38,"Self_Dimer_DeltaG":-0.765,"Structure_deltaG_fw":-0.32,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"164_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH5","Template":"IGHV3-48","Group":"IGHV3-48","Number_of_mismatches":7,"primer_efficiency":0.00074,"annealing_DeltaG":-5.39,"Position_3terminus":10,"All_mismatches":"24,19,15,14,13,11,10","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0","Primer_Sequence":"caaggagtctgttccgaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.58333,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":61.38,"Self_Dimer_DeltaG":-0.765,"Structure_deltaG_fw":-0.32,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"165_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH5","Template":"IGHV3-49","Group":"IGHV3-49","Number_of_mismatches":7,"primer_efficiency":0.00074,"annealing_DeltaG":-5.39,"Position_3terminus":10,"All_mismatches":"24,19,15,14,13,11,10","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0","Primer_Sequence":"caaggagtctgttccgaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.58333,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":61.38,"Self_Dimer_DeltaG":-0.765,"Structure_deltaG_fw":-0.32,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"166_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH5","Template":"IGHV3-53","Group":"IGHV3-53","Number_of_mismatches":7,"primer_efficiency":0.00074,"annealing_DeltaG":-5.39,"Position_3terminus":10,"All_mismatches":"24,19,15,14,13,11,10","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0","Primer_Sequence":"caaggagtctgttccgaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.58333,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":61.38,"Self_Dimer_DeltaG":-0.765,"Structure_deltaG_fw":-0.32,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"167_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH5","Template":"IGHV3-64","Group":"IGHV3-64","Number_of_mismatches":7,"primer_efficiency":0.00074,"annealing_DeltaG":-5.39,"Position_3terminus":10,"All_mismatches":"24,19,15,14,13,11,10","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0","Primer_Sequence":"caaggagtctgttccgaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.58333,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":61.38,"Self_Dimer_DeltaG":-0.765,"Structure_deltaG_fw":-0.32,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"168_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH5","Template":"IGHV3-64D","Group":"IGHV3-64D","Number_of_mismatches":8,"primer_efficiency":0.00074,"annealing_DeltaG":-5.39,"Position_3terminus":10,"All_mismatches":"24,20,19,15,14,13,11,10","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0,0","Primer_Sequence":"caaggagtctgttccgaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.58333,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":61.38,"Self_Dimer_DeltaG":-0.765,"Structure_deltaG_fw":-0.32,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"169_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH5","Template":"IGHV3-66","Group":"IGHV3-66","Number_of_mismatches":7,"primer_efficiency":0.00074,"annealing_DeltaG":-5.39,"Position_3terminus":10,"All_mismatches":"24,19,15,14,13,11,10","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0","Primer_Sequence":"caaggagtctgttccgaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.58333,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":61.38,"Self_Dimer_DeltaG":-0.765,"Structure_deltaG_fw":-0.32,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"170_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH5","Template":"IGHV3-7","Group":"IGHV3-7","Number_of_mismatches":7,"primer_efficiency":0.00074,"annealing_DeltaG":-5.39,"Position_3terminus":10,"All_mismatches":"24,19,15,14,13,11,10","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0","Primer_Sequence":"caaggagtctgttccgaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.58333,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":61.38,"Self_Dimer_DeltaG":-0.765,"Structure_deltaG_fw":-0.32,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"171_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH5","Template":"IGHV3-72","Group":"IGHV3-72","Number_of_mismatches":6,"primer_efficiency":0.00074,"annealing_DeltaG":-5.39,"Position_3terminus":10,"All_mismatches":"19,15,14,13,11,10","Worst_Case_MismatchLocal":"0,0,0,0,0,0","Primer_Sequence":"caaggagtctgttccgaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.58333,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":61.38,"Self_Dimer_DeltaG":-0.765,"Structure_deltaG_fw":-0.32,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"172_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH5","Template":"IGHV3-73","Group":"IGHV3-73","Number_of_mismatches":7,"primer_efficiency":0.00074,"annealing_DeltaG":-5.39,"Position_3terminus":10,"All_mismatches":"24,19,15,14,13,11,10","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0","Primer_Sequence":"caaggagtctgttccgaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.58333,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":61.38,"Self_Dimer_DeltaG":-0.765,"Structure_deltaG_fw":-0.32,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"173_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH5","Template":"IGHV3-74","Group":"IGHV3-74","Number_of_mismatches":7,"primer_efficiency":0.00074,"annealing_DeltaG":-5.39,"Position_3terminus":10,"All_mismatches":"24,19,15,14,13,11,10","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0","Primer_Sequence":"caaggagtctgttccgaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.58333,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":61.38,"Self_Dimer_DeltaG":-0.765,"Structure_deltaG_fw":-0.32,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"174_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH5","Template":"IGHV3-9","Group":"IGHV3-9","Number_of_mismatches":8,"primer_efficiency":0.00002,"annealing_DeltaG":-2.98,"Position_3terminus":7,"All_mismatches":"24,19,15,14,13,11,10,7","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0,0","Primer_Sequence":"caaggagtctgttccgaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.58333,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":61.38,"Self_Dimer_DeltaG":-0.765,"Structure_deltaG_fw":-0.32,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"175_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH5","Template":"IGHV4-28","Group":"IGHV4-28","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-2.739,"Position_3terminus":4,"All_mismatches":"24,23,21,19,15,14,13,9,4","Worst_Case_MismatchLocal":"3,3,3,3,3,3,3,3,3","Primer_Sequence":"caaggagtctgttccgaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.58333,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":61.38,"Self_Dimer_DeltaG":-0.765,"Structure_deltaG_fw":-0.32,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":3,"Number_of_mismatches_hexamer":1,"_row":"176_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH5","Template":"IGHV4-30-2","Group":"IGHV4-30-2","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-3.867,"Position_3terminus":6,"All_mismatches":"24,23,21,19,15,14,13,9,6","Worst_Case_MismatchLocal":"1,1,1,1,1,1,1,1,1","Primer_Sequence":"caaggagtctgttccgaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.58333,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":61.38,"Self_Dimer_DeltaG":-0.765,"Structure_deltaG_fw":-0.32,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":1,"Number_of_mismatches_hexamer":1,"_row":"177_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH5","Template":"IGHV4-31","Group":"IGHV4-31","Number_of_mismatches":8,"primer_efficiency":0,"annealing_DeltaG":-5.607,"Position_3terminus":9,"All_mismatches":"24,23,21,19,15,14,13,9","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0,0","Primer_Sequence":"caaggagtctgttccgaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.58333,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":61.38,"Self_Dimer_DeltaG":-0.765,"Structure_deltaG_fw":-0.32,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"178_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH5","Template":"IGHV4-34","Group":"IGHV4-34","Number_of_mismatches":8,"primer_efficiency":0,"annealing_DeltaG":-5.607,"Position_3terminus":9,"All_mismatches":"24,23,21,19,15,14,13,9","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0,0","Primer_Sequence":"caaggagtctgttccgaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.58333,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":61.38,"Self_Dimer_DeltaG":-0.765,"Structure_deltaG_fw":-0.32,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"179_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH5","Template":"IGHV4-38-2","Group":"IGHV4-38-2","Number_of_mismatches":8,"primer_efficiency":0,"annealing_DeltaG":-5.607,"Position_3terminus":9,"All_mismatches":"24,23,21,19,15,14,13,9","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0,0","Primer_Sequence":"caaggagtctgttccgaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.58333,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":61.38,"Self_Dimer_DeltaG":-0.765,"Structure_deltaG_fw":-0.32,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"180_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH5","Template":"IGHV4-39","Group":"IGHV4-39","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-3.867,"Position_3terminus":6,"All_mismatches":"24,23,21,19,15,14,13,9,6","Worst_Case_MismatchLocal":"1,1,1,1,1,1,1,1,1","Primer_Sequence":"caaggagtctgttccgaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.58333,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":61.38,"Self_Dimer_DeltaG":-0.765,"Structure_deltaG_fw":-0.32,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":1,"Number_of_mismatches_hexamer":1,"_row":"181_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH5","Template":"IGHV4-4","Group":"IGHV4-4","Number_of_mismatches":8,"primer_efficiency":0,"annealing_DeltaG":-5.607,"Position_3terminus":9,"All_mismatches":"24,23,21,19,15,14,13,9","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0,0","Primer_Sequence":"caaggagtctgttccgaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.58333,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":61.38,"Self_Dimer_DeltaG":-0.765,"Structure_deltaG_fw":-0.32,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"182_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH5","Template":"IGHV4-59","Group":"IGHV4-59","Number_of_mismatches":8,"primer_efficiency":0,"annealing_DeltaG":-5.607,"Position_3terminus":9,"All_mismatches":"24,23,21,19,15,14,13,9","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0,0","Primer_Sequence":"caaggagtctgttccgaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.58333,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":61.38,"Self_Dimer_DeltaG":-0.765,"Structure_deltaG_fw":-0.32,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"183_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH5","Template":"IGHV4-61","Group":"IGHV4-61","Number_of_mismatches":8,"primer_efficiency":0,"annealing_DeltaG":-5.607,"Position_3terminus":9,"All_mismatches":"24,23,21,19,15,14,13,9","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0,0","Primer_Sequence":"caaggagtctgttccgaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.58333,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":61.38,"Self_Dimer_DeltaG":-0.765,"Structure_deltaG_fw":-0.32,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"184_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH5","Template":"IGHV5-10-1","Group":"IGHV5-10-1","Number_of_mismatches":2,"primer_efficiency":0.77839,"annealing_DeltaG":-11.115,"Position_3terminus":7,"All_mismatches":"12,7","Worst_Case_MismatchLocal":"0,0","Primer_Sequence":"caaggagtctgttccgaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.58333,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":61.38,"Self_Dimer_DeltaG":-0.765,"Structure_deltaG_fw":-0.32,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"185_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH5","Template":"IGHV5-51","Group":"IGHV5-51","Number_of_mismatches":1,"primer_efficiency":0.99623,"annealing_DeltaG":-13.949,"Position_3terminus":12,"All_mismatches":"12","Worst_Case_MismatchLocal":"0","Primer_Sequence":"caaggagtctgttccgaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.58333,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":61.38,"Self_Dimer_DeltaG":-0.765,"Structure_deltaG_fw":-0.32,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"186_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH5","Template":"IGHV6-1","Group":"IGHV6-1","Number_of_mismatches":10,"primer_efficiency":2.3429e-06,"annealing_DeltaG":-2.387,"Position_3terminus":4,"All_mismatches":"24,23,22,19,15,14,13,10,9,4","Worst_Case_MismatchLocal":"3,3,3,3,3,3,3,3,3,3","Primer_Sequence":"caaggagtctgttccgaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Unamplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.58333,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":61.38,"Self_Dimer_DeltaG":-0.765,"Structure_deltaG_fw":-0.32,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":3,"Number_of_mismatches_hexamer":1,"_row":"187_Tiller2008_1st"}
{"Primer":"Tiller_5'LVH5","Template":"IGHV7-4-1","Group":"IGHV7-4-1","Number_of_mismatches":8,"primer_efficiency":0.00053,"annealing_DeltaG":-5.607,"Position_3terminus":9,"All_mismatches":"24,23,19,17,15,14,13,9","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0,0","Primer_Sequence":"caaggagtctgttccgaggtgcag","Terminal_Dinucleotide":"ag","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.58333,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":61.38,"Self_Dimer_DeltaG":-0.765,"Structure_deltaG_fw":-0.32,"primer_coverage":47,"primer_specificity":0.5,"Run":"Tiller2008_1st","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"188_Tiller2008_1st"}
{"Primer":"Opt5_1","Template":"IGHV1-18","Group":"IGHV1-18","Number_of_mismatches":12,"primer_efficiency":0,"annealing_DeltaG":-2.354,"Position_3terminus":1,"All_mismatches":"21,17,16,13,12,11,10,6,4,3,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"cacctgtggttcttcctcctcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":5,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.05,"Self_Dimer_DeltaG":-0.81,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":5,"_row":"1_openPrimeR2017"}
{"Primer":"Opt5_1","Template":"IGHV1-2","Group":"IGHV1-2","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-2.026,"Position_3terminus":2,"All_mismatches":"22,19,16,13,12,10,7,6,3,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"cacctgtggttcttcctcctcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.05,"Self_Dimer_DeltaG":-0.81,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":3,"_row":"2_openPrimeR2017"}
{"Primer":"Opt5_1","Template":"IGHV1-24","Group":"IGHV1-24","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-1.243,"Position_3terminus":2,"All_mismatches":"22,19,16,13,12,10,7,6,5,3,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"cacctgtggttcttcctcctcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.05,"Self_Dimer_DeltaG":-0.81,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":4,"_row":"3_openPrimeR2017"}
{"Primer":"Opt5_1","Template":"IGHV1-3","Group":"IGHV1-3","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-1.481,"Position_3terminus":2,"All_mismatches":"22,19,16,13,12,10,9,7,3,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"cacctgtggttcttcctcctcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.05,"Self_Dimer_DeltaG":-0.81,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":2,"_row":"4_openPrimeR2017"}
{"Primer":"Opt5_1","Template":"IGHV1-45","Group":"IGHV1-45","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-2.403,"Position_3terminus":2,"All_mismatches":"22,21,16,15,14,13,12,6,5,4,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"cacctgtggttcttcctcctcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.05,"Self_Dimer_DeltaG":-0.81,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":4,"_row":"5_openPrimeR2017"}
{"Primer":"Opt5_1","Template":"IGHV1-46","Group":"IGHV1-46","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-2.217,"Position_3terminus":2,"All_mismatches":"22,21,19,18,16,13,7,6,4,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"cacctgtggttcttcctcctcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.05,"Self_Dimer_DeltaG":-0.81,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":3,"_row":"6_openPrimeR2017"}
{"Primer":"Opt5_1","Template":"IGHV1-58","Group":"IGHV1-58","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-2.093,"Position_3terminus":1,"All_mismatches":"22,21,20,19,17,13,12,11,10,4,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"cacctgtggttcttcctcctcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.05,"Self_Dimer_DeltaG":-0.81,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":2,"_row":"7_openPrimeR2017"}
{"Primer":"Opt5_1","Template":"IGHV1-69","Group":"IGHV1-69","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-3.171,"Position_3terminus":7,"All_mismatches":"19,17,16,15,14,13,12,10,9,8,7","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0,0,0,0,0","Primer_Sequence":"cacctgtggttcttcctcctcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.05,"Self_Dimer_DeltaG":-0.81,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"8_openPrimeR2017"}
{"Primer":"Opt5_1","Template":"IGHV1-69-2","Group":"IGHV1-69-2","Number_of_mismatches":10,"primer_efficiency":0.00002,"annealing_DeltaG":-4.647,"Position_3terminus":1,"All_mismatches":"22,21,19,18,17,13,12,11,10,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"cacctgtggttcttcctcctcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.05,"Self_Dimer_DeltaG":-0.81,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":1,"_row":"9_openPrimeR2017"}
{"Primer":"Opt5_1","Template":"IGHV1-8","Group":"IGHV1-8","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-3.492,"Position_3terminus":1,"All_mismatches":"22,21,14,13,12,11,10,9,8,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"cacctgtggttcttcctcctcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.05,"Self_Dimer_DeltaG":-0.81,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":1,"_row":"10_openPrimeR2017"}
{"Primer":"Opt5_1","Template":"IGHV2-26","Group":"IGHV2-26","Number_of_mismatches":10,"primer_efficiency":2.96506e-06,"annealing_DeltaG":-2.403,"Position_3terminus":2,"All_mismatches":"22,21,20,17,14,12,10,9,8,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"cacctgtggttcttcctcctcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.05,"Self_Dimer_DeltaG":-0.81,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":1,"_row":"11_openPrimeR2017"}
{"Primer":"Opt5_1","Template":"IGHV2-5","Group":"IGHV2-5","Number_of_mismatches":10,"primer_efficiency":2.99247e-06,"annealing_DeltaG":-2.409,"Position_3terminus":2,"All_mismatches":"22,21,20,17,14,12,10,9,8,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"cacctgtggttcttcctcctcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.05,"Self_Dimer_DeltaG":-0.81,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":1,"_row":"12_openPrimeR2017"}
{"Primer":"Opt5_1","Template":"IGHV2-70","Group":"IGHV2-70","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-2.403,"Position_3terminus":2,"All_mismatches":"22,19,16,13,12,11,9,8,7,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"cacctgtggttcttcctcctcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.05,"Self_Dimer_DeltaG":-0.81,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":1,"_row":"13_openPrimeR2017"}
{"Primer":"Opt5_1","Template":"IGHV3-11","Group":"IGHV3-11","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-2.052,"Position_3terminus":1,"All_mismatches":"21,20,19,18,17,13,11,5,4,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"cacctgtggttcttcctcctcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.05,"Self_Dimer_DeltaG":-0.81,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":4,"_row":"14_openPrimeR2017"}
{"Primer":"Opt5_1","Template":"IGHV3-13","Group":"IGHV3-13","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-4.189,"Position_3terminus":2,"All_mismatches":"21,14,13,12,10,7,6,5,4,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"cacctgtggttcttcctcctcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Amplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.05,"Self_Dimer_DeltaG":-0.81,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":4,"_row":"15_openPrimeR2017"}
{"Primer":"Opt5_1","Template":"IGHV3-15","Group":"IGHV3-15","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-4.189,"Position_3terminus":2,"All_mismatches":"21,14,13,12,10,7,6,5,4,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"cacctgtggttcttcctcctcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Amplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.05,"Self_Dimer_DeltaG":-0.81,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":4,"_row":"16_openPrimeR2017"}
{"Primer":"Opt5_1","Template":"IGHV3-20","Group":"IGHV3-20","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-4.189,"Position_3terminus":2,"All_mismatches":"21,14,13,12,10,7,6,5,4,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"cacctgtggttcttcctcctcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.05,"Self_Dimer_DeltaG":-0.81,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":4,"_row":"17_openPrimeR2017"}
{"Primer":"Opt5_1","Template":"IGHV3-21","Group":"IGHV3-21","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-2.052,"Position_3terminus":1,"All_mismatches":"21,18,17,13,11,5,4,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6","Primer_Sequence":"cacctgtggttcttcctcctcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.05,"Self_Dimer_DeltaG":-0.81,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":4,"_row":"18_openPrimeR2017"}
{"Primer":"Opt5_1","Template":"IGHV3-23","Group":"IGHV3-23","Number_of_mismatches":12,"primer_efficiency":0,"annealing_DeltaG":-1.62,"Position_3terminus":1,"All_mismatches":"20,18,17,13,12,11,8,6,5,4,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"cacctgtggttcttcctcctcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":5,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.05,"Self_Dimer_DeltaG":-0.81,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":5,"_row":"19_openPrimeR2017"}
{"Primer":"Opt5_1","Template":"IGHV3-30","Group":"IGHV3-30","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-2.093,"Position_3terminus":1,"All_mismatches":"21,20,19,18,17,13,11,4,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"cacctgtggttcttcctcctcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.05,"Self_Dimer_DeltaG":-0.81,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"20_openPrimeR2017"}
{"Primer":"Opt5_1","Template":"IGHV3-30-3","Group":"IGHV3-30-3","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-2.093,"Position_3terminus":1,"All_mismatches":"21,20,19,18,17,13,11,4,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"cacctgtggttcttcctcctcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.05,"Self_Dimer_DeltaG":-0.81,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"21_openPrimeR2017"}
{"Primer":"Opt5_1","Template":"IGHV3-33","Group":"IGHV3-33","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-2.093,"Position_3terminus":1,"All_mismatches":"21,20,19,18,17,13,11,4,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"cacctgtggttcttcctcctcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.05,"Self_Dimer_DeltaG":-0.81,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"22_openPrimeR2017"}
{"Primer":"Opt5_1","Template":"IGHV3-43","Group":"IGHV3-43","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-2.052,"Position_3terminus":1,"All_mismatches":"21,20,19,18,17,13,11,5,4,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"cacctgtggttcttcctcctcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.05,"Self_Dimer_DeltaG":-0.81,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":4,"_row":"23_openPrimeR2017"}
{"Primer":"Opt5_1","Template":"IGHV3-48","Group":"IGHV3-48","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-2.052,"Position_3terminus":1,"All_mismatches":"22,21,20,14,12,11,10,8,7,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"cacctgtggttcttcctcctcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.05,"Self_Dimer_DeltaG":-0.81,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":2,"_row":"24_openPrimeR2017"}
{"Primer":"Opt5_1","Template":"IGHV3-49","Group":"IGHV3-49","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-2.052,"Position_3terminus":1,"All_mismatches":"21,20,19,18,17,13,11,5,4,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"cacctgtggttcttcctcctcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.05,"Self_Dimer_DeltaG":-0.81,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":4,"_row":"25_openPrimeR2017"}
{"Primer":"Opt5_1","Template":"IGHV3-53","Group":"IGHV3-53","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-2.052,"Position_3terminus":1,"All_mismatches":"21,20,19,18,17,13,11,5,4,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"cacctgtggttcttcctcctcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.05,"Self_Dimer_DeltaG":-0.81,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":4,"_row":"26_openPrimeR2017"}
{"Primer":"Opt5_1","Template":"IGHV3-64","Group":"IGHV3-64","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-2.052,"Position_3terminus":1,"All_mismatches":"21,20,19,18,17,13,11,5,4,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"cacctgtggttcttcctcctcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.05,"Self_Dimer_DeltaG":-0.81,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":4,"_row":"27_openPrimeR2017"}
{"Primer":"Opt5_1","Template":"IGHV3-64D","Group":"IGHV3-64D","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-0.321,"Position_3terminus":1,"All_mismatches":"22,19,16,13,12,11,10,7,4,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"cacctgtggttcttcctcctcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.05,"Self_Dimer_DeltaG":-0.81,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"28_openPrimeR2017"}
{"Primer":"Opt5_1","Template":"IGHV3-66","Group":"IGHV3-66","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-2.052,"Position_3terminus":1,"All_mismatches":"21,20,19,18,17,13,11,5,4,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"cacctgtggttcttcctcctcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.05,"Self_Dimer_DeltaG":-0.81,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":4,"_row":"29_openPrimeR2017"}
{"Primer":"Opt5_1","Template":"IGHV3-7","Group":"IGHV3-7","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-2.052,"Position_3terminus":1,"All_mismatches":"21,20,19,18,17,13,11,5,4,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"cacctgtggttcttcctcctcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.05,"Self_Dimer_DeltaG":-0.81,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":4,"_row":"30_openPrimeR2017"}
{"Primer":"Opt5_1","Template":"IGHV3-72","Group":"IGHV3-72","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-4.189,"Position_3terminus":2,"All_mismatches":"21,14,13,12,10,7,6,5,4,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"cacctgtggttcttcctcctcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.05,"Self_Dimer_DeltaG":-0.81,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":4,"_row":"31_openPrimeR2017"}
{"Primer":"Opt5_1","Template":"IGHV3-73","Group":"IGHV3-73","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-4.189,"Position_3terminus":2,"All_mismatches":"21,14,13,12,10,7,6,5,4,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"cacctgtggttcttcctcctcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.05,"Self_Dimer_DeltaG":-0.81,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":4,"_row":"32_openPrimeR2017"}
{"Primer":"Opt5_1","Template":"IGHV3-74","Group":"IGHV3-74","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-1.464,"Position_3terminus":1,"All_mismatches":"21,19,18,17,14,9,7,5,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"cacctgtggttcttcctcctcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.05,"Self_Dimer_DeltaG":-0.81,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"33_openPrimeR2017"}
{"Primer":"Opt5_1","Template":"IGHV3-9","Group":"IGHV3-9","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-2.052,"Position_3terminus":1,"All_mismatches":"21,20,19,18,17,13,11,5,4,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"cacctgtggttcttcctcctcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.05,"Self_Dimer_DeltaG":-0.81,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":4,"_row":"34_openPrimeR2017"}
{"Primer":"Opt5_1","Template":"IGHV4-28","Group":"IGHV4-28","Number_of_mismatches":1,"primer_efficiency":0.37124,"annealing_DeltaG":-14.446,"Position_3terminus":2,"All_mismatches":"2","Worst_Case_MismatchLocal":"5","Primer_Sequence":"cacctgtggttcttcctcctcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Amplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.05,"Self_Dimer_DeltaG":-0.81,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":1,"_row":"35_openPrimeR2017"}
{"Primer":"Opt5_1","Template":"IGHV4-30-2","Group":"IGHV4-30-2","Number_of_mismatches":1,"primer_efficiency":0.37124,"annealing_DeltaG":-14.446,"Position_3terminus":2,"All_mismatches":"2","Worst_Case_MismatchLocal":"5","Primer_Sequence":"cacctgtggttcttcctcctcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Amplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.05,"Self_Dimer_DeltaG":-0.81,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":1,"_row":"36_openPrimeR2017"}
{"Primer":"Opt5_1","Template":"IGHV4-31","Group":"IGHV4-31","Number_of_mismatches":1,"primer_efficiency":0.37124,"annealing_DeltaG":-14.446,"Position_3terminus":2,"All_mismatches":"2","Worst_Case_MismatchLocal":"5","Primer_Sequence":"cacctgtggttcttcctcctcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Amplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.05,"Self_Dimer_DeltaG":-0.81,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":1,"_row":"37_openPrimeR2017"}
{"Primer":"Opt5_1","Template":"IGHV4-34","Group":"IGHV4-34","Number_of_mismatches":0,"primer_efficiency":0.99966,"annealing_DeltaG":-15.609,"All_mismatches":"NA","Worst_Case_MismatchLocal":"0","Primer_Sequence":"cacctgtggttcttcctcctcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.05,"Self_Dimer_DeltaG":-0.81,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"38_openPrimeR2017"}
{"Primer":"Opt5_1","Template":"IGHV4-38-2","Group":"IGHV4-38-2","Number_of_mismatches":7,"primer_efficiency":0.03905,"annealing_DeltaG":-9.013,"Position_3terminus":1,"All_mismatches":"11,9,7,6,4,3,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6","Primer_Sequence":"cacctgtggttcttcctcctcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Amplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.05,"Self_Dimer_DeltaG":-0.81,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":4,"_row":"39_openPrimeR2017"}
{"Primer":"Opt5_1","Template":"IGHV4-39","Group":"IGHV4-39","Number_of_mismatches":1,"primer_efficiency":0.37124,"annealing_DeltaG":-14.446,"Position_3terminus":2,"All_mismatches":"2","Worst_Case_MismatchLocal":"5","Primer_Sequence":"cacctgtggttcttcctcctcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Amplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.05,"Self_Dimer_DeltaG":-0.81,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":1,"_row":"40_openPrimeR2017"}
{"Primer":"Opt5_1","Template":"IGHV4-4","Group":"IGHV4-4","Number_of_mismatches":1,"primer_efficiency":0.37124,"annealing_DeltaG":-14.446,"Position_3terminus":2,"All_mismatches":"2","Worst_Case_MismatchLocal":"5","Primer_Sequence":"cacctgtggttcttcctcctcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Amplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.05,"Self_Dimer_DeltaG":-0.81,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":1,"_row":"41_openPrimeR2017"}
{"Primer":"Opt5_1","Template":"IGHV4-59","Group":"IGHV4-59","Number_of_mismatches":2,"primer_efficiency":0.30507,"annealing_DeltaG":-9.863,"Position_3terminus":5,"All_mismatches":"20,5","Worst_Case_MismatchLocal":"2,2","Primer_Sequence":"cacctgtggttcttcctcctcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Amplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.05,"Self_Dimer_DeltaG":-0.81,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":2,"Number_of_mismatches_hexamer":1,"_row":"42_openPrimeR2017"}
{"Primer":"Opt5_1","Template":"IGHV4-61","Group":"IGHV4-61","Number_of_mismatches":0,"primer_efficiency":0.99966,"annealing_DeltaG":-15.609,"All_mismatches":"NA","Worst_Case_MismatchLocal":"0","Primer_Sequence":"cacctgtggttcttcctcctcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.05,"Self_Dimer_DeltaG":-0.81,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"43_openPrimeR2017"}
{"Primer":"Opt5_1","Template":"IGHV5-10-1","Group":"IGHV5-10-1","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-1.304,"Position_3terminus":2,"All_mismatches":"20,19,18,17,16,13,10,9,6,3,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"cacctgtggttcttcctcctcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Amplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.05,"Self_Dimer_DeltaG":-0.81,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":3,"_row":"44_openPrimeR2017"}
{"Primer":"Opt5_1","Template":"IGHV5-51","Group":"IGHV5-51","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-1.304,"Position_3terminus":2,"All_mismatches":"20,18,17,16,13,10,9,6,3,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"cacctgtggttcttcctcctcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.05,"Self_Dimer_DeltaG":-0.81,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":3,"_row":"45_openPrimeR2017"}
{"Primer":"Opt5_1","Template":"IGHV6-1","Group":"IGHV6-1","Number_of_mismatches":10,"primer_efficiency":8.19293e-06,"annealing_DeltaG":-3.113,"Position_3terminus":1,"All_mismatches":"22,21,20,19,15,14,13,9,7,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"cacctgtggttcttcctcctcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.05,"Self_Dimer_DeltaG":-0.81,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":1,"_row":"46_openPrimeR2017"}
{"Primer":"Opt5_1","Template":"IGHV7-4-1","Group":"IGHV7-4-1","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-2.026,"Position_3terminus":2,"All_mismatches":"22,19,16,13,10,9,6,5,3,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"cacctgtggttcttcctcctcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.05,"Self_Dimer_DeltaG":-0.81,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":4,"_row":"47_openPrimeR2017"}
{"Primer":"Opt5_10","Template":"IGHV1-18","Group":"IGHV1-18","Number_of_mismatches":4,"primer_efficiency":0,"annealing_DeltaG":-12.218,"Position_3terminus":1,"All_mismatches":"7,6,3,1","Worst_Case_MismatchLocal":"6,6,6,6","Primer_Sequence":"atggactggacctggagggtcttc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.58333,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":61.95,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":-3.56,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"48_openPrimeR2017"}
{"Primer":"Opt5_10","Template":"IGHV1-2","Group":"IGHV1-2","Number_of_mismatches":2,"primer_efficiency":0,"annealing_DeltaG":-13.564,"Position_3terminus":3,"All_mismatches":"6,3","Worst_Case_MismatchLocal":"4,4","Primer_Sequence":"atggactggacctggagggtcttc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.58333,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":61.95,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":-3.56,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":4,"Number_of_mismatches_hexamer":2,"_row":"49_openPrimeR2017"}
{"Primer":"Opt5_10","Template":"IGHV1-24","Group":"IGHV1-24","Number_of_mismatches":3,"primer_efficiency":0,"annealing_DeltaG":-11.607,"Position_3terminus":3,"All_mismatches":"16,6,3","Worst_Case_MismatchLocal":"4,4,4","Primer_Sequence":"atggactggacctggagggtcttc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.58333,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":61.95,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":-3.56,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":4,"Number_of_mismatches_hexamer":2,"_row":"50_openPrimeR2017"}
{"Primer":"Opt5_10","Template":"IGHV1-3","Group":"IGHV1-3","Number_of_mismatches":2,"primer_efficiency":0,"annealing_DeltaG":-13.564,"Position_3terminus":3,"All_mismatches":"6,3","Worst_Case_MismatchLocal":"4,4","Primer_Sequence":"atggactggacctggagggtcttc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.58333,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":61.95,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":-3.56,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":4,"Number_of_mismatches_hexamer":2,"_row":"51_openPrimeR2017"}
{"Primer":"Opt5_10","Template":"IGHV1-45","Group":"IGHV1-45","Number_of_mismatches":3,"primer_efficiency":0,"annealing_DeltaG":-12.133,"Position_3terminus":3,"All_mismatches":"7,6,3","Worst_Case_MismatchLocal":"4,4,4","Primer_Sequence":"atggactggacctggagggtcttc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.58333,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":61.95,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":-3.56,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":4,"Number_of_mismatches_hexamer":2,"_row":"52_openPrimeR2017"}
{"Primer":"Opt5_10","Template":"IGHV1-46","Group":"IGHV1-46","Number_of_mismatches":0,"primer_efficiency":0.99995,"annealing_DeltaG":-17.787,"All_mismatches":"NA","Worst_Case_MismatchLocal":"0","Primer_Sequence":"atggactggacctggagggtcttc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.58333,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":61.95,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":-3.56,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"53_openPrimeR2017"}
{"Primer":"Opt5_10","Template":"IGHV1-58","Group":"IGHV1-58","Number_of_mismatches":4,"primer_efficiency":0,"annealing_DeltaG":-7.69,"Position_3terminus":3,"All_mismatches":"14,13,6,3","Worst_Case_MismatchLocal":"4,4,4,4","Primer_Sequence":"atggactggacctggagggtcttc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.58333,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":61.95,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":-3.56,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":4,"Number_of_mismatches_hexamer":2,"_row":"54_openPrimeR2017"}
{"Primer":"Opt5_10","Template":"IGHV1-69","Group":"IGHV1-69","Number_of_mismatches":2,"primer_efficiency":0,"annealing_DeltaG":-14.072,"Position_3terminus":3,"All_mismatches":"6,3","Worst_Case_MismatchLocal":"4,4","Primer_Sequence":"atggactggacctggagggtcttc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.58333,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":61.95,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":-3.56,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":4,"Number_of_mismatches_hexamer":2,"_row":"55_openPrimeR2017"}
{"Primer":"Opt5_10","Template":"IGHV1-69-2","Group":"IGHV1-69-2","Number_of_mismatches":3,"primer_efficiency":0,"annealing_DeltaG":-11.607,"Position_3terminus":3,"All_mismatches":"16,6,3","Worst_Case_MismatchLocal":"4,4,4","Primer_Sequence":"atggactggacctggagggtcttc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.58333,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":61.95,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":-3.56,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":4,"Number_of_mismatches_hexamer":2,"_row":"56_openPrimeR2017"}
{"Primer":"Opt5_10","Template":"IGHV1-8","Group":"IGHV1-8","Number_of_mismatches":2,"primer_efficiency":0,"annealing_DeltaG":-13.564,"Position_3terminus":3,"All_mismatches":"6,3","Worst_Case_MismatchLocal":"4,4","Primer_Sequence":"atggactggacctggagggtcttc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.58333,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":61.95,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":-3.56,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":4,"Number_of_mismatches_hexamer":2,"_row":"57_openPrimeR2017"}
{"Primer":"Opt5_10","Template":"IGHV2-26","Group":"IGHV2-26","Number_of_mismatches":12,"primer_efficiency":0,"annealing_DeltaG":-1.695,"Position_3terminus":1,"All_mismatches":"23,21,19,18,16,13,9,7,4,3,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"atggactggacctggagggtcttc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.58333,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":61.95,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":-3.56,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":4,"_row":"58_openPrimeR2017"}
{"Primer":"Opt5_10","Template":"IGHV2-5","Group":"IGHV2-5","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-3.802,"Position_3terminus":4,"All_mismatches":"24,21,20,16,11,10,9,8,7,5,4","Worst_Case_MismatchLocal":"3,3,3,3,3,3,3,3,3,3,3","Primer_Sequence":"atggactggacctggagggtcttc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.58333,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":61.95,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":-3.56,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":3,"Number_of_mismatches_hexamer":2,"_row":"59_openPrimeR2017"}
{"Primer":"Opt5_10","Template":"IGHV2-70","Group":"IGHV2-70","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-3.802,"Position_3terminus":4,"All_mismatches":"24,21,20,16,11,10,9,8,7,5,4","Worst_Case_MismatchLocal":"3,3,3,3,3,3,3,3,3,3,3","Primer_Sequence":"atggactggacctggagggtcttc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.58333,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":61.95,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":-3.56,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":3,"Number_of_mismatches_hexamer":2,"_row":"60_openPrimeR2017"}
{"Primer":"Opt5_10","Template":"IGHV3-11","Group":"IGHV3-11","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-2.72,"Position_3terminus":2,"All_mismatches":"24,23,17,16,15,13,7,6,4,3,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"atggactggacctggagggtcttc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.58333,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":61.95,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":-3.56,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":4,"_row":"61_openPrimeR2017"}
{"Primer":"Opt5_10","Template":"IGHV3-13","Group":"IGHV3-13","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-2.738,"Position_3terminus":1,"All_mismatches":"24,21,20,17,16,15,12,8,5,4,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"atggactggacctggagggtcttc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.58333,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":61.95,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":-3.56,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"62_openPrimeR2017"}
{"Primer":"Opt5_10","Template":"IGHV3-15","Group":"IGHV3-15","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-2.72,"Position_3terminus":2,"All_mismatches":"24,23,17,16,15,13,7,6,4,3,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"atggactggacctggagggtcttc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.58333,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":61.95,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":-3.56,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":4,"_row":"63_openPrimeR2017"}
{"Primer":"Opt5_10","Template":"IGHV3-20","Group":"IGHV3-20","Number_of_mismatches":12,"primer_efficiency":0,"annealing_DeltaG":-2.185,"Position_3terminus":2,"All_mismatches":"23,22,21,19,17,15,13,7,6,4,3,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"atggactggacctggagggtcttc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.58333,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":61.95,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":-3.56,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":4,"_row":"64_openPrimeR2017"}
{"Primer":"Opt5_10","Template":"IGHV3-21","Group":"IGHV3-21","Number_of_mismatches":12,"primer_efficiency":0,"annealing_DeltaG":-2.672,"Position_3terminus":1,"All_mismatches":"23,21,19,18,16,13,9,6,5,4,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"atggactggacctggagggtcttc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":5,"Experimental_Coverage":"Unamplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.58333,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":61.95,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":-3.56,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":5,"_row":"65_openPrimeR2017"}
{"Primer":"Opt5_10","Template":"IGHV3-23","Group":"IGHV3-23","Number_of_mismatches":12,"primer_efficiency":0,"annealing_DeltaG":-3.072,"Position_3terminus":2,"All_mismatches":"24,23,16,13,12,11,7,6,5,4,3,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"atggactggacctggagggtcttc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":5,"Experimental_Coverage":"Unamplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.58333,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":61.95,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":-3.56,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":5,"_row":"66_openPrimeR2017"}
{"Primer":"Opt5_10","Template":"IGHV3-30","Group":"IGHV3-30","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-1.104,"Position_3terminus":1,"All_mismatches":"24,23,21,17,14,13,11,9,8,4,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"atggactggacctggagggtcttc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.58333,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":61.95,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":-3.56,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":2,"_row":"67_openPrimeR2017"}
{"Primer":"Opt5_10","Template":"IGHV3-30-3","Group":"IGHV3-30-3","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-3.486,"Position_3terminus":1,"All_mismatches":"24,23,21,14,13,11,9,8,4,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"atggactggacctggagggtcttc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.58333,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":61.95,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":-3.56,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":2,"_row":"68_openPrimeR2017"}
{"Primer":"Opt5_10","Template":"IGHV3-33","Group":"IGHV3-33","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-1.54,"Position_3terminus":1,"All_mismatches":"24,23,21,17,14,13,11,9,8,4,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"atggactggacctggagggtcttc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.58333,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":61.95,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":-3.56,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":2,"_row":"69_openPrimeR2017"}
{"Primer":"Opt5_10","Template":"IGHV3-43","Group":"IGHV3-43","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-2.778,"Position_3terminus":1,"All_mismatches":"23,21,19,18,16,13,9,8,6,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"atggactggacctggagggtcttc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.58333,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":61.95,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":-3.56,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"70_openPrimeR2017"}
{"Primer":"Opt5_10","Template":"IGHV3-48","Group":"IGHV3-48","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-1.695,"Position_3terminus":3,"All_mismatches":"23,21,19,18,16,13,9,7,5,4,3","Worst_Case_MismatchLocal":"4,4,4,4,4,4,4,4,4,4,4","Primer_Sequence":"atggactggacctggagggtcttc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.58333,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":61.95,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":-3.56,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":4,"Number_of_mismatches_hexamer":3,"_row":"71_openPrimeR2017"}
{"Primer":"Opt5_10","Template":"IGHV3-49","Group":"IGHV3-49","Number_of_mismatches":12,"primer_efficiency":0,"annealing_DeltaG":-1.695,"Position_3terminus":1,"All_mismatches":"23,21,19,18,16,13,9,7,5,4,3,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"atggactggacctggagggtcttc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.58333,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":61.95,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":-3.56,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":4,"_row":"72_openPrimeR2017"}
{"Primer":"Opt5_10","Template":"IGHV3-53","Group":"IGHV3-53","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-4.799,"Position_3terminus":1,"All_mismatches":"24,23,15,14,13,11,10,9,8,4,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"atggactggacctggagggtcttc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.58333,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":61.95,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":-3.56,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":2,"_row":"73_openPrimeR2017"}
{"Primer":"Opt5_10","Template":"IGHV3-64","Group":"IGHV3-64","Number_of_mismatches":12,"primer_efficiency":0,"annealing_DeltaG":-1.695,"Position_3terminus":1,"All_mismatches":"23,21,19,18,16,13,9,5,4,3,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"atggactggacctggagggtcttc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":5,"Experimental_Coverage":"Unamplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.58333,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":61.95,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":-3.56,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":5,"_row":"74_openPrimeR2017"}
{"Primer":"Opt5_10","Template":"IGHV3-64D","Group":"IGHV3-64D","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-1.754,"Position_3terminus":1,"All_mismatches":"23,21,19,18,16,13,9,7,5,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"atggactggacctggagggtcttc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.58333,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":61.95,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":-3.56,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"75_openPrimeR2017"}
{"Primer":"Opt5_10","Template":"IGHV3-66","Group":"IGHV3-66","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-2.72,"Position_3terminus":2,"All_mismatches":"24,23,17,15,13,7,6,4,3,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"atggactggacctggagggtcttc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.58333,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":61.95,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":-3.56,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":4,"_row":"76_openPrimeR2017"}
{"Primer":"Opt5_10","Template":"IGHV3-7","Group":"IGHV3-7","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-3.846,"Position_3terminus":4,"All_mismatches":"24,23,21,20,13,12,11,10,6,5,4","Worst_Case_MismatchLocal":"3,3,3,3,3,3,3,3,3,3,3","Primer_Sequence":"atggactggacctggagggtcttc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.58333,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":61.95,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":-3.56,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":3,"Number_of_mismatches_hexamer":3,"_row":"77_openPrimeR2017"}
{"Primer":"Opt5_10","Template":"IGHV3-72","Group":"IGHV3-72","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-2.72,"Position_3terminus":2,"All_mismatches":"24,23,17,16,15,13,7,6,4,3,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"atggactggacctggagggtcttc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.58333,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":61.95,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":-3.56,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":4,"_row":"78_openPrimeR2017"}
{"Primer":"Opt5_10","Template":"IGHV3-73","Group":"IGHV3-73","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-3.725,"Position_3terminus":2,"All_mismatches":"21,15,14,12,10,9,8,5,4,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"atggactggacctggagggtcttc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.58333,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":61.95,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":-3.56,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":3,"_row":"79_openPrimeR2017"}
{"Primer":"Opt5_10","Template":"IGHV3-74","Group":"IGHV3-74","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-1.695,"Position_3terminus":2,"All_mismatches":"23,21,19,18,16,13,9,6,5,3,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"atggactggacctggagggtcttc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.58333,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":61.95,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":-3.56,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":4,"_row":"80_openPrimeR2017"}
{"Primer":"Opt5_10","Template":"IGHV3-9","Group":"IGHV3-9","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-1.695,"Position_3terminus":1,"All_mismatches":"23,21,19,18,16,13,9,7,5,3,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"atggactggacctggagggtcttc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.58333,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":61.95,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":-3.56,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"81_openPrimeR2017"}
{"Primer":"Opt5_10","Template":"IGHV4-28","Group":"IGHV4-28","Number_of_mismatches":12,"primer_efficiency":0,"annealing_DeltaG":-3.127,"Position_3terminus":1,"All_mismatches":"24,20,19,18,17,16,15,12,10,9,3,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"atggactggacctggagggtcttc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.58333,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":61.95,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":-3.56,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":2,"_row":"82_openPrimeR2017"}
{"Primer":"Opt5_10","Template":"IGHV4-30-2","Group":"IGHV4-30-2","Number_of_mismatches":12,"primer_efficiency":0,"annealing_DeltaG":-2.608,"Position_3terminus":1,"All_mismatches":"23,21,19,18,16,13,9,7,6,4,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"atggactggacctggagggtcttc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.58333,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":61.95,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":-3.56,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":4,"_row":"83_openPrimeR2017"}
{"Primer":"Opt5_10","Template":"IGHV4-31","Group":"IGHV4-31","Number_of_mismatches":12,"primer_efficiency":0,"annealing_DeltaG":-2.779,"Position_3terminus":1,"All_mismatches":"23,21,19,18,16,13,9,5,4,3,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"atggactggacctggagggtcttc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":5,"Experimental_Coverage":"Unamplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.58333,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":61.95,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":-3.56,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":5,"_row":"84_openPrimeR2017"}
{"Primer":"Opt5_10","Template":"IGHV4-34","Group":"IGHV4-34","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-1.695,"Position_3terminus":1,"All_mismatches":"23,21,19,18,16,13,9,7,4,3,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"atggactggacctggagggtcttc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.58333,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":61.95,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":-3.56,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"85_openPrimeR2017"}
{"Primer":"Opt5_10","Template":"IGHV4-38-2","Group":"IGHV4-38-2","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-2.958,"Position_3terminus":1,"All_mismatches":"24,21,20,18,17,16,10,8,7,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"atggactggacctggagggtcttc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.58333,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":61.95,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":-3.56,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":2,"_row":"86_openPrimeR2017"}
{"Primer":"Opt5_10","Template":"IGHV4-39","Group":"IGHV4-39","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-1.695,"Position_3terminus":1,"All_mismatches":"23,21,19,18,16,13,9,6,4,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"atggactggacctggagggtcttc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.58333,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":61.95,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":-3.56,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":4,"_row":"87_openPrimeR2017"}
{"Primer":"Opt5_10","Template":"IGHV4-4","Group":"IGHV4-4","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-1.695,"Position_3terminus":1,"All_mismatches":"23,21,19,18,16,13,9,7,6,5,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"atggactggacctggagggtcttc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.58333,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":61.95,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":-3.56,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"88_openPrimeR2017"}
{"Primer":"Opt5_10","Template":"IGHV4-59","Group":"IGHV4-59","Number_of_mismatches":12,"primer_efficiency":0,"annealing_DeltaG":-3.127,"Position_3terminus":1,"All_mismatches":"24,20,19,18,17,16,15,12,10,9,3,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"atggactggacctggagggtcttc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.58333,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":61.95,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":-3.56,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":2,"_row":"89_openPrimeR2017"}
{"Primer":"Opt5_10","Template":"IGHV4-61","Group":"IGHV4-61","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-2.536,"Position_3terminus":3,"All_mismatches":"23,21,19,18,16,13,9,6,5,4,3","Worst_Case_MismatchLocal":"4,4,4,4,4,4,4,4,4,4,4","Primer_Sequence":"atggactggacctggagggtcttc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.58333,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":61.95,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":-3.56,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":4,"Number_of_mismatches_hexamer":4,"_row":"90_openPrimeR2017"}
{"Primer":"Opt5_10","Template":"IGHV5-10-1","Group":"IGHV5-10-1","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-3.517,"Position_3terminus":2,"All_mismatches":"23,22,21,14,12,10,9,4,3,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"atggactggacctggagggtcttc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.58333,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":61.95,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":-3.56,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":3,"_row":"91_openPrimeR2017"}
{"Primer":"Opt5_10","Template":"IGHV5-51","Group":"IGHV5-51","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-4.414,"Position_3terminus":2,"All_mismatches":"24,23,21,20,17,16,15,9,8,6,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"atggactggacctggagggtcttc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.58333,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":61.95,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":-3.56,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":2,"_row":"92_openPrimeR2017"}
{"Primer":"Opt5_10","Template":"IGHV6-1","Group":"IGHV6-1","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-2.889,"Position_3terminus":1,"All_mismatches":"24,20,18,17,16,15,13,9,7,3,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"atggactggacctggagggtcttc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.58333,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":61.95,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":-3.56,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":2,"_row":"93_openPrimeR2017"}
{"Primer":"Opt5_10","Template":"IGHV7-4-1","Group":"IGHV7-4-1","Number_of_mismatches":2,"primer_efficiency":0,"annealing_DeltaG":-13.564,"Position_3terminus":3,"All_mismatches":"6,3","Worst_Case_MismatchLocal":"4,4","Primer_Sequence":"atggactggacctggagggtcttc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":1,"gc_ratio_fw":0.58333,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":61.95,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":-3.56,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":4,"Number_of_mismatches_hexamer":2,"_row":"94_openPrimeR2017"}
{"Primer":"Opt5_11","Template":"IGHV1-2","Group":"IGHV1-2","Number_of_mismatches":12,"primer_efficiency":0,"annealing_DeltaG":-2.414,"Position_3terminus":2,"All_mismatches":"23,22,21,19,18,16,15,12,6,5,3,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"tctgtctccttcctcatcttcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":25,"gc_clamp_fw":2,"gc_ratio_fw":0.52,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.26,"Self_Dimer_DeltaG":0.407,"Structure_deltaG_fw":0,"primer_coverage":25,"primer_specificity":0.34722,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":4,"_row":"95_openPrimeR2017"}
{"Primer":"Opt5_11","Template":"IGHV1-24","Group":"IGHV1-24","Number_of_mismatches":12,"primer_efficiency":0,"annealing_DeltaG":-1.852,"Position_3terminus":1,"All_mismatches":"23,22,19,15,13,12,11,9,7,6,3,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"tctgtctccttcctcatcttcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":25,"gc_clamp_fw":2,"gc_ratio_fw":0.52,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.26,"Self_Dimer_DeltaG":0.407,"Structure_deltaG_fw":0,"primer_coverage":25,"primer_specificity":0.34722,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"96_openPrimeR2017"}
{"Primer":"Opt5_11","Template":"IGHV1-3","Group":"IGHV1-3","Number_of_mismatches":12,"primer_efficiency":0,"annealing_DeltaG":-3.561,"Position_3terminus":2,"All_mismatches":"25,23,22,21,20,16,9,8,7,6,3,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"tctgtctccttcctcatcttcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":25,"gc_clamp_fw":2,"gc_ratio_fw":0.52,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.26,"Self_Dimer_DeltaG":0.407,"Structure_deltaG_fw":0,"primer_coverage":25,"primer_specificity":0.34722,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":3,"_row":"97_openPrimeR2017"}
{"Primer":"Opt5_11","Template":"IGHV1-45","Group":"IGHV1-45","Number_of_mismatches":12,"primer_efficiency":0,"annealing_DeltaG":-1.003,"Position_3terminus":2,"All_mismatches":"25,24,21,19,18,16,15,12,9,8,6,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"tctgtctccttcctcatcttcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":25,"gc_clamp_fw":2,"gc_ratio_fw":0.52,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.26,"Self_Dimer_DeltaG":0.407,"Structure_deltaG_fw":0,"primer_coverage":25,"primer_specificity":0.34722,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":2,"_row":"98_openPrimeR2017"}
{"Primer":"Opt5_11","Template":"IGHV1-46","Group":"IGHV1-46","Number_of_mismatches":12,"primer_efficiency":0,"annealing_DeltaG":-2.29,"Position_3terminus":1,"All_mismatches":"21,20,18,15,14,11,10,9,8,7,3,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"tctgtctccttcctcatcttcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":25,"gc_clamp_fw":2,"gc_ratio_fw":0.52,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.26,"Self_Dimer_DeltaG":0.407,"Structure_deltaG_fw":0,"primer_coverage":25,"primer_specificity":0.34722,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":2,"_row":"99_openPrimeR2017"}
{"Primer":"Opt5_11","Template":"IGHV2-26","Group":"IGHV2-26","Number_of_mismatches":11,"primer_efficiency":2.38787e-07,"annealing_DeltaG":-4.168,"Position_3terminus":1,"All_mismatches":"25,21,20,19,17,16,15,12,10,9,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"tctgtctccttcctcatcttcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Unamplified","primer_length_fw":25,"gc_clamp_fw":2,"gc_ratio_fw":0.52,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.26,"Self_Dimer_DeltaG":0.407,"Structure_deltaG_fw":0,"primer_coverage":25,"primer_specificity":0.34722,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":1,"_row":"100_openPrimeR2017"}
{"Primer":"Opt5_11","Template":"IGHV2-5","Group":"IGHV2-5","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-1.386,"Position_3terminus":1,"All_mismatches":"24,23,18,17,15,12,11,7,6,4,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"tctgtctccttcctcatcttcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":25,"gc_clamp_fw":2,"gc_ratio_fw":0.52,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.26,"Self_Dimer_DeltaG":0.407,"Structure_deltaG_fw":0,"primer_coverage":25,"primer_specificity":0.34722,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"101_openPrimeR2017"}
{"Primer":"Opt5_11","Template":"IGHV2-70","Group":"IGHV2-70","Number_of_mismatches":12,"primer_efficiency":0,"annealing_DeltaG":-2.081,"Position_3terminus":2,"All_mismatches":"25,19,17,15,13,12,11,10,5,4,3,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"tctgtctccttcctcatcttcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":25,"gc_clamp_fw":2,"gc_ratio_fw":0.52,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.26,"Self_Dimer_DeltaG":0.407,"Structure_deltaG_fw":0,"primer_coverage":25,"primer_specificity":0.34722,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":4,"_row":"102_openPrimeR2017"}
{"Primer":"Opt5_11","Template":"IGHV3-30","Group":"IGHV3-30","Number_of_mismatches":12,"primer_efficiency":0,"annealing_DeltaG":-2.197,"Position_3terminus":1,"All_mismatches":"24,22,21,17,14,10,8,6,5,4,3,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"tctgtctccttcctcatcttcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":5,"Experimental_Coverage":"Unamplified","primer_length_fw":25,"gc_clamp_fw":2,"gc_ratio_fw":0.52,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.26,"Self_Dimer_DeltaG":0.407,"Structure_deltaG_fw":0,"primer_coverage":25,"primer_specificity":0.34722,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":5,"_row":"103_openPrimeR2017"}
{"Primer":"Opt5_11","Template":"IGHV3-30-3","Group":"IGHV3-30-3","Number_of_mismatches":12,"primer_efficiency":0,"annealing_DeltaG":-2.197,"Position_3terminus":1,"All_mismatches":"24,22,21,17,14,10,8,6,5,4,3,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"tctgtctccttcctcatcttcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":5,"Experimental_Coverage":"Unamplified","primer_length_fw":25,"gc_clamp_fw":2,"gc_ratio_fw":0.52,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.26,"Self_Dimer_DeltaG":0.407,"Structure_deltaG_fw":0,"primer_coverage":25,"primer_specificity":0.34722,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":5,"_row":"104_openPrimeR2017"}
{"Primer":"Opt5_11","Template":"IGHV3-33","Group":"IGHV3-33","Number_of_mismatches":12,"primer_efficiency":0,"annealing_DeltaG":-2.197,"Position_3terminus":1,"All_mismatches":"24,22,21,17,14,10,8,6,5,4,3,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"tctgtctccttcctcatcttcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":5,"Experimental_Coverage":"Unamplified","primer_length_fw":25,"gc_clamp_fw":2,"gc_ratio_fw":0.52,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.26,"Self_Dimer_DeltaG":0.407,"Structure_deltaG_fw":0,"primer_coverage":25,"primer_specificity":0.34722,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":5,"_row":"105_openPrimeR2017"}
{"Primer":"Opt5_11","Template":"IGHV3-64D","Group":"IGHV3-64D","Number_of_mismatches":12,"primer_efficiency":0,"annealing_DeltaG":-3.135,"Position_3terminus":1,"All_mismatches":"25,21,20,19,17,16,11,10,7,6,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"tctgtctccttcctcatcttcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":25,"gc_clamp_fw":2,"gc_ratio_fw":0.52,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.26,"Self_Dimer_DeltaG":0.407,"Structure_deltaG_fw":0,"primer_coverage":25,"primer_specificity":0.34722,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"106_openPrimeR2017"}
{"Primer":"Opt5_11","Template":"IGHV3-74","Group":"IGHV3-74","Number_of_mismatches":12,"primer_efficiency":0,"annealing_DeltaG":-2.29,"Position_3terminus":1,"All_mismatches":"24,22,18,14,11,7,6,5,4,3,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"tctgtctccttcctcatcttcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":6,"Experimental_Coverage":"Unamplified","primer_length_fw":25,"gc_clamp_fw":2,"gc_ratio_fw":0.52,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.26,"Self_Dimer_DeltaG":0.407,"Structure_deltaG_fw":0,"primer_coverage":25,"primer_specificity":0.34722,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":6,"_row":"107_openPrimeR2017"}
{"Primer":"Opt5_11","Template":"IGHV4-28","Group":"IGHV4-28","Number_of_mismatches":12,"primer_efficiency":0,"annealing_DeltaG":-3.992,"Position_3terminus":1,"All_mismatches":"25,24,23,22,20,18,17,13,10,7,5,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"tctgtctccttcctcatcttcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":25,"gc_clamp_fw":2,"gc_ratio_fw":0.52,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.26,"Self_Dimer_DeltaG":0.407,"Structure_deltaG_fw":0,"primer_coverage":25,"primer_specificity":0.34722,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":2,"_row":"108_openPrimeR2017"}
{"Primer":"Opt5_11","Template":"IGHV4-30-2","Group":"IGHV4-30-2","Number_of_mismatches":12,"primer_efficiency":0,"annealing_DeltaG":-2.437,"Position_3terminus":1,"All_mismatches":"25,24,21,20,19,18,14,11,9,8,7,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"tctgtctccttcctcatcttcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Unamplified","primer_length_fw":25,"gc_clamp_fw":2,"gc_ratio_fw":0.52,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.26,"Self_Dimer_DeltaG":0.407,"Structure_deltaG_fw":0,"primer_coverage":25,"primer_specificity":0.34722,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":1,"_row":"109_openPrimeR2017"}
{"Primer":"Opt5_11","Template":"IGHV4-31","Group":"IGHV4-31","Number_of_mismatches":12,"primer_efficiency":0,"annealing_DeltaG":-2.522,"Position_3terminus":1,"All_mismatches":"24,22,20,19,18,17,16,11,10,9,7,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"tctgtctccttcctcatcttcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Unamplified","primer_length_fw":25,"gc_clamp_fw":2,"gc_ratio_fw":0.52,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.26,"Self_Dimer_DeltaG":0.407,"Structure_deltaG_fw":0,"primer_coverage":25,"primer_specificity":0.34722,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":1,"_row":"110_openPrimeR2017"}
{"Primer":"Opt5_11","Template":"IGHV4-34","Group":"IGHV4-34","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-3.992,"Position_3terminus":1,"All_mismatches":"25,24,23,22,20,18,17,13,10,7,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"tctgtctccttcctcatcttcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Unamplified","primer_length_fw":25,"gc_clamp_fw":2,"gc_ratio_fw":0.52,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.26,"Self_Dimer_DeltaG":0.407,"Structure_deltaG_fw":0,"primer_coverage":25,"primer_specificity":0.34722,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":1,"_row":"111_openPrimeR2017"}
{"Primer":"Opt5_11","Template":"IGHV4-38-2","Group":"IGHV4-38-2","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-3.651,"Position_3terminus":1,"All_mismatches":"25,20,19,18,17,10,8,7,5,4,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"tctgtctccttcctcatcttcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":25,"gc_clamp_fw":2,"gc_ratio_fw":0.52,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.26,"Self_Dimer_DeltaG":0.407,"Structure_deltaG_fw":0,"primer_coverage":25,"primer_specificity":0.34722,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"112_openPrimeR2017"}
{"Primer":"Opt5_11","Template":"IGHV4-39","Group":"IGHV4-39","Number_of_mismatches":12,"primer_efficiency":0,"annealing_DeltaG":-3.992,"Position_3terminus":1,"All_mismatches":"25,24,23,22,20,18,17,13,10,7,5,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"tctgtctccttcctcatcttcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":25,"gc_clamp_fw":2,"gc_ratio_fw":0.52,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.26,"Self_Dimer_DeltaG":0.407,"Structure_deltaG_fw":0,"primer_coverage":25,"primer_specificity":0.34722,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":2,"_row":"113_openPrimeR2017"}
{"Primer":"Opt5_11","Template":"IGHV4-4","Group":"IGHV4-4","Number_of_mismatches":12,"primer_efficiency":0,"annealing_DeltaG":-1.463,"Position_3terminus":1,"All_mismatches":"24,23,21,20,18,14,11,9,8,7,4,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"tctgtctccttcctcatcttcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":25,"gc_clamp_fw":2,"gc_ratio_fw":0.52,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.26,"Self_Dimer_DeltaG":0.407,"Structure_deltaG_fw":0,"primer_coverage":25,"primer_specificity":0.34722,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":2,"_row":"114_openPrimeR2017"}
{"Primer":"Opt5_11","Template":"IGHV4-59","Group":"IGHV4-59","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-3.559,"Position_3terminus":1,"All_mismatches":"25,24,22,20,18,17,13,10,8,7,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"tctgtctccttcctcatcttcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Unamplified","primer_length_fw":25,"gc_clamp_fw":2,"gc_ratio_fw":0.52,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.26,"Self_Dimer_DeltaG":0.407,"Structure_deltaG_fw":0,"primer_coverage":25,"primer_specificity":0.34722,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":1,"_row":"115_openPrimeR2017"}
{"Primer":"Opt5_11","Template":"IGHV4-61","Group":"IGHV4-61","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-3.992,"Position_3terminus":1,"All_mismatches":"25,24,23,22,20,18,17,13,10,7,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"tctgtctccttcctcatcttcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Unamplified","primer_length_fw":25,"gc_clamp_fw":2,"gc_ratio_fw":0.52,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.26,"Self_Dimer_DeltaG":0.407,"Structure_deltaG_fw":0,"primer_coverage":25,"primer_specificity":0.34722,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":1,"_row":"116_openPrimeR2017"}
{"Primer":"Opt5_11","Template":"IGHV5-10-1","Group":"IGHV5-10-1","Number_of_mismatches":9,"primer_efficiency":4.79179e-06,"annealing_DeltaG":-2.716,"Position_3terminus":2,"All_mismatches":"23,22,21,19,16,10,9,7,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5","Primer_Sequence":"tctgtctccttcctcatcttcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Unamplified","primer_length_fw":25,"gc_clamp_fw":2,"gc_ratio_fw":0.52,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.26,"Self_Dimer_DeltaG":0.407,"Structure_deltaG_fw":0,"primer_coverage":25,"primer_specificity":0.34722,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":1,"_row":"117_openPrimeR2017"}
{"Primer":"Opt5_11","Template":"IGHV5-51","Group":"IGHV5-51","Number_of_mismatches":9,"primer_efficiency":4.79179e-06,"annealing_DeltaG":-2.716,"Position_3terminus":2,"All_mismatches":"23,22,21,19,16,10,9,7,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5","Primer_Sequence":"tctgtctccttcctcatcttcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Unamplified","primer_length_fw":25,"gc_clamp_fw":2,"gc_ratio_fw":0.52,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.26,"Self_Dimer_DeltaG":0.407,"Structure_deltaG_fw":0,"primer_coverage":25,"primer_specificity":0.34722,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":1,"_row":"118_openPrimeR2017"}
{"Primer":"Opt5_11","Template":"IGHV6-1","Group":"IGHV6-1","Number_of_mismatches":0,"primer_efficiency":0.99998,"annealing_DeltaG":-17.096,"All_mismatches":"NA","Worst_Case_MismatchLocal":"0","Primer_Sequence":"tctgtctccttcctcatcttcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":25,"gc_clamp_fw":2,"gc_ratio_fw":0.52,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.26,"Self_Dimer_DeltaG":0.407,"Structure_deltaG_fw":0,"primer_coverage":25,"primer_specificity":0.34722,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"119_openPrimeR2017"}
{"Primer":"Opt5_12","Template":"IGHV1-18","Group":"IGHV1-18","Number_of_mismatches":5,"primer_efficiency":0.02157,"annealing_DeltaG":-8.651,"Position_3terminus":4,"All_mismatches":"17,16,10,9,4","Worst_Case_MismatchLocal":"3,3,3,3,3","Primer_Sequence":"ggactggatttggagggtcctcttc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Amplified","primer_length_fw":25,"gc_clamp_fw":1,"gc_ratio_fw":0.56,"no_runs_fw":3,"no_repeats_fw":2,"melting_temp":60.55,"Self_Dimer_DeltaG":-2.225,"Structure_deltaG_fw":-3.23,"primer_coverage":39,"primer_specificity":0.45882,"Run":"openPrimeR2017","Position_3terminusLocal":3,"Number_of_mismatches_hexamer":1,"_row":"120_openPrimeR2017"}
{"Primer":"Opt5_12","Template":"IGHV1-2","Group":"IGHV1-2","Number_of_mismatches":3,"primer_efficiency":0.90189,"annealing_DeltaG":-11.824,"Position_3terminus":9,"All_mismatches":"17,16,9","Worst_Case_MismatchLocal":"0,0,0","Primer_Sequence":"ggactggatttggagggtcctcttc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":25,"gc_clamp_fw":1,"gc_ratio_fw":0.56,"no_runs_fw":3,"no_repeats_fw":2,"melting_temp":60.55,"Self_Dimer_DeltaG":-2.225,"Structure_deltaG_fw":-3.23,"primer_coverage":39,"primer_specificity":0.45882,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"121_openPrimeR2017"}
{"Primer":"Opt5_12","Template":"IGHV1-24","Group":"IGHV1-24","Number_of_mismatches":4,"primer_efficiency":0.32714,"annealing_DeltaG":-9.867,"Position_3terminus":9,"All_mismatches":"19,17,16,9","Worst_Case_MismatchLocal":"0,0,0,0","Primer_Sequence":"ggactggatttggagggtcctcttc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":25,"gc_clamp_fw":1,"gc_ratio_fw":0.56,"no_runs_fw":3,"no_repeats_fw":2,"melting_temp":60.55,"Self_Dimer_DeltaG":-2.225,"Structure_deltaG_fw":-3.23,"primer_coverage":39,"primer_specificity":0.45882,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"122_openPrimeR2017"}
{"Primer":"Opt5_12","Template":"IGHV1-3","Group":"IGHV1-3","Number_of_mismatches":4,"primer_efficiency":0.02599,"annealing_DeltaG":-11.783,"Position_3terminus":1,"All_mismatches":"17,16,9,1","Worst_Case_MismatchLocal":"6,6,6,6","Primer_Sequence":"ggactggatttggagggtcctcttc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Amplified","primer_length_fw":25,"gc_clamp_fw":1,"gc_ratio_fw":0.56,"no_runs_fw":3,"no_repeats_fw":2,"melting_temp":60.55,"Self_Dimer_DeltaG":-2.225,"Structure_deltaG_fw":-3.23,"primer_coverage":39,"primer_specificity":0.45882,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":1,"_row":"123_openPrimeR2017"}
{"Primer":"Opt5_12","Template":"IGHV1-45","Group":"IGHV1-45","Number_of_mismatches":4,"primer_efficiency":0.33667,"annealing_DeltaG":-9.895,"Position_3terminus":9,"All_mismatches":"17,16,10,9","Worst_Case_MismatchLocal":"0,0,0,0","Primer_Sequence":"ggactggatttggagggtcctcttc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":25,"gc_clamp_fw":1,"gc_ratio_fw":0.56,"no_runs_fw":3,"no_repeats_fw":2,"melting_temp":60.55,"Self_Dimer_DeltaG":-2.225,"Structure_deltaG_fw":-3.23,"primer_coverage":39,"primer_specificity":0.45882,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"124_openPrimeR2017"}
{"Primer":"Opt5_12","Template":"IGHV1-46","Group":"IGHV1-46","Number_of_mismatches":4,"primer_efficiency":0,"annealing_DeltaG":-10.165,"Position_3terminus":2,"All_mismatches":"17,16,6,2","Worst_Case_MismatchLocal":"5,5,5,5","Primer_Sequence":"ggactggatttggagggtcctcttc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Amplified","primer_length_fw":25,"gc_clamp_fw":1,"gc_ratio_fw":0.56,"no_runs_fw":3,"no_repeats_fw":2,"melting_temp":60.55,"Self_Dimer_DeltaG":-2.225,"Structure_deltaG_fw":-3.23,"primer_coverage":39,"primer_specificity":0.45882,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":2,"_row":"125_openPrimeR2017"}
{"Primer":"Opt5_12","Template":"IGHV1-58","Group":"IGHV1-58","Number_of_mismatches":1,"primer_efficiency":0.99908,"annealing_DeltaG":-14.894,"Position_3terminus":9,"All_mismatches":"9","Worst_Case_MismatchLocal":"0","Primer_Sequence":"ggactggatttggagggtcctcttc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":25,"gc_clamp_fw":1,"gc_ratio_fw":0.56,"no_runs_fw":3,"no_repeats_fw":2,"melting_temp":60.55,"Self_Dimer_DeltaG":-2.225,"Structure_deltaG_fw":-3.23,"primer_coverage":39,"primer_specificity":0.45882,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"126_openPrimeR2017"}
{"Primer":"Opt5_12","Template":"IGHV1-69","Group":"IGHV1-69","Number_of_mismatches":4,"primer_efficiency":0.02695,"annealing_DeltaG":-12.291,"Position_3terminus":1,"All_mismatches":"17,16,9,1","Worst_Case_MismatchLocal":"6,6,6,6","Primer_Sequence":"ggactggatttggagggtcctcttc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Amplified","primer_length_fw":25,"gc_clamp_fw":1,"gc_ratio_fw":0.56,"no_runs_fw":3,"no_repeats_fw":2,"melting_temp":60.55,"Self_Dimer_DeltaG":-2.225,"Structure_deltaG_fw":-3.23,"primer_coverage":39,"primer_specificity":0.45882,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":1,"_row":"127_openPrimeR2017"}
{"Primer":"Opt5_12","Template":"IGHV1-69-2","Group":"IGHV1-69-2","Number_of_mismatches":5,"primer_efficiency":0.00654,"annealing_DeltaG":-8.274,"Position_3terminus":3,"All_mismatches":"19,17,16,9,3","Worst_Case_MismatchLocal":"4,4,4,4,4","Primer_Sequence":"ggactggatttggagggtcctcttc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Amplified","primer_length_fw":25,"gc_clamp_fw":1,"gc_ratio_fw":0.56,"no_runs_fw":3,"no_repeats_fw":2,"melting_temp":60.55,"Self_Dimer_DeltaG":-2.225,"Structure_deltaG_fw":-3.23,"primer_coverage":39,"primer_specificity":0.45882,"Run":"openPrimeR2017","Position_3terminusLocal":4,"Number_of_mismatches_hexamer":1,"_row":"128_openPrimeR2017"}
{"Primer":"Opt5_12","Template":"IGHV1-8","Group":"IGHV1-8","Number_of_mismatches":3,"primer_efficiency":0.90189,"annealing_DeltaG":-11.824,"Position_3terminus":9,"All_mismatches":"17,16,9","Worst_Case_MismatchLocal":"0,0,0","Primer_Sequence":"ggactggatttggagggtcctcttc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":25,"gc_clamp_fw":1,"gc_ratio_fw":0.56,"no_runs_fw":3,"no_repeats_fw":2,"melting_temp":60.55,"Self_Dimer_DeltaG":-2.225,"Structure_deltaG_fw":-3.23,"primer_coverage":39,"primer_specificity":0.45882,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"129_openPrimeR2017"}
{"Primer":"Opt5_12","Template":"IGHV2-26","Group":"IGHV2-26","Number_of_mismatches":12,"primer_efficiency":0,"annealing_DeltaG":-1.85,"Position_3terminus":1,"All_mismatches":"25,22,20,19,16,15,10,8,6,5,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"ggactggatttggagggtcctcttc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Amplified","primer_length_fw":25,"gc_clamp_fw":1,"gc_ratio_fw":0.56,"no_runs_fw":3,"no_repeats_fw":2,"melting_temp":60.55,"Self_Dimer_DeltaG":-2.225,"Structure_deltaG_fw":-3.23,"primer_coverage":39,"primer_specificity":0.45882,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":4,"_row":"130_openPrimeR2017"}
{"Primer":"Opt5_12","Template":"IGHV2-5","Group":"IGHV2-5","Number_of_mismatches":12,"primer_efficiency":0,"annealing_DeltaG":-1.85,"Position_3terminus":1,"All_mismatches":"25,22,20,19,16,15,10,8,6,5,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"ggactggatttggagggtcctcttc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":25,"gc_clamp_fw":1,"gc_ratio_fw":0.56,"no_runs_fw":3,"no_repeats_fw":2,"melting_temp":60.55,"Self_Dimer_DeltaG":-2.225,"Structure_deltaG_fw":-3.23,"primer_coverage":39,"primer_specificity":0.45882,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":4,"_row":"131_openPrimeR2017"}
{"Primer":"Opt5_12","Template":"IGHV3-11","Group":"IGHV3-11","Number_of_mismatches":12,"primer_efficiency":0,"annealing_DeltaG":-2.319,"Position_3terminus":1,"All_mismatches":"20,19,18,17,16,10,9,7,6,5,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"ggactggatttggagggtcctcttc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":25,"gc_clamp_fw":1,"gc_ratio_fw":0.56,"no_runs_fw":3,"no_repeats_fw":2,"melting_temp":60.55,"Self_Dimer_DeltaG":-2.225,"Structure_deltaG_fw":-3.23,"primer_coverage":39,"primer_specificity":0.45882,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":4,"_row":"132_openPrimeR2017"}
{"Primer":"Opt5_12","Template":"IGHV3-15","Group":"IGHV3-15","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-2.463,"Position_3terminus":2,"All_mismatches":"25,22,20,19,16,15,10,8,7,3,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"ggactggatttggagggtcctcttc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":25,"gc_clamp_fw":1,"gc_ratio_fw":0.56,"no_runs_fw":3,"no_repeats_fw":2,"melting_temp":60.55,"Self_Dimer_DeltaG":-2.225,"Structure_deltaG_fw":-3.23,"primer_coverage":39,"primer_specificity":0.45882,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":2,"_row":"133_openPrimeR2017"}
{"Primer":"Opt5_12","Template":"IGHV3-21","Group":"IGHV3-21","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-3.74,"Position_3terminus":2,"All_mismatches":"24,23,15,14,13,9,8,7,6,3,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"ggactggatttggagggtcctcttc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":25,"gc_clamp_fw":1,"gc_ratio_fw":0.56,"no_runs_fw":3,"no_repeats_fw":2,"melting_temp":60.55,"Self_Dimer_DeltaG":-2.225,"Structure_deltaG_fw":-3.23,"primer_coverage":39,"primer_specificity":0.45882,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":3,"_row":"134_openPrimeR2017"}
{"Primer":"Opt5_12","Template":"IGHV3-30","Group":"IGHV3-30","Number_of_mismatches":12,"primer_efficiency":0,"annealing_DeltaG":-2.319,"Position_3terminus":1,"All_mismatches":"20,19,18,17,16,10,9,7,6,5,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"ggactggatttggagggtcctcttc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":25,"gc_clamp_fw":1,"gc_ratio_fw":0.56,"no_runs_fw":3,"no_repeats_fw":2,"melting_temp":60.55,"Self_Dimer_DeltaG":-2.225,"Structure_deltaG_fw":-3.23,"primer_coverage":39,"primer_specificity":0.45882,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":4,"_row":"135_openPrimeR2017"}
{"Primer":"Opt5_12","Template":"IGHV3-30-3","Group":"IGHV3-30-3","Number_of_mismatches":12,"primer_efficiency":0,"annealing_DeltaG":-3.486,"Position_3terminus":1,"All_mismatches":"24,17,16,14,12,11,7,6,4,3,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"ggactggatttggagggtcctcttc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":5,"Experimental_Coverage":"Unamplified","primer_length_fw":25,"gc_clamp_fw":1,"gc_ratio_fw":0.56,"no_runs_fw":3,"no_repeats_fw":2,"melting_temp":60.55,"Self_Dimer_DeltaG":-2.225,"Structure_deltaG_fw":-3.23,"primer_coverage":39,"primer_specificity":0.45882,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":5,"_row":"136_openPrimeR2017"}
{"Primer":"Opt5_12","Template":"IGHV3-33","Group":"IGHV3-33","Number_of_mismatches":12,"primer_efficiency":0,"annealing_DeltaG":-2.319,"Position_3terminus":1,"All_mismatches":"20,19,18,17,16,10,9,7,6,5,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"ggactggatttggagggtcctcttc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":25,"gc_clamp_fw":1,"gc_ratio_fw":0.56,"no_runs_fw":3,"no_repeats_fw":2,"melting_temp":60.55,"Self_Dimer_DeltaG":-2.225,"Structure_deltaG_fw":-3.23,"primer_coverage":39,"primer_specificity":0.45882,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":4,"_row":"137_openPrimeR2017"}
{"Primer":"Opt5_12","Template":"IGHV3-43","Group":"IGHV3-43","Number_of_mismatches":12,"primer_efficiency":0,"annealing_DeltaG":-2.904,"Position_3terminus":1,"All_mismatches":"19,18,17,15,12,11,10,9,8,6,4,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"ggactggatttggagggtcctcttc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":25,"gc_clamp_fw":1,"gc_ratio_fw":0.56,"no_runs_fw":3,"no_repeats_fw":2,"melting_temp":60.55,"Self_Dimer_DeltaG":-2.225,"Structure_deltaG_fw":-3.23,"primer_coverage":39,"primer_specificity":0.45882,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"138_openPrimeR2017"}
{"Primer":"Opt5_12","Template":"IGHV3-48","Group":"IGHV3-48","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-3.74,"Position_3terminus":2,"All_mismatches":"24,23,15,14,13,9,8,7,6,3,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"ggactggatttggagggtcctcttc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":25,"gc_clamp_fw":1,"gc_ratio_fw":0.56,"no_runs_fw":3,"no_repeats_fw":2,"melting_temp":60.55,"Self_Dimer_DeltaG":-2.225,"Structure_deltaG_fw":-3.23,"primer_coverage":39,"primer_specificity":0.45882,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":3,"_row":"139_openPrimeR2017"}
{"Primer":"Opt5_12","Template":"IGHV3-49","Group":"IGHV3-49","Number_of_mismatches":12,"primer_efficiency":0,"annealing_DeltaG":-2.904,"Position_3terminus":1,"All_mismatches":"24,23,22,21,18,15,13,12,7,6,3,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"ggactggatttggagggtcctcttc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":25,"gc_clamp_fw":1,"gc_ratio_fw":0.56,"no_runs_fw":3,"no_repeats_fw":2,"melting_temp":60.55,"Self_Dimer_DeltaG":-2.225,"Structure_deltaG_fw":-3.23,"primer_coverage":39,"primer_specificity":0.45882,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"140_openPrimeR2017"}
{"Primer":"Opt5_12","Template":"IGHV3-53","Group":"IGHV3-53","Number_of_mismatches":12,"primer_efficiency":0,"annealing_DeltaG":-4.469,"Position_3terminus":1,"All_mismatches":"18,17,14,13,12,11,7,6,4,3,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"ggactggatttggagggtcctcttc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":5,"Experimental_Coverage":"Unamplified","primer_length_fw":25,"gc_clamp_fw":1,"gc_ratio_fw":0.56,"no_runs_fw":3,"no_repeats_fw":2,"melting_temp":60.55,"Self_Dimer_DeltaG":-2.225,"Structure_deltaG_fw":-3.23,"primer_coverage":39,"primer_specificity":0.45882,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":5,"_row":"141_openPrimeR2017"}
{"Primer":"Opt5_12","Template":"IGHV3-64D","Group":"IGHV3-64D","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-3.14,"Position_3terminus":1,"All_mismatches":"24,23,18,16,15,13,12,7,3,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"ggactggatttggagggtcctcttc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":25,"gc_clamp_fw":1,"gc_ratio_fw":0.56,"no_runs_fw":3,"no_repeats_fw":2,"melting_temp":60.55,"Self_Dimer_DeltaG":-2.225,"Structure_deltaG_fw":-3.23,"primer_coverage":39,"primer_specificity":0.45882,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":2,"_row":"142_openPrimeR2017"}
{"Primer":"Opt5_12","Template":"IGHV3-66","Group":"IGHV3-66","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-2.319,"Position_3terminus":1,"All_mismatches":"20,18,17,16,10,9,7,6,5,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"ggactggatttggagggtcctcttc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":25,"gc_clamp_fw":1,"gc_ratio_fw":0.56,"no_runs_fw":3,"no_repeats_fw":2,"melting_temp":60.55,"Self_Dimer_DeltaG":-2.225,"Structure_deltaG_fw":-3.23,"primer_coverage":39,"primer_specificity":0.45882,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":4,"_row":"143_openPrimeR2017"}
{"Primer":"Opt5_12","Template":"IGHV3-7","Group":"IGHV3-7","Number_of_mismatches":12,"primer_efficiency":0,"annealing_DeltaG":-2.74,"Position_3terminus":2,"All_mismatches":"24,23,17,15,14,13,9,8,7,6,3,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"ggactggatttggagggtcctcttc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":25,"gc_clamp_fw":1,"gc_ratio_fw":0.56,"no_runs_fw":3,"no_repeats_fw":2,"melting_temp":60.55,"Self_Dimer_DeltaG":-2.225,"Structure_deltaG_fw":-3.23,"primer_coverage":39,"primer_specificity":0.45882,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":3,"_row":"144_openPrimeR2017"}
{"Primer":"Opt5_12","Template":"IGHV3-72","Group":"IGHV3-72","Number_of_mismatches":12,"primer_efficiency":0,"annealing_DeltaG":-2.319,"Position_3terminus":1,"All_mismatches":"20,19,18,17,16,10,9,7,6,5,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"ggactggatttggagggtcctcttc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":25,"gc_clamp_fw":1,"gc_ratio_fw":0.56,"no_runs_fw":3,"no_repeats_fw":2,"melting_temp":60.55,"Self_Dimer_DeltaG":-2.225,"Structure_deltaG_fw":-3.23,"primer_coverage":39,"primer_specificity":0.45882,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":4,"_row":"145_openPrimeR2017"}
{"Primer":"Opt5_12","Template":"IGHV3-73","Group":"IGHV3-73","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-5.348,"Position_3terminus":2,"All_mismatches":"16,15,14,12,11,10,8,7,4,3,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"ggactggatttggagggtcctcttc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":25,"gc_clamp_fw":1,"gc_ratio_fw":0.56,"no_runs_fw":3,"no_repeats_fw":2,"melting_temp":60.55,"Self_Dimer_DeltaG":-2.225,"Structure_deltaG_fw":-3.23,"primer_coverage":39,"primer_specificity":0.45882,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":3,"_row":"146_openPrimeR2017"}
{"Primer":"Opt5_12","Template":"IGHV3-74","Group":"IGHV3-74","Number_of_mismatches":12,"primer_efficiency":0,"annealing_DeltaG":-1.423,"Position_3terminus":2,"All_mismatches":"24,22,21,19,17,16,12,9,8,5,3,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"ggactggatttggagggtcctcttc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":25,"gc_clamp_fw":1,"gc_ratio_fw":0.56,"no_runs_fw":3,"no_repeats_fw":2,"melting_temp":60.55,"Self_Dimer_DeltaG":-2.225,"Structure_deltaG_fw":-3.23,"primer_coverage":39,"primer_specificity":0.45882,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":3,"_row":"147_openPrimeR2017"}
{"Primer":"Opt5_12","Template":"IGHV3-9","Group":"IGHV3-9","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-4.044,"Position_3terminus":1,"All_mismatches":"23,22,21,20,17,16,11,10,9,4,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"ggactggatttggagggtcctcttc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":25,"gc_clamp_fw":1,"gc_ratio_fw":0.56,"no_runs_fw":3,"no_repeats_fw":2,"melting_temp":60.55,"Self_Dimer_DeltaG":-2.225,"Structure_deltaG_fw":-3.23,"primer_coverage":39,"primer_specificity":0.45882,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":2,"_row":"148_openPrimeR2017"}
{"Primer":"Opt5_12","Template":"IGHV4-28","Group":"IGHV4-28","Number_of_mismatches":12,"primer_efficiency":0,"annealing_DeltaG":-3.544,"Position_3terminus":1,"All_mismatches":"23,16,15,12,11,9,8,5,4,3,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"ggactggatttggagggtcctcttc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":5,"Experimental_Coverage":"Unamplified","primer_length_fw":25,"gc_clamp_fw":1,"gc_ratio_fw":0.56,"no_runs_fw":3,"no_repeats_fw":2,"melting_temp":60.55,"Self_Dimer_DeltaG":-2.225,"Structure_deltaG_fw":-3.23,"primer_coverage":39,"primer_specificity":0.45882,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":5,"_row":"149_openPrimeR2017"}
{"Primer":"Opt5_12","Template":"IGHV4-30-2","Group":"IGHV4-30-2","Number_of_mismatches":12,"primer_efficiency":0,"annealing_DeltaG":-4.16,"Position_3terminus":2,"All_mismatches":"25,23,22,21,20,19,18,15,14,10,4,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"ggactggatttggagggtcctcttc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":25,"gc_clamp_fw":1,"gc_ratio_fw":0.56,"no_runs_fw":3,"no_repeats_fw":2,"melting_temp":60.55,"Self_Dimer_DeltaG":-2.225,"Structure_deltaG_fw":-3.23,"primer_coverage":39,"primer_specificity":0.45882,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":2,"_row":"150_openPrimeR2017"}
{"Primer":"Opt5_12","Template":"IGHV4-31","Group":"IGHV4-31","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-4.16,"Position_3terminus":2,"All_mismatches":"24,22,20,19,18,15,14,10,4,3,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"ggactggatttggagggtcctcttc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":25,"gc_clamp_fw":1,"gc_ratio_fw":0.56,"no_runs_fw":3,"no_repeats_fw":2,"melting_temp":60.55,"Self_Dimer_DeltaG":-2.225,"Structure_deltaG_fw":-3.23,"primer_coverage":39,"primer_specificity":0.45882,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":3,"_row":"151_openPrimeR2017"}
{"Primer":"Opt5_12","Template":"IGHV4-34","Group":"IGHV4-34","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-1.721,"Position_3terminus":2,"All_mismatches":"22,19,18,15,14,10,6,4,3,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"ggactggatttggagggtcctcttc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":25,"gc_clamp_fw":1,"gc_ratio_fw":0.56,"no_runs_fw":3,"no_repeats_fw":2,"melting_temp":60.55,"Self_Dimer_DeltaG":-2.225,"Structure_deltaG_fw":-3.23,"primer_coverage":39,"primer_specificity":0.45882,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":4,"_row":"152_openPrimeR2017"}
{"Primer":"Opt5_12","Template":"IGHV4-38-2","Group":"IGHV4-38-2","Number_of_mismatches":12,"primer_efficiency":0,"annealing_DeltaG":-3.544,"Position_3terminus":1,"All_mismatches":"23,16,15,12,11,9,8,5,4,3,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"ggactggatttggagggtcctcttc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":5,"Experimental_Coverage":"Unamplified","primer_length_fw":25,"gc_clamp_fw":1,"gc_ratio_fw":0.56,"no_runs_fw":3,"no_repeats_fw":2,"melting_temp":60.55,"Self_Dimer_DeltaG":-2.225,"Structure_deltaG_fw":-3.23,"primer_coverage":39,"primer_specificity":0.45882,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":5,"_row":"153_openPrimeR2017"}
{"Primer":"Opt5_12","Template":"IGHV4-59","Group":"IGHV4-59","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-4.16,"Position_3terminus":2,"All_mismatches":"24,22,20,19,18,15,14,10,4,3,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"ggactggatttggagggtcctcttc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":25,"gc_clamp_fw":1,"gc_ratio_fw":0.56,"no_runs_fw":3,"no_repeats_fw":2,"melting_temp":60.55,"Self_Dimer_DeltaG":-2.225,"Structure_deltaG_fw":-3.23,"primer_coverage":39,"primer_specificity":0.45882,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":3,"_row":"154_openPrimeR2017"}
{"Primer":"Opt5_12","Template":"IGHV4-61","Group":"IGHV4-61","Number_of_mismatches":12,"primer_efficiency":0,"annealing_DeltaG":-2.027,"Position_3terminus":3,"All_mismatches":"24,22,21,20,19,18,16,12,11,10,9,3","Worst_Case_MismatchLocal":"4,4,4,4,4,4,4,4,4,4,4,4","Primer_Sequence":"ggactggatttggagggtcctcttc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Unamplified","primer_length_fw":25,"gc_clamp_fw":1,"gc_ratio_fw":0.56,"no_runs_fw":3,"no_repeats_fw":2,"melting_temp":60.55,"Self_Dimer_DeltaG":-2.225,"Structure_deltaG_fw":-3.23,"primer_coverage":39,"primer_specificity":0.45882,"Run":"openPrimeR2017","Position_3terminusLocal":4,"Number_of_mismatches_hexamer":1,"_row":"155_openPrimeR2017"}
{"Primer":"Opt5_12","Template":"IGHV5-10-1","Group":"IGHV5-10-1","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-4.422,"Position_3terminus":1,"All_mismatches":"25,24,16,15,13,12,7,5,3,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"ggactggatttggagggtcctcttc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":25,"gc_clamp_fw":1,"gc_ratio_fw":0.56,"no_runs_fw":3,"no_repeats_fw":2,"melting_temp":60.55,"Self_Dimer_DeltaG":-2.225,"Structure_deltaG_fw":-3.23,"primer_coverage":39,"primer_specificity":0.45882,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":4,"_row":"156_openPrimeR2017"}
{"Primer":"Opt5_12","Template":"IGHV6-1","Group":"IGHV6-1","Number_of_mismatches":12,"primer_efficiency":0,"annealing_DeltaG":-1.403,"Position_3terminus":1,"All_mismatches":"25,22,20,19,16,15,10,8,6,5,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"ggactggatttggagggtcctcttc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":25,"gc_clamp_fw":1,"gc_ratio_fw":0.56,"no_runs_fw":3,"no_repeats_fw":2,"melting_temp":60.55,"Self_Dimer_DeltaG":-2.225,"Structure_deltaG_fw":-3.23,"primer_coverage":39,"primer_specificity":0.45882,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":4,"_row":"157_openPrimeR2017"}
{"Primer":"Opt5_12","Template":"IGHV7-4-1","Group":"IGHV7-4-1","Number_of_mismatches":3,"primer_efficiency":0.90189,"annealing_DeltaG":-11.824,"Position_3terminus":9,"All_mismatches":"17,16,9","Worst_Case_MismatchLocal":"0,0,0","Primer_Sequence":"ggactggatttggagggtcctcttc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":25,"gc_clamp_fw":1,"gc_ratio_fw":0.56,"no_runs_fw":3,"no_repeats_fw":2,"melting_temp":60.55,"Self_Dimer_DeltaG":-2.225,"Structure_deltaG_fw":-3.23,"primer_coverage":39,"primer_specificity":0.45882,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"158_openPrimeR2017"}
{"Primer":"Opt5_13","Template":"IGHV1-18","Group":"IGHV1-18","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-3.18,"Position_3terminus":2,"All_mismatches":"19,16,10,9,8,7,6,5,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5","Primer_Sequence":"gctccgctgggttttccttg","Terminal_Dinucleotide":"tg","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":20,"gc_clamp_fw":1,"gc_ratio_fw":0.6,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":58.65,"Self_Dimer_DeltaG":0.406,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":3,"_row":"159_openPrimeR2017"}
{"Primer":"Opt5_13","Template":"IGHV1-2","Group":"IGHV1-2","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-4.479,"Position_3terminus":1,"All_mismatches":"20,19,15,11,9,8,7,3,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6","Primer_Sequence":"gctccgctgggttttccttg","Terminal_Dinucleotide":"tg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":20,"gc_clamp_fw":1,"gc_ratio_fw":0.6,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":58.65,"Self_Dimer_DeltaG":0.406,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":2,"_row":"160_openPrimeR2017"}
{"Primer":"Opt5_13","Template":"IGHV1-24","Group":"IGHV1-24","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-1.864,"Position_3terminus":1,"All_mismatches":"18,15,14,13,12,9,7,6,5,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"gctccgctgggttttccttg","Terminal_Dinucleotide":"tg","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":20,"gc_clamp_fw":1,"gc_ratio_fw":0.6,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":58.65,"Self_Dimer_DeltaG":0.406,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"161_openPrimeR2017"}
{"Primer":"Opt5_13","Template":"IGHV1-3","Group":"IGHV1-3","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-1.864,"Position_3terminus":1,"All_mismatches":"19,18,16,14,13,10,8,7,4,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"gctccgctgggttttccttg","Terminal_Dinucleotide":"tg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":20,"gc_clamp_fw":1,"gc_ratio_fw":0.6,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":58.65,"Self_Dimer_DeltaG":0.406,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":2,"_row":"162_openPrimeR2017"}
{"Primer":"Opt5_13","Template":"IGHV1-45","Group":"IGHV1-45","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-2.274,"Position_3terminus":1,"All_mismatches":"20,16,14,13,12,11,9,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6","Primer_Sequence":"gctccgctgggttttccttg","Terminal_Dinucleotide":"tg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":20,"gc_clamp_fw":1,"gc_ratio_fw":0.6,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":58.65,"Self_Dimer_DeltaG":0.406,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":2,"_row":"163_openPrimeR2017"}
{"Primer":"Opt5_13","Template":"IGHV1-46","Group":"IGHV1-46","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-2.649,"Position_3terminus":1,"All_mismatches":"19,18,16,14,13,8,4,3,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"gctccgctgggttttccttg","Terminal_Dinucleotide":"tg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":20,"gc_clamp_fw":1,"gc_ratio_fw":0.6,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":58.65,"Self_Dimer_DeltaG":0.406,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":4,"_row":"164_openPrimeR2017"}
{"Primer":"Opt5_13","Template":"IGHV1-58","Group":"IGHV1-58","Number_of_mismatches":8,"primer_efficiency":0,"annealing_DeltaG":-3.043,"Position_3terminus":3,"All_mismatches":"16,11,9,8,7,5,4,3","Worst_Case_MismatchLocal":"4,4,4,4,4,4,4,4","Primer_Sequence":"gctccgctgggttttccttg","Terminal_Dinucleotide":"tg","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":20,"gc_clamp_fw":1,"gc_ratio_fw":0.6,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":58.65,"Self_Dimer_DeltaG":0.406,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":4,"Number_of_mismatches_hexamer":3,"_row":"165_openPrimeR2017"}
{"Primer":"Opt5_13","Template":"IGHV1-69","Group":"IGHV1-69","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-1.996,"Position_3terminus":4,"All_mismatches":"19,18,16,14,13,10,8,7,4","Worst_Case_MismatchLocal":"3,3,3,3,3,3,3,3,3","Primer_Sequence":"gctccgctgggttttccttg","Terminal_Dinucleotide":"tg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Unamplified","primer_length_fw":20,"gc_clamp_fw":1,"gc_ratio_fw":0.6,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":58.65,"Self_Dimer_DeltaG":0.406,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":3,"Number_of_mismatches_hexamer":1,"_row":"166_openPrimeR2017"}
{"Primer":"Opt5_13","Template":"IGHV1-69-2","Group":"IGHV1-69-2","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-1.565,"Position_3terminus":3,"All_mismatches":"19,18,16,14,10,9,7,6,5,3","Worst_Case_MismatchLocal":"4,4,4,4,4,4,4,4,4,4","Primer_Sequence":"gctccgctgggttttccttg","Terminal_Dinucleotide":"tg","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":20,"gc_clamp_fw":1,"gc_ratio_fw":0.6,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":58.65,"Self_Dimer_DeltaG":0.406,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":4,"Number_of_mismatches_hexamer":3,"_row":"167_openPrimeR2017"}
{"Primer":"Opt5_13","Template":"IGHV1-8","Group":"IGHV1-8","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-3.18,"Position_3terminus":2,"All_mismatches":"19,18,16,10,9,8,7,6,5,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"gctccgctgggttttccttg","Terminal_Dinucleotide":"tg","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":20,"gc_clamp_fw":1,"gc_ratio_fw":0.6,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":58.65,"Self_Dimer_DeltaG":0.406,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":3,"_row":"168_openPrimeR2017"}
{"Primer":"Opt5_13","Template":"IGHV2-26","Group":"IGHV2-26","Number_of_mismatches":8,"primer_efficiency":0,"annealing_DeltaG":-5.274,"Position_3terminus":1,"All_mismatches":"20,18,17,15,7,4,3,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6","Primer_Sequence":"gctccgctgggttttccttg","Terminal_Dinucleotide":"tg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":20,"gc_clamp_fw":1,"gc_ratio_fw":0.6,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":58.65,"Self_Dimer_DeltaG":0.406,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"169_openPrimeR2017"}
{"Primer":"Opt5_13","Template":"IGHV2-5","Group":"IGHV2-5","Number_of_mismatches":7,"primer_efficiency":0,"annealing_DeltaG":-5.274,"Position_3terminus":1,"All_mismatches":"20,17,15,7,4,3,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6","Primer_Sequence":"gctccgctgggttttccttg","Terminal_Dinucleotide":"tg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":20,"gc_clamp_fw":1,"gc_ratio_fw":0.6,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":58.65,"Self_Dimer_DeltaG":0.406,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"170_openPrimeR2017"}
{"Primer":"Opt5_13","Template":"IGHV2-70","Group":"IGHV2-70","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-0.855,"Position_3terminus":4,"All_mismatches":"20,18,17,15,14,10,9,8,6,4","Worst_Case_MismatchLocal":"3,3,3,3,3,3,3,3,3,3","Primer_Sequence":"gctccgctgggttttccttg","Terminal_Dinucleotide":"tg","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":20,"gc_clamp_fw":1,"gc_ratio_fw":0.6,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":58.65,"Self_Dimer_DeltaG":0.406,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":3,"Number_of_mismatches_hexamer":2,"_row":"171_openPrimeR2017"}
{"Primer":"Opt5_13","Template":"IGHV3-11","Group":"IGHV3-11","Number_of_mismatches":2,"primer_efficiency":0.263,"annealing_DeltaG":-9.656,"Position_3terminus":16,"All_mismatches":"17,16","Worst_Case_MismatchLocal":"0,0","Primer_Sequence":"gctccgctgggttttccttg","Terminal_Dinucleotide":"tg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":20,"gc_clamp_fw":1,"gc_ratio_fw":0.6,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":58.65,"Self_Dimer_DeltaG":0.406,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"172_openPrimeR2017"}
{"Primer":"Opt5_13","Template":"IGHV3-13","Group":"IGHV3-13","Number_of_mismatches":2,"primer_efficiency":0.263,"annealing_DeltaG":-9.656,"Position_3terminus":16,"All_mismatches":"17,16","Worst_Case_MismatchLocal":"0,0","Primer_Sequence":"gctccgctgggttttccttg","Terminal_Dinucleotide":"tg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":20,"gc_clamp_fw":1,"gc_ratio_fw":0.6,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":58.65,"Self_Dimer_DeltaG":0.406,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"173_openPrimeR2017"}
{"Primer":"Opt5_13","Template":"IGHV3-15","Group":"IGHV3-15","Number_of_mismatches":3,"primer_efficiency":0.0059,"annealing_DeltaG":-7.026,"Position_3terminus":10,"All_mismatches":"17,16,10","Worst_Case_MismatchLocal":"0,0,0","Primer_Sequence":"gctccgctgggttttccttg","Terminal_Dinucleotide":"tg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":20,"gc_clamp_fw":1,"gc_ratio_fw":0.6,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":58.65,"Self_Dimer_DeltaG":0.406,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"174_openPrimeR2017"}
{"Primer":"Opt5_13","Template":"IGHV3-20","Group":"IGHV3-20","Number_of_mismatches":2,"primer_efficiency":0.263,"annealing_DeltaG":-9.656,"Position_3terminus":16,"All_mismatches":"17,16","Worst_Case_MismatchLocal":"0,0","Primer_Sequence":"gctccgctgggttttccttg","Terminal_Dinucleotide":"tg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":20,"gc_clamp_fw":1,"gc_ratio_fw":0.6,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":58.65,"Self_Dimer_DeltaG":0.406,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"175_openPrimeR2017"}
{"Primer":"Opt5_13","Template":"IGHV3-21","Group":"IGHV3-21","Number_of_mismatches":0,"primer_efficiency":0.99893,"annealing_DeltaG":-15.211,"All_mismatches":"NA","Worst_Case_MismatchLocal":"0","Primer_Sequence":"gctccgctgggttttccttg","Terminal_Dinucleotide":"tg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":20,"gc_clamp_fw":1,"gc_ratio_fw":0.6,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":58.65,"Self_Dimer_DeltaG":0.406,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"176_openPrimeR2017"}
{"Primer":"Opt5_13","Template":"IGHV3-23","Group":"IGHV3-23","Number_of_mismatches":4,"primer_efficiency":0.00035,"annealing_DeltaG":-4.878,"Position_3terminus":5,"All_mismatches":"17,16,10,5","Worst_Case_MismatchLocal":"2,2,2,2","Primer_Sequence":"gctccgctgggttttccttg","Terminal_Dinucleotide":"tg","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Amplified","primer_length_fw":20,"gc_clamp_fw":1,"gc_ratio_fw":0.6,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":58.65,"Self_Dimer_DeltaG":0.406,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":2,"Number_of_mismatches_hexamer":1,"_row":"177_openPrimeR2017"}
{"Primer":"Opt5_13","Template":"IGHV3-30","Group":"IGHV3-30","Number_of_mismatches":3,"primer_efficiency":0.06925,"annealing_DeltaG":-9.366,"Position_3terminus":2,"All_mismatches":"17,16,2","Worst_Case_MismatchLocal":"5,5,5","Primer_Sequence":"gctccgctgggttttccttg","Terminal_Dinucleotide":"tg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Amplified","primer_length_fw":20,"gc_clamp_fw":1,"gc_ratio_fw":0.6,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":58.65,"Self_Dimer_DeltaG":0.406,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":1,"_row":"178_openPrimeR2017"}
{"Primer":"Opt5_13","Template":"IGHV3-30-3","Group":"IGHV3-30-3","Number_of_mismatches":3,"primer_efficiency":0.06925,"annealing_DeltaG":-9.366,"Position_3terminus":2,"All_mismatches":"17,16,2","Worst_Case_MismatchLocal":"5,5,5","Primer_Sequence":"gctccgctgggttttccttg","Terminal_Dinucleotide":"tg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Amplified","primer_length_fw":20,"gc_clamp_fw":1,"gc_ratio_fw":0.6,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":58.65,"Self_Dimer_DeltaG":0.406,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":1,"_row":"179_openPrimeR2017"}
{"Primer":"Opt5_13","Template":"IGHV3-33","Group":"IGHV3-33","Number_of_mismatches":3,"primer_efficiency":0.06925,"annealing_DeltaG":-9.366,"Position_3terminus":2,"All_mismatches":"17,16,2","Worst_Case_MismatchLocal":"5,5,5","Primer_Sequence":"gctccgctgggttttccttg","Terminal_Dinucleotide":"tg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Amplified","primer_length_fw":20,"gc_clamp_fw":1,"gc_ratio_fw":0.6,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":58.65,"Self_Dimer_DeltaG":0.406,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":1,"_row":"180_openPrimeR2017"}
{"Primer":"Opt5_13","Template":"IGHV3-43","Group":"IGHV3-43","Number_of_mismatches":3,"primer_efficiency":0.263,"annealing_DeltaG":-9.656,"Position_3terminus":16,"All_mismatches":"20,17,16","Worst_Case_MismatchLocal":"0,0,0","Primer_Sequence":"gctccgctgggttttccttg","Terminal_Dinucleotide":"tg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":20,"gc_clamp_fw":1,"gc_ratio_fw":0.6,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":58.65,"Self_Dimer_DeltaG":0.406,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"181_openPrimeR2017"}
{"Primer":"Opt5_13","Template":"IGHV3-48","Group":"IGHV3-48","Number_of_mismatches":2,"primer_efficiency":0.35271,"annealing_DeltaG":-9.932,"Position_3terminus":16,"All_mismatches":"17,16","Worst_Case_MismatchLocal":"0,0","Primer_Sequence":"gctccgctgggttttccttg","Terminal_Dinucleotide":"tg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":20,"gc_clamp_fw":1,"gc_ratio_fw":0.6,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":58.65,"Self_Dimer_DeltaG":0.406,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"182_openPrimeR2017"}
{"Primer":"Opt5_13","Template":"IGHV3-49","Group":"IGHV3-49","Number_of_mismatches":2,"primer_efficiency":0.263,"annealing_DeltaG":-9.656,"Position_3terminus":16,"All_mismatches":"17,16","Worst_Case_MismatchLocal":"0,0","Primer_Sequence":"gctccgctgggttttccttg","Terminal_Dinucleotide":"tg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":20,"gc_clamp_fw":1,"gc_ratio_fw":0.6,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":58.65,"Self_Dimer_DeltaG":0.406,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"183_openPrimeR2017"}
{"Primer":"Opt5_13","Template":"IGHV3-53","Group":"IGHV3-53","Number_of_mismatches":2,"primer_efficiency":0.263,"annealing_DeltaG":-9.656,"Position_3terminus":16,"All_mismatches":"17,16","Worst_Case_MismatchLocal":"0,0","Primer_Sequence":"gctccgctgggttttccttg","Terminal_Dinucleotide":"tg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":20,"gc_clamp_fw":1,"gc_ratio_fw":0.6,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":58.65,"Self_Dimer_DeltaG":0.406,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"184_openPrimeR2017"}
{"Primer":"Opt5_13","Template":"IGHV3-64","Group":"IGHV3-64","Number_of_mismatches":2,"primer_efficiency":0.263,"annealing_DeltaG":-9.656,"Position_3terminus":16,"All_mismatches":"17,16","Worst_Case_MismatchLocal":"0,0","Primer_Sequence":"gctccgctgggttttccttg","Terminal_Dinucleotide":"tg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":20,"gc_clamp_fw":1,"gc_ratio_fw":0.6,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":58.65,"Self_Dimer_DeltaG":0.406,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"185_openPrimeR2017"}
{"Primer":"Opt5_13","Template":"IGHV3-64D","Group":"IGHV3-64D","Number_of_mismatches":3,"primer_efficiency":0.00998,"annealing_DeltaG":-7.33,"Position_3terminus":7,"All_mismatches":"17,16,7","Worst_Case_MismatchLocal":"0,0,0","Primer_Sequence":"gctccgctgggttttccttg","Terminal_Dinucleotide":"tg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":20,"gc_clamp_fw":1,"gc_ratio_fw":0.6,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":58.65,"Self_Dimer_DeltaG":0.406,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"186_openPrimeR2017"}
{"Primer":"Opt5_13","Template":"IGHV3-66","Group":"IGHV3-66","Number_of_mismatches":2,"primer_efficiency":0.263,"annealing_DeltaG":-9.656,"Position_3terminus":16,"All_mismatches":"17,16","Worst_Case_MismatchLocal":"0,0","Primer_Sequence":"gctccgctgggttttccttg","Terminal_Dinucleotide":"tg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":20,"gc_clamp_fw":1,"gc_ratio_fw":0.6,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":58.65,"Self_Dimer_DeltaG":0.406,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"187_openPrimeR2017"}
{"Primer":"Opt5_13","Template":"IGHV3-7","Group":"IGHV3-7","Number_of_mismatches":2,"primer_efficiency":0.263,"annealing_DeltaG":-9.656,"Position_3terminus":16,"All_mismatches":"17,16","Worst_Case_MismatchLocal":"0,0","Primer_Sequence":"gctccgctgggttttccttg","Terminal_Dinucleotide":"tg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":20,"gc_clamp_fw":1,"gc_ratio_fw":0.6,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":58.65,"Self_Dimer_DeltaG":0.406,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"188_openPrimeR2017"}
{"Primer":"Opt5_13","Template":"IGHV3-72","Group":"IGHV3-72","Number_of_mismatches":2,"primer_efficiency":0.263,"annealing_DeltaG":-9.656,"Position_3terminus":16,"All_mismatches":"17,16","Worst_Case_MismatchLocal":"0,0","Primer_Sequence":"gctccgctgggttttccttg","Terminal_Dinucleotide":"tg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":20,"gc_clamp_fw":1,"gc_ratio_fw":0.6,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":58.65,"Self_Dimer_DeltaG":0.406,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"189_openPrimeR2017"}
{"Primer":"Opt5_13","Template":"IGHV3-73","Group":"IGHV3-73","Number_of_mismatches":2,"primer_efficiency":0.263,"annealing_DeltaG":-9.656,"Position_3terminus":16,"All_mismatches":"17,16","Worst_Case_MismatchLocal":"0,0","Primer_Sequence":"gctccgctgggttttccttg","Terminal_Dinucleotide":"tg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":20,"gc_clamp_fw":1,"gc_ratio_fw":0.6,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":58.65,"Self_Dimer_DeltaG":0.406,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"190_openPrimeR2017"}
{"Primer":"Opt5_13","Template":"IGHV3-74","Group":"IGHV3-74","Number_of_mismatches":2,"primer_efficiency":0.263,"annealing_DeltaG":-9.656,"Position_3terminus":16,"All_mismatches":"17,16","Worst_Case_MismatchLocal":"0,0","Primer_Sequence":"gctccgctgggttttccttg","Terminal_Dinucleotide":"tg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":20,"gc_clamp_fw":1,"gc_ratio_fw":0.6,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":58.65,"Self_Dimer_DeltaG":0.406,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"191_openPrimeR2017"}
{"Primer":"Opt5_13","Template":"IGHV3-9","Group":"IGHV3-9","Number_of_mismatches":5,"primer_efficiency":0.00003,"annealing_DeltaG":-7.047,"Position_3terminus":1,"All_mismatches":"20,17,16,10,1","Worst_Case_MismatchLocal":"6,6,6,6,6","Primer_Sequence":"gctccgctgggttttccttg","Terminal_Dinucleotide":"tg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Unamplified","primer_length_fw":20,"gc_clamp_fw":1,"gc_ratio_fw":0.6,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":58.65,"Self_Dimer_DeltaG":0.406,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":1,"_row":"192_openPrimeR2017"}
{"Primer":"Opt5_13","Template":"IGHV4-28","Group":"IGHV4-28","Number_of_mismatches":8,"primer_efficiency":0,"annealing_DeltaG":-2.539,"Position_3terminus":1,"All_mismatches":"20,18,15,14,10,8,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6","Primer_Sequence":"gctccgctgggttttccttg","Terminal_Dinucleotide":"tg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":20,"gc_clamp_fw":1,"gc_ratio_fw":0.6,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":58.65,"Self_Dimer_DeltaG":0.406,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":2,"_row":"193_openPrimeR2017"}
{"Primer":"Opt5_13","Template":"IGHV4-30-2","Group":"IGHV4-30-2","Number_of_mismatches":8,"primer_efficiency":0,"annealing_DeltaG":-2.539,"Position_3terminus":1,"All_mismatches":"20,18,15,14,10,8,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6","Primer_Sequence":"gctccgctgggttttccttg","Terminal_Dinucleotide":"tg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":20,"gc_clamp_fw":1,"gc_ratio_fw":0.6,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":58.65,"Self_Dimer_DeltaG":0.406,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":2,"_row":"194_openPrimeR2017"}
{"Primer":"Opt5_13","Template":"IGHV4-31","Group":"IGHV4-31","Number_of_mismatches":8,"primer_efficiency":0,"annealing_DeltaG":-1.682,"Position_3terminus":1,"All_mismatches":"16,15,14,9,8,7,5,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6","Primer_Sequence":"gctccgctgggttttccttg","Terminal_Dinucleotide":"tg","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":20,"gc_clamp_fw":1,"gc_ratio_fw":0.6,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":58.65,"Self_Dimer_DeltaG":0.406,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":2,"_row":"195_openPrimeR2017"}
{"Primer":"Opt5_13","Template":"IGHV4-34","Group":"IGHV4-34","Number_of_mismatches":8,"primer_efficiency":0,"annealing_DeltaG":-2.539,"Position_3terminus":1,"All_mismatches":"20,18,15,14,10,8,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6","Primer_Sequence":"gctccgctgggttttccttg","Terminal_Dinucleotide":"tg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":20,"gc_clamp_fw":1,"gc_ratio_fw":0.6,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":58.65,"Self_Dimer_DeltaG":0.406,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":2,"_row":"196_openPrimeR2017"}
{"Primer":"Opt5_13","Template":"IGHV4-38-2","Group":"IGHV4-38-2","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-5.488,"Position_3terminus":1,"All_mismatches":"18,15,14,10,6,4,3,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6","Primer_Sequence":"gctccgctgggttttccttg","Terminal_Dinucleotide":"tg","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":5,"Experimental_Coverage":"Unamplified","primer_length_fw":20,"gc_clamp_fw":1,"gc_ratio_fw":0.6,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":58.65,"Self_Dimer_DeltaG":0.406,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":5,"_row":"197_openPrimeR2017"}
{"Primer":"Opt5_13","Template":"IGHV4-39","Group":"IGHV4-39","Number_of_mismatches":7,"primer_efficiency":0,"annealing_DeltaG":-2.539,"Position_3terminus":1,"All_mismatches":"18,15,14,10,8,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6","Primer_Sequence":"gctccgctgggttttccttg","Terminal_Dinucleotide":"tg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":20,"gc_clamp_fw":1,"gc_ratio_fw":0.6,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":58.65,"Self_Dimer_DeltaG":0.406,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":2,"_row":"198_openPrimeR2017"}
{"Primer":"Opt5_13","Template":"IGHV4-4","Group":"IGHV4-4","Number_of_mismatches":8,"primer_efficiency":0,"annealing_DeltaG":-2.539,"Position_3terminus":1,"All_mismatches":"20,18,15,14,10,8,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6","Primer_Sequence":"gctccgctgggttttccttg","Terminal_Dinucleotide":"tg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":20,"gc_clamp_fw":1,"gc_ratio_fw":0.6,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":58.65,"Self_Dimer_DeltaG":0.406,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":2,"_row":"199_openPrimeR2017"}
{"Primer":"Opt5_13","Template":"IGHV4-59","Group":"IGHV4-59","Number_of_mismatches":8,"primer_efficiency":1.68464e-08,"annealing_DeltaG":-2.891,"Position_3terminus":1,"All_mismatches":"20,18,17,15,14,10,8,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6","Primer_Sequence":"gctccgctgggttttccttg","Terminal_Dinucleotide":"tg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Unamplified","primer_length_fw":20,"gc_clamp_fw":1,"gc_ratio_fw":0.6,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":58.65,"Self_Dimer_DeltaG":0.406,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":1,"_row":"200_openPrimeR2017"}
{"Primer":"Opt5_13","Template":"IGHV4-61","Group":"IGHV4-61","Number_of_mismatches":8,"primer_efficiency":0,"annealing_DeltaG":-2.539,"Position_3terminus":1,"All_mismatches":"20,18,15,14,10,8,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6","Primer_Sequence":"gctccgctgggttttccttg","Terminal_Dinucleotide":"tg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":20,"gc_clamp_fw":1,"gc_ratio_fw":0.6,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":58.65,"Self_Dimer_DeltaG":0.406,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":2,"_row":"201_openPrimeR2017"}
{"Primer":"Opt5_13","Template":"IGHV5-10-1","Group":"IGHV5-10-1","Number_of_mismatches":8,"primer_efficiency":0,"annealing_DeltaG":-5.422,"Position_3terminus":1,"All_mismatches":"19,16,8,7,6,3,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6","Primer_Sequence":"gctccgctgggttttccttg","Terminal_Dinucleotide":"tg","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":20,"gc_clamp_fw":1,"gc_ratio_fw":0.6,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":58.65,"Self_Dimer_DeltaG":0.406,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":4,"_row":"202_openPrimeR2017"}
{"Primer":"Opt5_13","Template":"IGHV5-51","Group":"IGHV5-51","Number_of_mismatches":7,"primer_efficiency":0,"annealing_DeltaG":-4.061,"Position_3terminus":2,"All_mismatches":"18,16,9,7,4,3,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5","Primer_Sequence":"gctccgctgggttttccttg","Terminal_Dinucleotide":"tg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":20,"gc_clamp_fw":1,"gc_ratio_fw":0.6,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":58.65,"Self_Dimer_DeltaG":0.406,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":3,"_row":"203_openPrimeR2017"}
{"Primer":"Opt5_13","Template":"IGHV6-1","Group":"IGHV6-1","Number_of_mismatches":7,"primer_efficiency":0,"annealing_DeltaG":-2.465,"Position_3terminus":1,"All_mismatches":"20,15,14,9,7,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6","Primer_Sequence":"gctccgctgggttttccttg","Terminal_Dinucleotide":"tg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":20,"gc_clamp_fw":1,"gc_ratio_fw":0.6,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":58.65,"Self_Dimer_DeltaG":0.406,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":2,"_row":"204_openPrimeR2017"}
{"Primer":"Opt5_13","Template":"IGHV7-4-1","Group":"IGHV7-4-1","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-1.416,"Position_3terminus":2,"All_mismatches":"19,18,17,16,13,11,9,7,4,3,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"gctccgctgggttttccttg","Terminal_Dinucleotide":"tg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":20,"gc_clamp_fw":1,"gc_ratio_fw":0.6,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":58.65,"Self_Dimer_DeltaG":0.406,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":3,"_row":"205_openPrimeR2017"}
{"Primer":"Opt5_14","Template":"IGHV1-18","Group":"IGHV1-18","Number_of_mismatches":8,"primer_efficiency":0,"annealing_DeltaG":-1.525,"Position_3terminus":1,"All_mismatches":"15,13,10,8,5,3,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6","Primer_Sequence":"tggggtcaaccgccatcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":18,"gc_clamp_fw":2,"gc_ratio_fw":0.66667,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":60.17,"Self_Dimer_DeltaG":-0.724,"Structure_deltaG_fw":-1.63,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":4,"_row":"206_openPrimeR2017"}
{"Primer":"Opt5_14","Template":"IGHV1-2","Group":"IGHV1-2","Number_of_mismatches":7,"primer_efficiency":0,"annealing_DeltaG":-1.881,"Position_3terminus":1,"All_mismatches":"15,13,10,8,3,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6","Primer_Sequence":"tggggtcaaccgccatcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":18,"gc_clamp_fw":2,"gc_ratio_fw":0.66667,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":60.17,"Self_Dimer_DeltaG":-0.724,"Structure_deltaG_fw":-1.63,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"207_openPrimeR2017"}
{"Primer":"Opt5_14","Template":"IGHV1-24","Group":"IGHV1-24","Number_of_mismatches":8,"primer_efficiency":0,"annealing_DeltaG":-2.956,"Position_3terminus":2,"All_mismatches":"13,11,9,7,5,4,3,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5","Primer_Sequence":"tggggtcaaccgccatcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":18,"gc_clamp_fw":2,"gc_ratio_fw":0.66667,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":60.17,"Self_Dimer_DeltaG":-0.724,"Structure_deltaG_fw":-1.63,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":4,"_row":"208_openPrimeR2017"}
{"Primer":"Opt5_14","Template":"IGHV1-3","Group":"IGHV1-3","Number_of_mismatches":7,"primer_efficiency":0,"annealing_DeltaG":-2.305,"Position_3terminus":4,"All_mismatches":"18,14,13,11,8,7,4","Worst_Case_MismatchLocal":"3,3,3,3,3,3,3","Primer_Sequence":"tggggtcaaccgccatcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Unamplified","primer_length_fw":18,"gc_clamp_fw":2,"gc_ratio_fw":0.66667,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":60.17,"Self_Dimer_DeltaG":-0.724,"Structure_deltaG_fw":-1.63,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":3,"Number_of_mismatches_hexamer":1,"_row":"209_openPrimeR2017"}
{"Primer":"Opt5_14","Template":"IGHV1-45","Group":"IGHV1-45","Number_of_mismatches":7,"primer_efficiency":0,"annealing_DeltaG":-1.881,"Position_3terminus":1,"All_mismatches":"15,13,10,8,3,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6","Primer_Sequence":"tggggtcaaccgccatcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":18,"gc_clamp_fw":2,"gc_ratio_fw":0.66667,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":60.17,"Self_Dimer_DeltaG":-0.724,"Structure_deltaG_fw":-1.63,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"210_openPrimeR2017"}
{"Primer":"Opt5_14","Template":"IGHV1-46","Group":"IGHV1-46","Number_of_mismatches":6,"primer_efficiency":0,"annealing_DeltaG":-2.305,"Position_3terminus":3,"All_mismatches":"18,14,10,7,6,3","Worst_Case_MismatchLocal":"4,4,4,4,4,4","Primer_Sequence":"tggggtcaaccgccatcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":18,"gc_clamp_fw":2,"gc_ratio_fw":0.66667,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":60.17,"Self_Dimer_DeltaG":-0.724,"Structure_deltaG_fw":-1.63,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":4,"Number_of_mismatches_hexamer":2,"_row":"211_openPrimeR2017"}
{"Primer":"Opt5_14","Template":"IGHV1-58","Group":"IGHV1-58","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-1.806,"Position_3terminus":1,"All_mismatches":"15,13,12,10,8,5,3,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6","Primer_Sequence":"tggggtcaaccgccatcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":18,"gc_clamp_fw":2,"gc_ratio_fw":0.66667,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":60.17,"Self_Dimer_DeltaG":-0.724,"Structure_deltaG_fw":-1.63,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":4,"_row":"212_openPrimeR2017"}
{"Primer":"Opt5_14","Template":"IGHV1-69","Group":"IGHV1-69","Number_of_mismatches":8,"primer_efficiency":0,"annealing_DeltaG":-2.869,"Position_3terminus":1,"All_mismatches":"13,10,8,7,6,5,3,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6","Primer_Sequence":"tggggtcaaccgccatcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":18,"gc_clamp_fw":2,"gc_ratio_fw":0.66667,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":60.17,"Self_Dimer_DeltaG":-0.724,"Structure_deltaG_fw":-1.63,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":4,"_row":"213_openPrimeR2017"}
{"Primer":"Opt5_14","Template":"IGHV1-69-2","Group":"IGHV1-69-2","Number_of_mismatches":7,"primer_efficiency":0,"annealing_DeltaG":-2.799,"Position_3terminus":1,"All_mismatches":"14,13,8,6,4,3,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6","Primer_Sequence":"tggggtcaaccgccatcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":18,"gc_clamp_fw":2,"gc_ratio_fw":0.66667,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":60.17,"Self_Dimer_DeltaG":-0.724,"Structure_deltaG_fw":-1.63,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":4,"_row":"214_openPrimeR2017"}
{"Primer":"Opt5_14","Template":"IGHV1-8","Group":"IGHV1-8","Number_of_mismatches":8,"primer_efficiency":0,"annealing_DeltaG":-2.351,"Position_3terminus":1,"All_mismatches":"18,15,14,8,7,3,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6","Primer_Sequence":"tggggtcaaccgccatcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":18,"gc_clamp_fw":2,"gc_ratio_fw":0.66667,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":60.17,"Self_Dimer_DeltaG":-0.724,"Structure_deltaG_fw":-1.63,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"215_openPrimeR2017"}
{"Primer":"Opt5_14","Template":"IGHV2-26","Group":"IGHV2-26","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-1.576,"Position_3terminus":1,"All_mismatches":"15,14,8,7,6,5,3,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6","Primer_Sequence":"tggggtcaaccgccatcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":5,"Experimental_Coverage":"Unamplified","primer_length_fw":18,"gc_clamp_fw":2,"gc_ratio_fw":0.66667,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":60.17,"Self_Dimer_DeltaG":-0.724,"Structure_deltaG_fw":-1.63,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":5,"_row":"216_openPrimeR2017"}
{"Primer":"Opt5_14","Template":"IGHV2-5","Group":"IGHV2-5","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-1.576,"Position_3terminus":1,"All_mismatches":"15,14,8,7,6,5,3,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6","Primer_Sequence":"tggggtcaaccgccatcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":5,"Experimental_Coverage":"Unamplified","primer_length_fw":18,"gc_clamp_fw":2,"gc_ratio_fw":0.66667,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":60.17,"Self_Dimer_DeltaG":-0.724,"Structure_deltaG_fw":-1.63,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":5,"_row":"217_openPrimeR2017"}
{"Primer":"Opt5_14","Template":"IGHV2-70","Group":"IGHV2-70","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-1.576,"Position_3terminus":1,"All_mismatches":"15,14,8,7,6,5,3,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6","Primer_Sequence":"tggggtcaaccgccatcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":5,"Experimental_Coverage":"Unamplified","primer_length_fw":18,"gc_clamp_fw":2,"gc_ratio_fw":0.66667,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":60.17,"Self_Dimer_DeltaG":-0.724,"Structure_deltaG_fw":-1.63,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":5,"_row":"218_openPrimeR2017"}
{"Primer":"Opt5_14","Template":"IGHV3-11","Group":"IGHV3-11","Number_of_mismatches":8,"primer_efficiency":0,"annealing_DeltaG":-1.656,"Position_3terminus":3,"All_mismatches":"18,15,13,12,11,9,7,3","Worst_Case_MismatchLocal":"4,4,4,4,4,4,4,4","Primer_Sequence":"tggggtcaaccgccatcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Unamplified","primer_length_fw":18,"gc_clamp_fw":2,"gc_ratio_fw":0.66667,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":60.17,"Self_Dimer_DeltaG":-0.724,"Structure_deltaG_fw":-1.63,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":4,"Number_of_mismatches_hexamer":1,"_row":"219_openPrimeR2017"}
{"Primer":"Opt5_14","Template":"IGHV3-13","Group":"IGHV3-13","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-2.152,"Position_3terminus":1,"All_mismatches":"17,16,15,11,10,7,3,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6","Primer_Sequence":"tggggtcaaccgccatcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":18,"gc_clamp_fw":2,"gc_ratio_fw":0.66667,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":60.17,"Self_Dimer_DeltaG":-0.724,"Structure_deltaG_fw":-1.63,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"220_openPrimeR2017"}
{"Primer":"Opt5_14","Template":"IGHV3-15","Group":"IGHV3-15","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-1.576,"Position_3terminus":1,"All_mismatches":"15,14,8,7,6,5,3,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6","Primer_Sequence":"tggggtcaaccgccatcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":5,"Experimental_Coverage":"Unamplified","primer_length_fw":18,"gc_clamp_fw":2,"gc_ratio_fw":0.66667,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":60.17,"Self_Dimer_DeltaG":-0.724,"Structure_deltaG_fw":-1.63,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":5,"_row":"221_openPrimeR2017"}
{"Primer":"Opt5_14","Template":"IGHV3-20","Group":"IGHV3-20","Number_of_mismatches":8,"primer_efficiency":0,"annealing_DeltaG":-5.752,"Position_3terminus":4,"All_mismatches":"18,17,11,10,7,6,5,4","Worst_Case_MismatchLocal":"3,3,3,3,3,3,3,3","Primer_Sequence":"tggggtcaaccgccatcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":18,"gc_clamp_fw":2,"gc_ratio_fw":0.66667,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":60.17,"Self_Dimer_DeltaG":-0.724,"Structure_deltaG_fw":-1.63,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":3,"Number_of_mismatches_hexamer":3,"_row":"222_openPrimeR2017"}
{"Primer":"Opt5_14","Template":"IGHV3-21","Group":"IGHV3-21","Number_of_mismatches":8,"primer_efficiency":0,"annealing_DeltaG":-2.869,"Position_3terminus":1,"All_mismatches":"13,11,10,9,8,4,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6","Primer_Sequence":"tggggtcaaccgccatcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":18,"gc_clamp_fw":2,"gc_ratio_fw":0.66667,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":60.17,"Self_Dimer_DeltaG":-0.724,"Structure_deltaG_fw":-1.63,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"223_openPrimeR2017"}
{"Primer":"Opt5_14","Template":"IGHV3-23","Group":"IGHV3-23","Number_of_mismatches":8,"primer_efficiency":0,"annealing_DeltaG":-1.891,"Position_3terminus":1,"All_mismatches":"14,12,10,8,6,4,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6","Primer_Sequence":"tggggtcaaccgccatcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":18,"gc_clamp_fw":2,"gc_ratio_fw":0.66667,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":60.17,"Self_Dimer_DeltaG":-0.724,"Structure_deltaG_fw":-1.63,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":4,"_row":"224_openPrimeR2017"}
{"Primer":"Opt5_14","Template":"IGHV3-30","Group":"IGHV3-30","Number_of_mismatches":8,"primer_efficiency":0,"annealing_DeltaG":-2.956,"Position_3terminus":2,"All_mismatches":"13,12,10,9,8,4,3,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5","Primer_Sequence":"tggggtcaaccgccatcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":18,"gc_clamp_fw":2,"gc_ratio_fw":0.66667,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":60.17,"Self_Dimer_DeltaG":-0.724,"Structure_deltaG_fw":-1.63,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":3,"_row":"225_openPrimeR2017"}
{"Primer":"Opt5_14","Template":"IGHV3-30-3","Group":"IGHV3-30-3","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-1.576,"Position_3terminus":1,"All_mismatches":"15,14,8,7,6,5,3,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6","Primer_Sequence":"tggggtcaaccgccatcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":5,"Experimental_Coverage":"Unamplified","primer_length_fw":18,"gc_clamp_fw":2,"gc_ratio_fw":0.66667,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":60.17,"Self_Dimer_DeltaG":-0.724,"Structure_deltaG_fw":-1.63,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":5,"_row":"226_openPrimeR2017"}
{"Primer":"Opt5_14","Template":"IGHV3-33","Group":"IGHV3-33","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-1.576,"Position_3terminus":1,"All_mismatches":"15,14,8,7,6,5,3,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6","Primer_Sequence":"tggggtcaaccgccatcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":5,"Experimental_Coverage":"Unamplified","primer_length_fw":18,"gc_clamp_fw":2,"gc_ratio_fw":0.66667,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":60.17,"Self_Dimer_DeltaG":-0.724,"Structure_deltaG_fw":-1.63,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":5,"_row":"227_openPrimeR2017"}
{"Primer":"Opt5_14","Template":"IGHV3-43","Group":"IGHV3-43","Number_of_mismatches":8,"primer_efficiency":0,"annealing_DeltaG":-1.656,"Position_3terminus":3,"All_mismatches":"18,15,13,12,11,9,7,3","Worst_Case_MismatchLocal":"4,4,4,4,4,4,4,4","Primer_Sequence":"tggggtcaaccgccatcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Unamplified","primer_length_fw":18,"gc_clamp_fw":2,"gc_ratio_fw":0.66667,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":60.17,"Self_Dimer_DeltaG":-0.724,"Structure_deltaG_fw":-1.63,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":4,"Number_of_mismatches_hexamer":1,"_row":"228_openPrimeR2017"}
{"Primer":"Opt5_14","Template":"IGHV3-48","Group":"IGHV3-48","Number_of_mismatches":8,"primer_efficiency":0,"annealing_DeltaG":-1.229,"Position_3terminus":2,"All_mismatches":"15,14,11,9,8,5,4,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5","Primer_Sequence":"tggggtcaaccgccatcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":18,"gc_clamp_fw":2,"gc_ratio_fw":0.66667,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":60.17,"Self_Dimer_DeltaG":-0.724,"Structure_deltaG_fw":-1.63,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":3,"_row":"229_openPrimeR2017"}
{"Primer":"Opt5_14","Template":"IGHV3-49","Group":"IGHV3-49","Number_of_mismatches":8,"primer_efficiency":0,"annealing_DeltaG":-1.656,"Position_3terminus":3,"All_mismatches":"18,15,13,12,11,9,7,3","Worst_Case_MismatchLocal":"4,4,4,4,4,4,4,4","Primer_Sequence":"tggggtcaaccgccatcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Unamplified","primer_length_fw":18,"gc_clamp_fw":2,"gc_ratio_fw":0.66667,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":60.17,"Self_Dimer_DeltaG":-0.724,"Structure_deltaG_fw":-1.63,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":4,"Number_of_mismatches_hexamer":1,"_row":"230_openPrimeR2017"}
{"Primer":"Opt5_14","Template":"IGHV3-53","Group":"IGHV3-53","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-1.576,"Position_3terminus":1,"All_mismatches":"15,14,8,7,6,5,3,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6","Primer_Sequence":"tggggtcaaccgccatcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":5,"Experimental_Coverage":"Unamplified","primer_length_fw":18,"gc_clamp_fw":2,"gc_ratio_fw":0.66667,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":60.17,"Self_Dimer_DeltaG":-0.724,"Structure_deltaG_fw":-1.63,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":5,"_row":"231_openPrimeR2017"}
{"Primer":"Opt5_14","Template":"IGHV3-64","Group":"IGHV3-64","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-1.576,"Position_3terminus":1,"All_mismatches":"15,14,8,7,6,5,3,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6","Primer_Sequence":"tggggtcaaccgccatcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":5,"Experimental_Coverage":"Unamplified","primer_length_fw":18,"gc_clamp_fw":2,"gc_ratio_fw":0.66667,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":60.17,"Self_Dimer_DeltaG":-0.724,"Structure_deltaG_fw":-1.63,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":5,"_row":"232_openPrimeR2017"}
{"Primer":"Opt5_14","Template":"IGHV3-64D","Group":"IGHV3-64D","Number_of_mismatches":8,"primer_efficiency":0,"annealing_DeltaG":-2.956,"Position_3terminus":1,"All_mismatches":"13,12,10,9,7,6,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6","Primer_Sequence":"tggggtcaaccgccatcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":18,"gc_clamp_fw":2,"gc_ratio_fw":0.66667,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":60.17,"Self_Dimer_DeltaG":-0.724,"Structure_deltaG_fw":-1.63,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"233_openPrimeR2017"}
{"Primer":"Opt5_14","Template":"IGHV3-66","Group":"IGHV3-66","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-1.576,"Position_3terminus":1,"All_mismatches":"15,14,8,7,6,5,3,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6","Primer_Sequence":"tggggtcaaccgccatcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":5,"Experimental_Coverage":"Unamplified","primer_length_fw":18,"gc_clamp_fw":2,"gc_ratio_fw":0.66667,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":60.17,"Self_Dimer_DeltaG":-0.724,"Structure_deltaG_fw":-1.63,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":5,"_row":"234_openPrimeR2017"}
{"Primer":"Opt5_14","Template":"IGHV3-7","Group":"IGHV3-7","Number_of_mismatches":7,"primer_efficiency":0,"annealing_DeltaG":-1.617,"Position_3terminus":2,"All_mismatches":"15,14,11,9,8,4,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5","Primer_Sequence":"tggggtcaaccgccatcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":18,"gc_clamp_fw":2,"gc_ratio_fw":0.66667,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":60.17,"Self_Dimer_DeltaG":-0.724,"Structure_deltaG_fw":-1.63,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":2,"_row":"235_openPrimeR2017"}
{"Primer":"Opt5_14","Template":"IGHV3-72","Group":"IGHV3-72","Number_of_mismatches":8,"primer_efficiency":0,"annealing_DeltaG":-5.752,"Position_3terminus":4,"All_mismatches":"18,17,11,10,7,6,5,4","Worst_Case_MismatchLocal":"3,3,3,3,3,3,3,3","Primer_Sequence":"tggggtcaaccgccatcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":18,"gc_clamp_fw":2,"gc_ratio_fw":0.66667,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":60.17,"Self_Dimer_DeltaG":-0.724,"Structure_deltaG_fw":-1.63,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":3,"Number_of_mismatches_hexamer":3,"_row":"236_openPrimeR2017"}
{"Primer":"Opt5_14","Template":"IGHV3-73","Group":"IGHV3-73","Number_of_mismatches":7,"primer_efficiency":0,"annealing_DeltaG":-5.752,"Position_3terminus":4,"All_mismatches":"14,13,11,10,8,6,4","Worst_Case_MismatchLocal":"3,3,3,3,3,3,3","Primer_Sequence":"tggggtcaaccgccatcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":18,"gc_clamp_fw":2,"gc_ratio_fw":0.66667,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":60.17,"Self_Dimer_DeltaG":-0.724,"Structure_deltaG_fw":-1.63,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":3,"Number_of_mismatches_hexamer":2,"_row":"237_openPrimeR2017"}
{"Primer":"Opt5_14","Template":"IGHV3-74","Group":"IGHV3-74","Number_of_mismatches":9,"primer_efficiency":0.00001,"annealing_DeltaG":-2.869,"Position_3terminus":3,"All_mismatches":"18,17,16,13,12,11,9,7,3","Worst_Case_MismatchLocal":"4,4,4,4,4,4,4,4,4","Primer_Sequence":"tggggtcaaccgccatcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Unamplified","primer_length_fw":18,"gc_clamp_fw":2,"gc_ratio_fw":0.66667,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":60.17,"Self_Dimer_DeltaG":-0.724,"Structure_deltaG_fw":-1.63,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":4,"Number_of_mismatches_hexamer":1,"_row":"238_openPrimeR2017"}
{"Primer":"Opt5_14","Template":"IGHV3-9","Group":"IGHV3-9","Number_of_mismatches":8,"primer_efficiency":0,"annealing_DeltaG":-2.956,"Position_3terminus":2,"All_mismatches":"13,12,11,10,8,6,5,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5","Primer_Sequence":"tggggtcaaccgccatcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":18,"gc_clamp_fw":2,"gc_ratio_fw":0.66667,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":60.17,"Self_Dimer_DeltaG":-0.724,"Structure_deltaG_fw":-1.63,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":3,"_row":"239_openPrimeR2017"}
{"Primer":"Opt5_14","Template":"IGHV4-28","Group":"IGHV4-28","Number_of_mismatches":8,"primer_efficiency":0,"annealing_DeltaG":-1.525,"Position_3terminus":1,"All_mismatches":"15,13,10,8,7,3,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6","Primer_Sequence":"tggggtcaaccgccatcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":18,"gc_clamp_fw":2,"gc_ratio_fw":0.66667,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":60.17,"Self_Dimer_DeltaG":-0.724,"Structure_deltaG_fw":-1.63,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"240_openPrimeR2017"}
{"Primer":"Opt5_14","Template":"IGHV4-30-2","Group":"IGHV4-30-2","Number_of_mismatches":8,"primer_efficiency":0,"annealing_DeltaG":-1.525,"Position_3terminus":1,"All_mismatches":"15,13,10,8,7,3,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6","Primer_Sequence":"tggggtcaaccgccatcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":18,"gc_clamp_fw":2,"gc_ratio_fw":0.66667,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":60.17,"Self_Dimer_DeltaG":-0.724,"Structure_deltaG_fw":-1.63,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"241_openPrimeR2017"}
{"Primer":"Opt5_14","Template":"IGHV4-31","Group":"IGHV4-31","Number_of_mismatches":8,"primer_efficiency":0,"annealing_DeltaG":-1.525,"Position_3terminus":1,"All_mismatches":"15,13,10,8,7,3,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6","Primer_Sequence":"tggggtcaaccgccatcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":18,"gc_clamp_fw":2,"gc_ratio_fw":0.66667,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":60.17,"Self_Dimer_DeltaG":-0.724,"Structure_deltaG_fw":-1.63,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"242_openPrimeR2017"}
{"Primer":"Opt5_14","Template":"IGHV4-34","Group":"IGHV4-34","Number_of_mismatches":8,"primer_efficiency":7.27302e-08,"annealing_DeltaG":-1.96,"Position_3terminus":4,"All_mismatches":"16,12,11,10,9,7,6,4","Worst_Case_MismatchLocal":"3,3,3,3,3,3,3,3","Primer_Sequence":"tggggtcaaccgccatcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":18,"gc_clamp_fw":2,"gc_ratio_fw":0.66667,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":60.17,"Self_Dimer_DeltaG":-0.724,"Structure_deltaG_fw":-1.63,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":3,"Number_of_mismatches_hexamer":2,"_row":"243_openPrimeR2017"}
{"Primer":"Opt5_14","Template":"IGHV4-38-2","Group":"IGHV4-38-2","Number_of_mismatches":8,"primer_efficiency":0,"annealing_DeltaG":-1.96,"Position_3terminus":1,"All_mismatches":"16,12,11,10,7,4,3,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6","Primer_Sequence":"tggggtcaaccgccatcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":18,"gc_clamp_fw":2,"gc_ratio_fw":0.66667,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":60.17,"Self_Dimer_DeltaG":-0.724,"Structure_deltaG_fw":-1.63,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"244_openPrimeR2017"}
{"Primer":"Opt5_14","Template":"IGHV4-39","Group":"IGHV4-39","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-1.576,"Position_3terminus":1,"All_mismatches":"15,14,8,7,6,5,3,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6","Primer_Sequence":"tggggtcaaccgccatcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":5,"Experimental_Coverage":"Unamplified","primer_length_fw":18,"gc_clamp_fw":2,"gc_ratio_fw":0.66667,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":60.17,"Self_Dimer_DeltaG":-0.724,"Structure_deltaG_fw":-1.63,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":5,"_row":"245_openPrimeR2017"}
{"Primer":"Opt5_14","Template":"IGHV4-4","Group":"IGHV4-4","Number_of_mismatches":8,"primer_efficiency":0,"annealing_DeltaG":-1.525,"Position_3terminus":1,"All_mismatches":"15,13,10,8,7,3,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6","Primer_Sequence":"tggggtcaaccgccatcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":18,"gc_clamp_fw":2,"gc_ratio_fw":0.66667,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":60.17,"Self_Dimer_DeltaG":-0.724,"Structure_deltaG_fw":-1.63,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"246_openPrimeR2017"}
{"Primer":"Opt5_14","Template":"IGHV4-59","Group":"IGHV4-59","Number_of_mismatches":8,"primer_efficiency":0,"annealing_DeltaG":-1.576,"Position_3terminus":1,"All_mismatches":"15,14,8,7,6,5,3,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6","Primer_Sequence":"tggggtcaaccgccatcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":18,"gc_clamp_fw":2,"gc_ratio_fw":0.66667,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":60.17,"Self_Dimer_DeltaG":-0.724,"Structure_deltaG_fw":-1.63,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":4,"_row":"247_openPrimeR2017"}
{"Primer":"Opt5_14","Template":"IGHV4-61","Group":"IGHV4-61","Number_of_mismatches":8,"primer_efficiency":7.27302e-08,"annealing_DeltaG":-1.96,"Position_3terminus":4,"All_mismatches":"16,12,11,10,9,7,6,4","Worst_Case_MismatchLocal":"3,3,3,3,3,3,3,3","Primer_Sequence":"tggggtcaaccgccatcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":18,"gc_clamp_fw":2,"gc_ratio_fw":0.66667,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":60.17,"Self_Dimer_DeltaG":-0.724,"Structure_deltaG_fw":-1.63,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":3,"Number_of_mismatches_hexamer":2,"_row":"248_openPrimeR2017"}
{"Primer":"Opt5_14","Template":"IGHV5-10-1","Group":"IGHV5-10-1","Number_of_mismatches":0,"primer_efficiency":0.9993,"annealing_DeltaG":-14.955,"All_mismatches":"NA","Worst_Case_MismatchLocal":"0","Primer_Sequence":"tggggtcaaccgccatcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":18,"gc_clamp_fw":2,"gc_ratio_fw":0.66667,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":60.17,"Self_Dimer_DeltaG":-0.724,"Structure_deltaG_fw":-1.63,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"249_openPrimeR2017"}
{"Primer":"Opt5_14","Template":"IGHV5-51","Group":"IGHV5-51","Number_of_mismatches":0,"primer_efficiency":0.9993,"annealing_DeltaG":-14.955,"All_mismatches":"NA","Worst_Case_MismatchLocal":"0","Primer_Sequence":"tggggtcaaccgccatcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":18,"gc_clamp_fw":2,"gc_ratio_fw":0.66667,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":60.17,"Self_Dimer_DeltaG":-0.724,"Structure_deltaG_fw":-1.63,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"250_openPrimeR2017"}
{"Primer":"Opt5_14","Template":"IGHV6-1","Group":"IGHV6-1","Number_of_mismatches":8,"primer_efficiency":0,"annealing_DeltaG":-1.27,"Position_3terminus":4,"All_mismatches":"18,16,15,10,8,6,5,4","Worst_Case_MismatchLocal":"3,3,3,3,3,3,3,3","Primer_Sequence":"tggggtcaaccgccatcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":18,"gc_clamp_fw":2,"gc_ratio_fw":0.66667,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":60.17,"Self_Dimer_DeltaG":-0.724,"Structure_deltaG_fw":-1.63,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":3,"Number_of_mismatches_hexamer":3,"_row":"251_openPrimeR2017"}
{"Primer":"Opt5_14","Template":"IGHV7-4-1","Group":"IGHV7-4-1","Number_of_mismatches":6,"primer_efficiency":0,"annealing_DeltaG":-3.323,"Position_3terminus":3,"All_mismatches":"18,14,10,7,6,3","Worst_Case_MismatchLocal":"4,4,4,4,4,4","Primer_Sequence":"tggggtcaaccgccatcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":18,"gc_clamp_fw":2,"gc_ratio_fw":0.66667,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":60.17,"Self_Dimer_DeltaG":-0.724,"Structure_deltaG_fw":-1.63,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":4,"Number_of_mismatches_hexamer":2,"_row":"252_openPrimeR2017"}
{"Primer":"Opt5_15","Template":"IGHV1-18","Group":"IGHV1-18","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-2.462,"Position_3terminus":1,"All_mismatches":"20,19,18,17,13,10,9,7,5,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"ggcctctccacttaaacccagg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":3,"no_repeats_fw":2,"melting_temp":59.41,"Self_Dimer_DeltaG":-1.915,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":2,"_row":"253_openPrimeR2017"}
{"Primer":"Opt5_15","Template":"IGHV1-2","Group":"IGHV1-2","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-4.191,"Position_3terminus":1,"All_mismatches":"22,19,16,13,12,11,10,7,5,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"ggcctctccacttaaacccagg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":3,"no_repeats_fw":2,"melting_temp":59.41,"Self_Dimer_DeltaG":-1.915,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"254_openPrimeR2017"}
{"Primer":"Opt5_15","Template":"IGHV1-24","Group":"IGHV1-24","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-3.105,"Position_3terminus":1,"All_mismatches":"22,21,18,15,12,11,10,9,8,7,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"ggcctctccacttaaacccagg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":3,"no_repeats_fw":2,"melting_temp":59.41,"Self_Dimer_DeltaG":-1.915,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":1,"_row":"255_openPrimeR2017"}
{"Primer":"Opt5_15","Template":"IGHV1-3","Group":"IGHV1-3","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-2.495,"Position_3terminus":1,"All_mismatches":"22,21,20,18,13,11,10,7,3,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"ggcctctccacttaaacccagg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":3,"no_repeats_fw":2,"melting_temp":59.41,"Self_Dimer_DeltaG":-1.915,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"256_openPrimeR2017"}
{"Primer":"Opt5_15","Template":"IGHV1-45","Group":"IGHV1-45","Number_of_mismatches":12,"primer_efficiency":0,"annealing_DeltaG":-3.272,"Position_3terminus":1,"All_mismatches":"21,20,19,18,14,13,9,8,7,6,5,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"ggcctctccacttaaacccagg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":3,"no_repeats_fw":2,"melting_temp":59.41,"Self_Dimer_DeltaG":-1.915,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"257_openPrimeR2017"}
{"Primer":"Opt5_15","Template":"IGHV1-46","Group":"IGHV1-46","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-4.09,"Position_3terminus":2,"All_mismatches":"22,21,19,14,11,10,7,4,3,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"ggcctctccacttaaacccagg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":3,"no_repeats_fw":2,"melting_temp":59.41,"Self_Dimer_DeltaG":-1.915,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":3,"_row":"258_openPrimeR2017"}
{"Primer":"Opt5_15","Template":"IGHV1-58","Group":"IGHV1-58","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-2.912,"Position_3terminus":1,"All_mismatches":"20,19,18,16,15,13,11,10,6,5,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"ggcctctccacttaaacccagg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":3,"no_repeats_fw":2,"melting_temp":59.41,"Self_Dimer_DeltaG":-1.915,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"259_openPrimeR2017"}
{"Primer":"Opt5_15","Template":"IGHV1-69","Group":"IGHV1-69","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-1.615,"Position_3terminus":1,"All_mismatches":"19,16,14,13,12,11,10,7,5,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"ggcctctccacttaaacccagg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":3,"no_repeats_fw":2,"melting_temp":59.41,"Self_Dimer_DeltaG":-1.915,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"260_openPrimeR2017"}
{"Primer":"Opt5_15","Template":"IGHV1-69-2","Group":"IGHV1-69-2","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-3.272,"Position_3terminus":1,"All_mismatches":"20,19,18,13,9,8,7,6,5,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"ggcctctccacttaaacccagg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":3,"no_repeats_fw":2,"melting_temp":59.41,"Self_Dimer_DeltaG":-1.915,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"261_openPrimeR2017"}
{"Primer":"Opt5_15","Template":"IGHV1-8","Group":"IGHV1-8","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-3.505,"Position_3terminus":2,"All_mismatches":"21,15,14,12,11,10,7,4,3,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"ggcctctccacttaaacccagg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":3,"no_repeats_fw":2,"melting_temp":59.41,"Self_Dimer_DeltaG":-1.915,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":3,"_row":"262_openPrimeR2017"}
{"Primer":"Opt5_15","Template":"IGHV2-26","Group":"IGHV2-26","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-2.61,"Position_3terminus":1,"All_mismatches":"22,18,16,12,11,10,8,5,3,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"ggcctctccacttaaacccagg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":3,"no_repeats_fw":2,"melting_temp":59.41,"Self_Dimer_DeltaG":-1.915,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":4,"_row":"263_openPrimeR2017"}
{"Primer":"Opt5_15","Template":"IGHV2-5","Group":"IGHV2-5","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-2.61,"Position_3terminus":1,"All_mismatches":"22,18,16,12,11,10,8,5,3,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"ggcctctccacttaaacccagg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":3,"no_repeats_fw":2,"melting_temp":59.41,"Self_Dimer_DeltaG":-1.915,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":4,"_row":"264_openPrimeR2017"}
{"Primer":"Opt5_15","Template":"IGHV2-70","Group":"IGHV2-70","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-3.678,"Position_3terminus":1,"All_mismatches":"22,21,19,18,16,14,13,12,10,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"ggcctctccacttaaacccagg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":3,"no_repeats_fw":2,"melting_temp":59.41,"Self_Dimer_DeltaG":-1.915,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":2,"_row":"265_openPrimeR2017"}
{"Primer":"Opt5_15","Template":"IGHV3-11","Group":"IGHV3-11","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-3.44,"Position_3terminus":2,"All_mismatches":"21,18,17,16,13,11,10,7,6,3,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"ggcctctccacttaaacccagg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":3,"no_repeats_fw":2,"melting_temp":59.41,"Self_Dimer_DeltaG":-1.915,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":3,"_row":"266_openPrimeR2017"}
{"Primer":"Opt5_15","Template":"IGHV3-13","Group":"IGHV3-13","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-4.09,"Position_3terminus":2,"All_mismatches":"22,20,13,12,10,9,7,4,3,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"ggcctctccacttaaacccagg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":3,"no_repeats_fw":2,"melting_temp":59.41,"Self_Dimer_DeltaG":-1.915,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":3,"_row":"267_openPrimeR2017"}
{"Primer":"Opt5_15","Template":"IGHV3-15","Group":"IGHV3-15","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-4.09,"Position_3terminus":2,"All_mismatches":"22,20,13,12,10,9,7,4,3,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"ggcctctccacttaaacccagg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":3,"no_repeats_fw":2,"melting_temp":59.41,"Self_Dimer_DeltaG":-1.915,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":3,"_row":"268_openPrimeR2017"}
{"Primer":"Opt5_15","Template":"IGHV3-20","Group":"IGHV3-20","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-4.09,"Position_3terminus":2,"All_mismatches":"22,20,13,12,10,9,7,4,3,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"ggcctctccacttaaacccagg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":3,"no_repeats_fw":2,"melting_temp":59.41,"Self_Dimer_DeltaG":-1.915,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":3,"_row":"269_openPrimeR2017"}
{"Primer":"Opt5_15","Template":"IGHV3-21","Group":"IGHV3-21","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-3.646,"Position_3terminus":2,"All_mismatches":"17,16,15,13,10,9,7,6,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5","Primer_Sequence":"ggcctctccacttaaacccagg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":3,"no_repeats_fw":2,"melting_temp":59.41,"Self_Dimer_DeltaG":-1.915,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":2,"_row":"270_openPrimeR2017"}
{"Primer":"Opt5_15","Template":"IGHV3-23","Group":"IGHV3-23","Number_of_mismatches":12,"primer_efficiency":0,"annealing_DeltaG":-0.194,"Position_3terminus":3,"All_mismatches":"20,19,16,15,13,11,10,8,7,6,4,3","Worst_Case_MismatchLocal":"4,4,4,4,4,4,4,4,4,4,4,4","Primer_Sequence":"ggcctctccacttaaacccagg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":3,"no_repeats_fw":2,"melting_temp":59.41,"Self_Dimer_DeltaG":-1.915,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":4,"Number_of_mismatches_hexamer":3,"_row":"271_openPrimeR2017"}
{"Primer":"Opt5_15","Template":"IGHV3-30","Group":"IGHV3-30","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-3.183,"Position_3terminus":1,"All_mismatches":"21,20,18,16,15,10,9,6,5,3,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"ggcctctccacttaaacccagg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":3,"no_repeats_fw":2,"melting_temp":59.41,"Self_Dimer_DeltaG":-1.915,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":4,"_row":"272_openPrimeR2017"}
{"Primer":"Opt5_15","Template":"IGHV3-30-3","Group":"IGHV3-30-3","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-2.051,"Position_3terminus":2,"All_mismatches":"21,20,19,16,15,10,7,6,4,3,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"ggcctctccacttaaacccagg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":3,"no_repeats_fw":2,"melting_temp":59.41,"Self_Dimer_DeltaG":-1.915,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":4,"_row":"273_openPrimeR2017"}
{"Primer":"Opt5_15","Template":"IGHV3-33","Group":"IGHV3-33","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-1.431,"Position_3terminus":2,"All_mismatches":"21,20,19,16,15,10,7,6,4,3,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"ggcctctccacttaaacccagg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":3,"no_repeats_fw":2,"melting_temp":59.41,"Self_Dimer_DeltaG":-1.915,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":4,"_row":"274_openPrimeR2017"}
{"Primer":"Opt5_15","Template":"IGHV3-43","Group":"IGHV3-43","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-4.579,"Position_3terminus":7,"All_mismatches":"22,21,18,17,16,13,12,11,10,8,7","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0,0,0,0,0","Primer_Sequence":"ggcctctccacttaaacccagg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":3,"no_repeats_fw":2,"melting_temp":59.41,"Self_Dimer_DeltaG":-1.915,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"275_openPrimeR2017"}
{"Primer":"Opt5_15","Template":"IGHV3-48","Group":"IGHV3-48","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-3.055,"Position_3terminus":2,"All_mismatches":"22,17,16,15,13,10,9,7,6,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"ggcctctccacttaaacccagg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":3,"no_repeats_fw":2,"melting_temp":59.41,"Self_Dimer_DeltaG":-1.915,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":2,"_row":"276_openPrimeR2017"}
{"Primer":"Opt5_15","Template":"IGHV3-49","Group":"IGHV3-49","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-3.795,"Position_3terminus":2,"All_mismatches":"20,19,17,9,8,7,6,5,4,3,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"ggcctctccacttaaacccagg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":5,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":3,"no_repeats_fw":2,"melting_temp":59.41,"Self_Dimer_DeltaG":-1.915,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":5,"_row":"277_openPrimeR2017"}
{"Primer":"Opt5_15","Template":"IGHV3-53","Group":"IGHV3-53","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-1.431,"Position_3terminus":2,"All_mismatches":"21,20,19,16,15,10,7,6,4,3,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"ggcctctccacttaaacccagg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":3,"no_repeats_fw":2,"melting_temp":59.41,"Self_Dimer_DeltaG":-1.915,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":4,"_row":"278_openPrimeR2017"}
{"Primer":"Opt5_15","Template":"IGHV3-64","Group":"IGHV3-64","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-4.579,"Position_3terminus":7,"All_mismatches":"22,21,18,17,16,13,12,11,10,8,7","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0,0,0,0,0","Primer_Sequence":"ggcctctccacttaaacccagg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":3,"no_repeats_fw":2,"melting_temp":59.41,"Self_Dimer_DeltaG":-1.915,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"279_openPrimeR2017"}
{"Primer":"Opt5_15","Template":"IGHV3-64D","Group":"IGHV3-64D","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-4.09,"Position_3terminus":2,"All_mismatches":"22,20,13,12,9,7,4,3,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5","Primer_Sequence":"ggcctctccacttaaacccagg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":3,"no_repeats_fw":2,"melting_temp":59.41,"Self_Dimer_DeltaG":-1.915,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":3,"_row":"280_openPrimeR2017"}
{"Primer":"Opt5_15","Template":"IGHV3-66","Group":"IGHV3-66","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-1.431,"Position_3terminus":2,"All_mismatches":"21,20,19,15,10,7,6,4,3,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"ggcctctccacttaaacccagg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":3,"no_repeats_fw":2,"melting_temp":59.41,"Self_Dimer_DeltaG":-1.915,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":4,"_row":"281_openPrimeR2017"}
{"Primer":"Opt5_15","Template":"IGHV3-7","Group":"IGHV3-7","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-6.115,"Position_3terminus":2,"All_mismatches":"17,9,8,7,6,5,4,3,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5","Primer_Sequence":"ggcctctccacttaaacccagg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":5,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":3,"no_repeats_fw":2,"melting_temp":59.41,"Self_Dimer_DeltaG":-1.915,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":5,"_row":"282_openPrimeR2017"}
{"Primer":"Opt5_15","Template":"IGHV3-72","Group":"IGHV3-72","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-4.09,"Position_3terminus":2,"All_mismatches":"22,20,13,12,10,9,7,4,3,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"ggcctctccacttaaacccagg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":3,"no_repeats_fw":2,"melting_temp":59.41,"Self_Dimer_DeltaG":-1.915,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":3,"_row":"283_openPrimeR2017"}
{"Primer":"Opt5_15","Template":"IGHV3-73","Group":"IGHV3-73","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-4.09,"Position_3terminus":2,"All_mismatches":"22,21,20,13,12,10,9,7,4,3,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"ggcctctccacttaaacccagg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":3,"no_repeats_fw":2,"melting_temp":59.41,"Self_Dimer_DeltaG":-1.915,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":3,"_row":"284_openPrimeR2017"}
{"Primer":"Opt5_15","Template":"IGHV3-74","Group":"IGHV3-74","Number_of_mismatches":12,"primer_efficiency":0,"annealing_DeltaG":-3.802,"Position_3terminus":1,"All_mismatches":"22,21,18,17,16,13,12,11,10,8,7,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"ggcctctccacttaaacccagg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":3,"no_repeats_fw":2,"melting_temp":59.41,"Self_Dimer_DeltaG":-1.915,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":1,"_row":"285_openPrimeR2017"}
{"Primer":"Opt5_15","Template":"IGHV3-9","Group":"IGHV3-9","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-4.579,"Position_3terminus":7,"All_mismatches":"20,19,18,17,16,13,12,11,10,8,7","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0,0,0,0,0","Primer_Sequence":"ggcctctccacttaaacccagg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":3,"no_repeats_fw":2,"melting_temp":59.41,"Self_Dimer_DeltaG":-1.915,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"286_openPrimeR2017"}
{"Primer":"Opt5_15","Template":"IGHV4-28","Group":"IGHV4-28","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-3.96,"Position_3terminus":7,"All_mismatches":"22,19,18,16,15,14,12,10,9,8,7","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0,0,0,0,0","Primer_Sequence":"ggcctctccacttaaacccagg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":3,"no_repeats_fw":2,"melting_temp":59.41,"Self_Dimer_DeltaG":-1.915,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"287_openPrimeR2017"}
{"Primer":"Opt5_15","Template":"IGHV4-30-2","Group":"IGHV4-30-2","Number_of_mismatches":12,"primer_efficiency":0,"annealing_DeltaG":-0.194,"Position_3terminus":3,"All_mismatches":"20,19,16,15,13,11,10,8,7,6,4,3","Worst_Case_MismatchLocal":"4,4,4,4,4,4,4,4,4,4,4,4","Primer_Sequence":"ggcctctccacttaaacccagg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":3,"no_repeats_fw":2,"melting_temp":59.41,"Self_Dimer_DeltaG":-1.915,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":4,"Number_of_mismatches_hexamer":3,"_row":"288_openPrimeR2017"}
{"Primer":"Opt5_15","Template":"IGHV4-31","Group":"IGHV4-31","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-3.96,"Position_3terminus":7,"All_mismatches":"22,19,18,16,15,14,12,10,9,8,7","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0,0,0,0,0","Primer_Sequence":"ggcctctccacttaaacccagg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":3,"no_repeats_fw":2,"melting_temp":59.41,"Self_Dimer_DeltaG":-1.915,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"289_openPrimeR2017"}
{"Primer":"Opt5_15","Template":"IGHV4-34","Group":"IGHV4-34","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-1.056,"Position_3terminus":5,"All_mismatches":"22,19,18,16,15,12,10,9,8,7,5","Worst_Case_MismatchLocal":"2,2,2,2,2,2,2,2,2,2,2","Primer_Sequence":"ggcctctccacttaaacccagg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":3,"no_repeats_fw":2,"melting_temp":59.41,"Self_Dimer_DeltaG":-1.915,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":2,"Number_of_mismatches_hexamer":1,"_row":"290_openPrimeR2017"}
{"Primer":"Opt5_15","Template":"IGHV4-38-2","Group":"IGHV4-38-2","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-1.262,"Position_3terminus":1,"All_mismatches":"20,16,12,11,10,8,7,6,3,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"ggcctctccacttaaacccagg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":3,"no_repeats_fw":2,"melting_temp":59.41,"Self_Dimer_DeltaG":-1.915,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"291_openPrimeR2017"}
{"Primer":"Opt5_15","Template":"IGHV4-39","Group":"IGHV4-39","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-3.96,"Position_3terminus":7,"All_mismatches":"22,19,18,16,15,14,12,10,9,8,7","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0,0,0,0,0","Primer_Sequence":"ggcctctccacttaaacccagg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":3,"no_repeats_fw":2,"melting_temp":59.41,"Self_Dimer_DeltaG":-1.915,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"292_openPrimeR2017"}
{"Primer":"Opt5_15","Template":"IGHV4-4","Group":"IGHV4-4","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-1.548,"Position_3terminus":2,"All_mismatches":"20,19,17,16,15,14,13,10,8,5,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"ggcctctccacttaaacccagg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":3,"no_repeats_fw":2,"melting_temp":59.41,"Self_Dimer_DeltaG":-1.915,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":2,"_row":"293_openPrimeR2017"}
{"Primer":"Opt5_15","Template":"IGHV4-59","Group":"IGHV4-59","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-3.96,"Position_3terminus":7,"All_mismatches":"22,19,18,16,15,14,12,10,9,8,7","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0,0,0,0,0","Primer_Sequence":"ggcctctccacttaaacccagg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":3,"no_repeats_fw":2,"melting_temp":59.41,"Self_Dimer_DeltaG":-1.915,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"294_openPrimeR2017"}
{"Primer":"Opt5_15","Template":"IGHV4-61","Group":"IGHV4-61","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-3.96,"Position_3terminus":7,"All_mismatches":"22,19,18,16,15,14,12,10,9,8,7","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0,0,0,0,0","Primer_Sequence":"ggcctctccacttaaacccagg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":3,"no_repeats_fw":2,"melting_temp":59.41,"Self_Dimer_DeltaG":-1.915,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"295_openPrimeR2017"}
{"Primer":"Opt5_15","Template":"IGHV5-10-1","Group":"IGHV5-10-1","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-1.656,"Position_3terminus":1,"All_mismatches":"20,19,15,14,13,11,10,7,6,5,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"ggcctctccacttaaacccagg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":3,"no_repeats_fw":2,"melting_temp":59.41,"Self_Dimer_DeltaG":-1.915,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"296_openPrimeR2017"}
{"Primer":"Opt5_15","Template":"IGHV5-51","Group":"IGHV5-51","Number_of_mismatches":12,"primer_efficiency":0,"annealing_DeltaG":-0.194,"Position_3terminus":3,"All_mismatches":"20,19,16,15,13,11,10,8,7,6,4,3","Worst_Case_MismatchLocal":"4,4,4,4,4,4,4,4,4,4,4,4","Primer_Sequence":"ggcctctccacttaaacccagg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":3,"no_repeats_fw":2,"melting_temp":59.41,"Self_Dimer_DeltaG":-1.915,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":4,"Number_of_mismatches_hexamer":3,"_row":"297_openPrimeR2017"}
{"Primer":"Opt5_15","Template":"IGHV6-1","Group":"IGHV6-1","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-2.95,"Position_3terminus":6,"All_mismatches":"22,19,18,16,15,12,10,8,7,6","Worst_Case_MismatchLocal":"1,1,1,1,1,1,1,1,1,1","Primer_Sequence":"ggcctctccacttaaacccagg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":3,"no_repeats_fw":2,"melting_temp":59.41,"Self_Dimer_DeltaG":-1.915,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":1,"Number_of_mismatches_hexamer":1,"_row":"298_openPrimeR2017"}
{"Primer":"Opt5_15","Template":"IGHV7-4-1","Group":"IGHV7-4-1","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-3.076,"Position_3terminus":1,"All_mismatches":"20,19,16,13,12,11,10,7,5,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"ggcctctccacttaaacccagg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":3,"no_repeats_fw":2,"melting_temp":59.41,"Self_Dimer_DeltaG":-1.915,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"299_openPrimeR2017"}
{"Primer":"Opt5_16","Template":"IGHV1-18","Group":"IGHV1-18","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-3.082,"Position_3terminus":3,"All_mismatches":"21,20,17,15,14,13,12,11,10,9,3","Worst_Case_MismatchLocal":"4,4,4,4,4,4,4,4,4,4,4","Primer_Sequence":"tggacacactttgctacacactcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Unamplified","primer_length_fw":24,"gc_clamp_fw":2,"gc_ratio_fw":0.5,"no_runs_fw":3,"no_repeats_fw":3,"melting_temp":58.28,"Self_Dimer_DeltaG":-0.042,"Structure_deltaG_fw":0,"primer_coverage":45,"primer_specificity":0.55556,"Run":"openPrimeR2017","Position_3terminusLocal":4,"Number_of_mismatches_hexamer":1,"_row":"300_openPrimeR2017"}
{"Primer":"Opt5_16","Template":"IGHV1-2","Group":"IGHV1-2","Number_of_mismatches":12,"primer_efficiency":0,"annealing_DeltaG":-1.413,"Position_3terminus":1,"All_mismatches":"24,21,18,17,15,14,11,8,7,6,3,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"tggacacactttgctacacactcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":24,"gc_clamp_fw":2,"gc_ratio_fw":0.5,"no_runs_fw":3,"no_repeats_fw":3,"melting_temp":58.28,"Self_Dimer_DeltaG":-0.042,"Structure_deltaG_fw":0,"primer_coverage":45,"primer_specificity":0.55556,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"301_openPrimeR2017"}
{"Primer":"Opt5_16","Template":"IGHV1-24","Group":"IGHV1-24","Number_of_mismatches":12,"primer_efficiency":0,"annealing_DeltaG":-1.413,"Position_3terminus":1,"All_mismatches":"24,21,18,17,15,14,11,8,7,6,3,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"tggacacactttgctacacactcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":24,"gc_clamp_fw":2,"gc_ratio_fw":0.5,"no_runs_fw":3,"no_repeats_fw":3,"melting_temp":58.28,"Self_Dimer_DeltaG":-0.042,"Structure_deltaG_fw":0,"primer_coverage":45,"primer_specificity":0.55556,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"302_openPrimeR2017"}
{"Primer":"Opt5_16","Template":"IGHV1-3","Group":"IGHV1-3","Number_of_mismatches":12,"primer_efficiency":0,"annealing_DeltaG":-1.413,"Position_3terminus":1,"All_mismatches":"24,21,18,17,15,14,11,8,7,6,3,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"tggacacactttgctacacactcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":24,"gc_clamp_fw":2,"gc_ratio_fw":0.5,"no_runs_fw":3,"no_repeats_fw":3,"melting_temp":58.28,"Self_Dimer_DeltaG":-0.042,"Structure_deltaG_fw":0,"primer_coverage":45,"primer_specificity":0.55556,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"303_openPrimeR2017"}
{"Primer":"Opt5_16","Template":"IGHV1-45","Group":"IGHV1-45","Number_of_mismatches":12,"primer_efficiency":0,"annealing_DeltaG":-2.499,"Position_3terminus":1,"All_mismatches":"24,23,21,16,15,13,12,7,5,3,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"tggacacactttgctacacactcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":24,"gc_clamp_fw":2,"gc_ratio_fw":0.5,"no_runs_fw":3,"no_repeats_fw":3,"melting_temp":58.28,"Self_Dimer_DeltaG":-0.042,"Structure_deltaG_fw":0,"primer_coverage":45,"primer_specificity":0.55556,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":4,"_row":"304_openPrimeR2017"}
{"Primer":"Opt5_16","Template":"IGHV1-46","Group":"IGHV1-46","Number_of_mismatches":12,"primer_efficiency":0,"annealing_DeltaG":-1.413,"Position_3terminus":1,"All_mismatches":"24,21,18,17,15,14,11,8,7,6,3,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"tggacacactttgctacacactcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":24,"gc_clamp_fw":2,"gc_ratio_fw":0.5,"no_runs_fw":3,"no_repeats_fw":3,"melting_temp":58.28,"Self_Dimer_DeltaG":-0.042,"Structure_deltaG_fw":0,"primer_coverage":45,"primer_specificity":0.55556,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"305_openPrimeR2017"}
{"Primer":"Opt5_16","Template":"IGHV1-58","Group":"IGHV1-58","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-2.355,"Position_3terminus":1,"All_mismatches":"19,18,17,16,11,10,9,8,6,5,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"tggacacactttgctacacactcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":24,"gc_clamp_fw":2,"gc_ratio_fw":0.5,"no_runs_fw":3,"no_repeats_fw":3,"melting_temp":58.28,"Self_Dimer_DeltaG":-0.042,"Structure_deltaG_fw":0,"primer_coverage":45,"primer_specificity":0.55556,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"306_openPrimeR2017"}
{"Primer":"Opt5_16","Template":"IGHV1-69","Group":"IGHV1-69","Number_of_mismatches":12,"primer_efficiency":0,"annealing_DeltaG":-1.413,"Position_3terminus":1,"All_mismatches":"24,21,18,17,15,14,11,8,7,6,3,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"tggacacactttgctacacactcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":24,"gc_clamp_fw":2,"gc_ratio_fw":0.5,"no_runs_fw":3,"no_repeats_fw":3,"melting_temp":58.28,"Self_Dimer_DeltaG":-0.042,"Structure_deltaG_fw":0,"primer_coverage":45,"primer_specificity":0.55556,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"307_openPrimeR2017"}
{"Primer":"Opt5_16","Template":"IGHV1-69-2","Group":"IGHV1-69-2","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-1.787,"Position_3terminus":3,"All_mismatches":"23,22,21,17,16,15,14,10,6,5,3","Worst_Case_MismatchLocal":"4,4,4,4,4,4,4,4,4,4,4","Primer_Sequence":"tggacacactttgctacacactcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":24,"gc_clamp_fw":2,"gc_ratio_fw":0.5,"no_runs_fw":3,"no_repeats_fw":3,"melting_temp":58.28,"Self_Dimer_DeltaG":-0.042,"Structure_deltaG_fw":0,"primer_coverage":45,"primer_specificity":0.55556,"Run":"openPrimeR2017","Position_3terminusLocal":4,"Number_of_mismatches_hexamer":3,"_row":"308_openPrimeR2017"}
{"Primer":"Opt5_16","Template":"IGHV1-8","Group":"IGHV1-8","Number_of_mismatches":12,"primer_efficiency":0,"annealing_DeltaG":-1.413,"Position_3terminus":1,"All_mismatches":"24,21,18,17,15,14,11,8,7,6,3,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"tggacacactttgctacacactcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":24,"gc_clamp_fw":2,"gc_ratio_fw":0.5,"no_runs_fw":3,"no_repeats_fw":3,"melting_temp":58.28,"Self_Dimer_DeltaG":-0.042,"Structure_deltaG_fw":0,"primer_coverage":45,"primer_specificity":0.55556,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"309_openPrimeR2017"}
{"Primer":"Opt5_16","Template":"IGHV2-26","Group":"IGHV2-26","Number_of_mismatches":0,"primer_efficiency":0.99993,"annealing_DeltaG":-16.35,"All_mismatches":"NA","Worst_Case_MismatchLocal":"0","Primer_Sequence":"tggacacactttgctacacactcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":2,"gc_ratio_fw":0.5,"no_runs_fw":3,"no_repeats_fw":3,"melting_temp":58.28,"Self_Dimer_DeltaG":-0.042,"Structure_deltaG_fw":0,"primer_coverage":45,"primer_specificity":0.55556,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"310_openPrimeR2017"}
{"Primer":"Opt5_16","Template":"IGHV2-5","Group":"IGHV2-5","Number_of_mismatches":2,"primer_efficiency":0.92181,"annealing_DeltaG":-11.989,"Position_3terminus":5,"All_mismatches":"9,5","Worst_Case_MismatchLocal":"2,2","Primer_Sequence":"tggacacactttgctacacactcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":2,"gc_ratio_fw":0.5,"no_runs_fw":3,"no_repeats_fw":3,"melting_temp":58.28,"Self_Dimer_DeltaG":-0.042,"Structure_deltaG_fw":0,"primer_coverage":45,"primer_specificity":0.55556,"Run":"openPrimeR2017","Position_3terminusLocal":2,"Number_of_mismatches_hexamer":1,"_row":"311_openPrimeR2017"}
{"Primer":"Opt5_16","Template":"IGHV2-70","Group":"IGHV2-70","Number_of_mismatches":4,"primer_efficiency":0.00023,"annealing_DeltaG":-4.582,"Position_3terminus":5,"All_mismatches":"18,11,9,5","Worst_Case_MismatchLocal":"2,2,2,2","Primer_Sequence":"tggacacactttgctacacactcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Amplified","primer_length_fw":24,"gc_clamp_fw":2,"gc_ratio_fw":0.5,"no_runs_fw":3,"no_repeats_fw":3,"melting_temp":58.28,"Self_Dimer_DeltaG":-0.042,"Structure_deltaG_fw":0,"primer_coverage":45,"primer_specificity":0.55556,"Run":"openPrimeR2017","Position_3terminusLocal":2,"Number_of_mismatches_hexamer":1,"_row":"312_openPrimeR2017"}
{"Primer":"Opt5_16","Template":"IGHV3-11","Group":"IGHV3-11","Number_of_mismatches":12,"primer_efficiency":0,"annealing_DeltaG":-1.413,"Position_3terminus":1,"All_mismatches":"24,21,18,17,15,14,11,8,7,6,3,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"tggacacactttgctacacactcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":24,"gc_clamp_fw":2,"gc_ratio_fw":0.5,"no_runs_fw":3,"no_repeats_fw":3,"melting_temp":58.28,"Self_Dimer_DeltaG":-0.042,"Structure_deltaG_fw":0,"primer_coverage":45,"primer_specificity":0.55556,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"313_openPrimeR2017"}
{"Primer":"Opt5_16","Template":"IGHV3-13","Group":"IGHV3-13","Number_of_mismatches":12,"primer_efficiency":0,"annealing_DeltaG":-2.184,"Position_3terminus":1,"All_mismatches":"24,23,20,17,14,13,12,11,9,6,5,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"tggacacactttgctacacactcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":24,"gc_clamp_fw":2,"gc_ratio_fw":0.5,"no_runs_fw":3,"no_repeats_fw":3,"melting_temp":58.28,"Self_Dimer_DeltaG":-0.042,"Structure_deltaG_fw":0,"primer_coverage":45,"primer_specificity":0.55556,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"314_openPrimeR2017"}
{"Primer":"Opt5_16","Template":"IGHV3-15","Group":"IGHV3-15","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-3.262,"Position_3terminus":1,"All_mismatches":"20,19,12,11,10,9,8,5,3,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"tggacacactttgctacacactcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":24,"gc_clamp_fw":2,"gc_ratio_fw":0.5,"no_runs_fw":3,"no_repeats_fw":3,"melting_temp":58.28,"Self_Dimer_DeltaG":-0.042,"Structure_deltaG_fw":0,"primer_coverage":45,"primer_specificity":0.55556,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":4,"_row":"315_openPrimeR2017"}
{"Primer":"Opt5_16","Template":"IGHV3-20","Group":"IGHV3-20","Number_of_mismatches":12,"primer_efficiency":0,"annealing_DeltaG":-2.184,"Position_3terminus":1,"All_mismatches":"24,23,20,17,14,13,12,11,9,6,5,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"tggacacactttgctacacactcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":24,"gc_clamp_fw":2,"gc_ratio_fw":0.5,"no_runs_fw":3,"no_repeats_fw":3,"melting_temp":58.28,"Self_Dimer_DeltaG":-0.042,"Structure_deltaG_fw":0,"primer_coverage":45,"primer_specificity":0.55556,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"316_openPrimeR2017"}
{"Primer":"Opt5_16","Template":"IGHV3-21","Group":"IGHV3-21","Number_of_mismatches":12,"primer_efficiency":0,"annealing_DeltaG":-1.413,"Position_3terminus":1,"All_mismatches":"24,21,18,17,15,14,11,8,7,6,3,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"tggacacactttgctacacactcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":24,"gc_clamp_fw":2,"gc_ratio_fw":0.5,"no_runs_fw":3,"no_repeats_fw":3,"melting_temp":58.28,"Self_Dimer_DeltaG":-0.042,"Structure_deltaG_fw":0,"primer_coverage":45,"primer_specificity":0.55556,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"317_openPrimeR2017"}
{"Primer":"Opt5_16","Template":"IGHV3-23","Group":"IGHV3-23","Number_of_mismatches":12,"primer_efficiency":0,"annealing_DeltaG":-1.413,"Position_3terminus":1,"All_mismatches":"24,21,18,17,15,14,11,8,7,6,3,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"tggacacactttgctacacactcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":24,"gc_clamp_fw":2,"gc_ratio_fw":0.5,"no_runs_fw":3,"no_repeats_fw":3,"melting_temp":58.28,"Self_Dimer_DeltaG":-0.042,"Structure_deltaG_fw":0,"primer_coverage":45,"primer_specificity":0.55556,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"318_openPrimeR2017"}
{"Primer":"Opt5_16","Template":"IGHV3-30","Group":"IGHV3-30","Number_of_mismatches":12,"primer_efficiency":0,"annealing_DeltaG":-1.413,"Position_3terminus":1,"All_mismatches":"24,21,18,17,15,14,11,8,7,6,3,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"tggacacactttgctacacactcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":24,"gc_clamp_fw":2,"gc_ratio_fw":0.5,"no_runs_fw":3,"no_repeats_fw":3,"melting_temp":58.28,"Self_Dimer_DeltaG":-0.042,"Structure_deltaG_fw":0,"primer_coverage":45,"primer_specificity":0.55556,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"319_openPrimeR2017"}
{"Primer":"Opt5_16","Template":"IGHV3-30-3","Group":"IGHV3-30-3","Number_of_mismatches":12,"primer_efficiency":0,"annealing_DeltaG":-1.413,"Position_3terminus":1,"All_mismatches":"24,21,18,17,15,14,11,8,7,6,3,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"tggacacactttgctacacactcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":24,"gc_clamp_fw":2,"gc_ratio_fw":0.5,"no_runs_fw":3,"no_repeats_fw":3,"melting_temp":58.28,"Self_Dimer_DeltaG":-0.042,"Structure_deltaG_fw":0,"primer_coverage":45,"primer_specificity":0.55556,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"320_openPrimeR2017"}
{"Primer":"Opt5_16","Template":"IGHV3-33","Group":"IGHV3-33","Number_of_mismatches":12,"primer_efficiency":0,"annealing_DeltaG":-1.413,"Position_3terminus":1,"All_mismatches":"24,21,18,17,15,14,11,8,7,6,3,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"tggacacactttgctacacactcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":24,"gc_clamp_fw":2,"gc_ratio_fw":0.5,"no_runs_fw":3,"no_repeats_fw":3,"melting_temp":58.28,"Self_Dimer_DeltaG":-0.042,"Structure_deltaG_fw":0,"primer_coverage":45,"primer_specificity":0.55556,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"321_openPrimeR2017"}
{"Primer":"Opt5_16","Template":"IGHV3-43","Group":"IGHV3-43","Number_of_mismatches":12,"primer_efficiency":0,"annealing_DeltaG":-1.413,"Position_3terminus":1,"All_mismatches":"24,21,18,17,15,14,11,8,7,6,3,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"tggacacactttgctacacactcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":24,"gc_clamp_fw":2,"gc_ratio_fw":0.5,"no_runs_fw":3,"no_repeats_fw":3,"melting_temp":58.28,"Self_Dimer_DeltaG":-0.042,"Structure_deltaG_fw":0,"primer_coverage":45,"primer_specificity":0.55556,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"322_openPrimeR2017"}
{"Primer":"Opt5_16","Template":"IGHV3-48","Group":"IGHV3-48","Number_of_mismatches":12,"primer_efficiency":0,"annealing_DeltaG":-1.413,"Position_3terminus":1,"All_mismatches":"24,21,18,17,15,14,11,8,7,6,3,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"tggacacactttgctacacactcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":24,"gc_clamp_fw":2,"gc_ratio_fw":0.5,"no_runs_fw":3,"no_repeats_fw":3,"melting_temp":58.28,"Self_Dimer_DeltaG":-0.042,"Structure_deltaG_fw":0,"primer_coverage":45,"primer_specificity":0.55556,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"323_openPrimeR2017"}
{"Primer":"Opt5_16","Template":"IGHV3-49","Group":"IGHV3-49","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-1.413,"Position_3terminus":1,"All_mismatches":"24,21,18,17,15,11,8,7,6,3,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"tggacacactttgctacacactcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":24,"gc_clamp_fw":2,"gc_ratio_fw":0.5,"no_runs_fw":3,"no_repeats_fw":3,"melting_temp":58.28,"Self_Dimer_DeltaG":-0.042,"Structure_deltaG_fw":0,"primer_coverage":45,"primer_specificity":0.55556,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"324_openPrimeR2017"}
{"Primer":"Opt5_16","Template":"IGHV3-53","Group":"IGHV3-53","Number_of_mismatches":12,"primer_efficiency":0,"annealing_DeltaG":-1.413,"Position_3terminus":1,"All_mismatches":"24,21,18,17,15,14,11,8,7,6,3,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"tggacacactttgctacacactcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":24,"gc_clamp_fw":2,"gc_ratio_fw":0.5,"no_runs_fw":3,"no_repeats_fw":3,"melting_temp":58.28,"Self_Dimer_DeltaG":-0.042,"Structure_deltaG_fw":0,"primer_coverage":45,"primer_specificity":0.55556,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"325_openPrimeR2017"}
{"Primer":"Opt5_16","Template":"IGHV3-64","Group":"IGHV3-64","Number_of_mismatches":12,"primer_efficiency":0,"annealing_DeltaG":-1.413,"Position_3terminus":1,"All_mismatches":"24,21,18,17,15,14,11,8,7,6,3,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"tggacacactttgctacacactcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":24,"gc_clamp_fw":2,"gc_ratio_fw":0.5,"no_runs_fw":3,"no_repeats_fw":3,"melting_temp":58.28,"Self_Dimer_DeltaG":-0.042,"Structure_deltaG_fw":0,"primer_coverage":45,"primer_specificity":0.55556,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"326_openPrimeR2017"}
{"Primer":"Opt5_16","Template":"IGHV3-64D","Group":"IGHV3-64D","Number_of_mismatches":12,"primer_efficiency":0,"annealing_DeltaG":-1.413,"Position_3terminus":1,"All_mismatches":"24,21,18,17,15,14,11,8,7,6,3,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"tggacacactttgctacacactcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":24,"gc_clamp_fw":2,"gc_ratio_fw":0.5,"no_runs_fw":3,"no_repeats_fw":3,"melting_temp":58.28,"Self_Dimer_DeltaG":-0.042,"Structure_deltaG_fw":0,"primer_coverage":45,"primer_specificity":0.55556,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"327_openPrimeR2017"}
{"Primer":"Opt5_16","Template":"IGHV3-66","Group":"IGHV3-66","Number_of_mismatches":12,"primer_efficiency":0,"annealing_DeltaG":-1.413,"Position_3terminus":1,"All_mismatches":"24,21,18,17,15,14,11,8,7,6,3,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"tggacacactttgctacacactcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":24,"gc_clamp_fw":2,"gc_ratio_fw":0.5,"no_runs_fw":3,"no_repeats_fw":3,"melting_temp":58.28,"Self_Dimer_DeltaG":-0.042,"Structure_deltaG_fw":0,"primer_coverage":45,"primer_specificity":0.55556,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"328_openPrimeR2017"}
{"Primer":"Opt5_16","Template":"IGHV3-7","Group":"IGHV3-7","Number_of_mismatches":12,"primer_efficiency":0,"annealing_DeltaG":-1.413,"Position_3terminus":1,"All_mismatches":"24,21,18,17,15,14,11,8,7,6,3,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"tggacacactttgctacacactcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":24,"gc_clamp_fw":2,"gc_ratio_fw":0.5,"no_runs_fw":3,"no_repeats_fw":3,"melting_temp":58.28,"Self_Dimer_DeltaG":-0.042,"Structure_deltaG_fw":0,"primer_coverage":45,"primer_specificity":0.55556,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"329_openPrimeR2017"}
{"Primer":"Opt5_16","Template":"IGHV3-72","Group":"IGHV3-72","Number_of_mismatches":12,"primer_efficiency":0,"annealing_DeltaG":-2.184,"Position_3terminus":1,"All_mismatches":"24,23,20,17,14,13,12,11,9,6,5,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"tggacacactttgctacacactcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":24,"gc_clamp_fw":2,"gc_ratio_fw":0.5,"no_runs_fw":3,"no_repeats_fw":3,"melting_temp":58.28,"Self_Dimer_DeltaG":-0.042,"Structure_deltaG_fw":0,"primer_coverage":45,"primer_specificity":0.55556,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"330_openPrimeR2017"}
{"Primer":"Opt5_16","Template":"IGHV3-73","Group":"IGHV3-73","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-1.817,"Position_3terminus":1,"All_mismatches":"23,22,20,19,18,17,14,8,7,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"tggacacactttgctacacactcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":24,"gc_clamp_fw":2,"gc_ratio_fw":0.5,"no_runs_fw":3,"no_repeats_fw":3,"melting_temp":58.28,"Self_Dimer_DeltaG":-0.042,"Structure_deltaG_fw":0,"primer_coverage":45,"primer_specificity":0.55556,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":2,"_row":"331_openPrimeR2017"}
{"Primer":"Opt5_16","Template":"IGHV3-9","Group":"IGHV3-9","Number_of_mismatches":12,"primer_efficiency":0,"annealing_DeltaG":-1.413,"Position_3terminus":1,"All_mismatches":"24,21,18,17,15,14,11,8,7,6,3,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"tggacacactttgctacacactcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":24,"gc_clamp_fw":2,"gc_ratio_fw":0.5,"no_runs_fw":3,"no_repeats_fw":3,"melting_temp":58.28,"Self_Dimer_DeltaG":-0.042,"Structure_deltaG_fw":0,"primer_coverage":45,"primer_specificity":0.55556,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"332_openPrimeR2017"}
{"Primer":"Opt5_16","Template":"IGHV4-28","Group":"IGHV4-28","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-4.253,"Position_3terminus":1,"All_mismatches":"24,17,14,12,10,9,5,4,3,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"tggacacactttgctacacactcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":24,"gc_clamp_fw":2,"gc_ratio_fw":0.5,"no_runs_fw":3,"no_repeats_fw":3,"melting_temp":58.28,"Self_Dimer_DeltaG":-0.042,"Structure_deltaG_fw":0,"primer_coverage":45,"primer_specificity":0.55556,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":4,"_row":"333_openPrimeR2017"}
{"Primer":"Opt5_16","Template":"IGHV4-30-2","Group":"IGHV4-30-2","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-2.044,"Position_3terminus":5,"All_mismatches":"22,20,19,18,17,14,11,9,7,6,5","Worst_Case_MismatchLocal":"2,2,2,2,2,2,2,2,2,2,2","Primer_Sequence":"tggacacactttgctacacactcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":24,"gc_clamp_fw":2,"gc_ratio_fw":0.5,"no_runs_fw":3,"no_repeats_fw":3,"melting_temp":58.28,"Self_Dimer_DeltaG":-0.042,"Structure_deltaG_fw":0,"primer_coverage":45,"primer_specificity":0.55556,"Run":"openPrimeR2017","Position_3terminusLocal":2,"Number_of_mismatches_hexamer":2,"_row":"334_openPrimeR2017"}
{"Primer":"Opt5_16","Template":"IGHV4-31","Group":"IGHV4-31","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-2.322,"Position_3terminus":1,"All_mismatches":"19,18,17,14,10,8,6,5,4,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"tggacacactttgctacacactcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":5,"Experimental_Coverage":"Unamplified","primer_length_fw":24,"gc_clamp_fw":2,"gc_ratio_fw":0.5,"no_runs_fw":3,"no_repeats_fw":3,"melting_temp":58.28,"Self_Dimer_DeltaG":-0.042,"Structure_deltaG_fw":0,"primer_coverage":45,"primer_specificity":0.55556,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":5,"_row":"335_openPrimeR2017"}
{"Primer":"Opt5_16","Template":"IGHV4-34","Group":"IGHV4-34","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-2.044,"Position_3terminus":5,"All_mismatches":"22,20,19,18,17,14,11,9,7,6,5","Worst_Case_MismatchLocal":"2,2,2,2,2,2,2,2,2,2,2","Primer_Sequence":"tggacacactttgctacacactcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":24,"gc_clamp_fw":2,"gc_ratio_fw":0.5,"no_runs_fw":3,"no_repeats_fw":3,"melting_temp":58.28,"Self_Dimer_DeltaG":-0.042,"Structure_deltaG_fw":0,"primer_coverage":45,"primer_specificity":0.55556,"Run":"openPrimeR2017","Position_3terminusLocal":2,"Number_of_mismatches_hexamer":2,"_row":"336_openPrimeR2017"}
{"Primer":"Opt5_16","Template":"IGHV4-38-2","Group":"IGHV4-38-2","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-1.575,"Position_3terminus":1,"All_mismatches":"24,20,17,14,12,10,9,5,4,3,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"tggacacactttgctacacactcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":24,"gc_clamp_fw":2,"gc_ratio_fw":0.5,"no_runs_fw":3,"no_repeats_fw":3,"melting_temp":58.28,"Self_Dimer_DeltaG":-0.042,"Structure_deltaG_fw":0,"primer_coverage":45,"primer_specificity":0.55556,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":4,"_row":"337_openPrimeR2017"}
{"Primer":"Opt5_16","Template":"IGHV4-39","Group":"IGHV4-39","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-1.413,"Position_3terminus":5,"All_mismatches":"22,20,19,18,17,14,11,9,7,6,5","Worst_Case_MismatchLocal":"2,2,2,2,2,2,2,2,2,2,2","Primer_Sequence":"tggacacactttgctacacactcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":24,"gc_clamp_fw":2,"gc_ratio_fw":0.5,"no_runs_fw":3,"no_repeats_fw":3,"melting_temp":58.28,"Self_Dimer_DeltaG":-0.042,"Structure_deltaG_fw":0,"primer_coverage":45,"primer_specificity":0.55556,"Run":"openPrimeR2017","Position_3terminusLocal":2,"Number_of_mismatches_hexamer":2,"_row":"338_openPrimeR2017"}
{"Primer":"Opt5_16","Template":"IGHV4-4","Group":"IGHV4-4","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-1.949,"Position_3terminus":1,"All_mismatches":"21,20,18,12,11,10,8,5,3,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"tggacacactttgctacacactcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":24,"gc_clamp_fw":2,"gc_ratio_fw":0.5,"no_runs_fw":3,"no_repeats_fw":3,"melting_temp":58.28,"Self_Dimer_DeltaG":-0.042,"Structure_deltaG_fw":0,"primer_coverage":45,"primer_specificity":0.55556,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":4,"_row":"339_openPrimeR2017"}
{"Primer":"Opt5_16","Template":"IGHV4-59","Group":"IGHV4-59","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-2.322,"Position_3terminus":1,"All_mismatches":"19,18,17,14,10,8,6,5,4,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"tggacacactttgctacacactcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":5,"Experimental_Coverage":"Unamplified","primer_length_fw":24,"gc_clamp_fw":2,"gc_ratio_fw":0.5,"no_runs_fw":3,"no_repeats_fw":3,"melting_temp":58.28,"Self_Dimer_DeltaG":-0.042,"Structure_deltaG_fw":0,"primer_coverage":45,"primer_specificity":0.55556,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":5,"_row":"340_openPrimeR2017"}
{"Primer":"Opt5_16","Template":"IGHV4-61","Group":"IGHV4-61","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-2.322,"Position_3terminus":1,"All_mismatches":"19,18,17,14,10,8,6,5,4,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"tggacacactttgctacacactcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":5,"Experimental_Coverage":"Unamplified","primer_length_fw":24,"gc_clamp_fw":2,"gc_ratio_fw":0.5,"no_runs_fw":3,"no_repeats_fw":3,"melting_temp":58.28,"Self_Dimer_DeltaG":-0.042,"Structure_deltaG_fw":0,"primer_coverage":45,"primer_specificity":0.55556,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":5,"_row":"341_openPrimeR2017"}
{"Primer":"Opt5_16","Template":"IGHV5-51","Group":"IGHV5-51","Number_of_mismatches":12,"primer_efficiency":0,"annealing_DeltaG":-1.413,"Position_3terminus":1,"All_mismatches":"24,21,18,17,15,14,11,8,7,6,3,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"tggacacactttgctacacactcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":24,"gc_clamp_fw":2,"gc_ratio_fw":0.5,"no_runs_fw":3,"no_repeats_fw":3,"melting_temp":58.28,"Self_Dimer_DeltaG":-0.042,"Structure_deltaG_fw":0,"primer_coverage":45,"primer_specificity":0.55556,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"342_openPrimeR2017"}
{"Primer":"Opt5_16","Template":"IGHV6-1","Group":"IGHV6-1","Number_of_mismatches":12,"primer_efficiency":0,"annealing_DeltaG":-1.413,"Position_3terminus":1,"All_mismatches":"24,21,18,17,15,14,11,8,7,6,3,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"tggacacactttgctacacactcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":24,"gc_clamp_fw":2,"gc_ratio_fw":0.5,"no_runs_fw":3,"no_repeats_fw":3,"melting_temp":58.28,"Self_Dimer_DeltaG":-0.042,"Structure_deltaG_fw":0,"primer_coverage":45,"primer_specificity":0.55556,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"343_openPrimeR2017"}
{"Primer":"Opt5_16","Template":"IGHV7-4-1","Group":"IGHV7-4-1","Number_of_mismatches":12,"primer_efficiency":0,"annealing_DeltaG":-1.413,"Position_3terminus":1,"All_mismatches":"24,21,18,17,15,14,11,8,7,6,3,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"tggacacactttgctacacactcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":24,"gc_clamp_fw":2,"gc_ratio_fw":0.5,"no_runs_fw":3,"no_repeats_fw":3,"melting_temp":58.28,"Self_Dimer_DeltaG":-0.042,"Structure_deltaG_fw":0,"primer_coverage":45,"primer_specificity":0.55556,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"344_openPrimeR2017"}
{"Primer":"Opt5_2","Template":"IGHV1-18","Group":"IGHV1-18","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-2.334,"Position_3terminus":1,"All_mismatches":"21,17,16,13,12,11,10,6,4,3,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"cacctgtggttcttcctcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.73,"Self_Dimer_DeltaG":-0.81,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":4,"_row":"345_openPrimeR2017"}
{"Primer":"Opt5_2","Template":"IGHV1-2","Group":"IGHV1-2","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-2.026,"Position_3terminus":3,"All_mismatches":"22,19,16,13,12,10,7,6,3","Worst_Case_MismatchLocal":"4,4,4,4,4,4,4,4,4","Primer_Sequence":"cacctgtggttcttcctcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.73,"Self_Dimer_DeltaG":-0.81,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":4,"Number_of_mismatches_hexamer":2,"_row":"346_openPrimeR2017"}
{"Primer":"Opt5_2","Template":"IGHV1-24","Group":"IGHV1-24","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-2.217,"Position_3terminus":1,"All_mismatches":"22,21,19,18,16,13,10,7,4,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"cacctgtggttcttcctcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.73,"Self_Dimer_DeltaG":-0.81,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":2,"_row":"347_openPrimeR2017"}
{"Primer":"Opt5_2","Template":"IGHV1-3","Group":"IGHV1-3","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-1.481,"Position_3terminus":2,"All_mismatches":"22,19,16,13,12,10,9,7,3,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"cacctgtggttcttcctcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.73,"Self_Dimer_DeltaG":-0.81,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":2,"_row":"348_openPrimeR2017"}
{"Primer":"Opt5_2","Template":"IGHV1-45","Group":"IGHV1-45","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-2.217,"Position_3terminus":1,"All_mismatches":"22,21,19,18,16,14,13,10,7,4,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"cacctgtggttcttcctcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.73,"Self_Dimer_DeltaG":-0.81,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":2,"_row":"349_openPrimeR2017"}
{"Primer":"Opt5_2","Template":"IGHV1-46","Group":"IGHV1-46","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-2.29,"Position_3terminus":4,"All_mismatches":"22,21,19,18,16,13,7,6,4","Worst_Case_MismatchLocal":"3,3,3,3,3,3,3,3,3","Primer_Sequence":"cacctgtggttcttcctcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.73,"Self_Dimer_DeltaG":-0.81,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":3,"Number_of_mismatches_hexamer":2,"_row":"350_openPrimeR2017"}
{"Primer":"Opt5_2","Template":"IGHV1-58","Group":"IGHV1-58","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-4.653,"Position_3terminus":8,"All_mismatches":"22,17,16,14,13,12,11,10,9,8","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0,0,0,0","Primer_Sequence":"cacctgtggttcttcctcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.73,"Self_Dimer_DeltaG":-0.81,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"351_openPrimeR2017"}
{"Primer":"Opt5_2","Template":"IGHV1-69","Group":"IGHV1-69","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-3.034,"Position_3terminus":1,"All_mismatches":"22,21,19,18,16,10,7,5,4,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"cacctgtggttcttcctcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Amplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.73,"Self_Dimer_DeltaG":-0.81,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"352_openPrimeR2017"}
{"Primer":"Opt5_2","Template":"IGHV1-69-2","Group":"IGHV1-69-2","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-4.483,"Position_3terminus":1,"All_mismatches":"22,21,19,18,16,13,10,4,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6","Primer_Sequence":"cacctgtggttcttcctcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.73,"Self_Dimer_DeltaG":-0.81,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":2,"_row":"353_openPrimeR2017"}
{"Primer":"Opt5_2","Template":"IGHV1-8","Group":"IGHV1-8","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-2.217,"Position_3terminus":1,"All_mismatches":"22,21,19,18,16,13,10,7,4,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"cacctgtggttcttcctcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.73,"Self_Dimer_DeltaG":-0.81,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":2,"_row":"354_openPrimeR2017"}
{"Primer":"Opt5_2","Template":"IGHV2-26","Group":"IGHV2-26","Number_of_mismatches":9,"primer_efficiency":0.00008,"annealing_DeltaG":-3.916,"Position_3terminus":8,"All_mismatches":"22,21,20,17,14,12,10,9,8","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0,0,0","Primer_Sequence":"cacctgtggttcttcctcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.73,"Self_Dimer_DeltaG":-0.81,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"355_openPrimeR2017"}
{"Primer":"Opt5_2","Template":"IGHV2-5","Group":"IGHV2-5","Number_of_mismatches":9,"primer_efficiency":0.0001,"annealing_DeltaG":-4.033,"Position_3terminus":8,"All_mismatches":"22,21,20,17,14,12,10,9,8","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0,0,0","Primer_Sequence":"cacctgtggttcttcctcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.73,"Self_Dimer_DeltaG":-0.81,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"356_openPrimeR2017"}
{"Primer":"Opt5_2","Template":"IGHV2-70","Group":"IGHV2-70","Number_of_mismatches":9,"primer_efficiency":0.00003,"annealing_DeltaG":-3.356,"Position_3terminus":7,"All_mismatches":"22,19,16,13,12,11,9,8,7","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0,0,0","Primer_Sequence":"cacctgtggttcttcctcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.73,"Self_Dimer_DeltaG":-0.81,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"357_openPrimeR2017"}
{"Primer":"Opt5_2","Template":"IGHV3-11","Group":"IGHV3-11","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-2.052,"Position_3terminus":1,"All_mismatches":"21,20,19,18,17,13,11,5,4,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"cacctgtggttcttcctcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.73,"Self_Dimer_DeltaG":-0.81,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":4,"_row":"358_openPrimeR2017"}
{"Primer":"Opt5_2","Template":"IGHV3-13","Group":"IGHV3-13","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-4.189,"Position_3terminus":2,"All_mismatches":"21,14,13,12,10,7,6,5,4,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"cacctgtggttcttcctcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.73,"Self_Dimer_DeltaG":-0.81,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":4,"_row":"359_openPrimeR2017"}
{"Primer":"Opt5_2","Template":"IGHV3-15","Group":"IGHV3-15","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-4.189,"Position_3terminus":2,"All_mismatches":"21,14,13,12,10,7,6,5,4,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"cacctgtggttcttcctcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Amplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.73,"Self_Dimer_DeltaG":-0.81,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":4,"_row":"360_openPrimeR2017"}
{"Primer":"Opt5_2","Template":"IGHV3-20","Group":"IGHV3-20","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-3.302,"Position_3terminus":1,"All_mismatches":"22,20,19,12,10,9,5,4,3,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"cacctgtggttcttcctcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.73,"Self_Dimer_DeltaG":-0.81,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":4,"_row":"361_openPrimeR2017"}
{"Primer":"Opt5_2","Template":"IGHV3-21","Group":"IGHV3-21","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-1.62,"Position_3terminus":1,"All_mismatches":"22,19,16,13,12,8,7,6,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6","Primer_Sequence":"cacctgtggttcttcctcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.73,"Self_Dimer_DeltaG":-0.81,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":2,"_row":"362_openPrimeR2017"}
{"Primer":"Opt5_2","Template":"IGHV3-23","Group":"IGHV3-23","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-1.62,"Position_3terminus":1,"All_mismatches":"20,18,17,13,12,11,8,6,5,4,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"cacctgtggttcttcctcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.73,"Self_Dimer_DeltaG":-0.81,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":4,"_row":"363_openPrimeR2017"}
{"Primer":"Opt5_2","Template":"IGHV3-30","Group":"IGHV3-30","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-2.093,"Position_3terminus":1,"All_mismatches":"21,20,19,18,17,13,11,4,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"cacctgtggttcttcctcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.73,"Self_Dimer_DeltaG":-0.81,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"364_openPrimeR2017"}
{"Primer":"Opt5_2","Template":"IGHV3-30-3","Group":"IGHV3-30-3","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-2.093,"Position_3terminus":1,"All_mismatches":"21,20,19,18,17,13,11,4,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"cacctgtggttcttcctcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.73,"Self_Dimer_DeltaG":-0.81,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"365_openPrimeR2017"}
{"Primer":"Opt5_2","Template":"IGHV3-33","Group":"IGHV3-33","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-2.093,"Position_3terminus":1,"All_mismatches":"21,20,19,18,17,13,11,4,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"cacctgtggttcttcctcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.73,"Self_Dimer_DeltaG":-0.81,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"366_openPrimeR2017"}
{"Primer":"Opt5_2","Template":"IGHV3-43","Group":"IGHV3-43","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-2.052,"Position_3terminus":1,"All_mismatches":"21,20,19,18,17,13,11,5,4,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"cacctgtggttcttcctcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.73,"Self_Dimer_DeltaG":-0.81,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":4,"_row":"367_openPrimeR2017"}
{"Primer":"Opt5_2","Template":"IGHV3-48","Group":"IGHV3-48","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-2.052,"Position_3terminus":1,"All_mismatches":"22,21,20,14,12,11,10,8,7,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"cacctgtggttcttcctcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.73,"Self_Dimer_DeltaG":-0.81,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":2,"_row":"368_openPrimeR2017"}
{"Primer":"Opt5_2","Template":"IGHV3-49","Group":"IGHV3-49","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-1.769,"Position_3terminus":1,"All_mismatches":"21,19,18,16,14,10,9,7,5,4,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"cacctgtggttcttcctcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.73,"Self_Dimer_DeltaG":-0.81,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"369_openPrimeR2017"}
{"Primer":"Opt5_2","Template":"IGHV3-53","Group":"IGHV3-53","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-2.052,"Position_3terminus":1,"All_mismatches":"21,20,19,18,17,13,11,5,4,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"cacctgtggttcttcctcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.73,"Self_Dimer_DeltaG":-0.81,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":4,"_row":"370_openPrimeR2017"}
{"Primer":"Opt5_2","Template":"IGHV3-64","Group":"IGHV3-64","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-2.052,"Position_3terminus":1,"All_mismatches":"21,20,19,18,17,13,11,5,4,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"cacctgtggttcttcctcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.73,"Self_Dimer_DeltaG":-0.81,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":4,"_row":"371_openPrimeR2017"}
{"Primer":"Opt5_2","Template":"IGHV3-64D","Group":"IGHV3-64D","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-0.321,"Position_3terminus":1,"All_mismatches":"22,19,16,13,12,11,10,7,4,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"cacctgtggttcttcctcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.73,"Self_Dimer_DeltaG":-0.81,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":2,"_row":"372_openPrimeR2017"}
{"Primer":"Opt5_2","Template":"IGHV3-66","Group":"IGHV3-66","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-2.052,"Position_3terminus":1,"All_mismatches":"21,20,19,18,17,13,11,5,4,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"cacctgtggttcttcctcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.73,"Self_Dimer_DeltaG":-0.81,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":4,"_row":"373_openPrimeR2017"}
{"Primer":"Opt5_2","Template":"IGHV3-7","Group":"IGHV3-7","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-1.769,"Position_3terminus":3,"All_mismatches":"19,16,15,13,10,9,7,6,5,4,3","Worst_Case_MismatchLocal":"4,4,4,4,4,4,4,4,4,4,4","Primer_Sequence":"cacctgtggttcttcctcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.73,"Self_Dimer_DeltaG":-0.81,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":4,"Number_of_mismatches_hexamer":4,"_row":"374_openPrimeR2017"}
{"Primer":"Opt5_2","Template":"IGHV3-72","Group":"IGHV3-72","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-4.189,"Position_3terminus":2,"All_mismatches":"21,14,13,12,10,7,6,5,4,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"cacctgtggttcttcctcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.73,"Self_Dimer_DeltaG":-0.81,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":4,"_row":"375_openPrimeR2017"}
{"Primer":"Opt5_2","Template":"IGHV3-73","Group":"IGHV3-73","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-4.189,"Position_3terminus":2,"All_mismatches":"21,14,13,12,10,7,6,5,4,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"cacctgtggttcttcctcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.73,"Self_Dimer_DeltaG":-0.81,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":4,"_row":"376_openPrimeR2017"}
{"Primer":"Opt5_2","Template":"IGHV3-74","Group":"IGHV3-74","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-2.29,"Position_3terminus":1,"All_mismatches":"21,19,18,17,14,9,7,5,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6","Primer_Sequence":"cacctgtggttcttcctcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.73,"Self_Dimer_DeltaG":-0.81,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":2,"_row":"377_openPrimeR2017"}
{"Primer":"Opt5_2","Template":"IGHV3-9","Group":"IGHV3-9","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-2.052,"Position_3terminus":1,"All_mismatches":"21,20,19,18,17,13,11,5,4,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"cacctgtggttcttcctcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.73,"Self_Dimer_DeltaG":-0.81,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"378_openPrimeR2017"}
{"Primer":"Opt5_2","Template":"IGHV4-28","Group":"IGHV4-28","Number_of_mismatches":0,"primer_efficiency":0.99983,"annealing_DeltaG":-16.07,"All_mismatches":"NA","Worst_Case_MismatchLocal":"0","Primer_Sequence":"cacctgtggttcttcctcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.73,"Self_Dimer_DeltaG":-0.81,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"379_openPrimeR2017"}
{"Primer":"Opt5_2","Template":"IGHV4-30-2","Group":"IGHV4-30-2","Number_of_mismatches":0,"primer_efficiency":0.99983,"annealing_DeltaG":-16.07,"All_mismatches":"NA","Worst_Case_MismatchLocal":"0","Primer_Sequence":"cacctgtggttcttcctcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.73,"Self_Dimer_DeltaG":-0.81,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"380_openPrimeR2017"}
{"Primer":"Opt5_2","Template":"IGHV4-31","Group":"IGHV4-31","Number_of_mismatches":0,"primer_efficiency":0.99983,"annealing_DeltaG":-16.07,"All_mismatches":"NA","Worst_Case_MismatchLocal":"0","Primer_Sequence":"cacctgtggttcttcctcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.73,"Self_Dimer_DeltaG":-0.81,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"381_openPrimeR2017"}
{"Primer":"Opt5_2","Template":"IGHV4-34","Group":"IGHV4-34","Number_of_mismatches":1,"primer_efficiency":0.37134,"annealing_DeltaG":-14.539,"Position_3terminus":2,"All_mismatches":"2","Worst_Case_MismatchLocal":"5","Primer_Sequence":"cacctgtggttcttcctcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Amplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.73,"Self_Dimer_DeltaG":-0.81,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":1,"_row":"382_openPrimeR2017"}
{"Primer":"Opt5_2","Template":"IGHV4-38-2","Group":"IGHV4-38-2","Number_of_mismatches":8,"primer_efficiency":0.68995,"annealing_DeltaG":-10.932,"Position_3terminus":1,"All_mismatches":"11,9,7,6,4,3,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6","Primer_Sequence":"cacctgtggttcttcctcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":5,"Experimental_Coverage":"Amplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.73,"Self_Dimer_DeltaG":-0.81,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":5,"_row":"383_openPrimeR2017"}
{"Primer":"Opt5_2","Template":"IGHV4-39","Group":"IGHV4-39","Number_of_mismatches":0,"primer_efficiency":0.99983,"annealing_DeltaG":-16.07,"All_mismatches":"NA","Worst_Case_MismatchLocal":"0","Primer_Sequence":"cacctgtggttcttcctcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.73,"Self_Dimer_DeltaG":-0.81,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"384_openPrimeR2017"}
{"Primer":"Opt5_2","Template":"IGHV4-4","Group":"IGHV4-4","Number_of_mismatches":0,"primer_efficiency":0.99983,"annealing_DeltaG":-16.07,"All_mismatches":"NA","Worst_Case_MismatchLocal":"0","Primer_Sequence":"cacctgtggttcttcctcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.73,"Self_Dimer_DeltaG":-0.81,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"385_openPrimeR2017"}
{"Primer":"Opt5_2","Template":"IGHV4-59","Group":"IGHV4-59","Number_of_mismatches":3,"primer_efficiency":0,"annealing_DeltaG":-9.863,"Position_3terminus":2,"All_mismatches":"20,5,2","Worst_Case_MismatchLocal":"5,5,5","Primer_Sequence":"cacctgtggttcttcctcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Amplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.73,"Self_Dimer_DeltaG":-0.81,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":2,"_row":"386_openPrimeR2017"}
{"Primer":"Opt5_2","Template":"IGHV4-61","Group":"IGHV4-61","Number_of_mismatches":1,"primer_efficiency":0.37134,"annealing_DeltaG":-14.539,"Position_3terminus":2,"All_mismatches":"2","Worst_Case_MismatchLocal":"5","Primer_Sequence":"cacctgtggttcttcctcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Amplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.73,"Self_Dimer_DeltaG":-0.81,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":1,"_row":"387_openPrimeR2017"}
{"Primer":"Opt5_2","Template":"IGHV5-10-1","Group":"IGHV5-10-1","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-1.304,"Position_3terminus":2,"All_mismatches":"20,19,18,17,16,13,10,9,6,3,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"cacctgtggttcttcctcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.73,"Self_Dimer_DeltaG":-0.81,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":3,"_row":"388_openPrimeR2017"}
{"Primer":"Opt5_2","Template":"IGHV5-51","Group":"IGHV5-51","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-1.304,"Position_3terminus":2,"All_mismatches":"20,18,17,16,13,10,9,6,3,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"cacctgtggttcttcctcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.73,"Self_Dimer_DeltaG":-0.81,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":3,"_row":"389_openPrimeR2017"}
{"Primer":"Opt5_2","Template":"IGHV6-1","Group":"IGHV6-1","Number_of_mismatches":10,"primer_efficiency":0.00004,"annealing_DeltaG":-3.507,"Position_3terminus":7,"All_mismatches":"22,21,19,18,17,15,14,13,10,7","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0,0,0,0","Primer_Sequence":"cacctgtggttcttcctcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.73,"Self_Dimer_DeltaG":-0.81,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"390_openPrimeR2017"}
{"Primer":"Opt5_2","Template":"IGHV7-4-1","Group":"IGHV7-4-1","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-2.026,"Position_3terminus":2,"All_mismatches":"22,19,16,13,10,9,6,5,3,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"cacctgtggttcttcctcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":2,"melting_temp":59.73,"Self_Dimer_DeltaG":-0.81,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":4,"_row":"391_openPrimeR2017"}
{"Primer":"Opt5_3","Template":"IGHV1-18","Group":"IGHV1-18","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-3.183,"Position_3terminus":1,"All_mismatches":"21,15,13,12,9,8,7,4,3,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"atggagtttgggctgagctgg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":2,"gc_ratio_fw":0.57143,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":59.28,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"392_openPrimeR2017"}
{"Primer":"Opt5_3","Template":"IGHV1-2","Group":"IGHV1-2","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-4.465,"Position_3terminus":3,"All_mismatches":"21,18,15,13,11,8,6,5,4,3","Worst_Case_MismatchLocal":"4,4,4,4,4,4,4,4,4,4","Primer_Sequence":"atggagtttgggctgagctgg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":2,"gc_ratio_fw":0.57143,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":59.28,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":4,"Number_of_mismatches_hexamer":4,"_row":"393_openPrimeR2017"}
{"Primer":"Opt5_3","Template":"IGHV1-24","Group":"IGHV1-24","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-5.637,"Position_3terminus":1,"All_mismatches":"21,20,19,18,17,16,15,13,4,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"atggagtttgggctgagctgg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":2,"gc_ratio_fw":0.57143,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":59.28,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":2,"_row":"394_openPrimeR2017"}
{"Primer":"Opt5_3","Template":"IGHV1-3","Group":"IGHV1-3","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-2.666,"Position_3terminus":3,"All_mismatches":"21,19,18,15,11,8,6,5,4,3","Worst_Case_MismatchLocal":"4,4,4,4,4,4,4,4,4,4","Primer_Sequence":"atggagtttgggctgagctgg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":2,"gc_ratio_fw":0.57143,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":59.28,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":4,"Number_of_mismatches_hexamer":4,"_row":"395_openPrimeR2017"}
{"Primer":"Opt5_3","Template":"IGHV1-45","Group":"IGHV1-45","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-2.644,"Position_3terminus":5,"All_mismatches":"20,19,18,16,15,10,9,6,5","Worst_Case_MismatchLocal":"2,2,2,2,2,2,2,2,2","Primer_Sequence":"atggagtttgggctgagctgg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":2,"gc_ratio_fw":0.57143,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":59.28,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":2,"Number_of_mismatches_hexamer":2,"_row":"396_openPrimeR2017"}
{"Primer":"Opt5_3","Template":"IGHV1-46","Group":"IGHV1-46","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-4.465,"Position_3terminus":3,"All_mismatches":"21,18,15,13,11,8,6,5,4,3","Worst_Case_MismatchLocal":"4,4,4,4,4,4,4,4,4,4","Primer_Sequence":"atggagtttgggctgagctgg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":2,"gc_ratio_fw":0.57143,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":59.28,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":4,"Number_of_mismatches_hexamer":4,"_row":"397_openPrimeR2017"}
{"Primer":"Opt5_3","Template":"IGHV1-58","Group":"IGHV1-58","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-4.465,"Position_3terminus":3,"All_mismatches":"18,15,13,11,8,6,5,4,3","Worst_Case_MismatchLocal":"4,4,4,4,4,4,4,4,4","Primer_Sequence":"atggagtttgggctgagctgg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":2,"gc_ratio_fw":0.57143,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":59.28,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":4,"Number_of_mismatches_hexamer":4,"_row":"398_openPrimeR2017"}
{"Primer":"Opt5_3","Template":"IGHV1-69","Group":"IGHV1-69","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-1.962,"Position_3terminus":1,"All_mismatches":"21,15,12,11,10,7,6,4,3,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"atggagtttgggctgagctgg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":2,"gc_ratio_fw":0.57143,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":59.28,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":4,"_row":"399_openPrimeR2017"}
{"Primer":"Opt5_3","Template":"IGHV1-69-2","Group":"IGHV1-69-2","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-5.031,"Position_3terminus":2,"All_mismatches":"20,17,16,15,14,10,6,5,3,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"atggagtttgggctgagctgg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":2,"gc_ratio_fw":0.57143,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":59.28,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":4,"_row":"400_openPrimeR2017"}
{"Primer":"Opt5_3","Template":"IGHV1-8","Group":"IGHV1-8","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-4.465,"Position_3terminus":3,"All_mismatches":"21,18,15,13,11,8,6,5,4,3","Worst_Case_MismatchLocal":"4,4,4,4,4,4,4,4,4,4","Primer_Sequence":"atggagtttgggctgagctgg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":2,"gc_ratio_fw":0.57143,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":59.28,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":4,"Number_of_mismatches_hexamer":4,"_row":"401_openPrimeR2017"}
{"Primer":"Opt5_3","Template":"IGHV2-26","Group":"IGHV2-26","Number_of_mismatches":9,"primer_efficiency":0.00036,"annealing_DeltaG":-4.892,"Position_3terminus":6,"All_mismatches":"20,18,17,16,15,13,11,10,6","Worst_Case_MismatchLocal":"1,1,1,1,1,1,1,1,1","Primer_Sequence":"atggagtttgggctgagctgg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Amplified","primer_length_fw":21,"gc_clamp_fw":2,"gc_ratio_fw":0.57143,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":59.28,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":1,"Number_of_mismatches_hexamer":1,"_row":"402_openPrimeR2017"}
{"Primer":"Opt5_3","Template":"IGHV2-5","Group":"IGHV2-5","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-3.197,"Position_3terminus":2,"All_mismatches":"20,14,10,8,7,5,4,3,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5","Primer_Sequence":"atggagtttgggctgagctgg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Amplified","primer_length_fw":21,"gc_clamp_fw":2,"gc_ratio_fw":0.57143,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":59.28,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":4,"_row":"403_openPrimeR2017"}
{"Primer":"Opt5_3","Template":"IGHV2-70","Group":"IGHV2-70","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-4.413,"Position_3terminus":2,"All_mismatches":"20,14,10,8,7,6,5,4,3,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"atggagtttgggctgagctgg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":5,"Experimental_Coverage":"Amplified","primer_length_fw":21,"gc_clamp_fw":2,"gc_ratio_fw":0.57143,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":59.28,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":5,"_row":"404_openPrimeR2017"}
{"Primer":"Opt5_3","Template":"IGHV3-11","Group":"IGHV3-11","Number_of_mismatches":0,"primer_efficiency":0.99979,"annealing_DeltaG":-15.696,"All_mismatches":"NA","Worst_Case_MismatchLocal":"0","Primer_Sequence":"atggagtttgggctgagctgg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":21,"gc_clamp_fw":2,"gc_ratio_fw":0.57143,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":59.28,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"405_openPrimeR2017"}
{"Primer":"Opt5_3","Template":"IGHV3-13","Group":"IGHV3-13","Number_of_mismatches":1,"primer_efficiency":0.97741,"annealing_DeltaG":-12.617,"Position_3terminus":13,"All_mismatches":"13","Worst_Case_MismatchLocal":"0","Primer_Sequence":"atggagtttgggctgagctgg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":21,"gc_clamp_fw":2,"gc_ratio_fw":0.57143,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":59.28,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"406_openPrimeR2017"}
{"Primer":"Opt5_3","Template":"IGHV3-15","Group":"IGHV3-15","Number_of_mismatches":0,"primer_efficiency":0.99979,"annealing_DeltaG":-15.696,"All_mismatches":"NA","Worst_Case_MismatchLocal":"0","Primer_Sequence":"atggagtttgggctgagctgg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":21,"gc_clamp_fw":2,"gc_ratio_fw":0.57143,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":59.28,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"407_openPrimeR2017"}
{"Primer":"Opt5_3","Template":"IGHV3-20","Group":"IGHV3-20","Number_of_mismatches":0,"primer_efficiency":0.99979,"annealing_DeltaG":-15.696,"All_mismatches":"NA","Worst_Case_MismatchLocal":"0","Primer_Sequence":"atggagtttgggctgagctgg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":21,"gc_clamp_fw":2,"gc_ratio_fw":0.57143,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":59.28,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"408_openPrimeR2017"}
{"Primer":"Opt5_3","Template":"IGHV3-21","Group":"IGHV3-21","Number_of_mismatches":5,"primer_efficiency":0.00033,"annealing_DeltaG":-4.829,"Position_3terminus":6,"All_mismatches":"16,15,13,7,6","Worst_Case_MismatchLocal":"1,1,1,1,1","Primer_Sequence":"atggagtttgggctgagctgg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Amplified","primer_length_fw":21,"gc_clamp_fw":2,"gc_ratio_fw":0.57143,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":59.28,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":1,"Number_of_mismatches_hexamer":1,"_row":"409_openPrimeR2017"}
{"Primer":"Opt5_3","Template":"IGHV3-23","Group":"IGHV3-23","Number_of_mismatches":0,"primer_efficiency":0.99979,"annealing_DeltaG":-15.696,"All_mismatches":"NA","Worst_Case_MismatchLocal":"0","Primer_Sequence":"atggagtttgggctgagctgg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":21,"gc_clamp_fw":2,"gc_ratio_fw":0.57143,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":59.28,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"410_openPrimeR2017"}
{"Primer":"Opt5_3","Template":"IGHV3-30","Group":"IGHV3-30","Number_of_mismatches":0,"primer_efficiency":0.99979,"annealing_DeltaG":-15.696,"All_mismatches":"NA","Worst_Case_MismatchLocal":"0","Primer_Sequence":"atggagtttgggctgagctgg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":21,"gc_clamp_fw":2,"gc_ratio_fw":0.57143,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":59.28,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"411_openPrimeR2017"}
{"Primer":"Opt5_3","Template":"IGHV3-30-3","Group":"IGHV3-30-3","Number_of_mismatches":0,"primer_efficiency":0.99979,"annealing_DeltaG":-15.696,"All_mismatches":"NA","Worst_Case_MismatchLocal":"0","Primer_Sequence":"atggagtttgggctgagctgg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":21,"gc_clamp_fw":2,"gc_ratio_fw":0.57143,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":59.28,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"412_openPrimeR2017"}
{"Primer":"Opt5_3","Template":"IGHV3-33","Group":"IGHV3-33","Number_of_mismatches":0,"primer_efficiency":0.99979,"annealing_DeltaG":-15.696,"All_mismatches":"NA","Worst_Case_MismatchLocal":"0","Primer_Sequence":"atggagtttgggctgagctgg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":21,"gc_clamp_fw":2,"gc_ratio_fw":0.57143,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":59.28,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"413_openPrimeR2017"}
{"Primer":"Opt5_3","Template":"IGHV3-43","Group":"IGHV3-43","Number_of_mismatches":1,"primer_efficiency":0.98299,"annealing_DeltaG":-12.889,"Position_3terminus":10,"All_mismatches":"10","Worst_Case_MismatchLocal":"0","Primer_Sequence":"atggagtttgggctgagctgg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":21,"gc_clamp_fw":2,"gc_ratio_fw":0.57143,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":59.28,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"414_openPrimeR2017"}
{"Primer":"Opt5_3","Template":"IGHV3-48","Group":"IGHV3-48","Number_of_mismatches":2,"primer_efficiency":0.42641,"annealing_DeltaG":-9.967,"Position_3terminus":6,"All_mismatches":"13,6","Worst_Case_MismatchLocal":"1,1","Primer_Sequence":"atggagtttgggctgagctgg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Amplified","primer_length_fw":21,"gc_clamp_fw":2,"gc_ratio_fw":0.57143,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":59.28,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":1,"Number_of_mismatches_hexamer":1,"_row":"415_openPrimeR2017"}
{"Primer":"Opt5_3","Template":"IGHV3-49","Group":"IGHV3-49","Number_of_mismatches":1,"primer_efficiency":0.98131,"annealing_DeltaG":-12.743,"Position_3terminus":7,"All_mismatches":"7","Worst_Case_MismatchLocal":"0","Primer_Sequence":"atggagtttgggctgagctgg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":21,"gc_clamp_fw":2,"gc_ratio_fw":0.57143,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":59.28,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"416_openPrimeR2017"}
{"Primer":"Opt5_3","Template":"IGHV3-53","Group":"IGHV3-53","Number_of_mismatches":1,"primer_efficiency":0.97273,"annealing_DeltaG":-12.491,"Position_3terminus":12,"All_mismatches":"12","Worst_Case_MismatchLocal":"0","Primer_Sequence":"atggagtttgggctgagctgg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":21,"gc_clamp_fw":2,"gc_ratio_fw":0.57143,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":59.28,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"417_openPrimeR2017"}
{"Primer":"Opt5_3","Template":"IGHV3-64","Group":"IGHV3-64","Number_of_mismatches":1,"primer_efficiency":0.99911,"annealing_DeltaG":-14.74,"Position_3terminus":20,"All_mismatches":"20","Worst_Case_MismatchLocal":"0","Primer_Sequence":"atggagtttgggctgagctgg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":21,"gc_clamp_fw":2,"gc_ratio_fw":0.57143,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":59.28,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"418_openPrimeR2017"}
{"Primer":"Opt5_3","Template":"IGHV3-64D","Group":"IGHV3-64D","Number_of_mismatches":2,"primer_efficiency":0.76055,"annealing_DeltaG":-10.932,"Position_3terminus":12,"All_mismatches":"13,12","Worst_Case_MismatchLocal":"0,0","Primer_Sequence":"atggagtttgggctgagctgg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":21,"gc_clamp_fw":2,"gc_ratio_fw":0.57143,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":59.28,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"419_openPrimeR2017"}
{"Primer":"Opt5_3","Template":"IGHV3-66","Group":"IGHV3-66","Number_of_mismatches":0,"primer_efficiency":0.99979,"annealing_DeltaG":-15.696,"All_mismatches":"NA","Worst_Case_MismatchLocal":"0","Primer_Sequence":"atggagtttgggctgagctgg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":21,"gc_clamp_fw":2,"gc_ratio_fw":0.57143,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":59.28,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"420_openPrimeR2017"}
{"Primer":"Opt5_3","Template":"IGHV3-7","Group":"IGHV3-7","Number_of_mismatches":2,"primer_efficiency":0.35396,"annealing_DeltaG":-9.768,"Position_3terminus":13,"All_mismatches":"16,13","Worst_Case_MismatchLocal":"0,0","Primer_Sequence":"atggagtttgggctgagctgg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":21,"gc_clamp_fw":2,"gc_ratio_fw":0.57143,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":59.28,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"421_openPrimeR2017"}
{"Primer":"Opt5_3","Template":"IGHV3-72","Group":"IGHV3-72","Number_of_mismatches":0,"primer_efficiency":0.99979,"annealing_DeltaG":-15.696,"All_mismatches":"NA","Worst_Case_MismatchLocal":"0","Primer_Sequence":"atggagtttgggctgagctgg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":21,"gc_clamp_fw":2,"gc_ratio_fw":0.57143,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":59.28,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"422_openPrimeR2017"}
{"Primer":"Opt5_3","Template":"IGHV3-73","Group":"IGHV3-73","Number_of_mismatches":0,"primer_efficiency":0.99979,"annealing_DeltaG":-15.696,"All_mismatches":"NA","Worst_Case_MismatchLocal":"0","Primer_Sequence":"atggagtttgggctgagctgg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":21,"gc_clamp_fw":2,"gc_ratio_fw":0.57143,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":59.28,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"423_openPrimeR2017"}
{"Primer":"Opt5_3","Template":"IGHV3-74","Group":"IGHV3-74","Number_of_mismatches":0,"primer_efficiency":0.99979,"annealing_DeltaG":-15.696,"All_mismatches":"NA","Worst_Case_MismatchLocal":"0","Primer_Sequence":"atggagtttgggctgagctgg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":21,"gc_clamp_fw":2,"gc_ratio_fw":0.57143,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":59.28,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"424_openPrimeR2017"}
{"Primer":"Opt5_3","Template":"IGHV3-9","Group":"IGHV3-9","Number_of_mismatches":2,"primer_efficiency":0.33956,"annealing_DeltaG":-9.81,"Position_3terminus":10,"All_mismatches":"13,10","Worst_Case_MismatchLocal":"0,0","Primer_Sequence":"atggagtttgggctgagctgg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":21,"gc_clamp_fw":2,"gc_ratio_fw":0.57143,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":59.28,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"425_openPrimeR2017"}
{"Primer":"Opt5_3","Template":"IGHV4-28","Group":"IGHV4-28","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-2.097,"Position_3terminus":1,"All_mismatches":"15,13,12,10,9,8,7,5,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6","Primer_Sequence":"atggagtttgggctgagctgg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":2,"gc_ratio_fw":0.57143,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":59.28,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":2,"_row":"426_openPrimeR2017"}
{"Primer":"Opt5_3","Template":"IGHV4-30-2","Group":"IGHV4-30-2","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-2.707,"Position_3terminus":4,"All_mismatches":"21,17,15,14,13,11,10,8,7,4","Worst_Case_MismatchLocal":"3,3,3,3,3,3,3,3,3,3","Primer_Sequence":"atggagtttgggctgagctgg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Amplified","primer_length_fw":21,"gc_clamp_fw":2,"gc_ratio_fw":0.57143,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":59.28,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":3,"Number_of_mismatches_hexamer":1,"_row":"427_openPrimeR2017"}
{"Primer":"Opt5_3","Template":"IGHV4-31","Group":"IGHV4-31","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-2.707,"Position_3terminus":4,"All_mismatches":"21,20,17,15,13,12,11,7,5,4","Worst_Case_MismatchLocal":"3,3,3,3,3,3,3,3,3,3","Primer_Sequence":"atggagtttgggctgagctgg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Amplified","primer_length_fw":21,"gc_clamp_fw":2,"gc_ratio_fw":0.57143,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":59.28,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":3,"Number_of_mismatches_hexamer":2,"_row":"428_openPrimeR2017"}
{"Primer":"Opt5_3","Template":"IGHV4-34","Group":"IGHV4-34","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-2.707,"Position_3terminus":4,"All_mismatches":"21,17,15,14,13,11,10,8,7,4","Worst_Case_MismatchLocal":"3,3,3,3,3,3,3,3,3,3","Primer_Sequence":"atggagtttgggctgagctgg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":2,"gc_ratio_fw":0.57143,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":59.28,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":3,"Number_of_mismatches_hexamer":1,"_row":"429_openPrimeR2017"}
{"Primer":"Opt5_3","Template":"IGHV4-38-2","Group":"IGHV4-38-2","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-3.047,"Position_3terminus":6,"All_mismatches":"21,18,17,12,11,10,9,8,7,6","Worst_Case_MismatchLocal":"1,1,1,1,1,1,1,1,1,1","Primer_Sequence":"atggagtttgggctgagctgg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":2,"gc_ratio_fw":0.57143,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":59.28,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":1,"Number_of_mismatches_hexamer":1,"_row":"430_openPrimeR2017"}
{"Primer":"Opt5_3","Template":"IGHV4-39","Group":"IGHV4-39","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-2.655,"Position_3terminus":4,"All_mismatches":"21,17,15,14,13,11,10,8,7,4","Worst_Case_MismatchLocal":"3,3,3,3,3,3,3,3,3,3","Primer_Sequence":"atggagtttgggctgagctgg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":2,"gc_ratio_fw":0.57143,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":59.28,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":3,"Number_of_mismatches_hexamer":1,"_row":"431_openPrimeR2017"}
{"Primer":"Opt5_3","Template":"IGHV4-4","Group":"IGHV4-4","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-2.707,"Position_3terminus":4,"All_mismatches":"21,17,15,14,13,11,10,8,7,4","Worst_Case_MismatchLocal":"3,3,3,3,3,3,3,3,3,3","Primer_Sequence":"atggagtttgggctgagctgg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":2,"gc_ratio_fw":0.57143,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":59.28,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":3,"Number_of_mismatches_hexamer":1,"_row":"432_openPrimeR2017"}
{"Primer":"Opt5_3","Template":"IGHV4-59","Group":"IGHV4-59","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-2.707,"Position_3terminus":4,"All_mismatches":"21,17,15,14,13,11,10,8,7,4","Worst_Case_MismatchLocal":"3,3,3,3,3,3,3,3,3,3","Primer_Sequence":"atggagtttgggctgagctgg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":2,"gc_ratio_fw":0.57143,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":59.28,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":3,"Number_of_mismatches_hexamer":1,"_row":"433_openPrimeR2017"}
{"Primer":"Opt5_3","Template":"IGHV4-61","Group":"IGHV4-61","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-2.707,"Position_3terminus":4,"All_mismatches":"21,17,15,14,13,11,10,8,7,4","Worst_Case_MismatchLocal":"3,3,3,3,3,3,3,3,3,3","Primer_Sequence":"atggagtttgggctgagctgg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Amplified","primer_length_fw":21,"gc_clamp_fw":2,"gc_ratio_fw":0.57143,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":59.28,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":3,"Number_of_mismatches_hexamer":1,"_row":"434_openPrimeR2017"}
{"Primer":"Opt5_3","Template":"IGHV5-10-1","Group":"IGHV5-10-1","Number_of_mismatches":7,"primer_efficiency":0,"annealing_DeltaG":-3.841,"Position_3terminus":1,"All_mismatches":"20,14,11,8,5,4,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6","Primer_Sequence":"atggagtttgggctgagctgg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":2,"gc_ratio_fw":0.57143,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":59.28,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"435_openPrimeR2017"}
{"Primer":"Opt5_3","Template":"IGHV5-51","Group":"IGHV5-51","Number_of_mismatches":6,"primer_efficiency":0,"annealing_DeltaG":-4.518,"Position_3terminus":1,"All_mismatches":"20,14,11,8,4,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6","Primer_Sequence":"atggagtttgggctgagctgg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":2,"gc_ratio_fw":0.57143,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":59.28,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":2,"_row":"436_openPrimeR2017"}
{"Primer":"Opt5_3","Template":"IGHV6-1","Group":"IGHV6-1","Number_of_mismatches":8,"primer_efficiency":0,"annealing_DeltaG":-4.039,"Position_3terminus":3,"All_mismatches":"21,20,19,16,13,12,5,3","Worst_Case_MismatchLocal":"4,4,4,4,4,4,4,4","Primer_Sequence":"atggagtttgggctgagctgg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":2,"gc_ratio_fw":0.57143,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":59.28,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":4,"Number_of_mismatches_hexamer":2,"_row":"437_openPrimeR2017"}
{"Primer":"Opt5_3","Template":"IGHV7-4-1","Group":"IGHV7-4-1","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-2.881,"Position_3terminus":1,"All_mismatches":"20,19,18,16,14,12,9,8,7,3,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"atggagtttgggctgagctgg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":2,"gc_ratio_fw":0.57143,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":59.28,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":2,"_row":"438_openPrimeR2017"}
{"Primer":"Opt5_4","Template":"IGHV1-18","Group":"IGHV1-18","Number_of_mismatches":8,"primer_efficiency":0,"annealing_DeltaG":-4.808,"Position_3terminus":2,"All_mismatches":"20,14,11,8,7,6,3,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5","Primer_Sequence":"atggagttggggctgagctg","Terminal_Dinucleotide":"tg","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":20,"gc_clamp_fw":1,"gc_ratio_fw":0.6,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":59.3,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":3,"_row":"439_openPrimeR2017"}
{"Primer":"Opt5_4","Template":"IGHV1-2","Group":"IGHV1-2","Number_of_mismatches":8,"primer_efficiency":0.01026,"annealing_DeltaG":-7.489,"Position_3terminus":3,"All_mismatches":"20,19,18,17,16,15,14,3","Worst_Case_MismatchLocal":"4,4,4,4,4,4,4,4","Primer_Sequence":"atggagttggggctgagctg","Terminal_Dinucleotide":"tg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Amplified","primer_length_fw":20,"gc_clamp_fw":1,"gc_ratio_fw":0.6,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":59.3,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":4,"Number_of_mismatches_hexamer":1,"_row":"440_openPrimeR2017"}
{"Primer":"Opt5_4","Template":"IGHV1-24","Group":"IGHV1-24","Number_of_mismatches":8,"primer_efficiency":0.01046,"annealing_DeltaG":-7.489,"Position_3terminus":3,"All_mismatches":"20,19,18,17,16,15,14,3","Worst_Case_MismatchLocal":"4,4,4,4,4,4,4,4","Primer_Sequence":"atggagttggggctgagctg","Terminal_Dinucleotide":"tg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Amplified","primer_length_fw":20,"gc_clamp_fw":1,"gc_ratio_fw":0.6,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":59.3,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":4,"Number_of_mismatches_hexamer":1,"_row":"441_openPrimeR2017"}
{"Primer":"Opt5_4","Template":"IGHV1-3","Group":"IGHV1-3","Number_of_mismatches":8,"primer_efficiency":0.01026,"annealing_DeltaG":-7.489,"Position_3terminus":3,"All_mismatches":"20,19,18,17,16,15,14,3","Worst_Case_MismatchLocal":"4,4,4,4,4,4,4,4","Primer_Sequence":"atggagttggggctgagctg","Terminal_Dinucleotide":"tg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Amplified","primer_length_fw":20,"gc_clamp_fw":1,"gc_ratio_fw":0.6,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":59.3,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":4,"Number_of_mismatches_hexamer":1,"_row":"442_openPrimeR2017"}
{"Primer":"Opt5_4","Template":"IGHV1-45","Group":"IGHV1-45","Number_of_mismatches":8,"primer_efficiency":0,"annealing_DeltaG":-3.303,"Position_3terminus":2,"All_mismatches":"17,14,10,7,5,4,3,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5","Primer_Sequence":"atggagttggggctgagctg","Terminal_Dinucleotide":"tg","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Amplified","primer_length_fw":20,"gc_clamp_fw":1,"gc_ratio_fw":0.6,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":59.3,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":4,"_row":"443_openPrimeR2017"}
{"Primer":"Opt5_4","Template":"IGHV1-46","Group":"IGHV1-46","Number_of_mismatches":8,"primer_efficiency":0.01026,"annealing_DeltaG":-7.489,"Position_3terminus":3,"All_mismatches":"20,19,18,17,16,15,14,3","Worst_Case_MismatchLocal":"4,4,4,4,4,4,4,4","Primer_Sequence":"atggagttggggctgagctg","Terminal_Dinucleotide":"tg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Amplified","primer_length_fw":20,"gc_clamp_fw":1,"gc_ratio_fw":0.6,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":59.3,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":4,"Number_of_mismatches_hexamer":1,"_row":"444_openPrimeR2017"}
{"Primer":"Opt5_4","Template":"IGHV1-58","Group":"IGHV1-58","Number_of_mismatches":8,"primer_efficiency":0,"annealing_DeltaG":-3.303,"Position_3terminus":2,"All_mismatches":"17,14,10,7,5,4,3,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5","Primer_Sequence":"atggagttggggctgagctg","Terminal_Dinucleotide":"tg","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":20,"gc_clamp_fw":1,"gc_ratio_fw":0.6,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":59.3,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":4,"_row":"445_openPrimeR2017"}
{"Primer":"Opt5_4","Template":"IGHV1-69","Group":"IGHV1-69","Number_of_mismatches":8,"primer_efficiency":0.01026,"annealing_DeltaG":-7.489,"Position_3terminus":3,"All_mismatches":"20,19,18,17,16,15,14,3","Worst_Case_MismatchLocal":"4,4,4,4,4,4,4,4","Primer_Sequence":"atggagttggggctgagctg","Terminal_Dinucleotide":"tg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Amplified","primer_length_fw":20,"gc_clamp_fw":1,"gc_ratio_fw":0.6,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":59.3,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":4,"Number_of_mismatches_hexamer":1,"_row":"446_openPrimeR2017"}
{"Primer":"Opt5_4","Template":"IGHV1-69-2","Group":"IGHV1-69-2","Number_of_mismatches":8,"primer_efficiency":0.01046,"annealing_DeltaG":-7.489,"Position_3terminus":3,"All_mismatches":"20,19,18,17,16,15,14,3","Worst_Case_MismatchLocal":"4,4,4,4,4,4,4,4","Primer_Sequence":"atggagttggggctgagctg","Terminal_Dinucleotide":"tg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Amplified","primer_length_fw":20,"gc_clamp_fw":1,"gc_ratio_fw":0.6,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":59.3,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":4,"Number_of_mismatches_hexamer":1,"_row":"447_openPrimeR2017"}
{"Primer":"Opt5_4","Template":"IGHV1-8","Group":"IGHV1-8","Number_of_mismatches":8,"primer_efficiency":0.01026,"annealing_DeltaG":-7.489,"Position_3terminus":3,"All_mismatches":"20,19,18,17,16,15,14,3","Worst_Case_MismatchLocal":"4,4,4,4,4,4,4,4","Primer_Sequence":"atggagttggggctgagctg","Terminal_Dinucleotide":"tg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Amplified","primer_length_fw":20,"gc_clamp_fw":1,"gc_ratio_fw":0.6,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":59.3,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":4,"Number_of_mismatches_hexamer":1,"_row":"448_openPrimeR2017"}
{"Primer":"Opt5_4","Template":"IGHV2-26","Group":"IGHV2-26","Number_of_mismatches":8,"primer_efficiency":0.00001,"annealing_DeltaG":-2.759,"Position_3terminus":5,"All_mismatches":"19,17,16,15,14,10,9,5","Worst_Case_MismatchLocal":"2,2,2,2,2,2,2,2","Primer_Sequence":"atggagttggggctgagctg","Terminal_Dinucleotide":"tg","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Amplified","primer_length_fw":20,"gc_clamp_fw":1,"gc_ratio_fw":0.6,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":59.3,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":2,"Number_of_mismatches_hexamer":1,"_row":"449_openPrimeR2017"}
{"Primer":"Opt5_4","Template":"IGHV2-5","Group":"IGHV2-5","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-2.759,"Position_3terminus":5,"All_mismatches":"19,17,16,15,14,10,9,6,5","Worst_Case_MismatchLocal":"2,2,2,2,2,2,2,2,2","Primer_Sequence":"atggagttggggctgagctg","Terminal_Dinucleotide":"tg","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":20,"gc_clamp_fw":1,"gc_ratio_fw":0.6,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":59.3,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":2,"Number_of_mismatches_hexamer":2,"_row":"450_openPrimeR2017"}
{"Primer":"Opt5_4","Template":"IGHV2-70","Group":"IGHV2-70","Number_of_mismatches":9,"primer_efficiency":0.00001,"annealing_DeltaG":-2.723,"Position_3terminus":5,"All_mismatches":"20,19,17,16,15,14,10,9,5","Worst_Case_MismatchLocal":"2,2,2,2,2,2,2,2,2","Primer_Sequence":"atggagttggggctgagctg","Terminal_Dinucleotide":"tg","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Amplified","primer_length_fw":20,"gc_clamp_fw":1,"gc_ratio_fw":0.6,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":59.3,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":2,"Number_of_mismatches_hexamer":1,"_row":"451_openPrimeR2017"}
{"Primer":"Opt5_4","Template":"IGHV3-11","Group":"IGHV3-11","Number_of_mismatches":1,"primer_efficiency":0.95141,"annealing_DeltaG":-12.1,"Position_3terminus":12,"All_mismatches":"12","Worst_Case_MismatchLocal":"0","Primer_Sequence":"atggagttggggctgagctg","Terminal_Dinucleotide":"tg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":20,"gc_clamp_fw":1,"gc_ratio_fw":0.6,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":59.3,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"452_openPrimeR2017"}
{"Primer":"Opt5_4","Template":"IGHV3-13","Group":"IGHV3-13","Number_of_mismatches":0,"primer_efficiency":0.99963,"annealing_DeltaG":-15.305,"All_mismatches":"NA","Worst_Case_MismatchLocal":"0","Primer_Sequence":"atggagttggggctgagctg","Terminal_Dinucleotide":"tg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":20,"gc_clamp_fw":1,"gc_ratio_fw":0.6,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":59.3,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"453_openPrimeR2017"}
{"Primer":"Opt5_4","Template":"IGHV3-15","Group":"IGHV3-15","Number_of_mismatches":1,"primer_efficiency":0.95141,"annealing_DeltaG":-12.1,"Position_3terminus":12,"All_mismatches":"12","Worst_Case_MismatchLocal":"0","Primer_Sequence":"atggagttggggctgagctg","Terminal_Dinucleotide":"tg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":20,"gc_clamp_fw":1,"gc_ratio_fw":0.6,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":59.3,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"454_openPrimeR2017"}
{"Primer":"Opt5_4","Template":"IGHV3-20","Group":"IGHV3-20","Number_of_mismatches":1,"primer_efficiency":0.95141,"annealing_DeltaG":-12.1,"Position_3terminus":12,"All_mismatches":"12","Worst_Case_MismatchLocal":"0","Primer_Sequence":"atggagttggggctgagctg","Terminal_Dinucleotide":"tg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":20,"gc_clamp_fw":1,"gc_ratio_fw":0.6,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":59.3,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"455_openPrimeR2017"}
{"Primer":"Opt5_4","Template":"IGHV3-21","Group":"IGHV3-21","Number_of_mismatches":4,"primer_efficiency":0,"annealing_DeltaG":-5.963,"Position_3terminus":5,"All_mismatches":"15,14,6,5","Worst_Case_MismatchLocal":"2,2,2,2","Primer_Sequence":"atggagttggggctgagctg","Terminal_Dinucleotide":"tg","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Amplified","primer_length_fw":20,"gc_clamp_fw":1,"gc_ratio_fw":0.6,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":59.3,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":2,"Number_of_mismatches_hexamer":2,"_row":"456_openPrimeR2017"}
{"Primer":"Opt5_4","Template":"IGHV3-23","Group":"IGHV3-23","Number_of_mismatches":1,"primer_efficiency":0.95141,"annealing_DeltaG":-12.1,"Position_3terminus":12,"All_mismatches":"12","Worst_Case_MismatchLocal":"0","Primer_Sequence":"atggagttggggctgagctg","Terminal_Dinucleotide":"tg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":20,"gc_clamp_fw":1,"gc_ratio_fw":0.6,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":59.3,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"457_openPrimeR2017"}
{"Primer":"Opt5_4","Template":"IGHV3-30","Group":"IGHV3-30","Number_of_mismatches":1,"primer_efficiency":0.95141,"annealing_DeltaG":-12.1,"Position_3terminus":12,"All_mismatches":"12","Worst_Case_MismatchLocal":"0","Primer_Sequence":"atggagttggggctgagctg","Terminal_Dinucleotide":"tg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":20,"gc_clamp_fw":1,"gc_ratio_fw":0.6,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":59.3,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"458_openPrimeR2017"}
{"Primer":"Opt5_4","Template":"IGHV3-30-3","Group":"IGHV3-30-3","Number_of_mismatches":1,"primer_efficiency":0.95141,"annealing_DeltaG":-12.1,"Position_3terminus":12,"All_mismatches":"12","Worst_Case_MismatchLocal":"0","Primer_Sequence":"atggagttggggctgagctg","Terminal_Dinucleotide":"tg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":20,"gc_clamp_fw":1,"gc_ratio_fw":0.6,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":59.3,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"459_openPrimeR2017"}
{"Primer":"Opt5_4","Template":"IGHV3-33","Group":"IGHV3-33","Number_of_mismatches":1,"primer_efficiency":0.95141,"annealing_DeltaG":-12.1,"Position_3terminus":12,"All_mismatches":"12","Worst_Case_MismatchLocal":"0","Primer_Sequence":"atggagttggggctgagctg","Terminal_Dinucleotide":"tg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":20,"gc_clamp_fw":1,"gc_ratio_fw":0.6,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":59.3,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"460_openPrimeR2017"}
{"Primer":"Opt5_4","Template":"IGHV3-43","Group":"IGHV3-43","Number_of_mismatches":2,"primer_efficiency":0.18876,"annealing_DeltaG":-9.293,"Position_3terminus":9,"All_mismatches":"12,9","Worst_Case_MismatchLocal":"0,0","Primer_Sequence":"atggagttggggctgagctg","Terminal_Dinucleotide":"tg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":20,"gc_clamp_fw":1,"gc_ratio_fw":0.6,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":59.3,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"461_openPrimeR2017"}
{"Primer":"Opt5_4","Template":"IGHV3-48","Group":"IGHV3-48","Number_of_mismatches":1,"primer_efficiency":0.97866,"annealing_DeltaG":-12.655,"Position_3terminus":5,"All_mismatches":"5","Worst_Case_MismatchLocal":"2","Primer_Sequence":"atggagttggggctgagctg","Terminal_Dinucleotide":"tg","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Amplified","primer_length_fw":20,"gc_clamp_fw":1,"gc_ratio_fw":0.6,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":59.3,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":2,"Number_of_mismatches_hexamer":1,"_row":"462_openPrimeR2017"}
{"Primer":"Opt5_4","Template":"IGHV3-49","Group":"IGHV3-49","Number_of_mismatches":2,"primer_efficiency":0.1745,"annealing_DeltaG":-9.147,"Position_3terminus":6,"All_mismatches":"12,6","Worst_Case_MismatchLocal":"1,1","Primer_Sequence":"atggagttggggctgagctg","Terminal_Dinucleotide":"tg","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Amplified","primer_length_fw":20,"gc_clamp_fw":1,"gc_ratio_fw":0.6,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":59.3,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":1,"Number_of_mismatches_hexamer":1,"_row":"463_openPrimeR2017"}
{"Primer":"Opt5_4","Template":"IGHV3-53","Group":"IGHV3-53","Number_of_mismatches":2,"primer_efficiency":0.2004,"annealing_DeltaG":-9.258,"Position_3terminus":11,"All_mismatches":"12,11","Worst_Case_MismatchLocal":"0,0","Primer_Sequence":"atggagttggggctgagctg","Terminal_Dinucleotide":"tg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":20,"gc_clamp_fw":1,"gc_ratio_fw":0.6,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":59.3,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"464_openPrimeR2017"}
{"Primer":"Opt5_4","Template":"IGHV3-64","Group":"IGHV3-64","Number_of_mismatches":2,"primer_efficiency":0.81884,"annealing_DeltaG":-11.144,"Position_3terminus":12,"All_mismatches":"19,12","Worst_Case_MismatchLocal":"0,0","Primer_Sequence":"atggagttggggctgagctg","Terminal_Dinucleotide":"tg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":20,"gc_clamp_fw":1,"gc_ratio_fw":0.6,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":59.3,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"465_openPrimeR2017"}
{"Primer":"Opt5_4","Template":"IGHV3-64D","Group":"IGHV3-64D","Number_of_mismatches":2,"primer_efficiency":0.196,"annealing_DeltaG":-9.258,"Position_3terminus":11,"All_mismatches":"12,11","Worst_Case_MismatchLocal":"0,0","Primer_Sequence":"atggagttggggctgagctg","Terminal_Dinucleotide":"tg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":20,"gc_clamp_fw":1,"gc_ratio_fw":0.6,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":59.3,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"466_openPrimeR2017"}
{"Primer":"Opt5_4","Template":"IGHV3-66","Group":"IGHV3-66","Number_of_mismatches":1,"primer_efficiency":0.95141,"annealing_DeltaG":-12.1,"Position_3terminus":12,"All_mismatches":"12","Worst_Case_MismatchLocal":"0","Primer_Sequence":"atggagttggggctgagctg","Terminal_Dinucleotide":"tg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":20,"gc_clamp_fw":1,"gc_ratio_fw":0.6,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":59.3,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"467_openPrimeR2017"}
{"Primer":"Opt5_4","Template":"IGHV3-7","Group":"IGHV3-7","Number_of_mismatches":1,"primer_efficiency":0.97127,"annealing_DeltaG":-12.456,"Position_3terminus":15,"All_mismatches":"15","Worst_Case_MismatchLocal":"0","Primer_Sequence":"atggagttggggctgagctg","Terminal_Dinucleotide":"tg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":20,"gc_clamp_fw":1,"gc_ratio_fw":0.6,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":59.3,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"468_openPrimeR2017"}
{"Primer":"Opt5_4","Template":"IGHV3-72","Group":"IGHV3-72","Number_of_mismatches":1,"primer_efficiency":0.95141,"annealing_DeltaG":-12.1,"Position_3terminus":12,"All_mismatches":"12","Worst_Case_MismatchLocal":"0","Primer_Sequence":"atggagttggggctgagctg","Terminal_Dinucleotide":"tg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":20,"gc_clamp_fw":1,"gc_ratio_fw":0.6,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":59.3,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"469_openPrimeR2017"}
{"Primer":"Opt5_4","Template":"IGHV3-73","Group":"IGHV3-73","Number_of_mismatches":1,"primer_efficiency":0.95141,"annealing_DeltaG":-12.1,"Position_3terminus":12,"All_mismatches":"12","Worst_Case_MismatchLocal":"0","Primer_Sequence":"atggagttggggctgagctg","Terminal_Dinucleotide":"tg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":20,"gc_clamp_fw":1,"gc_ratio_fw":0.6,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":59.3,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"470_openPrimeR2017"}
{"Primer":"Opt5_4","Template":"IGHV3-74","Group":"IGHV3-74","Number_of_mismatches":1,"primer_efficiency":0.95141,"annealing_DeltaG":-12.1,"Position_3terminus":12,"All_mismatches":"12","Worst_Case_MismatchLocal":"0","Primer_Sequence":"atggagttggggctgagctg","Terminal_Dinucleotide":"tg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":20,"gc_clamp_fw":1,"gc_ratio_fw":0.6,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":59.3,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"471_openPrimeR2017"}
{"Primer":"Opt5_4","Template":"IGHV3-9","Group":"IGHV3-9","Number_of_mismatches":1,"primer_efficiency":0.96944,"annealing_DeltaG":-12.498,"Position_3terminus":9,"All_mismatches":"9","Worst_Case_MismatchLocal":"0","Primer_Sequence":"atggagttggggctgagctg","Terminal_Dinucleotide":"tg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":20,"gc_clamp_fw":1,"gc_ratio_fw":0.6,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":59.3,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"472_openPrimeR2017"}
{"Primer":"Opt5_4","Template":"IGHV4-28","Group":"IGHV4-28","Number_of_mismatches":8,"primer_efficiency":0,"annealing_DeltaG":-2.097,"Position_3terminus":4,"All_mismatches":"14,12,11,9,8,7,6,4","Worst_Case_MismatchLocal":"3,3,3,3,3,3,3,3","Primer_Sequence":"atggagttggggctgagctg","Terminal_Dinucleotide":"tg","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":20,"gc_clamp_fw":1,"gc_ratio_fw":0.6,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":59.3,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":3,"Number_of_mismatches_hexamer":2,"_row":"473_openPrimeR2017"}
{"Primer":"Opt5_4","Template":"IGHV4-30-2","Group":"IGHV4-30-2","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-1.67,"Position_3terminus":2,"All_mismatches":"19,17,13,11,10,9,7,6,5,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"atggagttggggctgagctg","Terminal_Dinucleotide":"tg","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Amplified","primer_length_fw":20,"gc_clamp_fw":1,"gc_ratio_fw":0.6,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":59.3,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":3,"_row":"474_openPrimeR2017"}
{"Primer":"Opt5_4","Template":"IGHV4-31","Group":"IGHV4-31","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-1.67,"Position_3terminus":2,"All_mismatches":"19,17,13,11,10,9,7,6,5,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"atggagttggggctgagctg","Terminal_Dinucleotide":"tg","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":20,"gc_clamp_fw":1,"gc_ratio_fw":0.6,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":59.3,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":3,"_row":"475_openPrimeR2017"}
{"Primer":"Opt5_4","Template":"IGHV4-34","Group":"IGHV4-34","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-1.67,"Position_3terminus":2,"All_mismatches":"19,17,13,11,10,9,7,6,5,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"atggagttggggctgagctg","Terminal_Dinucleotide":"tg","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":20,"gc_clamp_fw":1,"gc_ratio_fw":0.6,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":59.3,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":3,"_row":"476_openPrimeR2017"}
{"Primer":"Opt5_4","Template":"IGHV4-38-2","Group":"IGHV4-38-2","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-1.67,"Position_3terminus":1,"All_mismatches":"19,17,15,14,11,8,6,5,4,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"atggagttggggctgagctg","Terminal_Dinucleotide":"tg","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":20,"gc_clamp_fw":1,"gc_ratio_fw":0.6,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":59.3,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":4,"_row":"477_openPrimeR2017"}
{"Primer":"Opt5_4","Template":"IGHV4-39","Group":"IGHV4-39","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-2.655,"Position_3terminus":3,"All_mismatches":"20,16,14,13,10,9,7,6,3","Worst_Case_MismatchLocal":"4,4,4,4,4,4,4,4,4","Primer_Sequence":"atggagttggggctgagctg","Terminal_Dinucleotide":"tg","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":20,"gc_clamp_fw":1,"gc_ratio_fw":0.6,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":59.3,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":4,"Number_of_mismatches_hexamer":2,"_row":"478_openPrimeR2017"}
{"Primer":"Opt5_4","Template":"IGHV4-4","Group":"IGHV4-4","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-1.67,"Position_3terminus":2,"All_mismatches":"19,17,13,11,10,9,7,6,5,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"atggagttggggctgagctg","Terminal_Dinucleotide":"tg","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":20,"gc_clamp_fw":1,"gc_ratio_fw":0.6,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":59.3,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":3,"_row":"479_openPrimeR2017"}
{"Primer":"Opt5_4","Template":"IGHV4-59","Group":"IGHV4-59","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-1.67,"Position_3terminus":2,"All_mismatches":"19,17,13,11,10,9,7,6,5,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"atggagttggggctgagctg","Terminal_Dinucleotide":"tg","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":20,"gc_clamp_fw":1,"gc_ratio_fw":0.6,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":59.3,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":3,"_row":"480_openPrimeR2017"}
{"Primer":"Opt5_4","Template":"IGHV4-61","Group":"IGHV4-61","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-1.67,"Position_3terminus":2,"All_mismatches":"19,17,13,11,10,9,7,6,5,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"atggagttggggctgagctg","Terminal_Dinucleotide":"tg","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Amplified","primer_length_fw":20,"gc_clamp_fw":1,"gc_ratio_fw":0.6,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":59.3,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":3,"_row":"481_openPrimeR2017"}
{"Primer":"Opt5_4","Template":"IGHV5-10-1","Group":"IGHV5-10-1","Number_of_mismatches":7,"primer_efficiency":0,"annealing_DeltaG":-2.759,"Position_3terminus":3,"All_mismatches":"19,13,12,10,7,4,3","Worst_Case_MismatchLocal":"4,4,4,4,4,4,4","Primer_Sequence":"atggagttggggctgagctg","Terminal_Dinucleotide":"tg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":20,"gc_clamp_fw":1,"gc_ratio_fw":0.6,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":59.3,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":4,"Number_of_mismatches_hexamer":2,"_row":"482_openPrimeR2017"}
{"Primer":"Opt5_4","Template":"IGHV5-51","Group":"IGHV5-51","Number_of_mismatches":6,"primer_efficiency":8.72335e-06,"annealing_DeltaG":-2.759,"Position_3terminus":3,"All_mismatches":"19,13,12,10,7,3","Worst_Case_MismatchLocal":"4,4,4,4,4,4","Primer_Sequence":"atggagttggggctgagctg","Terminal_Dinucleotide":"tg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Unamplified","primer_length_fw":20,"gc_clamp_fw":1,"gc_ratio_fw":0.6,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":59.3,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":4,"Number_of_mismatches_hexamer":1,"_row":"483_openPrimeR2017"}
{"Primer":"Opt5_4","Template":"IGHV6-1","Group":"IGHV6-1","Number_of_mismatches":8,"primer_efficiency":0,"annealing_DeltaG":-4.039,"Position_3terminus":2,"All_mismatches":"20,19,18,15,12,11,4,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5","Primer_Sequence":"atggagttggggctgagctg","Terminal_Dinucleotide":"tg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":20,"gc_clamp_fw":1,"gc_ratio_fw":0.6,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":59.3,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":2,"_row":"484_openPrimeR2017"}
{"Primer":"Opt5_4","Template":"IGHV7-4-1","Group":"IGHV7-4-1","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-1.67,"Position_3terminus":2,"All_mismatches":"19,17,13,11,10,9,7,6,5,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"atggagttggggctgagctg","Terminal_Dinucleotide":"tg","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":20,"gc_clamp_fw":1,"gc_ratio_fw":0.6,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":59.3,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":3,"_row":"485_openPrimeR2017"}
{"Primer":"Opt5_5","Template":"IGHV1-18","Group":"IGHV1-18","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-3.183,"Position_3terminus":1,"All_mismatches":"16,14,13,10,9,8,5,4,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"tggagttttggctgagctggg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":3,"gc_ratio_fw":0.57143,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":59.43,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":-0.12,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":4,"_row":"486_openPrimeR2017"}
{"Primer":"Opt5_5","Template":"IGHV1-2","Group":"IGHV1-2","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-4.6,"Position_3terminus":4,"All_mismatches":"19,16,14,13,12,9,7,6,5,4","Worst_Case_MismatchLocal":"3,3,3,3,3,3,3,3,3,3","Primer_Sequence":"tggagttttggctgagctggg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":3,"gc_ratio_fw":0.57143,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":59.43,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":-0.12,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":3,"Number_of_mismatches_hexamer":3,"_row":"487_openPrimeR2017"}
{"Primer":"Opt5_5","Template":"IGHV1-24","Group":"IGHV1-24","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-1.559,"Position_3terminus":2,"All_mismatches":"21,20,19,17,15,13,10,9,8,4,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"tggagttttggctgagctggg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":3,"gc_ratio_fw":0.57143,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":59.43,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":-0.12,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":2,"_row":"488_openPrimeR2017"}
{"Primer":"Opt5_5","Template":"IGHV1-3","Group":"IGHV1-3","Number_of_mismatches":10,"primer_efficiency":0.00003,"annealing_DeltaG":-3.312,"Position_3terminus":4,"All_mismatches":"20,19,16,13,12,9,7,6,5,4","Worst_Case_MismatchLocal":"3,3,3,3,3,3,3,3,3,3","Primer_Sequence":"tggagttttggctgagctggg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":3,"gc_ratio_fw":0.57143,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":59.43,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":-0.12,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":3,"Number_of_mismatches_hexamer":3,"_row":"489_openPrimeR2017"}
{"Primer":"Opt5_5","Template":"IGHV1-45","Group":"IGHV1-45","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-4.6,"Position_3terminus":4,"All_mismatches":"19,16,14,13,12,9,7,6,5,4","Worst_Case_MismatchLocal":"3,3,3,3,3,3,3,3,3,3","Primer_Sequence":"tggagttttggctgagctggg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":3,"gc_ratio_fw":0.57143,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":59.43,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":-0.12,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":3,"Number_of_mismatches_hexamer":3,"_row":"490_openPrimeR2017"}
{"Primer":"Opt5_5","Template":"IGHV1-46","Group":"IGHV1-46","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-4.6,"Position_3terminus":4,"All_mismatches":"19,16,14,13,12,9,7,6,5,4","Worst_Case_MismatchLocal":"3,3,3,3,3,3,3,3,3,3","Primer_Sequence":"tggagttttggctgagctggg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":3,"gc_ratio_fw":0.57143,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":59.43,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":-0.12,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":3,"Number_of_mismatches_hexamer":3,"_row":"491_openPrimeR2017"}
{"Primer":"Opt5_5","Template":"IGHV1-58","Group":"IGHV1-58","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-1.522,"Position_3terminus":1,"All_mismatches":"21,20,19,18,16,15,14,10,7,6,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"tggagttttggctgagctggg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":3,"gc_ratio_fw":0.57143,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":59.43,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":-0.12,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":2,"_row":"492_openPrimeR2017"}
{"Primer":"Opt5_5","Template":"IGHV1-69","Group":"IGHV1-69","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-1.705,"Position_3terminus":2,"All_mismatches":"16,13,12,11,8,7,5,4,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5","Primer_Sequence":"tggagttttggctgagctggg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":3,"gc_ratio_fw":0.57143,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":59.43,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":-0.12,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":3,"_row":"493_openPrimeR2017"}
{"Primer":"Opt5_5","Template":"IGHV1-69-2","Group":"IGHV1-69-2","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-2.384,"Position_3terminus":6,"All_mismatches":"21,20,17,15,14,12,11,10,7,6","Worst_Case_MismatchLocal":"1,1,1,1,1,1,1,1,1,1","Primer_Sequence":"tggagttttggctgagctggg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":3,"gc_ratio_fw":0.57143,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":59.43,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":-0.12,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":1,"Number_of_mismatches_hexamer":1,"_row":"494_openPrimeR2017"}
{"Primer":"Opt5_5","Template":"IGHV1-8","Group":"IGHV1-8","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-4.6,"Position_3terminus":4,"All_mismatches":"19,16,14,13,12,9,7,6,5,4","Worst_Case_MismatchLocal":"3,3,3,3,3,3,3,3,3,3","Primer_Sequence":"tggagttttggctgagctggg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":3,"gc_ratio_fw":0.57143,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":59.43,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":-0.12,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":3,"Number_of_mismatches_hexamer":3,"_row":"495_openPrimeR2017"}
{"Primer":"Opt5_5","Template":"IGHV2-26","Group":"IGHV2-26","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-1.559,"Position_3terminus":2,"All_mismatches":"21,20,19,17,15,13,10,9,8,4,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"tggagttttggctgagctggg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":3,"gc_ratio_fw":0.57143,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":59.43,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":-0.12,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":2,"_row":"496_openPrimeR2017"}
{"Primer":"Opt5_5","Template":"IGHV2-5","Group":"IGHV2-5","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-2.761,"Position_3terminus":3,"All_mismatches":"21,15,13,11,9,8,6,5,4,3","Worst_Case_MismatchLocal":"4,4,4,4,4,4,4,4,4,4","Primer_Sequence":"tggagttttggctgagctggg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":3,"gc_ratio_fw":0.57143,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":59.43,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":-0.12,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":4,"Number_of_mismatches_hexamer":4,"_row":"497_openPrimeR2017"}
{"Primer":"Opt5_5","Template":"IGHV2-70","Group":"IGHV2-70","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-1.559,"Position_3terminus":2,"All_mismatches":"21,20,19,17,15,13,10,9,8,4,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"tggagttttggctgagctggg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":3,"gc_ratio_fw":0.57143,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":59.43,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":-0.12,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":2,"_row":"498_openPrimeR2017"}
{"Primer":"Opt5_5","Template":"IGHV3-11","Group":"IGHV3-11","Number_of_mismatches":1,"primer_efficiency":0.97873,"annealing_DeltaG":-12.703,"Position_3terminus":13,"All_mismatches":"13","Worst_Case_MismatchLocal":"0","Primer_Sequence":"tggagttttggctgagctggg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":21,"gc_clamp_fw":3,"gc_ratio_fw":0.57143,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":59.43,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":-0.12,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"499_openPrimeR2017"}
{"Primer":"Opt5_5","Template":"IGHV3-13","Group":"IGHV3-13","Number_of_mismatches":2,"primer_efficiency":0.792,"annealing_DeltaG":-11.078,"Position_3terminus":13,"All_mismatches":"14,13","Worst_Case_MismatchLocal":"0,0","Primer_Sequence":"tggagttttggctgagctggg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":21,"gc_clamp_fw":3,"gc_ratio_fw":0.57143,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":59.43,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":-0.12,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"500_openPrimeR2017"}
{"Primer":"Opt5_5","Template":"IGHV3-15","Group":"IGHV3-15","Number_of_mismatches":2,"primer_efficiency":0.02034,"annealing_DeltaG":-11.841,"Position_3terminus":1,"All_mismatches":"13,1","Worst_Case_MismatchLocal":"6,6","Primer_Sequence":"tggagttttggctgagctggg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Amplified","primer_length_fw":21,"gc_clamp_fw":3,"gc_ratio_fw":0.57143,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":59.43,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":-0.12,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":1,"_row":"501_openPrimeR2017"}
{"Primer":"Opt5_5","Template":"IGHV3-20","Group":"IGHV3-20","Number_of_mismatches":1,"primer_efficiency":0.97873,"annealing_DeltaG":-12.703,"Position_3terminus":13,"All_mismatches":"13","Worst_Case_MismatchLocal":"0","Primer_Sequence":"tggagttttggctgagctggg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":21,"gc_clamp_fw":3,"gc_ratio_fw":0.57143,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":59.43,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":-0.12,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"502_openPrimeR2017"}
{"Primer":"Opt5_5","Template":"IGHV3-21","Group":"IGHV3-21","Number_of_mismatches":6,"primer_efficiency":0.00033,"annealing_DeltaG":-4.829,"Position_3terminus":7,"All_mismatches":"17,16,14,13,8,7","Worst_Case_MismatchLocal":"0,0,0,0,0,0","Primer_Sequence":"tggagttttggctgagctggg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":21,"gc_clamp_fw":3,"gc_ratio_fw":0.57143,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":59.43,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":-0.12,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"503_openPrimeR2017"}
{"Primer":"Opt5_5","Template":"IGHV3-23","Group":"IGHV3-23","Number_of_mismatches":2,"primer_efficiency":0.02146,"annealing_DeltaG":-11.926,"Position_3terminus":1,"All_mismatches":"13,1","Worst_Case_MismatchLocal":"6,6","Primer_Sequence":"tggagttttggctgagctggg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Amplified","primer_length_fw":21,"gc_clamp_fw":3,"gc_ratio_fw":0.57143,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":59.43,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":-0.12,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":1,"_row":"504_openPrimeR2017"}
{"Primer":"Opt5_5","Template":"IGHV3-30","Group":"IGHV3-30","Number_of_mismatches":1,"primer_efficiency":0.97873,"annealing_DeltaG":-12.703,"Position_3terminus":13,"All_mismatches":"13","Worst_Case_MismatchLocal":"0","Primer_Sequence":"tggagttttggctgagctggg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":21,"gc_clamp_fw":3,"gc_ratio_fw":0.57143,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":59.43,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":-0.12,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"505_openPrimeR2017"}
{"Primer":"Opt5_5","Template":"IGHV3-30-3","Group":"IGHV3-30-3","Number_of_mismatches":1,"primer_efficiency":0.97873,"annealing_DeltaG":-12.703,"Position_3terminus":13,"All_mismatches":"13","Worst_Case_MismatchLocal":"0","Primer_Sequence":"tggagttttggctgagctggg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":21,"gc_clamp_fw":3,"gc_ratio_fw":0.57143,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":59.43,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":-0.12,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"506_openPrimeR2017"}
{"Primer":"Opt5_5","Template":"IGHV3-33","Group":"IGHV3-33","Number_of_mismatches":1,"primer_efficiency":0.97873,"annealing_DeltaG":-12.703,"Position_3terminus":13,"All_mismatches":"13","Worst_Case_MismatchLocal":"0","Primer_Sequence":"tggagttttggctgagctggg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":21,"gc_clamp_fw":3,"gc_ratio_fw":0.57143,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":59.43,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":-0.12,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"507_openPrimeR2017"}
{"Primer":"Opt5_5","Template":"IGHV3-43","Group":"IGHV3-43","Number_of_mismatches":2,"primer_efficiency":0.3535,"annealing_DeltaG":-9.896,"Position_3terminus":11,"All_mismatches":"13,11","Worst_Case_MismatchLocal":"0,0","Primer_Sequence":"tggagttttggctgagctggg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":21,"gc_clamp_fw":3,"gc_ratio_fw":0.57143,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":59.43,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":-0.12,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"508_openPrimeR2017"}
{"Primer":"Opt5_5","Template":"IGHV3-48","Group":"IGHV3-48","Number_of_mismatches":3,"primer_efficiency":0.0614,"annealing_DeltaG":-8.428,"Position_3terminus":7,"All_mismatches":"14,13,7","Worst_Case_MismatchLocal":"0,0,0","Primer_Sequence":"tggagttttggctgagctggg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":21,"gc_clamp_fw":3,"gc_ratio_fw":0.57143,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":59.43,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":-0.12,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"509_openPrimeR2017"}
{"Primer":"Opt5_5","Template":"IGHV3-49","Group":"IGHV3-49","Number_of_mismatches":2,"primer_efficiency":0.3319,"annealing_DeltaG":-9.75,"Position_3terminus":8,"All_mismatches":"13,8","Worst_Case_MismatchLocal":"0,0","Primer_Sequence":"tggagttttggctgagctggg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":21,"gc_clamp_fw":3,"gc_ratio_fw":0.57143,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":59.43,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":-0.12,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"510_openPrimeR2017"}
{"Primer":"Opt5_5","Template":"IGHV3-53","Group":"IGHV3-53","Number_of_mismatches":0,"primer_efficiency":0.99981,"annealing_DeltaG":-15.782,"All_mismatches":"NA","Worst_Case_MismatchLocal":"0","Primer_Sequence":"tggagttttggctgagctggg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":21,"gc_clamp_fw":3,"gc_ratio_fw":0.57143,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":59.43,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":-0.12,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"511_openPrimeR2017"}
{"Primer":"Opt5_5","Template":"IGHV3-64","Group":"IGHV3-64","Number_of_mismatches":2,"primer_efficiency":0.94431,"annealing_DeltaG":-12.052,"Position_3terminus":13,"All_mismatches":"21,13","Worst_Case_MismatchLocal":"0,0","Primer_Sequence":"tggagttttggctgagctggg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":21,"gc_clamp_fw":3,"gc_ratio_fw":0.57143,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":59.43,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":-0.12,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"512_openPrimeR2017"}
{"Primer":"Opt5_5","Template":"IGHV3-64D","Group":"IGHV3-64D","Number_of_mismatches":1,"primer_efficiency":0.99301,"annealing_DeltaG":-13.456,"Position_3terminus":14,"All_mismatches":"14","Worst_Case_MismatchLocal":"0","Primer_Sequence":"tggagttttggctgagctggg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":21,"gc_clamp_fw":3,"gc_ratio_fw":0.57143,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":59.43,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":-0.12,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"513_openPrimeR2017"}
{"Primer":"Opt5_5","Template":"IGHV3-66","Group":"IGHV3-66","Number_of_mismatches":1,"primer_efficiency":0.97873,"annealing_DeltaG":-12.703,"Position_3terminus":13,"All_mismatches":"13","Worst_Case_MismatchLocal":"0","Primer_Sequence":"tggagttttggctgagctggg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":21,"gc_clamp_fw":3,"gc_ratio_fw":0.57143,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":59.43,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":-0.12,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"514_openPrimeR2017"}
{"Primer":"Opt5_5","Template":"IGHV3-7","Group":"IGHV3-7","Number_of_mismatches":3,"primer_efficiency":0.31911,"annealing_DeltaG":-9.712,"Position_3terminus":13,"All_mismatches":"17,14,13","Worst_Case_MismatchLocal":"0,0,0","Primer_Sequence":"tggagttttggctgagctggg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":21,"gc_clamp_fw":3,"gc_ratio_fw":0.57143,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":59.43,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":-0.12,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"515_openPrimeR2017"}
{"Primer":"Opt5_5","Template":"IGHV3-72","Group":"IGHV3-72","Number_of_mismatches":1,"primer_efficiency":0.97873,"annealing_DeltaG":-12.703,"Position_3terminus":13,"All_mismatches":"13","Worst_Case_MismatchLocal":"0","Primer_Sequence":"tggagttttggctgagctggg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":21,"gc_clamp_fw":3,"gc_ratio_fw":0.57143,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":59.43,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":-0.12,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"516_openPrimeR2017"}
{"Primer":"Opt5_5","Template":"IGHV3-73","Group":"IGHV3-73","Number_of_mismatches":1,"primer_efficiency":0.97873,"annealing_DeltaG":-12.703,"Position_3terminus":13,"All_mismatches":"13","Worst_Case_MismatchLocal":"0","Primer_Sequence":"tggagttttggctgagctggg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":21,"gc_clamp_fw":3,"gc_ratio_fw":0.57143,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":59.43,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":-0.12,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"517_openPrimeR2017"}
{"Primer":"Opt5_5","Template":"IGHV3-74","Group":"IGHV3-74","Number_of_mismatches":1,"primer_efficiency":0.97873,"annealing_DeltaG":-12.703,"Position_3terminus":13,"All_mismatches":"13","Worst_Case_MismatchLocal":"0","Primer_Sequence":"tggagttttggctgagctggg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":21,"gc_clamp_fw":3,"gc_ratio_fw":0.57143,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":59.43,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":-0.12,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"518_openPrimeR2017"}
{"Primer":"Opt5_5","Template":"IGHV3-9","Group":"IGHV3-9","Number_of_mismatches":4,"primer_efficiency":0.00037,"annealing_DeltaG":-7.409,"Position_3terminus":1,"All_mismatches":"14,13,11,1","Worst_Case_MismatchLocal":"6,6,6,6","Primer_Sequence":"tggagttttggctgagctggg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Amplified","primer_length_fw":21,"gc_clamp_fw":3,"gc_ratio_fw":0.57143,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":59.43,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":-0.12,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":1,"_row":"519_openPrimeR2017"}
{"Primer":"Opt5_5","Template":"IGHV4-28","Group":"IGHV4-28","Number_of_mismatches":8,"primer_efficiency":0,"annealing_DeltaG":-1.802,"Position_3terminus":2,"All_mismatches":"16,14,11,10,9,8,6,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5","Primer_Sequence":"tggagttttggctgagctggg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":3,"gc_ratio_fw":0.57143,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":59.43,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":-0.12,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":2,"_row":"520_openPrimeR2017"}
{"Primer":"Opt5_5","Template":"IGHV4-30-2","Group":"IGHV4-30-2","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-2.707,"Position_3terminus":5,"All_mismatches":"18,16,15,14,13,12,11,9,8,5","Worst_Case_MismatchLocal":"2,2,2,2,2,2,2,2,2,2","Primer_Sequence":"tggagttttggctgagctggg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":3,"gc_ratio_fw":0.57143,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":59.43,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":-0.12,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":2,"Number_of_mismatches_hexamer":1,"_row":"521_openPrimeR2017"}
{"Primer":"Opt5_5","Template":"IGHV4-31","Group":"IGHV4-31","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-2.707,"Position_3terminus":5,"All_mismatches":"21,18,16,14,13,12,8,6,5","Worst_Case_MismatchLocal":"2,2,2,2,2,2,2,2,2","Primer_Sequence":"tggagttttggctgagctggg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Amplified","primer_length_fw":21,"gc_clamp_fw":3,"gc_ratio_fw":0.57143,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":59.43,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":-0.12,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":2,"Number_of_mismatches_hexamer":2,"_row":"522_openPrimeR2017"}
{"Primer":"Opt5_5","Template":"IGHV4-34","Group":"IGHV4-34","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-2.575,"Position_3terminus":1,"All_mismatches":"21,15,14,12,11,5,4,3,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"tggagttttggctgagctggg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":5,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":3,"gc_ratio_fw":0.57143,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":59.43,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":-0.12,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":5,"_row":"523_openPrimeR2017"}
{"Primer":"Opt5_5","Template":"IGHV4-38-2","Group":"IGHV4-38-2","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-3.728,"Position_3terminus":1,"All_mismatches":"19,18,12,11,10,9,8,7,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6","Primer_Sequence":"tggagttttggctgagctggg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":3,"gc_ratio_fw":0.57143,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":59.43,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":-0.12,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":1,"_row":"524_openPrimeR2017"}
{"Primer":"Opt5_5","Template":"IGHV4-39","Group":"IGHV4-39","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-1.49,"Position_3terminus":2,"All_mismatches":"21,20,15,14,10,8,7,6,5,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"tggagttttggctgagctggg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":3,"gc_ratio_fw":0.57143,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":59.43,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":-0.12,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":3,"_row":"525_openPrimeR2017"}
{"Primer":"Opt5_5","Template":"IGHV4-4","Group":"IGHV4-4","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-2.707,"Position_3terminus":5,"All_mismatches":"18,16,15,14,13,12,11,9,8,5","Worst_Case_MismatchLocal":"2,2,2,2,2,2,2,2,2,2","Primer_Sequence":"tggagttttggctgagctggg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":3,"gc_ratio_fw":0.57143,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":59.43,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":-0.12,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":2,"Number_of_mismatches_hexamer":1,"_row":"526_openPrimeR2017"}
{"Primer":"Opt5_5","Template":"IGHV4-59","Group":"IGHV4-59","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-1.647,"Position_3terminus":2,"All_mismatches":"21,20,15,14,10,9,8,7,6,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"tggagttttggctgagctggg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":3,"gc_ratio_fw":0.57143,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":59.43,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":-0.12,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":2,"_row":"527_openPrimeR2017"}
{"Primer":"Opt5_5","Template":"IGHV4-61","Group":"IGHV4-61","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-1.647,"Position_3terminus":2,"All_mismatches":"21,20,15,14,10,9,8,7,6,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"tggagttttggctgagctggg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Amplified","primer_length_fw":21,"gc_clamp_fw":3,"gc_ratio_fw":0.57143,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":59.43,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":-0.12,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":2,"_row":"528_openPrimeR2017"}
{"Primer":"Opt5_5","Template":"IGHV5-10-1","Group":"IGHV5-10-1","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-2.759,"Position_3terminus":1,"All_mismatches":"21,15,13,12,9,6,5,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6","Primer_Sequence":"tggagttttggctgagctggg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":3,"gc_ratio_fw":0.57143,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":59.43,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":-0.12,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":4,"_row":"529_openPrimeR2017"}
{"Primer":"Opt5_5","Template":"IGHV5-51","Group":"IGHV5-51","Number_of_mismatches":8,"primer_efficiency":0,"annealing_DeltaG":-2.759,"Position_3terminus":1,"All_mismatches":"21,15,13,12,9,5,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6","Primer_Sequence":"tggagttttggctgagctggg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":3,"gc_ratio_fw":0.57143,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":59.43,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":-0.12,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"530_openPrimeR2017"}
{"Primer":"Opt5_5","Template":"IGHV6-1","Group":"IGHV6-1","Number_of_mismatches":6,"primer_efficiency":0,"annealing_DeltaG":-4.264,"Position_3terminus":4,"All_mismatches":"21,20,17,14,6,4","Worst_Case_MismatchLocal":"3,3,3,3,3,3","Primer_Sequence":"tggagttttggctgagctggg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Amplified","primer_length_fw":21,"gc_clamp_fw":3,"gc_ratio_fw":0.57143,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":59.43,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":-0.12,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":3,"Number_of_mismatches_hexamer":2,"_row":"531_openPrimeR2017"}
{"Primer":"Opt5_5","Template":"IGHV7-4-1","Group":"IGHV7-4-1","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-1.559,"Position_3terminus":2,"All_mismatches":"21,20,19,17,15,13,10,9,8,4,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"tggagttttggctgagctggg","Terminal_Dinucleotide":"gg","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":3,"gc_ratio_fw":0.57143,"no_runs_fw":4,"no_repeats_fw":2,"melting_temp":59.43,"Self_Dimer_DeltaG":-2.255,"Structure_deltaG_fw":-0.12,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":2,"_row":"532_openPrimeR2017"}
{"Primer":"Opt5_6","Template":"IGHV1-18","Group":"IGHV1-18","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-3.945,"Position_3terminus":1,"All_mismatches":"20,18,16,14,11,10,9,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6","Primer_Sequence":"actttgctccacgctcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":20,"gc_clamp_fw":2,"gc_ratio_fw":0.6,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":60.07,"Self_Dimer_DeltaG":-0.306,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":2,"_row":"533_openPrimeR2017"}
{"Primer":"Opt5_6","Template":"IGHV1-2","Group":"IGHV1-2","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-3.034,"Position_3terminus":1,"All_mismatches":"20,17,16,14,13,11,10,7,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"actttgctccacgctcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":20,"gc_clamp_fw":2,"gc_ratio_fw":0.6,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":60.07,"Self_Dimer_DeltaG":-0.306,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":2,"_row":"534_openPrimeR2017"}
{"Primer":"Opt5_6","Template":"IGHV1-24","Group":"IGHV1-24","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-1.299,"Position_3terminus":2,"All_mismatches":"20,19,18,17,16,12,9,6,3,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"actttgctccacgctcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":20,"gc_clamp_fw":2,"gc_ratio_fw":0.6,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":60.07,"Self_Dimer_DeltaG":-0.306,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":3,"_row":"535_openPrimeR2017"}
{"Primer":"Opt5_6","Template":"IGHV1-3","Group":"IGHV1-3","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-1.514,"Position_3terminus":2,"All_mismatches":"19,18,17,16,14,13,8,7,6,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"actttgctccacgctcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":20,"gc_clamp_fw":2,"gc_ratio_fw":0.6,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":60.07,"Self_Dimer_DeltaG":-0.306,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":2,"_row":"536_openPrimeR2017"}
{"Primer":"Opt5_6","Template":"IGHV1-45","Group":"IGHV1-45","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-1.651,"Position_3terminus":4,"All_mismatches":"19,18,16,15,12,10,8,6,5,4","Worst_Case_MismatchLocal":"3,3,3,3,3,3,3,3,3,3","Primer_Sequence":"actttgctccacgctcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":20,"gc_clamp_fw":2,"gc_ratio_fw":0.6,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":60.07,"Self_Dimer_DeltaG":-0.306,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":3,"Number_of_mismatches_hexamer":3,"_row":"537_openPrimeR2017"}
{"Primer":"Opt5_6","Template":"IGHV1-46","Group":"IGHV1-46","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-1.205,"Position_3terminus":1,"All_mismatches":"20,16,15,12,9,8,7,6,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"actttgctccacgctcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":20,"gc_clamp_fw":2,"gc_ratio_fw":0.6,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":60.07,"Self_Dimer_DeltaG":-0.306,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"538_openPrimeR2017"}
{"Primer":"Opt5_6","Template":"IGHV1-58","Group":"IGHV1-58","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-1.469,"Position_3terminus":1,"All_mismatches":"20,19,18,17,13,12,9,4,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6","Primer_Sequence":"actttgctccacgctcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":20,"gc_clamp_fw":2,"gc_ratio_fw":0.6,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":60.07,"Self_Dimer_DeltaG":-0.306,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":2,"_row":"539_openPrimeR2017"}
{"Primer":"Opt5_6","Template":"IGHV1-69","Group":"IGHV1-69","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-1.862,"Position_3terminus":2,"All_mismatches":"20,19,17,16,15,14,13,8,7,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"actttgctccacgctcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Unamplified","primer_length_fw":20,"gc_clamp_fw":2,"gc_ratio_fw":0.6,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":60.07,"Self_Dimer_DeltaG":-0.306,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":1,"_row":"540_openPrimeR2017"}
{"Primer":"Opt5_6","Template":"IGHV1-69-2","Group":"IGHV1-69-2","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-1.047,"Position_3terminus":2,"All_mismatches":"19,18,15,12,11,9,8,6,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5","Primer_Sequence":"actttgctccacgctcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":20,"gc_clamp_fw":2,"gc_ratio_fw":0.6,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":60.07,"Self_Dimer_DeltaG":-0.306,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":2,"_row":"541_openPrimeR2017"}
{"Primer":"Opt5_6","Template":"IGHV1-8","Group":"IGHV1-8","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-3.945,"Position_3terminus":1,"All_mismatches":"20,17,14,13,11,10,9,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6","Primer_Sequence":"actttgctccacgctcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":20,"gc_clamp_fw":2,"gc_ratio_fw":0.6,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":60.07,"Self_Dimer_DeltaG":-0.306,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":2,"_row":"542_openPrimeR2017"}
{"Primer":"Opt5_6","Template":"IGHV2-26","Group":"IGHV2-26","Number_of_mismatches":2,"primer_efficiency":0.12915,"annealing_DeltaG":-8.977,"Position_3terminus":8,"All_mismatches":"12,8","Worst_Case_MismatchLocal":"0,0","Primer_Sequence":"actttgctccacgctcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":20,"gc_clamp_fw":2,"gc_ratio_fw":0.6,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":60.07,"Self_Dimer_DeltaG":-0.306,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"543_openPrimeR2017"}
{"Primer":"Opt5_6","Template":"IGHV2-5","Group":"IGHV2-5","Number_of_mismatches":0,"primer_efficiency":0.99977,"annealing_DeltaG":-15.903,"All_mismatches":"NA","Worst_Case_MismatchLocal":"0","Primer_Sequence":"actttgctccacgctcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":20,"gc_clamp_fw":2,"gc_ratio_fw":0.6,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":60.07,"Self_Dimer_DeltaG":-0.306,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"544_openPrimeR2017"}
{"Primer":"Opt5_6","Template":"IGHV2-70","Group":"IGHV2-70","Number_of_mismatches":1,"primer_efficiency":0.91118,"annealing_DeltaG":-11.865,"Position_3terminus":14,"All_mismatches":"14","Worst_Case_MismatchLocal":"0","Primer_Sequence":"actttgctccacgctcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":20,"gc_clamp_fw":2,"gc_ratio_fw":0.6,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":60.07,"Self_Dimer_DeltaG":-0.306,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"545_openPrimeR2017"}
{"Primer":"Opt5_6","Template":"IGHV3-11","Group":"IGHV3-11","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-1.456,"Position_3terminus":3,"All_mismatches":"19,17,16,14,13,12,8,7,5,4,3","Worst_Case_MismatchLocal":"4,4,4,4,4,4,4,4,4,4,4","Primer_Sequence":"actttgctccacgctcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":20,"gc_clamp_fw":2,"gc_ratio_fw":0.6,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":60.07,"Self_Dimer_DeltaG":-0.306,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":4,"Number_of_mismatches_hexamer":3,"_row":"546_openPrimeR2017"}
{"Primer":"Opt5_6","Template":"IGHV3-13","Group":"IGHV3-13","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-2.681,"Position_3terminus":1,"All_mismatches":"20,17,15,10,8,7,6,5,4,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"actttgctccacgctcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":20,"gc_clamp_fw":2,"gc_ratio_fw":0.6,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":60.07,"Self_Dimer_DeltaG":-0.306,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":4,"_row":"547_openPrimeR2017"}
{"Primer":"Opt5_6","Template":"IGHV3-15","Group":"IGHV3-15","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-1.784,"Position_3terminus":1,"All_mismatches":"20,17,16,12,11,9,8,7,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"actttgctccacgctcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":20,"gc_clamp_fw":2,"gc_ratio_fw":0.6,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":60.07,"Self_Dimer_DeltaG":-0.306,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":2,"_row":"548_openPrimeR2017"}
{"Primer":"Opt5_6","Template":"IGHV3-20","Group":"IGHV3-20","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-3.539,"Position_3terminus":1,"All_mismatches":"20,19,18,17,15,13,9,3,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6","Primer_Sequence":"actttgctccacgctcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":20,"gc_clamp_fw":2,"gc_ratio_fw":0.6,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":60.07,"Self_Dimer_DeltaG":-0.306,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":2,"_row":"549_openPrimeR2017"}
{"Primer":"Opt5_6","Template":"IGHV3-21","Group":"IGHV3-21","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-0.799,"Position_3terminus":1,"All_mismatches":"19,18,17,15,13,9,7,6,5,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"actttgctccacgctcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":20,"gc_clamp_fw":2,"gc_ratio_fw":0.6,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":60.07,"Self_Dimer_DeltaG":-0.306,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"550_openPrimeR2017"}
{"Primer":"Opt5_6","Template":"IGHV3-23","Group":"IGHV3-23","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-1.456,"Position_3terminus":3,"All_mismatches":"19,17,16,14,13,12,8,7,5,4,3","Worst_Case_MismatchLocal":"4,4,4,4,4,4,4,4,4,4,4","Primer_Sequence":"actttgctccacgctcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":20,"gc_clamp_fw":2,"gc_ratio_fw":0.6,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":60.07,"Self_Dimer_DeltaG":-0.306,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":4,"Number_of_mismatches_hexamer":3,"_row":"551_openPrimeR2017"}
{"Primer":"Opt5_6","Template":"IGHV3-30","Group":"IGHV3-30","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-2.582,"Position_3terminus":1,"All_mismatches":"20,19,15,11,10,9,4,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6","Primer_Sequence":"actttgctccacgctcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":20,"gc_clamp_fw":2,"gc_ratio_fw":0.6,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":60.07,"Self_Dimer_DeltaG":-0.306,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"552_openPrimeR2017"}
{"Primer":"Opt5_6","Template":"IGHV3-30-3","Group":"IGHV3-30-3","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-2.582,"Position_3terminus":1,"All_mismatches":"20,19,15,11,10,9,4,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6","Primer_Sequence":"actttgctccacgctcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":20,"gc_clamp_fw":2,"gc_ratio_fw":0.6,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":60.07,"Self_Dimer_DeltaG":-0.306,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"553_openPrimeR2017"}
{"Primer":"Opt5_6","Template":"IGHV3-33","Group":"IGHV3-33","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-2.582,"Position_3terminus":1,"All_mismatches":"20,19,15,11,10,9,4,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6","Primer_Sequence":"actttgctccacgctcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":20,"gc_clamp_fw":2,"gc_ratio_fw":0.6,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":60.07,"Self_Dimer_DeltaG":-0.306,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"554_openPrimeR2017"}
{"Primer":"Opt5_6","Template":"IGHV3-43","Group":"IGHV3-43","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-1.784,"Position_3terminus":1,"All_mismatches":"17,16,12,11,10,9,8,7,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"actttgctccacgctcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":20,"gc_clamp_fw":2,"gc_ratio_fw":0.6,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":60.07,"Self_Dimer_DeltaG":-0.306,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":2,"_row":"555_openPrimeR2017"}
{"Primer":"Opt5_6","Template":"IGHV3-48","Group":"IGHV3-48","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-1.934,"Position_3terminus":1,"All_mismatches":"20,17,12,11,10,9,8,7,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"actttgctccacgctcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":20,"gc_clamp_fw":2,"gc_ratio_fw":0.6,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":60.07,"Self_Dimer_DeltaG":-0.306,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":2,"_row":"556_openPrimeR2017"}
{"Primer":"Opt5_6","Template":"IGHV3-49","Group":"IGHV3-49","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-1.784,"Position_3terminus":1,"All_mismatches":"20,16,12,11,10,9,8,7,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"actttgctccacgctcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":20,"gc_clamp_fw":2,"gc_ratio_fw":0.6,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":60.07,"Self_Dimer_DeltaG":-0.306,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":2,"_row":"557_openPrimeR2017"}
{"Primer":"Opt5_6","Template":"IGHV3-53","Group":"IGHV3-53","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-2.197,"Position_3terminus":2,"All_mismatches":"20,19,15,12,11,10,9,5,4,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"actttgctccacgctcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":20,"gc_clamp_fw":2,"gc_ratio_fw":0.6,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":60.07,"Self_Dimer_DeltaG":-0.306,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":3,"_row":"558_openPrimeR2017"}
{"Primer":"Opt5_6","Template":"IGHV3-64","Group":"IGHV3-64","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-1.456,"Position_3terminus":3,"All_mismatches":"19,17,16,14,13,12,8,7,5,4,3","Worst_Case_MismatchLocal":"4,4,4,4,4,4,4,4,4,4,4","Primer_Sequence":"actttgctccacgctcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":20,"gc_clamp_fw":2,"gc_ratio_fw":0.6,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":60.07,"Self_Dimer_DeltaG":-0.306,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":4,"Number_of_mismatches_hexamer":3,"_row":"559_openPrimeR2017"}
{"Primer":"Opt5_6","Template":"IGHV3-64D","Group":"IGHV3-64D","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-3.252,"Position_3terminus":1,"All_mismatches":"20,17,16,12,11,10,9,8,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"actttgctccacgctcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":20,"gc_clamp_fw":2,"gc_ratio_fw":0.6,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":60.07,"Self_Dimer_DeltaG":-0.306,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":2,"_row":"560_openPrimeR2017"}
{"Primer":"Opt5_6","Template":"IGHV3-66","Group":"IGHV3-66","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-1.461,"Position_3terminus":1,"All_mismatches":"20,19,18,14,9,8,5,4,3,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"actttgctccacgctcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":20,"gc_clamp_fw":2,"gc_ratio_fw":0.6,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":60.07,"Self_Dimer_DeltaG":-0.306,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":4,"_row":"561_openPrimeR2017"}
{"Primer":"Opt5_6","Template":"IGHV3-7","Group":"IGHV3-7","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-1.876,"Position_3terminus":2,"All_mismatches":"20,17,16,15,9,7,6,4,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5","Primer_Sequence":"actttgctccacgctcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":20,"gc_clamp_fw":2,"gc_ratio_fw":0.6,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":60.07,"Self_Dimer_DeltaG":-0.306,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":3,"_row":"562_openPrimeR2017"}
{"Primer":"Opt5_6","Template":"IGHV3-72","Group":"IGHV3-72","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-3.427,"Position_3terminus":1,"All_mismatches":"20,19,18,17,15,13,9,3,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6","Primer_Sequence":"actttgctccacgctcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":20,"gc_clamp_fw":2,"gc_ratio_fw":0.6,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":60.07,"Self_Dimer_DeltaG":-0.306,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":2,"_row":"563_openPrimeR2017"}
{"Primer":"Opt5_6","Template":"IGHV3-73","Group":"IGHV3-73","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-1.461,"Position_3terminus":1,"All_mismatches":"20,19,18,14,9,8,5,4,3,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"actttgctccacgctcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":20,"gc_clamp_fw":2,"gc_ratio_fw":0.6,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":60.07,"Self_Dimer_DeltaG":-0.306,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":4,"_row":"564_openPrimeR2017"}
{"Primer":"Opt5_6","Template":"IGHV3-74","Group":"IGHV3-74","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-2.325,"Position_3terminus":5,"All_mismatches":"20,19,18,12,11,10,8,7,6,5","Worst_Case_MismatchLocal":"2,2,2,2,2,2,2,2,2,2","Primer_Sequence":"actttgctccacgctcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":20,"gc_clamp_fw":2,"gc_ratio_fw":0.6,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":60.07,"Self_Dimer_DeltaG":-0.306,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":2,"Number_of_mismatches_hexamer":2,"_row":"565_openPrimeR2017"}
{"Primer":"Opt5_6","Template":"IGHV3-9","Group":"IGHV3-9","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-1.784,"Position_3terminus":1,"All_mismatches":"17,16,12,11,9,8,7,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6","Primer_Sequence":"actttgctccacgctcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":20,"gc_clamp_fw":2,"gc_ratio_fw":0.6,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":60.07,"Self_Dimer_DeltaG":-0.306,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":2,"_row":"566_openPrimeR2017"}
{"Primer":"Opt5_6","Template":"IGHV4-28","Group":"IGHV4-28","Number_of_mismatches":7,"primer_efficiency":0.00013,"annealing_DeltaG":-4.212,"Position_3terminus":8,"All_mismatches":"20,17,14,12,10,9,8","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0","Primer_Sequence":"actttgctccacgctcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Unamplified","primer_length_fw":20,"gc_clamp_fw":2,"gc_ratio_fw":0.6,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":60.07,"Self_Dimer_DeltaG":-0.306,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"567_openPrimeR2017"}
{"Primer":"Opt5_6","Template":"IGHV4-30-2","Group":"IGHV4-30-2","Number_of_mismatches":7,"primer_efficiency":0.00013,"annealing_DeltaG":-4.212,"Position_3terminus":8,"All_mismatches":"20,17,14,12,10,9,8","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0","Primer_Sequence":"actttgctccacgctcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Unamplified","primer_length_fw":20,"gc_clamp_fw":2,"gc_ratio_fw":0.6,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":60.07,"Self_Dimer_DeltaG":-0.306,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"568_openPrimeR2017"}
{"Primer":"Opt5_6","Template":"IGHV4-31","Group":"IGHV4-31","Number_of_mismatches":7,"primer_efficiency":0.00013,"annealing_DeltaG":-4.212,"Position_3terminus":8,"All_mismatches":"20,17,14,12,10,9,8","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0","Primer_Sequence":"actttgctccacgctcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Unamplified","primer_length_fw":20,"gc_clamp_fw":2,"gc_ratio_fw":0.6,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":60.07,"Self_Dimer_DeltaG":-0.306,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"569_openPrimeR2017"}
{"Primer":"Opt5_6","Template":"IGHV4-34","Group":"IGHV4-34","Number_of_mismatches":8,"primer_efficiency":4.54138e-06,"annealing_DeltaG":-2.681,"Position_3terminus":2,"All_mismatches":"20,17,14,12,10,9,8,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5","Primer_Sequence":"actttgctccacgctcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Amplified","primer_length_fw":20,"gc_clamp_fw":2,"gc_ratio_fw":0.6,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":60.07,"Self_Dimer_DeltaG":-0.306,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":1,"_row":"570_openPrimeR2017"}
{"Primer":"Opt5_6","Template":"IGHV4-38-2","Group":"IGHV4-38-2","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-1.989,"Position_3terminus":1,"All_mismatches":"17,16,15,13,12,11,9,6,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6","Primer_Sequence":"actttgctccacgctcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":20,"gc_clamp_fw":2,"gc_ratio_fw":0.6,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":60.07,"Self_Dimer_DeltaG":-0.306,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":2,"_row":"571_openPrimeR2017"}
{"Primer":"Opt5_6","Template":"IGHV4-39","Group":"IGHV4-39","Number_of_mismatches":7,"primer_efficiency":0.00013,"annealing_DeltaG":-4.212,"Position_3terminus":8,"All_mismatches":"20,17,14,12,10,9,8","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0","Primer_Sequence":"actttgctccacgctcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Unamplified","primer_length_fw":20,"gc_clamp_fw":2,"gc_ratio_fw":0.6,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":60.07,"Self_Dimer_DeltaG":-0.306,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"572_openPrimeR2017"}
{"Primer":"Opt5_6","Template":"IGHV4-4","Group":"IGHV4-4","Number_of_mismatches":7,"primer_efficiency":0.00013,"annealing_DeltaG":-4.212,"Position_3terminus":8,"All_mismatches":"20,17,14,12,10,9,8","Worst_Case_MismatchLocal":"0,0,0,0,0,0,0","Primer_Sequence":"actttgctccacgctcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":20,"gc_clamp_fw":2,"gc_ratio_fw":0.6,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":60.07,"Self_Dimer_DeltaG":-0.306,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"573_openPrimeR2017"}
{"Primer":"Opt5_6","Template":"IGHV4-59","Group":"IGHV4-59","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-2.126,"Position_3terminus":2,"All_mismatches":"20,17,14,12,10,9,8,5,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5","Primer_Sequence":"actttgctccacgctcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":20,"gc_clamp_fw":2,"gc_ratio_fw":0.6,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":60.07,"Self_Dimer_DeltaG":-0.306,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":2,"_row":"574_openPrimeR2017"}
{"Primer":"Opt5_6","Template":"IGHV4-61","Group":"IGHV4-61","Number_of_mismatches":8,"primer_efficiency":4.54138e-06,"annealing_DeltaG":-2.681,"Position_3terminus":2,"All_mismatches":"20,17,14,12,10,9,8,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5","Primer_Sequence":"actttgctccacgctcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Unamplified","primer_length_fw":20,"gc_clamp_fw":2,"gc_ratio_fw":0.6,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":60.07,"Self_Dimer_DeltaG":-0.306,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":1,"_row":"575_openPrimeR2017"}
{"Primer":"Opt5_6","Template":"IGHV5-10-1","Group":"IGHV5-10-1","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-2.681,"Position_3terminus":1,"All_mismatches":"20,17,15,10,8,7,6,5,4,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"actttgctccacgctcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":20,"gc_clamp_fw":2,"gc_ratio_fw":0.6,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":60.07,"Self_Dimer_DeltaG":-0.306,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":4,"_row":"576_openPrimeR2017"}
{"Primer":"Opt5_6","Template":"IGHV5-51","Group":"IGHV5-51","Number_of_mismatches":7,"primer_efficiency":0,"annealing_DeltaG":-3.016,"Position_3terminus":1,"All_mismatches":"20,17,15,8,7,4,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6","Primer_Sequence":"actttgctccacgctcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":20,"gc_clamp_fw":2,"gc_ratio_fw":0.6,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":60.07,"Self_Dimer_DeltaG":-0.306,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":2,"_row":"577_openPrimeR2017"}
{"Primer":"Opt5_6","Template":"IGHV6-1","Group":"IGHV6-1","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-3.618,"Position_3terminus":4,"All_mismatches":"20,17,16,15,12,10,9,6,4","Worst_Case_MismatchLocal":"3,3,3,3,3,3,3,3,3","Primer_Sequence":"actttgctccacgctcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":20,"gc_clamp_fw":2,"gc_ratio_fw":0.6,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":60.07,"Self_Dimer_DeltaG":-0.306,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":3,"Number_of_mismatches_hexamer":2,"_row":"578_openPrimeR2017"}
{"Primer":"Opt5_6","Template":"IGHV7-4-1","Group":"IGHV7-4-1","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-4.102,"Position_3terminus":2,"All_mismatches":"20,18,17,16,15,14,13,8,6,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"actttgctccacgctcctgc","Terminal_Dinucleotide":"gc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":20,"gc_clamp_fw":2,"gc_ratio_fw":0.6,"no_runs_fw":3,"no_repeats_fw":1,"melting_temp":60.07,"Self_Dimer_DeltaG":-0.306,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":2,"_row":"579_openPrimeR2017"}
{"Primer":"Opt5_7","Template":"IGHV1-18","Group":"IGHV1-18","Number_of_mismatches":0,"primer_efficiency":0.99966,"annealing_DeltaG":-15.367,"All_mismatches":"NA","Worst_Case_MismatchLocal":"0","Primer_Sequence":"atggactggacctggagcatc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":21,"gc_clamp_fw":1,"gc_ratio_fw":0.57143,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":58.3,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"580_openPrimeR2017"}
{"Primer":"Opt5_7","Template":"IGHV1-2","Group":"IGHV1-2","Number_of_mismatches":1,"primer_efficiency":0.29129,"annealing_DeltaG":-12.039,"Position_3terminus":4,"All_mismatches":"4","Worst_Case_MismatchLocal":"3","Primer_Sequence":"atggactggacctggagcatc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Amplified","primer_length_fw":21,"gc_clamp_fw":1,"gc_ratio_fw":0.57143,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":58.3,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":3,"Number_of_mismatches_hexamer":1,"_row":"581_openPrimeR2017"}
{"Primer":"Opt5_7","Template":"IGHV1-24","Group":"IGHV1-24","Number_of_mismatches":2,"primer_efficiency":0.14302,"annealing_DeltaG":-10.082,"Position_3terminus":4,"All_mismatches":"13,4","Worst_Case_MismatchLocal":"3,3","Primer_Sequence":"atggactggacctggagcatc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Amplified","primer_length_fw":21,"gc_clamp_fw":1,"gc_ratio_fw":0.57143,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":58.3,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":3,"Number_of_mismatches_hexamer":1,"_row":"582_openPrimeR2017"}
{"Primer":"Opt5_7","Template":"IGHV1-3","Group":"IGHV1-3","Number_of_mismatches":1,"primer_efficiency":0.29129,"annealing_DeltaG":-12.039,"Position_3terminus":4,"All_mismatches":"4","Worst_Case_MismatchLocal":"3","Primer_Sequence":"atggactggacctggagcatc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Amplified","primer_length_fw":21,"gc_clamp_fw":1,"gc_ratio_fw":0.57143,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":58.3,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":3,"Number_of_mismatches_hexamer":1,"_row":"583_openPrimeR2017"}
{"Primer":"Opt5_7","Template":"IGHV1-45","Group":"IGHV1-45","Number_of_mismatches":1,"primer_efficiency":0.29367,"annealing_DeltaG":-12.126,"Position_3terminus":4,"All_mismatches":"4","Worst_Case_MismatchLocal":"3","Primer_Sequence":"atggactggacctggagcatc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Amplified","primer_length_fw":21,"gc_clamp_fw":1,"gc_ratio_fw":0.57143,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":58.3,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":3,"Number_of_mismatches_hexamer":1,"_row":"584_openPrimeR2017"}
{"Primer":"Opt5_7","Template":"IGHV1-46","Group":"IGHV1-46","Number_of_mismatches":2,"primer_efficiency":0,"annealing_DeltaG":-12.039,"Position_3terminus":3,"All_mismatches":"4,3","Worst_Case_MismatchLocal":"4,4","Primer_Sequence":"atggactggacctggagcatc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Amplified","primer_length_fw":21,"gc_clamp_fw":1,"gc_ratio_fw":0.57143,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":58.3,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":4,"Number_of_mismatches_hexamer":2,"_row":"585_openPrimeR2017"}
{"Primer":"Opt5_7","Template":"IGHV1-58","Group":"IGHV1-58","Number_of_mismatches":3,"primer_efficiency":0.00078,"annealing_DeltaG":-6.165,"Position_3terminus":4,"All_mismatches":"11,10,4","Worst_Case_MismatchLocal":"3,3,3","Primer_Sequence":"atggactggacctggagcatc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Amplified","primer_length_fw":21,"gc_clamp_fw":1,"gc_ratio_fw":0.57143,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":58.3,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":3,"Number_of_mismatches_hexamer":1,"_row":"586_openPrimeR2017"}
{"Primer":"Opt5_7","Template":"IGHV1-69","Group":"IGHV1-69","Number_of_mismatches":2,"primer_efficiency":0,"annealing_DeltaG":-12.039,"Position_3terminus":3,"All_mismatches":"4,3","Worst_Case_MismatchLocal":"4,4","Primer_Sequence":"atggactggacctggagcatc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Amplified","primer_length_fw":21,"gc_clamp_fw":1,"gc_ratio_fw":0.57143,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":58.3,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":4,"Number_of_mismatches_hexamer":2,"_row":"587_openPrimeR2017"}
{"Primer":"Opt5_7","Template":"IGHV1-69-2","Group":"IGHV1-69-2","Number_of_mismatches":2,"primer_efficiency":0.14302,"annealing_DeltaG":-10.082,"Position_3terminus":4,"All_mismatches":"13,4","Worst_Case_MismatchLocal":"3,3","Primer_Sequence":"atggactggacctggagcatc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Amplified","primer_length_fw":21,"gc_clamp_fw":1,"gc_ratio_fw":0.57143,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":58.3,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":3,"Number_of_mismatches_hexamer":1,"_row":"588_openPrimeR2017"}
{"Primer":"Opt5_7","Template":"IGHV1-8","Group":"IGHV1-8","Number_of_mismatches":1,"primer_efficiency":0.29129,"annealing_DeltaG":-12.039,"Position_3terminus":4,"All_mismatches":"4","Worst_Case_MismatchLocal":"3","Primer_Sequence":"atggactggacctggagcatc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Amplified","primer_length_fw":21,"gc_clamp_fw":1,"gc_ratio_fw":0.57143,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":58.3,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":3,"Number_of_mismatches_hexamer":1,"_row":"589_openPrimeR2017"}
{"Primer":"Opt5_7","Template":"IGHV2-26","Group":"IGHV2-26","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-3.658,"Position_3terminus":2,"All_mismatches":"21,17,15,14,12,11,6,4,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5","Primer_Sequence":"atggactggacctggagcatc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Amplified","primer_length_fw":21,"gc_clamp_fw":1,"gc_ratio_fw":0.57143,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":58.3,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":3,"_row":"590_openPrimeR2017"}
{"Primer":"Opt5_7","Template":"IGHV2-5","Group":"IGHV2-5","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-1.315,"Position_3terminus":2,"All_mismatches":"21,20,17,14,12,9,8,5,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5","Primer_Sequence":"atggactggacctggagcatc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":1,"gc_ratio_fw":0.57143,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":58.3,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":2,"_row":"591_openPrimeR2017"}
{"Primer":"Opt5_7","Template":"IGHV2-70","Group":"IGHV2-70","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-1.695,"Position_3terminus":1,"All_mismatches":"20,18,16,15,13,10,6,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6","Primer_Sequence":"atggactggacctggagcatc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Amplified","primer_length_fw":21,"gc_clamp_fw":1,"gc_ratio_fw":0.57143,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":58.3,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"592_openPrimeR2017"}
{"Primer":"Opt5_7","Template":"IGHV3-11","Group":"IGHV3-11","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-2.72,"Position_3terminus":1,"All_mismatches":"21,20,14,13,12,10,4,3,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6","Primer_Sequence":"atggactggacctggagcatc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":1,"gc_ratio_fw":0.57143,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":58.3,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"593_openPrimeR2017"}
{"Primer":"Opt5_7","Template":"IGHV3-13","Group":"IGHV3-13","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-2.314,"Position_3terminus":1,"All_mismatches":"16,14,12,11,10,9,8,3,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"atggactggacctggagcatc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":1,"gc_ratio_fw":0.57143,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":58.3,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"594_openPrimeR2017"}
{"Primer":"Opt5_7","Template":"IGHV3-15","Group":"IGHV3-15","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-2.72,"Position_3terminus":1,"All_mismatches":"21,20,14,13,12,10,4,3,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6","Primer_Sequence":"atggactggacctggagcatc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":1,"gc_ratio_fw":0.57143,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":58.3,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"595_openPrimeR2017"}
{"Primer":"Opt5_7","Template":"IGHV3-20","Group":"IGHV3-20","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-2.178,"Position_3terminus":1,"All_mismatches":"20,19,18,16,14,12,10,4,3,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"atggactggacctggagcatc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":1,"gc_ratio_fw":0.57143,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":58.3,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"596_openPrimeR2017"}
{"Primer":"Opt5_7","Template":"IGHV3-21","Group":"IGHV3-21","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-2.506,"Position_3terminus":1,"All_mismatches":"21,20,18,13,12,10,9,8,4,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"atggactggacctggagcatc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":1,"gc_ratio_fw":0.57143,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":58.3,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":2,"_row":"597_openPrimeR2017"}
{"Primer":"Opt5_7","Template":"IGHV3-23","Group":"IGHV3-23","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-3.072,"Position_3terminus":1,"All_mismatches":"21,20,13,10,9,8,4,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6","Primer_Sequence":"atggactggacctggagcatc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":1,"gc_ratio_fw":0.57143,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":58.3,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"598_openPrimeR2017"}
{"Primer":"Opt5_7","Template":"IGHV3-30","Group":"IGHV3-30","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-2.72,"Position_3terminus":1,"All_mismatches":"21,20,14,13,12,10,4,3,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6","Primer_Sequence":"atggactggacctggagcatc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":1,"gc_ratio_fw":0.57143,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":58.3,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"599_openPrimeR2017"}
{"Primer":"Opt5_7","Template":"IGHV3-30-3","Group":"IGHV3-30-3","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-2.72,"Position_3terminus":1,"All_mismatches":"21,20,14,13,12,10,4,3,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6","Primer_Sequence":"atggactggacctggagcatc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":1,"gc_ratio_fw":0.57143,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":58.3,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"600_openPrimeR2017"}
{"Primer":"Opt5_7","Template":"IGHV3-33","Group":"IGHV3-33","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-2.72,"Position_3terminus":1,"All_mismatches":"21,20,14,13,12,10,4,3,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6","Primer_Sequence":"atggactggacctggagcatc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":1,"gc_ratio_fw":0.57143,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":58.3,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"601_openPrimeR2017"}
{"Primer":"Opt5_7","Template":"IGHV3-43","Group":"IGHV3-43","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-2.705,"Position_3terminus":1,"All_mismatches":"21,18,16,13,11,6,4,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6","Primer_Sequence":"atggactggacctggagcatc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":1,"gc_ratio_fw":0.57143,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":58.3,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":4,"_row":"602_openPrimeR2017"}
{"Primer":"Opt5_7","Template":"IGHV3-48","Group":"IGHV3-48","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-2.232,"Position_3terminus":1,"All_mismatches":"21,20,18,13,10,9,8,4,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"atggactggacctggagcatc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":1,"gc_ratio_fw":0.57143,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":58.3,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"603_openPrimeR2017"}
{"Primer":"Opt5_7","Template":"IGHV3-49","Group":"IGHV3-49","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-2.027,"Position_3terminus":2,"All_mismatches":"21,20,18,13,10,9,8,5,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5","Primer_Sequence":"atggactggacctggagcatc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":1,"gc_ratio_fw":0.57143,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":58.3,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":2,"_row":"604_openPrimeR2017"}
{"Primer":"Opt5_7","Template":"IGHV3-53","Group":"IGHV3-53","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-2.72,"Position_3terminus":1,"All_mismatches":"21,20,14,13,12,10,4,3,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6","Primer_Sequence":"atggactggacctggagcatc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":1,"gc_ratio_fw":0.57143,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":58.3,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"605_openPrimeR2017"}
{"Primer":"Opt5_7","Template":"IGHV3-64","Group":"IGHV3-64","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-2.705,"Position_3terminus":1,"All_mismatches":"21,18,17,16,13,11,6,4,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"atggactggacctggagcatc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":1,"gc_ratio_fw":0.57143,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":58.3,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":4,"_row":"606_openPrimeR2017"}
{"Primer":"Opt5_7","Template":"IGHV3-64D","Group":"IGHV3-64D","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-1.695,"Position_3terminus":2,"All_mismatches":"20,18,16,15,13,10,6,4,3,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"atggactggacctggagcatc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":1,"gc_ratio_fw":0.57143,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":58.3,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":4,"_row":"607_openPrimeR2017"}
{"Primer":"Opt5_7","Template":"IGHV3-66","Group":"IGHV3-66","Number_of_mismatches":8,"primer_efficiency":0,"annealing_DeltaG":-2.72,"Position_3terminus":1,"All_mismatches":"21,20,14,12,10,4,3,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6","Primer_Sequence":"atggactggacctggagcatc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":1,"gc_ratio_fw":0.57143,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":58.3,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"608_openPrimeR2017"}
{"Primer":"Opt5_7","Template":"IGHV3-7","Group":"IGHV3-7","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-2.314,"Position_3terminus":1,"All_mismatches":"16,14,12,11,10,9,8,3,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"atggactggacctggagcatc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":1,"gc_ratio_fw":0.57143,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":58.3,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"609_openPrimeR2017"}
{"Primer":"Opt5_7","Template":"IGHV3-72","Group":"IGHV3-72","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-2.72,"Position_3terminus":1,"All_mismatches":"21,20,14,13,12,10,4,3,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6","Primer_Sequence":"atggactggacctggagcatc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":1,"gc_ratio_fw":0.57143,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":58.3,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"610_openPrimeR2017"}
{"Primer":"Opt5_7","Template":"IGHV3-73","Group":"IGHV3-73","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-6.369,"Position_3terminus":1,"All_mismatches":"11,10,9,8,6,5,4,3,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"atggactggacctggagcatc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":6,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":1,"gc_ratio_fw":0.57143,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":58.3,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":6,"_row":"611_openPrimeR2017"}
{"Primer":"Opt5_7","Template":"IGHV3-74","Group":"IGHV3-74","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-2.94,"Position_3terminus":2,"All_mismatches":"20,18,16,15,13,10,6,4,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5","Primer_Sequence":"atggactggacctggagcatc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":1,"gc_ratio_fw":0.57143,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":58.3,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":3,"_row":"612_openPrimeR2017"}
{"Primer":"Opt5_7","Template":"IGHV3-9","Group":"IGHV3-9","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-3.125,"Position_3terminus":3,"All_mismatches":"21,17,16,15,14,11,5,4,3","Worst_Case_MismatchLocal":"4,4,4,4,4,4,4,4,4","Primer_Sequence":"atggactggacctggagcatc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":1,"gc_ratio_fw":0.57143,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":58.3,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":4,"Number_of_mismatches_hexamer":3,"_row":"613_openPrimeR2017"}
{"Primer":"Opt5_7","Template":"IGHV4-28","Group":"IGHV4-28","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-2.712,"Position_3terminus":2,"All_mismatches":"21,20,18,17,13,12,9,6,5,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"atggactggacctggagcatc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":1,"gc_ratio_fw":0.57143,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":58.3,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":3,"_row":"614_openPrimeR2017"}
{"Primer":"Opt5_7","Template":"IGHV4-30-2","Group":"IGHV4-30-2","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-2.608,"Position_3terminus":1,"All_mismatches":"20,18,16,15,13,10,6,4,3,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"atggactggacctggagcatc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":1,"gc_ratio_fw":0.57143,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":58.3,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":4,"_row":"615_openPrimeR2017"}
{"Primer":"Opt5_7","Template":"IGHV4-31","Group":"IGHV4-31","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-2.712,"Position_3terminus":2,"All_mismatches":"21,20,18,17,13,12,9,6,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5","Primer_Sequence":"atggactggacctggagcatc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Amplified","primer_length_fw":21,"gc_clamp_fw":1,"gc_ratio_fw":0.57143,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":58.3,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":2,"_row":"616_openPrimeR2017"}
{"Primer":"Opt5_7","Template":"IGHV4-34","Group":"IGHV4-34","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-2.712,"Position_3terminus":2,"All_mismatches":"21,20,18,17,13,12,9,6,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5","Primer_Sequence":"atggactggacctggagcatc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Amplified","primer_length_fw":21,"gc_clamp_fw":1,"gc_ratio_fw":0.57143,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":58.3,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":2,"_row":"617_openPrimeR2017"}
{"Primer":"Opt5_7","Template":"IGHV4-38-2","Group":"IGHV4-38-2","Number_of_mismatches":8,"primer_efficiency":0,"annealing_DeltaG":-2.72,"Position_3terminus":2,"All_mismatches":"21,20,14,13,12,10,7,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5","Primer_Sequence":"atggactggacctggagcatc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Amplified","primer_length_fw":21,"gc_clamp_fw":1,"gc_ratio_fw":0.57143,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":58.3,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":1,"_row":"618_openPrimeR2017"}
{"Primer":"Opt5_7","Template":"IGHV4-39","Group":"IGHV4-39","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-1.695,"Position_3terminus":1,"All_mismatches":"20,18,16,15,13,10,6,4,3,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"atggactggacctggagcatc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":1,"gc_ratio_fw":0.57143,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":58.3,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":4,"_row":"619_openPrimeR2017"}
{"Primer":"Opt5_7","Template":"IGHV4-4","Group":"IGHV4-4","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-2.712,"Position_3terminus":2,"All_mismatches":"21,20,18,17,13,12,9,6,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5","Primer_Sequence":"atggactggacctggagcatc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Amplified","primer_length_fw":21,"gc_clamp_fw":1,"gc_ratio_fw":0.57143,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":58.3,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":2,"_row":"620_openPrimeR2017"}
{"Primer":"Opt5_7","Template":"IGHV4-59","Group":"IGHV4-59","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-2.712,"Position_3terminus":2,"All_mismatches":"21,20,18,17,13,12,9,6,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5","Primer_Sequence":"atggactggacctggagcatc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":1,"gc_ratio_fw":0.57143,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":58.3,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":2,"_row":"621_openPrimeR2017"}
{"Primer":"Opt5_7","Template":"IGHV4-61","Group":"IGHV4-61","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-2.712,"Position_3terminus":2,"All_mismatches":"21,20,18,17,13,12,9,6,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5","Primer_Sequence":"atggactggacctggagcatc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":1,"gc_ratio_fw":0.57143,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":58.3,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":2,"_row":"622_openPrimeR2017"}
{"Primer":"Opt5_7","Template":"IGHV5-10-1","Group":"IGHV5-10-1","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-2.093,"Position_3terminus":3,"All_mismatches":"17,16,14,13,9,8,7,5,3","Worst_Case_MismatchLocal":"4,4,4,4,4,4,4,4,4","Primer_Sequence":"atggactggacctggagcatc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":1,"gc_ratio_fw":0.57143,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":58.3,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":4,"Number_of_mismatches_hexamer":2,"_row":"623_openPrimeR2017"}
{"Primer":"Opt5_7","Template":"IGHV5-51","Group":"IGHV5-51","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-2.093,"Position_3terminus":3,"All_mismatches":"17,16,14,13,9,8,7,5,3","Worst_Case_MismatchLocal":"4,4,4,4,4,4,4,4,4","Primer_Sequence":"atggactggacctggagcatc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":1,"gc_ratio_fw":0.57143,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":58.3,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":4,"Number_of_mismatches_hexamer":2,"_row":"624_openPrimeR2017"}
{"Primer":"Opt5_7","Template":"IGHV6-1","Group":"IGHV6-1","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-2.004,"Position_3terminus":1,"All_mismatches":"17,16,15,13,12,7,6,4,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6","Primer_Sequence":"atggactggacctggagcatc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":1,"gc_ratio_fw":0.57143,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":58.3,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"625_openPrimeR2017"}
{"Primer":"Opt5_7","Template":"IGHV7-4-1","Group":"IGHV7-4-1","Number_of_mismatches":1,"primer_efficiency":0.29129,"annealing_DeltaG":-12.039,"Position_3terminus":4,"All_mismatches":"4","Worst_Case_MismatchLocal":"3","Primer_Sequence":"atggactggacctggagcatc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Amplified","primer_length_fw":21,"gc_clamp_fw":1,"gc_ratio_fw":0.57143,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":58.3,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":3,"Number_of_mismatches_hexamer":1,"_row":"626_openPrimeR2017"}
{"Primer":"Opt5_8","Template":"IGHV1-18","Group":"IGHV1-18","Number_of_mismatches":2,"primer_efficiency":0,"annealing_DeltaG":-12.218,"Position_3terminus":4,"All_mismatches":"5,4","Worst_Case_MismatchLocal":"3,3","Primer_Sequence":"atggactggacctggaggttcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Amplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":60.38,"Self_Dimer_DeltaG":-2.11,"Structure_deltaG_fw":-1.86,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":3,"Number_of_mismatches_hexamer":2,"_row":"627_openPrimeR2017"}
{"Primer":"Opt5_8","Template":"IGHV1-2","Group":"IGHV1-2","Number_of_mismatches":1,"primer_efficiency":0.30248,"annealing_DeltaG":-13.283,"Position_3terminus":4,"All_mismatches":"4","Worst_Case_MismatchLocal":"3","Primer_Sequence":"atggactggacctggaggttcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Amplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":60.38,"Self_Dimer_DeltaG":-2.11,"Structure_deltaG_fw":-1.86,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":3,"Number_of_mismatches_hexamer":1,"_row":"628_openPrimeR2017"}
{"Primer":"Opt5_8","Template":"IGHV1-24","Group":"IGHV1-24","Number_of_mismatches":2,"primer_efficiency":0.22785,"annealing_DeltaG":-11.326,"Position_3terminus":4,"All_mismatches":"14,4","Worst_Case_MismatchLocal":"3,3","Primer_Sequence":"atggactggacctggaggttcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Amplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":60.38,"Self_Dimer_DeltaG":-2.11,"Structure_deltaG_fw":-1.86,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":3,"Number_of_mismatches_hexamer":1,"_row":"629_openPrimeR2017"}
{"Primer":"Opt5_8","Template":"IGHV1-3","Group":"IGHV1-3","Number_of_mismatches":1,"primer_efficiency":0.30248,"annealing_DeltaG":-13.283,"Position_3terminus":4,"All_mismatches":"4","Worst_Case_MismatchLocal":"3","Primer_Sequence":"atggactggacctggaggttcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Amplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":60.38,"Self_Dimer_DeltaG":-2.11,"Structure_deltaG_fw":-1.86,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":3,"Number_of_mismatches_hexamer":1,"_row":"630_openPrimeR2017"}
{"Primer":"Opt5_8","Template":"IGHV1-45","Group":"IGHV1-45","Number_of_mismatches":2,"primer_efficiency":0,"annealing_DeltaG":-12.133,"Position_3terminus":4,"All_mismatches":"5,4","Worst_Case_MismatchLocal":"3,3","Primer_Sequence":"atggactggacctggaggttcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Amplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":60.38,"Self_Dimer_DeltaG":-2.11,"Structure_deltaG_fw":-1.86,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":3,"Number_of_mismatches_hexamer":2,"_row":"631_openPrimeR2017"}
{"Primer":"Opt5_8","Template":"IGHV1-46","Group":"IGHV1-46","Number_of_mismatches":2,"primer_efficiency":0,"annealing_DeltaG":-13.196,"Position_3terminus":1,"All_mismatches":"4,1","Worst_Case_MismatchLocal":"6,6","Primer_Sequence":"atggactggacctggaggttcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Amplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":60.38,"Self_Dimer_DeltaG":-2.11,"Structure_deltaG_fw":-1.86,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":2,"_row":"632_openPrimeR2017"}
{"Primer":"Opt5_8","Template":"IGHV1-58","Group":"IGHV1-58","Number_of_mismatches":3,"primer_efficiency":0.00214,"annealing_DeltaG":-7.409,"Position_3terminus":4,"All_mismatches":"12,11,4","Worst_Case_MismatchLocal":"3,3,3","Primer_Sequence":"atggactggacctggaggttcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Amplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":60.38,"Self_Dimer_DeltaG":-2.11,"Structure_deltaG_fw":-1.86,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":3,"Number_of_mismatches_hexamer":1,"_row":"633_openPrimeR2017"}
{"Primer":"Opt5_8","Template":"IGHV1-69","Group":"IGHV1-69","Number_of_mismatches":0,"primer_efficiency":0.99966,"annealing_DeltaG":-16.179,"All_mismatches":"NA","Worst_Case_MismatchLocal":"0","Primer_Sequence":"atggactggacctggaggttcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":60.38,"Self_Dimer_DeltaG":-2.11,"Structure_deltaG_fw":-1.86,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"634_openPrimeR2017"}
{"Primer":"Opt5_8","Template":"IGHV1-69-2","Group":"IGHV1-69-2","Number_of_mismatches":2,"primer_efficiency":0.22785,"annealing_DeltaG":-11.326,"Position_3terminus":4,"All_mismatches":"14,4","Worst_Case_MismatchLocal":"3,3","Primer_Sequence":"atggactggacctggaggttcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Amplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":60.38,"Self_Dimer_DeltaG":-2.11,"Structure_deltaG_fw":-1.86,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":3,"Number_of_mismatches_hexamer":1,"_row":"635_openPrimeR2017"}
{"Primer":"Opt5_8","Template":"IGHV1-8","Group":"IGHV1-8","Number_of_mismatches":1,"primer_efficiency":0.30248,"annealing_DeltaG":-13.283,"Position_3terminus":4,"All_mismatches":"4","Worst_Case_MismatchLocal":"3","Primer_Sequence":"atggactggacctggaggttcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Amplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":60.38,"Self_Dimer_DeltaG":-2.11,"Structure_deltaG_fw":-1.86,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":3,"Number_of_mismatches_hexamer":1,"_row":"636_openPrimeR2017"}
{"Primer":"Opt5_8","Template":"IGHV2-26","Group":"IGHV2-26","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-3.658,"Position_3terminus":3,"All_mismatches":"22,18,16,15,13,12,7,5,4,3","Worst_Case_MismatchLocal":"4,4,4,4,4,4,4,4,4,4","Primer_Sequence":"atggactggacctggaggttcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Amplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":60.38,"Self_Dimer_DeltaG":-2.11,"Structure_deltaG_fw":-1.86,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":4,"Number_of_mismatches_hexamer":3,"_row":"637_openPrimeR2017"}
{"Primer":"Opt5_8","Template":"IGHV2-5","Group":"IGHV2-5","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-1.695,"Position_3terminus":1,"All_mismatches":"21,19,17,16,14,11,7,5,4,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"atggactggacctggaggttcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":60.38,"Self_Dimer_DeltaG":-2.11,"Structure_deltaG_fw":-1.86,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":4,"_row":"638_openPrimeR2017"}
{"Primer":"Opt5_8","Template":"IGHV2-70","Group":"IGHV2-70","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-3.474,"Position_3terminus":3,"All_mismatches":"22,18,16,15,13,12,7,5,4,3","Worst_Case_MismatchLocal":"4,4,4,4,4,4,4,4,4,4","Primer_Sequence":"atggactggacctggaggttcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Amplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":60.38,"Self_Dimer_DeltaG":-2.11,"Structure_deltaG_fw":-1.86,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":4,"Number_of_mismatches_hexamer":3,"_row":"639_openPrimeR2017"}
{"Primer":"Opt5_8","Template":"IGHV3-11","Group":"IGHV3-11","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-2.72,"Position_3terminus":1,"All_mismatches":"22,21,15,14,13,11,5,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6","Primer_Sequence":"atggactggacctggaggttcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Amplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":60.38,"Self_Dimer_DeltaG":-2.11,"Structure_deltaG_fw":-1.86,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"640_openPrimeR2017"}
{"Primer":"Opt5_8","Template":"IGHV3-13","Group":"IGHV3-13","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-2.35,"Position_3terminus":5,"All_mismatches":"22,18,17,16,15,12,7,6,5","Worst_Case_MismatchLocal":"2,2,2,2,2,2,2,2,2","Primer_Sequence":"atggactggacctggaggttcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":60.38,"Self_Dimer_DeltaG":-2.11,"Structure_deltaG_fw":-1.86,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":2,"Number_of_mismatches_hexamer":2,"_row":"641_openPrimeR2017"}
{"Primer":"Opt5_8","Template":"IGHV3-15","Group":"IGHV3-15","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-2.72,"Position_3terminus":1,"All_mismatches":"22,21,15,14,13,11,5,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6","Primer_Sequence":"atggactggacctggaggttcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Amplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":60.38,"Self_Dimer_DeltaG":-2.11,"Structure_deltaG_fw":-1.86,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"642_openPrimeR2017"}
{"Primer":"Opt5_8","Template":"IGHV3-20","Group":"IGHV3-20","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-3.624,"Position_3terminus":3,"All_mismatches":"22,18,17,16,13,11,10,7,3","Worst_Case_MismatchLocal":"4,4,4,4,4,4,4,4,4","Primer_Sequence":"atggactggacctggaggttcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Amplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":60.38,"Self_Dimer_DeltaG":-2.11,"Structure_deltaG_fw":-1.86,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":4,"Number_of_mismatches_hexamer":1,"_row":"643_openPrimeR2017"}
{"Primer":"Opt5_8","Template":"IGHV3-21","Group":"IGHV3-21","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-1.599,"Position_3terminus":2,"All_mismatches":"22,21,19,14,13,11,10,9,4,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"atggactggacctggaggttcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":60.38,"Self_Dimer_DeltaG":-2.11,"Structure_deltaG_fw":-1.86,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":2,"_row":"644_openPrimeR2017"}
{"Primer":"Opt5_8","Template":"IGHV3-23","Group":"IGHV3-23","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-3.072,"Position_3terminus":2,"All_mismatches":"22,21,14,11,10,9,5,4,3,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"atggactggacctggaggttcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":60.38,"Self_Dimer_DeltaG":-2.11,"Structure_deltaG_fw":-1.86,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":4,"_row":"645_openPrimeR2017"}
{"Primer":"Opt5_8","Template":"IGHV3-30","Group":"IGHV3-30","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-2.72,"Position_3terminus":1,"All_mismatches":"22,21,15,14,13,11,5,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6","Primer_Sequence":"atggactggacctggaggttcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Amplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":60.38,"Self_Dimer_DeltaG":-2.11,"Structure_deltaG_fw":-1.86,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"646_openPrimeR2017"}
{"Primer":"Opt5_8","Template":"IGHV3-30-3","Group":"IGHV3-30-3","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-2.72,"Position_3terminus":1,"All_mismatches":"22,21,15,14,13,11,5,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6","Primer_Sequence":"atggactggacctggaggttcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Amplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":60.38,"Self_Dimer_DeltaG":-2.11,"Structure_deltaG_fw":-1.86,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"647_openPrimeR2017"}
{"Primer":"Opt5_8","Template":"IGHV3-33","Group":"IGHV3-33","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-2.72,"Position_3terminus":1,"All_mismatches":"22,21,15,14,13,11,5,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6","Primer_Sequence":"atggactggacctggaggttcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":60.38,"Self_Dimer_DeltaG":-2.11,"Structure_deltaG_fw":-1.86,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"648_openPrimeR2017"}
{"Primer":"Opt5_8","Template":"IGHV3-43","Group":"IGHV3-43","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-2.705,"Position_3terminus":2,"All_mismatches":"22,19,17,14,12,7,4,3,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5","Primer_Sequence":"atggactggacctggaggttcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":60.38,"Self_Dimer_DeltaG":-2.11,"Structure_deltaG_fw":-1.86,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":3,"_row":"649_openPrimeR2017"}
{"Primer":"Opt5_8","Template":"IGHV3-48","Group":"IGHV3-48","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-2.72,"Position_3terminus":2,"All_mismatches":"22,21,19,14,11,10,9,4,3,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"atggactggacctggaggttcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":60.38,"Self_Dimer_DeltaG":-2.11,"Structure_deltaG_fw":-1.86,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":3,"_row":"650_openPrimeR2017"}
{"Primer":"Opt5_8","Template":"IGHV3-49","Group":"IGHV3-49","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-4.144,"Position_3terminus":5,"All_mismatches":"22,21,20,17,16,15,14,13,10,5","Worst_Case_MismatchLocal":"2,2,2,2,2,2,2,2,2,2","Primer_Sequence":"atggactggacctggaggttcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":60.38,"Self_Dimer_DeltaG":-2.11,"Structure_deltaG_fw":-1.86,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":2,"Number_of_mismatches_hexamer":1,"_row":"651_openPrimeR2017"}
{"Primer":"Opt5_8","Template":"IGHV3-53","Group":"IGHV3-53","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-2.749,"Position_3terminus":1,"All_mismatches":"22,21,15,14,13,11,5,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6","Primer_Sequence":"atggactggacctggaggttcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":60.38,"Self_Dimer_DeltaG":-2.11,"Structure_deltaG_fw":-1.86,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"652_openPrimeR2017"}
{"Primer":"Opt5_8","Template":"IGHV3-64","Group":"IGHV3-64","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-2.35,"Position_3terminus":5,"All_mismatches":"22,20,18,17,16,15,12,7,6,5","Worst_Case_MismatchLocal":"2,2,2,2,2,2,2,2,2,2","Primer_Sequence":"atggactggacctggaggttcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":60.38,"Self_Dimer_DeltaG":-2.11,"Structure_deltaG_fw":-1.86,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":2,"Number_of_mismatches_hexamer":2,"_row":"653_openPrimeR2017"}
{"Primer":"Opt5_8","Template":"IGHV3-64D","Group":"IGHV3-64D","Number_of_mismatches":9,"primer_efficiency":0.00002,"annealing_DeltaG":-3.624,"Position_3terminus":3,"All_mismatches":"22,18,16,15,13,11,10,7,3","Worst_Case_MismatchLocal":"4,4,4,4,4,4,4,4,4","Primer_Sequence":"atggactggacctggaggttcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":60.38,"Self_Dimer_DeltaG":-2.11,"Structure_deltaG_fw":-1.86,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":4,"Number_of_mismatches_hexamer":1,"_row":"654_openPrimeR2017"}
{"Primer":"Opt5_8","Template":"IGHV3-66","Group":"IGHV3-66","Number_of_mismatches":8,"primer_efficiency":0,"annealing_DeltaG":-2.72,"Position_3terminus":1,"All_mismatches":"22,21,15,13,11,5,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6","Primer_Sequence":"atggactggacctggaggttcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":60.38,"Self_Dimer_DeltaG":-2.11,"Structure_deltaG_fw":-1.86,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"655_openPrimeR2017"}
{"Primer":"Opt5_8","Template":"IGHV3-7","Group":"IGHV3-7","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-2.35,"Position_3terminus":5,"All_mismatches":"22,18,17,16,15,12,7,6,5","Worst_Case_MismatchLocal":"2,2,2,2,2,2,2,2,2","Primer_Sequence":"atggactggacctggaggttcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":60.38,"Self_Dimer_DeltaG":-2.11,"Structure_deltaG_fw":-1.86,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":2,"Number_of_mismatches_hexamer":2,"_row":"656_openPrimeR2017"}
{"Primer":"Opt5_8","Template":"IGHV3-72","Group":"IGHV3-72","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-2.72,"Position_3terminus":1,"All_mismatches":"22,21,15,14,13,11,5,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6","Primer_Sequence":"atggactggacctggaggttcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Amplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":60.38,"Self_Dimer_DeltaG":-2.11,"Structure_deltaG_fw":-1.86,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"657_openPrimeR2017"}
{"Primer":"Opt5_8","Template":"IGHV3-73","Group":"IGHV3-73","Number_of_mismatches":9,"primer_efficiency":0.00002,"annealing_DeltaG":-3.624,"Position_3terminus":3,"All_mismatches":"22,18,16,15,13,11,10,7,3","Worst_Case_MismatchLocal":"4,4,4,4,4,4,4,4,4","Primer_Sequence":"atggactggacctggaggttcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":60.38,"Self_Dimer_DeltaG":-2.11,"Structure_deltaG_fw":-1.86,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":4,"Number_of_mismatches_hexamer":1,"_row":"658_openPrimeR2017"}
{"Primer":"Opt5_8","Template":"IGHV3-74","Group":"IGHV3-74","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-1.695,"Position_3terminus":3,"All_mismatches":"21,19,17,16,14,11,7,4,3","Worst_Case_MismatchLocal":"4,4,4,4,4,4,4,4,4","Primer_Sequence":"atggactggacctggaggttcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":60.38,"Self_Dimer_DeltaG":-2.11,"Structure_deltaG_fw":-1.86,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":4,"Number_of_mismatches_hexamer":2,"_row":"659_openPrimeR2017"}
{"Primer":"Opt5_8","Template":"IGHV3-9","Group":"IGHV3-9","Number_of_mismatches":8,"primer_efficiency":0,"annealing_DeltaG":-3.125,"Position_3terminus":5,"All_mismatches":"22,18,17,16,15,12,6,5","Worst_Case_MismatchLocal":"2,2,2,2,2,2,2,2","Primer_Sequence":"atggactggacctggaggttcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Amplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":60.38,"Self_Dimer_DeltaG":-2.11,"Structure_deltaG_fw":-1.86,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":2,"Number_of_mismatches_hexamer":2,"_row":"660_openPrimeR2017"}
{"Primer":"Opt5_8","Template":"IGHV4-28","Group":"IGHV4-28","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-3.026,"Position_3terminus":3,"All_mismatches":"22,21,18,12,10,7,6,4,3","Worst_Case_MismatchLocal":"4,4,4,4,4,4,4,4,4","Primer_Sequence":"atggactggacctggaggttcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":60.38,"Self_Dimer_DeltaG":-2.11,"Structure_deltaG_fw":-1.86,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":4,"Number_of_mismatches_hexamer":3,"_row":"661_openPrimeR2017"}
{"Primer":"Opt5_8","Template":"IGHV4-30-2","Group":"IGHV4-30-2","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-2.608,"Position_3terminus":1,"All_mismatches":"21,19,17,16,14,11,7,5,4,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"atggactggacctggaggttcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Amplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":60.38,"Self_Dimer_DeltaG":-2.11,"Structure_deltaG_fw":-1.86,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":4,"_row":"662_openPrimeR2017"}
{"Primer":"Opt5_8","Template":"IGHV4-31","Group":"IGHV4-31","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-1.695,"Position_3terminus":2,"All_mismatches":"21,19,17,16,14,11,7,4,3,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"atggactggacctggaggttcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Amplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":60.38,"Self_Dimer_DeltaG":-2.11,"Structure_deltaG_fw":-1.86,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":3,"_row":"663_openPrimeR2017"}
{"Primer":"Opt5_8","Template":"IGHV4-34","Group":"IGHV4-34","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-1.695,"Position_3terminus":2,"All_mismatches":"21,19,17,16,14,11,7,5,4,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"atggactggacctggaggttcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":60.38,"Self_Dimer_DeltaG":-2.11,"Structure_deltaG_fw":-1.86,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":3,"_row":"664_openPrimeR2017"}
{"Primer":"Opt5_8","Template":"IGHV4-38-2","Group":"IGHV4-38-2","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-3.324,"Position_3terminus":4,"All_mismatches":"22,19,14,13,12,7,6,5,4","Worst_Case_MismatchLocal":"3,3,3,3,3,3,3,3,3","Primer_Sequence":"atggactggacctggaggttcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":60.38,"Self_Dimer_DeltaG":-2.11,"Structure_deltaG_fw":-1.86,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":3,"Number_of_mismatches_hexamer":3,"_row":"665_openPrimeR2017"}
{"Primer":"Opt5_8","Template":"IGHV4-39","Group":"IGHV4-39","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-1.695,"Position_3terminus":1,"All_mismatches":"21,19,17,16,14,11,7,4,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"atggactggacctggaggttcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Amplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":60.38,"Self_Dimer_DeltaG":-2.11,"Structure_deltaG_fw":-1.86,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"666_openPrimeR2017"}
{"Primer":"Opt5_8","Template":"IGHV4-4","Group":"IGHV4-4","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-1.695,"Position_3terminus":1,"All_mismatches":"21,19,17,16,14,11,7,5,3,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"atggactggacctggaggttcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Amplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":60.38,"Self_Dimer_DeltaG":-2.11,"Structure_deltaG_fw":-1.86,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"667_openPrimeR2017"}
{"Primer":"Opt5_8","Template":"IGHV4-59","Group":"IGHV4-59","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-1.695,"Position_3terminus":2,"All_mismatches":"21,19,17,16,14,11,7,6,5,4,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"atggactggacctggaggttcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Amplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":60.38,"Self_Dimer_DeltaG":-2.11,"Structure_deltaG_fw":-1.86,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":4,"_row":"668_openPrimeR2017"}
{"Primer":"Opt5_8","Template":"IGHV4-61","Group":"IGHV4-61","Number_of_mismatches":11,"primer_efficiency":0,"annealing_DeltaG":-1.695,"Position_3terminus":1,"All_mismatches":"21,19,17,16,14,11,7,4,3,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"atggactggacctggaggttcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Amplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":60.38,"Self_Dimer_DeltaG":-2.11,"Structure_deltaG_fw":-1.86,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":4,"_row":"669_openPrimeR2017"}
{"Primer":"Opt5_8","Template":"IGHV5-10-1","Group":"IGHV5-10-1","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-3.517,"Position_3terminus":2,"All_mismatches":"21,20,19,12,10,8,7,4,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5","Primer_Sequence":"atggactggacctggaggttcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":60.38,"Self_Dimer_DeltaG":-2.11,"Structure_deltaG_fw":-1.86,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":2,"_row":"670_openPrimeR2017"}
{"Primer":"Opt5_8","Template":"IGHV5-51","Group":"IGHV5-51","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-3.084,"Position_3terminus":2,"All_mismatches":"21,19,17,16,14,11,7,5,4,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"atggactggacctggaggttcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":60.38,"Self_Dimer_DeltaG":-2.11,"Structure_deltaG_fw":-1.86,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":3,"_row":"671_openPrimeR2017"}
{"Primer":"Opt5_8","Template":"IGHV6-1","Group":"IGHV6-1","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-1.909,"Position_3terminus":2,"All_mismatches":"18,17,16,14,13,8,7,5,4,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"atggactggacctggaggttcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":60.38,"Self_Dimer_DeltaG":-2.11,"Structure_deltaG_fw":-1.86,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":3,"_row":"672_openPrimeR2017"}
{"Primer":"Opt5_8","Template":"IGHV7-4-1","Group":"IGHV7-4-1","Number_of_mismatches":1,"primer_efficiency":0.30248,"annealing_DeltaG":-13.283,"Position_3terminus":4,"All_mismatches":"4","Worst_Case_MismatchLocal":"3","Primer_Sequence":"atggactggacctggaggttcc","Terminal_Dinucleotide":"cc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Amplified","primer_length_fw":22,"gc_clamp_fw":2,"gc_ratio_fw":0.59091,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":60.38,"Self_Dimer_DeltaG":-2.11,"Structure_deltaG_fw":-1.86,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":3,"Number_of_mismatches_hexamer":1,"_row":"673_openPrimeR2017"}
{"Primer":"Opt5_9","Template":"IGHV1-18","Group":"IGHV1-18","Number_of_mismatches":2,"primer_efficiency":0.04233,"annealing_DeltaG":-9.184,"Position_3terminus":4,"All_mismatches":"13,4","Worst_Case_MismatchLocal":"3,3","Primer_Sequence":"atggactgcacctggaggatc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Amplified","primer_length_fw":21,"gc_clamp_fw":1,"gc_ratio_fw":0.57143,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":58.3,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":3,"Number_of_mismatches_hexamer":1,"_row":"674_openPrimeR2017"}
{"Primer":"Opt5_9","Template":"IGHV1-2","Group":"IGHV1-2","Number_of_mismatches":1,"primer_efficiency":0.75698,"annealing_DeltaG":-11.141,"Position_3terminus":13,"All_mismatches":"13","Worst_Case_MismatchLocal":"0","Primer_Sequence":"atggactgcacctggaggatc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":21,"gc_clamp_fw":1,"gc_ratio_fw":0.57143,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":58.3,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"675_openPrimeR2017"}
{"Primer":"Opt5_9","Template":"IGHV1-24","Group":"IGHV1-24","Number_of_mismatches":0,"primer_efficiency":0.99951,"annealing_DeltaG":-15.367,"All_mismatches":"NA","Worst_Case_MismatchLocal":"0","Primer_Sequence":"atggactgcacctggaggatc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":21,"gc_clamp_fw":1,"gc_ratio_fw":0.57143,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":58.3,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"676_openPrimeR2017"}
{"Primer":"Opt5_9","Template":"IGHV1-3","Group":"IGHV1-3","Number_of_mismatches":1,"primer_efficiency":0.75698,"annealing_DeltaG":-11.141,"Position_3terminus":13,"All_mismatches":"13","Worst_Case_MismatchLocal":"0","Primer_Sequence":"atggactgcacctggaggatc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":21,"gc_clamp_fw":1,"gc_ratio_fw":0.57143,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":58.3,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"677_openPrimeR2017"}
{"Primer":"Opt5_9","Template":"IGHV1-45","Group":"IGHV1-45","Number_of_mismatches":2,"primer_efficiency":0.01475,"annealing_DeltaG":-8.432,"Position_3terminus":4,"All_mismatches":"13,4","Worst_Case_MismatchLocal":"3,3","Primer_Sequence":"atggactgcacctggaggatc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Amplified","primer_length_fw":21,"gc_clamp_fw":1,"gc_ratio_fw":0.57143,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":58.3,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":3,"Number_of_mismatches_hexamer":1,"_row":"678_openPrimeR2017"}
{"Primer":"Opt5_9","Template":"IGHV1-46","Group":"IGHV1-46","Number_of_mismatches":2,"primer_efficiency":0.15396,"annealing_DeltaG":-10.168,"Position_3terminus":3,"All_mismatches":"13,3","Worst_Case_MismatchLocal":"4,4","Primer_Sequence":"atggactgcacctggaggatc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Amplified","primer_length_fw":21,"gc_clamp_fw":1,"gc_ratio_fw":0.57143,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":58.3,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":4,"Number_of_mismatches_hexamer":1,"_row":"679_openPrimeR2017"}
{"Primer":"Opt5_9","Template":"IGHV1-58","Group":"IGHV1-58","Number_of_mismatches":3,"primer_efficiency":0.00036,"annealing_DeltaG":-4.885,"Position_3terminus":10,"All_mismatches":"13,11,10","Worst_Case_MismatchLocal":"0,0,0","Primer_Sequence":"atggactgcacctggaggatc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":21,"gc_clamp_fw":1,"gc_ratio_fw":0.57143,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":58.3,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"680_openPrimeR2017"}
{"Primer":"Opt5_9","Template":"IGHV1-69","Group":"IGHV1-69","Number_of_mismatches":2,"primer_efficiency":0.25654,"annealing_DeltaG":-10.523,"Position_3terminus":3,"All_mismatches":"13,3","Worst_Case_MismatchLocal":"4,4","Primer_Sequence":"atggactgcacctggaggatc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Amplified","primer_length_fw":21,"gc_clamp_fw":1,"gc_ratio_fw":0.57143,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":58.3,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":4,"Number_of_mismatches_hexamer":1,"_row":"681_openPrimeR2017"}
{"Primer":"Opt5_9","Template":"IGHV1-69-2","Group":"IGHV1-69-2","Number_of_mismatches":0,"primer_efficiency":0.99951,"annealing_DeltaG":-15.367,"All_mismatches":"NA","Worst_Case_MismatchLocal":"0","Primer_Sequence":"atggactgcacctggaggatc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":21,"gc_clamp_fw":1,"gc_ratio_fw":0.57143,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":58.3,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"682_openPrimeR2017"}
{"Primer":"Opt5_9","Template":"IGHV1-8","Group":"IGHV1-8","Number_of_mismatches":1,"primer_efficiency":0.75698,"annealing_DeltaG":-11.141,"Position_3terminus":13,"All_mismatches":"13","Worst_Case_MismatchLocal":"0","Primer_Sequence":"atggactgcacctggaggatc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":21,"gc_clamp_fw":1,"gc_ratio_fw":0.57143,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":58.3,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"683_openPrimeR2017"}
{"Primer":"Opt5_9","Template":"IGHV2-26","Group":"IGHV2-26","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-1.695,"Position_3terminus":1,"All_mismatches":"20,18,16,15,13,10,6,4,3,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"atggactgcacctggaggatc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Amplified","primer_length_fw":21,"gc_clamp_fw":1,"gc_ratio_fw":0.57143,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":58.3,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":4,"_row":"684_openPrimeR2017"}
{"Primer":"Opt5_9","Template":"IGHV2-5","Group":"IGHV2-5","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-1.695,"Position_3terminus":1,"All_mismatches":"20,18,16,15,13,10,6,4,3,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"atggactgcacctggaggatc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":1,"gc_ratio_fw":0.57143,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":58.3,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":4,"_row":"685_openPrimeR2017"}
{"Primer":"Opt5_9","Template":"IGHV2-70","Group":"IGHV2-70","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-1.695,"Position_3terminus":1,"All_mismatches":"20,18,16,15,13,10,6,4,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"atggactgcacctggaggatc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":1,"gc_ratio_fw":0.57143,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":58.3,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":4,"_row":"686_openPrimeR2017"}
{"Primer":"Opt5_9","Template":"IGHV3-11","Group":"IGHV3-11","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-2.614,"Position_3terminus":1,"All_mismatches":"17,16,15,12,10,9,4,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6","Primer_Sequence":"atggactgcacctggaggatc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":1,"gc_ratio_fw":0.57143,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":58.3,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"687_openPrimeR2017"}
{"Primer":"Opt5_9","Template":"IGHV3-13","Group":"IGHV3-13","Number_of_mismatches":8,"primer_efficiency":0,"annealing_DeltaG":-2.908,"Position_3terminus":1,"All_mismatches":"21,18,17,16,11,6,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6","Primer_Sequence":"atggactgcacctggaggatc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":1,"gc_ratio_fw":0.57143,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":58.3,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"688_openPrimeR2017"}
{"Primer":"Opt5_9","Template":"IGHV3-15","Group":"IGHV3-15","Number_of_mismatches":8,"primer_efficiency":0,"annealing_DeltaG":-2.908,"Position_3terminus":1,"All_mismatches":"21,18,17,16,11,6,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6","Primer_Sequence":"atggactgcacctggaggatc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":1,"gc_ratio_fw":0.57143,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":58.3,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"689_openPrimeR2017"}
{"Primer":"Opt5_9","Template":"IGHV3-20","Group":"IGHV3-20","Number_of_mismatches":8,"primer_efficiency":0,"annealing_DeltaG":-2.908,"Position_3terminus":1,"All_mismatches":"21,18,17,16,11,6,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6","Primer_Sequence":"atggactgcacctggaggatc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":1,"gc_ratio_fw":0.57143,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":58.3,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"690_openPrimeR2017"}
{"Primer":"Opt5_9","Template":"IGHV3-21","Group":"IGHV3-21","Number_of_mismatches":8,"primer_efficiency":0,"annealing_DeltaG":-2.908,"Position_3terminus":1,"All_mismatches":"21,18,17,16,11,6,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6","Primer_Sequence":"atggactgcacctggaggatc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":1,"gc_ratio_fw":0.57143,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":58.3,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"691_openPrimeR2017"}
{"Primer":"Opt5_9","Template":"IGHV3-23","Group":"IGHV3-23","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-3.063,"Position_3terminus":1,"All_mismatches":"21,20,13,10,9,8,4,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6","Primer_Sequence":"atggactgcacctggaggatc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":1,"gc_ratio_fw":0.57143,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":58.3,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"692_openPrimeR2017"}
{"Primer":"Opt5_9","Template":"IGHV3-30","Group":"IGHV3-30","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-2.232,"Position_3terminus":1,"All_mismatches":"21,20,18,13,10,9,8,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6","Primer_Sequence":"atggactgcacctggaggatc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":1,"gc_ratio_fw":0.57143,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":58.3,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":2,"_row":"693_openPrimeR2017"}
{"Primer":"Opt5_9","Template":"IGHV3-30-3","Group":"IGHV3-30-3","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-1.695,"Position_3terminus":1,"All_mismatches":"20,18,16,15,13,10,6,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6","Primer_Sequence":"atggactgcacctggaggatc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":1,"gc_ratio_fw":0.57143,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":58.3,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"694_openPrimeR2017"}
{"Primer":"Opt5_9","Template":"IGHV3-33","Group":"IGHV3-33","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-2.232,"Position_3terminus":1,"All_mismatches":"21,20,18,13,10,9,8,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6","Primer_Sequence":"atggactgcacctggaggatc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":1,"gc_ratio_fw":0.57143,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":58.3,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":2,"_row":"695_openPrimeR2017"}
{"Primer":"Opt5_9","Template":"IGHV3-43","Group":"IGHV3-43","Number_of_mismatches":7,"primer_efficiency":0,"annealing_DeltaG":-2.908,"Position_3terminus":1,"All_mismatches":"21,18,16,11,6,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6","Primer_Sequence":"atggactgcacctggaggatc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":1,"gc_ratio_fw":0.57143,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":58.3,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"696_openPrimeR2017"}
{"Primer":"Opt5_9","Template":"IGHV3-48","Group":"IGHV3-48","Number_of_mismatches":8,"primer_efficiency":0,"annealing_DeltaG":-2.908,"Position_3terminus":1,"All_mismatches":"21,18,17,16,11,6,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6","Primer_Sequence":"atggactgcacctggaggatc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":1,"gc_ratio_fw":0.57143,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":58.3,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"697_openPrimeR2017"}
{"Primer":"Opt5_9","Template":"IGHV3-49","Group":"IGHV3-49","Number_of_mismatches":8,"primer_efficiency":0,"annealing_DeltaG":-2.908,"Position_3terminus":1,"All_mismatches":"21,18,17,16,11,6,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6","Primer_Sequence":"atggactgcacctggaggatc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":1,"gc_ratio_fw":0.57143,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":58.3,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"698_openPrimeR2017"}
{"Primer":"Opt5_9","Template":"IGHV3-53","Group":"IGHV3-53","Number_of_mismatches":8,"primer_efficiency":0,"annealing_DeltaG":-2.908,"Position_3terminus":1,"All_mismatches":"21,18,17,16,11,6,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6","Primer_Sequence":"atggactgcacctggaggatc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":1,"gc_ratio_fw":0.57143,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":58.3,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"699_openPrimeR2017"}
{"Primer":"Opt5_9","Template":"IGHV3-64","Group":"IGHV3-64","Number_of_mismatches":8,"primer_efficiency":0,"annealing_DeltaG":-2.908,"Position_3terminus":1,"All_mismatches":"21,18,17,16,11,6,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6","Primer_Sequence":"atggactgcacctggaggatc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":1,"gc_ratio_fw":0.57143,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":58.3,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"700_openPrimeR2017"}
{"Primer":"Opt5_9","Template":"IGHV3-64D","Group":"IGHV3-64D","Number_of_mismatches":8,"primer_efficiency":0,"annealing_DeltaG":-2.908,"Position_3terminus":1,"All_mismatches":"21,18,17,16,11,6,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6","Primer_Sequence":"atggactgcacctggaggatc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":1,"gc_ratio_fw":0.57143,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":58.3,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"701_openPrimeR2017"}
{"Primer":"Opt5_9","Template":"IGHV3-66","Group":"IGHV3-66","Number_of_mismatches":8,"primer_efficiency":0,"annealing_DeltaG":-3.632,"Position_3terminus":1,"All_mismatches":"21,18,13,10,9,8,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6","Primer_Sequence":"atggactgcacctggaggatc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":1,"gc_ratio_fw":0.57143,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":58.3,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":2,"_row":"702_openPrimeR2017"}
{"Primer":"Opt5_9","Template":"IGHV3-7","Group":"IGHV3-7","Number_of_mismatches":8,"primer_efficiency":0,"annealing_DeltaG":-2.908,"Position_3terminus":1,"All_mismatches":"21,18,17,16,11,6,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6","Primer_Sequence":"atggactgcacctggaggatc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":1,"gc_ratio_fw":0.57143,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":58.3,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"703_openPrimeR2017"}
{"Primer":"Opt5_9","Template":"IGHV3-72","Group":"IGHV3-72","Number_of_mismatches":8,"primer_efficiency":0,"annealing_DeltaG":-2.908,"Position_3terminus":1,"All_mismatches":"21,18,17,16,11,6,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6","Primer_Sequence":"atggactgcacctggaggatc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":1,"gc_ratio_fw":0.57143,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":58.3,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"704_openPrimeR2017"}
{"Primer":"Opt5_9","Template":"IGHV3-73","Group":"IGHV3-73","Number_of_mismatches":8,"primer_efficiency":0,"annealing_DeltaG":-2.908,"Position_3terminus":1,"All_mismatches":"21,18,17,16,11,6,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6","Primer_Sequence":"atggactgcacctggaggatc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":1,"gc_ratio_fw":0.57143,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":58.3,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"705_openPrimeR2017"}
{"Primer":"Opt5_9","Template":"IGHV3-74","Group":"IGHV3-74","Number_of_mismatches":8,"primer_efficiency":0,"annealing_DeltaG":-1.695,"Position_3terminus":2,"All_mismatches":"20,18,16,15,13,10,6,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5","Primer_Sequence":"atggactgcacctggaggatc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":1,"gc_ratio_fw":0.57143,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":58.3,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":2,"_row":"706_openPrimeR2017"}
{"Primer":"Opt5_9","Template":"IGHV3-9","Group":"IGHV3-9","Number_of_mismatches":7,"primer_efficiency":0,"annealing_DeltaG":-2.908,"Position_3terminus":1,"All_mismatches":"21,18,16,11,6,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6","Primer_Sequence":"atggactgcacctggaggatc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":1,"gc_ratio_fw":0.57143,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":58.3,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"707_openPrimeR2017"}
{"Primer":"Opt5_9","Template":"IGHV4-28","Group":"IGHV4-28","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-2.782,"Position_3terminus":1,"All_mismatches":"20,18,15,12,11,9,4,3,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"atggactgcacctggaggatc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":1,"gc_ratio_fw":0.57143,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":58.3,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":4,"_row":"708_openPrimeR2017"}
{"Primer":"Opt5_9","Template":"IGHV4-30-2","Group":"IGHV4-30-2","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-4.633,"Position_3terminus":1,"All_mismatches":"21,20,19,18,17,11,7,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6","Primer_Sequence":"atggactgcacctggaggatc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":1,"gc_ratio_fw":0.57143,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":58.3,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":2,"_row":"709_openPrimeR2017"}
{"Primer":"Opt5_9","Template":"IGHV4-31","Group":"IGHV4-31","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-2.712,"Position_3terminus":2,"All_mismatches":"21,15,14,12,11,10,7,5,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5","Primer_Sequence":"atggactgcacctggaggatc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":1,"gc_ratio_fw":0.57143,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":58.3,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":2,"_row":"710_openPrimeR2017"}
{"Primer":"Opt5_9","Template":"IGHV4-34","Group":"IGHV4-34","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-1.695,"Position_3terminus":1,"All_mismatches":"20,18,16,15,13,10,6,4,3,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6,6","Primer_Sequence":"atggactgcacctggaggatc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":1,"gc_ratio_fw":0.57143,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":58.3,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":4,"_row":"711_openPrimeR2017"}
{"Primer":"Opt5_9","Template":"IGHV4-38-2","Group":"IGHV4-38-2","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-3.011,"Position_3terminus":2,"All_mismatches":"21,20,14,13,12,10,7,4,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5","Primer_Sequence":"atggactgcacctggaggatc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":2,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":1,"gc_ratio_fw":0.57143,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":58.3,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":2,"_row":"712_openPrimeR2017"}
{"Primer":"Opt5_9","Template":"IGHV4-39","Group":"IGHV4-39","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-1.695,"Position_3terminus":1,"All_mismatches":"20,18,16,15,13,10,6,3,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6","Primer_Sequence":"atggactgcacctggaggatc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":1,"gc_ratio_fw":0.57143,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":58.3,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"713_openPrimeR2017"}
{"Primer":"Opt5_9","Template":"IGHV4-4","Group":"IGHV4-4","Number_of_mismatches":10,"primer_efficiency":0,"annealing_DeltaG":-1.695,"Position_3terminus":2,"All_mismatches":"20,18,16,15,13,10,6,4,3,2","Worst_Case_MismatchLocal":"5,5,5,5,5,5,5,5,5,5","Primer_Sequence":"atggactgcacctggaggatc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":1,"Mismatch_pos_5":1,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":1,"gc_ratio_fw":0.57143,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":58.3,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":5,"Number_of_mismatches_hexamer":4,"_row":"714_openPrimeR2017"}
{"Primer":"Opt5_9","Template":"IGHV4-59","Group":"IGHV4-59","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-2.677,"Position_3terminus":1,"All_mismatches":"20,18,16,15,10,6,5,4,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6","Primer_Sequence":"atggactgcacctggaggatc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":4,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":1,"gc_ratio_fw":0.57143,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":58.3,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":4,"_row":"715_openPrimeR2017"}
{"Primer":"Opt5_9","Template":"IGHV4-61","Group":"IGHV4-61","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-1.695,"Position_3terminus":1,"All_mismatches":"20,18,16,15,13,10,6,2,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6","Primer_Sequence":"atggactgcacctggaggatc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":1,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":1,"gc_ratio_fw":0.57143,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":58.3,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"716_openPrimeR2017"}
{"Primer":"Opt5_9","Template":"IGHV5-10-1","Group":"IGHV5-10-1","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-4.414,"Position_3terminus":3,"All_mismatches":"21,20,18,17,14,12,6,5,3","Worst_Case_MismatchLocal":"4,4,4,4,4,4,4,4,4","Primer_Sequence":"atggactgcacctggaggatc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":1,"Mismatch_pos_2":1,"Mismatch_pos_3":0,"Mismatch_pos_4":1,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":1,"gc_ratio_fw":0.57143,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":58.3,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":4,"Number_of_mismatches_hexamer":3,"_row":"717_openPrimeR2017"}
{"Primer":"Opt5_9","Template":"IGHV5-51","Group":"IGHV5-51","Number_of_mismatches":8,"primer_efficiency":0,"annealing_DeltaG":-4.863,"Position_3terminus":6,"All_mismatches":"21,20,12,10,9,8,7,6","Worst_Case_MismatchLocal":"1,1,1,1,1,1,1,1","Primer_Sequence":"atggactgcacctggaggatc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":1,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":1,"gc_ratio_fw":0.57143,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":58.3,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":1,"Number_of_mismatches_hexamer":1,"_row":"718_openPrimeR2017"}
{"Primer":"Opt5_9","Template":"IGHV6-1","Group":"IGHV6-1","Number_of_mismatches":9,"primer_efficiency":0,"annealing_DeltaG":-2.582,"Position_3terminus":1,"All_mismatches":"17,16,15,13,12,7,6,4,1","Worst_Case_MismatchLocal":"6,6,6,6,6,6,6,6,6","Primer_Sequence":"atggactgcacctggaggatc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":1,"Mismatch_pos_2":0,"Mismatch_pos_3":1,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":1,"Hexamer_Mismatch_Count":3,"Experimental_Coverage":"Unamplified","primer_length_fw":21,"gc_clamp_fw":1,"gc_ratio_fw":0.57143,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":58.3,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":6,"Number_of_mismatches_hexamer":3,"_row":"719_openPrimeR2017"}
{"Primer":"Opt5_9","Template":"IGHV7-4-1","Group":"IGHV7-4-1","Number_of_mismatches":1,"primer_efficiency":0.75698,"annealing_DeltaG":-11.141,"Position_3terminus":13,"All_mismatches":"13","Worst_Case_MismatchLocal":"0","Primer_Sequence":"atggactgcacctggaggatc","Terminal_Dinucleotide":"tc","Mismatch_pos_1":0,"Mismatch_pos_2":0,"Mismatch_pos_3":0,"Mismatch_pos_4":0,"Mismatch_pos_5":0,"Mismatch_pos_6":0,"Hexamer_Mismatch_Count":0,"Experimental_Coverage":"Amplified","primer_length_fw":21,"gc_clamp_fw":1,"gc_ratio_fw":0.57143,"no_runs_fw":2,"no_repeats_fw":1,"melting_temp":58.3,"Self_Dimer_DeltaG":-1.877,"Structure_deltaG_fw":0,"primer_coverage":47,"primer_specificity":0.5,"Run":"openPrimeR2017","Position_3terminusLocal":0,"Number_of_mismatches_hexamer":0,"_row":"720_openPrimeR2017"}