Decontamination of single cell protein expression data with DecontPro
Introduction
DecontPro assess and decontaminate single-cell protein expression data, such as those generated from CITE-seq or Total-seq. The count matrix is decomposed into three matrices, the native, the ambient and the background that represent the contribution from the true protein expression on cells, the ambient material and other non-specific background contamination.
Installation
DecontX Package can be installed from Bioconductor:
if (!requireNamespace("BiocManager", quietly = TRUE)) {
install.packages("BiocManager")
}
BiocManager::install("decontX")Then the package can be loaded in R using the following command:
To see the latest updates and releases or to post a bug, see our GitHub page at https://github.com/campbio/decontX.
Importing data
Here we use an example dataset from SingleCellMultiModal
package.
library(SingleCellMultiModal)
dat <- CITEseq("cord_blood", dry.run = FALSE)
#> Warning: 'ExperimentList' contains 'data.frame' or 'DataFrame',
#> potential for errors with mixed data types
counts <- experiments(dat)$scADTFor this tutorial, we sample only 1000 droplets from the dataset to
demonstrate the use of functions. When analyzing your dataset,
sub-sampling should be done with caution, as decontPro
approximates contamination profile using the dataset. A biased sampling
may introduce bias to the contamination profile approximation.
Generate cell clusters
decontPro requires a vector indicating the cell types of
each droplet. Here we use Seurat for clustering.
library(Seurat)
library(dplyr)
adt_seurat <- CreateSeuratObject(counts_sample, assay = "ADT")
#> Warning: Data is of class matrix. Coercing to dgCMatrix.
adt_seurat <- NormalizeData(adt_seurat, normalization.method = "CLR", margin = 2) %>%
ScaleData(assay = "ADT") %>%
RunPCA(assay = "ADT", features = rownames(adt_seurat), npcs = 10,
reduction.name = "pca_adt") %>%
FindNeighbors(dims = 1:10, assay = "ADT", reduction = "pca_adt") %>%
FindClusters(resolution = 0.5)
#> Warning in irlba(A = t(x = object), nv = npcs, ...): You're computing too large
#> a percentage of total singular values, use a standard svd instead.
#> Warning: Requested number is larger than the number of available items (13).
#> Setting to 13.
#> Warning: Requested number is larger than the number of available items (13).
#> Setting to 13.
#> Warning: Requested number is larger than the number of available items (13).
#> Setting to 13.
#> Warning: Requested number is larger than the number of available items (13).
#> Setting to 13.
#> Warning: Requested number is larger than the number of available items (13).
#> Setting to 13.
#> Modularity Optimizer version 1.3.0 by Ludo Waltman and Nees Jan van Eck
#>
#> Number of nodes: 1000
#> Number of edges: 32498
#>
#> Running Louvain algorithm...
#> Maximum modularity in 10 random starts: 0.8567
#> Number of communities: 9
#> Elapsed time: 0 secondsadt_seurat <- RunUMAP(adt_seurat,
dims = 1:10,
assay = "ADT",
reduction = "pca_adt",
reduction.name = "adtUMAP",
verbose = FALSE)
#> Warning: The default method for RunUMAP has changed from calling Python UMAP via reticulate to the R-native UWOT using the cosine metric
#> To use Python UMAP via reticulate, set umap.method to 'umap-learn' and metric to 'correlation'
#> This message will be shown once per session
DimPlot(adt_seurat, reduction = "adtUMAP", label = TRUE)
Run DecontPro
You can run DecontPro by simply:
Priors (delta_sd and background_sd) may
need tuning with the help of plotting the decontaminated results. The
two priors encode belief if a more spread-out count should be considered
contamination vs. native. We tested the default values on datasets
ranging 5k to 10k droplets and 10 to 30 ADTs and the results are
reasonable. A more contaminated or a smaller dataset may need larger
priors. In this tutorial, since we sampled only 1k droplets from the
original 10k droplets, we use slightly larger priors:
counts <- as.matrix(counts_sample)
out <- decontPro(counts,
clusters,
delta_sd = 2e-4,
background_sd = 2e-5)
#> Chain 1: ------------------------------------------------------------
#> Chain 1: EXPERIMENTAL ALGORITHM:
#> Chain 1: This procedure has not been thoroughly tested and may be unstable
#> Chain 1: or buggy. The interface is subject to change.
#> Chain 1: ------------------------------------------------------------
#> Chain 1:
#> Chain 1:
#> Chain 1:
#> Chain 1: Gradient evaluation took 0.008034 seconds
#> Chain 1: 1000 transitions using 10 leapfrog steps per transition would take 80.34 seconds.
#> Chain 1: Adjust your expectations accordingly!
#> Chain 1:
#> Chain 1:
#> Chain 1: Begin eta adaptation.
#> Chain 1: Iteration: 1 / 250 [ 0%] (Adaptation)
#> Chain 1: Iteration: 50 / 250 [ 20%] (Adaptation)
#> Chain 1: Iteration: 100 / 250 [ 40%] (Adaptation)
#> Chain 1: Iteration: 150 / 250 [ 60%] (Adaptation)
#> Chain 1: Iteration: 200 / 250 [ 80%] (Adaptation)
#> Chain 1: Success! Found best value [eta = 1] earlier than expected.
#> Chain 1:
#> Chain 1: Begin stochastic gradient ascent.
#> Chain 1: iter ELBO delta_ELBO_mean delta_ELBO_med notes
#> Chain 1: 100 -513803659.665 1.000 1.000
#> Chain 1: 200 -7177548.141 35.792 70.585
#> Chain 1: 300 -342758.348 30.508 19.941
#> Chain 1: 400 -253644.497 22.969 19.941
#> Chain 1: 500 -223785.346 18.402 1.000
#> Chain 1: 600 -212411.740 15.344 1.000
#> Chain 1: 700 -206476.954 13.156 0.351
#> Chain 1: 800 -202104.000 11.514 0.351
#> Chain 1: 900 -199781.145 10.236 0.133
#> Chain 1: 1000 -198758.154 9.213 0.133
#> Chain 1: 1100 -198020.264 8.376 0.054 MAY BE DIVERGING... INSPECT ELBO
#> Chain 1: 1200 -197397.014 7.678 0.054 MAY BE DIVERGING... INSPECT ELBO
#> Chain 1: 1300 -196906.436 7.088 0.029 MAY BE DIVERGING... INSPECT ELBO
#> Chain 1: 1400 -196442.570 6.582 0.029 MAY BE DIVERGING... INSPECT ELBO
#> Chain 1: 1500 -196230.683 6.143 0.022 MAY BE DIVERGING... INSPECT ELBO
#> Chain 1: 1600 -195981.145 5.759 0.022 MAY BE DIVERGING... INSPECT ELBO
#> Chain 1: 1700 -195830.350 5.420 0.012 MAY BE DIVERGING... INSPECT ELBO
#> Chain 1: 1800 -195732.312 5.119 0.012 MAY BE DIVERGING... INSPECT ELBO
#> Chain 1: 1900 -195651.552 4.850 0.005 MEDIAN ELBO CONVERGED MAY BE DIVERGING... INSPECT ELBO
#> Chain 1:
#> Chain 1: Drawing a sample of size 1000 from the approximate posterior...
#> Chain 1: COMPLETED.
#> Warning: Pareto k diagnostic value is 41.49. Resampling is disabled. Decreasing
#> tol_rel_obj may help if variational algorithm has terminated prematurely.
#> Otherwise consider using sampling instead.The output contains three matrices, and model parameters after
inference. decontaminated_counts represent the true protein
expression on cells.
Plot Results
Plot ADT density before and after decontamination. For bimodal ADTs, the background peak should be removed. Note CD4 is tri-modal with the intermediate mode corresponding to monocytes. This can be used as a QC metric for decontamination as only the lowest mode should be removed.
We can also visualize the decontamination by each cell cluster.
Session Information
sessionInfo()
#> R version 4.4.1 (2024-06-14)
#> Platform: x86_64-pc-linux-gnu
#> Running under: Ubuntu 24.04.1 LTS
#>
#> Matrix products: default
#> BLAS: /usr/lib/x86_64-linux-gnu/openblas-pthread/libblas.so.3
#> LAPACK: /usr/lib/x86_64-linux-gnu/openblas-pthread/libopenblasp-r0.3.26.so; LAPACK version 3.12.0
#>
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#>
#> time zone: Etc/UTC
#> tzcode source: system (glibc)
#>
#> attached base packages:
#> [1] stats4 stats graphics grDevices utils datasets methods
#> [8] base
#>
#> other attached packages:
#> [1] dplyr_1.1.4 Seurat_5.1.0
#> [3] SeuratObject_5.0.2 sp_2.1-4
#> [5] SingleCellMultiModal_1.17.4 MultiAssayExperiment_1.31.5
#> [7] SummarizedExperiment_1.35.5 Biobase_2.67.0
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#> [13] BiocGenerics_0.53.0 MatrixGenerics_1.17.1
#> [15] matrixStats_1.4.1 decontX_1.5.0
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