{ "_id": "6a1ae0b91d7bb097a09ed309", "Package": "CRISPRseek", "Version": "1.53.0", "Title": "Design of guide RNAs in CRISPR genome-editing systems", "Author": "Lihua Julie Zhu Paul Scemama Benjamin R. Holmes Hervé Pagès Kai\nHu Hui Mao Michael Lawrence Isana Veksler-Lublinsky Victor\nAmbros Neil Aronin Michael Brodsky Devin M Burris", "Maintainer": "Lihua Julie Zhu Kai Hu\n", "Date": "2024-11-14", "Description": "The package encompasses functions to find potential guide\nRNAs for the CRISPR-based genome-editing systems including the\nBase Editors and the Prime Editors when supplied with target\nsequences as input. Users have the flexibility to filter\nresulting guide RNAs based on parameters such as the absence of\nrestriction enzyme cut sites or the lack of paired guide RNAs.\nThe package also facilitates genome-wide exploration for\noff-targets, offering features to score and rank off-targets,\nretrieve flanking sequences, and indicate whether the hits are\nlocated within exon regions. All detected guide RNAs are\nannotated with the cumulative scores of the top5 and topN\noff-targets together with the detailed information such as\nmismatch sites and restrictuion enzyme cut sites. The package\nalso outputs INDELs and their frequencies for Cas9 targeted\nsites.", "License": "file LICENSE", "biocViews": "ImmunoOncology, GeneRegulation, SequenceMatching, CRISPR", "Encoding": "UTF-8", "RoxygenNote": "7.3.2", "NeedsCompilation": "no", "VignetteBuilder": "knitr", "Config/pak/sysreqs": "make libbz2-dev libicu-dev liblzma-dev libpng-dev\nlibxml2-dev libssl-dev python3 libx11-dev xz-utils zlib1g-dev", "Repository": "https://bioc.r-universe.dev", "Date/Publication": "2026-04-28 12:38:26 UTC", "RemoteUrl": "https://github.com/bioc/CRISPRseek", "RemoteRef": "HEAD", "RemoteSha": "8b07239a2c84a56133dd75684046b6703894ce9e", "Packaged": { "Date": "2026-05-30 09:02:57 UTC", "User": "root" }, "MD5sum": "a0035e3e21513c0926b6cf6e8139dbe5", "_user": "bioc", "_type": "src", "_file": "CRISPRseek_1.53.0.tar.gz", "_fileid": "f3147c3e7e3c1c0c48d0d0889863274ac61706bc8b3980d418ffacf21f7a612b", "_filesize": 12371275, "_sha256": 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