Vignette of the a4Classif package
Introduction
This document explains the functionalities available in the a4Classif package.
This package contains for classification of Affymetrix microarray
data, stored in an ExpressionSet. This package integrates
within the Automated Affymetrix Array Analysis suite of packages.
## Loading required package: a4Core## Loading required package: a4Preproc##
## a4Classif version 1.55.0## Loading required package: Biobase## Loading required package: BiocGenerics##
## Attaching package: 'BiocGenerics'## The following objects are masked from 'package:stats':
##
## IQR, mad, sd, var, xtabs## The following objects are masked from 'package:base':
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## Filter, Find, Map, Position, Reduce, anyDuplicated, aperm, append,
## as.data.frame, basename, cbind, colnames, dirname, do.call,
## duplicated, eval, evalq, get, grep, grepl, intersect, is.unsorted,
## lapply, mapply, match, mget, order, paste, pmax, pmax.int, pmin,
## pmin.int, rank, rbind, rownames, sapply, saveRDS, setdiff, table,
## tapply, union, unique, unsplit, which.max, which.min## Welcome to Bioconductor
##
## Vignettes contain introductory material; view with
## 'browseVignettes()'. To cite Bioconductor, see
## 'citation("Biobase")', and for packages 'citation("pkgname")'.To demonstrate the functionalities of the package, the
ALL dataset is used. The genes are annotated thanks to the
addGeneInfo utility function of the a4Preproc
package.
## Loading required package: hgu95av2.db## Loading required package: AnnotationDbi## Loading required package: stats4## Loading required package: IRanges## Loading required package: S4Vectors##
## Attaching package: 'S4Vectors'## The following object is masked from 'package:utils':
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## findMatches## The following objects are masked from 'package:base':
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## I, expand.grid, unname## Loading required package: org.Hs.eg.db## ## Classify microarray data
Lasso regression
resultLasso <- lassoClass(object = ALL, groups = "BTtype")
plot(resultLasso,
label = TRUE,
main = "Lasso coefficients in relation to degree of penalization."
)
## The lasso selected 16 genes. The top 15 genes are:
##
## Gene Coefficient
## 38319_at CD3D 0.95966733
## 35016_at CD74 -0.60928095
## 38147_at SH2D1A 0.49240967
## 35792_at MGLL 0.46856925
## 37563_at SRGAP3 0.26648240
## 38917_at YME1L1 0.25100075
## 40278_at GGA2 -0.25017550
## 41164_at IGHM -0.12387272
## 41409_at THEMIS2 -0.10581122
## 38242_at BLNK -0.10309606
## 35523_at HPGDS 0.10169706
## 38949_at PRKCQ 0.07832802
## 33316_at TOX 0.06963509
## 33839_at ITPR2 0.05801832
## 40570_at FOXO1 -0.04858863PAM regression
resultPam <- pamClass(object = ALL, groups = "BTtype")
plot(resultPam,
main = "Pam misclassification error versus number of genes."
)
## Pam selected 1 genes. The top 15 genes are:
##
## GeneSymbol B.score T.score av.rank.in.CV prop.selected.in.CV
## 38319_at CD3D -0.1693 0.4875 1 1## predicted
## true B T
## B 95 0
## T 1 32Random forest
# select only a subset of the data for computation time reason
ALLSubset <- ALL[sample.int(n = nrow(ALL), size = 100, replace = FALSE), ]
resultRf <- rfClass(object = ALLSubset, groups = "BTtype")
plot(resultRf)
## Random forest selected 26 genes. The top 15 genes are:
##
## GeneSymbol
## 31609_s_at PCOLCE
## 32542_at FHL1
## 32562_at ENG
## 32676_at ALDH6A1
## 32847_at MYLK
## 33190_g_at <NA>
## 33376_at <NA>
## 33484_at RNF2
## 33824_at KRT8
## 33837_at SRP72
## 34156_i_at <NA>
## 34595_at MYO1A
## 35423_at GRM5
## 36997_at AK1
## 38011_at URI1
Appendix
Session information
## R version 4.4.1 (2024-06-14)
## Platform: x86_64-pc-linux-gnu
## Running under: Ubuntu 24.04.1 LTS
##
## Matrix products: default
## BLAS: /usr/lib/x86_64-linux-gnu/openblas-pthread/libblas.so.3
## LAPACK: /usr/lib/x86_64-linux-gnu/openblas-pthread/libopenblasp-r0.3.26.so; LAPACK version 3.12.0
##
## locale:
## [1] LC_CTYPE=en_US.UTF-8 LC_NUMERIC=C LC_TIME=en_US.UTF-8 LC_COLLATE=C LC_MONETARY=en_US.UTF-8 LC_MESSAGES=en_US.UTF-8 LC_PAPER=en_US.UTF-8 LC_NAME=C LC_ADDRESS=C LC_TELEPHONE=C LC_MEASUREMENT=en_US.UTF-8 LC_IDENTIFICATION=C
##
## time zone: Etc/UTC
## tzcode source: system (glibc)
##
## attached base packages:
## [1] stats4 stats graphics grDevices utils datasets methods base
##
## other attached packages:
## [1] hgu95av2.db_3.13.0 org.Hs.eg.db_3.20.0 AnnotationDbi_1.67.0 IRanges_2.39.2 S4Vectors_0.43.2 ALL_1.47.0 Biobase_2.65.1 BiocGenerics_0.51.3 a4Classif_1.55.0 a4Preproc_1.53.0 a4Core_1.53.0 rmarkdown_2.28
##
## loaded via a namespace (and not attached):
## [1] sass_0.4.9 varSelRF_0.7-8 shape_1.4.6.1 RSQLite_2.3.7 lattice_0.22-6 digest_0.6.37 evaluate_1.0.1 grid_4.4.1 iterators_1.0.14 fastmap_1.2.0 blob_1.2.4 foreach_1.5.2 jsonlite_1.8.9 glmnet_4.1-8 Matrix_1.7-1 GenomeInfoDb_1.41.2 DBI_1.2.3 survival_3.7-0 httr_1.4.7 UCSC.utils_1.1.0
## [21] Biostrings_2.73.2 codetools_0.2-20 jquerylib_0.1.4 cli_3.6.3 crayon_1.5.3 rlang_1.1.4 XVector_0.45.0 pamr_1.57 bit64_4.5.2 splines_4.4.1 cachem_1.1.0 yaml_2.3.10 tools_4.4.1 parallel_4.4.1 memoise_2.0.1 GenomeInfoDbData_1.2.13 ROCR_1.0-11 png_0.1-8 buildtools_1.0.0 vctrs_0.6.5
## [41] R6_2.5.1 lifecycle_1.0.4 zlibbioc_1.51.2 KEGGREST_1.45.1 randomForest_4.7-1.2 bit_4.5.0 cluster_2.1.6 pkgconfig_2.0.3 bslib_0.8.0 Rcpp_1.0.13 highr_0.11 xfun_0.48 sys_3.4.3 knitr_1.48 htmltools_0.5.8.1 maketools_1.3.1 compiler_4.4.1