crisprVerse: ecosystem of R packages for CRISPR gRNA design

Installation and getting started

The crisprVerse is a collection of packages for CRISPR guide RNA (gRNA) design that can easily be installed with the crisprVerse package. This provides a convenient way of downloading and installing all crisprVerse packages with a single R command.

The package can be installed from the Bioconductor devel branch using the following commands in an R session:

if (!requireNamespace("BiocManager", quietly = TRUE))
    install.packages("BiocManager")

BiocManager::install(version="devel")
BiocManager::install("crisprVerse")

The core crisprVerse includes the packages that are commonly used for gRNA design, and are attached when you attach the crisprVerse package:

library(crisprVerse)

You can check that all crisprVerse packages are up-to-date with the function crisprVerse_update().

Components

The following packages are installed and loaded with the crisprVerse package:

Reproducibility

sessionInfo()
## R version 4.4.1 (2024-06-14)
## Platform: x86_64-pc-linux-gnu
## Running under: Ubuntu 24.04 LTS
## 
## Matrix products: default
## BLAS:   /usr/lib/x86_64-linux-gnu/openblas-pthread/libblas.so.3 
## LAPACK: /usr/lib/x86_64-linux-gnu/openblas-pthread/libopenblasp-r0.3.26.so;  LAPACK version 3.12.0
## 
## locale:
##  [1] LC_CTYPE=en_US.UTF-8       LC_NUMERIC=C              
##  [3] LC_TIME=en_US.UTF-8        LC_COLLATE=C              
##  [5] LC_MONETARY=en_US.UTF-8    LC_MESSAGES=en_US.UTF-8   
##  [7] LC_PAPER=en_US.UTF-8       LC_NAME=C                 
##  [9] LC_ADDRESS=C               LC_TELEPHONE=C            
## [11] LC_MEASUREMENT=en_US.UTF-8 LC_IDENTIFICATION=C       
## 
## time zone: Etc/UTC
## tzcode source: system (glibc)
## 
## attached base packages:
## [1] stats     graphics  grDevices utils     datasets  methods   base     
## 
## other attached packages:
##  [1] crisprViz_1.7.0       crisprDesign_1.7.2    crisprScore_1.9.2    
##  [4] crisprScoreData_1.9.0 ExperimentHub_2.13.1  AnnotationHub_3.13.1 
##  [7] BiocFileCache_2.13.0  dbplyr_2.5.0          BiocGenerics_0.51.0  
## [10] crisprBowtie_1.9.0    crisprBase_1.9.1      crisprVerse_1.7.0    
## [13] BiocStyle_2.33.1     
## 
## loaded via a namespace (and not attached):
##   [1] RColorBrewer_1.1-3          rstudioapi_0.16.0          
##   [3] sys_3.4.2                   jsonlite_1.8.8             
##   [5] magrittr_2.0.3              GenomicFeatures_1.57.0     
##   [7] rmarkdown_2.28              BiocIO_1.15.1              
##   [9] zlibbioc_1.51.1             vctrs_0.6.5                
##  [11] memoise_2.0.1               Rsamtools_2.21.1           
##  [13] RCurl_1.98-1.16             base64enc_0.1-3            
##  [15] htmltools_0.5.8.1           S4Arrays_1.5.7             
##  [17] progress_1.2.3              curl_5.2.1                 
##  [19] SparseArray_1.5.31          Formula_1.2-5              
##  [21] sass_0.4.9                  bslib_0.8.0                
##  [23] htmlwidgets_1.6.4           basilisk_1.17.2            
##  [25] Gviz_1.49.0                 httr2_1.0.2                
##  [27] cachem_1.1.0                buildtools_1.0.0           
##  [29] GenomicAlignments_1.41.0    lifecycle_1.0.4            
##  [31] pkgconfig_2.0.3             Matrix_1.7-0               
##  [33] R6_2.5.1                    fastmap_1.2.0              
##  [35] GenomeInfoDbData_1.2.12     MatrixGenerics_1.17.0      
##  [37] digest_0.6.36               colorspace_2.1-1           
##  [39] AnnotationDbi_1.67.0        S4Vectors_0.43.2           
##  [41] Hmisc_5.1-3                 GenomicRanges_1.57.1       
##  [43] RSQLite_2.3.7               filelock_1.0.3             
##  [45] randomForest_4.7-1.1        fansi_1.0.6                
##  [47] httr_1.4.7                  abind_1.4-5                
##  [49] compiler_4.4.1              Rbowtie_1.45.0             
##  [51] bit64_4.0.5                 backports_1.5.0            
##  [53] htmlTable_2.4.3             BiocParallel_1.39.0        
##  [55] DBI_1.2.3                   biomaRt_2.61.3             
##  [57] rappdirs_0.3.3              DelayedArray_0.31.11       
##  [59] rjson_0.2.21                tools_4.4.1                
##  [61] foreign_0.8-87              nnet_7.3-19                
##  [63] glue_1.7.0                  restfulr_0.0.15            
##  [65] grid_4.4.1                  checkmate_2.3.2            
##  [67] cluster_2.1.6               generics_0.1.3             
##  [69] gtable_0.3.5                BSgenome_1.73.0            
##  [71] tzdb_0.4.0                  ensembldb_2.29.0           
##  [73] data.table_1.15.4           hms_1.1.3                  
##  [75] xml2_1.3.6                  utf8_1.2.4                 
##  [77] XVector_0.45.0              BiocVersion_3.20.0         
##  [79] pillar_1.9.0                stringr_1.5.1              
##  [81] dplyr_1.1.4                 lattice_0.22-6             
##  [83] deldir_2.0-4                rtracklayer_1.65.0         
##  [85] bit_4.0.5                   biovizBase_1.53.0          
##  [87] tidyselect_1.2.1            maketools_1.3.0            
##  [89] Biostrings_2.73.1           knitr_1.48                 
##  [91] gridExtra_2.3               ProtGenerics_1.37.1        
##  [93] IRanges_2.39.2              SummarizedExperiment_1.35.1
##  [95] stats4_4.4.1                xfun_0.47                  
##  [97] Biobase_2.65.0              matrixStats_1.3.0          
##  [99] stringi_1.8.4               UCSC.utils_1.1.0           
## [101] lazyeval_0.2.2              yaml_2.3.10                
## [103] evaluate_0.24.0             codetools_0.2-20           
## [105] interp_1.1-6                tibble_3.2.1               
## [107] BiocManager_1.30.23         cli_3.6.3                  
## [109] rpart_4.1.23                reticulate_1.38.0          
## [111] munsell_0.5.1               jquerylib_0.1.4            
## [113] dichromat_2.0-0.1           Rcpp_1.0.13                
## [115] GenomeInfoDb_1.41.1         dir.expiry_1.13.0          
## [117] png_0.1-8                   XML_3.99-0.17              
## [119] parallel_4.4.1              ggplot2_3.5.1              
## [121] readr_2.1.5                 blob_1.2.4                 
## [123] basilisk.utils_1.17.2       prettyunits_1.2.0          
## [125] jpeg_0.1-10                 latticeExtra_0.6-30        
## [127] AnnotationFilter_1.29.0     bitops_1.0-8               
## [129] txdbmaker_1.1.1             VariantAnnotation_1.51.0   
## [131] scales_1.3.0                crayon_1.5.3               
## [133] rlang_1.1.4                 KEGGREST_1.45.1