{
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  "Package": "abseqR",
  "Type": "Package",
  "Title": "Reporting and data analysis functionalities for Rep-Seq datasets\nof antibody libraries",
  "Version": "1.31.0",
  "Authors@R": "c(\nperson(\"JiaHong\", \"Fong\", email = \"jiahfong@gmail.com\", role = c(\"cre\", \"aut\")),\nperson(\"Monther\", \"Alhamdoosh\", email = \"m.hamdoosh@gmail.com\", role = \"aut\"))",
  "Description": "AbSeq is a comprehensive bioinformatic pipeline for the\nanalysis of sequencing datasets generated from antibody\nlibraries and abseqR is one of its packages. abseqR empowers\nthe users of abseqPy (https://github.com/malhamdoosh/abseqPy)\nwith plotting and reporting capabilities and allows them to\ngenerate interactive HTML reports for the convenience of\nviewing and sharing with other researchers. Additionally,\nabseqR extends abseqPy to compare multiple repertoire analyses\nand perform further downstream analysis on its output.",
  "License": "GPL-3 | file LICENSE",
  "Encoding": "UTF-8",
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  "Collate": "'accessors-AbSeq.R' 'AbSeqCRep.R' 'util.R' 'distributions.R'\n'upstreamAnalysis.R' 'productivityAnalysis.R'\n'primerAnalysis.R' 'diversityAnalysis.R' 'annotationAnalysis.R'\n'abundanceAnalysis.R' 'plotter.R' 'AbSeqRep.R' 'abseqReport.R'\n'statistics.R' 'pairwise.R'",
  "SystemRequirements": "pandoc (>= 1.19.2.1)",
  "URL": "https://github.com/malhamdoosh/abseqR",
  "BugReports": "https://github.com/malhamdoosh/abseqR/issues",
  "biocViews": "Sequencing, Visualization, ReportWriting, QualityControl,\nMultipleComparison",
  "Config/pak/sysreqs": "cmake make libicu-dev libpng-dev libuv1-dev\nlibssl-dev zlib1g-dev",
  "Repository": "https://bioc.r-universe.dev",
  "Date/Publication": "2026-04-28 12:49:07 UTC",
  "RemoteUrl": "https://github.com/bioc/abseqR",
  "RemoteRef": "HEAD",
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  "NeedsCompilation": "no",
  "Packaged": {
    "Date": "2026-05-29 08:50:57 UTC",
    "User": "root"
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  "Author": "JiaHong Fong [cre, aut],\nMonther Alhamdoosh [aut]",
  "Maintainer": "JiaHong Fong <jiahfong@gmail.com>",
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  "_published": "2026-05-29T10:59:14.044Z",
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    "reportwriting",
    "qualitycontrol",
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  "_assets": [
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    "extra/citation.html",
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    "extra/citation.txt",
    "extra/contents.json",
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    "manual.pdf"
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  "_homeurl": "https://github.com/malhamdoosh/abseqr",
  "_realowner": "bioc",
  "_cranurl": false,
  "_exports": [
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    "report"
  ],
  "_help": [
    {
      "page": "dot-abundanceAnalysis",
      "title": "Conducts abundance analysis",
      "topics": [
        ".abundanceAnalysis"
      ]
    },
    {
      "page": "dot-abundancePlot",
      "title": "Abundance distribution",
      "topics": [
        ".abundancePlot"
      ]
    },
    {
      "page": "dot-alignQualityHeatMaps",
      "title": "Plots all 5 alignment quality heatmaps",
      "topics": [
        ".alignQualityHeatMaps"
      ]
    },
    {
      "page": "dot-allPrimerNames",
      "title": "Collect primer names from FASTA",
      "topics": [
        ".allPrimerNames"
      ]
    },
    {
      "page": "dot-aminoAcidBar",
      "title": "Plots amino acid composition logo",
      "topics": [
        ".aminoAcidBar"
      ]
    },
    {
      "page": "dot-aminoAcidPlot",
      "title": "Composition logo plot",
      "topics": [
        ".aminoAcidPlot"
      ]
    },
    {
      "page": "dot-analyzeUpstreamValidity",
      "title": "Plots the validity of upstream sequences",
      "topics": [
        ".analyzeUpstreamValidity"
      ]
    },
    {
      "page": "dot-annotAnalysis",
      "title": "Annotation analysis",
      "topics": [
        ".annotAnalysis"
      ]
    },
    {
      "page": "dot-asRepertoireAlignLen",
      "title": "Accessor for 'alignlen' slot",
      "topics": [
        ".asRepertoireAlignLen"
      ]
    },
    {
      "page": "dot-asRepertoireBitscore",
      "title": "Accessor for 'bitscore' slot",
      "topics": [
        ".asRepertoireBitscore"
      ]
    },
    {
      "page": "dot-asRepertoireChain",
      "title": "Accessor for 'chain' slot",
      "topics": [
        ".asRepertoireChain"
      ]
    },
    {
      "page": "dot-asRepertoireDir",
      "title": "Accessor for the 'outdir' slot",
      "topics": [
        ".asRepertoireDir"
      ]
    },
    {
      "page": "dot-asRepertoireList",
      "title": "Accessor for AbSeqCRep's list of AbSeqRep objects",
      "topics": [
        ".asRepertoireList"
      ]
    },
    {
      "page": "dot-asRepertoireName",
      "title": "Accessor for the 'name' slot",
      "topics": [
        ".asRepertoireName"
      ]
    },
    {
      "page": "dot-asRepertoirePrimer3",
      "title": "Accessor for the 'primer3end' slot",
      "topics": [
        ".asRepertoirePrimer3"
      ]
    },
    {
      "page": "dot-asRepertoirePrimer5",
      "title": "Accessor for the 'primer5end' slot",
      "topics": [
        ".asRepertoirePrimer5"
      ]
    },
    {
      "page": "dot-asRepertoireQueryStart",
      "title": "Accessor for 'qstart' slot",
      "topics": [
        ".asRepertoireQueryStart"
      ]
    },
    {
      "page": "dot-asRepertoireSubjectStart",
      "title": "Accessor for 'sstart' slot",
      "topics": [
        ".asRepertoireSubjectStart"
      ]
    },
    {
      "page": "dot-asRepertoireUpstream",
      "title": "Accessor for the 'upstream' slot",
      "topics": [
        ".asRepertoireUpstream"
      ]
    },
    {
      "page": "dot-boxPlot",
      "title": "Creates a box plot",
      "topics": [
        ".boxPlot"
      ]
    },
    {
      "page": "dot-calculateDInd",
      "title": "Calculates the \"standard\" diversity indices",
      "topics": [
        ".calculateDInd"
      ]
    },
    {
      "page": "dot-calculateDiversityEstimates",
      "title": "Calculates Lower Bound Estimates for unseen species and Common Diversity Indices from clonotype tables",
      "topics": [
        ".calculateDiversityEstimates"
      ]
    },
    {
      "page": "dot-canonicalizeTitle",
      "title": "Convert file names to human friendly text",
      "topics": [
        ".canonicalizeTitle"
      ]
    },
    {
      "page": "dot-capitalize",
      "title": "Helper function to capitalize the first letter of 'str'",
      "topics": [
        ".capitalize"
      ]
    },
    {
      "page": "dot-checkVert",
      "title": "Checks if abseqPy has a metadata line that suggests the orientation",
      "topics": [
        ".checkVert"
      ]
    },
    {
      "page": "dot-cloneDistHist",
      "title": "Marginal histogram of clonotypes (blue for shared, grey for total). The y axis is scaled by sqrt (but it doesn't really matter anyway, since we're stripping away the y-ticks)",
      "topics": [
        ".cloneDistHist"
      ]
    },
    {
      "page": "dot-cloneDistMarginal",
      "title": "Marginal density graph of clonotypes (blue for shared, grey for total, purple for exclusive clones)",
      "topics": [
        ".cloneDistMarginal"
      ]
    },
    {
      "page": "dot-clonotypeAnalysis",
      "title": "Comprehensive clonotype analyses",
      "topics": [
        ".clonotypeAnalysis"
      ]
    },
    {
      "page": "dot-collateReports",
      "title": "Collate all HTML reports into a single directory and cretate an entry 'index.html' file that redirects to all collated HTML files",
      "topics": [
        ".collateReports"
      ]
    },
    {
      "page": "dot-commonPrimerNames",
      "title": "Collect the intersection of all primer names within a collection of primer files",
      "topics": [
        ".commonPrimerNames"
      ]
    },
    {
      "page": "dot-correlationTest",
      "title": "Conducts pearson and spearman correlation analysis on dataframe",
      "topics": [
        ".correlationTest"
      ]
    },
    {
      "page": "dot-distanceMeasure",
      "title": "Computes the distance between pariwise samples",
      "topics": [
        ".distanceMeasure"
      ]
    },
    {
      "page": "dot-diversityAnalysis",
      "title": "Title Diversity analysis",
      "topics": [
        ".diversityAnalysis"
      ]
    },
    {
      "page": "dot-emptyPlot",
      "title": "Creates and returns an empty plot",
      "topics": [
        ".emptyPlot"
      ]
    },
    {
      "page": "dot-findRepertoires",
      "title": "Given a directory = <abseqPy_outputdir>/RESULT_DIR/, returns the directories (repositories) in 'directory'. That is, will not return any sample_vs_sample directories. This is done by asserting that a 'repository' must have an (analysis.params) file, and a summary.txt file.",
      "topics": [
        ".findRepertoires"
      ]
    },
    {
      "page": "dot-generateAllSpectratypes",
      "title": "Generates all FR/CDR spectratypes",
      "topics": [
        ".generateAllSpectratypes"
      ]
    },
    {
      "page": "dot-generateDelayedReport",
      "title": "Generates report for all samples in 'compare'",
      "topics": [
        ".generateDelayedReport"
      ]
    },
    {
      "page": "dot-generateReport",
      "title": "Generates HTML report from 'AbSeqRep' and 'AbSeqCRep' ojects",
      "topics": [
        ".generateReport"
      ]
    },
    {
      "page": "dot-getLineTypes",
      "title": "Helper function to return line types by importance based on provided CD/Fs regions",
      "topics": [
        ".getLineTypes"
      ]
    },
    {
      "page": "dot-getTotal",
      "title": "Get total number of samples (n)",
      "topics": [
        ".getTotal"
      ]
    },
    {
      "page": "dot-hmFromMatrix",
      "title": "Plots a plotly heatmap from provided matrix",
      "topics": [
        ".hmFromMatrix"
      ]
    },
    {
      "page": "dot-inferAnalyzed",
      "title": "Returns all samples found under 'sampleDirectory'",
      "topics": [
        ".inferAnalyzed"
      ]
    },
    {
      "page": "dot-loadMatrixFromDF",
      "title": "Given a dataframe with the columns \"from\", \"to\", and value.var, return a symmetric matrix (with diagonal values = diag). I.e. a call to isSymmetric(return_value_of_this_function) will always be TRUE.",
      "topics": [
        ".loadMatrixFromDF"
      ]
    },
    {
      "page": "dot-loadSamplesFromString",
      "title": "Loads AbSeqCRep or AbSeqRep objects from a list of sampleNames",
      "topics": [
        ".loadSamplesFromString"
      ]
    },
    {
      "page": "dot-pairwiseComparison",
      "title": "Conduct all vs all pairwise comparison analyses",
      "topics": [
        ".pairwiseComparison"
      ]
    },
    {
      "page": "dot-plotCirclize",
      "title": "V-J association plot",
      "topics": [
        ".plotCirclize"
      ]
    },
    {
      "page": "dot-plotDist",
      "title": "Bar plotter",
      "topics": [
        ".plotDist"
      ]
    },
    {
      "page": "dot-plotDiversityCurves",
      "title": "Plots rarefaction, recapture, and de-dup plots for specified 'region'",
      "topics": [
        ".plotDiversityCurves"
      ]
    },
    {
      "page": "dot-plotDuplication",
      "title": "Duplication level plot",
      "topics": [
        ".plotDuplication"
      ]
    },
    {
      "page": "dot-plotErrorDist",
      "title": "Plots the error distribution for each region: CDRs, FRs, IGV, IGD, and IGJ",
      "topics": [
        ".plotErrorDist"
      ]
    },
    {
      "page": "dot-plotIGVErrors",
      "title": "Plots the error distribution for IGV germlines",
      "topics": [
        ".plotIGVErrors"
      ]
    },
    {
      "page": "dot-plotIGVUpstreamLenDist",
      "title": "Plot IGV family distribution for a given 'upstreamLengthRange'",
      "topics": [
        ".plotIGVUpstreamLenDist"
      ]
    },
    {
      "page": "dot-plotIGVUpstreamLenDistDetailed",
      "title": "Plots the detailed length distribution for IGV families",
      "topics": [
        ".plotIGVUpstreamLenDistDetailed"
      ]
    },
    {
      "page": "dot-plotPrimerIGVStatus",
      "title": "Plots, for a given 'category' and 'pend', the 'primer' IGV indelled distribution in a bar plot",
      "topics": [
        ".plotPrimerIGVStatus"
      ]
    },
    {
      "page": "dot-plotPrimerIntegrity",
      "title": "Plots the distribution of primer integrity for a given 'category' and 5' or 3' 'pend'",
      "topics": [
        ".plotPrimerIntegrity"
      ]
    },
    {
      "page": "dot-plotRarefaction",
      "title": "Rarefaction plot",
      "topics": [
        ".plotRarefaction"
      ]
    },
    {
      "page": "dot-plotRecapture",
      "title": "Plots capture-recapture",
      "topics": [
        ".plotRecapture"
      ]
    },
    {
      "page": "dot-plotSamples",
      "title": "Monolith AbSeq Plot function - the \"driver\" program",
      "topics": [
        ".plotSamples"
      ]
    },
    {
      "page": "dot-plotSpectratype",
      "title": "Spectratype plotter",
      "topics": [
        ".plotSpectratype"
      ]
    },
    {
      "page": "dot-plotUpstreamLength",
      "title": "Plot upstream distribution",
      "topics": [
        ".plotUpstreamLength"
      ]
    },
    {
      "page": "dot-plotUpstreamLengthDist",
      "title": "Plot upstream sequence length distribution for upstream sequences (5'UTR or secretion signal) for a given 'upstreamLengthRange'",
      "topics": [
        ".plotUpstreamLengthDist"
      ]
    },
    {
      "page": "dot-primerAnalysis",
      "title": "Conducts primer specificity analysis",
      "topics": [
        ".primerAnalysis"
      ]
    },
    {
      "page": "dot-prodDistPlot",
      "title": "Plots a distribution plot for different productivity analysis files",
      "topics": [
        ".prodDistPlot"
      ]
    },
    {
      "page": "dot-productivityAnalysis",
      "title": "Conducts productivty analysis",
      "topics": [
        ".productivityAnalysis"
      ]
    },
    {
      "page": "dot-productivityPlot",
      "title": "Summary of productivity",
      "topics": [
        ".productivityPlot"
      ]
    },
    {
      "page": "dot-readSummary",
      "title": "Return value specifed by key from AbSeq's summary file",
      "topics": [
        ".readSummary"
      ]
    },
    {
      "page": "dot-regionAnalysis",
      "title": "Title Shows varying regions for a given clonotype defined by its CDR3",
      "topics": [
        ".regionAnalysis"
      ]
    },
    {
      "page": "dot-reportLBE",
      "title": "Reports abundance-based (Lower bound) diversity estimates using the Vegan package",
      "topics": [
        ".reportLBE"
      ]
    },
    {
      "page": "dot-saveAs",
      "title": "Saves ggplot object as a Rdata file.",
      "topics": [
        ".saveAs"
      ]
    },
    {
      "page": "dot-scatterPlot",
      "title": "Title Creates a scatter plot",
      "topics": [
        ".scatterPlot"
      ]
    },
    {
      "page": "dot-scatterPlotComplex",
      "title": "Creates a complex scatter plot",
      "topics": [
        ".scatterPlotComplex"
      ]
    },
    {
      "page": "dot-secretionSignalAnalysis",
      "title": "Secretion signal analysis",
      "topics": [
        ".secretionSignalAnalysis"
      ]
    },
    {
      "page": "dot-substituteStringInFile",
      "title": "Substitutes the first occurance of `key` with `value` in `filename`",
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      "page": "dot-summarySE",
      "title": "Summary of dataframe",
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    {
      "page": "dot-topNDist",
      "title": "Title Clonotype table",
      "topics": [
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    },
    {
      "page": "dot-UTR5Analysis",
      "title": "5' UTR analysis",
      "topics": [
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    },
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      "page": "dot-vennIntersection",
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      "topics": [
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    },
    {
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      "title": "Combines 2 AbSeqCRep objects together for comparison",
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      "page": "abseqReport",
      "title": "Visualize all analysis conducted by abseqPy",
      "topics": [
        "abseqReport"
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    },
    {
      "page": "report",
      "title": "Plots AbSeqRep or AbSeqCRep object to the specfied directory",
      "topics": [
        "report",
        "report,AbSeqCRep-method",
        "report,AbSeqRep-method"
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    }
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      "source": "abseqR.Rmd",
      "filename": "abseqR.html",
      "title": "abseqR: reporting and data analysis functionalities for Rep-Seq datasets of antibody libraries",
      "author": "Jia Hong Fong, Monther Alhamdoosh",
      "engine": "knitr::rmarkdown",
      "headings": [
        "Introduction",
        "AbSeq core analyses",
        "Installation",
        "Bioconductor",
        "GitHub",
        "System prerequisites",
        "R package dependencies",
        "Quick start",
        "Datasets",
        "Basic analysis",
        "HTML reports' directory structure",
        "Comparative analysis using multiple datasets",
        "Advanced Examples",
        "Lazy loading",
        "Alternative reporting options",
        "Parallelization",
        "Interpretation of report's figures",
        "Sequence quality analysis",
        "Sequence length",
        "Alignment quality",
        "Insertions, deletions, and mismatches",
        "Abundance and association analysis",
        "V-(D)-J germline abundance",
        "V-J germline associations",
        "Productivity analysis",
        "V-J frameshifts",
        "Stop codons",
        "Indels and mismatches",
        "Diversity analysis",
        "Clonotype-based analysis",
        "Spectratype analysis",
        "Position-specific analysis",
        "Comparative analysis",
        "Over-representation analysis",
        "Overlapping analysis",
        "Multi-sample analysis",
        "Two-sample analysis",
        "Correlation analysis",
        "Clustering analysis",
        "Appendices",
        "Datasets",
        "Session Info",
        "References"
      ],
      "created": "2018-07-27 03:10:01",
      "modified": "2018-10-24 05:20:41",
      "commits": 21
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