Soneson C, Bendel A, Diss G, Stadler M (2023). “mutscan-a flexible R package for efficient end-to-end analysis of multiplexed assays of variant effect data.” Genome Biology, 24, 132. ISSN 1474-760X. doi:10.1186/s13059-023-02967-0. https://doi.org/10.1186/s13059-023-02967-0.
Corresponding BibTeX entry:
@Article{,
title = {mutscan-a flexible R package for efficient end-to-end
analysis of multiplexed assays of variant effect data},
author = {Charlotte Soneson and Alexandra M. Bendel and Guillaume
Diss and Michael B. Stadler},
publisher = {Springer Nature},
journal = {Genome Biology},
year = {2023},
month = {Jun},
volume = {24},
pages = {132},
doi = {10.1186/s13059-023-02967-0},
issn = {1474-760X},
url = {https://doi.org/10.1186/s13059-023-02967-0},
abstract = {Multiplexed assays of variant effect (MAVE)
experimentally measure the effect of large numbers of sequence
variants by selective enrichment of sequences with desirable
properties followed by quantification by sequencing. mutscan is
an R package for flexible analysis of such experiments, covering
the entire workflow from raw reads up to statistical analysis and
visualization. The core components are implemented in C++ for
efficiency. Various experimental designs are supported, including
single or paired reads with optional unique molecular
identifiers. To find variants with changed relative abundance,
mutscan employs established statistical models provided in the
edgeR and limma packages. mutscan is available from
https://github.com/fmicompbio/mutscan.},
}