Package: ddCt Title: The ddCt Algorithm for the Analysis of Quantitative Real-Time PCR (qRT-PCR) Version: 1.69.0 Date: 2021-07-30 Author: Jitao David Zhang, Rudolf Biczok, and Markus Ruschhaupt Description: The Delta-Delta-Ct (ddCt) Algorithm is an approximation method to determine relative gene expression with quantitative real-time PCR (qRT-PCR) experiments. Compared to other approaches, it requires no standard curve for each primer-target pair, therefore reducing the working load and yet returning accurate enough results as long as the assumptions of the amplification efficiency hold. The ddCt package implements a pipeline to collect, analyse and visualize qRT-PCR results, for example those from TaqMan SDM software, mainly using the ddCt method. The pipeline can be either invoked by a script in command-line or through the API consisting of S4-Classes, methods and functions. Maintainer: Jitao David Zhang biocViews: GeneExpression, DifferentialExpression, MicrotitrePlateAssay, qPCR License: LGPL-3 Depends: R (>= 2.3.0), methods Imports: Biobase (>= 1.10.0), RColorBrewer (>= 0.1-3), xtable, lattice, BiocGenerics Collate: AllGlobals.R AllClasses.R AllGenerics.R AllFunctions.R AllMethods.R absoluteQ.R ddCtSplit.R output.R visualization.R Suggests: testthat (>= 3.0.0), RUnit Config/testthat/edition: 3 Repository: https://bioc.r-universe.dev Date/Publication: 2026-04-28 12:34:05 UTC RemoteUrl: https://github.com/bioc/ddCt RemoteRef: HEAD RemoteSha: 7b2178781b6f50f19e3109914dcaaa48cb3abb90 NeedsCompilation: no Packaged: 2026-07-05 01:51:12 UTC; root