{
  "_id": "6a12c187acfb0bcc41d1deb7",
  "Package": "R453Plus1Toolbox",
  "Type": "Package",
  "Title": "A package for importing and analyzing data from Roche's Genome\nSequencer System",
  "Version": "1.63.0",
  "Date": "2026-04-05",
  "Author": "Hans-Ulrich Klein, Christoph Bartenhagen, Christian Ruckert",
  "Maintainer": "Hans-Ulrich Klein <h.klein@uni-muenster.de>",
  "Description": "The R453Plus1 Toolbox comprises useful functions for the\nanalysis of data generated by Roche's 454 sequencing platform.\nIt adds functions for quality assurance as well as for\nannotation and visualization of detected variants,\ncomplementing the software tools shipped by Roche with their\nproduct. Further, a pipeline for the detection of structural\nvariants is provided.",
  "License": "LGPL-3",
  "biocViews": "Sequencing, Infrastructure, DataImport, DataRepresentation,\nVisualization, QualityControl, ReportWriting",
  "Repository": "https://bioc.r-universe.dev",
  "Date/Publication": "2026-04-28 12:34:56 UTC",
  "RemoteUrl": "https://github.com/bioc/R453Plus1Toolbox",
  "RemoteRef": "HEAD",
  "RemoteSha": "8d8c63f53162b2fabd10980c3ac7e77f71e31e9e",
  "NeedsCompilation": "yes",
  "Packaged": {
    "Date": "2026-04-30 15:29:03 UTC",
    "User": "root"
  },
  "MD5sum": "3f91f3538da19d08e001d13766f45fd6",
  "_user": "bioc",
  "_type": "src",
  "_file": "R453Plus1Toolbox_1.63.0.tar.gz",
  "_fileid": "f8a8520d0c0bed145e3a3d6779d2602da2ee4c1a035de9d84facfdd6da0f6a70",
  "_filesize": 1206967,
  "_sha256": "f8a8520d0c0bed145e3a3d6779d2602da2ee4c1a035de9d84facfdd6da0f6a70",
  "_created": "2026-04-30T15:29:03.000Z",
  "_jobs": [
    {
      "job": 77586039273,
      "time": 462,
      "config": "bioc-checks",
      "r": "4.6.0",
      "check": "ERROR",
      "artifact": "6734164736"
    },
    {
      "job": 77586039369,
      "time": 588,
      "config": "linux-devel-arm64",
      "r": "4.7.0",
      "check": "ERROR",
      "artifact": "6734112618"
    },
    {
      "job": 77586039267,
      "time": 765,
      "config": "linux-devel-x86_64",
      "r": "4.7.0",
      "check": "NOTE",
      "artifact": "6734276819"
    },
    {
      "job": 77586039461,
      "time": 513,
      "config": "linux-release-arm64",
      "r": "4.6.0",
      "check": "NOTE",
      "artifact": "6734083531"
    },
    {
      "job": 77586039394,
      "time": 639,
      "config": "linux-release-x86_64",
      "r": "4.6.0",
      "check": "NOTE",
      "artifact": "6734231026"
    },
    {
      "job": 77586039424,
      "time": 408,
      "config": "macos-oldrel-arm64",
      "r": "4.5.3",
      "check": "NOTE",
      "artifact": "6736055749"
    },
    {
      "job": 77586039387,
      "time": 936,
      "config": "macos-oldrel-x86_64",
      "r": "4.5.3",
      "check": "NOTE",
      "artifact": "6735076201"
    },
    {
      "job": 77586039373,
      "time": 465,
      "config": "macos-release-arm64",
      "r": "4.6.0",
      "check": "NOTE",
      "artifact": "6736072016"
    },
    {
      "job": 77586039285,
      "time": 1021,
      "config": "macos-release-x86_64",
      "r": "4.6.0",
      "check": "NOTE",
      "artifact": "6735100574"
    },
    {
      "job": 77586039237,
      "time": 1056,
      "config": "source",
      "r": "4.6.0",
      "check": "OK",
      "artifact": "6733892212"
    },
    {
      "job": 77586039128,
      "time": 238,
      "config": "wasm-release",
      "r": "4.6.0",
      "check": "FAIL",
      "artifact": ""
    },
    {
      "job": 77586039291,
      "time": 594,
      "config": "windows-devel",
      "r": "4.7.0",
      "check": "NOTE",
      "artifact": "6734210861"
    },
    {
      "job": 77586039393,
      "time": 606,
      "config": "windows-oldrel",
      "r": "4.5.3",
      "check": "NOTE",
      "artifact": "6734225759"
    },
    {
      "job": 77586039304,
      "time": 579,
      "config": "windows-release",
      "r": "4.6.0",
      "check": "NOTE",
      "artifact": "6734205140"
    }
  ],
  "_bioccheck": {
    "error": 3,
    "warning": 3,
    "note": 19
  },
  "_buildurl": "https://github.com/r-universe/bioc/actions/runs/25173438673",
  "_status": "success",
  "_host": "GitHub-Actions",
  "_upstream": "https://github.com/bioc/R453Plus1Toolbox",
  "_commit": {
    "id": "8d8c63f53162b2fabd10980c3ac7e77f71e31e9e",
    "author": "A Wokaty <andres.wokaty@sph.cuny.edu>",
    "committer": "A Wokaty <andres.wokaty@sph.cuny.edu>",
    "message": "bump x.y.z version to odd y following creation of RELEASE_3_23 branch\n",
    "time": 1777379696
  },
  "_maintainer": {
    "name": "Hans-Ulrich Klein",
    "email": "h.klein@uni-muenster.de"
  },
  "_distro": "noble",
  "_registered": true,
  "_dependencies": [
    {
      "package": "R",
      "version": ">= 2.12.0",
      "role": "Depends"
    },
    {
      "package": "methods",
      "role": "Depends"
    },
    {
      "package": "VariantAnnotation",
      "version": ">= 1.25.11",
      "role": "Depends"
    },
    {
      "package": "Biostrings",
      "version": ">= 2.47.6",
      "role": "Depends"
    },
    {
      "package": "pwalign",
      "role": "Depends"
    },
    {
      "package": "Biobase",
      "role": "Depends"
    },
    {
      "package": "utils",
      "role": "Imports"
    },
    {
      "package": "grDevices",
      "role": "Imports"
    },
    {
      "package": "graphics",
      "role": "Imports"
    },
    {
      "package": "stats",
      "role": "Imports"
    },
    {
      "package": "tools",
      "role": "Imports"
    },
    {
      "package": "xtable",
      "role": "Imports"
    },
    {
      "package": "R2HTML",
      "role": "Imports"
    },
    {
      "package": "TeachingDemos",
      "role": "Imports"
    },
    {
      "package": "BiocGenerics",
      "role": "Imports"
    },
    {
      "package": "S4Vectors",
      "version": ">= 0.17.25",
      "role": "Imports"
    },
    {
      "package": "IRanges",
      "version": ">= 2.13.12",
      "role": "Imports"
    },
    {
      "package": "XVector",
      "role": "Imports"
    },
    {
      "package": "GenomicRanges",
      "version": ">= 1.31.8",
      "role": "Imports"
    },
    {
      "package": "SummarizedExperiment",
      "role": "Imports"
    },
    {
      "package": "biomaRt",
      "role": "Imports"
    },
    {
      "package": "BSgenome",
      "version": ">= 1.47.3",
      "role": "Imports"
    },
    {
      "package": "Rsamtools",
      "role": "Imports"
    },
    {
      "package": "ShortRead",
      "version": ">= 1.37.1",
      "role": "Imports"
    },
    {
      "package": "rtracklayer",
      "role": "Suggests"
    },
    {
      "package": "BSgenome.Hsapiens.UCSC.hg19",
      "role": "Suggests"
    },
    {
      "package": "BSgenome.Scerevisiae.UCSC.sacCer2",
      "role": "Suggests"
    }
  ],
  "_owner": "bioc",
  "_selfowned": true,
  "_usedby": 0,
  "_updates": [
    {
      "week": "2025-44",
      "n": 2
    },
    {
      "week": "2026-14",
      "n": 1
    },
    {
      "week": "2026-18",
      "n": 2
    }
  ],
  "_tags": [],
  "_bioc": [
    {
      "branch": "devel",
      "version": "1.63.0",
      "bioc": "3.24"
    },
    {
      "branch": "release",
      "version": "1.62.0",
      "bioc": "3.23"
    }
  ],
  "_topics": [
    "sequencing",
    "infrastructure",
    "dataimport",
    "datarepresentation",
    "visualization",
    "qualitycontrol",
    "reportwriting"
  ],
  "_userbio": {
    "uuid": 2286807,
    "type": "organization",
    "name": "Bioconductor",
    "description": "Software for the analysis and comprehension of high-throughput genomic data"
  },
  "_downloads": {
    "count": 410,
    "source": "https://www.bioconductor.org/packages/stats/bioc/R453Plus1Toolbox"
  },
  "_mentions": 1,
  "_searchresults": 10,
  "_rbuild": "4.6.0",
  "_assets": [
    "extra/citation.cff",
    "extra/citation.html",
    "extra/citation.json",
    "extra/citation.txt",
    "extra/contents.json",
    "extra/NEWS.html",
    "extra/NEWS.txt",
    "extra/R453Plus1Toolbox.html",
    "manual.pdf"
  ],
  "_realowner": "bioc",
  "_cranurl": false,
  "_exports": [
    "addRead",
    "alignedReadsC1",
    "alignedReadsC1<-",
    "alignedReadsC2",
    "alignedReadsC2<-",
    "alignShortReads",
    "annotatedVariants",
    "annotateVariants",
    "assayDataAmp",
    "assayDataAmp<-",
    "ava2vcf",
    "AVASet",
    "baseFrequency",
    "baseQualityHist",
    "baseQualityStats",
    "calculateTiTv",
    "clipAdapterLeft",
    "clipAdapterLeft<-",
    "clipAdapterRight",
    "clipAdapterRight<-",
    "clipQualityLeft",
    "clipQualityLeft<-",
    "clipQualityRight",
    "clipQualityRight<-",
    "commonAlignC1",
    "commonAlignC1<-",
    "commonAlignC2",
    "commonAlignC2<-",
    "commonBpsC1",
    "commonBpsC1<-",
    "commonBpsC2",
    "commonBpsC2<-",
    "complexity.dust",
    "complexity.entropy",
    "coverageOnTarget",
    "demultiplexReads",
    "detectBreakpoints",
    "dinucleotideOddsRatio",
    "extendedCIGARToList",
    "fDataAmp",
    "featureDataAmp",
    "featureDataAmp<-",
    "filterChimericReads",
    "flowChars",
    "flowChars<-",
    "flowgram",
    "flowgram<-",
    "flowgramBarplot",
    "flowgramFormat",
    "flowgramFormat<-",
    "flowgrams",
    "flowgrams<-",
    "flowIndexes",
    "flowIndexes<-",
    "gcContent",
    "gcContentHist",
    "gcPerPosition",
    "genomeSequencerMIDs",
    "getAlignedReads",
    "getRead",
    "getReadStatus",
    "getVariantPercentages",
    "homopolymerHist",
    "htmlReport",
    "keySequence",
    "keySequence<-",
    "listToExtendedCIGAR",
    "MapperSet",
    "mergeBreakpoints",
    "name",
    "name<-",
    "nucleotideCharts",
    "plotAmpliconCoverage",
    "plotChimericReads",
    "plotVariants",
    "plotVariationFrequency",
    "positionQualityBoxplot",
    "quality",
    "quality<-",
    "qualityReportSFF",
    "read",
    "read<-",
    "readLengthHist",
    "readLengthStats",
    "reads",
    "reads<-",
    "readSFF",
    "readsOnTarget",
    "referenceSequences",
    "referenceSequences<-",
    "removeLinker",
    "seqsC1",
    "seqsC1<-",
    "seqsC2",
    "seqsC2<-",
    "sequenceCaptureLinkers",
    "sequenceQualityHist",
    "setVariantFilter",
    "sff2fastq",
    "writeSFF"
  ],
  "_datasets": [
    {
      "name": "avaSetExample",
      "title": "Amplicon Variant Analyzer data import",
      "object": "avaSetExample",
      "file": "avaSetExample.RData",
      "class": [
        "AVASet"
      ],
      "fields": [],
      "table": false,
      "tojson": false
    },
    {
      "name": "avaSetFiltered",
      "title": "Amplicon Variant Analyzer data import",
      "object": "avaSetFiltered",
      "file": "avaSetFiltered.RData",
      "class": [
        "AVASet"
      ],
      "fields": [],
      "table": false,
      "tojson": false
    },
    {
      "name": "avaSetFiltered_annot",
      "title": "AVASet variant annotations",
      "object": "avaSetFiltered_annot",
      "file": "avaSetFiltered_annot.RData",
      "class": [
        "AnnotatedVariants"
      ],
      "fields": [],
      "table": false,
      "tojson": false
    },
    {
      "name": "breakpoints",
      "title": "Putative breakpoints of chimeric reads",
      "object": "breakpoints",
      "file": "breakpoints.RData",
      "class": [
        "Breakpoints"
      ],
      "fields": [],
      "table": false,
      "tojson": false
    },
    {
      "name": "captureArray",
      "title": "Custom capture array design",
      "object": "captureArray",
      "file": "captureArray.RData",
      "class": [
        "CompressedIRangesList"
      ],
      "fields": [],
      "table": false,
      "tojson": false
    },
    {
      "name": "mapperSetExample",
      "title": "GS Reference Mapper data import",
      "object": "mapperSetExample",
      "file": "mapperSetExample.RData",
      "class": [
        "MapperSet"
      ],
      "fields": [],
      "table": false,
      "tojson": false
    },
    {
      "name": "mutationInfo",
      "title": "Example data for 'plotVariants'",
      "object": "plotVariantsExample",
      "file": "plotVariantsExample.RData",
      "class": [
        "data.frame"
      ],
      "fields": [
        "mutation",
        "legend",
        "color"
      ],
      "rows": 4,
      "table": true,
      "tojson": true
    },
    {
      "name": "regions",
      "title": "Example data for 'plotVariants'",
      "object": "plotVariantsExample",
      "file": "plotVariantsExample.RData",
      "class": [
        "data.frame"
      ],
      "fields": [
        "name",
        "start",
        "end",
        "color"
      ],
      "rows": 2,
      "table": true,
      "tojson": true
    },
    {
      "name": "variants",
      "title": "Example data for 'plotVariants'",
      "object": "plotVariantsExample",
      "file": "plotVariantsExample.RData",
      "class": [
        "data.frame"
      ],
      "fields": [
        "label",
        "pos",
        "mutation",
        "color"
      ],
      "rows": 4,
      "table": true,
      "tojson": true
    }
  ],
  "_help": [
    {
      "page": "alignShortReads",
      "title": "Exact alignment of DNA sequences against a reference",
      "topics": [
        "alignShortReads",
        "alignShortReads,AVASet,BSgenome,character,logical-method",
        "alignShortReads,AVASet,BSgenome,character,missing-method",
        "alignShortReads,AVASet,BSgenome,missing,logical-method",
        "alignShortReads,AVASet,BSgenome,missing,missing-method",
        "alignShortReads,DNAStringSet,BSgenome,character,logical-method",
        "alignShortReads,DNAStringSet,BSgenome,character,missing-method",
        "alignShortReads,DNAStringSet,BSgenome,missing,logical-method",
        "alignShortReads,DNAStringSet,BSgenome,missing,missing-method"
      ]
    },
    {
      "page": "AnnotatedVariants-class",
      "title": "Class \"AnnotatedVariants\"",
      "topics": [
        "annotatedVariants",
        "annotatedVariants,AnnotatedVariants-method",
        "AnnotatedVariants-class",
        "annotatedVariants<-,AnnotatedVariants,list-method",
        "names,AnnotatedVariants-method",
        "names<-,AnnotatedVariants,character-method"
      ]
    },
    {
      "page": "annotateVariants",
      "title": "Adds genomic information to variants",
      "topics": [
        "annotateVariants",
        "annotateVariants,AVASet,missing-method",
        "annotateVariants,data.frame,missing-method",
        "annotateVariants,MapperSet,BSgenome-method",
        "annotateVariants,MapperSet,missing-method"
      ]
    },
    {
      "page": "assayDataAmp",
      "title": "Access the amplicon data of an AVASet.",
      "topics": [
        "assayDataAmp"
      ]
    },
    {
      "page": "ava2vcf",
      "title": "Convert an AVASet object into a VCF object",
      "topics": [
        "ava2vcf",
        "ava2vcf,AVASet-method"
      ]
    },
    {
      "page": "AVASet",
      "title": "Creating an AVASet",
      "topics": [
        "AVASet",
        "AVASet,character,character,missing,missing,missing,missing,missing-method",
        "AVASet,character,missing,character,character,character,character,character-method",
        "AVASet,character,missing,character,character,character,missing,missing-method",
        "AVASet,character,missing,missing,missing,missing,missing,missing-method"
      ]
    },
    {
      "page": "AVASet-class",
      "title": "Class to contain Amplicon Variant Analyzer Output",
      "topics": [
        "alignShortReads,AVASet,DNAStringSet,character-method",
        "annotateVariants,AVASet-method",
        "assayDataAmp,AVASet-method",
        "assayDataAmp<-",
        "assayDataAmp<-,AVASet,AssayData-method",
        "AVASet-class",
        "fDataAmp,AVASet-method",
        "featureDataAmp,AVASet-method",
        "featureDataAmp<-",
        "featureDataAmp<-,AVASet,AnnotatedDataFrame-method",
        "getVariantPercentages,AVASet-method",
        "htmlReport,AVASet-method",
        "referenceSequences,AVASet-method",
        "referenceSequences<-",
        "referenceSequences<-,AVASet,AlignedRead-method",
        "setVariantFilter,AVASet-method",
        "[,AVASet,ANY,ANY,ANY-method",
        "[,AVASet,ANY,ANY-method"
      ]
    },
    {
      "page": "avaSetExample",
      "title": "Amplicon Variant Analyzer data import",
      "topics": [
        "avaSetExample"
      ]
    },
    {
      "page": "avaSetFiltered",
      "title": "Amplicon Variant Analyzer data import",
      "topics": [
        "avaSetFiltered"
      ]
    },
    {
      "page": "avaSetFiltered_annot",
      "title": "AVASet variant annotations",
      "topics": [
        "avaSetFiltered_annot"
      ]
    },
    {
      "page": "baseFrequency",
      "title": "Absolute And Relative Frequency Of The Four Bases.",
      "topics": [
        "baseFrequency",
        "baseFrequency,DNAStringSet-method",
        "baseFrequency,SFFContainer-method",
        "baseFrequency,ShortRead-method"
      ]
    },
    {
      "page": "baseQualityHist",
      "title": "Plot A Histogram Of The Base Qualities.",
      "topics": [
        "baseQualityHist",
        "baseQualityHist,QualityScaledDNAStringSet-method",
        "baseQualityHist,SFFContainer-method",
        "baseQualityHist,ShortReadQ-method"
      ]
    },
    {
      "page": "baseQualityStats",
      "title": "Statistics Of Base Quality",
      "topics": [
        "baseQualityStats",
        "baseQualityStats,QualityScaledDNAStringSet-method",
        "baseQualityStats,SFFContainer-method",
        "baseQualityStats,ShortReadQ-method"
      ]
    },
    {
      "page": "breakpoints",
      "title": "Putative breakpoints of chimeric reads",
      "topics": [
        "breakpoints"
      ]
    },
    {
      "page": "Breakpoints-class",
      "title": "Class \"Breakpoints\"",
      "topics": [
        "alignedReadsC1",
        "alignedReadsC1,Breakpoints-method",
        "alignedReadsC1<-",
        "alignedReadsC1<-,Breakpoints,list-method",
        "alignedReadsC2",
        "alignedReadsC2,Breakpoints-method",
        "alignedReadsC2<-",
        "alignedReadsC2<-,Breakpoints,list-method",
        "Breakpoints-class",
        "commonAlignC1",
        "commonAlignC1,Breakpoints-method",
        "commonAlignC1<-",
        "commonAlignC1<-,Breakpoints,list-method",
        "commonAlignC2",
        "commonAlignC2,Breakpoints-method",
        "commonAlignC2<-",
        "commonAlignC2<-,Breakpoints,list-method",
        "commonBpsC1",
        "commonBpsC1,Breakpoints-method",
        "commonBpsC1<-",
        "commonBpsC1<-,Breakpoints,list-method",
        "commonBpsC2",
        "commonBpsC2,Breakpoints-method",
        "commonBpsC2<-",
        "commonBpsC2<-,Breakpoints,list-method",
        "length,Breakpoints-method",
        "mergeBreakpoints,Breakpoints,missing,list-method",
        "mergeBreakpoints,Breakpoints,missing,missing-method",
        "mergeBreakpoints,Breakpoints,numeric,missing-method",
        "names,Breakpoints-method",
        "names<-,Breakpoints,ANY-method",
        "plotChimericReads,Breakpoints-method",
        "seqsC1",
        "seqsC1,Breakpoints-method",
        "seqsC1<-",
        "seqsC1<-,Breakpoints,list-method",
        "seqsC2",
        "seqsC2,Breakpoints-method",
        "seqsC2<-",
        "seqsC2<-,Breakpoints,list-method",
        "[,Breakpoints,ANY,ANY,ANY-method",
        "[,Breakpoints,ANY,ANY-method"
      ]
    },
    {
      "page": "calculateTiTv",
      "title": "Calculate transition transversion ratio",
      "topics": [
        "calculateTiTv",
        "calculateTiTv,AVASet-method",
        "calculateTiTv,MapperSet-method"
      ]
    },
    {
      "page": "captureArray",
      "title": "Custom capture array design",
      "topics": [
        "captureArray"
      ]
    },
    {
      "page": "complexity.dust",
      "title": "Sequence Complexity Using The DUST Algorithm",
      "topics": [
        "complexity.dust",
        "complexity.dust,DNAStringSet-method",
        "complexity.dust,SFFContainer-method",
        "complexity.dust,ShortRead-method"
      ]
    },
    {
      "page": "complexity.entropy",
      "title": "Sequence Complexity Using The Shannon-Wiener Algorithm",
      "topics": [
        "complexity.entropy",
        "complexity.entropy,DNAStringSet-method",
        "complexity.entropy,SFFContainer-method",
        "complexity.entropy,ShortRead-method"
      ]
    },
    {
      "page": "convertCigar",
      "title": "Basic functions for CIGAR strings",
      "topics": [
        "convertCigar",
        "extendedCIGARToList",
        "listToExtendedCIGAR"
      ]
    },
    {
      "page": "coverageOnTarget",
      "title": "Computes the coverage restricted to the target region.",
      "topics": [
        "coverageOnTarget",
        "coverageOnTarget,list,GRanges-method"
      ]
    },
    {
      "page": "demultiplexReads",
      "title": "Performs MID/Multiplex filtering",
      "topics": [
        "demultiplexReads",
        "demultiplexReads,XStringSet,XStringSet,missing,logical-method",
        "demultiplexReads,XStringSet,XStringSet,missing,missing-method",
        "demultiplexReads,XStringSet,XStringSet,numeric,logical-method",
        "demultiplexReads,XStringSet,XStringSet,numeric,missing-method"
      ]
    },
    {
      "page": "detectBreakpoints",
      "title": "Clustering and consensus breakpoint detection for chimeric reads",
      "topics": [
        "detectBreakpoints",
        "detectBreakpoints,list-method"
      ]
    },
    {
      "page": "dinucleotideOddsRatio",
      "title": "Dinucleotide Odds Ratio",
      "topics": [
        "dinucleotideOddsRatio",
        "dinucleotideOddsRatio,DNAStringSet-method",
        "dinucleotideOddsRatio,SFFContainer-method",
        "dinucleotideOddsRatio,ShortRead-method"
      ]
    },
    {
      "page": "fDataAmp",
      "title": "Access the amplicon data of an AVASet.",
      "topics": [
        "fDataAmp"
      ]
    },
    {
      "page": "featureDataAmp",
      "title": "Access the amplicon data of an AVASet",
      "topics": [
        "featureDataAmp"
      ]
    },
    {
      "page": "filterChimericReads",
      "title": "Extract chimeric reads and apply filtering steps to remove artificial chimeric reads.",
      "topics": [
        "filterChimericReads",
        "filterChimericReads,list,IntegerRangesList,DNAString,missing,missing-method",
        "filterChimericReads,list,IntegerRangesList,DNAString,numeric,numeric-method",
        "filterChimericReads,list,IntegerRangesList,missing,missing,missing-method",
        "filterChimericReads,list,IntegerRangesList,missing,numeric,numeric-method",
        "filterChimericReads,list,missing,DNAString,missing,missing-method",
        "filterChimericReads,list,missing,DNAString,numeric,numeric-method",
        "filterChimericReads,list,missing,missing,missing,missing-method",
        "filterChimericReads,list,missing,missing,numeric,numeric-method"
      ]
    },
    {
      "page": "flowgramBarplot",
      "title": "Create A Barplot Of The Flow Intensities",
      "topics": [
        "flowgramBarplot",
        "flowgramBarplot,SFFRead-method"
      ]
    },
    {
      "page": "gcContent",
      "title": "Calculate The Overall GC-Content",
      "topics": [
        "gcContent",
        "gcContent,DNAStringSet-method",
        "gcContent,SFFContainer-method",
        "gcContent,ShortRead-method"
      ]
    },
    {
      "page": "gcContentHist",
      "title": "GC-Content Histogram",
      "topics": [
        "gcContentHist",
        "gcContentHist,DNAStringSet-method",
        "gcContentHist,SFFContainer-method",
        "gcContentHist,ShortRead-method"
      ]
    },
    {
      "page": "gcPerPosition",
      "title": "GC-Content Per Position",
      "topics": [
        "gcPerPosition",
        "gcPerPosition,DNAStringSet-method",
        "gcPerPosition,SFFContainer-method",
        "gcPerPosition,ShortRead-method"
      ]
    },
    {
      "page": "genomeSequencerMIDs",
      "title": "Retrieve GS multiplex sequences",
      "topics": [
        "genomeSequencerMIDs",
        "genomeSequencerMIDs,character-method",
        "genomeSequencerMIDs,missing-method"
      ]
    },
    {
      "page": "getAlignedReads",
      "title": "Import reads from an Amplicon Variant Analyzer project",
      "topics": [
        "getAlignedReads",
        "getAlignedReads,AVASet-method"
      ]
    },
    {
      "page": "getAminoAbbr",
      "title": "Get amino acid abbreviations",
      "topics": [
        "getAminoAbbr"
      ]
    },
    {
      "page": "getVariantPercentages",
      "title": "Variant coverage",
      "topics": [
        "getVariantPercentages"
      ]
    },
    {
      "page": "homopolymerHist",
      "title": "Create A Histogram Of The Homopolymer Stretches",
      "topics": [
        "homopolymerHist",
        "homopolymerHist,SFFRead-method"
      ]
    },
    {
      "page": "htmlReport",
      "title": "HTML-Report Builder for the AVASet and MapperSet",
      "topics": [
        "htmlReport"
      ]
    },
    {
      "page": "MapperSet",
      "title": "Creating a MapperSet",
      "topics": [
        "MapperSet",
        "MapperSet,character-method"
      ]
    },
    {
      "page": "MapperSet-class",
      "title": "Class to Contain GS Reference Mapper Output",
      "topics": [
        "annotateVariants,MapperSet-method",
        "getReadStatus",
        "getReadStatus,MapperSet-method",
        "getVariantPercentages,MapperSet-method",
        "htmlReport,MapperSet-method",
        "MapperSet-class",
        "setVariantFilter,MapperSet-method"
      ]
    },
    {
      "page": "mapperSetExample",
      "title": "GS Reference Mapper data import",
      "topics": [
        "mapperSetExample"
      ]
    },
    {
      "page": "mergeBreakpoints",
      "title": "Identify and merge related breakpoints caused by the same variant.",
      "topics": [
        "mergeBreakpoints"
      ]
    },
    {
      "page": "mutationInfo",
      "title": "Example data for 'plotVariants'",
      "topics": [
        "mutationInfo"
      ]
    },
    {
      "page": "nucleotideCharts",
      "title": "Nucleotide Charts",
      "topics": [
        "nucleotideCharts",
        "nucleotideCharts,DNAStringSet-method",
        "nucleotideCharts,SFFContainer-method",
        "nucleotideCharts,ShortRead-method"
      ]
    },
    {
      "page": "plotAmpliconCoverage",
      "title": "Creates a plot visualizing the number of reads per amplicon",
      "topics": [
        "plotAmpliconCoverage",
        "plotAmpliconCoverage,AVASet,character,logical-method",
        "plotAmpliconCoverage,AVASet,character,missing-method",
        "plotAmpliconCoverage,AVASet,missing,logical-method",
        "plotAmpliconCoverage,AVASet,missing,missing-method"
      ]
    },
    {
      "page": "plotChimericReads",
      "title": "Plots chimeric reads",
      "topics": [
        "plotChimericReads"
      ]
    },
    {
      "page": "plotVariants",
      "title": "Plots variant positions",
      "topics": [
        "plotVariants",
        "plotVariants,AnnotatedVariants,character-method",
        "plotVariants,data.frame,character-method"
      ]
    },
    {
      "page": "plotVariationFrequency",
      "title": "Create an AVA style variation frequency plot",
      "topics": [
        "plotVariationFrequency",
        "plotVariationFrequency,character,numeric-method"
      ]
    },
    {
      "page": "positionQualityBoxplot",
      "title": "Boxplot Of The Quality For Each Position",
      "topics": [
        "positionQualityBoxplot",
        "positionQualityBoxplot,QualityScaledDNAStringSet-method",
        "positionQualityBoxplot,SFFContainer-method",
        "positionQualityBoxplot,ShortReadQ-method"
      ]
    },
    {
      "page": "qualityReportSFF",
      "title": "Function To Create A Quality Report In PDF Format",
      "topics": [
        "qualityReportSFF"
      ]
    },
    {
      "page": "readLengthHist",
      "title": "Histogram Of The Read Lengths",
      "topics": [
        "readLengthHist",
        "readLengthHist,DNAStringSet-method",
        "readLengthHist,SFFContainer-method",
        "readLengthHist,ShortRead-method"
      ]
    },
    {
      "page": "readLengthStats",
      "title": "Statistics For The Read Lengths",
      "topics": [
        "readLengthStats",
        "readLengthStats,DNAStringSet-method",
        "readLengthStats,SFFContainer-method",
        "readLengthStats,ShortRead-method"
      ]
    },
    {
      "page": "readSFF",
      "title": "Function To Read In Roche's .sff Files",
      "topics": [
        "readSFF"
      ]
    },
    {
      "page": "readsOnTarget",
      "title": "Check for each read whether it aligns within the given region.",
      "topics": [
        "readsOnTarget",
        "readsOnTarget,list,GRanges-method"
      ]
    },
    {
      "page": "referenceSequences",
      "title": "Access the reference sequences of an AVASet",
      "topics": [
        "referenceSequences"
      ]
    },
    {
      "page": "regions",
      "title": "Example data for 'plotVariants'",
      "topics": [
        "regions"
      ]
    },
    {
      "page": "removeLinker",
      "title": "Remove linker sequences located at the start of short reads",
      "topics": [
        "removeLinker",
        "removeLinker,XStringSet,DNAString,logical,numeric,numeric-method",
        "removeLinker,XStringSet,DNAString,missing,missing,missing-method"
      ]
    },
    {
      "page": "sequenceCaptureLinkers",
      "title": "Retrieve NimbleGen's sequence capture linkers",
      "topics": [
        "sequenceCaptureLinkers",
        "sequenceCaptureLinkers,character-method",
        "sequenceCaptureLinkers,missing-method"
      ]
    },
    {
      "page": "sequenceQualityHist",
      "title": "A Histogram Of The Sequence Qualities",
      "topics": [
        "sequenceQualityHist",
        "sequenceQualityHist,QualityScaledDNAStringSet-method",
        "sequenceQualityHist,SFFContainer-method",
        "sequenceQualityHist,ShortReadQ-method"
      ]
    },
    {
      "page": "setVariantFilter",
      "title": "Filters output of variant information",
      "topics": [
        "setVariantFilter"
      ]
    },
    {
      "page": "sff2fastq",
      "title": "Write A SFFContainer Object To A FASTQ File",
      "topics": [
        "sff2fastq",
        "sff2fastq,SFFContainer-method"
      ]
    },
    {
      "page": "SFFContainer-class",
      "title": "Class '\"SFFContainer\"'",
      "topics": [
        "addRead",
        "addRead,SFFContainer,SFFRead-method",
        "clipAdapterLeft",
        "clipAdapterLeft,SFFContainer-method",
        "clipAdapterLeft<-",
        "clipAdapterLeft<-,SFFContainer,numeric-method",
        "clipAdapterRight",
        "clipAdapterRight,SFFContainer-method",
        "clipAdapterRight<-",
        "clipAdapterRight<-,SFFContainer,numeric-method",
        "clipQualityLeft",
        "clipQualityLeft,SFFContainer-method",
        "clipQualityLeft<-",
        "clipQualityLeft<-,SFFContainer,numeric-method",
        "clipQualityRight",
        "clipQualityRight,SFFContainer-method",
        "clipQualityRight<-",
        "clipQualityRight<-,SFFContainer,numeric-method",
        "flowChars",
        "flowChars,SFFContainer-method",
        "flowChars<-",
        "flowChars<-,SFFContainer,character-method",
        "flowgramFormat",
        "flowgramFormat,SFFContainer-method",
        "flowgramFormat<-",
        "flowgramFormat<-,SFFContainer,numeric-method",
        "flowgrams",
        "flowgrams,SFFContainer-method",
        "flowgrams<-",
        "flowgrams<-,SFFContainer,list-method",
        "flowIndexes",
        "flowIndexes,SFFContainer-method",
        "flowIndexes<-",
        "flowIndexes<-,SFFContainer,list-method",
        "getRead",
        "getRead,SFFContainer,character-method",
        "keySequence",
        "keySequence,SFFContainer-method",
        "keySequence<-",
        "keySequence<-,SFFContainer,character-method",
        "name",
        "name,SFFContainer-method",
        "name<-",
        "name<-,SFFContainer,character-method",
        "reads",
        "reads,SFFContainer-method",
        "reads<-",
        "reads<-,SFFContainer,QualityScaledDNAStringSet-method",
        "SFFContainer",
        "SFFContainer-class",
        "[,SFFContainer,ANY,ANY,ANY-method",
        "[,SFFContainer,ANY,ANY-method"
      ]
    },
    {
      "page": "SFFRead-class",
      "title": "Class '\"SFFRead\"'",
      "topics": [
        "clipAdapterLeft,SFFRead-method",
        "clipAdapterLeft<-,SFFRead,numeric-method",
        "clipAdapterRight,SFFRead-method",
        "clipAdapterRight<-,SFFRead,numeric-method",
        "clipQualityLeft,SFFRead-method",
        "clipQualityLeft<-,SFFRead,numeric-method",
        "clipQualityRight,SFFRead-method",
        "clipQualityRight<-,SFFRead,numeric-method",
        "flowChars,SFFRead-method",
        "flowChars<-,SFFRead,character-method",
        "flowgram",
        "flowgram,SFFRead-method",
        "flowgram<-",
        "flowgram<-,SFFRead,numeric-method",
        "flowgramFormat,SFFRead-method",
        "flowgramFormat<-,SFFRead,numeric-method",
        "flowIndexes,SFFRead-method",
        "flowIndexes<-,SFFRead,numeric-method",
        "keySequence,SFFRead-method",
        "keySequence<-,SFFRead,character-method",
        "name,SFFRead-method",
        "name<-,SFFRead,character-method",
        "quality,SFFRead-method",
        "quality<-",
        "quality<-,SFFRead,BString-method",
        "read",
        "read,SFFRead-method",
        "read<-",
        "read<-,SFFRead,QualityScaledDNAStringSet-method",
        "SFFRead",
        "SFFRead-class"
      ]
    },
    {
      "page": "variants",
      "title": "Example data for 'plotVariants'",
      "topics": [
        "variants"
      ]
    },
    {
      "page": "writeSFF",
      "title": "Function To Write Files In Roche's .sff Format",
      "topics": [
        "writeSFF"
      ]
    }
  ],
  "_rundeps": [
    "abind",
    "AnnotationDbi",
    "askpass",
    "BH",
    "Biobase",
    "BiocBaseUtils",
    "BiocFileCache",
    "BiocGenerics",
    "BiocIO",
    "BiocParallel",
    "biomaRt",
    "Biostrings",
    "bit",
    "bit64",
    "bitops",
    "blob",
    "BSgenome",
    "cachem",
    "cigarillo",
    "cli",
    "codetools",
    "cpp11",
    "crayon",
    "curl",
    "DBI",
    "dbplyr",
    "DelayedArray",
    "deldir",
    "dplyr",
    "fastmap",
    "filelock",
    "formatR",
    "futile.logger",
    "futile.options",
    "generics",
    "GenomicAlignments",
    "GenomicFeatures",
    "GenomicRanges",
    "glue",
    "hms",
    "httr",
    "httr2",
    "hwriter",
    "interp",
    "IRanges",
    "jpeg",
    "jsonlite",
    "KEGGREST",
    "lambda.r",
    "lattice",
    "latticeExtra",
    "lifecycle",
    "magrittr",
    "MASS",
    "Matrix",
    "MatrixGenerics",
    "matrixStats",
    "memoise",
    "mime",
    "openssl",
    "pillar",
    "pkgconfig",
    "png",
    "prettyunits",
    "progress",
    "purrr",
    "pwalign",
    "R2HTML",
    "R6",
    "rappdirs",
    "RColorBrewer",
    "Rcpp",
    "RcppEigen",
    "RCurl",
    "restfulr",
    "Rhtslib",
    "rjson",
    "rlang",
    "Rsamtools",
    "RSQLite",
    "rtracklayer",
    "S4Arrays",
    "S4Vectors",
    "Seqinfo",
    "ShortRead",
    "snow",
    "SparseArray",
    "stringi",
    "stringr",
    "SummarizedExperiment",
    "sys",
    "TeachingDemos",
    "tibble",
    "tidyr",
    "tidyselect",
    "utf8",
    "VariantAnnotation",
    "vctrs",
    "withr",
    "XML",
    "xml2",
    "xtable",
    "XVector",
    "yaml"
  ],
  "_vignettes": [
    {
      "source": "vignette.Rnw",
      "filename": "vignette.pdf",
      "title": "A package for importing and analyzing data from Roche's Genome Sequencer System",
      "engine": "utils::Sweave",
      "headings": [],
      "created": "2013-10-13 16:28:23",
      "modified": "2026-04-05 16:01:06",
      "commits": 4
    }
  ],
  "_score": 3.4771212547196626,
  "_indexed": true,
  "_nocasepkg": "r453plus1toolbox",
  "_universes": [
    "bioc"
  ],
  "_failure": {
    "version": "1.63.0",
    "commit": {
      "id": "8d8c63f53162b2fabd10980c3ac7e77f71e31e9e",
      "author": "A Wokaty <andres.wokaty@sph.cuny.edu>",
      "committer": "A Wokaty <andres.wokaty@sph.cuny.edu>",
      "message": "bump x.y.z version to odd y following creation of RELEASE_3_23 branch\n",
      "time": 1777379696
    },
    "buildurl": "https://github.com/r-universe/bioc/actions/runs/26658028437",
    "date": "2026-06-01T06:35:20.448Z",
    "job": {
      "job": 78798310844,
      "time": 292,
      "config": "source",
      "r": "4.6.0",
      "check": "ERROR",
      "artifact": "7324828929"
    }
  },
  "_binaries": [
    {
      "r": "4.7.0",
      "os": "linux",
      "version": "1.63.0",
      "date": "2026-04-30T15:38:09.000Z",
      "distro": "noble",
      "arch": "aarch64",
      "commit": "8d8c63f53162b2fabd10980c3ac7e77f71e31e9e",
      "fileid": "1a227243ff50fb4957f7376243e2419601f908cc050e43054ca7f29f96040cdf",
      "status": "failure",
      "check": "ERROR",
      "buildurl": "https://github.com/r-universe/bioc/actions/runs/25173438673"
    },
    {
      "r": "4.7.0",
      "os": "linux",
      "version": "1.63.0",
      "date": "2026-04-30T15:44:27.000Z",
      "distro": "noble",
      "arch": "x86_64",
      "commit": "8d8c63f53162b2fabd10980c3ac7e77f71e31e9e",
      "fileid": "e35dec9287804f7ad6f5ca0c7c100c85a375e52cb5dd0ded72c44215e4a74c69",
      "status": "success",
      "check": "NOTE",
      "buildurl": "https://github.com/r-universe/bioc/actions/runs/25173438673"
    },
    {
      "r": "4.6.0",
      "os": "linux",
      "version": "1.63.0",
      "date": "2026-04-30T15:36:50.000Z",
      "distro": "noble",
      "arch": "aarch64",
      "commit": "8d8c63f53162b2fabd10980c3ac7e77f71e31e9e",
      "fileid": "e7b869d1c28005945cf1cf1d3b1aaec488a7a8802140b0335bfd256427977bd1",
      "status": "success",
      "check": "NOTE",
      "buildurl": "https://github.com/r-universe/bioc/actions/runs/25173438673"
    },
    {
      "r": "4.6.0",
      "os": "linux",
      "version": "1.63.0",
      "date": "2026-04-30T15:43:11.000Z",
      "distro": "noble",
      "arch": "x86_64",
      "commit": "8d8c63f53162b2fabd10980c3ac7e77f71e31e9e",
      "fileid": "cf5584c082d1178d44e7e566886703946b1706c8639408fe19762e6461716a32",
      "status": "success",
      "check": "NOTE",
      "buildurl": "https://github.com/r-universe/bioc/actions/runs/25173438673"
    },
    {
      "r": "4.5.3",
      "os": "mac",
      "version": "1.63.0",
      "date": "2026-04-30T17:14:28.000Z",
      "arch": "aarch64",
      "commit": "8d8c63f53162b2fabd10980c3ac7e77f71e31e9e",
      "fileid": "f194a7082af3effa51b997cfd491f255e0db03f0150cce286c4f8d539c06f23c",
      "status": "success",
      "check": "NOTE",
      "buildurl": "https://github.com/r-universe/bioc/actions/runs/25173438673"
    },
    {
      "r": "4.5.3",
      "os": "mac",
      "version": "1.63.0",
      "date": "2026-04-30T16:20:20.000Z",
      "arch": "x86_64",
      "commit": "8d8c63f53162b2fabd10980c3ac7e77f71e31e9e",
      "fileid": "2c6f55a02a75c8d2d4b5430be8d4569c16386ac86b824c5598c4233fa69fbe99",
      "status": "success",
      "check": "NOTE",
      "buildurl": "https://github.com/r-universe/bioc/actions/runs/25173438673"
    },
    {
      "r": "4.6.0",
      "os": "mac",
      "version": "1.63.0",
      "date": "2026-04-30T17:14:04.000Z",
      "arch": "aarch64",
      "commit": "8d8c63f53162b2fabd10980c3ac7e77f71e31e9e",
      "fileid": "7eda08aac179e8e0e6ce54c4e39ccd4dede7fabda57b465b5d43b2aa30f18aec",
      "status": "success",
      "check": "NOTE",
      "buildurl": "https://github.com/r-universe/bioc/actions/runs/25173438673"
    },
    {
      "r": "4.6.0",
      "os": "mac",
      "version": "1.63.0",
      "date": "2026-04-30T16:21:51.000Z",
      "arch": "x86_64",
      "commit": "8d8c63f53162b2fabd10980c3ac7e77f71e31e9e",
      "fileid": "20cf7c776e0b48556dacc84b8aecfe7a413a2b127751f8a596e2f50352ce6ba9",
      "status": "success",
      "check": "NOTE",
      "buildurl": "https://github.com/r-universe/bioc/actions/runs/25173438673"
    },
    {
      "r": "4.5.1",
      "os": "wasm",
      "version": "1.63.0",
      "date": "2026-04-30T15:49:56.000Z",
      "arch": "emscripten",
      "commit": "8d8c63f53162b2fabd10980c3ac7e77f71e31e9e",
      "fileid": "8d70a868f59b50d2c76ed7440a2041590e40808c92d03f1c877a4aa3d81c178b",
      "status": "success",
      "buildurl": "https://github.com/r-universe/bioc/actions/runs/25173438673"
    },
    {
      "r": "4.7.0",
      "os": "win",
      "version": "1.63.0",
      "date": "2026-04-30T15:40:55.000Z",
      "arch": "x86_64",
      "commit": "8d8c63f53162b2fabd10980c3ac7e77f71e31e9e",
      "fileid": "5fab55dd0b8864ae468506f2cd8d9d033a6894268702620e7fd619156ba901e6",
      "status": "success",
      "check": "NOTE",
      "buildurl": "https://github.com/r-universe/bioc/actions/runs/25173438673"
    },
    {
      "r": "4.5.3",
      "os": "win",
      "version": "1.63.0",
      "date": "2026-04-30T15:41:56.000Z",
      "arch": "x86_64",
      "commit": "8d8c63f53162b2fabd10980c3ac7e77f71e31e9e",
      "fileid": "dae041439aee59344217cddff85c37eeb2fe511d2fbf2bdff8363d1869669c4c",
      "status": "success",
      "check": "NOTE",
      "buildurl": "https://github.com/r-universe/bioc/actions/runs/25173438673"
    },
    {
      "r": "4.6.0",
      "os": "win",
      "version": "1.63.0",
      "date": "2026-04-30T15:40:41.000Z",
      "arch": "x86_64",
      "commit": "8d8c63f53162b2fabd10980c3ac7e77f71e31e9e",
      "fileid": "fbb118420b347b385f942123d4f1412148d75168e44eda18f36577bf877afc40",
      "status": "success",
      "check": "NOTE",
      "buildurl": "https://github.com/r-universe/bioc/actions/runs/25173438673"
    }
  ]
}