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      "object": "SPSs",
      "file": "SPSs.rda",
      "class": [
        "character"
      ],
      "fields": [],
      "table": false,
      "tojson": true
    }
  ],
  "_help": [
    {
      "page": "createFrequencyMat",
      "title": "Create frequency matrix",
      "topics": [
        "createFrequencyMat"
      ]
    },
    {
      "page": "frequencyScoring",
      "title": "Frequency scoring",
      "topics": [
        "frequencyScoring"
      ]
    },
    {
      "page": "getSPS",
      "title": "Generate set of stable phosphoporylated sites",
      "topics": [
        "getSPS"
      ]
    },
    {
      "page": "hSEGs",
      "title": "A list of Stably Expressed Genes (SEGs)",
      "topics": [
        "hSEGs"
      ]
    },
    {
      "page": "KinaseFamily",
      "title": "KinaseFamily",
      "topics": [
        "KinaseFamily"
      ]
    },
    {
      "page": "kinaseSubstrateHeatmap",
      "title": "Kinase-substrate annotation prioritisation heatmap",
      "topics": [
        "kinaseSubstrateHeatmap"
      ]
    },
    {
      "page": "kinaseSubstratePred",
      "title": "kinaseSubstratePred",
      "topics": [
        "kinaseSubstratePred"
      ]
    },
    {
      "page": "kinaseSubstrateProfile",
      "title": "Kinase substrate profiling",
      "topics": [
        "kinaseSubstrateProfile"
      ]
    },
    {
      "page": "kinaseSubstrateScore",
      "title": "Kinase substrate scoring",
      "topics": [
        "kinaseSubstrateScore"
      ]
    },
    {
      "page": "matANOVA",
      "title": "ANOVA test",
      "topics": [
        "matANOVA"
      ]
    },
    {
      "page": "meanAbundance",
      "title": "Obtain average expression from replicates",
      "topics": [
        "meanAbundance"
      ]
    },
    {
      "page": "medianScaling",
      "title": "Median centering and scaling",
      "topics": [
        "medianScaling"
      ]
    },
    {
      "page": "minmax",
      "title": "Minmax scaling",
      "topics": [
        "minmax"
      ]
    },
    {
      "page": "mIntersect",
      "title": "Multi-intersection, union",
      "topics": [
        "mIntersect",
        "mUnion"
      ]
    },
    {
      "page": "motif.human.list",
      "title": "List of human kinase motifs",
      "topics": [
        "motif.human.list"
      ]
    },
    {
      "page": "motif.mouse.list",
      "title": "List of mouse kinase motifs",
      "topics": [
        "motif.mouse.list"
      ]
    },
    {
      "page": "motif.rat.list",
      "title": "List of rat kinase motifs",
      "topics": [
        "motif.rat.list"
      ]
    },
    {
      "page": "mSEGs",
      "title": "A list of Stably Expressed Genes (SEGs)",
      "topics": [
        "mSEGs"
      ]
    },
    {
      "page": "pathwayOverrepresent",
      "title": "phosphosite/Gene set over-representation analysis",
      "topics": [
        "pathwayOverrepresent"
      ]
    },
    {
      "page": "pathwayRankBasedEnrichment",
      "title": "Phosphosite/Gene set enrichment analysis",
      "topics": [
        "pathwayRankBasedEnrichment"
      ]
    },
    {
      "page": "phosCollapse",
      "title": "Summarising phosphosites to proteins",
      "topics": [
        "phosCollapse"
      ]
    },
    {
      "page": "phospho.cells.Ins",
      "title": "phospho.cells.Ins",
      "topics": [
        "phospho.cells.Ins",
        "phospho.cells.Ins.pe"
      ]
    },
    {
      "page": "phospho.L6.ratio",
      "title": "phospho.L6.ratio",
      "topics": [
        "phospho.L6.ratio"
      ]
    },
    {
      "page": "phospho.L6.ratio.pe",
      "title": "phospho_L6_ratio_pe",
      "topics": [
        "phospho.L6.ratio.pe"
      ]
    },
    {
      "page": "phospho.liver.Ins.TC.ratio.RUV",
      "title": "phospho_liverInsTC_RUV_sample",
      "topics": [
        "phospho.liver.Ins.TC.ratio.RUV"
      ]
    },
    {
      "page": "phospho.liver.Ins.TC.ratio.RUV.pe",
      "title": "phospho.liver.Ins.TC.ratio.RUV.pe",
      "topics": [
        "phospho.liver.Ins.TC.ratio.RUV.pe"
      ]
    },
    {
      "page": "PhosphoExperiment",
      "title": "The PhosphoExperiment class",
      "topics": [
        "coerce,",
        "PhosphoExperiment",
        "PhosphoExperiment-class",
        "PhosphoExperiment-method",
        "SummarizedExperiment,"
      ]
    },
    {
      "page": "PhosphoSite.human",
      "title": "PhosphoSitePlus annotations for human",
      "topics": [
        "PhosphoSite.human"
      ]
    },
    {
      "page": "PhosphoSite.mouse",
      "title": "PhosphoSitePlus annotations for mouse",
      "topics": [
        "PhosphoSite.mouse"
      ]
    },
    {
      "page": "PhosphoSite.rat",
      "title": "PhosphoSitePlus annotations for rat",
      "topics": [
        "PhosphoSite.rat"
      ]
    },
    {
      "page": "plotKinaseNetwork",
      "title": "Plot kinase network",
      "topics": [
        "plotKinaseNetwork"
      ]
    },
    {
      "page": "plotQC",
      "title": "A set of function for data QC plot",
      "topics": [
        "plotQC"
      ]
    },
    {
      "page": "plotSignalomeMap",
      "title": "Plot signalome map",
      "topics": [
        "plotSignalomeMap"
      ]
    },
    {
      "page": "PhosphoExperiment-methods",
      "title": "PhosphoExperiment object accessors",
      "topics": [
        "GeneSymbol",
        "GeneSymbol,PhosphoExperiment-method",
        "GeneSymbol<-",
        "GeneSymbol<-,PhosphoExperiment-method",
        "Localisation",
        "Localisation,PhosphoExperiment-method",
        "Localisation<-",
        "Localisation<-,PhosphoExperiment-method",
        "PPE-accessors",
        "Residue",
        "Residue,PhosphoExperiment-method",
        "Residue<-",
        "Residue<-,PhosphoExperiment-method",
        "Sequence",
        "Sequence,PhosphoExperiment-method",
        "Sequence<-",
        "Sequence<-,PhosphoExperiment-method",
        "Site",
        "Site,PhosphoExperiment-method",
        "Site<-",
        "Site<-,PhosphoExperiment-method",
        "UniprotID",
        "UniprotID,PhosphoExperiment-method",
        "UniprotID<-",
        "UniprotID<-,PhosphoExperiment-method"
      ]
    },
    {
      "page": "PhosphoExperiment-operate",
      "title": "PhosphoExperiment object subset, combine methods",
      "topics": [
        "cbind",
        "cbind,PhosphoExperiment-method",
        "PPE-operate",
        "rbind",
        "rbind,PhosphoExperiment-method",
        "[",
        "[,PhosphoExperiment,ANY,ANY,ANY-method",
        "[<-",
        "[<-,PhosphoExperiment,ANY,ANY,ANY-method"
      ]
    },
    {
      "page": "ptImpute",
      "title": "Paired-tail (pt) based impute",
      "topics": [
        "ptImpute"
      ]
    },
    {
      "page": "RUVphospho",
      "title": "RUV for phosphoproteomics data normalisation",
      "topics": [
        "RUVphospho",
        "RUVproteome"
      ]
    },
    {
      "page": "scImpute",
      "title": "Site- and condition-specific (sc) impute",
      "topics": [
        "scImpute"
      ]
    },
    {
      "page": "selectGrps",
      "title": "Select by treatment groups (replicate block)",
      "topics": [
        "selectGrps"
      ]
    },
    {
      "page": "selectLocalisedSites",
      "title": "Select phosphosites by localisation score",
      "topics": [
        "selectLocalisedSites"
      ]
    },
    {
      "page": "selectOverallPercent",
      "title": "Select phosphosite by percentage of quantification",
      "topics": [
        "selectOverallPercent"
      ]
    },
    {
      "page": "selectTimes",
      "title": "selectTimes",
      "topics": [
        "selectTimes"
      ]
    },
    {
      "page": "Signalomes",
      "title": "PhosR Signalomes",
      "topics": [
        "Signalomes"
      ]
    },
    {
      "page": "siteAnnotate",
      "title": "Phosphosite annotation",
      "topics": [
        "siteAnnotate"
      ]
    },
    {
      "page": "SPSs",
      "title": "A list of Stably Phosphorylated Sites (SPSs)",
      "topics": [
        "SPSs"
      ]
    },
    {
      "page": "standardise",
      "title": "Standardisation",
      "topics": [
        "standardise"
      ]
    },
    {
      "page": "tImpute",
      "title": "Tail-based impute",
      "topics": [
        "tImpute"
      ]
    }
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      "source": "PhosR.Rmd",
      "filename": "PhosR.html",
      "title": "An introduction to PhosR package",
      "author": "Taiyun Kim, Hani Jieun Kim, Di Xiao, Pengyi Yang",
      "engine": "knitr::rmarkdown",
      "headings": [
        "Introduction",
        "Installation",
        "Loading packages and data",
        "Setting up the PhosphoExperiment object",
        "Part A. Preprocessing",
        "Imputation",
        "Setting up the data",
        "Filtering of phosphosites",
        "Imputation of phosphosites",
        "Site- and condition-specific imputation",
        "Paired tail-based imputation",
        "Quantification plots",
        "Batch correction",
        "Diagnosing batch effect",
        "Correcting batch effect",
        "Quality control",
        "Generating SPSs",
        "Part B. Downstream analysis",
        "Pathway analysis",
        "1-dimensional enrichment analysis",
        "Prepare the Reactome annotation",
        "Perform 1D gene-centric pathway analysis",
        "2- and 3-dimensional signalling pathway analysis",
        "Site- and gene- centric analysis",
        "Gene-centric analyses of the liver phosphoproteome data",
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        "Signalomes",
        "Generation of kinase-substrate relationship scores",
        "Signalome construction",
        "Generate signalome map",
        "Generate signalome network",
        "Session Info"
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      "created": "2020-06-30 00:48:25",
      "modified": "2021-04-23 12:47:59",
      "commits": 19
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