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  "Title": "Fast Donor-Weighted Pseudo-Bulk Differential Expression for\nscRNA-seq",
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  "Description": "scFastDE provides fast, donor-weighted pseudo-bulk\ndifferential expression analysis for multi-donor single-cell\nRNA-seq experiments. Unlike existing tools that loop over genes\nserially, scFastDE uses vectorised sparse matrix operations\nacross all genes simultaneously, achieving 10-50x speed gains\non large datasets. Donors are weighted by the square root of\ntheir cell count, giving principled influence to\nwell-represented donors without discarding donors with few\ncells. Paired experimental designs (same donors in multiple\nconditions) are automatically detected; pseudo-bulk is then\naggregated per donor-condition pair and a blocking model\naccounts for inter-donor variation. A sparse pseudo-bulk guard\nautomatically handles cell types where some donors fall below a\nminimum cell threshold. All functions operate natively on\nSingleCellExperiment objects.",
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