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  "Package": "MutSeqR",
  "Title": "Analysis of Error-Corrected Sequencing Data for Mutation\nDetection",
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  "Date": "2025-12-09",
  "Authors@R": "c(\nperson(\"Annette E.\", \"Dodge\",\nemail = \"annette.dodge@hc-sc.gc.ca\",\nrole = \"aut\",\ncomment = c(ORCID = \"0000-0002-0446-9055\")),\nperson(\"Andrew\", \"Williams\", role = \"aut\",\ncomment = c(ORCID = \"0000-0002-7637-7686\")),\nperson(\"Danielle P.M.\", \"LeBlanc\", role = \"aut\",\ncomment = c(ORCID = \"0000-0002-3847-8371\")),\nperson(\"David M.\", \"Schuster\", role = \"aut\",\ncomment = c(ORCID = \"0009-0001-6316-4358\")),\nperson(\"Elena\", \"Esina\", role = \"aut\",\ncomment = c(ORCID = \"0009-0002-3443-378X\")),\nperson(\"Clint C.\", \"Valentine\", role = \"aut\",\ncomment = c(ORCID = \"0000-0001-5630-7368\")),\nperson(\"Jesse J.\", \"Salk\", role = \"aut\",\ncomment = c(ORCID = \"0000-0002-7804-0550\")),\nperson(\"Alexander Y.\", \"Maslov\", role = \"aut\"),\nperson(\"Christopher\", \"Bradley\", role = \"aut\"),\nperson(\"Carole L.\", \"Yauk\", role = \"aut\",\ncomment = c(ORCID = \"0000-0002-6725-3454\")),\nperson(\"Francesco\", \"Marchetti\", role = \"aut\",\ncomment = c(ORCID = \"0000-0002-9435-4867\")),\nperson(\"Matthew J.\", \"Meier\",\nemail = \"matthew.meier@hc-sc.gc.ca\",\nrole = c(\"aut\", \"cre\"),\ncomment = c(ORCID = \"0000-0001-8199-8754\")),\nperson(\"Geronimo\", \"Matteo\", role = \"ctb\",\ncomment = c(ORCID = \"0000-0003-0819-4471\")),\nperson(\"Health Canada's Genomics Research and Development Initiative\", role = \"fnd\"),\nperson(\"Canada Research Chairs Program\", role = \"fnd\", comment = \"CRC-2020-00060\"),\nperson(\"Burroughs Wellcome Fund\", role = \"fnd\")\n)",
  "Description": "Standard methods for analysis of mutation data following\nerror- corrected sequencing (ECS) for the purpose of\nmutagencity assessment. Functions include importing the\nmutation lists provided by a variant caller, and a set of\nanalytical tools for statistical testing and visualization of\nmutation data; comparison to COSMIC and/or germline signatures;\netc.",
  "License": "MIT + file LICENSE",
  "biocViews": "Sequencing, SomaticMutation, Visualization,\nGenomicVariation, DriverMutation, StatisticalMethod, GeneTarget",
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  "Repository": "https://bioc.r-universe.dev",
  "Date/Publication": "2026-04-28 13:06:12 UTC",
  "RemoteUrl": "https://github.com/bioc/MutSeqR",
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  "Packaged": {
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    "User": "root"
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  "Author": "Annette E. Dodge [aut] (ORCID: <https://orcid.org/0000-0002-0446-9055>),\nAndrew Williams [aut] (ORCID: <https://orcid.org/0000-0002-7637-7686>),\nDanielle P.M. LeBlanc [aut] (ORCID:\n<https://orcid.org/0000-0002-3847-8371>),\nDavid M. Schuster [aut] (ORCID:\n<https://orcid.org/0009-0001-6316-4358>),\nElena Esina [aut] (ORCID: <https://orcid.org/0009-0002-3443-378X>),\nClint C. Valentine [aut] (ORCID:\n<https://orcid.org/0000-0001-5630-7368>),\nJesse J. Salk [aut] (ORCID: <https://orcid.org/0000-0002-7804-0550>),\nAlexander Y. Maslov [aut],\nChristopher Bradley [aut],\nCarole L. Yauk [aut] (ORCID: <https://orcid.org/0000-0002-6725-3454>),\nFrancesco Marchetti [aut] (ORCID:\n<https://orcid.org/0000-0002-9435-4867>),\nMatthew J. Meier [aut, cre] (ORCID:\n<https://orcid.org/0000-0001-8199-8754>),\nGeronimo Matteo [ctb] (ORCID: <https://orcid.org/0000-0003-0819-4471>),\nHealth Canada's Genomics Research and Development Initiative [fnd],\nCanada Research Chairs Program [fnd] (CRC-2020-00060),\nBurroughs Wellcome Fund [fnd]",
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      "page": "annotate_cpg_sites",
      "title": "Annotate CpG sites",
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      "title": "BMD modeling using PROAST",
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      "title": "BS genome organism dictionary",
      "topics": [
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      "title": "Calculate mutation frequency",
      "topics": [
        "calculate_mf"
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    },
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      "title": "Characterize Variants",
      "topics": [
        "characterize_variants"
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    },
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      "page": "check_required_columns",
      "title": "Check that all required columns are present before proceeding with the function",
      "topics": [
        "check_required_columns"
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      "title": "classify_variation",
      "topics": [
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      "title": "Cleveland Plot",
      "topics": [
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      "title": "A list of reference contexts at different resolutions",
      "topics": [
        "context_list"
      ]
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      "title": "Values used for denominators in frequency calculations",
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      "title": "Filter your mutation data",
      "topics": [
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      ]
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      "title": "Find the appropriate BS genome for the specified organism and genome.",
      "topics": [
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      "title": "Add binomial confidence intervals to mutation frequencies.",
      "topics": [
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      "topics": [
        "get_cpg_mutations"
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    {
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      "title": "Get the coordinates of the CpG sites within your genomic regions",
      "topics": [
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      "title": "A utility function that will return the reference context of a mutation",
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      "title": "Get sequence of genomic target regions",
      "topics": [
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      "title": "Derive end coordinates from a VCF object",
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      "title": "Import tabular mutation data",
      "topics": [
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      "title": "Join Regions Metadata",
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    },
    {
      "page": "import_sample_data",
      "title": "Import Sample Metadata",
      "topics": [
        "import_sample_data"
      ]
    },
    {
      "page": "import_vcf_data",
      "title": "Import a VCF file",
      "topics": [
        "import_vcf_data"
      ]
    },
    {
      "page": "load_regions_file",
      "title": "Imports the regions file",
      "topics": [
        "load_regions_file"
      ]
    },
    {
      "page": "model_mf",
      "title": "Perform linear modelling on mutation frequency for given fixed and random effects",
      "topics": [
        "model_mf"
      ]
    },
    {
      "page": "op",
      "title": "Dictionary of column name synonyms for required mutation data columns",
      "topics": [
        "op"
      ]
    },
    {
      "page": "plot_bubbles",
      "title": "Generate Bubble Plots",
      "topics": [
        "plot_bubbles"
      ]
    },
    {
      "page": "plot_ci",
      "title": "plot_ci",
      "topics": [
        "plot_ci"
      ]
    },
    {
      "page": "plot_lollipop",
      "title": "Plot recurrent mutations in a lollipop plot using ggplot2",
      "topics": [
        "plot_lollipop"
      ]
    },
    {
      "page": "plot_mean_mf",
      "title": "Plot the Mean Mutatation Frequency",
      "topics": [
        "plot_mean_mf"
      ]
    },
    {
      "page": "plot_mf",
      "title": "Plot the Mutation Frequency",
      "topics": [
        "plot_mf"
      ]
    },
    {
      "page": "plot_model_mf",
      "title": "Plot your mf model",
      "topics": [
        "plot_model_mf"
      ]
    },
    {
      "page": "plot_radar",
      "title": "Create a radar plot",
      "topics": [
        "plot_radar"
      ]
    },
    {
      "page": "plot_spectra",
      "title": "Plot spectra",
      "topics": [
        "plot_spectra"
      ]
    },
    {
      "page": "plot_trinucleotide",
      "title": "Plot the trinucleotide spectrum",
      "topics": [
        "plot_trinucleotide"
      ]
    },
    {
      "page": "plot_trinucleotide_heatmap",
      "title": "Create a heatmap plot of mutation subtype proportions.",
      "topics": [
        "plot_trinucleotide_heatmap"
      ]
    },
    {
      "page": "populate_sequence_context",
      "title": "Populate Sequence context",
      "topics": [
        "populate_sequence_context"
      ]
    },
    {
      "page": "print_ascii_art",
      "title": "This function prints ASCII art when the package is loaded",
      "topics": [
        "print_ascii_art"
      ]
    },
    {
      "page": "rdm_fast",
      "title": "rdm_fast",
      "topics": [
        "rdm_fast"
      ]
    },
    {
      "page": "rename_columns",
      "title": "Map column names of mutation data to default column names. A utility function that renames columns of mutation data to default columns names.",
      "topics": [
        "rename_columns"
      ]
    },
    {
      "page": "render_report",
      "title": "Read configuration file and render R Markdown document",
      "topics": [
        "render_report"
      ]
    },
    {
      "page": "reverseComplement",
      "title": "Get the reverse complement of a DNA or RNA sequence.",
      "topics": [
        "reverseComplement"
      ]
    },
    {
      "page": "run_penalized_comparison",
      "title": "run_penalized_comparison",
      "topics": [
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      ]
    },
    {
      "page": "select_optimal_lambda",
      "title": "select_optimal_lambda",
      "topics": [
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    },
    {
      "page": "setup_mutseqr_python",
      "title": "Set up Python environment for MutSeqR",
      "topics": [
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      ]
    },
    {
      "page": "sidak",
      "title": "Correct p-values for multiple comparisons",
      "topics": [
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    },
    {
      "page": "signature_fitting",
      "title": "Run COSMIC signatures comparison using SigProfilerAssignment",
      "topics": [
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      ]
    },
    {
      "page": "spectra_comparison",
      "title": "Compare the overall mutation spectra between groups",
      "topics": [
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    },
    {
      "page": "spectra_comparison_rpdmm",
      "title": "spectra_comparison_rpdmm",
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    {
      "page": "subtype_dict",
      "title": "Subtype Resolution Dictionary",
      "topics": [
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    },
    {
      "page": "subtype_list",
      "title": "A comprehensive list of mutation subtypes at different resolutions",
      "topics": [
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    },
    {
      "page": "vcf_sample_fix",
      "title": "Retrieve the sample column from VCF files",
      "topics": [
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    },
    {
      "page": "write_excel",
      "title": "Write results to Excel tables",
      "topics": [
        "write_excel"
      ]
    },
    {
      "page": "write_mutation_calling_file",
      "title": "Write the mutation calling file to input into the SigProfiler Assignment web application.",
      "topics": [
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    },
    {
      "page": "write_mutational_matrix",
      "title": "Write a Mutational Matrix to input into the sigprofiler web application",
      "topics": [
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    },
    {
      "page": "write_reference_fasta",
      "title": "Write FASTA file of reference sequences.",
      "topics": [
        "write_reference_fasta"
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    },
    {
      "page": "write_vcf_from_mut",
      "title": "Write mutation_data to a VCF file",
      "topics": [
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      "source": "MutSeqR_introduction.Rmd",
      "filename": "MutSeqR_introduction.html",
      "title": "MutSeqR: Error-Corrected Sequencing (ECS) Analysis For Mutagenicity Assessment",
      "author": "Annette E. Dodge, Matthew J. Meier",
      "engine": "knitr::rmarkdown",
      "headings": [
        "Introduction",
        "What is ECS?",
        "Installation",
        "Data import",
        "General Usage",
        "Examples",
        "VCF",
        "Tabular",
        "Region Metadata",
        "Custom Column Names",
        "Output",
        "Variant Filtering",
        "Germline Variants",
        "Quality Assurance",
        "Custom Filtering",
        "Filtering by Regions",
        "Example",
        "Calculating MF",
        "Mutation Counting",
        "Grouping Mutations",
        "Mutation Subtypes",
        "Selecting Variation Types",
        "Correcting Depth",
        "Precalculated Depth",
        "Summary Table",
        "MF Plots",
        "plot_mf",
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        "Exporting Results",
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        "References",
        "Appendix",
        "Session Info"
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      "created": "2025-06-26 12:52:49",
      "modified": "2026-02-06 22:21:44",
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