crisprVerse: ecosystem of R packages for CRISPR gRNA design

Installation and getting started

The crisprVerse is a collection of packages for CRISPR guide RNA (gRNA) design that can easily be installed with the crisprVerse package. This provides a convenient way of downloading and installing all crisprVerse packages with a single R command.

The package can be installed from the Bioconductor devel branch using the following commands in an R session:

if (!requireNamespace("BiocManager", quietly = TRUE))
    install.packages("BiocManager")

BiocManager::install(version="devel")
BiocManager::install("crisprVerse")

The core crisprVerse includes the packages that are commonly used for gRNA design, and are attached when you attach the crisprVerse package:

library(crisprVerse)

You can check that all crisprVerse packages are up-to-date with the function crisprVerse_update().

Components

The following packages are installed and loaded with the crisprVerse package:

Reproducibility

sessionInfo()
## R version 4.6.0 (2026-04-24)
## Platform: x86_64-pc-linux-gnu
## Running under: Ubuntu 24.04.4 LTS
## 
## Matrix products: default
## BLAS:   /usr/lib/x86_64-linux-gnu/openblas-pthread/libblas.so.3 
## LAPACK: /usr/lib/x86_64-linux-gnu/openblas-pthread/libopenblasp-r0.3.26.so;  LAPACK version 3.12.0
## 
## locale:
##  [1] LC_CTYPE=en_US.UTF-8       LC_NUMERIC=C              
##  [3] LC_TIME=en_US.UTF-8        LC_COLLATE=en_US.UTF-8    
##  [5] LC_MONETARY=en_US.UTF-8    LC_MESSAGES=en_US.UTF-8   
##  [7] LC_PAPER=en_US.UTF-8       LC_NAME=C                 
##  [9] LC_ADDRESS=C               LC_TELEPHONE=C            
## [11] LC_MEASUREMENT=en_US.UTF-8 LC_IDENTIFICATION=C       
## 
## time zone: Etc/UTC
## tzcode source: system (glibc)
## 
## attached base packages:
## [1] stats     graphics  grDevices utils     datasets  methods   base     
## 
## other attached packages:
##  [1] crisprViz_1.15.0       crisprDesign_1.15.0    crisprScore_1.17.0    
##  [4] crisprScoreData_1.17.0 ExperimentHub_3.3.0    AnnotationHub_4.3.0   
##  [7] BiocFileCache_3.3.0    dbplyr_2.5.2           BiocGenerics_0.59.6   
## [10] generics_0.1.4         crisprBowtie_1.17.0    crisprBase_1.17.0     
## [13] crisprVerse_1.15.0     BiocStyle_2.41.0      
## 
## loaded via a namespace (and not attached):
##   [1] RColorBrewer_1.1-3          rstudioapi_0.18.0          
##   [3] sys_3.4.3                   jsonlite_2.0.0             
##   [5] magrittr_2.0.5              GenomicFeatures_1.65.0     
##   [7] farver_2.1.2                rmarkdown_2.31             
##   [9] BiocIO_1.23.3               vctrs_0.7.3                
##  [11] memoise_2.0.1               Rsamtools_2.29.0           
##  [13] RCurl_1.98-1.18             base64enc_0.1-6            
##  [15] htmltools_0.5.9             S4Arrays_1.13.0            
##  [17] BiocBaseUtils_1.15.1        progress_1.2.3             
##  [19] curl_7.1.0                  SparseArray_1.13.2         
##  [21] Formula_1.2-5               sass_0.4.10                
##  [23] bslib_0.11.0                htmlwidgets_1.6.4          
##  [25] Gviz_1.57.0                 httr2_1.2.2                
##  [27] cachem_1.1.0                buildtools_1.0.0           
##  [29] GenomicAlignments_1.49.0    lifecycle_1.0.5            
##  [31] pkgconfig_2.0.3             Matrix_1.7-5               
##  [33] R6_2.6.1                    fastmap_1.2.0              
##  [35] MatrixGenerics_1.25.0       digest_0.6.39              
##  [37] colorspace_2.1-2            AnnotationDbi_1.75.0       
##  [39] S4Vectors_0.51.3            Hmisc_5.2-5                
##  [41] GenomicRanges_1.65.0        RSQLite_3.53.1             
##  [43] filelock_1.0.3              randomForest_4.7-1.2       
##  [45] httr_1.4.8                  abind_1.4-8                
##  [47] compiler_4.6.0              Rbowtie_1.53.0             
##  [49] bit64_4.8.2                 backports_1.5.1            
##  [51] htmlTable_2.5.0             S7_0.2.2                   
##  [53] BiocParallel_1.47.0         DBI_1.3.0                  
##  [55] biomaRt_2.69.0              rappdirs_0.3.4             
##  [57] DelayedArray_0.39.3         rjson_0.2.23               
##  [59] tools_4.6.0                 foreign_0.8-91             
##  [61] nnet_7.3-20                 glue_1.8.1                 
##  [63] restfulr_0.0.16             grid_4.6.0                 
##  [65] checkmate_2.3.4             cluster_2.1.8.2            
##  [67] gtable_0.3.6                BSgenome_1.81.0            
##  [69] tzdb_0.5.0                  ensembldb_2.37.1           
##  [71] data.table_1.18.4           hms_1.1.4                  
##  [73] XVector_0.53.0              BiocVersion_3.24.0         
##  [75] pillar_1.11.1               stringr_1.6.0              
##  [77] dplyr_1.2.1                 lattice_0.22-9             
##  [79] deldir_2.0-4                rtracklayer_1.73.0         
##  [81] bit_4.6.0                   biovizBase_1.61.0          
##  [83] tidyselect_1.2.1            maketools_1.3.2            
##  [85] Biostrings_2.81.2           knitr_1.51                 
##  [87] gridExtra_2.3               ProtGenerics_1.45.0        
##  [89] IRanges_2.47.2              Seqinfo_1.3.0              
##  [91] SummarizedExperiment_1.43.0 stats4_4.6.0               
##  [93] xfun_0.57                   Biobase_2.73.1             
##  [95] matrixStats_1.5.0           stringi_1.8.7              
##  [97] UCSC.utils_1.9.0            lazyeval_0.2.3             
##  [99] yaml_2.3.12                 evaluate_1.0.5             
## [101] codetools_0.2-20            cigarillo_1.3.0            
## [103] interp_1.1-6                tibble_3.3.1               
## [105] BiocManager_1.30.27         cli_3.6.6                  
## [107] rpart_4.1.27                reticulate_1.46.0          
## [109] jquerylib_0.1.4             dichromat_2.0-0.1          
## [111] Rcpp_1.1.1-1.1              GenomeInfoDb_1.49.1        
## [113] png_0.1-9                   XML_3.99-0.23              
## [115] parallel_4.6.0              ggplot2_4.0.3              
## [117] readr_2.2.0                 blob_1.3.0                 
## [119] prettyunits_1.2.0           jpeg_0.1-11                
## [121] latticeExtra_0.6-31         AnnotationFilter_1.37.0    
## [123] bitops_1.0-9                txdbmaker_1.9.0            
## [125] VariantAnnotation_1.59.0    scales_1.4.0               
## [127] crayon_1.5.3                rlang_1.2.0                
## [129] KEGGREST_1.53.0