Package: OSCA.advanced Title: Advanced Single-Cell Analysis Version: 1.21.2 Date: 2026-07-28 Authors@R: c( person('Robert', 'Amezquita', role = 'aut'), person('Aaron', 'Lun', role = 'aut'), person('Stephanie', 'Hicks', role = 'aut'), person('Raphael', 'Gottardo', role = 'aut'), person('Ludwig', 'Geistlinger', role = 'cre', email = "ludwig_geistlinger@hms.harvard.edu"), person('Peter', 'Hickey', role = 'ctb') ) Description: Deploys the advanced analysis chapters for the "Orchestrating Single Cell Analysis with Bioconductor" book. This describes the more complex steps of a single-cell RNA-seq analysis ranging from doublet detection, cell cycle assignment, specific steps for processing droplet data, nuclei-specific analyses, trajectory analyses, integrated analyses with protein abundances, and interactive visualization. It also elaborates on some of the basic analysis steps, focusing on alternative strategies and theoretical considerations. It is intended for readers who are already familiar with basic single-cell analyses, possibly after reading some of the prior books in this collection. Depends: AnnotationHub, batchelor, BiocFileCache, BiocNeighbors, BiocParallel, BiocSingular, bluster, clustree, corral, densvis, DropletTestFiles, DropletUtils, edgeR, ggbeeswarm, ggplot2, gridExtra, igraph, iSEE, limma, msigdbr, mumosa, org.Mm.eg.db, pheatmap, rebook, rmarkdown, Rtsne, scater, scDblFinder, scPCA, scran, scrapper, scRNAseq (>= 2.17.9), scuttle, SingleR, slingshot, snifter, TENxBrainData, tradeSeq, TSCAN, velociraptor, viridis Imports: OSCA.workflows, OSCA.basic, OSCA.multisample Suggests: rmarkdown, knitr VignetteBuilder: knitr License: CC BY 4.0 URL: https://github.com/OSCA-source/OSCA.advanced biocViews: ImmunoOncologyWorkflow, Workflow, SingleCellWorkflow Config/pak/sysreqs: cmake libfontconfig1-dev libfreetype6-dev libglpk-dev make libmagick++-dev gsfonts libbz2-dev libicu-dev liblzma-dev libpng-dev libuv1-dev libxml2-dev libssl-dev perl python3 libnode-dev xz-utils zlib1g-dev Repository: https://bioc.r-universe.dev Date/Publication: 2026-07-28 09:26:32 UTC RemoteUrl: https://github.com/bioc/OSCA.advanced RemoteRef: HEAD RemoteSha: 2f1050180630665e196962cde3c2742ddd066a51 NeedsCompilation: no Packaged: 2026-10-10 10:56:27 UTC; root Author: Robert Amezquita [aut], Aaron Lun [aut], Stephanie Hicks [aut], Raphael Gottardo [aut], Ludwig Geistlinger [cre], Peter Hickey [ctb] Maintainer: Ludwig Geistlinger