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  "Title": "A toolkit for single-cell immune receptor profiling",
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  "Description": "scRepertoire is a toolkit for processing and analyzing\nsingle-cell T-cell receptor (TCR) and immunoglobulin (Ig). The\nscRepertoire framework supports use of 10x, AIRR, BD, MiXCR,\nTRUST4, and WAT3R single-cell formats. The functionality\nincludes basic clonal analyses, repertoire summaries,\ndistance-based clustering and interaction with the popular\nSeurat and SingleCellExperiment/Bioconductor R single-cell\nworkflows.",
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      "concept": [
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      "title": "Scatter Plot Comparing Clones Across Two Samples",
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      "title": "Plot powerTCR Clustering Based on Clonal Size",
      "concept": [
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      "title": "Plot Relative Amino Acid Composition by Position",
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      "source": "vignette.Rmd",
      "filename": "vignette.html",
      "title": "Starting work with scRepertoire.",
      "author": "Nick Borcherding",
      "engine": "knitr::rmarkdown",
      "headings": [
        "Introduction",
        "Loading Libraries",
        "Loading Data",
        "What data to load into scRepertoire?",
        "Demonstrating Manual Data Loading (10x Genomics)",
        "Other alignment workflows",
        "Supported Formats and Expected File Names",
        "Multiplexed Experiment",
        "Important Considerations for createHTOContigList()",
        "Example Data in scRepertoire",
        "Combining Contigs into Clones",
        "combineBCR",
        "How combineBCR() Groups Related Clones",
        "Advanced: Grouping by Alignment",
        "Filtering and Cleaning Data",
        "Additional Processing",
        "addVariable: Adding Variables for Plotting",
        "subsetClones: Filter Out Clonal Information",
        "exportClones: Save Clonal Data",
        "clonalBin: Bin Clones by Frequency or Proportion",
        "Basic Usage with Proportion",
        "Using Frequency-Based Binning",
        "Grouping by a Variable",
        "annotateInvariant",
        "Basic Clonal Visualizations",
        "cloneCall: How to call clones.",
        "clonalQuant: Quantifying Unique Clones",
        "clonalAbundance: Distribution of Clones by Size",
        "clonalLength: Distribution of Sequence Lengths",
        "clonalCompare: Clonal Dynamics Between Categorical Variables",
        "clonalScatter: Scatterplot of Two Variables",
        "Visualizing Clonal Dynamics",
        "clonalHomeostasis: Examining Clonal Space",
        "clonalProportion: Examining Space Occupied by Ranks of Clones",
        "Summarizing Repertoires",
        "percentGeneUsage",
        "vizGenes: Flexible Gene Usage Visualization",
        "percentGenes: Quantifying Single Gene Usage",
        "percentVJ: Quantifying V-J Gene Pairings",
        "percentAA: Amino Acid Composition by Residue",
        "positionalEntropy: Entropy across CDR3 Sequences",
        "positionalProperty: Amino Acid Properties across CDR3 Sequence",
        "percentKmer: Motif Quantification",
        "Comparing Clonal Diversity and Overlap",
        "clonalDiversity: Clonal Diversity Quantification",
        "How clonalDiversity() Handles Downsampling and Bootstrapping",
        "Available Diversity Metrics (metric)",
        "clonalRarefaction: Sampling-based Extrapolation",
        "Hill Numbers and Their Interpretation",
        "plot.type Options",
        "Rarefaction using Species Richness (q = 0)",
        "Rarefaction using Shannon Diversity (q = 1)",
        "clonalSizeDistribution: Modeling Clonal Composition",
        "clonalOverlap: Exploring Sequence Overlap",
        "method Parameters for clonalOverlap()",
        "Combining Clones and Single-Cell Objects",
        "Preprocessed Single-Cell Object",
        "Note on Dimensional Reduction",
        "Functions to Exclude VDJ Genes",
        "combineExpression",
        "Calculating cloneSize",
        "Combining both TCR and BCR",
        "Visualizations for Single-Cell Objects",
        "clonalOverlay",
        "clonalNetwork",
        "Filtering Options for clonalNetwork()",
        "highlightClones",
        "clonalOccupy",
        "alluvialClones",
        "Basic Alluvial Plot",
        "Filtering to Top Clones",
        "Highlighting Specific Clones",
        "Visual Customization",
        "getCirclize and vizCirclize",
        "Quick Visualization with vizCirclize",
        "Manual Control with getCirclize",
        "Multi-Level Hierarchical Grouping",
        "Directional Flow",
        "Different Similarity Methods",
        "Subsetting and Proportion",
        "Quantifying Clonal Bias",
        "StartracDiversity",
        "Indices Output from StartracDiversity()",
        "Calculating a Single Index",
        "Pairwise Migration Analysis",
        "clonalBias",
        "Clustering by Edit Distance",
        "clonalCluster: Cluster by Sequence Similarity",
        "Core Concepts",
        "Understanding the threshold Parameter",
        "Demonstrating Basic Use",
        "Demonstrating Clustering with a Single-Cell Object",
        "Returning an igraph Object:",
        "Returning a Sparse Adjacency Matrix",
        "Using Both Chains",
        "Using Different Clustering Algorithms",
        "Conclusion",
        "Session Info"
      ],
      "created": "2020-01-18 19:24:11",
      "modified": "2026-04-03 17:04:32",
      "commits": 52
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