{
  "_id": "6a168de9acfb0bcc41d779a6",
  "Package": "BUSpaRse",
  "Type": "Package",
  "Title": "kallisto | bustools R utilities",
  "Version": "1.27.0",
  "Date": "2025-09-15",
  "Authors@R": "c(person(\"Lambda\", \"Moses\", email = \"dl3764@columbia.edu\",\nrole = c(\"aut\", \"cre\"),\ncomment = c(ORCID = \"0000-0002-7092-9427\")),\nperson(\"Lior\", \"Pachter\", email = \"lpachter@caltech.edu\",\nrole = c(\"aut\", \"ths\"),\ncomment = c(ORCID = \"0000-0002-9164-6231\")))",
  "Description": "The kallisto | bustools pipeline is a fast and modular set\nof tools to convert single cell RNA-seq reads in fastq files\ninto gene count or transcript compatibility counts (TCC)\nmatrices for downstream analysis. Central to this pipeline is\nthe barcode, UMI, and set (BUS) file format. This package\nserves the following purposes: First, this package allows users\nto manipulate BUS format files as data frames in R and then\nconvert them into gene count or TCC matrices. Furthermore,\nsince R and Rcpp code is easier to handle than pure C++ code,\nusers are encouraged to tweak the source code of this package\nto experiment with new uses of BUS format and different ways to\nconvert the BUS file into gene count matrix. Second, this\npackage can conveniently generate files required to generate\ngene count matrices for spliced and unspliced transcripts for\nRNA velocity. Here biotypes can be filtered and scaffolds and\nhaplotypes can be removed, and the filtered transcriptome can\nbe extracted and written to disk. Third, this package\nimplements utility functions to get transcripts and associated\ngenes required to convert BUS files to gene count matrices, to\nwrite the transcript to gene information in the format required\nby bustools, and to read output of bustools into R as sparses\nmatrices.",
  "BugReports": "https://github.com/BUStools/BUSpaRse/issues",
  "URL": "https://github.com/BUStools/BUSpaRse",
  "RoxygenNote": "7.3.3",
  "Roxygen": "list(markdown = TRUE)",
  "VignetteBuilder": "knitr",
  "LazyData": "TRUE",
  "Collate": "'RcppExports.R' 'annots_from_fa.R' 'biotypes.R' 'get_tx.R'\n'knee_plot.R' 'sparse_matrix.R' 'tr2g.R' 'utils.R' 'velocity.R'\n'velocity_methods.R'",
  "Encoding": "UTF-8",
  "License": "BSD_2_clause + file LICENSE",
  "biocViews": "SingleCell, RNASeq, WorkflowStep",
  "SystemRequirements": "GNU make",
  "Config/pak/sysreqs": "make libbz2-dev libicu-dev liblzma-dev libpng-dev\nlibxml2-dev libssl-dev xz-utils zlib1g-dev",
  "Repository": "https://bioc.r-universe.dev",
  "Date/Publication": "2026-04-28 12:50:47 UTC",
  "RemoteUrl": "https://github.com/bioc/BUSpaRse",
  "RemoteRef": "HEAD",
  "RemoteSha": "b35dd0b3fd69c36057496007d6ba89a9acb35dc2",
  "NeedsCompilation": "yes",
  "Packaged": {
    "Date": "2026-05-25 06:18:25 UTC",
    "User": "root"
  },
  "Author": "Lambda Moses [aut, cre] (ORCID:\n<https://orcid.org/0000-0002-7092-9427>),\nLior Pachter [aut, ths] (ORCID:\n<https://orcid.org/0000-0002-9164-6231>)",
  "Maintainer": "Lambda Moses <dl3764@columbia.edu>",
  "MD5sum": "9c07d18530d823b9a5225e7dc54c2f31",
  "_user": "bioc",
  "_type": "src",
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  "_created": "2026-05-25T06:18:25.000Z",
  "_published": "2026-05-27T06:23:37.864Z",
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  "_assets": [
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  "_homeurl": "https://github.com/bustools/busparse",
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  "_exports": [
    "annots_from_fa_df",
    "annots_from_fa_GRanges",
    "dl_transcriptome",
    "EC2gene",
    "get_inflection",
    "get_knee_df",
    "get_velocity_files",
    "knee_plot",
    "make_sparse_matrix",
    "read_count_output",
    "read_velocity_output",
    "save_tr2g_bustools",
    "sort_tr2g",
    "species2dataset",
    "subset_annot",
    "tr2g_EnsDb",
    "tr2g_ensembl",
    "tr2g_fasta",
    "tr2g_gff3",
    "tr2g_gtf",
    "tr2g_TxDb",
    "transcript2gene"
  ],
  "_datasets": [
    {
      "name": "cellranger_biotypes",
      "title": "Cell Ranger gene biotypes",
      "object": "cellranger_biotypes",
      "class": [
        "character"
      ],
      "fields": [],
      "table": false,
      "tojson": true
    },
    {
      "name": "ensembl_gene_biotypes",
      "title": "Gene biotypes from Ensembl",
      "object": "ensembl_gene_biotypes",
      "class": [
        "character"
      ],
      "fields": [],
      "table": false,
      "tojson": true
    },
    {
      "name": "ensembl_gff_mcols",
      "title": "These are the column names of the 'mcols' when the Ensembl GTF file is read into R as a 'GRanges', including 'gene_id', 'transcript_id', 'biotype', 'description', and so on, and the mandatory tags like 'ID', 'Name', and 'Parent'.",
      "object": "ensembl_gff_mcols",
      "class": [
        "character"
      ],
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      "table": false,
      "tojson": true
    },
    {
      "name": "ensembl_gtf_mcols",
      "title": "Tags in the attributes field of Ensembl GTF files",
      "object": "ensembl_gtf_mcols",
      "class": [
        "character"
      ],
      "fields": [],
      "table": false,
      "tojson": true
    },
    {
      "name": "ensembl_tx_biotypes",
      "title": "Transcript biotypes from Ensembl",
      "object": "ensembl_tx_biotypes",
      "class": [
        "character"
      ],
      "fields": [],
      "table": false,
      "tojson": true
    },
    {
      "name": "refseq_gff_mcols",
      "title": "Tags in the attributes field of RefSeq GFF files",
      "object": "refseq_gff_mcols",
      "class": [
        "character"
      ],
      "fields": [],
      "table": false,
      "tojson": true
    }
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  "_help": [
    {
      "page": "dot-get_velocity_files",
      "title": "Generate RNA velocity files for GRanges",
      "topics": [
        ".get_velocity_files"
      ]
    },
    {
      "page": "annot_circular",
      "title": "Transfer information about circular chromosomes between genome and annotation",
      "topics": [
        "annot_circular"
      ]
    },
    {
      "page": "annots_from_fa_df",
      "title": "Get genome annotation from Ensembl FASTA file",
      "topics": [
        "annots_from_fa_df",
        "annots_from_fa_GRanges"
      ]
    },
    {
      "page": "cellranger_biotypes",
      "title": "Cell Ranger gene biotypes",
      "topics": [
        "cellranger_biotypes"
      ]
    },
    {
      "page": "check_char1",
      "title": "Check that an object is a character vector of length 1",
      "topics": [
        "check_char1"
      ]
    },
    {
      "page": "check_genome",
      "title": "Check for chromosomes in genome but not annotation",
      "topics": [
        "check_genome"
      ]
    },
    {
      "page": "check_gff",
      "title": "Check inputs to tr2g_gtf and tr2g_gff3",
      "topics": [
        "check_gff"
      ]
    },
    {
      "page": "check_tag_present",
      "title": "Check that a tag is present in attribute field of GTF/GFF",
      "topics": [
        "check_tag_present"
      ]
    },
    {
      "page": "check_tx",
      "title": "Check if transcript ID in transcriptome and annotation match",
      "topics": [
        "check_tx"
      ]
    },
    {
      "page": "dl_transcriptome",
      "title": "Download transcriptome from Ensembl",
      "topics": [
        "dl_transcriptome"
      ]
    },
    {
      "page": "EC2gene",
      "title": "Map EC Index to Genes Compatible with the EC",
      "topics": [
        "EC2gene"
      ]
    },
    {
      "page": "ensembl_gene_biotypes",
      "title": "Gene biotypes from Ensembl",
      "topics": [
        "ensembl_gene_biotypes"
      ]
    },
    {
      "page": "ensembl_gff_mcols",
      "title": "These are the column names of the 'mcols' when the Ensembl GTF file is read into R as a 'GRanges', including 'gene_id', 'transcript_id', 'biotype', 'description', and so on, and the mandatory tags like 'ID', 'Name', and 'Parent'.",
      "topics": [
        "ensembl_gff_mcols"
      ]
    },
    {
      "page": "ensembl_gtf_mcols",
      "title": "Tags in the attributes field of Ensembl GTF files",
      "topics": [
        "ensembl_gtf_mcols"
      ]
    },
    {
      "page": "ensembl_tx_biotypes",
      "title": "Transcript biotypes from Ensembl",
      "topics": [
        "ensembl_tx_biotypes"
      ]
    },
    {
      "page": "get_intron_flanks",
      "title": "Get flanked intronic ranges",
      "topics": [
        "get_intron_flanks"
      ]
    },
    {
      "page": "knee_plot",
      "title": "Plot the transposed knee plot and inflection point",
      "topics": [
        "get_inflection",
        "get_knee_df",
        "knee_plot"
      ]
    },
    {
      "page": "get_velocity_files",
      "title": "Get files required for RNA velocity with bustools",
      "topics": [
        "get_velocity_files",
        "get_velocity_files,character-method",
        "get_velocity_files,EnsDb-method",
        "get_velocity_files,GRanges-method",
        "get_velocity_files,TxDb-method"
      ]
    },
    {
      "page": "make_sparse_matrix",
      "title": "Convert the Output of 'kallisto bus' into Gene by Gell Matrix",
      "topics": [
        "make_sparse_matrix"
      ]
    },
    {
      "page": "match_style",
      "title": "Match chromosome naming styles of annotation and genome",
      "topics": [
        "match_style"
      ]
    },
    {
      "page": "read_count_output",
      "title": "Read matrix along with barcode and gene names",
      "topics": [
        "read_count_output"
      ]
    },
    {
      "page": "read_velocity_output",
      "title": "Read intronic and exonic matrices into R",
      "topics": [
        "read_velocity_output"
      ]
    },
    {
      "page": "refseq_gff_mcols",
      "title": "Tags in the attributes field of RefSeq GFF files",
      "topics": [
        "refseq_gff_mcols"
      ]
    },
    {
      "page": "save_tr2g_bustools",
      "title": "Save transcript to gene file for use in 'bustools'",
      "topics": [
        "save_tr2g_bustools"
      ]
    },
    {
      "page": "sort_tr2g",
      "title": "Sort transcripts to the same order as in kallisto index",
      "concept": [
        "functions to retrieve transcript and gene info"
      ],
      "topics": [
        "sort_tr2g"
      ]
    },
    {
      "page": "species2dataset",
      "title": "Convert Latin species name to dataset name",
      "topics": [
        "species2dataset"
      ]
    },
    {
      "page": "standardize_tags",
      "title": "Standardize GRanges field names",
      "topics": [
        "standardize_tags"
      ]
    },
    {
      "page": "sub_annot",
      "title": "Remove chromosomes in anotation absent from genome",
      "topics": [
        "sub_annot"
      ]
    },
    {
      "page": "subset_annot",
      "title": "Subset genome annotation",
      "topics": [
        "subset_annot",
        "subset_annot,BSgenome-method",
        "subset_annot,DNAStringSet-method"
      ]
    },
    {
      "page": "tr2g_EnsDb",
      "title": "Get transcript and gene info from EnsDb objects",
      "concept": [
        "functions to retrieve transcript and gene info"
      ],
      "topics": [
        "tr2g_EnsDb"
      ]
    },
    {
      "page": "tr2g_ensembl",
      "title": "Get transcript and gene info from Ensembl",
      "concept": [
        "functions to retrieve transcript and gene info"
      ],
      "topics": [
        "tr2g_ensembl"
      ]
    },
    {
      "page": "tr2g_fasta",
      "title": "Get transcript and gene info from names in FASTA files",
      "concept": [
        "functions to retrieve transcript and gene info"
      ],
      "topics": [
        "tr2g_fasta"
      ]
    },
    {
      "page": "tr2g_gff3",
      "title": "Get transcript and gene info from GFF3 file",
      "concept": [
        "functions to retrieve transcript and gene info"
      ],
      "topics": [
        "tr2g_gff3"
      ]
    },
    {
      "page": "tr2g_GRanges",
      "title": "Get transcript and gene info from GRanges",
      "topics": [
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