{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (O4)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].gFQQILAGEYDHLPEQAFYMVGPIEEAVAk.[A]","Modifications":"N-Term(TMT6plex); K30(TMT6plex)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":1270.32495,"MH...Da.":3808.9603,"Theo..MH...Da.":3808.96605,"DeltaM..ppm.":-1.51,"Deltam.z..Da.":-0.00192,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":47.95559,"Average.Reporter.S.N":8.7,"Ion.Inject.Time..ms.":50,"RT..min.":212.2487,"First.Scan":112815,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_03.raw","File.ID":"F1","Abundance..126":2548.32609,"Abundance..127N":3231.92942,"Abundance..127C":2760.83874,"Abundance..128N":4111.6388,"Abundance..128C":3127.25385,"Abundance..129N":1874.16284,"Abundance..129C":2831.42252,"Abundance..130N":2298.40137,"Abundance..130C":3798.87622,"Abundance..131":3739.06677,"Ions.Score":90,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":7.03867e-09,"Percolator.q.Value":0,"Percolator.PEP":0.00001}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (K2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].qYPWGVAEVENGEHcDFTILr.[N]","Modifications":"N-Term(TMT6plex); C15(Carbamidomethyl); R21(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q16181","Master.Protein.Descriptions":"Septin-7 OS=Homo sapiens GN=SEPT7 PE=1 SV=2","Protein.Accessions":"Q16181","Protein.Descriptions":"Septin-7 OS=Homo sapiens GN=SEPT7 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":920.44928,"MH...Da.":2759.33329,"Theo..MH...Da.":2759.33243,"DeltaM..ppm.":0.31,"Deltam.z..Da.":0.00028,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":9.37751,"Average.Reporter.S.N":8.1,"Ion.Inject.Time..ms.":3.242,"RT..min.":164.7507,"First.Scan":87392,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_03.raw","File.ID":"F5","Abundance..126":22861.765,"Abundance..127N":25817.94579,"Abundance..127C":23349.49764,"Abundance..128N":29449.60878,"Abundance..128C":25995.9292,"Abundance..129N":22955.7695,"Abundance..129C":30578.97099,"Abundance..130N":30660.4878,"Abundance..130C":38728.85268,"Abundance..131":25047.27979,"Ions.Score":76,"Identity.Strict":24,"Identity.Relaxed":17,"Expectation.Value":6.29863e-08,"Percolator.q.Value":0,"Percolator.PEP":3.349e-07}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (K2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].dkPSVEPVEEYDYEDLk.[E]","Modifications":"N-Term(TMT6plex); K2(Label); K17(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q9Y450","Master.Protein.Descriptions":"HBS1-like protein OS=Homo sapiens GN=HBS1L PE=1 SV=1","Protein.Accessions":"Q9Y450","Protein.Descriptions":"HBS1-like protein OS=Homo sapiens GN=HBS1L PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"DeltaScore":0.973,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":920.16052,"MH...Da.":2758.46701,"Theo..MH...Da.":2758.46127,"DeltaM..ppm.":2.08,"Deltam.z..Da.":0.00192,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":38.31705,"Average.Reporter.S.N":17.8,"Ion.Inject.Time..ms.":13.596,"RT..min.":143.4534,"First.Scan":74786,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_03.raw","File.ID":"F5","Abundance..126":25504.08265,"Abundance..127N":27740.45018,"Abundance..127C":25144.97442,"Abundance..128N":25754.57874,"Abundance..128C":29923.17631,"Abundance..129N":34097.63744,"Abundance..129C":31650.25481,"Abundance..130N":27632.69202,"Abundance..130C":23886.88088,"Abundance..131":35331.0915,"Ions.Score":74,"Identity.Strict":30,"Identity.Relaxed":23,"Expectation.Value":4.31839e-07,"Percolator.q.Value":0,"Percolator.PEP":9.922e-07}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (F2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[R].hEHQVMLmr.[Q]","Modifications":"N-Term(TMT6plex); M8(Oxidation); R9(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q15233","Master.Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","Protein.Accessions":"Q15233","Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","X..Missed.Cleavages":0,"Charge":4,"DeltaScore":0.525,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":359.68982,"MH...Da.":1435.73745,"Theo..MH...Da.":1435.73751,"DeltaM..ppm.":-0.04,"Deltam.z..Da.":-0.00002,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":21.39004,"Average.Reporter.S.N":36.5,"Ion.Inject.Time..ms.":50,"RT..min.":21.6426,"First.Scan":6458,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture4_02.raw","File.ID":"F10","Abundance..126":13493.22808,"Abundance..127N":14674.48982,"Abundance..127C":11187.89951,"Abundance..128N":12831.49486,"Abundance..128C":13839.42628,"Abundance..129N":12441.35266,"Abundance..129C":13450.88468,"Abundance..130N":14777.84362,"Abundance..130C":13039.99511,"Abundance..131":12057.12132,"Ions.Score":40,"Identity.Strict":25,"Identity.Relaxed":18,"Expectation.Value":0.00034,"Percolator.q.Value":0,"Percolator.PEP":0.00012}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (K2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].dNLTLWTADNAGEEGGEAPQEPQS.[-]","Modifications":"N-Term(TMT6plex)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P31947","Master.Protein.Descriptions":"14-3-3 protein sigma OS=Homo sapiens GN=SFN PE=1 SV=1","Protein.Accessions":"P31947","Protein.Descriptions":"14-3-3 protein sigma OS=Homo sapiens GN=SFN PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":920.0943,"MH...Da.":2758.26834,"Theo..MH...Da.":2758.26413,"DeltaM..ppm.":1.53,"Deltam.z..Da.":0.00141,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":0,"Average.Reporter.S.N":16.7,"Ion.Inject.Time..ms.":6.723,"RT..min.":174.1863,"First.Scan":92950,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_03.raw","File.ID":"F5","Abundance..126":64582.78627,"Abundance..127N":50576.41732,"Abundance..127C":47126.03669,"Abundance..128N":56285.12929,"Abundance..128C":46257.31011,"Abundance..129N":52634.88522,"Abundance..129C":49716.84974,"Abundance..130N":60660.5744,"Abundance..130C":55830.48767,"Abundance..131":40280.57714,"Ions.Score":38,"Identity.Strict":21,"Identity.Relaxed":14,"Expectation.Value":0.00022,"Percolator.q.Value":0,"Percolator.PEP":0.00001}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (K2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].aLVAIGTHDLDTLSGPFTYTAk.[R]","Modifications":"N-Term(TMT6plex); K22(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q9NSD9","Master.Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","Protein.Accessions":"Q9NSD9","Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.9783,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":919.85016,"MH...Da.":2757.53592,"Theo..MH...Da.":2757.53185,"DeltaM..ppm.":1.48,"Deltam.z..Da.":0.00136,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":30.61996,"Average.Reporter.S.N":26.7,"Ion.Inject.Time..ms.":8.958,"RT..min.":176.4863,"First.Scan":94294,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_03.raw","File.ID":"F5","Abundance..126":35404.70915,"Abundance..127N":31905.85204,"Abundance..127C":30993.94128,"Abundance..128N":36854.35114,"Abundance..128C":37506.00133,"Abundance..129N":25703.44415,"Abundance..129C":38626.59827,"Abundance..130N":35447.94244,"Abundance..130C":33788.40934,"Abundance..131":32031.51647,"Ions.Score":46,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.00021,"Percolator.q.Value":0,"Percolator.PEP":0.00007}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (K2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].tDLNPDNLQGGDDLDPNYVLSSr.[V]","Modifications":"N-Term(TMT6plex); R23(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P12277","Master.Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","Protein.Accessions":"P12277","Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":919.78632,"MH...Da.":2757.34439,"Theo..MH...Da.":2757.34042,"DeltaM..ppm.":1.44,"Deltam.z..Da.":0.00133,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":0,"Average.Reporter.S.N":17.5,"Ion.Inject.Time..ms.":7.447,"RT..min.":166.9246,"First.Scan":88681,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_03.raw","File.ID":"F5","Abundance..126":49557.31965,"Abundance..127N":44087.96077,"Abundance..127C":58097.62132,"Abundance..128N":55977.11888,"Abundance..128C":44637.97595,"Abundance..129N":58566.64483,"Abundance..129C":63687.69675,"Abundance..130N":39041.8237,"Abundance..130C":39550.46195,"Abundance..131":52360.92348,"Ions.Score":67,"Identity.Strict":25,"Identity.Relaxed":18,"Expectation.Value":5.97742e-07,"Percolator.q.Value":0,"Percolator.PEP":2.53e-06}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (K2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].wGDAGAEYVVESTGVFTTmEk.[A]","Modifications":"N-Term(TMT6plex); M19(Oxidation); K21(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":920.46259,"MH...Da.":2759.3732,"Theo..MH...Da.":2759.37297,"DeltaM..ppm.":0.09,"Deltam.z..Da.":0.00008,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":19.13594,"Average.Reporter.S.N":10.4,"Ion.Inject.Time..ms.":25.898,"RT..min.":182.4628,"First.Scan":97770,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_03.raw","File.ID":"F5","Abundance..126":11015.70765,"Abundance..127N":10534.92144,"Abundance..127C":12994.96817,"Abundance..128N":7528.86278,"Abundance..128C":10708.13243,"Abundance..129N":11337.53524,"Abundance..129C":10643.97135,"Abundance..130N":8942.8774,"Abundance..130C":8902.60172,"Abundance..131":7864.48684,"Ions.Score":34,"Identity.Strict":26,"Identity.Relaxed":19,"Expectation.Value":0.00177,"Percolator.q.Value":0,"Percolator.PEP":0.00001}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (K2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].eQSIcAAEEQPAEDGQGETNk.[N]","Modifications":"N-Term(TMT6plex); C5(Carbamidomethyl); K21(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q9UGP8","Master.Protein.Descriptions":"Translocation protein SEC63 homolog OS=Homo sapiens GN=SEC63 PE=1 SV=2","Protein.Accessions":"Q9UGP8","Protein.Descriptions":"Translocation protein SEC63 homolog OS=Homo sapiens GN=SEC63 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":919.7771,"MH...Da.":2757.31675,"Theo..MH...Da.":2757.31289,"DeltaM..ppm.":1.4,"Deltam.z..Da.":0.00129,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":52.32669,"Average.Reporter.S.N":34.4,"Ion.Inject.Time..ms.":50,"RT..min.":94.4451,"First.Scan":46008,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_03.raw","File.ID":"F5","Abundance..126":12979.36297,"Abundance..127N":13124.9901,"Abundance..127C":13434.20937,"Abundance..128N":13580.1357,"Abundance..128C":14934.00571,"Abundance..129N":14640.80882,"Abundance..129C":13091.85783,"Abundance..130N":13600.18643,"Abundance..130C":15119.78843,"Abundance..131":14704.22147,"Ions.Score":59,"Identity.Strict":24,"Identity.Relaxed":17,"Expectation.Value":3.13591e-06,"Percolator.q.Value":0,"Percolator.PEP":1.006e-07}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (F2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[K].lVEALcAAGHr.[A]","Modifications":"N-Term(TMT6plex); C6(Carbamidomethyl); R11(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P23919","Master.Protein.Descriptions":"Thymidylate kinase OS=Homo sapiens GN=DTYMK PE=1 SV=4","Protein.Accessions":"P23919","Protein.Descriptions":"Thymidylate kinase OS=Homo sapiens GN=DTYMK PE=1 SV=4","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":479.26865,"MH...Da.":1435.79139,"Theo..MH...Da.":1435.79165,"DeltaM..ppm.":-0.19,"Deltam.z..Da.":-0.00009,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":9.02354,"Average.Reporter.S.N":27.9,"Ion.Inject.Time..ms.":2.34,"RT..min.":103.7277,"First.Scan":50428,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture4_02.raw","File.ID":"F10","Abundance..126":96204.98727,"Abundance..127N":88973.06296,"Abundance..127C":78953.24917,"Abundance..128N":103902.66664,"Abundance..128C":126369.91139,"Abundance..129N":112236.35327,"Abundance..129C":114380.98719,"Abundance..130N":107335.9656,"Abundance..130C":116656.16323,"Abundance..131":137727.76908,"Ions.Score":58,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":0.00001,"Percolator.q.Value":0,"Percolator.PEP":3.456e-06}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (K2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].wGDAGAEYVVESTGVFTTmEk.[A]","Modifications":"N-Term(TMT6plex); M19(Oxidation); K21(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":920.46454,"MH...Da.":2759.37906,"Theo..MH...Da.":2759.37297,"DeltaM..ppm.":2.21,"Deltam.z..Da.":0.00203,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":0,"Average.Reporter.S.N":15.4,"Ion.Inject.Time..ms.":12.489,"RT..min.":190.8828,"First.Scan":102611,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_03.raw","File.ID":"F5","Abundance..126":14980.5393,"Abundance..127N":13139.96509,"Abundance..127C":16418.04758,"Abundance..128N":16965.95013,"Abundance..128C":14269.79603,"Abundance..129N":19259.3219,"Abundance..129C":16210.01903,"Abundance..130N":16411.22238,"Abundance..130C":15404.10632,"Abundance..131":14501.4911,"Ions.Score":41,"Identity.Strict":26,"Identity.Relaxed":19,"Expectation.Value":0.00035,"Percolator.q.Value":0,"Percolator.PEP":4.267e-06}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (K2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].wGDAGAEYVVESTGVFTTmEk.[A]","Modifications":"N-Term(TMT6plex); M19(Oxidation); K21(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":920.46484,"MH...Da.":2759.37998,"Theo..MH...Da.":2759.37297,"DeltaM..ppm.":2.54,"Deltam.z..Da.":0.00234,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":11.7404,"Average.Reporter.S.N":10.2,"Ion.Inject.Time..ms.":10.504,"RT..min.":190.9407,"First.Scan":102644,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_03.raw","File.ID":"F5","Abundance..126":9750.23803,"Abundance..127N":10862.17985,"Abundance..127C":13572.60572,"Abundance..128N":9127.56361,"Abundance..128C":11890.64659,"Abundance..129N":11216.1744,"Abundance..129C":8625.02928,"Abundance..130N":9611.10789,"Abundance..130C":14359.35986,"Abundance..131":10903.31848,"Ions.Score":42,"Identity.Strict":26,"Identity.Relaxed":19,"Expectation.Value":0.0003,"Percolator.q.Value":0,"Percolator.PEP":0.00003}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (K2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].drLPLAVVGSNTIIEVNGk.[R]","Modifications":"N-Term(TMT6plex); R2(Label:13C(6)15N(4)); K19(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q16181","Master.Protein.Descriptions":"Septin-7 OS=Homo sapiens GN=SEPT7 PE=1 SV=2","Protein.Accessions":"Q16181","Protein.Descriptions":"Septin-7 OS=Homo sapiens GN=SEPT7 PE=1 SV=2","X..Missed.Cleavages":1,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":824.49628,"MH...Da.":2471.47428,"Theo..MH...Da.":2471.47165,"DeltaM..ppm.":1.06,"Deltam.z..Da.":0.00088,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":8.94037,"Average.Reporter.S.N":28.9,"Ion.Inject.Time..ms.":7.777,"RT..min.":157.361,"First.Scan":83018,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_03.raw","File.ID":"F5","Abundance..126":53264.66575,"Abundance..127N":54174.47118,"Abundance..127C":60587.04785,"Abundance..128N":68940.08591,"Abundance..128C":54171.75746,"Abundance..129N":65963.94022,"Abundance..129C":60179.97101,"Abundance..130N":48506.09121,"Abundance..130C":53094.15963,"Abundance..131":54385.89243,"Ions.Score":80,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":8.05995e-08,"Percolator.q.Value":0,"Percolator.PEP":4.529e-08}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (F2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].fEDYLNAESr.[V]","Modifications":"N-Term(TMT6plex); R10(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q16181","Master.Protein.Descriptions":"Septin-7 OS=Homo sapiens GN=SEPT7 PE=1 SV=2","Protein.Accessions":"Q16181","Protein.Descriptions":"Septin-7 OS=Homo sapiens GN=SEPT7 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":0.9592,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":741.86938,"MH...Da.":1482.73149,"Theo..MH...Da.":1482.73016,"DeltaM..ppm.":0.9,"Deltam.z..Da.":0.00067,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":30.74999,"Average.Reporter.S.N":57.3,"Ion.Inject.Time..ms.":10.279,"RT..min.":136.8967,"First.Scan":69441,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture4_02.raw","File.ID":"F10","Abundance..126":31275.01129,"Abundance..127N":30779.26772,"Abundance..127C":27129.07657,"Abundance..128N":32250.67357,"Abundance..128C":31559.90867,"Abundance..129N":32130.59037,"Abundance..129C":28166.68749,"Abundance..130N":32339.52113,"Abundance..130C":32821.39484,"Abundance..131":29223.61365,"Ions.Score":49,"Identity.Strict":22,"Identity.Relaxed":15,"Expectation.Value":0.00003,"Percolator.q.Value":0,"Percolator.PEP":0.00003}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (F2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].irPFAVAAVLr.[N]","Modifications":"N-Term(TMT6plex); R2(Label:13C(6)15N(4)); R11(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q9NSD9","Master.Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","Protein.Accessions":"Q9NSD9","Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","X..Missed.Cleavages":1,"Charge":3,"DeltaScore":0.8182,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":487.9838,"MH...Da.":1461.93683,"Theo..MH...Da.":1461.93701,"DeltaM..ppm.":-0.12,"Deltam.z..Da.":-0.00006,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":13.9477,"Average.Reporter.S.N":13.9,"Ion.Inject.Time..ms.":9.978,"RT..min.":134.8947,"First.Scan":68303,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture4_02.raw","File.ID":"F10","Abundance..126":21637.99762,"Abundance..127N":26014.63852,"Abundance..127C":17851.65101,"Abundance..128N":26433.48244,"Abundance..128C":19987.64442,"Abundance..129N":15569.78947,"Abundance..129C":24974.01855,"Abundance..130N":22512.10951,"Abundance..130C":20114.99471,"Abundance..131":22964.31285,"Ions.Score":33,"Identity.Strict":27,"Identity.Relaxed":20,"Expectation.Value":0.00305,"Percolator.q.Value":0,"Percolator.PEP":0.00005}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (K2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[R].dQEGQDVLLFIDNIFr.[F]","Modifications":"N-Term(TMT6plex); R16(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":721.05151,"MH...Da.":2161.13999,"Theo..MH...Da.":2161.13662,"DeltaM..ppm.":1.56,"Deltam.z..Da.":0.00112,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":15.68833,"Average.Reporter.S.N":32.3,"Ion.Inject.Time..ms.":50,"RT..min.":231.7913,"First.Scan":123806,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_03.raw","File.ID":"F5","Abundance..126":11616.41477,"Abundance..127N":8577.73818,"Abundance..127C":11924.48732,"Abundance..128N":10640.36517,"Abundance..128C":12594.22454,"Abundance..129N":10793.90526,"Abundance..129C":10702.13804,"Abundance..130N":11701.42469,"Abundance..130C":10532.38918,"Abundance..131":12527.5637,"Ions.Score":39,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":0.00091,"Percolator.q.Value":0,"Percolator.PEP":0.00002}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (K2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].vIHDNFGIVEGLMTTVHAITATQk.[T]","Modifications":"N-Term(TMT6plex); K24(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":0,"Charge":4,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":766.1817,"MH...Da.":3061.70498,"Theo..MH...Da.":3061.7,"DeltaM..ppm.":1.63,"Deltam.z..Da.":0.00125,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":1.82668,"Average.Reporter.S.N":3.8,"Ion.Inject.Time..ms.":0.184,"RT..min.":192.0523,"First.Scan":103276,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_03.raw","File.ID":"F5","Abundance..126":2201844.46245,"Abundance..127N":2828128.63403,"Abundance..127C":2467759.21202,"Abundance..128N":2330004.94332,"Abundance..129N":1443102.15508,"Abundance..129C":3954612.05101,"Abundance..130N":2054020.71539,"Abundance..130C":2139791.55322,"Abundance..131":1717062.74022,"Ions.Score":85,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":3.04391e-08,"Percolator.q.Value":0,"Percolator.PEP":1.041e-07}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (K2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].kGSITSVQAIYVPADDLTDPAPATTFAHLDATTVLSr.[A]","Modifications":"N-Term(TMT6plex); K1(Label); R37(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":1,"Charge":4,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":1080.58789,"MH...Da.":4319.32973,"Theo..MH...Da.":4319.3294,"DeltaM..ppm.":0.08,"Deltam.z..Da.":0.00008,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":0,"Average.Reporter.S.N":39.5,"Ion.Inject.Time..ms.":18.067,"RT..min.":177.064,"First.Scan":94631,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_03.raw","File.ID":"F5","Abundance..126":80757.75796,"Abundance..127N":84011.09103,"Abundance..127C":75359.77633,"Abundance..128N":76300.21421,"Abundance..128C":92974.50114,"Abundance..129N":70132.60439,"Abundance..129C":72724.70333,"Abundance..130N":91468.84354,"Abundance..130C":86115.0749,"Abundance..131":79412.505,"Ions.Score":89,"Identity.Strict":27,"Identity.Relaxed":20,"Expectation.Value":7.00532e-09,"Percolator.q.Value":0,"Percolator.PEP":3.76e-08}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (K2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].gTGGVDTAAVGGVFDVSNADr.[L]","Modifications":"N-Term(TMT6plex); R21(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P12277","Master.Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","Protein.Accessions":"P12277","Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":735.37335,"MH...Da.":2204.1055,"Theo..MH...Da.":2204.10202,"DeltaM..ppm.":1.58,"Deltam.z..Da.":0.00116,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":40.25056,"Average.Reporter.S.N":21,"Ion.Inject.Time..ms.":3.706,"RT..min.":148.8459,"First.Scan":77960,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_03.raw","File.ID":"F5","Abundance..126":81060.32222,"Abundance..127N":80890.81619,"Abundance..127C":70874.44107,"Abundance..128N":87123.6043,"Abundance..128C":72954.97992,"Abundance..129N":83316.56016,"Abundance..129C":71028.86862,"Abundance..130N":89944.06876,"Abundance..130C":80275.226,"Abundance..131":74093.21076,"Ions.Score":71,"Identity.Strict":26,"Identity.Relaxed":19,"Expectation.Value":3.50312e-07,"Percolator.q.Value":0,"Percolator.PEP":4.56e-07}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (K2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].iMDPNIVGSEHYDVAr.[G]","Modifications":"N-Term(TMT6plex); R16(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":685.68488,"MH...Da.":2055.04007,"Theo..MH...Da.":2055.04061,"DeltaM..ppm.":-0.26,"Deltam.z..Da.":-0.00018,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":8.17617,"Average.Reporter.S.N":17.3,"Ion.Inject.Time..ms.":2.623,"RT..min.":129.7298,"First.Scan":66711,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_03.raw","File.ID":"F5","Abundance..126":117594.98179,"Abundance..127N":118066.52022,"Abundance..127C":105035.84566,"Abundance..128N":171987.36104,"Abundance..128C":108108.13738,"Abundance..129N":74739.47941,"Abundance..129C":116267.30111,"Abundance..130N":136929.78218,"Abundance..130C":134705.52026,"Abundance..131":128504.59377,"Ions.Score":31,"Identity.Strict":26,"Identity.Relaxed":19,"Expectation.Value":0.00361,"Percolator.q.Value":0,"Percolator.PEP":0.0001}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (F2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].iMNVIGEPIDEr.[G]","Modifications":"N-Term(TMT6plex); R12(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":812.94409,"MH...Da.":1624.88091,"Theo..MH...Da.":1624.88053,"DeltaM..ppm.":0.23,"Deltam.z..Da.":0.00019,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":0,"Average.Reporter.S.N":40.6,"Ion.Inject.Time..ms.":8.553,"RT..min.":154.879,"First.Scan":79689,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture4_02.raw","File.ID":"F10","Abundance..126":88225.60771,"Abundance..127N":97601.74011,"Abundance..127C":94370.25734,"Abundance..128N":88530.09613,"Abundance..128C":81994.96844,"Abundance..129N":91170.38609,"Abundance..129C":113076.6071,"Abundance..130N":110022.57024,"Abundance..130C":93971.32552,"Abundance..131":87613.80167,"Ions.Score":91,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":5.621e-09,"Percolator.q.Value":0,"Percolator.PEP":1.143e-06}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (K2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].rGTGGVDTAAVGGVFDVSNADr.[L]","Modifications":"N-Term(TMT6plex); R1(Label:13C(6)15N(4)); R22(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P12277","Master.Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","Protein.Accessions":"P12277","Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"DeltaScore":0.9737,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":790.74371,"MH...Da.":2370.21659,"Theo..MH...Da.":2370.2114,"DeltaM..ppm.":2.19,"Deltam.z..Da.":0.00173,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":0,"Average.Reporter.S.N":20.4,"Ion.Inject.Time..ms.":6.83,"RT..min.":120.4635,"First.Scan":61258,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_03.raw","File.ID":"F5","Abundance..126":78593.08603,"Abundance..127N":57800.19238,"Abundance..127C":64888.79036,"Abundance..128N":62470.77944,"Abundance..128C":69161.03709,"Abundance..129N":54888.32934,"Abundance..129C":74828.69014,"Abundance..130N":60647.47781,"Abundance..130C":60922.36843,"Abundance..131":52434.76552,"Ions.Score":76,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":1.52842e-07,"Percolator.q.Value":0,"Percolator.PEP":8.374e-07}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (F2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].tIAMDGTEGLVr.[G]","Modifications":"N-Term(TMT6plex); R12(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":0.939,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":751.41052,"MH...Da.":1501.81377,"Theo..MH...Da.":1501.81211,"DeltaM..ppm.":1.1,"Deltam.z..Da.":0.00083,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":33.0102,"Average.Reporter.S.N":24.5,"Ion.Inject.Time..ms.":4.849,"RT..min.":126.1575,"First.Scan":63362,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture4_02.raw","File.ID":"F10","Abundance..126":121987.81667,"Abundance..127N":125074.52858,"Abundance..127C":113494.56592,"Abundance..128N":118323.84111,"Abundance..128C":108696.43149,"Abundance..129N":123302.1946,"Abundance..129C":120777.16211,"Abundance..130N":126283.81981,"Abundance..130C":124783.2648,"Abundance..131":107951.10619,"Ions.Score":82,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":5.04063e-08,"Percolator.q.Value":0,"Percolator.PEP":7.646e-07}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (F2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[K].aAGASDVVLYk.[I]","Modifications":"N-Term(TMT6plex); K11(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q9NSD9","Master.Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","Protein.Accessions":"Q9NSD9","Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":0.9138,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":780.4682,"MH...Da.":1559.92912,"Theo..MH...Da.":1559.92886,"DeltaM..ppm.":0.17,"Deltam.z..Da.":0.00013,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":48.66317,"Average.Reporter.S.N":30.1,"Ion.Inject.Time..ms.":20.474,"RT..min.":121.2584,"First.Scan":60628,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture4_02.raw","File.ID":"F10","Abundance..126":46619.42413,"Abundance..127N":38608.86845,"Abundance..127C":39603.75194,"Abundance..128N":41812.88202,"Abundance..128C":40504.47026,"Abundance..129N":50360.96748,"Abundance..129C":49984.64449,"Abundance..130N":45091.21373,"Abundance..130C":43916.60287,"Abundance..131":40456.50423,"Ions.Score":58,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.00001,"Percolator.q.Value":0,"Percolator.PEP":4.953e-07}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (E4)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[R].aIAELGIYPAVDPLDSTSR.[I]","Modifications":"N-Term(TMT6plex)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":739.73792,"MH...Da.":2217.19919,"Theo..MH...Da.":2217.19643,"DeltaM..ppm.":1.25,"Deltam.z..Da.":0.00092,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":17.00003,"Average.Reporter.S.N":9.3,"Ion.Inject.Time..ms.":16.593,"RT..min.":185.8563,"First.Scan":93888,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_01.raw","File.ID":"F11","Abundance..126":7759.66529,"Abundance..127N":8149.47337,"Abundance..127C":9538.9365,"Abundance..128N":8917.59534,"Abundance..128C":7773.16206,"Abundance..129N":7005.00742,"Abundance..129C":10374.9459,"Abundance..130N":8105.70402,"Abundance..130C":9457.53553,"Abundance..131":10921.52139,"Ions.Score":14,"Identity.Strict":30,"Identity.Relaxed":23,"Expectation.Value":0.38101,"Percolator.q.Value":0,"Percolator.PEP":0.0443}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (E4)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].vPTANVSVVDLTcR.[L]","Modifications":"N-Term(TMT6plex); C13(Carbamidomethyl)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":880.48248,"MH...Da.":1759.95769,"Theo..MH...Da.":1759.95739,"DeltaM..ppm.":0.17,"Deltam.z..Da.":0.00015,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":24.2177,"Average.Reporter.S.N":11.6,"Ion.Inject.Time..ms.":30.944,"RT..min.":147.3862,"First.Scan":72304,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_01.raw","File.ID":"F11","Abundance..126":3005.60073,"Abundance..127N":3764.69636,"Abundance..127C":3469.38714,"Abundance..128N":4223.92829,"Abundance..128C":4987.3451,"Abundance..129N":4889.94648,"Abundance..129C":4894.58969,"Abundance..130N":4012.51592,"Abundance..130C":4073.27683,"Abundance..131":3955.31747,"Ions.Score":47,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.00018,"Percolator.q.Value":0,"Percolator.PEP":0.00145}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (E4)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].lISWYDNEFGYSNR.[V]","Modifications":"N-Term(TMT6plex)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":996.98798,"MH...Da.":1992.96868,"Theo..MH...Da.":1992.96531,"DeltaM..ppm.":1.69,"Deltam.z..Da.":0.00168,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":0,"Average.Reporter.S.N":12.4,"Ion.Inject.Time..ms.":23.889,"RT..min.":178.0609,"First.Scan":89660,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_01.raw","File.ID":"F11","Abundance..126":8348.35111,"Abundance..127N":9570.6355,"Abundance..127C":13292.9719,"Abundance..128N":10006.15712,"Abundance..128C":8418.8363,"Abundance..129N":11776.1686,"Abundance..129C":11397.24446,"Abundance..130N":11457.35955,"Abundance..130C":8592.09395,"Abundance..131":10214.26688,"Ions.Score":49,"Identity.Strict":24,"Identity.Relaxed":17,"Expectation.Value":0.00004,"Percolator.q.Value":0,"Percolator.PEP":0.00899}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (E4)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].gALQNIIPASTGAAk.[A]","Modifications":"N-Term(TMT6plex); K15(TMT6plex)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":624.04486,"MH...Da.":1870.12003,"Theo..MH...Da.":1870.11622,"DeltaM..ppm.":2.04,"Deltam.z..Da.":0.00127,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":33.80439,"Average.Reporter.S.N":34.3,"Ion.Inject.Time..ms.":5.334,"RT..min.":142.5948,"First.Scan":69584,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_01.raw","File.ID":"F11","Abundance..126":120847.88155,"Abundance..127N":102516.17887,"Abundance..127C":128794.43403,"Abundance..128N":130531.19116,"Abundance..128C":142123.69738,"Abundance..129N":104136.33613,"Abundance..129C":111410.17684,"Abundance..130N":118166.47592,"Abundance..130C":108319.6204,"Abundance..131":103028.90867,"Ions.Score":26,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":0.01974,"Percolator.q.Value":0,"Percolator.PEP":0.02496}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (E2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].sTPSGFTLDDVIQTGVDNPGHPYIMTVGcVAGDEESYEVFk.[D]","Modifications":"N-Term(TMT6plex); C29(Carbamidomethyl); K41(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P12277","Master.Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","Protein.Accessions":"P12277","Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","X..Missed.Cleavages":0,"Charge":4,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":1225.34607,"MH...Da.":4898.36245,"Theo..MH...Da.":4898.36724,"DeltaM..ppm.":-0.98,"Deltam.z..Da.":-0.0012,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":0,"Average.Reporter.S.N":1.3,"Ion.Inject.Time..ms.":50,"RT..min.":201.1792,"First.Scan":101715,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_01.raw","File.ID":"F11","Abundance..126":502.69546,"Abundance..127C":443.01672,"Abundance..128C":1290.0039,"Abundance..129N":1080.32507,"Abundance..129C":1165.0427,"Ions.Score":30,"Identity.Strict":22,"Identity.Relaxed":15,"Expectation.Value":0.00164,"Percolator.q.Value":0,"Percolator.PEP":4.97e-07}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (L2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].sIEAVHEDIr.[V]","Modifications":"N-Term(TMT6plex); R10(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P23919","Master.Protein.Descriptions":"Thymidylate kinase OS=Homo sapiens GN=DTYMK PE=1 SV=4","Protein.Accessions":"P23919","Protein.Descriptions":"Thymidylate kinase OS=Homo sapiens GN=DTYMK PE=1 SV=4","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.871,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":469.92712,"MH...Da.":1407.76682,"Theo..MH...Da.":1407.76688,"DeltaM..ppm.":-0.04,"Deltam.z..Da.":-0.00002,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":28.82317,"Average.Reporter.S.N":71.7,"Ion.Inject.Time..ms.":3.711,"RT..min.":70.4997,"First.Scan":30857,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_02.raw","File.ID":"F4","Abundance..126":99545.57795,"Abundance..127N":117005.57622,"Abundance..127C":123111.58264,"Abundance..128N":119899.85792,"Abundance..128C":112065.68009,"Abundance..129N":128959.19396,"Abundance..129C":123061.43405,"Abundance..130N":115580.30139,"Abundance..130C":114517.83063,"Abundance..131":128362.51589,"Ions.Score":31,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":0.00486,"Percolator.q.Value":0,"Percolator.PEP":0.00006}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (E2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[K].tVLIMELINNVAk.[A]","Modifications":"N-Term(TMT6plex); K13(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.9333,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":642.06519,"MH...Da.":1924.181,"Theo..MH...Da.":1924.17968,"DeltaM..ppm.":0.69,"Deltam.z..Da.":0.00044,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":21.83725,"Average.Reporter.S.N":164.9,"Ion.Inject.Time..ms.":35.371,"RT..min.":210.3156,"First.Scan":106110,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_01.raw","File.ID":"F11","Abundance..126":71062.32177,"Abundance..127N":71355.73444,"Abundance..127C":71034.95031,"Abundance..128N":72838.98875,"Abundance..128C":58036.53933,"Abundance..129N":67969.23767,"Abundance..129C":71686.54103,"Abundance..130N":73220.95196,"Abundance..130C":70251.95447,"Abundance..131":68349.55563,"Ions.Score":30,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":0.00615,"Percolator.q.Value":0,"Percolator.PEP":0.00001}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (E2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].sMTEAEQQQLIDDHFLFDkPVSPLLLASGMAr.[D]","Modifications":"N-Term(TMT6plex); K19(Label); R32(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P12277","Master.Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","Protein.Accessions":"P12277","Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","X..Missed.Cleavages":1,"Charge":4,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":1016.79358,"MH...Da.":4064.15249,"Theo..MH...Da.":4064.1356,"DeltaM..ppm.":4.16,"Deltam.z..Da.":0.00422,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":46.4776,"Average.Reporter.S.N":13.2,"Ion.Inject.Time..ms.":24.169,"RT..min.":190.5023,"First.Scan":96350,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_01.raw","File.ID":"F11","Abundance..126":4688.42136,"Abundance..127N":4393.74588,"Abundance..127C":5159.86329,"Abundance..128N":4609.95092,"Abundance..128C":3235.52567,"Abundance..129N":5739.11232,"Abundance..129C":4768.11625,"Abundance..130N":5367.95394,"Abundance..130C":4086.26097,"Abundance..131":6470.37748,"Ions.Score":16,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":0.15783,"Percolator.q.Value":0,"Percolator.PEP":7.758e-06}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (E2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].gAMPPAPVPAGTPAPPGPATMMPDGTLGLTPPTTEr.[F]","Modifications":"N-Term(TMT6plex); R36(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q15233","Master.Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","Protein.Accessions":"Q15233","Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":1230.96558,"MH...Da.":3690.88218,"Theo..MH...Da.":3690.87704,"DeltaM..ppm.":1.39,"Deltam.z..Da.":0.00171,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":0,"Average.Reporter.S.N":5.8,"Ion.Inject.Time..ms.":28.298,"RT..min.":182.8394,"First.Scan":92279,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_01.raw","File.ID":"F11","Abundance..126":6831.8858,"Abundance..127N":4718.64143,"Abundance..127C":5426.30445,"Abundance..128N":6101.49656,"Abundance..128C":6924.733,"Abundance..129N":3144.73868,"Abundance..129C":4858.2287,"Abundance..130N":4755.14218,"Abundance..130C":3288.05321,"Abundance..131":8396.34949,"Ions.Score":48,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":0.0001,"Percolator.q.Value":0,"Percolator.PEP":5.317e-08}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (E2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[R].dQEGQDVLLFIDNIFr.[F]","Modifications":"N-Term(TMT6plex); R16(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":721.05096,"MH...Da.":2161.13834,"Theo..MH...Da.":2161.13662,"DeltaM..ppm.":0.8,"Deltam.z..Da.":0.00057,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":0,"Average.Reporter.S.N":37.8,"Ion.Inject.Time..ms.":50,"RT..min.":233.4796,"First.Scan":116547,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_01.raw","File.ID":"F11","Abundance..126":12261.32513,"Abundance..127N":14520.93864,"Abundance..127C":10244.22688,"Abundance..128N":13827.25629,"Abundance..128C":12495.11535,"Abundance..129N":10053.77936,"Abundance..129C":12191.85952,"Abundance..130N":12919.35204,"Abundance..130C":11455.62873,"Abundance..131":15392.48549,"Ions.Score":38,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":0.00103,"Percolator.q.Value":0,"Percolator.PEP":1.722e-06}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (E2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].lGFSEVELVQMVVDGVk.[L]","Modifications":"N-Term(TMT6plex); K17(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P12277","Master.Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","Protein.Accessions":"P12277","Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.8929,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":772.44519,"MH...Da.":2315.32102,"Theo..MH...Da.":2315.31763,"DeltaM..ppm.":1.47,"Deltam.z..Da.":0.00113,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":2.05906,"Average.Reporter.S.N":21.9,"Ion.Inject.Time..ms.":10.342,"RT..min.":224.6667,"First.Scan":112640,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_01.raw","File.ID":"F11","Abundance..126":23267.80833,"Abundance..127N":31914.98998,"Abundance..127C":22153.72615,"Abundance..128N":29416.04518,"Abundance..128C":31256.61828,"Abundance..129N":23460.91652,"Abundance..129C":29541.34916,"Abundance..130N":28525.28302,"Abundance..130C":28162.59014,"Abundance..131":25482.03088,"Ions.Score":28,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.01592,"Percolator.q.Value":0,"Percolator.PEP":7.647e-06}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (K2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].vETELQGVcDTVLGLLDSHLIk.[E]","Modifications":"N-Term(TMT6plex); C9(Carbamidomethyl); K22(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P31947","Master.Protein.Descriptions":"14-3-3 protein sigma OS=Homo sapiens GN=SFN PE=1 SV=1","Protein.Accessions":"P31947","Protein.Descriptions":"14-3-3 protein sigma OS=Homo sapiens GN=SFN PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.957,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":969.2121,"MH...Da.":2905.62174,"Theo..MH...Da.":2905.62001,"DeltaM..ppm.":0.59,"Deltam.z..Da.":0.00057,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":20.07384,"Average.Reporter.S.N":35.1,"Ion.Inject.Time..ms.":13.828,"RT..min.":220.0938,"First.Scan":118285,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_03.raw","File.ID":"F5","Abundance..126":34079.66713,"Abundance..127N":29584.33377,"Abundance..127C":34385.70579,"Abundance..128N":35706.06981,"Abundance..128C":35095.31478,"Abundance..129N":35213.13687,"Abundance..129C":29328.80654,"Abundance..130N":29801.75837,"Abundance..130C":32429.15965,"Abundance..131":33082.84722,"Ions.Score":93,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":4.93178e-09,"Percolator.q.Value":0,"Percolator.PEP":6.105e-09}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (K2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].wGDAGAEYVVESTGVFTTMEk.[A]","Modifications":"N-Term(TMT6plex); K21(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.8485,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":915.13275,"MH...Da.":2743.3837,"Theo..MH...Da.":2743.37805,"DeltaM..ppm.":2.06,"Deltam.z..Da.":0.00188,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":25.50697,"Average.Reporter.S.N":20,"Ion.Inject.Time..ms.":16.075,"RT..min.":208.9776,"First.Scan":112560,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_03.raw","File.ID":"F5","Abundance..126":8476.18087,"Abundance..127N":8964.56167,"Abundance..127C":6645.05009,"Abundance..128N":8356.1623,"Abundance..128C":9953.29271,"Abundance..129N":8467.76629,"Abundance..129C":9156.32808,"Abundance..130N":7987.09758,"Abundance..130C":7224.09098,"Abundance..131":6588.16463,"Ions.Score":33,"Identity.Strict":27,"Identity.Relaxed":20,"Expectation.Value":0.00254,"Percolator.q.Value":0,"Percolator.PEP":0.00004}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (F2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].nLPQYVSNELLEEAFSVFGQVEr.[A]","Modifications":"N-Term(TMT6plex); R23(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q15233","Master.Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","Protein.Accessions":"Q15233","Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":969.83838,"MH...Da.":2907.50058,"Theo..MH...Da.":2907.49652,"DeltaM..ppm.":1.4,"Deltam.z..Da.":0.00135,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":0,"Average.Reporter.S.N":0.5,"Ion.Inject.Time..ms.":3.144,"RT..min.":245.5486,"First.Scan":126938,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture4_02.raw","File.ID":"F10","Abundance..128C":2916.79102,"Abundance..130C":6018.6167,"Ions.Score":74,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":2.6906e-07,"Percolator.q.Value":0,"Percolator.PEP":3.614e-09}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (F2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].tVLIMELINNVAk.[A]","Modifications":"N-Term(TMT6plex); K13(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.875,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":642.06506,"MH...Da.":1924.18064,"Theo..MH...Da.":1924.17968,"DeltaM..ppm.":0.5,"Deltam.z..Da.":0.00032,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":22.32253,"Average.Reporter.S.N":109.6,"Ion.Inject.Time..ms.":39.4,"RT..min.":209.0657,"First.Scan":110020,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture4_02.raw","File.ID":"F10","Abundance..126":81357.55278,"Abundance..127N":72501.06002,"Abundance..127C":65744.47281,"Abundance..128N":78249.73455,"Abundance..128C":74800.93634,"Abundance..129N":79842.41758,"Abundance..129C":81393.08883,"Abundance..130N":80098.74607,"Abundance..130C":69568.26873,"Abundance..131":72283.68036,"Ions.Score":16,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":0.15566,"Percolator.q.Value":0,"Percolator.PEP":0.00007}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (F2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[K].sMTEAEQQQLIDDHFLFDkPVSPLLLASGMAr.[D]","Modifications":"N-Term(TMT6plex); K19(Label); R32(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P12277","Master.Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","Protein.Accessions":"P12277","Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","X..Missed.Cleavages":1,"Charge":4,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":1016.79138,"MH...Da.":4064.1437,"Theo..MH...Da.":4064.1356,"DeltaM..ppm.":1.99,"Deltam.z..Da.":0.00203,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":1.54627,"Average.Reporter.S.N":15.6,"Ion.Inject.Time..ms.":8.477,"RT..min.":189.6253,"First.Scan":99468,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture4_02.raw","File.ID":"F10","Abundance..126":29756.06301,"Abundance..127N":28270.96907,"Abundance..127C":25210.36577,"Abundance..128N":26945.61854,"Abundance..128C":24819.3283,"Abundance..129N":22580.505,"Abundance..129C":27742.80753,"Abundance..130N":27988.01931,"Abundance..130C":28236.60347,"Abundance..131":21423.27676,"Ions.Score":34,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":0.00276,"Percolator.q.Value":0,"Percolator.PEP":3.644e-06}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (F2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].gSITSVQAIYVPADDLTDPAPATTFAHLDATTVLSr.[A]","Modifications":"N-Term(TMT6plex); R36(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":4,"DeltaScore":0.9688,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":989.27069,"MH...Da.":3954.06093,"Theo..MH...Da.":3954.05731,"DeltaM..ppm.":0.92,"Deltam.z..Da.":0.00091,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":1.64367,"Average.Reporter.S.N":10.1,"Ion.Inject.Time..ms.":1.9,"RT..min.":190.0871,"First.Scan":99735,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture4_02.raw","File.ID":"F10","Abundance..126":31862.29389,"Abundance..127N":34742.98502,"Abundance..127C":33887.36087,"Abundance..128N":43150.65205,"Abundance..128C":39647.36567,"Abundance..129N":52651.30416,"Abundance..129C":45511.3777,"Abundance..130N":42240.59781,"Abundance..130C":49180.30358,"Abundance..131":40958.92629,"Ions.Score":32,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":0.00515,"Percolator.q.Value":0,"Percolator.PEP":8.174e-06}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (K2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].tIAMDGTEGLVr.[G]","Modifications":"N-Term(TMT6plex); R12(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":0.9375,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":751.41028,"MH...Da.":1501.81328,"Theo..MH...Da.":1501.81211,"DeltaM..ppm.":0.78,"Deltam.z..Da.":0.00058,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":32.64732,"Average.Reporter.S.N":39.7,"Ion.Inject.Time..ms.":4.784,"RT..min.":125.4046,"First.Scan":64173,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_03.raw","File.ID":"F5","Abundance..126":202073.89361,"Abundance..127N":178051.24703,"Abundance..127C":195929.95152,"Abundance..128N":157723.47329,"Abundance..128C":180641.37515,"Abundance..129N":157518.72398,"Abundance..129C":186142.32713,"Abundance..130N":161550.54898,"Abundance..130C":180662.48686,"Abundance..131":143864.40231,"Ions.Score":64,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":3.11755e-06,"Percolator.q.Value":0,"Percolator.PEP":0.00003}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (F2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[R].dQEGQDVLLFIDNIFr.[F]","Modifications":"N-Term(TMT6plex); R16(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.9714,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":721.05237,"MH...Da.":2161.14255,"Theo..MH...Da.":2161.13662,"DeltaM..ppm.":2.75,"Deltam.z..Da.":0.00198,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":0,"Average.Reporter.S.N":18.5,"Ion.Inject.Time..ms.":50,"RT..min.":232.5545,"First.Scan":121177,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture4_02.raw","File.ID":"F10","Abundance..126":7174.23324,"Abundance..127N":7260.2741,"Abundance..127C":5580.64041,"Abundance..128N":7449.17291,"Abundance..128C":4986.12724,"Abundance..129N":6459.11376,"Abundance..129C":6530.77438,"Abundance..130N":7072.04515,"Abundance..130C":5330.49446,"Abundance..131":6403.5407,"Ions.Score":35,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.00248,"Percolator.q.Value":0,"Percolator.PEP":0.00002}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (K2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[K].lVINGNPITIFQEr.[D]","Modifications":"N-Term(TMT6plex); R14(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.9744,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":618.36322,"MH...Da.":1853.07511,"Theo..MH...Da.":1853.07217,"DeltaM..ppm.":1.59,"Deltam.z..Da.":0.00098,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":6.14124,"Average.Reporter.S.N":57.7,"Ion.Inject.Time..ms.":3.595,"RT..min.":179.6624,"First.Scan":96146,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_03.raw","File.ID":"F5","Abundance..126":470542.92301,"Abundance..127N":519195.77459,"Abundance..127C":538250.82576,"Abundance..128N":533612.25791,"Abundance..128C":422761.99296,"Abundance..129N":490964.35316,"Abundance..129C":487712.80975,"Abundance..130N":477843.81354,"Abundance..130C":528457.86336,"Abundance..131":516106.78365,"Ions.Score":39,"Identity.Strict":30,"Identity.Relaxed":23,"Expectation.Value":0.00151,"Percolator.q.Value":0,"Percolator.PEP":0.00003}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (F2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].lAVEALSSLDGDLAGr.[Y]","Modifications":"N-Term(TMT6plex); R16(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P12277","Master.Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","Protein.Accessions":"P12277","Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.902,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":609.34064,"MH...Da.":1826.00736,"Theo..MH...Da.":1826.00963,"DeltaM..ppm.":-1.24,"Deltam.z..Da.":-0.00076,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":58.26263,"Average.Reporter.S.N":38.9,"Ion.Inject.Time..ms.":4.678,"RT..min.":181.8176,"First.Scan":95095,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture4_02.raw","File.ID":"F10","Abundance..126":96833.41158,"Abundance..127N":101784.16767,"Abundance..127C":88316.51804,"Abundance..128N":96354.49878,"Abundance..128C":109863.97468,"Abundance..129N":97423.59139,"Abundance..129C":103566.35935,"Abundance..130N":96387.13292,"Abundance..130C":90290.17843,"Abundance..131":100405.89628,"Ions.Score":51,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.00008,"Percolator.q.Value":0,"Percolator.PEP":1.161e-06}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (K2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].lVLEVAQHLGESTVr.[T]","Modifications":"N-Term(TMT6plex); R15(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.8788,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":630.70172,"MH...Da.":1890.09061,"Theo..MH...Da.":1890.08855,"DeltaM..ppm.":1.09,"Deltam.z..Da.":0.00069,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":35.93461,"Average.Reporter.S.N":24.1,"Ion.Inject.Time..ms.":1.676,"RT..min.":156.9387,"First.Scan":82770,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_03.raw","File.ID":"F5","Abundance..126":89208.3389,"Abundance..127N":125281.15079,"Abundance..127C":115774.81746,"Abundance..128N":185670.11044,"Abundance..128C":112652.08595,"Abundance..129N":110501.1674,"Abundance..129C":145548.91231,"Abundance..130N":140930.247,"Abundance..130C":140326.03891,"Abundance..131":133598.06388,"Ions.Score":33,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.00457,"Percolator.q.Value":0,"Percolator.PEP":0.00006}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (F2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].lVLEVAQHLGESTVr.[T]","Modifications":"N-Term(TMT6plex); R15(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.8636,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":630.70135,"MH...Da.":1890.08951,"Theo..MH...Da.":1890.08855,"DeltaM..ppm.":0.51,"Deltam.z..Da.":0.00032,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":19.70068,"Average.Reporter.S.N":14.4,"Ion.Inject.Time..ms.":3.853,"RT..min.":157.3447,"First.Scan":81104,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture4_02.raw","File.ID":"F10","Abundance..126":39378.69131,"Abundance..127N":53671.23517,"Abundance..127C":48593.617,"Abundance..128N":58508.2828,"Abundance..128C":45341.49506,"Abundance..129N":44503.72609,"Abundance..129C":62101.09506,"Abundance..130N":44472.09476,"Abundance..130C":56841.50272,"Abundance..131":46612.6971,"Ions.Score":44,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.00039,"Percolator.q.Value":0,"Percolator.PEP":6.406e-07}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (K2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].fTQAGSEVSALLGr.[I]","Modifications":"N-Term(TMT6plex); R14(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":837.96716,"MH...Da.":1674.92705,"Theo..MH...Da.":1674.92517,"DeltaM..ppm.":1.12,"Deltam.z..Da.":0.00094,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":5.87554,"Average.Reporter.S.N":21.2,"Ion.Inject.Time..ms.":3.327,"RT..min.":162.2699,"First.Scan":85921,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_03.raw","File.ID":"F5","Abundance..126":124459.19476,"Abundance..127N":140369.62127,"Abundance..127C":121994.08988,"Abundance..128N":105663.9882,"Abundance..128C":119291.45261,"Abundance..129N":121288.84493,"Abundance..129C":116460.94417,"Abundance..130N":133155.04793,"Abundance..130C":127263.97343,"Abundance..131":119038.01931,"Ions.Score":86,"Identity.Strict":30,"Identity.Relaxed":23,"Expectation.Value":2.86066e-08,"Percolator.q.Value":0,"Percolator.PEP":7.746e-07}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (F2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].kNEPPLTcPYSLk.[A]","Modifications":"N-Term(TMT6plex); K1(Label); C8(Carbamidomethyl); K13(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q9UGP8","Master.Protein.Descriptions":"Translocation protein SEC63 homolog OS=Homo sapiens GN=SEC63 PE=1 SV=2","Protein.Accessions":"Q9UGP8","Protein.Descriptions":"Translocation protein SEC63 homolog OS=Homo sapiens GN=SEC63 PE=1 SV=2","X..Missed.Cleavages":1,"Charge":3,"DeltaScore":0.9286,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":750.77539,"MH...Da.":2250.31162,"Theo..MH...Da.":2250.31058,"DeltaM..ppm.":0.46,"Deltam.z..Da.":0.00034,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":55.37209,"Average.Reporter.S.N":18.5,"Ion.Inject.Time..ms.":9.675,"RT..min.":117.5905,"First.Scan":58505,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture4_02.raw","File.ID":"F10","Abundance..126":16410.19983,"Abundance..127N":25974.37973,"Abundance..127C":21900.89279,"Abundance..128N":27327.29274,"Abundance..128C":28907.62074,"Abundance..129N":23650.32086,"Abundance..129C":23994.86334,"Abundance..130N":23142.33983,"Abundance..130C":22840.8051,"Abundance..131":21354.25707,"Ions.Score":28,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.01528,"Percolator.q.Value":0,"Percolator.PEP":0.00002}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (F2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[R].aIAELGIYPAVDPLDSTSr.[I]","Modifications":"N-Term(TMT6plex); R19(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":743.07373,"MH...Da.":2227.20664,"Theo..MH...Da.":2227.2047,"DeltaM..ppm.":0.87,"Deltam.z..Da.":0.00065,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":45.28704,"Average.Reporter.S.N":54.4,"Ion.Inject.Time..ms.":13.461,"RT..min.":184.6149,"First.Scan":96693,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture4_02.raw","File.ID":"F10","Abundance..126":97823.14977,"Abundance..127N":93440.88587,"Abundance..127C":82133.39381,"Abundance..128N":100748.84435,"Abundance..128C":102257.26463,"Abundance..129N":90804.57226,"Abundance..129C":88622.90085,"Abundance..130N":109786.56156,"Abundance..130C":81441.23573,"Abundance..131":102109.8385,"Ions.Score":39,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.00106,"Percolator.q.Value":0,"Percolator.PEP":1.568e-06}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (K2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].aHGGYSVFAGVGEr.[T]","Modifications":"N-Term(TMT6plex); R14(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.9643,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":549.28931,"MH...Da.":1645.85337,"Theo..MH...Da.":1645.85234,"DeltaM..ppm.":0.62,"Deltam.z..Da.":0.00034,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":13.09048,"Average.Reporter.S.N":16.7,"Ion.Inject.Time..ms.":1.474,"RT..min.":87.1071,"First.Scan":41732,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_03.raw","File.ID":"F5","Abundance..126":46725.85023,"Abundance..127N":85473.29473,"Abundance..127C":78716.14502,"Abundance..128N":88214.48617,"Abundance..128C":80197.70985,"Abundance..129N":68660.50968,"Abundance..129C":95846.61506,"Abundance..130N":73088.14414,"Abundance..130C":75313.31915,"Abundance..131":90257.90255,"Ions.Score":84,"Identity.Strict":27,"Identity.Relaxed":21,"Expectation.Value":2.40625e-08,"Percolator.q.Value":0,"Percolator.PEP":5.615e-06}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (F2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].iYEFPETDDEEENk.[L]","Modifications":"N-Term(TMT6plex); K14(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q16181","Master.Protein.Descriptions":"Septin-7 OS=Homo sapiens GN=SEPT7 PE=1 SV=2","Protein.Accessions":"Q16181","Protein.Descriptions":"Septin-7 OS=Homo sapiens GN=SEPT7 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":1112.54346,"MH...Da.":2224.07964,"Theo..MH...Da.":2224.07889,"DeltaM..ppm.":0.33,"Deltam.z..Da.":0.00037,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":0,"Average.Reporter.S.N":22.3,"Ion.Inject.Time..ms.":50,"RT..min.":147.2067,"First.Scan":75310,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture4_02.raw","File.ID":"F10","Abundance..126":8019.17778,"Abundance..127N":6668.24099,"Abundance..127C":6917.72748,"Abundance..128N":7372.19766,"Abundance..128C":7056.51199,"Abundance..129N":8410.40344,"Abundance..129C":9240.62619,"Abundance..130N":7282.29488,"Abundance..130C":7115.38345,"Abundance..131":9059.788,"Ions.Score":55,"Identity.Strict":24,"Identity.Relaxed":17,"Expectation.Value":7.9063e-06,"Percolator.q.Value":0,"Percolator.PEP":2.346e-06}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (F2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].nLEGYVGFANLPNQVYr.[K]","Modifications":"N-Term(TMT6plex); R17(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q16181","Master.Protein.Descriptions":"Septin-7 OS=Homo sapiens GN=SEPT7 PE=1 SV=2","Protein.Accessions":"Q16181","Protein.Descriptions":"Septin-7 OS=Homo sapiens GN=SEPT7 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":1097.07947,"MH...Da.":2193.15166,"Theo..MH...Da.":2193.15294,"DeltaM..ppm.":-0.58,"Deltam.z..Da.":-0.00064,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":100,"Average.Reporter.S.N":11.9,"Ion.Inject.Time..ms.":44.206,"RT..min.":169.5735,"First.Scan":88140,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture4_02.raw","File.ID":"F10","Abundance..126":4827.05608,"Abundance..127N":5740.61808,"Abundance..127C":4954.07458,"Abundance..128N":5342.91253,"Abundance..128C":4522.42982,"Abundance..129N":7074.00612,"Abundance..129C":4614.07015,"Abundance..130N":4247.91774,"Abundance..130C":3117.38802,"Abundance..131":5905.5702,"Ions.Score":29,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":0.00808,"Percolator.q.Value":0,"Percolator.PEP":8.561e-07}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (F2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].gTGGVDTAAVGGVFDVSNADr.[L]","Modifications":"N-Term(TMT6plex); R21(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P12277","Master.Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","Protein.Accessions":"P12277","Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":735.37305,"MH...Da.":2204.10459,"Theo..MH...Da.":2204.10202,"DeltaM..ppm.":1.16,"Deltam.z..Da.":0.00085,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":28.80626,"Average.Reporter.S.N":13.3,"Ion.Inject.Time..ms.":6.441,"RT..min.":149.2885,"First.Scan":76484,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture4_02.raw","File.ID":"F10","Abundance..126":28473.43456,"Abundance..127N":27986.57557,"Abundance..127C":22501.19165,"Abundance..128N":18141.44421,"Abundance..128C":32491.1701,"Abundance..129N":27107.87704,"Abundance..129C":29063.97645,"Abundance..130N":20081.99575,"Abundance..130C":24390.57,"Abundance..131":24411.08773,"Ions.Score":48,"Identity.Strict":26,"Identity.Relaxed":19,"Expectation.Value":0.00007,"Percolator.q.Value":0,"Percolator.PEP":4.811e-08}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (K2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[R].vPTANVSVVDLTcr.[L]","Modifications":"N-Term(TMT6plex); C13(Carbamidomethyl); R14(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":0.9853,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":885.48755,"MH...Da.":1769.96782,"Theo..MH...Da.":1769.96565,"DeltaM..ppm.":1.22,"Deltam.z..Da.":0.00108,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":1.67659,"Average.Reporter.S.N":4.2,"Ion.Inject.Time..ms.":0.469,"RT..min.":146.8251,"First.Scan":76765,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_03.raw","File.ID":"F5","Abundance..126":397456.72418,"Abundance..127N":401996.40073,"Abundance..127C":310430.70813,"Abundance..128N":346450.11524,"Abundance..128C":460181.8993,"Abundance..129N":115474.85544,"Abundance..129C":597823.93519,"Abundance..130N":457228.99528,"Abundance..130C":389471.61861,"Abundance..131":311414.70626,"Ions.Score":68,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":1.39155e-06,"Percolator.q.Value":0,"Percolator.PEP":6.455e-06}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (L2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].rGTGGVDTAAVGGVFDVSNADr.[L]","Modifications":"N-Term(TMT6plex); R1(Label:13C(6)15N(4)); R22(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P12277","Master.Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","Protein.Accessions":"P12277","Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":790.742,"MH...Da.":2370.21146,"Theo..MH...Da.":2370.2114,"DeltaM..ppm.":0.02,"Deltam.z..Da.":0.00002,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":0,"Average.Reporter.S.N":37.9,"Ion.Inject.Time..ms.":6.367,"RT..min.":120.7692,"First.Scan":58611,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_02.raw","File.ID":"F4","Abundance..126":84307.22159,"Abundance..127N":75235.808,"Abundance..127C":95315.66629,"Abundance..128N":83361.21405,"Abundance..128C":63343.69068,"Abundance..129N":74626.96104,"Abundance..129C":99435.99688,"Abundance..130N":82258.94776,"Abundance..130C":80236.52998,"Abundance..131":83988.76236,"Ions.Score":74,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":2.37816e-07,"Percolator.q.Value":0,"Percolator.PEP":2.029e-10}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (L2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].lIMVLAGASEFDPQYNk.[D]","Modifications":"N-Term(TMT6plex); K17(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q9UGP8","Master.Protein.Descriptions":"Translocation protein SEC63 homolog OS=Homo sapiens GN=SEC63 PE=1 SV=2","Protein.Accessions":"Q9UGP8","Protein.Descriptions":"Translocation protein SEC63 homolog OS=Homo sapiens GN=SEC63 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":788.10455,"MH...Da.":2362.29911,"Theo..MH...Da.":2362.29722,"DeltaM..ppm.":0.8,"Deltam.z..Da.":0.00063,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":12.89981,"Average.Reporter.S.N":16.3,"Ion.Inject.Time..ms.":11.535,"RT..min.":197.0371,"First.Scan":101293,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_02.raw","File.ID":"F4","Abundance..126":14073.94257,"Abundance..127N":8354.86067,"Abundance..127C":14039.02978,"Abundance..128N":13059.24538,"Abundance..128C":13967.53848,"Abundance..129N":14145.84713,"Abundance..129C":15016.20094,"Abundance..130N":11058.44625,"Abundance..130C":8510.89099,"Abundance..131":15703.32295,"Ions.Score":12,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.58281,"Percolator.q.Value":0,"Percolator.PEP":0.00002}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (D2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].vIHDNFGIVEGLMTTVHAITATQk.[T]","Modifications":"N-Term(TMT6plex); K24(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":0,"Charge":4,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":766.1792,"MH...Da.":3061.69497,"Theo..MH...Da.":3061.7,"DeltaM..ppm.":-1.64,"Deltam.z..Da.":-0.00126,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":18.45378,"Average.Reporter.S.N":12.5,"Ion.Inject.Time..ms.":50,"RT..min.":254.65,"First.Scan":132008,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_02.raw","File.ID":"F12","Abundance..126":3893.31464,"Abundance..127N":3194.93047,"Abundance..127C":2994.74265,"Abundance..128N":5108.01308,"Abundance..128C":3896.22126,"Abundance..129N":5307.42287,"Abundance..129C":5045.30233,"Abundance..130N":4866.8446,"Abundance..130C":2807.8267,"Abundance..131":4502.37294,"Ions.Score":25,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.02907,"Percolator.q.Value":0,"Percolator.PEP":0.00002}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (D4)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[R].vPTANVSVVDLTcR.[L]","Modifications":"N-Term(TMT6plex); C13(Carbamidomethyl)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":0.9524,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":880.48474,"MH...Da.":1759.96221,"Theo..MH...Da.":1759.95739,"DeltaM..ppm.":2.74,"Deltam.z..Da.":0.00241,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":36.50819,"Average.Reporter.S.N":4.6,"Ion.Inject.Time..ms.":7.192,"RT..min.":147.2104,"First.Scan":75873,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_02.raw","File.ID":"F12","Abundance..126":6339.50462,"Abundance..127N":4671.64966,"Abundance..127C":6215.09159,"Abundance..128N":3952.16342,"Abundance..128C":3891.77365,"Abundance..129N":5516.28533,"Abundance..129C":4740.43641,"Abundance..130N":3649.09211,"Abundance..130C":5083.62224,"Abundance..131":4308.96491,"Ions.Score":42,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.00062,"Percolator.q.Value":0,"Percolator.PEP":0.01071}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (D2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[R].iMDPNIVGSEHYDVAr.[G]","Modifications":"N-Term(TMT6plex); R16(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":685.68604,"MH...Da.":2055.04355,"Theo..MH...Da.":2055.04061,"DeltaM..ppm.":1.43,"Deltam.z..Da.":0.00098,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":0,"Average.Reporter.S.N":17.5,"Ion.Inject.Time..ms.":1.965,"RT..min.":130.4883,"First.Scan":66005,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_02.raw","File.ID":"F12","Abundance..126":69138.87197,"Abundance..127N":92364.32625,"Abundance..127C":79549.73314,"Abundance..128N":127232.2509,"Abundance..128C":119758.78786,"Abundance..129N":100736.48119,"Abundance..129C":114269.67285,"Abundance..130N":115542.76338,"Abundance..130C":106421.33558,"Abundance..131":109691.80356,"Ions.Score":49,"Identity.Strict":26,"Identity.Relaxed":19,"Expectation.Value":0.00006,"Percolator.q.Value":0,"Percolator.PEP":1.351e-06}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (D2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].lFVGNLPPDITEEEMr.[K]","Modifications":"N-Term(TMT6plex); R16(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q15233","Master.Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","Protein.Accessions":"Q15233","Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":1050.05054,"MH...Da.":2099.0938,"Theo..MH...Da.":2099.09198,"DeltaM..ppm.":0.87,"Deltam.z..Da.":0.00091,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":0,"Average.Reporter.S.N":10.3,"Ion.Inject.Time..ms.":48.367,"RT..min.":177.1357,"First.Scan":93460,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_02.raw","File.ID":"F12","Abundance..126":10338.90194,"Abundance..127N":10620.48993,"Abundance..127C":10952.08188,"Abundance..128N":7221.98062,"Abundance..128C":11565.31516,"Abundance..129N":11651.73875,"Abundance..129C":9419.87658,"Abundance..130N":8836.30348,"Abundance..130C":9381.35858,"Abundance..131":10837.45008,"Ions.Score":49,"Identity.Strict":27,"Identity.Relaxed":20,"Expectation.Value":0.00007,"Percolator.q.Value":0,"Percolator.PEP":0.00001}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (L2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].smTEAEQQQLIDDHFLFDk.[P]","Modifications":"N-Term(TMT6plex); M2(Oxidation); K19(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P12277","Master.Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","Protein.Accessions":"P12277","Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":926.47009,"MH...Da.":2777.39573,"Theo..MH...Da.":2777.39477,"DeltaM..ppm.":0.35,"Deltam.z..Da.":0.00032,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":54.0212,"Average.Reporter.S.N":9.7,"Ion.Inject.Time..ms.":14.042,"RT..min.":165.9869,"First.Scan":84193,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_02.raw","File.ID":"F4","Abundance..126":7761.80866,"Abundance..127N":8844.27818,"Abundance..127C":10252.19748,"Abundance..128N":9504.26889,"Abundance..128C":11469.77408,"Abundance..129N":9303.33388,"Abundance..129C":8700.9026,"Abundance..130N":13394.30204,"Abundance..130C":13741.23396,"Abundance..131":9116.86267,"Ions.Score":35,"Identity.Strict":27,"Identity.Relaxed":20,"Expectation.Value":0.00171,"Percolator.q.Value":0,"Percolator.PEP":1.869e-07}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (D2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].lISWYDNEFGYSNr.[V]","Modifications":"N-Term(TMT6plex); R14(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":1001.99036,"MH...Da.":2002.97344,"Theo..MH...Da.":2002.97358,"DeltaM..ppm.":-0.07,"Deltam.z..Da.":-0.00007,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":51.58149,"Average.Reporter.S.N":8.6,"Ion.Inject.Time..ms.":10.418,"RT..min.":177.4548,"First.Scan":93646,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_02.raw","File.ID":"F12","Abundance..126":31464.20182,"Abundance..127N":25386.20205,"Abundance..127C":19894.52279,"Abundance..128N":26481.07834,"Abundance..128C":17771.0413,"Abundance..129N":23219.25566,"Abundance..129C":19712.54031,"Abundance..130N":27735.68937,"Abundance..130C":25347.94636,"Abundance..131":17537.16096,"Ions.Score":56,"Identity.Strict":24,"Identity.Relaxed":17,"Expectation.Value":8.13057e-06,"Percolator.q.Value":0,"Percolator.PEP":1.868e-07}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (D2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].qFAPIHAEAPEFMEMSVEQEILVTGIk.[V]","Modifications":"N-Term(TMT6plex); K27(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":4,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":878.46936,"MH...Da.":3510.85561,"Theo..MH...Da.":3510.85082,"DeltaM..ppm.":1.37,"Deltam.z..Da.":0.0012,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":21.20541,"Average.Reporter.S.N":9.2,"Ion.Inject.Time..ms.":21.41,"RT..min.":197.5012,"First.Scan":105055,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_02.raw","File.ID":"F12","Abundance..126":9254.01971,"Abundance..127N":8430.26419,"Abundance..127C":7220.61358,"Abundance..128N":4927.61729,"Abundance..128C":7991.28559,"Abundance..129N":8242.88871,"Abundance..129C":7505.27118,"Abundance..130N":7678.94497,"Abundance..130C":6931.9483,"Abundance..131":7843.08674,"Ions.Score":23,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.04781,"Percolator.q.Value":0,"Percolator.PEP":1.614e-06}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (D2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].sMTEAEQQQLIDDHFLFDkPVSPLLLASGMAr.[D]","Modifications":"N-Term(TMT6plex); K19(Label); R32(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P12277","Master.Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","Protein.Accessions":"P12277","Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","X..Missed.Cleavages":1,"Charge":4,"DeltaScore":0.9487,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":1016.78967,"MH...Da.":4064.13686,"Theo..MH...Da.":4064.1356,"DeltaM..ppm.":0.31,"Deltam.z..Da.":0.00032,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":0,"Average.Reporter.S.N":4.4,"Ion.Inject.Time..ms.":6.874,"RT..min.":190.2364,"First.Scan":100963,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_02.raw","File.ID":"F12","Abundance..126":5230.57071,"Abundance..127N":7281.96613,"Abundance..127C":8794.7366,"Abundance..128N":6987.08443,"Abundance..128C":5431.5221,"Abundance..129N":1745.12279,"Abundance..129C":5070.94164,"Abundance..130N":5898.90783,"Abundance..130C":6592.81056,"Abundance..131":3475.31274,"Ions.Score":39,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":0.00099,"Percolator.q.Value":0,"Percolator.PEP":6.372e-07}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (D2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].gSITSVQAIYVPADDLTDPAPATTFAHLDATTVLSr.[A]","Modifications":"N-Term(TMT6plex); R36(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":1318.69153,"MH...Da.":3954.06003,"Theo..MH...Da.":3954.05731,"DeltaM..ppm.":0.69,"Deltam.z..Da.":0.00091,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":0,"Average.Reporter.S.N":3.7,"Ion.Inject.Time..ms.":17.811,"RT..min.":190.5333,"First.Scan":101138,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_02.raw","File.ID":"F12","Abundance..126":1399.56874,"Abundance..127N":1441.97104,"Abundance..127C":1244.11059,"Abundance..128N":836.14567,"Abundance..128C":1318.66898,"Abundance..129N":1046.63397,"Abundance..129C":1387.657,"Abundance..130N":974.75007,"Abundance..130C":1174.50729,"Abundance..131":1448.48524,"Ions.Score":49,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":0.00009,"Percolator.q.Value":0,"Percolator.PEP":4.979e-08}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (D2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].vIHDNFGIVEGLMTTVHAITATQk.[T]","Modifications":"N-Term(TMT6plex); K24(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":1021.23877,"MH...Da.":3061.70176,"Theo..MH...Da.":3061.7,"DeltaM..ppm.":0.57,"Deltam.z..Da.":0.00059,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":2.95289,"Average.Reporter.S.N":55.2,"Ion.Inject.Time..ms.":5.033,"RT..min.":192.8303,"First.Scan":102441,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_02.raw","File.ID":"F12","Abundance..126":64336.08087,"Abundance..127N":64249.10997,"Abundance..127C":70871.26006,"Abundance..128N":73106.68439,"Abundance..128C":65346.71287,"Abundance..129N":67824.6029,"Abundance..129C":64000.60392,"Abundance..130N":78850.92658,"Abundance..130C":74708.47603,"Abundance..131":77493.7562,"Ions.Score":56,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.00003,"Percolator.q.Value":0,"Percolator.PEP":7.365e-09}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (D2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].vIHDNFGIVEGLMTTVHAITATQk.[T]","Modifications":"N-Term(TMT6plex); K24(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":0,"Charge":4,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":766.18146,"MH...Da.":3061.704,"Theo..MH...Da.":3061.7,"DeltaM..ppm.":1.31,"Deltam.z..Da.":0.001,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":28.78547,"Average.Reporter.S.N":15.2,"Ion.Inject.Time..ms.":7.223,"RT..min.":194.5773,"First.Scan":103427,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_02.raw","File.ID":"F12","Abundance..126":27497.80593,"Abundance..127N":19682.49589,"Abundance..127C":23976.74208,"Abundance..128N":25114.47934,"Abundance..128C":28662.51479,"Abundance..129N":22163.03962,"Abundance..129C":17967.45847,"Abundance..130N":26695.00746,"Abundance..130C":30732.87713,"Abundance..131":26228.56063,"Ions.Score":25,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.02804,"Percolator.q.Value":0,"Percolator.PEP":1.704e-06}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (D2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].dYEFMWNPHLGYILTcPSNLGTGLr.[A]","Modifications":"N-Term(TMT6plex); C16(Carbamidomethyl); R25(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P12277","Master.Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","Protein.Accessions":"P12277","Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":1065.19434,"MH...Da.":3193.56845,"Theo..MH...Da.":3193.5676,"DeltaM..ppm.":0.27,"Deltam.z..Da.":0.00029,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":0,"Average.Reporter.S.N":9.5,"Ion.Inject.Time..ms.":3.02,"RT..min.":190.4241,"First.Scan":101070,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_02.raw","File.ID":"F12","Abundance..126":83811.14059,"Abundance..127N":82750.87451,"Abundance..127C":107857.45705,"Abundance..128N":100901.14303,"Abundance..128C":96425.96494,"Abundance..129N":80369.59933,"Abundance..129C":105443.66854,"Abundance..130N":94190.72013,"Abundance..130C":69325.2797,"Abundance..131":104455.47003,"Ions.Score":66,"Identity.Strict":26,"Identity.Relaxed":19,"Expectation.Value":1.13177e-06,"Percolator.q.Value":0,"Percolator.PEP":1.92e-09}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (L2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].drLPLAVVGSNTIIEVNGk.[R]","Modifications":"N-Term(TMT6plex); R2(Label:13C(6)15N(4)); K19(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q16181","Master.Protein.Descriptions":"Septin-7 OS=Homo sapiens GN=SEPT7 PE=1 SV=2","Protein.Accessions":"Q16181","Protein.Descriptions":"Septin-7 OS=Homo sapiens GN=SEPT7 PE=1 SV=2","X..Missed.Cleavages":1,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":824.49646,"MH...Da.":2471.47483,"Theo..MH...Da.":2471.47165,"DeltaM..ppm.":1.29,"Deltam.z..Da.":0.00106,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":2.9617,"Average.Reporter.S.N":43.8,"Ion.Inject.Time..ms.":10.9,"RT..min.":157.2663,"First.Scan":79194,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_02.raw","File.ID":"F4","Abundance..126":25689.76373,"Abundance..127N":26671.05787,"Abundance..127C":24863.08798,"Abundance..128N":24177.62598,"Abundance..128C":30581.059,"Abundance..129N":30891.02628,"Abundance..129C":29360.06506,"Abundance..130N":27213.66952,"Abundance..130C":29410.61587,"Abundance..131":28811.35226,"Ions.Score":60,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":7.10121e-06,"Percolator.q.Value":0,"Percolator.PEP":1.649e-10}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (L2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].fLSQPFQVAEVFTGHmGk.[L]","Modifications":"N-Term(TMT6plex); M16(Oxidation); K18(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":835.78729,"MH...Da.":2505.34732,"Theo..MH...Da.":2505.34557,"DeltaM..ppm.":0.7,"Deltam.z..Da.":0.00058,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":61.91896,"Average.Reporter.S.N":13.3,"Ion.Inject.Time..ms.":8.154,"RT..min.":175.9983,"First.Scan":89862,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_02.raw","File.ID":"F4","Abundance..126":13326.69102,"Abundance..127N":14414.89424,"Abundance..127C":14902.41345,"Abundance..128N":12271.91784,"Abundance..128C":12698.0856,"Abundance..129N":15053.60745,"Abundance..129C":15196.77089,"Abundance..130N":12425.97988,"Abundance..130C":11332.9644,"Abundance..131":13795.2477,"Ions.Score":24,"Identity.Strict":30,"Identity.Relaxed":23,"Expectation.Value":0.03926,"Percolator.q.Value":0,"Percolator.PEP":0.00002}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (L2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].iPSAVGYQPTLATDMGTMQEr.[I]","Modifications":"N-Term(TMT6plex); R21(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":835.75812,"MH...Da.":2505.2598,"Theo..MH...Da.":2505.25543,"DeltaM..ppm.":1.74,"Deltam.z..Da.":0.00146,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":4.75458,"Average.Reporter.S.N":19.4,"Ion.Inject.Time..ms.":2.118,"RT..min.":161.9815,"First.Scan":81895,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_02.raw","File.ID":"F4","Abundance..126":156686.40424,"Abundance..127N":149159.52093,"Abundance..127C":172958.53657,"Abundance..128N":130799.41881,"Abundance..128C":161494.96323,"Abundance..129N":119125.88164,"Abundance..129C":138254.38271,"Abundance..130N":148931.11189,"Abundance..130C":146393.35593,"Abundance..131":175730.54075,"Ions.Score":82,"Identity.Strict":26,"Identity.Relaxed":19,"Expectation.Value":2.38662e-08,"Percolator.q.Value":0,"Percolator.PEP":3.643e-12}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (E2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].fcTGLTQIETLFk.[S]","Modifications":"N-Term(TMT6plex); C2(Carbamidomethyl); K13(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P12277","Master.Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","Protein.Accessions":"P12277","Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.8919,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":675.38495,"MH...Da.":2024.14029,"Theo..MH...Da.":2024.1382,"DeltaM..ppm.":1.03,"Deltam.z..Da.":0.0007,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":4.08019,"Average.Reporter.S.N":57,"Ion.Inject.Time..ms.":2.946,"RT..min.":196.9543,"First.Scan":99640,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_01.raw","File.ID":"F11","Abundance..126":233054.75917,"Abundance..127N":242825.40382,"Abundance..127C":245796.9904,"Abundance..128N":240368.7132,"Abundance..128C":274196.65269,"Abundance..129N":226405.71522,"Abundance..129C":246255.56678,"Abundance..130N":279835.90043,"Abundance..130C":258975.18789,"Abundance..131":263660.83364,"Ions.Score":37,"Identity.Strict":30,"Identity.Relaxed":23,"Expectation.Value":0.00208,"Percolator.q.Value":0,"Percolator.PEP":8.146e-06}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (E2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[K].nLEGYVGFANLPNQVYr.[K]","Modifications":"N-Term(TMT6plex); R17(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q16181","Master.Protein.Descriptions":"Septin-7 OS=Homo sapiens GN=SEPT7 PE=1 SV=2","Protein.Accessions":"Q16181","Protein.Descriptions":"Septin-7 OS=Homo sapiens GN=SEPT7 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.9706,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":731.72345,"MH...Da.":2193.1558,"Theo..MH...Da.":2193.15294,"DeltaM..ppm.":1.3,"Deltam.z..Da.":0.00095,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":6.15793,"Average.Reporter.S.N":11.3,"Ion.Inject.Time..ms.":5.767,"RT..min.":170.2384,"First.Scan":85291,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_01.raw","File.ID":"F11","Abundance..126":48662.87859,"Abundance..127N":44293.3477,"Abundance..127C":42890.37759,"Abundance..128N":47763.41797,"Abundance..128C":41413.52123,"Abundance..129N":52725.06913,"Abundance..129C":45880.75573,"Abundance..130N":31324.15293,"Abundance..130C":41592.95347,"Abundance..131":35535.55691,"Ions.Score":34,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":0.00327,"Percolator.q.Value":0,"Percolator.PEP":3.109e-07}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (E2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].gTGGVDTAAVGGVFDVSNADr.[L]","Modifications":"N-Term(TMT6plex); R21(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P12277","Master.Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","Protein.Accessions":"P12277","Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":735.37207,"MH...Da.":2204.10166,"Theo..MH...Da.":2204.10202,"DeltaM..ppm.":-0.17,"Deltam.z..Da.":-0.00012,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":38.29248,"Average.Reporter.S.N":15.7,"Ion.Inject.Time..ms.":4.48,"RT..min.":149.7451,"First.Scan":73641,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_01.raw","File.ID":"F11","Abundance..126":38903.83145,"Abundance..127N":33342.97029,"Abundance..127C":31488.12607,"Abundance..128N":41082.58637,"Abundance..128C":40970.68442,"Abundance..129N":32353.56727,"Abundance..129C":38359.24958,"Abundance..130N":31974.45437,"Abundance..130C":34466.43594,"Abundance..131":24277.57197,"Ions.Score":48,"Identity.Strict":25,"Identity.Relaxed":18,"Expectation.Value":0.00007,"Percolator.q.Value":0,"Percolator.PEP":3.032e-11}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (L2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].dLFDPIIEDr.[H]","Modifications":"N-Term(TMT6plex); R10(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P12277","Master.Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","Protein.Accessions":"P12277","Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":0.8077,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":736.39789,"MH...Da.":1471.7885,"Theo..MH...Da.":1471.78694,"DeltaM..ppm.":1.06,"Deltam.z..Da.":0.00078,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":1.26976,"Average.Reporter.S.N":10.6,"Ion.Inject.Time..ms.":3.419,"RT..min.":168.9196,"First.Scan":85869,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_02.raw","File.ID":"F4","Abundance..126":55962.6201,"Abundance..127N":82522.16202,"Abundance..127C":85030.83686,"Abundance..128N":92668.3353,"Abundance..128C":71170.13713,"Abundance..129N":73351.47949,"Abundance..129C":126013.90896,"Abundance..130N":90518.12027,"Abundance..130C":77540.54441,"Abundance..131":69738.4596,"Ions.Score":78,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":1.24813e-07,"Percolator.q.Value":0,"Percolator.PEP":0.00007}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (E2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].lVINGNPITIFQEr.[D]","Modifications":"N-Term(TMT6plex); R14(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.98,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":618.36298,"MH...Da.":1853.07437,"Theo..MH...Da.":1853.07217,"DeltaM..ppm.":1.19,"Deltam.z..Da.":0.00074,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":8.34855,"Average.Reporter.S.N":57.8,"Ion.Inject.Time..ms.":9.724,"RT..min.":180.404,"First.Scan":90950,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_01.raw","File.ID":"F11","Abundance..126":349661.43183,"Abundance..127N":280567.83877,"Abundance..127C":320787.4504,"Abundance..128N":407160.17924,"Abundance..128C":326811.66119,"Abundance..129N":298136.04353,"Abundance..129C":305063.76341,"Abundance..130N":298480.82569,"Abundance..130C":297259.36047,"Abundance..131":294492.95958,"Ions.Score":50,"Identity.Strict":30,"Identity.Relaxed":23,"Expectation.Value":0.00011,"Percolator.q.Value":0,"Percolator.PEP":5.585e-08}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (E2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].lAVEALSSLDGDLAGr.[Y]","Modifications":"N-Term(TMT6plex); R16(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P12277","Master.Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","Protein.Accessions":"P12277","Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":609.34137,"MH...Da.":1826.00956,"Theo..MH...Da.":1826.00963,"DeltaM..ppm.":-0.04,"Deltam.z..Da.":-0.00002,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":60.55663,"Average.Reporter.S.N":34.3,"Ion.Inject.Time..ms.":21.014,"RT..min.":182.3052,"First.Scan":91996,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_01.raw","File.ID":"F11","Abundance..126":23037.05735,"Abundance..127N":26142.84433,"Abundance..127C":17711.78075,"Abundance..128N":25279.69524,"Abundance..128C":24783.31882,"Abundance..129N":26128.17871,"Abundance..129C":25584.49921,"Abundance..130N":25465.42048,"Abundance..130C":20381.21261,"Abundance..131":20722.09971,"Ions.Score":31,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.00752,"Percolator.q.Value":0,"Percolator.PEP":2.432e-07}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (E2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].tTFDEAMADLHTLSEDSYk.[D]","Modifications":"N-Term(TMT6plex); K19(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P31947","Master.Protein.Descriptions":"14-3-3 protein sigma OS=Homo sapiens GN=SFN PE=1 SV=1","Protein.Accessions":"P31947","Protein.Descriptions":"14-3-3 protein sigma OS=Homo sapiens GN=SFN PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":880.77277,"MH...Da.":2640.30375,"Theo..MH...Da.":2640.29947,"DeltaM..ppm.":1.62,"Deltam.z..Da.":0.00143,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":12.31212,"Average.Reporter.S.N":30.1,"Ion.Inject.Time..ms.":12.14,"RT..min.":178.9638,"First.Scan":90162,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_01.raw","File.ID":"F11","Abundance..126":65831.72456,"Abundance..127N":58399.80252,"Abundance..127C":64871.55735,"Abundance..128N":82713.92608,"Abundance..128C":66918.38283,"Abundance..129N":64939.12973,"Abundance..129C":71594.3312,"Abundance..130N":93556.0237,"Abundance..130C":72626.63891,"Abundance..131":74351.91403,"Ions.Score":43,"Identity.Strict":26,"Identity.Relaxed":19,"Expectation.Value":0.00019,"Percolator.q.Value":0,"Percolator.PEP":1.018e-07}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (E2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].hDMAAAGFTEALTFALcSQEDIADk.[L]","Modifications":"N-Term(TMT6plex); C17(Carbamidomethyl); K25(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q9NSD9","Master.Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","Protein.Accessions":"Q9NSD9","Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":1060.19409,"MH...Da.":3178.56772,"Theo..MH...Da.":3178.56806,"DeltaM..ppm.":-0.1,"Deltam.z..Da.":-0.00011,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":68.89565,"Average.Reporter.S.N":5.7,"Ion.Inject.Time..ms.":50,"RT..min.":200.2056,"First.Scan":101259,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_01.raw","File.ID":"F11","Abundance..126":1229.03839,"Abundance..127N":1115.02182,"Abundance..127C":1810.09656,"Abundance..128N":2849.40218,"Abundance..128C":1407.85071,"Abundance..129N":2063.44613,"Abundance..129C":2221.36241,"Abundance..130N":2653.20962,"Abundance..130C":2466.94021,"Abundance..131":1726.78451,"Ions.Score":33,"Identity.Strict":25,"Identity.Relaxed":18,"Expectation.Value":0.00194,"Percolator.q.Value":0,"Percolator.PEP":1.576e-07}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (E2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].dYEFMWNPHLGYILTcPSNLGTGLr.[A]","Modifications":"N-Term(TMT6plex); C16(Carbamidomethyl); R25(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P12277","Master.Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","Protein.Accessions":"P12277","Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":1065.19507,"MH...Da.":3193.57065,"Theo..MH...Da.":3193.5676,"DeltaM..ppm.":0.96,"Deltam.z..Da.":0.00102,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":1.83373,"Average.Reporter.S.N":0.3,"Ion.Inject.Time..ms.":3.385,"RT..min.":191.3084,"First.Scan":96768,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_01.raw","File.ID":"F11","Abundance..130C":26160.50391,"Ions.Score":46,"Identity.Strict":26,"Identity.Relaxed":19,"Expectation.Value":0.00013,"Percolator.q.Value":0,"Percolator.PEP":3.581e-08}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (E2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].sTLINSLFLTDLYSPEYPGPSHr.[I]","Modifications":"N-Term(TMT6plex); R23(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q16181","Master.Protein.Descriptions":"Septin-7 OS=Homo sapiens GN=SEPT7 PE=1 SV=2","Protein.Accessions":"Q16181","Protein.Descriptions":"Septin-7 OS=Homo sapiens GN=SEPT7 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":949.49878,"MH...Da.":2846.48178,"Theo..MH...Da.":2846.48014,"DeltaM..ppm.":0.58,"Deltam.z..Da.":0.00055,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":15.91248,"Average.Reporter.S.N":2.7,"Ion.Inject.Time..ms.":6.865,"RT..min.":189.0001,"First.Scan":95581,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_01.raw","File.ID":"F11","Abundance..126":2239.5067,"Abundance..127N":3528.52201,"Abundance..127C":4468.81954,"Abundance..128N":3767.21803,"Abundance..129N":2778.54165,"Abundance..129C":1167.50306,"Abundance..130N":2301.30128,"Abundance..130C":3051.76294,"Ions.Score":57,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.00002,"Percolator.q.Value":0,"Percolator.PEP":2.543e-10}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (E2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].rVIISAPSADAPMFVMGVNHEk.[Y]","Modifications":"N-Term(TMT6plex); R1(Label:13C(6)15N(4)); K22(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":1,"Charge":4,"DeltaScore":0.963,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":712.15015,"MH...Da.":2845.57876,"Theo..MH...Da.":2845.55876,"DeltaM..ppm.":7.03,"Deltam.z..Da.":0.005,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":25.07833,"Average.Reporter.S.N":147.4,"Ion.Inject.Time..ms.":6.746,"RT..min.":144.9919,"First.Scan":70935,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_01.raw","File.ID":"F11","Abundance..126":1093197.46837,"Abundance..127N":1075211.86663,"Abundance..127C":1264736.00049,"Abundance..128N":1233864.219,"Abundance..128C":1132167.77529,"Abundance..129N":1041317.95407,"Abundance..129C":1179918.11529,"Abundance..130N":1123524.85932,"Abundance..130C":1064584.38238,"Abundance..131":1080067.03421,"Ions.Score":27,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.01757,"Percolator.q.Value":0,"Percolator.PEP":1.686e-06}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (E2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].rVIISAPSADAPMFVmGVNHEk.[Y]","Modifications":"N-Term(TMT6plex); R1(Label:13C(6)15N(4)); M16(Oxidation); K22(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":1,"Charge":4,"DeltaScore":0.3659,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":716.14508,"MH...Da.":2861.55849,"Theo..MH...Da.":2861.55368,"DeltaM..ppm.":1.68,"Deltam.z..Da.":0.0012,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":15.60776,"Average.Reporter.S.N":29.6,"Ion.Inject.Time..ms.":6.942,"RT..min.":136.2348,"First.Scan":66021,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_01.raw","File.ID":"F11","Abundance..126":27957.45695,"Abundance..127N":31248.94686,"Abundance..127C":26757.47449,"Abundance..128N":28525.48736,"Abundance..128C":27321.27086,"Abundance..129N":33425.22416,"Abundance..129C":31626.16967,"Abundance..130N":29929.75875,"Abundance..130C":32563.50956,"Abundance..131":28476.6459,"Ions.Score":41,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.00076,"Percolator.q.Value":0,"Percolator.PEP":1.814e-06}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (E2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].eSDVGFIPTSGLSGENLITr.[S]","Modifications":"N-Term(TMT6plex); R20(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q9Y450","Master.Protein.Descriptions":"HBS1-like protein OS=Homo sapiens GN=HBS1L PE=1 SV=1","Protein.Accessions":"Q9Y450","Protein.Descriptions":"HBS1-like protein OS=Homo sapiens GN=HBS1L PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":1166.11633,"MH...Da.":2331.22539,"Theo..MH...Da.":2331.22689,"DeltaM..ppm.":-0.64,"Deltam.z..Da.":-0.00075,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":34.13987,"Average.Reporter.S.N":10.9,"Ion.Inject.Time..ms.":50,"RT..min.":170.6402,"First.Scan":85522,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_01.raw","File.ID":"F11","Abundance..126":2013.31296,"Abundance..127N":3590.5665,"Abundance..127C":3875.53207,"Abundance..128N":3115.88513,"Abundance..128C":3044.31557,"Abundance..129N":4544.57013,"Abundance..129C":4868.44893,"Abundance..130N":4475.37483,"Abundance..130C":3075.29099,"Abundance..131":4544.44858,"Ions.Score":60,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":8.59266e-06,"Percolator.q.Value":0,"Percolator.PEP":7.311e-09}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (L2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].hEHQVmLmr.[Q]","Modifications":"N-Term(TMT6plex); M6(Oxidation); M8(Oxidation); R9(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q15233","Master.Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","Protein.Accessions":"Q15233","Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","X..Missed.Cleavages":0,"Charge":4,"DeltaScore":0.9062,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":363.68845,"MH...Da.":1451.73195,"Theo..MH...Da.":1451.73242,"DeltaM..ppm.":-0.32,"Deltam.z..Da.":-0.00012,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":20.00229,"Average.Reporter.S.N":22.8,"Ion.Inject.Time..ms.":20.472,"RT..min.":15.2954,"First.Scan":3680,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_02.raw","File.ID":"F4","Abundance..126":8464.25058,"Abundance..127N":9161.74858,"Abundance..127C":8029.15541,"Abundance..128N":6026.33819,"Abundance..128C":6523.74649,"Abundance..129N":7492.74868,"Abundance..129C":7348.66295,"Abundance..130N":7329.98951,"Abundance..130C":7407.08145,"Abundance..131":7882.58499,"Ions.Score":32,"Identity.Strict":24,"Identity.Relaxed":17,"Expectation.Value":0.00202,"Percolator.q.Value":0,"Percolator.PEP":0.00015}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (E2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].kMEMEMEQVFEMk.[V]","Modifications":"N-Term(TMT6plex); K1(Label); K13(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q16181","Master.Protein.Descriptions":"Septin-7 OS=Homo sapiens GN=SEPT7 PE=1 SV=2","Protein.Accessions":"Q16181","Protein.Descriptions":"Septin-7 OS=Homo sapiens GN=SEPT7 PE=1 SV=2","X..Missed.Cleavages":1,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":798.42383,"MH...Da.":2393.25693,"Theo..MH...Da.":2393.25268,"DeltaM..ppm.":1.78,"Deltam.z..Da.":0.00142,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":34.92554,"Average.Reporter.S.N":33.2,"Ion.Inject.Time..ms.":8.365,"RT..min.":166.7584,"First.Scan":83341,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_01.raw","File.ID":"F11","Abundance..126":78769.28811,"Abundance..127N":68112.94176,"Abundance..127C":73123.43516,"Abundance..128N":81235.31887,"Abundance..128C":58771.70096,"Abundance..129N":61510.61423,"Abundance..129C":71619.34115,"Abundance..130N":65981.04903,"Abundance..130C":80421.14024,"Abundance..131":58756.6874,"Ions.Score":25,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.02555,"Percolator.q.Value":0,"Percolator.PEP":0.00001}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (E2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].rGTGGVDTAAVGGVFDVSNADr.[L]","Modifications":"N-Term(TMT6plex); R1(Label:13C(6)15N(4)); R22(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P12277","Master.Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","Protein.Accessions":"P12277","Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":790.74249,"MH...Da.":2370.21292,"Theo..MH...Da.":2370.2114,"DeltaM..ppm.":0.64,"Deltam.z..Da.":0.00051,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":0,"Average.Reporter.S.N":19.2,"Ion.Inject.Time..ms.":1.023,"RT..min.":121.3684,"First.Scan":57861,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_01.raw","File.ID":"F11","Abundance..126":90663.81276,"Abundance..127N":95533.57036,"Abundance..127C":89264.47872,"Abundance..128N":124967.07091,"Abundance..128C":117870.51049,"Abundance..129N":98793.47692,"Abundance..129C":108227.12068,"Abundance..130N":96444.99836,"Abundance..130C":95717.79669,"Abundance..131":94378.70392,"Ions.Score":73,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":3.53852e-07,"Percolator.q.Value":0,"Percolator.PEP":2.927e-09}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (E2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].lIMVLAGASEFDPQYNk.[D]","Modifications":"N-Term(TMT6plex); K17(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q9UGP8","Master.Protein.Descriptions":"Translocation protein SEC63 homolog OS=Homo sapiens GN=SEC63 PE=1 SV=2","Protein.Accessions":"Q9UGP8","Protein.Descriptions":"Translocation protein SEC63 homolog OS=Homo sapiens GN=SEC63 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":788.10645,"MH...Da.":2362.30478,"Theo..MH...Da.":2362.29722,"DeltaM..ppm.":3.2,"Deltam.z..Da.":0.00252,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":21.87371,"Average.Reporter.S.N":24.2,"Ion.Inject.Time..ms.":33.004,"RT..min.":198.2192,"First.Scan":100299,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_01.raw","File.ID":"F11","Abundance..126":11029.98439,"Abundance..127N":11909.3485,"Abundance..127C":12367.12192,"Abundance..128N":14388.15234,"Abundance..128C":14015.04333,"Abundance..129N":11025.47141,"Abundance..129C":14411.12798,"Abundance..130N":14679.92337,"Abundance..130C":12787.25148,"Abundance..131":13831.28335,"Ions.Score":16,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.24044,"Percolator.q.Value":0,"Percolator.PEP":5.346e-07}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (E2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].iISNAScTTNcLAPLAk.[V]","Modifications":"N-Term(TMT6plex); C7(Carbamidomethyl); C11(Carbamidomethyl); K17(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.8667,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":767.42596,"MH...Da.":2300.26334,"Theo..MH...Da.":2300.25979,"DeltaM..ppm.":1.54,"Deltam.z..Da.":0.00118,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":6.3108,"Average.Reporter.S.N":141.1,"Ion.Inject.Time..ms.":2.409,"RT..min.":149.9285,"First.Scan":73745,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_01.raw","File.ID":"F11","Abundance..126":1924741.74761,"Abundance..127N":1915551.40448,"Abundance..127C":1738634.69689,"Abundance..128N":1897660.10805,"Abundance..128C":1849096.15597,"Abundance..129N":1772078.26413,"Abundance..129C":1899285.14452,"Abundance..130N":1966532.82415,"Abundance..130C":1612362.30312,"Abundance..131":1852454.18186,"Ions.Score":45,"Identity.Strict":30,"Identity.Relaxed":23,"Expectation.Value":0.00037,"Percolator.q.Value":0,"Percolator.PEP":2.079e-09}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (E2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].iPSAVGYQPTLATDmGTMQEr.[I]","Modifications":"N-Term(TMT6plex); M15(Oxidation); R21(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.1569,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":841.08771,"MH...Da.":2521.24857,"Theo..MH...Da.":2521.25034,"DeltaM..ppm.":-0.7,"Deltam.z..Da.":-0.00059,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":59.09598,"Average.Reporter.S.N":19,"Ion.Inject.Time..ms.":39.463,"RT..min.":148.6158,"First.Scan":73008,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_01.raw","File.ID":"F11","Abundance..126":11223.06319,"Abundance..127N":10434.25641,"Abundance..127C":11885.82904,"Abundance..128N":13778.54136,"Abundance..128C":11967.00552,"Abundance..129N":12624.07836,"Abundance..129C":9728.43742,"Abundance..130N":12762.93955,"Abundance..130C":12233.03826,"Abundance..131":11224.00512,"Ions.Score":51,"Identity.Strict":25,"Identity.Relaxed":18,"Expectation.Value":0.00002,"Percolator.q.Value":0,"Percolator.PEP":7.183e-11}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (E2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[K].lVINGNPITIFQErDPSk.[I]","Modifications":"N-Term(TMT6plex); R14(Label:13C(6)15N(4)); K18(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":1,"Charge":3,"DeltaScore":0.6977,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":839.82446,"MH...Da.":2517.45884,"Theo..MH...Da.":2517.456,"DeltaM..ppm.":1.13,"Deltam.z..Da.":0.00095,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":0.90526,"Average.Reporter.S.N":50.5,"Ion.Inject.Time..ms.":0.706,"RT..min.":166.5327,"First.Scan":83212,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_01.raw","File.ID":"F11","Abundance..126":1275010.96502,"Abundance..127N":1182079.93142,"Abundance..127C":1210086.4251,"Abundance..128N":1330198.51449,"Abundance..128C":1304101.00431,"Abundance..129N":1306416.56276,"Abundance..129C":1510520.49228,"Abundance..130N":1372287.50584,"Abundance..130C":1350312.72429,"Abundance..131":1486248.49332,"Ions.Score":43,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.0004,"Percolator.q.Value":0,"Percolator.PEP":1.713e-08}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (E2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].iPSAVGYQPTLATDMGTMQEr.[I]","Modifications":"N-Term(TMT6plex); R21(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":835.75739,"MH...Da.":2505.2576,"Theo..MH...Da.":2505.25543,"DeltaM..ppm.":0.87,"Deltam.z..Da.":0.00072,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":5.62423,"Average.Reporter.S.N":23.2,"Ion.Inject.Time..ms.":2.677,"RT..min.":162.76,"First.Scan":81054,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_01.raw","File.ID":"F11","Abundance..126":144854.30666,"Abundance..127N":119264.28904,"Abundance..127C":127028.09576,"Abundance..128N":137410.16439,"Abundance..128C":133691.77244,"Abundance..129N":112496.34047,"Abundance..129C":114910.43079,"Abundance..130N":133716.01628,"Abundance..130C":140470.46937,"Abundance..131":129125.91788,"Ions.Score":74,"Identity.Strict":26,"Identity.Relaxed":19,"Expectation.Value":1.66516e-07,"Percolator.q.Value":0,"Percolator.PEP":5.345e-11}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (E2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].fGQAATMEGIGAIGGTPPAFNr.[A]","Modifications":"N-Term(TMT6plex); R22(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q15233","Master.Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","Protein.Accessions":"Q15233","Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.878,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":801.41833,"MH...Da.":2402.24045,"Theo..MH...Da.":2402.23635,"DeltaM..ppm.":1.71,"Deltam.z..Da.":0.00137,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":7.31281,"Average.Reporter.S.N":17.2,"Ion.Inject.Time..ms.":4.592,"RT..min.":170.0505,"First.Scan":85185,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_01.raw","File.ID":"F11","Abundance..126":28124.03551,"Abundance..127N":31203.98558,"Abundance..127C":31027.49132,"Abundance..128N":40178.48201,"Abundance..128C":38672.6088,"Abundance..129N":39251.0258,"Abundance..129C":31400.05331,"Abundance..130N":43054.20361,"Abundance..130C":43216.35442,"Abundance..131":45648.23895,"Ions.Score":41,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":0.00061,"Percolator.q.Value":0,"Percolator.PEP":8.608e-07}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (E2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].lFVGNLPPDITEEEMrk.[L]","Modifications":"N-Term(TMT6plex); R16(Label:13C(6)15N(4)); K17(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q15233","Master.Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","Protein.Accessions":"Q15233","Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","X..Missed.Cleavages":1,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":822.12701,"MH...Da.":2464.36649,"Theo..MH...Da.":2464.36407,"DeltaM..ppm.":0.98,"Deltam.z..Da.":0.00081,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":0,"Average.Reporter.S.N":20,"Ion.Inject.Time..ms.":14.462,"RT..min.":163.2856,"First.Scan":81357,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_01.raw","File.ID":"F11","Abundance..126":25190.29688,"Abundance..127N":31712.88567,"Abundance..127C":26685.98989,"Abundance..128N":34267.02737,"Abundance..128C":30561.23128,"Abundance..129N":31995.55024,"Abundance..129C":25894.99793,"Abundance..130N":30888.5037,"Abundance..130C":30072.31204,"Abundance..131":32130.07521,"Ions.Score":48,"Identity.Strict":30,"Identity.Relaxed":23,"Expectation.Value":0.00019,"Percolator.q.Value":0,"Percolator.PEP":2.564e-09}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (E2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].fLSQPFQVAEVFTGHMGk.[L]","Modifications":"N-Term(TMT6plex); K18(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":830.45618,"MH...Da.":2489.35398,"Theo..MH...Da.":2489.35066,"DeltaM..ppm.":1.33,"Deltam.z..Da.":0.00111,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":4.55453,"Average.Reporter.S.N":46,"Ion.Inject.Time..ms.":1.686,"RT..min.":187.5116,"First.Scan":94767,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_01.raw","File.ID":"F11","Abundance..126":505741.63692,"Abundance..127N":466795.51593,"Abundance..127C":528257.54842,"Abundance..128N":601944.77438,"Abundance..128C":567515.67779,"Abundance..129N":507486.25964,"Abundance..129C":523104.13393,"Abundance..130N":492613.84264,"Abundance..130C":562107.36645,"Abundance..131":447318.02319,"Ions.Score":59,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.00001,"Percolator.q.Value":0,"Percolator.PEP":7.687e-12}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (L2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].vALTGLTVAEYFr.[D]","Modifications":"N-Term(TMT6plex); R13(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.9216,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":560.32581,"MH...Da.":1678.96286,"Theo..MH...Da.":1678.96049,"DeltaM..ppm.":1.41,"Deltam.z..Da.":0.00079,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":14.34221,"Average.Reporter.S.N":51.7,"Ion.Inject.Time..ms.":14.732,"RT..min.":181.776,"First.Scan":93084,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_02.raw","File.ID":"F4","Abundance..126":66756.44528,"Abundance..127N":71200.63259,"Abundance..127C":61548.15436,"Abundance..128N":74332.68378,"Abundance..128C":74817.11958,"Abundance..129N":57902.14619,"Abundance..129C":70060.74424,"Abundance..130N":67147.80483,"Abundance..130C":64087.77709,"Abundance..131":64628.17426,"Ions.Score":51,"Identity.Strict":30,"Identity.Relaxed":23,"Expectation.Value":0.00008,"Percolator.q.Value":0,"Percolator.PEP":4.597e-07}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (L2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].vVDLmAHMASk.[E]","Modifications":"N-Term(TMT6plex); M5(Oxidation); K11(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.7647,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":561.98517,"MH...Da.":1683.94095,"Theo..MH...Da.":1683.94175,"DeltaM..ppm.":-0.48,"Deltam.z..Da.":-0.00027,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":40.65212,"Average.Reporter.S.N":43.8,"Ion.Inject.Time..ms.":11.333,"RT..min.":105.5204,"First.Scan":50069,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_02.raw","File.ID":"F4","Abundance..126":62326.4352,"Abundance..127N":79091.89749,"Abundance..127C":88230.12684,"Abundance..128N":78145.66205,"Abundance..128C":70713.78022,"Abundance..129N":76312.7857,"Abundance..129C":81575.04092,"Abundance..130N":85379.32492,"Abundance..130C":69394.96571,"Abundance..131":56258.40553,"Ions.Score":17,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.19631,"Percolator.q.Value":0,"Percolator.PEP":0.00033}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (E2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[K].aAGASDVVLYk.[I]","Modifications":"N-Term(TMT6plex); K11(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q9NSD9","Master.Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","Protein.Accessions":"Q9NSD9","Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":0.8966,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":780.46918,"MH...Da.":1559.93108,"Theo..MH...Da.":1559.92886,"DeltaM..ppm.":1.42,"Deltam.z..Da.":0.00111,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":50.9834,"Average.Reporter.S.N":28.2,"Ion.Inject.Time..ms.":23.455,"RT..min.":121.9307,"First.Scan":58171,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_01.raw","File.ID":"F11","Abundance..126":23398.14106,"Abundance..127N":17754.90811,"Abundance..127C":22387.63006,"Abundance..128N":20048.56809,"Abundance..128C":19640.58811,"Abundance..129N":15901.57161,"Abundance..129C":19188.1319,"Abundance..130N":19437.76492,"Abundance..130C":17694.51439,"Abundance..131":17068.64771,"Ions.Score":58,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.00001,"Percolator.q.Value":0,"Percolator.PEP":1.212e-07}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (L2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].yLAEVATGDDk.[K]","Modifications":"N-Term(TMT6plex); K11(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P31947","Master.Protein.Descriptions":"14-3-3 protein sigma OS=Homo sapiens GN=SFN PE=1 SV=1","Protein.Accessions":"P31947","Protein.Descriptions":"14-3-3 protein sigma OS=Homo sapiens GN=SFN PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":0.8625,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":824.45953,"MH...Da.":1647.91179,"Theo..MH...Da.":1647.90852,"DeltaM..ppm.":1.98,"Deltam.z..Da.":0.00163,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":0,"Average.Reporter.S.N":67.3,"Ion.Inject.Time..ms.":19.233,"RT..min.":126.116,"First.Scan":61584,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_02.raw","File.ID":"F4","Abundance..126":31291.72349,"Abundance..127N":31639.94896,"Abundance..127C":30964.46689,"Abundance..128N":25185.51629,"Abundance..128C":34980.92683,"Abundance..129N":32690.88323,"Abundance..129C":37051.1591,"Abundance..130N":32373.10794,"Abundance..130C":30030.0917,"Abundance..131":30793.13434,"Ions.Score":80,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":6.83828e-08,"Percolator.q.Value":0,"Percolator.PEP":3.067e-08}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (E2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].tIAMDGTEGLVr.[G]","Modifications":"N-Term(TMT6plex); R12(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":0.9333,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":751.4104,"MH...Da.":1501.81352,"Theo..MH...Da.":1501.81211,"DeltaM..ppm.":0.94,"Deltam.z..Da.":0.00071,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":45.90305,"Average.Reporter.S.N":29.2,"Ion.Inject.Time..ms.":12.195,"RT..min.":126.6766,"First.Scan":60768,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_01.raw","File.ID":"F11","Abundance..126":43459.52271,"Abundance..127N":37521.34908,"Abundance..127C":48244.45732,"Abundance..128N":33657.13117,"Abundance..128C":38475.04937,"Abundance..129N":39940.51295,"Abundance..129C":43096.6518,"Abundance..130N":37161.85862,"Abundance..130C":27884.37749,"Abundance..131":37176.0082,"Ions.Score":60,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":7.6941e-06,"Percolator.q.Value":0,"Percolator.PEP":1.126e-07}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (H2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].aAGASDVVLYk.[I]","Modifications":"N-Term(TMT6plex); K11(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q9NSD9","Master.Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","Protein.Accessions":"Q9NSD9","Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":0.9286,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":780.46924,"MH...Da.":1559.9312,"Theo..MH...Da.":1559.92886,"DeltaM..ppm.":1.5,"Deltam.z..Da.":0.00117,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":29.02768,"Average.Reporter.S.N":24.4,"Ion.Inject.Time..ms.":12.971,"RT..min.":121.4143,"First.Scan":58715,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_02.raw","File.ID":"F8","Abundance..126":29445.97852,"Abundance..127N":29113.2085,"Abundance..127C":31052.64773,"Abundance..128N":30906.04102,"Abundance..128C":31222.14275,"Abundance..129N":26182.85494,"Abundance..129C":32803.51638,"Abundance..130N":23148.58933,"Abundance..130C":28985.68026,"Abundance..131":30605.33925,"Ions.Score":56,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.00002,"Percolator.q.Value":0,"Percolator.PEP":8.91e-06}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (I2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].vIISAPSADAPMFVmGVNHEk.[Y]","Modifications":"N-Term(TMT6plex); M15(Oxidation); K21(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.4444,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":899.15417,"MH...Da.":2695.44797,"Theo..MH...Da.":2695.4443,"DeltaM..ppm.":1.36,"Deltam.z..Da.":0.00122,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":7.86863,"Average.Reporter.S.N":18.3,"Ion.Inject.Time..ms.":5.857,"RT..min.":152.933,"First.Scan":81612,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_01.raw","File.ID":"F7","Abundance..126":41171.11446,"Abundance..127N":32771.04769,"Abundance..127C":39960.32136,"Abundance..128N":34923.53946,"Abundance..128C":30838.51438,"Abundance..129N":37363.41269,"Abundance..129C":45262.98713,"Abundance..130N":28996.86425,"Abundance..130C":35865.92982,"Abundance..131":28481.11976,"Ions.Score":36,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.00219,"Percolator.q.Value":0,"Percolator.PEP":0.00005}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (H2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[R].aAPGAEFAPNk.[R]","Modifications":"N-Term(TMT6plex); K11(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q15233","Master.Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","Protein.Accessions":"Q15233","Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":0.8621,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":769.94531,"MH...Da.":1538.88335,"Theo..MH...Da.":1538.88225,"DeltaM..ppm.":0.72,"Deltam.z..Da.":0.00055,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":27.2511,"Average.Reporter.S.N":32.3,"Ion.Inject.Time..ms.":9.761,"RT..min.":99.8252,"First.Scan":46762,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_02.raw","File.ID":"F8","Abundance..126":21519.70682,"Abundance..127N":25276.36219,"Abundance..127C":22149.38694,"Abundance..128N":24101.54865,"Abundance..128C":28225.79677,"Abundance..129N":24471.42341,"Abundance..129C":25126.72013,"Abundance..130N":22804.55206,"Abundance..130C":20403.77386,"Abundance..131":20448.88593,"Ions.Score":58,"Identity.Strict":30,"Identity.Relaxed":23,"Expectation.Value":0.00002,"Percolator.q.Value":0,"Percolator.PEP":1.653e-06}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (I2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].vIISAPSADAPMFVMGVNHEk.[Y]","Modifications":"N-Term(TMT6plex); K21(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":893.82208,"MH...Da.":2679.45169,"Theo..MH...Da.":2679.44938,"DeltaM..ppm.":0.86,"Deltam.z..Da.":0.00077,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":6.08636,"Average.Reporter.S.N":47.9,"Ion.Inject.Time..ms.":2.706,"RT..min.":164.2538,"First.Scan":88354,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_01.raw","File.ID":"F7","Abundance..126":867896.96543,"Abundance..127N":744478.32181,"Abundance..127C":696231.94141,"Abundance..128N":767927.3051,"Abundance..128C":813019.71656,"Abundance..129N":709866.72148,"Abundance..129C":712871.68489,"Abundance..130N":825655.47226,"Abundance..130C":783042.64933,"Abundance..131":636896.60594,"Ions.Score":83,"Identity.Strict":30,"Identity.Relaxed":23,"Expectation.Value":4.61659e-08,"Percolator.q.Value":0,"Percolator.PEP":2.043e-09}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (H2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].rDLLFQALGr.[T]","Modifications":"N-Term(TMT6plex); R1(Label:13C(6)15N(4)); R10(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q9NSD9","Master.Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","Protein.Accessions":"Q9NSD9","Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","X..Missed.Cleavages":1,"Charge":3,"DeltaScore":0.9808,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":479.95969,"MH...Da.":1437.86451,"Theo..MH...Da.":1437.86424,"DeltaM..ppm.":0.19,"Deltam.z..Da.":0.00009,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":9.58246,"Average.Reporter.S.N":24.5,"Ion.Inject.Time..ms.":19.482,"RT..min.":136.2628,"First.Scan":66991,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_02.raw","File.ID":"F8","Abundance..126":15262.30013,"Abundance..127N":12982.71948,"Abundance..127C":13124.25251,"Abundance..128N":14136.70233,"Abundance..128C":10764.84706,"Abundance..129N":12911.31352,"Abundance..129C":12220.79765,"Abundance..130N":11381.65929,"Abundance..130C":11945.16549,"Abundance..131":13948.54236,"Ions.Score":52,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.00005,"Percolator.q.Value":0,"Percolator.PEP":8.307e-06}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (H2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].lVEALcAAGHr.[A]","Modifications":"N-Term(TMT6plex); C6(Carbamidomethyl); R11(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P23919","Master.Protein.Descriptions":"Thymidylate kinase OS=Homo sapiens GN=DTYMK PE=1 SV=4","Protein.Accessions":"P23919","Protein.Descriptions":"Thymidylate kinase OS=Homo sapiens GN=DTYMK PE=1 SV=4","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":479.26871,"MH...Da.":1435.79157,"Theo..MH...Da.":1435.79165,"DeltaM..ppm.":-0.06,"Deltam.z..Da.":-0.00003,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":10.65764,"Average.Reporter.S.N":30.1,"Ion.Inject.Time..ms.":3.204,"RT..min.":103.6921,"First.Scan":48854,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_02.raw","File.ID":"F8","Abundance..126":55139.08576,"Abundance..127N":50105.60705,"Abundance..127C":40943.03731,"Abundance..128N":48182.12573,"Abundance..128C":53924.73703,"Abundance..129N":45330.45623,"Abundance..129C":53877.04062,"Abundance..130N":46516.26274,"Abundance..130C":53489.50206,"Abundance..131":53015.8679,"Ions.Score":38,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":0.00123,"Percolator.q.Value":0,"Percolator.PEP":0.00002}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (H2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].iGLFGGAGVGk.[T]","Modifications":"N-Term(TMT6plex); K11(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":0.8966,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":721.45551,"MH...Da.":1441.90373,"Theo..MH...Da.":1441.90226,"DeltaM..ppm.":1.03,"Deltam.z..Da.":0.00074,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":30.76268,"Average.Reporter.S.N":68,"Ion.Inject.Time..ms.":2.103,"RT..min.":152.5148,"First.Scan":76231,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_02.raw","File.ID":"F8","Abundance..126":373552.81564,"Abundance..127N":362638.52754,"Abundance..127C":423119.91588,"Abundance..128N":386874.79743,"Abundance..128C":439790.1609,"Abundance..129N":348213.74179,"Abundance..129C":338338.36159,"Abundance..130N":362899.56796,"Abundance..130C":361635.66194,"Abundance..131":355439.87138,"Ions.Score":58,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":0.00001,"Percolator.q.Value":0,"Percolator.PEP":9.703e-06}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (I2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].dQEGQDVLLFIDNIFr.[F]","Modifications":"N-Term(TMT6plex); R16(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":1081.0719,"MH...Da.":2161.13652,"Theo..MH...Da.":2161.13662,"DeltaM..ppm.":-0.04,"Deltam.z..Da.":-0.00005,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":15.61625,"Average.Reporter.S.N":24.8,"Ion.Inject.Time..ms.":25.441,"RT..min.":232.3514,"First.Scan":126425,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_01.raw","File.ID":"F7","Abundance..126":19213.14366,"Abundance..127N":19247.6219,"Abundance..127C":20829.20189,"Abundance..128N":18976.04398,"Abundance..128C":21093.6554,"Abundance..129N":20638.63731,"Abundance..129C":22385.5787,"Abundance..130N":21841.1984,"Abundance..130C":21962.04249,"Abundance..131":23280.22335,"Ions.Score":55,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":0.00002,"Percolator.q.Value":0,"Percolator.PEP":1.884e-07}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (I2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].dYEFMWNPHLGYILTcPSNLGTGLr.[A]","Modifications":"N-Term(TMT6plex); C16(Carbamidomethyl); R25(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P12277","Master.Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","Protein.Accessions":"P12277","Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":1065.19336,"MH...Da.":3193.56552,"Theo..MH...Da.":3193.5676,"DeltaM..ppm.":-0.65,"Deltam.z..Da.":-0.00069,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":100,"Average.Reporter.S.N":18.4,"Ion.Inject.Time..ms.":9.391,"RT..min.":190.1464,"First.Scan":103529,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_01.raw","File.ID":"F7","Abundance..126":21638.93693,"Abundance..127N":16500.88764,"Abundance..127C":19843.36887,"Abundance..128N":21886.18841,"Abundance..128C":23310.93474,"Abundance..129N":24024.32168,"Abundance..129C":24482.13098,"Abundance..130N":16404.45782,"Abundance..130C":17322.65333,"Abundance..131":22173.78781,"Ions.Score":37,"Identity.Strict":26,"Identity.Relaxed":19,"Expectation.Value":0.00097,"Percolator.q.Value":0,"Percolator.PEP":0.00004}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (H2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].fEDYLNAESr.[V]","Modifications":"N-Term(TMT6plex); R10(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q16181","Master.Protein.Descriptions":"Septin-7 OS=Homo sapiens GN=SEPT7 PE=1 SV=2","Protein.Accessions":"Q16181","Protein.Descriptions":"Septin-7 OS=Homo sapiens GN=SEPT7 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":741.87036,"MH...Da.":1482.73345,"Theo..MH...Da.":1482.73016,"DeltaM..ppm.":2.22,"Deltam.z..Da.":0.00164,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":11.00991,"Average.Reporter.S.N":29.2,"Ion.Inject.Time..ms.":12,"RT..min.":136.7446,"First.Scan":67260,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_02.raw","File.ID":"F8","Abundance..126":23033.93648,"Abundance..127N":22970.10703,"Abundance..127C":18971.13458,"Abundance..128N":25113.05371,"Abundance..128C":21965.44989,"Abundance..129N":23732.45268,"Abundance..129C":26456.51396,"Abundance..130N":22408.40857,"Abundance..130C":24512.09589,"Abundance..131":22779.61707,"Ions.Score":45,"Identity.Strict":22,"Identity.Relaxed":15,"Expectation.Value":0.00006,"Percolator.q.Value":0,"Percolator.PEP":0.00012}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (I2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[R].cSEGSFLLTTFPrPVTVEPMDQLDDEEGLPEk.[L]","Modifications":"N-Term(TMT6plex); C1(Carbamidomethyl); R13(Label:13C(6)15N(4)); K32(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q15233","Master.Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","Protein.Accessions":"Q15233","Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","X..Missed.Cleavages":1,"Charge":4,"DeltaScore":0.8148,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":1029.02136,"MH...Da.":4113.06362,"Theo..MH...Da.":4113.05674,"DeltaM..ppm.":1.67,"Deltam.z..Da.":0.00172,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":2.66523,"Average.Reporter.S.N":29.2,"Ion.Inject.Time..ms.":2.052,"RT..min.":187.383,"First.Scan":101929,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_01.raw","File.ID":"F7","Abundance..126":189152.7441,"Abundance..127N":205292.11601,"Abundance..127C":195222.02597,"Abundance..128N":167848.30129,"Abundance..128C":186891.9816,"Abundance..129N":171667.41201,"Abundance..129C":203345.7212,"Abundance..130N":192070.4865,"Abundance..130C":211021.35256,"Abundance..131":200919.68002,"Ions.Score":27,"Identity.Strict":26,"Identity.Relaxed":19,"Expectation.Value":0.00873,"Percolator.q.Value":0,"Percolator.PEP":0.00009}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (H2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].yAFSGVAFTGAk.[E]","Modifications":"N-Term(TMT6plex); K12(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P23919","Master.Protein.Descriptions":"Thymidylate kinase OS=Homo sapiens GN=DTYMK PE=1 SV=4","Protein.Accessions":"P23919","Protein.Descriptions":"Thymidylate kinase OS=Homo sapiens GN=DTYMK PE=1 SV=4","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":0.9808,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":842.9826,"MH...Da.":1684.95793,"Theo..MH...Da.":1684.95541,"DeltaM..ppm.":1.5,"Deltam.z..Da.":0.00126,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":20.32278,"Average.Reporter.S.N":22.5,"Ion.Inject.Time..ms.":14.262,"RT..min.":152.3367,"First.Scan":76131,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_02.raw","File.ID":"F8","Abundance..126":31243.47524,"Abundance..127N":34920.15649,"Abundance..127C":29766.45857,"Abundance..128N":37412.22116,"Abundance..128C":40796.48536,"Abundance..129N":38766.65361,"Abundance..129C":36828.06343,"Abundance..130N":34856.16463,"Abundance..130C":37690.43242,"Abundance..131":37651.83761,"Ions.Score":52,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.00006,"Percolator.q.Value":0,"Percolator.PEP":1.709e-06}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (I2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].gTGGVDTAAVGGVFDVSNADr.[L]","Modifications":"N-Term(TMT6plex); R21(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P12277","Master.Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","Protein.Accessions":"P12277","Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":735.37195,"MH...Da.":2204.10129,"Theo..MH...Da.":2204.10202,"DeltaM..ppm.":-0.33,"Deltam.z..Da.":-0.00024,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":32.0199,"Average.Reporter.S.N":16.9,"Ion.Inject.Time..ms.":4.998,"RT..min.":148.23,"First.Scan":78799,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_01.raw","File.ID":"F7","Abundance..126":30507.75213,"Abundance..127N":25280.80476,"Abundance..127C":34797.70048,"Abundance..128N":30093.80921,"Abundance..128C":31532.46809,"Abundance..129N":28760.25611,"Abundance..129C":32915.74557,"Abundance..130N":29458.29648,"Abundance..130C":28003.25922,"Abundance..131":37005.4368,"Ions.Score":39,"Identity.Strict":25,"Identity.Relaxed":18,"Expectation.Value":0.00048,"Percolator.q.Value":0,"Percolator.PEP":7.945e-07}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (H2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[R].vVDLMAHMASk.[E]","Modifications":"N-Term(TMT6plex); K11(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.8409,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":556.65405,"MH...Da.":1667.9476,"Theo..MH...Da.":1667.94684,"DeltaM..ppm.":0.46,"Deltam.z..Da.":0.00026,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":32.31328,"Average.Reporter.S.N":60.7,"Ion.Inject.Time..ms.":10.821,"RT..min.":146.6065,"First.Scan":72852,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_02.raw","File.ID":"F8","Abundance..126":168487.87379,"Abundance..127N":176572.68074,"Abundance..127C":192249.48654,"Abundance..128N":213341.23897,"Abundance..128C":179930.61222,"Abundance..129N":161598.43969,"Abundance..129C":210893.37414,"Abundance..130N":195566.50437,"Abundance..130C":174228.90969,"Abundance..131":174789.60984,"Ions.Score":44,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.00038,"Percolator.q.Value":0,"Percolator.PEP":6.926e-08}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (I2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].iYEFPETDDEEENk.[L]","Modifications":"N-Term(TMT6plex); K14(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q16181","Master.Protein.Descriptions":"Septin-7 OS=Homo sapiens GN=SEPT7 PE=1 SV=2","Protein.Accessions":"Q16181","Protein.Descriptions":"Septin-7 OS=Homo sapiens GN=SEPT7 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":1112.54675,"MH...Da.":2224.08623,"Theo..MH...Da.":2224.07889,"DeltaM..ppm.":3.3,"Deltam.z..Da.":0.00367,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":100,"Average.Reporter.S.N":26.7,"Ion.Inject.Time..ms.":11.983,"RT..min.":146.3343,"First.Scan":77665,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_01.raw","File.ID":"F7","Abundance..126":9693.88698,"Abundance..127N":9304.11358,"Abundance..127C":9177.30435,"Abundance..128N":12530.47211,"Abundance..128C":12563.92914,"Abundance..129N":11413.08276,"Abundance..129C":13375.95484,"Abundance..130N":12412.48925,"Abundance..130C":11471.67811,"Abundance..131":8933.53498,"Ions.Score":20,"Identity.Strict":24,"Identity.Relaxed":17,"Expectation.Value":0.03314,"Percolator.q.Value":0,"Percolator.PEP":0.00006}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (H2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].fTQAGSEVSALLGr.[I]","Modifications":"N-Term(TMT6plex); R14(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.875,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":558.98029,"MH...Da.":1674.9263,"Theo..MH...Da.":1674.92517,"DeltaM..ppm.":0.68,"Deltam.z..Da.":0.00038,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":11.76052,"Average.Reporter.S.N":28.1,"Ion.Inject.Time..ms.":8.093,"RT..min.":162.3243,"First.Scan":81893,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_02.raw","File.ID":"F8","Abundance..126":179104.26345,"Abundance..127N":166879.19546,"Abundance..127C":142452.17958,"Abundance..128N":153428.97086,"Abundance..128C":141441.0938,"Abundance..129N":150066.50319,"Abundance..129C":169981.06245,"Abundance..130N":131774.28863,"Abundance..130C":167830.71081,"Abundance..131":184578.84747,"Ions.Score":80,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":9.54191e-08,"Percolator.q.Value":0,"Percolator.PEP":1.418e-09}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (I2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[R].aIAELGIYPAVDPLDSTSr.[I]","Modifications":"N-Term(TMT6plex); R19(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":743.07397,"MH...Da.":2227.20737,"Theo..MH...Da.":2227.2047,"DeltaM..ppm.":1.2,"Deltam.z..Da.":0.00089,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":7.02227,"Average.Reporter.S.N":47.7,"Ion.Inject.Time..ms.":3.378,"RT..min.":184.2682,"First.Scan":100104,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_01.raw","File.ID":"F7","Abundance..126":292718.2883,"Abundance..127N":314395.71997,"Abundance..127C":326803.79799,"Abundance..128N":332523.33585,"Abundance..128C":355447.37892,"Abundance..129N":320686.52087,"Abundance..129C":338991.59692,"Abundance..130N":278042.77163,"Abundance..130C":301142.98163,"Abundance..131":278001.69393,"Ions.Score":37,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.00174,"Percolator.q.Value":0,"Percolator.PEP":0.00004}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (H2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[R].vALTGLTVAEYFr.[D]","Modifications":"N-Term(TMT6plex); R13(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":0.9872,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":839.98492,"MH...Da.":1678.96257,"Theo..MH...Da.":1678.96049,"DeltaM..ppm.":1.24,"Deltam.z..Da.":0.00104,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":38.52262,"Average.Reporter.S.N":14.8,"Ion.Inject.Time..ms.":3.065,"RT..min.":181.9052,"First.Scan":92973,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_02.raw","File.ID":"F8","Abundance..126":99479.65884,"Abundance..127N":107119.64501,"Abundance..127C":84692.03993,"Abundance..128N":115622.30911,"Abundance..128C":139968.72335,"Abundance..129N":106712.65288,"Abundance..129C":104361.96729,"Abundance..130N":104362.36773,"Abundance..130C":104831.98359,"Abundance..131":113061.41642,"Ions.Score":78,"Identity.Strict":30,"Identity.Relaxed":23,"Expectation.Value":1.69411e-07,"Percolator.q.Value":0,"Percolator.PEP":4.473e-07}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (I2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[R].lFVGNLPPDITEEEMr.[K]","Modifications":"N-Term(TMT6plex); R16(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q15233","Master.Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","Protein.Accessions":"Q15233","Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":1050.0509,"MH...Da.":2099.09453,"Theo..MH...Da.":2099.09198,"DeltaM..ppm.":1.22,"Deltam.z..Da.":0.00128,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":41.9379,"Average.Reporter.S.N":10.9,"Ion.Inject.Time..ms.":30.405,"RT..min.":176.511,"First.Scan":95576,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_01.raw","File.ID":"F7","Abundance..126":10820.89189,"Abundance..127N":8909.62586,"Abundance..127C":12891.50119,"Abundance..128N":11524.86881,"Abundance..128C":6561.78757,"Abundance..129N":11744.39365,"Abundance..129C":12767.54414,"Abundance..130N":9120.76346,"Abundance..130C":9989.10898,"Abundance..131":11116.06382,"Ions.Score":73,"Identity.Strict":27,"Identity.Relaxed":20,"Expectation.Value":3.34497e-07,"Percolator.q.Value":0,"Percolator.PEP":1.344e-06}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (I2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].sAYQEAMDISkk.[E]","Modifications":"N-Term(TMT6plex); K11(Label); K12(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P31947","Master.Protein.Descriptions":"14-3-3 protein sigma OS=Homo sapiens GN=SFN PE=1 SV=1","Protein.Accessions":"P31947","Protein.Descriptions":"14-3-3 protein sigma OS=Homo sapiens GN=SFN PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":692.06561,"MH...Da.":2074.18229,"Theo..MH...Da.":2074.17924,"DeltaM..ppm.":1.47,"Deltam.z..Da.":0.00102,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":34.87837,"Average.Reporter.S.N":17.1,"Ion.Inject.Time..ms.":3.366,"RT..min.":113.5966,"First.Scan":58123,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_01.raw","File.ID":"F7","Abundance..126":98235.9151,"Abundance..127N":83863.94058,"Abundance..127C":96073.4809,"Abundance..128N":96423.26538,"Abundance..128C":113683.54849,"Abundance..129N":129410.24091,"Abundance..129C":109622.14533,"Abundance..130N":104155.82936,"Abundance..130C":108479.47688,"Abundance..131":107632.3007,"Ions.Score":17,"Identity.Strict":30,"Identity.Relaxed":23,"Expectation.Value":0.2025,"Percolator.q.Value":0,"Percolator.PEP":0.00002}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (H2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].nPGFEIIHGLLDr.[I]","Modifications":"N-Term(TMT6plex); R13(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q9NSD9","Master.Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","Protein.Accessions":"Q9NSD9","Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.9831,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":573.99255,"MH...Da.":1719.96311,"Theo..MH...Da.":1719.96189,"DeltaM..ppm.":0.71,"Deltam.z..Da.":0.00041,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":11.14581,"Average.Reporter.S.N":36,"Ion.Inject.Time..ms.":10.88,"RT..min.":165.6718,"First.Scan":83814,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_02.raw","File.ID":"F8","Abundance..126":79652.49626,"Abundance..127N":94203.38921,"Abundance..127C":94607.19765,"Abundance..128N":91211.93257,"Abundance..128C":115338.35865,"Abundance..129N":109246.69679,"Abundance..129C":97250.8194,"Abundance..130N":96591.63015,"Abundance..130C":87172.52715,"Abundance..131":100811.41292,"Ions.Score":59,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.00001,"Percolator.q.Value":0,"Percolator.PEP":4.073e-07}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (I2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].aDTLTPEEcQQFkk.[Q]","Modifications":"N-Term(TMT6plex); C9(Carbamidomethyl); K13(Label); K14(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q16181","Master.Protein.Descriptions":"Septin-7 OS=Homo sapiens GN=SEPT7 PE=1 SV=2","Protein.Accessions":"Q16181","Protein.Descriptions":"Septin-7 OS=Homo sapiens GN=SEPT7 PE=1 SV=2","X..Missed.Cleavages":1,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":800.11102,"MH...Da.":2398.31852,"Theo..MH...Da.":2398.32261,"DeltaM..ppm.":-1.71,"Deltam.z..Da.":-0.00136,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":77.63197,"Average.Reporter.S.N":14.4,"Ion.Inject.Time..ms.":10.73,"RT..min.":108.6716,"First.Scan":55163,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_01.raw","File.ID":"F7","Abundance..126":28321.24275,"Abundance..127N":20435.40083,"Abundance..127C":27254.18793,"Abundance..128N":32210.87116,"Abundance..128C":28829.74547,"Abundance..129N":30546.13833,"Abundance..129C":27368.42633,"Abundance..130N":27124.72839,"Abundance..130C":30564.52648,"Abundance..131":23143.7559,"Ions.Score":15,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.32268,"Percolator.q.Value":0,"Percolator.PEP":0.00013}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (I2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].iPSAVGYQPTLATDMGTMQEr.[I]","Modifications":"N-Term(TMT6plex); R21(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.9839,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":835.75757,"MH...Da.":2505.25815,"Theo..MH...Da.":2505.25543,"DeltaM..ppm.":1.09,"Deltam.z..Da.":0.00091,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":25.02328,"Average.Reporter.S.N":12.1,"Ion.Inject.Time..ms.":2.396,"RT..min.":160.8374,"First.Scan":86323,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_01.raw","File.ID":"F7","Abundance..126":59659.15887,"Abundance..127N":53308.90485,"Abundance..127C":75373.31976,"Abundance..128N":76830.37583,"Abundance..128C":66306.99871,"Abundance..129N":50329.37068,"Abundance..129C":65880.69285,"Abundance..130N":63740.47242,"Abundance..130C":41075.8,"Abundance..131":57574.42121,"Ions.Score":62,"Identity.Strict":26,"Identity.Relaxed":19,"Expectation.Value":2.73684e-06,"Percolator.q.Value":0,"Percolator.PEP":1.251e-06}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (I2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].fLSQPFQVAEVFTGHmGk.[L]","Modifications":"N-Term(TMT6plex); M16(Oxidation); K18(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.9762,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":835.78766,"MH...Da.":2505.34842,"Theo..MH...Da.":2505.34557,"DeltaM..ppm.":1.14,"Deltam.z..Da.":0.00095,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":13.13613,"Average.Reporter.S.N":12.7,"Ion.Inject.Time..ms.":2.241,"RT..min.":175.4854,"First.Scan":94974,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_01.raw","File.ID":"F7","Abundance..126":36499.45646,"Abundance..127N":36364.89426,"Abundance..127C":52238.54214,"Abundance..128N":66758.09749,"Abundance..128C":53310.79752,"Abundance..129N":59983.34012,"Abundance..129C":54050.46943,"Abundance..130N":66366.46543,"Abundance..130C":48351.54753,"Abundance..131":52268.63075,"Ions.Score":42,"Identity.Strict":30,"Identity.Relaxed":23,"Expectation.Value":0.00061,"Percolator.q.Value":0,"Percolator.PEP":1.025e-06}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (I2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].iPSAVGYQPTLATDmGTMQEr.[I]","Modifications":"N-Term(TMT6plex); M15(Oxidation); R21(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.2321,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":841.08954,"MH...Da.":2521.25406,"Theo..MH...Da.":2521.25034,"DeltaM..ppm.":1.47,"Deltam.z..Da.":0.00124,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":44.40543,"Average.Reporter.S.N":8.5,"Ion.Inject.Time..ms.":4.845,"RT..min.":147.3851,"First.Scan":78290,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_01.raw","File.ID":"F7","Abundance..126":52477.71137,"Abundance..127N":30858.10684,"Abundance..127C":25408.1023,"Abundance..128N":40967.5091,"Abundance..128C":46111.87007,"Abundance..129N":32694.75036,"Abundance..129C":37982.97108,"Abundance..130N":20753.92598,"Abundance..130C":29721.08134,"Abundance..131":31358.27451,"Ions.Score":56,"Identity.Strict":25,"Identity.Relaxed":18,"Expectation.Value":0.00001,"Percolator.q.Value":0,"Percolator.PEP":6.114e-07}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (I2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].iPSAVGYQPTLATDMGTmQEr.[I]","Modifications":"N-Term(TMT6plex); M18(Oxidation); R21(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.3548,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":841.08789,"MH...Da.":2521.24912,"Theo..MH...Da.":2521.25034,"DeltaM..ppm.":-0.49,"Deltam.z..Da.":-0.00041,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":44.6888,"Average.Reporter.S.N":8.9,"Ion.Inject.Time..ms.":7.852,"RT..min.":152.8332,"First.Scan":81551,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_01.raw","File.ID":"F7","Abundance..126":9082.01402,"Abundance..127N":13190.7636,"Abundance..127C":10897.88983,"Abundance..128N":10969.26346,"Abundance..128C":10462.63139,"Abundance..129N":12941.00469,"Abundance..129C":11765.49594,"Abundance..130N":10265.26004,"Abundance..130C":12106.50116,"Abundance..131":14476.04204,"Ions.Score":62,"Identity.Strict":25,"Identity.Relaxed":18,"Expectation.Value":1.87133e-06,"Percolator.q.Value":0,"Percolator.PEP":1.28e-07}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (H2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].sPLAQMEEErr.[E]","Modifications":"N-Term(TMT6plex); R10(Label:13C(6)15N(4)); R11(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q16181","Master.Protein.Descriptions":"Septin-7 OS=Homo sapiens GN=SEPT7 PE=1 SV=2","Protein.Accessions":"Q16181","Protein.Descriptions":"Septin-7 OS=Homo sapiens GN=SEPT7 PE=1 SV=2","X..Missed.Cleavages":1,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":532.28247,"MH...Da.":1594.83286,"Theo..MH...Da.":1594.83234,"DeltaM..ppm.":0.32,"Deltam.z..Da.":0.00017,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":9.69578,"Average.Reporter.S.N":7.7,"Ion.Inject.Time..ms.":3.228,"RT..min.":74.8841,"First.Scan":33201,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_02.raw","File.ID":"F8","Abundance..126":29540.77759,"Abundance..127N":35892.52782,"Abundance..127C":31706.95407,"Abundance..128N":23982.74093,"Abundance..128C":30536.4309,"Abundance..129N":22661.90168,"Abundance..129C":32324.29233,"Abundance..130N":25246.50462,"Abundance..130C":30429.25572,"Abundance..131":23758.38423,"Ions.Score":36,"Identity.Strict":27,"Identity.Relaxed":20,"Expectation.Value":0.00133,"Percolator.q.Value":0,"Percolator.PEP":0.00002}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (I2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[K].drLPLAVVGSNTIIEVNGk.[R]","Modifications":"N-Term(TMT6plex); R2(Label:13C(6)15N(4)); K19(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q16181","Master.Protein.Descriptions":"Septin-7 OS=Homo sapiens GN=SEPT7 PE=1 SV=2","Protein.Accessions":"Q16181","Protein.Descriptions":"Septin-7 OS=Homo sapiens GN=SEPT7 PE=1 SV=2","X..Missed.Cleavages":1,"Charge":3,"DeltaScore":0.9242,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":824.49603,"MH...Da.":2471.47354,"Theo..MH...Da.":2471.47165,"DeltaM..ppm.":0.77,"Deltam.z..Da.":0.00063,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":6.22924,"Average.Reporter.S.N":34.8,"Ion.Inject.Time..ms.":3.083,"RT..min.":156.7822,"First.Scan":83902,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_01.raw","File.ID":"F7","Abundance..126":127314.83931,"Abundance..127N":98353.14924,"Abundance..127C":99284.74433,"Abundance..128N":111262.98849,"Abundance..128C":126116.90264,"Abundance..129N":85113.88695,"Abundance..129C":77632.19702,"Abundance..130N":104026.25369,"Abundance..130C":100763.98327,"Abundance..131":126171.60993,"Ions.Score":66,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":1.78788e-06,"Percolator.q.Value":0,"Percolator.PEP":4.204e-07}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (I2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].fLSQPFQVAEVFTGHMGk.[L]","Modifications":"N-Term(TMT6plex); K18(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":830.45563,"MH...Da.":2489.35233,"Theo..MH...Da.":2489.35066,"DeltaM..ppm.":0.67,"Deltam.z..Da.":0.00056,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":2.16762,"Average.Reporter.S.N":12,"Ion.Inject.Time..ms.":0.567,"RT..min.":186.0054,"First.Scan":101127,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_01.raw","File.ID":"F7","Abundance..126":608400.68384,"Abundance..127N":910742.34108,"Abundance..127C":531039.70177,"Abundance..128N":769688.95295,"Abundance..128C":699521.90355,"Abundance..129N":753298.60382,"Abundance..129C":626161.2376,"Abundance..130N":919060.59746,"Abundance..130C":741426.88625,"Abundance..131":767142.34436,"Ions.Score":73,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":4.41213e-07,"Percolator.q.Value":0,"Percolator.PEP":1.601e-07}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (I2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].drLPLAVVGSNTIIEVNGk.[R]","Modifications":"N-Term(TMT6plex); R2(Label:13C(6)15N(4)); K19(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q16181","Master.Protein.Descriptions":"Septin-7 OS=Homo sapiens GN=SEPT7 PE=1 SV=2","Protein.Accessions":"Q16181","Protein.Descriptions":"Septin-7 OS=Homo sapiens GN=SEPT7 PE=1 SV=2","X..Missed.Cleavages":1,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":824.49634,"MH...Da.":2471.47446,"Theo..MH...Da.":2471.47165,"DeltaM..ppm.":1.14,"Deltam.z..Da.":0.00094,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":7.60949,"Average.Reporter.S.N":30.9,"Ion.Inject.Time..ms.":5.4,"RT..min.":156.7379,"First.Scan":83875,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_01.raw","File.ID":"F7","Abundance..126":116808.58355,"Abundance..127N":111398.20272,"Abundance..127C":117947.5813,"Abundance..128N":108326.61851,"Abundance..128C":106409.94685,"Abundance..129N":110079.00598,"Abundance..129C":102959.10591,"Abundance..130N":112309.20312,"Abundance..130C":99100.16271,"Abundance..131":101749.40373,"Ions.Score":70,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":7.78632e-07,"Percolator.q.Value":0,"Percolator.PEP":2.192e-07}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (H2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].iMNVIGEPIDEr.[G]","Modifications":"N-Term(TMT6plex); R12(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":0.967,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":812.9444,"MH...Da.":1624.88152,"Theo..MH...Da.":1624.88053,"DeltaM..ppm.":0.61,"Deltam.z..Da.":0.00049,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":12.18887,"Average.Reporter.S.N":20.5,"Ion.Inject.Time..ms.":3.085,"RT..min.":155.0414,"First.Scan":77677,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_02.raw","File.ID":"F8","Abundance..126":126722.80146,"Abundance..127N":148779.01903,"Abundance..127C":138518.72662,"Abundance..128N":115197.73119,"Abundance..128C":148566.25769,"Abundance..129N":115699.88123,"Abundance..129C":133853.05293,"Abundance..130N":140019.56249,"Abundance..130C":107343.20576,"Abundance..131":121217.0549,"Ions.Score":91,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":6.10226e-09,"Percolator.q.Value":0,"Percolator.PEP":4.971e-07}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (J2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].hEHQVmLmr.[Q]","Modifications":"N-Term(TMT6plex); M6(Oxidation); M8(Oxidation); R9(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q15233","Master.Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","Protein.Accessions":"Q15233","Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","X..Missed.Cleavages":0,"Charge":4,"DeltaScore":0.9118,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":363.68835,"MH...Da.":1451.73159,"Theo..MH...Da.":1451.73242,"DeltaM..ppm.":-0.58,"Deltam.z..Da.":-0.00021,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":14.75797,"Average.Reporter.S.N":20.1,"Ion.Inject.Time..ms.":33.474,"RT..min.":15.1738,"First.Scan":3481,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture4_03.raw","File.ID":"F6","Abundance..126":7011.03801,"Abundance..127N":7129.84845,"Abundance..127C":5562.20516,"Abundance..128N":6517.95675,"Abundance..128C":6493.44068,"Abundance..129N":6677.95324,"Abundance..129C":6695.03827,"Abundance..130N":6608.22447,"Abundance..130C":6932.21938,"Abundance..131":7071.18635,"Ions.Score":34,"Identity.Strict":24,"Identity.Relaxed":17,"Expectation.Value":0.00113,"Percolator.q.Value":0,"Percolator.PEP":0.00018}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (J2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].tTLLPGLLk.[T]","Modifications":"N-Term(TMT6plex); K9(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q9NSD9","Master.Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","Protein.Accessions":"Q9NSD9","Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":711.48309,"MH...Da.":1421.95891,"Theo..MH...Da.":1421.95871,"DeltaM..ppm.":0.14,"Deltam.z..Da.":0.0001,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":8.69568,"Average.Reporter.S.N":66,"Ion.Inject.Time..ms.":3.022,"RT..min.":164.4954,"First.Scan":86530,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture4_03.raw","File.ID":"F6","Abundance..126":281291.1515,"Abundance..127N":305236.96039,"Abundance..127C":216532.20374,"Abundance..128N":278836.19157,"Abundance..128C":319816.61769,"Abundance..129N":276682.08132,"Abundance..129C":324924.63443,"Abundance..130N":278393.0751,"Abundance..130C":257739.43938,"Abundance..131":285852.3002,"Ions.Score":44,"Identity.Strict":21,"Identity.Relaxed":14,"Expectation.Value":0.00005,"Percolator.q.Value":0,"Percolator.PEP":0.00012}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (J2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].lVEALcAAGHr.[A]","Modifications":"N-Term(TMT6plex); C6(Carbamidomethyl); R11(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P23919","Master.Protein.Descriptions":"Thymidylate kinase OS=Homo sapiens GN=DTYMK PE=1 SV=4","Protein.Accessions":"P23919","Protein.Descriptions":"Thymidylate kinase OS=Homo sapiens GN=DTYMK PE=1 SV=4","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":479.26767,"MH...Da.":1435.78846,"Theo..MH...Da.":1435.79165,"DeltaM..ppm.":-2.23,"Deltam.z..Da.":-0.00107,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":36.12501,"Average.Reporter.S.N":44.7,"Ion.Inject.Time..ms.":4.252,"RT..min.":103.8196,"First.Scan":50862,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture4_03.raw","File.ID":"F6","Abundance..126":142349.02386,"Abundance..127N":135178.71009,"Abundance..127C":134653.46148,"Abundance..128N":135758.98509,"Abundance..128C":138368.59411,"Abundance..129N":144043.30184,"Abundance..129C":141750.32756,"Abundance..130N":139413.16808,"Abundance..130C":104362.71738,"Abundance..131":124525.58363,"Ions.Score":30,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.00886,"Percolator.q.Value":0,"Percolator.PEP":0.00024}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (J2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].rDLLFQALGr.[T]","Modifications":"N-Term(TMT6plex); R1(Label:13C(6)15N(4)); R10(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q9NSD9","Master.Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","Protein.Accessions":"Q9NSD9","Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","X..Missed.Cleavages":1,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":479.95932,"MH...Da.":1437.86341,"Theo..MH...Da.":1437.86424,"DeltaM..ppm.":-0.58,"Deltam.z..Da.":-0.00028,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":58.50269,"Average.Reporter.S.N":26.8,"Ion.Inject.Time..ms.":7.488,"RT..min.":136.3127,"First.Scan":69844,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture4_03.raw","File.ID":"F6","Abundance..126":21350.40175,"Abundance..127N":29453.28869,"Abundance..127C":27146.53723,"Abundance..128N":28469.36957,"Abundance..128C":20457.04917,"Abundance..129N":30986.63351,"Abundance..129C":31614.72298,"Abundance..130N":32498.93416,"Abundance..130C":27541.58946,"Abundance..131":26014.46063,"Ions.Score":27,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.01826,"Percolator.q.Value":0,"Percolator.PEP":0.00013}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (G4)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].dQEGQDVLLFIDNIFR.[F]","Modifications":"N-Term(TMT6plex)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.9756,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":717.71564,"MH...Da.":2151.13236,"Theo..MH...Da.":2151.12835,"DeltaM..ppm.":1.86,"Deltam.z..Da.":0.00134,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":5.2928,"Average.Reporter.S.N":6,"Ion.Inject.Time..ms.":50,"RT..min.":232.8627,"First.Scan":117295,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_02.raw","File.ID":"F9","Abundance..126":1445.12455,"Abundance..127N":1545.37869,"Abundance..127C":1965.34286,"Abundance..128N":2822.31972,"Abundance..128C":1772.96578,"Abundance..129N":2813.48186,"Abundance..129C":1682.61256,"Abundance..130N":1587.3277,"Abundance..130C":2830.33716,"Abundance..131":1890.14527,"Ions.Score":41,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.00073,"Percolator.q.Value":0,"Percolator.PEP":0.00496}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (J2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].fEDYLNAESr.[V]","Modifications":"N-Term(TMT6plex); R10(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q16181","Master.Protein.Descriptions":"Septin-7 OS=Homo sapiens GN=SEPT7 PE=1 SV=2","Protein.Accessions":"Q16181","Protein.Descriptions":"Septin-7 OS=Homo sapiens GN=SEPT7 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":741.86981,"MH...Da.":1482.73235,"Theo..MH...Da.":1482.73016,"DeltaM..ppm.":1.48,"Deltam.z..Da.":0.00109,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":26.35733,"Average.Reporter.S.N":22.9,"Ion.Inject.Time..ms.":5.977,"RT..min.":136.8173,"First.Scan":70145,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture4_03.raw","File.ID":"F6","Abundance..126":61768.45051,"Abundance..127N":74959.86502,"Abundance..127C":68110.29493,"Abundance..128N":87402.24408,"Abundance..128C":70029.00507,"Abundance..129N":66393.93548,"Abundance..129C":63325.70052,"Abundance..130N":74173.10485,"Abundance..130C":75847.09565,"Abundance..131":72686.49292,"Ions.Score":50,"Identity.Strict":22,"Identity.Relaxed":15,"Expectation.Value":0.00002,"Percolator.q.Value":0,"Percolator.PEP":0.00006}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (J2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[K].lEMEMEAAr.[H]","Modifications":"N-Term(TMT6plex); R9(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q15233","Master.Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","Protein.Accessions":"Q15233","Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":0.8718,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":659.83246,"MH...Da.":1318.65764,"Theo..MH...Da.":1318.65719,"DeltaM..ppm.":0.34,"Deltam.z..Da.":0.00022,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":19.40778,"Average.Reporter.S.N":22.1,"Ion.Inject.Time..ms.":2.159,"RT..min.":113.7522,"First.Scan":56663,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture4_03.raw","File.ID":"F6","Abundance..126":156184.16079,"Abundance..127N":157066.25314,"Abundance..127C":148978.2184,"Abundance..128N":167457.84886,"Abundance..128C":191169.1502,"Abundance..129N":158802.44428,"Abundance..129C":163944.6867,"Abundance..130N":183293.03747,"Abundance..130C":148971.25574,"Abundance..131":169390.5554,"Ions.Score":39,"Identity.Strict":23,"Identity.Relaxed":16,"Expectation.Value":0.00032,"Percolator.q.Value":0,"Percolator.PEP":0.00022}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (J2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].yENGAFQEr.[A]","Modifications":"N-Term(TMT6plex); R9(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P23919","Master.Protein.Descriptions":"Thymidylate kinase OS=Homo sapiens GN=DTYMK PE=1 SV=4","Protein.Accessions":"P23919","Protein.Descriptions":"Thymidylate kinase OS=Homo sapiens GN=DTYMK PE=1 SV=4","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":0.8,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":676.83698,"MH...Da.":1352.66667,"Theo..MH...Da.":1352.66716,"DeltaM..ppm.":-0.36,"Deltam.z..Da.":-0.00025,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":18.21995,"Average.Reporter.S.N":13.3,"Ion.Inject.Time..ms.":31.27,"RT..min.":71.7722,"First.Scan":32414,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture4_03.raw","File.ID":"F6","Abundance..126":11159.76586,"Abundance..127N":13462.58937,"Abundance..127C":8498.94569,"Abundance..128N":11391.89697,"Abundance..128C":12547.67068,"Abundance..129N":8619.72044,"Abundance..129C":13098.67862,"Abundance..130N":12723.45791,"Abundance..130C":8438.33651,"Abundance..131":9084.78348,"Ions.Score":35,"Identity.Strict":20,"Identity.Relaxed":13,"Expectation.Value":0.00036,"Percolator.q.Value":0,"Percolator.PEP":0.0002}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (G2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].vETELQGVcDTVLGLLDSHLIk.[E]","Modifications":"N-Term(TMT6plex); C9(Carbamidomethyl); K22(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P31947","Master.Protein.Descriptions":"14-3-3 protein sigma OS=Homo sapiens GN=SFN PE=1 SV=1","Protein.Accessions":"P31947","Protein.Descriptions":"14-3-3 protein sigma OS=Homo sapiens GN=SFN PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":969.21222,"MH...Da.":2905.6221,"Theo..MH...Da.":2905.62001,"DeltaM..ppm.":0.72,"Deltam.z..Da.":0.0007,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":58.51701,"Average.Reporter.S.N":24.4,"Ion.Inject.Time..ms.":50,"RT..min.":220.8611,"First.Scan":111942,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_02.raw","File.ID":"F9","Abundance..126":8428.25429,"Abundance..127N":7963.9935,"Abundance..127C":8929.70883,"Abundance..128N":7969.92396,"Abundance..128C":9013.39435,"Abundance..129N":8703.75307,"Abundance..129C":9047.81243,"Abundance..130N":8924.94567,"Abundance..130C":8764.16351,"Abundance..131":8308.16581,"Ions.Score":78,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":1.47138e-07,"Percolator.q.Value":0,"Percolator.PEP":5.15e-12}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (G2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].nLPQYVSNELLEEAFSVFGQVEr.[A]","Modifications":"N-Term(TMT6plex); R23(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q15233","Master.Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","Protein.Accessions":"Q15233","Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":969.83771,"MH...Da.":2907.49857,"Theo..MH...Da.":2907.49652,"DeltaM..ppm.":0.7,"Deltam.z..Da.":0.00068,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":20.50099,"Ion.Inject.Time..ms.":50,"RT..min.":246.0002,"First.Scan":123060,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_02.raw","File.ID":"F9","Ions.Score":22,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":0.04257,"Percolator.q.Value":0,"Percolator.PEP":0.00017}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (G4)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].iISNAScTTNcLAPLAk.[V]","Modifications":"N-Term(TMT6plex); C7(Carbamidomethyl); C11(Carbamidomethyl); K17(TMT6plex)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.9492,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":764.7547,"MH...Da.":2292.24955,"Theo..MH...Da.":2292.24559,"DeltaM..ppm.":1.72,"Deltam.z..Da.":0.00132,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":54.1993,"Average.Reporter.S.N":44.6,"Ion.Inject.Time..ms.":8.257,"RT..min.":149.5004,"First.Scan":74042,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_02.raw","File.ID":"F9","Abundance..126":90367.62027,"Abundance..127N":94591.50771,"Abundance..127C":84492.13917,"Abundance..128N":87598.54069,"Abundance..128C":85846.81042,"Abundance..129N":82312.63062,"Abundance..129C":93406.82021,"Abundance..130N":75914.13272,"Abundance..130C":86561.47005,"Abundance..131":81731.9009,"Ions.Score":59,"Identity.Strict":30,"Identity.Relaxed":23,"Expectation.Value":0.00001,"Percolator.q.Value":0,"Percolator.PEP":0.00118}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (H2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].vLLLSHLAr.[M]","Modifications":"N-Term(TMT6plex); R9(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q9UGP8","Master.Protein.Descriptions":"Translocation protein SEC63 homolog OS=Homo sapiens GN=SEC63 PE=1 SV=2","Protein.Accessions":"Q9UGP8","Protein.Descriptions":"Translocation protein SEC63 homolog OS=Homo sapiens GN=SEC63 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.6786,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":420.94553,"MH...Da.":1260.82203,"Theo..MH...Da.":1260.82288,"DeltaM..ppm.":-0.68,"Deltam.z..Da.":-0.00028,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":3.98199,"Average.Reporter.S.N":29.7,"Ion.Inject.Time..ms.":39.683,"RT..min.":122.6808,"First.Scan":59400,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_02.raw","File.ID":"F8","Abundance..126":9103.70558,"Abundance..127N":9456.16754,"Abundance..127C":9640.14444,"Abundance..128N":10577.85969,"Abundance..128C":10478.03333,"Abundance..129N":9201.91242,"Abundance..129C":10357.09583,"Abundance..130N":10855.30963,"Abundance..130C":8856.2579,"Abundance..131":11759.83808,"Ions.Score":28,"Identity.Strict":23,"Identity.Relaxed":16,"Expectation.Value":0.00369,"Percolator.q.Value":0,"Percolator.PEP":0.00009}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (H2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[R].vLLLSHLAr.[M]","Modifications":"N-Term(TMT6plex); R9(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q9UGP8","Master.Protein.Descriptions":"Translocation protein SEC63 homolog OS=Homo sapiens GN=SEC63 PE=1 SV=2","Protein.Accessions":"Q9UGP8","Protein.Descriptions":"Translocation protein SEC63 homolog OS=Homo sapiens GN=SEC63 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.7391,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":420.94598,"MH...Da.":1260.8234,"Theo..MH...Da.":1260.82288,"DeltaM..ppm.":0.41,"Deltam.z..Da.":0.00017,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":25.57316,"Average.Reporter.S.N":64,"Ion.Inject.Time..ms.":24.679,"RT..min.":122.3962,"First.Scan":59248,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_02.raw","File.ID":"F8","Abundance..126":22292.22213,"Abundance..127N":19527.31654,"Abundance..127C":20421.03904,"Abundance..128N":22821.65495,"Abundance..128C":22061.81883,"Abundance..129N":21532.80351,"Abundance..129C":23030.8754,"Abundance..130N":22047.47121,"Abundance..130C":22112.88918,"Abundance..131":21169.80416,"Ions.Score":46,"Identity.Strict":23,"Identity.Relaxed":16,"Expectation.Value":0.00005,"Percolator.q.Value":0,"Percolator.PEP":8.181e-06}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (H2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[R].iLEQQNSSr.[T]","Modifications":"N-Term(TMT6plex); R9(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q16181","Master.Protein.Descriptions":"Septin-7 OS=Homo sapiens GN=SEPT7 PE=1 SV=2","Protein.Accessions":"Q16181","Protein.Descriptions":"Septin-7 OS=Homo sapiens GN=SEPT7 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":0.6327,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":657.36658,"MH...Da.":1313.72588,"Theo..MH...Da.":1313.72501,"DeltaM..ppm.":0.66,"Deltam.z..Da.":0.00043,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":0,"Average.Reporter.S.N":15.7,"Ion.Inject.Time..ms.":29.051,"RT..min.":59.9932,"First.Scan":25224,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_02.raw","File.ID":"F8","Abundance..126":5698.13728,"Abundance..127N":5402.68014,"Abundance..127C":6995.80083,"Abundance..128N":5505.13374,"Abundance..128C":6455.40204,"Abundance..129N":5406.56795,"Abundance..129C":5158.91417,"Abundance..130N":4635.37875,"Abundance..130C":5264.35268,"Abundance..131":5945.42038,"Ions.Score":49,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.00011,"Percolator.q.Value":0,"Percolator.PEP":0.00007}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (H2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[K].vELDNMPLr.[G]","Modifications":"N-Term(TMT6plex); R9(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q15233","Master.Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","Protein.Accessions":"Q15233","Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":0.8125,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":663.3703,"MH...Da.":1325.73332,"Theo..MH...Da.":1325.73241,"DeltaM..ppm.":0.69,"Deltam.z..Da.":0.00046,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":13.53003,"Average.Reporter.S.N":67.5,"Ion.Inject.Time..ms.":3.131,"RT..min.":134.3762,"First.Scan":65928,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_02.raw","File.ID":"F8","Abundance..126":196700.75674,"Abundance..127N":187253.39473,"Abundance..127C":182654.87865,"Abundance..128N":190515.0297,"Abundance..128C":174048.0877,"Abundance..129N":161653.3376,"Abundance..129C":198350.10024,"Abundance..130N":173001.14838,"Abundance..130C":162738.78098,"Abundance..131":207790.35048,"Ions.Score":48,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":0.0001,"Percolator.q.Value":0,"Percolator.PEP":0.00012}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (I2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].vDIVAINDPFIDLNYMVYMFQYDSTHGk.[F]","Modifications":"N-Term(TMT6plex); K28(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":0,"Charge":4,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":944.48169,"MH...Da.":3774.90493,"Theo..MH...Da.":3774.90431,"DeltaM..ppm.":0.16,"Deltam.z..Da.":0.00015,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":3.17599,"Average.Reporter.S.N":10.5,"Ion.Inject.Time..ms.":10.134,"RT..min.":241.0544,"First.Scan":130478,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_01.raw","File.ID":"F7","Abundance..126":4700.03994,"Abundance..127N":8579.8883,"Abundance..127C":7317.87812,"Abundance..128N":10227.10668,"Abundance..128C":8793.00063,"Abundance..129N":8172.21596,"Abundance..129C":12586.52562,"Abundance..130N":9582.13538,"Abundance..130C":9167.08621,"Abundance..131":9797.54685,"Ions.Score":47,"Identity.Strict":27,"Identity.Relaxed":20,"Expectation.Value":0.00012,"Percolator.q.Value":0,"Percolator.PEP":5.447e-07}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (H2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].gAMPPAPVPAGTPAPPGPATMMPDGTLGLTPPTTEr.[F]","Modifications":"N-Term(TMT6plex); R36(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q15233","Master.Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","Protein.Accessions":"Q15233","Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","X..Missed.Cleavages":0,"Charge":4,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":923.47498,"MH...Da.":3690.87807,"Theo..MH...Da.":3690.87704,"DeltaM..ppm.":0.28,"Deltam.z..Da.":0.00026,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":0,"Average.Reporter.S.N":4.9,"Ion.Inject.Time..ms.":5.461,"RT..min.":181.8581,"First.Scan":92946,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_02.raw","File.ID":"F8","Abundance..126":6606.10404,"Abundance..127N":8830.67311,"Abundance..127C":6195.16725,"Abundance..128N":4990.65402,"Abundance..128C":11510.18411,"Abundance..129C":8268.44599,"Abundance..130N":12869.85267,"Abundance..130C":12897.41503,"Abundance..131":8492.79755,"Ions.Score":18,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":0.12083,"Percolator.q.Value":0,"Percolator.PEP":0.00014}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (H2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[K].qFAPIHAEAPEFMEMSVEQEILVTGIk.[V]","Modifications":"N-Term(TMT6plex); K27(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.9429,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":1170.95581,"MH...Da.":3510.85288,"Theo..MH...Da.":3510.85082,"DeltaM..ppm.":0.59,"Deltam.z..Da.":0.00069,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":0,"Average.Reporter.S.N":43.8,"Ion.Inject.Time..ms.":30.781,"RT..min.":196.6137,"First.Scan":100775,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_02.raw","File.ID":"F8","Abundance..126":48269.8955,"Abundance..127N":44564.67881,"Abundance..127C":44813.97433,"Abundance..128N":55059.15068,"Abundance..128C":47664.30144,"Abundance..129N":50890.71769,"Abundance..129C":43944.84012,"Abundance..130N":40546.84168,"Abundance..130C":48070.3119,"Abundance..131":51624.89689,"Ions.Score":70,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":8.27973e-07,"Percolator.q.Value":0,"Percolator.PEP":2.366e-08}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (H2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].kGSITSVQAIYVPADDLTDPAPATTFAHLDATTVLSr.[A]","Modifications":"N-Term(TMT6plex); K1(Label); R37(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":1,"Charge":4,"DeltaScore":0.9012,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":1080.58765,"MH...Da.":4319.32876,"Theo..MH...Da.":4319.3294,"DeltaM..ppm.":-0.15,"Deltam.z..Da.":-0.00016,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":6.8563,"Average.Reporter.S.N":5.1,"Ion.Inject.Time..ms.":14.383,"RT..min.":177.6299,"First.Scan":90559,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_02.raw","File.ID":"F8","Abundance..126":27785.806,"Abundance..127N":20480.505,"Abundance..127C":30597.22494,"Abundance..128N":29352.49844,"Abundance..128C":15001.73767,"Abundance..129N":22194.53685,"Abundance..129C":22889.29725,"Abundance..130N":27834.23462,"Abundance..130C":20940.74246,"Abundance..131":20867.96983,"Ions.Score":81,"Identity.Strict":27,"Identity.Relaxed":20,"Expectation.Value":4.80569e-08,"Percolator.q.Value":0,"Percolator.PEP":2.321e-08}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (H2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].sTPSGFTLDDVIQTGVDNPGHPYIMTVGcVAGDEESYEVFk.[D]","Modifications":"N-Term(TMT6plex); C29(Carbamidomethyl); K41(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P12277","Master.Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","Protein.Accessions":"P12277","Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","X..Missed.Cleavages":0,"Charge":4,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":1225.34277,"MH...Da.":4898.34926,"Theo..MH...Da.":4898.36724,"DeltaM..ppm.":-3.67,"Deltam.z..Da.":-0.00449,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":16.1247,"Average.Reporter.S.N":14.8,"Ion.Inject.Time..ms.":50,"RT..min.":199.8233,"First.Scan":102380,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_02.raw","File.ID":"F8","Abundance..126":6767.00682,"Abundance..127N":7894.56319,"Abundance..127C":10902.38344,"Abundance..128N":10934.436,"Abundance..128C":9455.94936,"Abundance..129N":9674.76053,"Abundance..129C":10919.38425,"Abundance..130N":9656.7312,"Abundance..130C":8503.81348,"Abundance..131":9135.00011,"Ions.Score":53,"Identity.Strict":22,"Identity.Relaxed":15,"Expectation.Value":9.25729e-06,"Percolator.q.Value":0,"Percolator.PEP":7.78e-08}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (H2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].sTPSGFTLDDVIQTGVDNPGHPYIMTVGcVAGDEESYEVFk.[D]","Modifications":"N-Term(TMT6plex); C29(Carbamidomethyl); K41(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P12277","Master.Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","Protein.Accessions":"P12277","Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","X..Missed.Cleavages":0,"Charge":4,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":1225.34705,"MH...Da.":4898.36635,"Theo..MH...Da.":4898.36724,"DeltaM..ppm.":-0.18,"Deltam.z..Da.":-0.00022,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":0,"Average.Reporter.S.N":0.5,"Ion.Inject.Time..ms.":10.988,"RT..min.":198.3755,"First.Scan":101663,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_02.raw","File.ID":"F8","Abundance..126":5610.95215,"Abundance..130N":5748.19971,"Ions.Score":56,"Identity.Strict":22,"Identity.Relaxed":15,"Expectation.Value":3.8146e-06,"Percolator.q.Value":0,"Percolator.PEP":1.248e-07}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (H2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[K].gSITSVQAIYVPADDLTDPAPATTFAHLDATTVLSr.[A]","Modifications":"N-Term(TMT6plex); R36(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":1318.68945,"MH...Da.":3954.05381,"Theo..MH...Da.":3954.05731,"DeltaM..ppm.":-0.89,"Deltam.z..Da.":-0.00117,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":7.92623,"Average.Reporter.S.N":11.3,"Ion.Inject.Time..ms.":44.185,"RT..min.":190.2673,"First.Scan":97466,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_02.raw","File.ID":"F8","Abundance..126":5765.9189,"Abundance..127N":7141.96118,"Abundance..127C":8273.66706,"Abundance..128N":7359.62118,"Abundance..128C":6302.75853,"Abundance..129N":5188.32819,"Abundance..129C":4552.92387,"Abundance..130N":9314.51834,"Abundance..130C":6378.47211,"Abundance..131":6905.95527,"Ions.Score":69,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":9.60036e-07,"Percolator.q.Value":0,"Percolator.PEP":1.978e-08}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (H2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].dYEFMWNPHLGYILTcPSNLGTGLr.[A]","Modifications":"N-Term(TMT6plex); C16(Carbamidomethyl); R25(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P12277","Master.Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","Protein.Accessions":"P12277","Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":1065.19604,"MH...Da.":3193.57358,"Theo..MH...Da.":3193.5676,"DeltaM..ppm.":1.87,"Deltam.z..Da.":0.002,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":2.48772,"Average.Reporter.S.N":29.9,"Ion.Inject.Time..ms.":7.705,"RT..min.":190.3114,"First.Scan":97487,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_02.raw","File.ID":"F8","Abundance..126":99826.86416,"Abundance..127N":90589.08131,"Abundance..127C":140694.19641,"Abundance..128N":111846.88488,"Abundance..128C":110161.45642,"Abundance..129N":111607.39963,"Abundance..129C":90194.38992,"Abundance..130N":116581.52687,"Abundance..130C":117822.7853,"Abundance..131":116172.3064,"Ions.Score":78,"Identity.Strict":26,"Identity.Relaxed":19,"Expectation.Value":8.03547e-08,"Percolator.q.Value":0,"Percolator.PEP":4.379e-10}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (H2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].hDMAAAGFTEALTFALcSQEDIADk.[L]","Modifications":"N-Term(TMT6plex); C17(Carbamidomethyl); K25(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q9NSD9","Master.Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","Protein.Accessions":"Q9NSD9","Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":1060.19482,"MH...Da.":3178.56992,"Theo..MH...Da.":3178.56806,"DeltaM..ppm.":0.59,"Deltam.z..Da.":0.00062,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":9.28177,"Average.Reporter.S.N":4,"Ion.Inject.Time..ms.":21.683,"RT..min.":198.6783,"First.Scan":101824,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_02.raw","File.ID":"F8","Abundance..126":6413.45638,"Abundance..127N":3386.28444,"Abundance..127C":2757.15834,"Abundance..128N":3368.35034,"Abundance..128C":1040.22259,"Abundance..129N":4046.51858,"Abundance..129C":3321.50732,"Abundance..130N":2782.01211,"Abundance..130C":3580.93918,"Abundance..131":5504.82534,"Ions.Score":42,"Identity.Strict":25,"Identity.Relaxed":18,"Expectation.Value":0.00027,"Percolator.q.Value":0,"Percolator.PEP":0.00001}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (I2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].aHGGYSVFAGVGEr.[T]","Modifications":"N-Term(TMT6plex); R14(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":549.28882,"MH...Da.":1645.8519,"Theo..MH...Da.":1645.85234,"DeltaM..ppm.":-0.27,"Deltam.z..Da.":-0.00015,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":30.1762,"Average.Reporter.S.N":30.6,"Ion.Inject.Time..ms.":5.885,"RT..min.":86.9634,"First.Scan":42308,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_01.raw","File.ID":"F7","Abundance..126":37410.51709,"Abundance..127N":54662.08589,"Abundance..127C":55672.71209,"Abundance..128N":55688.61437,"Abundance..128C":45601.67275,"Abundance..129N":51518.25368,"Abundance..129C":46424.36125,"Abundance..130N":43981.05859,"Abundance..130C":51416.92439,"Abundance..131":47696.8994,"Ions.Score":74,"Identity.Strict":27,"Identity.Relaxed":20,"Expectation.Value":2.55522e-07,"Percolator.q.Value":0,"Percolator.PEP":3.489e-07}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (I2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].yDLLcLEGLVr.[G]","Modifications":"N-Term(TMT6plex); C5(Carbamidomethyl); R11(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q9NSD9","Master.Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","Protein.Accessions":"Q9NSD9","Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":795.44366,"MH...Da.":1589.88005,"Theo..MH...Da.":1589.8798,"DeltaM..ppm.":0.16,"Deltam.z..Da.":0.00013,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":29.22286,"Average.Reporter.S.N":11.9,"Ion.Inject.Time..ms.":7.771,"RT..min.":186.3324,"First.Scan":101324,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_01.raw","File.ID":"F7","Abundance..126":27272.50466,"Abundance..127N":25942.49761,"Abundance..127C":28444.59134,"Abundance..128N":19074.39368,"Abundance..128C":25442.82695,"Abundance..129N":26168.37297,"Abundance..129C":27078.92774,"Abundance..130N":28359.27584,"Abundance..130C":25249.82404,"Abundance..131":21833.66224,"Ions.Score":29,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.01191,"Percolator.q.Value":0,"Percolator.PEP":0.0001}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (I2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].iMNVIGEPIDEr.[G]","Modifications":"N-Term(TMT6plex); R12(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":0.9259,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":812.94427,"MH...Da.":1624.88127,"Theo..MH...Da.":1624.88053,"DeltaM..ppm.":0.46,"Deltam.z..Da.":0.00037,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":19.96109,"Average.Reporter.S.N":8.2,"Ion.Inject.Time..ms.":1.917,"RT..min.":154.097,"First.Scan":82297,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_01.raw","File.ID":"F7","Abundance..126":96323.83875,"Abundance..127N":62872.98795,"Abundance..127C":85156.51638,"Abundance..128N":104105.57265,"Abundance..128C":78120.72477,"Abundance..129N":69929.45041,"Abundance..129C":87776.95438,"Abundance..130N":117451.49085,"Abundance..130C":93022.26766,"Abundance..131":51303.93128,"Ions.Score":81,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":5.64275e-08,"Percolator.q.Value":0,"Percolator.PEP":5.894e-06}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (H2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[R].vIISAPSADAPmFVMGVNHEk.[Y]","Modifications":"N-Term(TMT6plex); M12(Oxidation); K21(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.3077,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":899.15436,"MH...Da.":2695.44852,"Theo..MH...Da.":2695.4443,"DeltaM..ppm.":1.57,"Deltam.z..Da.":0.00141,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":20.66338,"Average.Reporter.S.N":20.9,"Ion.Inject.Time..ms.":6.483,"RT..min.":154.9915,"First.Scan":77646,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_02.raw","File.ID":"F8","Abundance..126":104860.91911,"Abundance..127N":88270.08969,"Abundance..127C":94179.96584,"Abundance..128N":83610.98827,"Abundance..128C":108911.40048,"Abundance..129N":93736.20285,"Abundance..129C":98460.94302,"Abundance..130N":88368.48451,"Abundance..130C":112679.75217,"Abundance..131":98318.35846,"Ions.Score":65,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":2.75523e-06,"Percolator.q.Value":0,"Percolator.PEP":7.653e-08}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (H2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].vIISAPSADAPMFVMGVNHEk.[Y]","Modifications":"N-Term(TMT6plex); K21(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":0,"Charge":4,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":670.61908,"MH...Da.":2679.45449,"Theo..MH...Da.":2679.44938,"DeltaM..ppm.":1.9,"Deltam.z..Da.":0.00128,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":10.95414,"Average.Reporter.S.N":72.6,"Ion.Inject.Time..ms.":0.547,"RT..min.":165.2002,"First.Scan":83532,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_02.raw","File.ID":"F8","Abundance..126":2958518.8889,"Abundance..127N":2873886.9438,"Abundance..127C":3021979.12253,"Abundance..128N":3271293.35282,"Abundance..128C":3485123.74741,"Abundance..129N":3012039.01066,"Abundance..129C":3749412.40418,"Abundance..130N":2945069.09519,"Abundance..130C":3414207.96949,"Abundance..131":3494166.0457,"Ions.Score":38,"Identity.Strict":30,"Identity.Relaxed":23,"Expectation.Value":0.00172,"Percolator.q.Value":0,"Percolator.PEP":2.149e-06}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (H2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].wGDAGAEYVVESTGVFTTmEk.[A]","Modifications":"N-Term(TMT6plex); M19(Oxidation); K21(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":920.4704,"MH...Da.":2759.39664,"Theo..MH...Da.":2759.37297,"DeltaM..ppm.":8.58,"Deltam.z..Da.":0.00789,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":0,"Average.Reporter.S.N":9.1,"Ion.Inject.Time..ms.":43.943,"RT..min.":189.7359,"First.Scan":97203,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_02.raw","File.ID":"F8","Abundance..126":2804.8633,"Abundance..127N":3194.74002,"Abundance..127C":3008.15074,"Abundance..128N":2039.72893,"Abundance..128C":2886.88538,"Abundance..129N":3391.41032,"Abundance..129C":3484.95136,"Abundance..130N":2473.57711,"Abundance..130C":2657.25384,"Abundance..131":4509.90864,"Ions.Score":46,"Identity.Strict":26,"Identity.Relaxed":19,"Expectation.Value":0.00012,"Percolator.q.Value":0,"Percolator.PEP":0.00007}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (H2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[R].vPTANVSVVDLTcrLEk.[P]","Modifications":"N-Term(TMT6plex); C13(Carbamidomethyl); R14(Label:13C(6)15N(4)); K17(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":1,"Charge":3,"DeltaScore":0.875,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":793.12744,"MH...Da.":2377.36777,"Theo..MH...Da.":2377.36441,"DeltaM..ppm.":1.42,"Deltam.z..Da.":0.00112,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":20.71376,"Average.Reporter.S.N":21.6,"Ion.Inject.Time..ms.":13.398,"RT..min.":154.6646,"First.Scan":77462,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_02.raw","File.ID":"F8","Abundance..126":37598.65856,"Abundance..127N":31936.23657,"Abundance..127C":44513.50676,"Abundance..128N":41963.00051,"Abundance..128C":36379.33102,"Abundance..129N":29995.60837,"Abundance..129C":32234.23743,"Abundance..130N":39800.535,"Abundance..130C":40125.413,"Abundance..131":32698.65075,"Ions.Score":32,"Identity.Strict":30,"Identity.Relaxed":23,"Expectation.Value":0.00733,"Percolator.q.Value":0,"Percolator.PEP":6.862e-06}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (H2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].iDVPANrYDLLcLEGLVr.[G]","Modifications":"N-Term(TMT6plex); R7(Label:13C(6)15N(4)); C12(Carbamidomethyl); R18(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q9NSD9","Master.Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","Protein.Accessions":"Q9NSD9","Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","X..Missed.Cleavages":1,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":789.10693,"MH...Da.":2365.30625,"Theo..MH...Da.":2365.30141,"DeltaM..ppm.":2.05,"Deltam.z..Da.":0.00161,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":10.83016,"Average.Reporter.S.N":12.5,"Ion.Inject.Time..ms.":4.356,"RT..min.":177.9092,"First.Scan":90724,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_02.raw","File.ID":"F8","Abundance..126":26435.27534,"Abundance..127N":23487.07621,"Abundance..127C":33437.81528,"Abundance..128N":29782.5751,"Abundance..128C":20211.36048,"Abundance..129N":19230.91605,"Abundance..129C":24270.62036,"Abundance..130N":26566.36751,"Abundance..130C":33640.53901,"Abundance..131":27679.88258,"Ions.Score":28,"Identity.Strict":30,"Identity.Relaxed":23,"Expectation.Value":0.01699,"Percolator.q.Value":0,"Percolator.PEP":0.00009}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (H2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].iISNAScTTNcLAPLAk.[V]","Modifications":"N-Term(TMT6plex); C7(Carbamidomethyl); C11(Carbamidomethyl); K17(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.8293,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":767.42639,"MH...Da.":2300.26462,"Theo..MH...Da.":2300.25979,"DeltaM..ppm.":2.1,"Deltam.z..Da.":0.00161,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":5.14124,"Average.Reporter.S.N":122.5,"Ion.Inject.Time..ms.":6.917,"RT..min.":149.3121,"First.Scan":74405,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_02.raw","File.ID":"F8","Abundance..126":939244.23547,"Abundance..127N":786169.81604,"Abundance..127C":969610.53862,"Abundance..128N":931752.33947,"Abundance..128C":887999.39993,"Abundance..129N":784485.9711,"Abundance..129C":971640.92784,"Abundance..130N":889012.56421,"Abundance..130C":838263.71628,"Abundance..131":903886.01449,"Ions.Score":41,"Identity.Strict":30,"Identity.Relaxed":23,"Expectation.Value":0.00095,"Percolator.q.Value":0,"Percolator.PEP":9.812e-08}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (H2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].iPSAVGYQPTLATDMGTMQEr.[I]","Modifications":"N-Term(TMT6plex); R21(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":835.75739,"MH...Da.":2505.2576,"Theo..MH...Da.":2505.25543,"DeltaM..ppm.":0.87,"Deltam.z..Da.":0.00072,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":4.86951,"Average.Reporter.S.N":24.7,"Ion.Inject.Time..ms.":2.901,"RT..min.":162.0911,"First.Scan":81759,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_02.raw","File.ID":"F8","Abundance..126":155277.67578,"Abundance..127N":109594.70048,"Abundance..127C":102973.31634,"Abundance..128N":117526.30842,"Abundance..128C":125289.58152,"Abundance..129N":120580.25635,"Abundance..129C":127728.3932,"Abundance..130N":115301.10893,"Abundance..130C":112905.84775,"Abundance..131":93370.27575,"Ions.Score":59,"Identity.Strict":26,"Identity.Relaxed":19,"Expectation.Value":4.85797e-06,"Percolator.q.Value":0,"Percolator.PEP":1.293e-08}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (H2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[K].drLPLAVVGSNTIIEVNGk.[R]","Modifications":"N-Term(TMT6plex); R2(Label:13C(6)15N(4)); K19(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q16181","Master.Protein.Descriptions":"Septin-7 OS=Homo sapiens GN=SEPT7 PE=1 SV=2","Protein.Accessions":"Q16181","Protein.Descriptions":"Septin-7 OS=Homo sapiens GN=SEPT7 PE=1 SV=2","X..Missed.Cleavages":1,"Charge":3,"DeltaScore":0.9875,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":824.49573,"MH...Da.":2471.47263,"Theo..MH...Da.":2471.47165,"DeltaM..ppm.":0.4,"Deltam.z..Da.":0.00033,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":0,"Average.Reporter.S.N":33.6,"Ion.Inject.Time..ms.":8.202,"RT..min.":157.3759,"First.Scan":79030,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_02.raw","File.ID":"F8","Abundance..126":21333.05681,"Abundance..127N":28793.12285,"Abundance..127C":23156.7694,"Abundance..128N":24216.2856,"Abundance..128C":27550.3109,"Abundance..129N":26950.96544,"Abundance..129C":28380.98401,"Abundance..130N":33772.87265,"Abundance..130C":29492.27868,"Abundance..131":25944.96926,"Ions.Score":80,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":8.04124e-08,"Percolator.q.Value":0,"Percolator.PEP":1.564e-08}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (H2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].fLSQPFQVAEVFTGHMGk.[L]","Modifications":"N-Term(TMT6plex); K18(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":830.45581,"MH...Da.":2489.35288,"Theo..MH...Da.":2489.35066,"DeltaM..ppm.":0.89,"Deltam.z..Da.":0.00074,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":4.30982,"Average.Reporter.S.N":48.5,"Ion.Inject.Time..ms.":4.405,"RT..min.":186.6852,"First.Scan":95565,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_02.raw","File.ID":"F8","Abundance..126":123050.66984,"Abundance..127N":114421.42369,"Abundance..127C":127086.09514,"Abundance..128N":125194.22874,"Abundance..128C":131057.08586,"Abundance..129N":110864.88341,"Abundance..129C":136113.3466,"Abundance..130N":142371.94722,"Abundance..130C":133820.93944,"Abundance..131":123592.04581,"Ions.Score":53,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.00004,"Percolator.q.Value":0,"Percolator.PEP":1.572e-10}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (H2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].fGQAATMEGIGAIGGTPPAFNr.[A]","Modifications":"N-Term(TMT6plex); R22(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q15233","Master.Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","Protein.Accessions":"Q15233","Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":801.41815,"MH...Da.":2402.2399,"Theo..MH...Da.":2402.23635,"DeltaM..ppm.":1.48,"Deltam.z..Da.":0.00118,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":8.17118,"Average.Reporter.S.N":15.5,"Ion.Inject.Time..ms.":4.224,"RT..min.":169.2753,"First.Scan":85859,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_02.raw","File.ID":"F8","Abundance..126":55386.3526,"Abundance..127N":60930.97479,"Abundance..127C":56922.63955,"Abundance..128N":58383.63909,"Abundance..128C":54699.20817,"Abundance..129N":71048.55066,"Abundance..129C":53758.76242,"Abundance..130N":56485.28033,"Abundance..130C":74250.43651,"Abundance..131":71677.64931,"Ions.Score":54,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":0.00002,"Percolator.q.Value":0,"Percolator.PEP":6.739e-07}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (H2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].lFVGNLPPDITEEEMrk.[L]","Modifications":"N-Term(TMT6plex); R16(Label:13C(6)15N(4)); K17(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q15233","Master.Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","Protein.Accessions":"Q15233","Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","X..Missed.Cleavages":1,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":822.1272,"MH...Da.":2464.36704,"Theo..MH...Da.":2464.36407,"DeltaM..ppm.":1.2,"Deltam.z..Da.":0.00099,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":34.52848,"Average.Reporter.S.N":17.1,"Ion.Inject.Time..ms.":10.701,"RT..min.":162.5212,"First.Scan":82009,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_02.raw","File.ID":"F8","Abundance..126":16405.02885,"Abundance..127N":10682.36144,"Abundance..127C":11409.97304,"Abundance..128N":14064.42689,"Abundance..128C":10759.0243,"Abundance..129N":12944.10392,"Abundance..129C":9626.84079,"Abundance..130N":15212.34455,"Abundance..130C":15097.78776,"Abundance..131":11771.85076,"Ions.Score":42,"Identity.Strict":30,"Identity.Relaxed":23,"Expectation.Value":0.0007,"Percolator.q.Value":0,"Percolator.PEP":3.092e-06}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (H2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].lFVGNLPPDITEEEMr.[K]","Modifications":"N-Term(TMT6plex); R16(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q15233","Master.Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","Protein.Accessions":"Q15233","Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":1050.05188,"MH...Da.":2099.09648,"Theo..MH...Da.":2099.09198,"DeltaM..ppm.":2.15,"Deltam.z..Da.":0.00225,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":0,"Average.Reporter.S.N":10.9,"Ion.Inject.Time..ms.":49.497,"RT..min.":176.9284,"First.Scan":90203,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_02.raw","File.ID":"F8","Abundance..126":3031.05171,"Abundance..127N":3808.62202,"Abundance..127C":4190.35409,"Abundance..128N":3350.90403,"Abundance..128C":3252.31851,"Abundance..129N":3548.92946,"Abundance..129C":3901.14214,"Abundance..130N":3967.866,"Abundance..130C":3364.00075,"Abundance..131":3445.55466,"Ions.Score":72,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":3.95161e-07,"Percolator.q.Value":0,"Percolator.PEP":4.526e-07}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (H2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].lFVGNLPPDITEEEMr.[K]","Modifications":"N-Term(TMT6plex); R16(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q15233","Master.Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","Protein.Accessions":"Q15233","Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":1050.05371,"MH...Da.":2099.10015,"Theo..MH...Da.":2099.09198,"DeltaM..ppm.":3.89,"Deltam.z..Da.":0.00408,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":14.06506,"Average.Reporter.S.N":9.4,"Ion.Inject.Time..ms.":34.459,"RT..min.":178.6686,"First.Scan":91167,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_02.raw","File.ID":"F8","Abundance..126":2288.46696,"Abundance..127N":4516.96796,"Abundance..127C":2337.45705,"Abundance..128N":3524.39642,"Abundance..128C":2911.02751,"Abundance..129N":4481.9733,"Abundance..129C":2606.80697,"Abundance..130N":3315.15549,"Abundance..130C":2729.08212,"Abundance..131":3096.21605,"Ions.Score":44,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":0.00031,"Percolator.q.Value":0,"Percolator.PEP":0.0001}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (H2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[R].iMDPNIVGSEHYDVAr.[G]","Modifications":"N-Term(TMT6plex); R16(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":685.6853,"MH...Da.":2055.04135,"Theo..MH...Da.":2055.04061,"DeltaM..ppm.":0.36,"Deltam.z..Da.":0.00025,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":8.54432,"Average.Reporter.S.N":15.5,"Ion.Inject.Time..ms.":10.389,"RT..min.":130.2395,"First.Scan":63634,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_02.raw","File.ID":"F8","Abundance..126":34708.26146,"Abundance..127N":31829.12959,"Abundance..127C":33015.92152,"Abundance..128N":38517.46368,"Abundance..128C":35315.12732,"Abundance..129N":39128.71418,"Abundance..129C":42152.07859,"Abundance..130N":33995.43837,"Abundance..130C":36446.76988,"Abundance..131":31750.74643,"Ions.Score":53,"Identity.Strict":26,"Identity.Relaxed":19,"Expectation.Value":0.00002,"Percolator.q.Value":0,"Percolator.PEP":4.162e-07}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (H2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].gTGGVDTAAVGGVFDVSNADr.[L]","Modifications":"N-Term(TMT6plex); R21(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P12277","Master.Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","Protein.Accessions":"P12277","Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":735.37292,"MH...Da.":2204.10422,"Theo..MH...Da.":2204.10202,"DeltaM..ppm.":1,"Deltam.z..Da.":0.00073,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":22.36478,"Average.Reporter.S.N":14,"Ion.Inject.Time..ms.":6.305,"RT..min.":149.2235,"First.Scan":74353,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_02.raw","File.ID":"F8","Abundance..126":25862.50387,"Abundance..127N":26671.13455,"Abundance..127C":35579.46152,"Abundance..128N":33581.5632,"Abundance..128C":27689.11246,"Abundance..129N":27416.81229,"Abundance..129C":28982.54483,"Abundance..130N":32349.83901,"Abundance..130C":26836.02962,"Abundance..131":25561.81305,"Ions.Score":35,"Identity.Strict":26,"Identity.Relaxed":19,"Expectation.Value":0.00132,"Percolator.q.Value":0,"Percolator.PEP":5.21e-07}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (I2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].vPTANVSVVDLTcr.[L]","Modifications":"N-Term(TMT6plex); C13(Carbamidomethyl); R14(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":0.9846,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":885.48767,"MH...Da.":1769.96807,"Theo..MH...Da.":1769.96565,"DeltaM..ppm.":1.36,"Deltam.z..Da.":0.00121,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":18.04383,"Average.Reporter.S.N":3.6,"Ion.Inject.Time..ms.":8.411,"RT..min.":145.7987,"First.Scan":77344,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_01.raw","File.ID":"F7","Abundance..126":27030.54814,"Abundance..127N":17165.85223,"Abundance..127C":20193.0784,"Abundance..128N":17767.8322,"Abundance..128C":21435.70703,"Abundance..129C":20305.21994,"Abundance..130N":17747.59732,"Abundance..130C":19978.08928,"Abundance..131":21844.15001,"Ions.Score":65,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":2.63936e-06,"Percolator.q.Value":0,"Percolator.PEP":1.091e-06}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (G2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].tIAMDGTEGLVr.[G]","Modifications":"N-Term(TMT6plex); R12(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":0.9467,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":751.41034,"MH...Da.":1501.8134,"Theo..MH...Da.":1501.81211,"DeltaM..ppm.":0.86,"Deltam.z..Da.":0.00064,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":26.00055,"Average.Reporter.S.N":41.3,"Ion.Inject.Time..ms.":5.847,"RT..min.":125.8898,"First.Scan":60780,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_02.raw","File.ID":"F9","Abundance..126":128450.11971,"Abundance..127N":93487.6709,"Abundance..127C":117081.8032,"Abundance..128N":108142.78572,"Abundance..128C":90723.67943,"Abundance..129N":116029.73204,"Abundance..129C":125112.14453,"Abundance..130N":123669.82556,"Abundance..130C":126362.68272,"Abundance..131":100998.55945,"Ions.Score":75,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":2.30758e-07,"Percolator.q.Value":0,"Percolator.PEP":1.241e-06}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (J2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].iISNAScTTNcLAPLAk.[V]","Modifications":"N-Term(TMT6plex); C7(Carbamidomethyl); C11(Carbamidomethyl); K17(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.75,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":767.42572,"MH...Da.":2300.26261,"Theo..MH...Da.":2300.25979,"DeltaM..ppm.":1.22,"Deltam.z..Da.":0.00094,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":13.83713,"Average.Reporter.S.N":140.2,"Ion.Inject.Time..ms.":3.956,"RT..min.":149.326,"First.Scan":77518,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture4_03.raw","File.ID":"F6","Abundance..126":1683876.57674,"Abundance..127N":1633543.33366,"Abundance..127C":1553176.97328,"Abundance..128N":1635168.98373,"Abundance..128C":1591971.57004,"Abundance..129N":1354964.13119,"Abundance..129C":1736790.27829,"Abundance..130N":1606130.44774,"Abundance..130C":1481778.98498,"Abundance..131":1351478.64714,"Ions.Score":32,"Identity.Strict":30,"Identity.Relaxed":23,"Expectation.Value":0.00816,"Percolator.q.Value":0,"Percolator.PEP":2.16e-07}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (J2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[K].nLEGYVGFANLPNQVYr.[K]","Modifications":"N-Term(TMT6plex); R17(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q16181","Master.Protein.Descriptions":"Septin-7 OS=Homo sapiens GN=SEPT7 PE=1 SV=2","Protein.Accessions":"Q16181","Protein.Descriptions":"Septin-7 OS=Homo sapiens GN=SEPT7 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.9737,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":731.72278,"MH...Da.":2193.15378,"Theo..MH...Da.":2193.15294,"DeltaM..ppm.":0.39,"Deltam.z..Da.":0.00028,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":4.28659,"Average.Reporter.S.N":17.5,"Ion.Inject.Time..ms.":3.409,"RT..min.":169.5479,"First.Scan":89508,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture4_03.raw","File.ID":"F6","Abundance..126":90160.21347,"Abundance..127N":112158.53719,"Abundance..127C":101104.51275,"Abundance..128N":86967.49471,"Abundance..128C":83700.25586,"Abundance..129N":92063.19002,"Abundance..129C":92241.0664,"Abundance..130N":99316.06332,"Abundance..130C":83574.66958,"Abundance..131":96397.82942,"Ions.Score":38,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":0.00103,"Percolator.q.Value":0,"Percolator.PEP":3.398e-06}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (J2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].iYEFPETDDEEENk.[L]","Modifications":"N-Term(TMT6plex); K14(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q16181","Master.Protein.Descriptions":"Septin-7 OS=Homo sapiens GN=SEPT7 PE=1 SV=2","Protein.Accessions":"Q16181","Protein.Descriptions":"Septin-7 OS=Homo sapiens GN=SEPT7 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":1112.54712,"MH...Da.":2224.08696,"Theo..MH...Da.":2224.07889,"DeltaM..ppm.":3.63,"Deltam.z..Da.":0.00403,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":0,"Average.Reporter.S.N":26.3,"Ion.Inject.Time..ms.":50,"RT..min.":147.0875,"First.Scan":76201,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture4_03.raw","File.ID":"F6","Abundance..126":8533.49864,"Abundance..127N":8124.78426,"Abundance..127C":6363.56695,"Abundance..128N":10246.22959,"Abundance..128C":9650.40972,"Abundance..129N":9024.04713,"Abundance..129C":8047.91451,"Abundance..130N":9313.04955,"Abundance..130C":10029.77303,"Abundance..131":8048.40889,"Ions.Score":42,"Identity.Strict":25,"Identity.Relaxed":18,"Expectation.Value":0.00021,"Percolator.q.Value":0,"Percolator.PEP":0.00002}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (J2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].aIAELGIYPAVDPLDSTSr.[I]","Modifications":"N-Term(TMT6plex); R19(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":1114.10803,"MH...Da.":2227.20879,"Theo..MH...Da.":2227.2047,"DeltaM..ppm.":1.84,"Deltam.z..Da.":0.00204,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":10.90336,"Average.Reporter.S.N":33.1,"Ion.Inject.Time..ms.":10.871,"RT..min.":184.8169,"First.Scan":98440,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture4_03.raw","File.ID":"F6","Abundance..126":91792.56693,"Abundance..127N":79023.84403,"Abundance..127C":65209.69076,"Abundance..128N":94367.27497,"Abundance..128C":86991.02881,"Abundance..129N":73240.7595,"Abundance..129C":94504.82547,"Abundance..130N":82695.45707,"Abundance..130C":73568.09538,"Abundance..131":71040.42974,"Ions.Score":55,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.00003,"Percolator.q.Value":0,"Percolator.PEP":1.808e-06}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (J2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[K].lVINGNPITIFQErDPSk.[I]","Modifications":"N-Term(TMT6plex); R14(Label:13C(6)15N(4)); K18(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":1,"Charge":3,"DeltaScore":0.9016,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":839.82483,"MH...Da.":2517.45993,"Theo..MH...Da.":2517.456,"DeltaM..ppm.":1.56,"Deltam.z..Da.":0.00131,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":12.78139,"Average.Reporter.S.N":44.6,"Ion.Inject.Time..ms.":3.989,"RT..min.":165.8389,"First.Scan":87319,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture4_03.raw","File.ID":"F6","Abundance..126":585471.00445,"Abundance..127N":527590.04302,"Abundance..127C":560246.78945,"Abundance..128N":548171.27541,"Abundance..128C":388275.15154,"Abundance..129N":566187.75868,"Abundance..129C":483575.43218,"Abundance..130N":575523.92113,"Abundance..130C":529196.09887,"Abundance..131":554440.31873,"Ions.Score":61,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":6.94934e-06,"Percolator.q.Value":0,"Percolator.PEP":2.23e-09}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (J2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].iPSAVGYQPTLATDMGTMQEr.[I]","Modifications":"N-Term(TMT6plex); R21(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":835.75873,"MH...Da.":2505.26163,"Theo..MH...Da.":2505.25543,"DeltaM..ppm.":2.48,"Deltam.z..Da.":0.00207,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":14.22178,"Average.Reporter.S.N":24.3,"Ion.Inject.Time..ms.":2.364,"RT..min.":162.0632,"First.Scan":85085,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture4_03.raw","File.ID":"F6","Abundance..126":180492.76415,"Abundance..127N":200530.39958,"Abundance..127C":175857.9846,"Abundance..128N":195091.98476,"Abundance..128C":213941.15152,"Abundance..129N":183917.96544,"Abundance..129C":169949.40901,"Abundance..130N":178042.30754,"Abundance..130C":180162.30029,"Abundance..131":219786.25611,"Ions.Score":70,"Identity.Strict":26,"Identity.Relaxed":19,"Expectation.Value":4.30912e-07,"Percolator.q.Value":0,"Percolator.PEP":1.042e-09}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (J2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].eGNDLYHEMIESGVINLk.[D]","Modifications":"N-Term(TMT6plex); K18(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.9231,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":843.11737,"MH...Da.":2527.33756,"Theo..MH...Da.":2527.33579,"DeltaM..ppm.":0.7,"Deltam.z..Da.":0.00059,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":43.3462,"Average.Reporter.S.N":27.4,"Ion.Inject.Time..ms.":4.526,"RT..min.":173.1874,"First.Scan":91654,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture4_03.raw","File.ID":"F6","Abundance..126":63951.71788,"Abundance..127N":67072.58955,"Abundance..127C":63018.94304,"Abundance..128N":69111.76874,"Abundance..128C":68469.21935,"Abundance..129N":55455.83776,"Abundance..129C":75986.21194,"Abundance..130N":74228.48694,"Abundance..130C":71213.64743,"Abundance..131":65468.75669,"Ions.Score":39,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.00099,"Percolator.q.Value":0,"Percolator.PEP":1.249e-07}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (G2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].rDLLFQALGr.[T]","Modifications":"N-Term(TMT6plex); R1(Label:13C(6)15N(4)); R10(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q9NSD9","Master.Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","Protein.Accessions":"Q9NSD9","Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","X..Missed.Cleavages":1,"Charge":3,"DeltaScore":0.9388,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":479.95975,"MH...Da.":1437.86469,"Theo..MH...Da.":1437.86424,"DeltaM..ppm.":0.31,"Deltam.z..Da.":0.00015,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":44.95973,"Average.Reporter.S.N":24.1,"Ion.Inject.Time..ms.":15.564,"RT..min.":136.2664,"First.Scan":66560,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_02.raw","File.ID":"F9","Abundance..126":23608.90099,"Abundance..127N":18455.15719,"Abundance..127C":21440.78092,"Abundance..128N":17222.90211,"Abundance..128C":19987.91007,"Abundance..129N":25806.63148,"Abundance..129C":26517.41478,"Abundance..130N":20884.38362,"Abundance..130C":20702.27759,"Abundance..131":17238.53629,"Ions.Score":49,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.00009,"Percolator.q.Value":0,"Percolator.PEP":0.00001}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (J2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].lFVGNLPPDITEEEMrk.[L]","Modifications":"N-Term(TMT6plex); R16(Label:13C(6)15N(4)); K17(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q15233","Master.Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","Protein.Accessions":"Q15233","Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","X..Missed.Cleavages":1,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":822.12585,"MH...Da.":2464.36301,"Theo..MH...Da.":2464.36407,"DeltaM..ppm.":-0.43,"Deltam.z..Da.":-0.00035,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":11.22622,"Average.Reporter.S.N":14.4,"Ion.Inject.Time..ms.":5.487,"RT..min.":162.72,"First.Scan":85476,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture4_03.raw","File.ID":"F6","Abundance..126":38332.77563,"Abundance..127N":39826.7458,"Abundance..127C":33230.56028,"Abundance..128N":36851.16949,"Abundance..128C":32487.12637,"Abundance..129N":40174.57869,"Abundance..129C":29681.31551,"Abundance..130N":35305.5906,"Abundance..130C":49458.42851,"Abundance..131":45829.50849,"Ions.Score":31,"Identity.Strict":30,"Identity.Relaxed":23,"Expectation.Value":0.00881,"Percolator.q.Value":0,"Percolator.PEP":0.00002}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (G2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[K].lVEALcAAGHr.[A]","Modifications":"N-Term(TMT6plex); C6(Carbamidomethyl); R11(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P23919","Master.Protein.Descriptions":"Thymidylate kinase OS=Homo sapiens GN=DTYMK PE=1 SV=4","Protein.Accessions":"P23919","Protein.Descriptions":"Thymidylate kinase OS=Homo sapiens GN=DTYMK PE=1 SV=4","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":479.26825,"MH...Da.":1435.7902,"Theo..MH...Da.":1435.79165,"DeltaM..ppm.":-1.02,"Deltam.z..Da.":-0.00049,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":40.38025,"Average.Reporter.S.N":46.8,"Ion.Inject.Time..ms.":7.299,"RT..min.":103.7054,"First.Scan":48478,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_02.raw","File.ID":"F9","Abundance..126":83288.41548,"Abundance..127N":84803.94283,"Abundance..127C":85333.88665,"Abundance..128N":81672.14758,"Abundance..128C":81997.61302,"Abundance..129N":77433.69666,"Abundance..129C":76475.73801,"Abundance..130N":66457.57224,"Abundance..130C":76932.14148,"Abundance..131":83588.58701,"Ions.Score":39,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":0.00104,"Percolator.q.Value":0,"Percolator.PEP":2.527e-06}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (J2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].fLSQPFQVAEVFTGHMGk.[L]","Modifications":"N-Term(TMT6plex); K18(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":830.45648,"MH...Da.":2489.35489,"Theo..MH...Da.":2489.35066,"DeltaM..ppm.":1.7,"Deltam.z..Da.":0.00141,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":2.17244,"Average.Reporter.S.N":50.3,"Ion.Inject.Time..ms.":0.866,"RT..min.":186.6045,"First.Scan":99470,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture4_03.raw","File.ID":"F6","Abundance..126":737437.34286,"Abundance..127N":912769.72211,"Abundance..127C":929168.00836,"Abundance..128N":970007.54698,"Abundance..128C":972117.38013,"Abundance..129N":993171.82548,"Abundance..129C":1042135.25191,"Abundance..130N":1015410.09266,"Abundance..130C":1032734.04924,"Abundance..131":926771.61385,"Ions.Score":70,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":9.0529e-07,"Percolator.q.Value":0,"Percolator.PEP":7.1e-11}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (G2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].sIEAVHEDIr.[V]","Modifications":"N-Term(TMT6plex); R10(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P23919","Master.Protein.Descriptions":"Thymidylate kinase OS=Homo sapiens GN=DTYMK PE=1 SV=4","Protein.Accessions":"P23919","Protein.Descriptions":"Thymidylate kinase OS=Homo sapiens GN=DTYMK PE=1 SV=4","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.8182,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":469.92697,"MH...Da.":1407.76636,"Theo..MH...Da.":1407.76688,"DeltaM..ppm.":-0.37,"Deltam.z..Da.":-0.00017,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":26.12567,"Average.Reporter.S.N":71.3,"Ion.Inject.Time..ms.":14.074,"RT..min.":70.7887,"First.Scan":30554,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_02.raw","File.ID":"F9","Abundance..126":92158.33239,"Abundance..127N":94635.37064,"Abundance..127C":94018.28879,"Abundance..128N":108487.08673,"Abundance..128C":100734.52744,"Abundance..129N":110846.22278,"Abundance..129C":114137.79222,"Abundance..130N":106990.62847,"Abundance..130C":114373.09433,"Abundance..131":99070.91892,"Ions.Score":22,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":0.04045,"Percolator.q.Value":0,"Percolator.PEP":0.00029}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (J2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[R].vPTANVSVVDLTcrLEk.[P]","Modifications":"N-Term(TMT6plex); C13(Carbamidomethyl); R14(Label:13C(6)15N(4)); K17(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":1,"Charge":3,"DeltaScore":0.9211,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":793.12653,"MH...Da.":2377.36502,"Theo..MH...Da.":2377.36441,"DeltaM..ppm.":0.26,"Deltam.z..Da.":0.00021,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":17.46093,"Average.Reporter.S.N":24.7,"Ion.Inject.Time..ms.":5.735,"RT..min.":154.2781,"First.Scan":80465,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture4_03.raw","File.ID":"F6","Abundance..126":51644.07284,"Abundance..127N":65678.62649,"Abundance..127C":57662.4491,"Abundance..128N":61509.47752,"Abundance..128C":54832.72732,"Abundance..129N":54285.23877,"Abundance..129C":61845.20425,"Abundance..130N":50747.80908,"Abundance..130C":47094.20697,"Abundance..131":55563.5559,"Ions.Score":38,"Identity.Strict":30,"Identity.Relaxed":23,"Expectation.Value":0.00157,"Percolator.q.Value":0,"Percolator.PEP":0.00002}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (J2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].rGTGGVDTAAVGGVFDVSNADr.[L]","Modifications":"N-Term(TMT6plex); R1(Label:13C(6)15N(4)); R22(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P12277","Master.Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","Protein.Accessions":"P12277","Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":790.74213,"MH...Da.":2370.21183,"Theo..MH...Da.":2370.2114,"DeltaM..ppm.":0.18,"Deltam.z..Da.":0.00014,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":0,"Average.Reporter.S.N":19.7,"Ion.Inject.Time..ms.":26.796,"RT..min.":119.9263,"First.Scan":60265,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture4_03.raw","File.ID":"F6","Abundance..126":8014.23387,"Abundance..127N":10358.50875,"Abundance..127C":9038.87608,"Abundance..128N":11887.16834,"Abundance..128C":11239.88224,"Abundance..129N":12412.17023,"Abundance..129C":9903.19584,"Abundance..130N":11595.21345,"Abundance..130C":9782.97569,"Abundance..131":10749.45469,"Ions.Score":64,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":2.57084e-06,"Percolator.q.Value":0,"Percolator.PEP":8.344e-09}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (J2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].iDVPANrYDLLcLEGLVr.[G]","Modifications":"N-Term(TMT6plex); R7(Label:13C(6)15N(4)); C12(Carbamidomethyl); R18(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q9NSD9","Master.Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","Protein.Accessions":"Q9NSD9","Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","X..Missed.Cleavages":1,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":789.10547,"MH...Da.":2365.30185,"Theo..MH...Da.":2365.30141,"DeltaM..ppm.":0.19,"Deltam.z..Da.":0.00015,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":17.45817,"Average.Reporter.S.N":9.3,"Ion.Inject.Time..ms.":4.028,"RT..min.":177.7892,"First.Scan":94342,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture4_03.raw","File.ID":"F6","Abundance..126":34474.90275,"Abundance..127N":36511.79186,"Abundance..127C":25622.28594,"Abundance..128N":47557.16897,"Abundance..128C":39356.49585,"Abundance..129N":31066.15791,"Abundance..129C":38826.07646,"Abundance..130N":34472.12104,"Abundance..130C":36071.3981,"Abundance..131":28999.04311,"Ions.Score":30,"Identity.Strict":30,"Identity.Relaxed":23,"Expectation.Value":0.01125,"Percolator.q.Value":0,"Percolator.PEP":0.00015}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (G2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[K].iGLFGGAGVGk.[T]","Modifications":"N-Term(TMT6plex); K11(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":0.8814,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":721.45557,"MH...Da.":1441.90386,"Theo..MH...Da.":1441.90226,"DeltaM..ppm.":1.11,"Deltam.z..Da.":0.0008,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":31.45217,"Average.Reporter.S.N":69,"Ion.Inject.Time..ms.":2.927,"RT..min.":152.4912,"First.Scan":75761,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_02.raw","File.ID":"F9","Abundance..126":539962.74314,"Abundance..127N":546061.73197,"Abundance..127C":594490.76746,"Abundance..128N":575314.49982,"Abundance..128C":581524.07539,"Abundance..129N":601007.13288,"Abundance..129C":544151.29694,"Abundance..130N":534987.92024,"Abundance..130C":492645.99457,"Abundance..131":582833.65853,"Ions.Score":59,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":8.67478e-06,"Percolator.q.Value":0,"Percolator.PEP":0.00021}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (J2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].eYTAcELMNIYk.[T]","Modifications":"N-Term(TMT6plex); C5(Carbamidomethyl); K12(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q9NSD9","Master.Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","Protein.Accessions":"Q9NSD9","Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":0.913,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":1001.0202,"MH...Da.":2001.03313,"Theo..MH...Da.":2001.03169,"DeltaM..ppm.":0.72,"Deltam.z..Da.":0.00072,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":54.76504,"Average.Reporter.S.N":4.9,"Ion.Inject.Time..ms.":12.164,"RT..min.":162.923,"First.Scan":85602,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture4_03.raw","File.ID":"F6","Abundance..126":3185.1197,"Abundance..127N":6786.53601,"Abundance..127C":8239.2258,"Abundance..128N":6357.09584,"Abundance..128C":7572.23166,"Abundance..129N":3426.13164,"Abundance..129C":4459.40552,"Abundance..130N":3959.433,"Abundance..130C":6601.37219,"Abundance..131":4906.33759,"Ions.Score":46,"Identity.Strict":27,"Identity.Relaxed":20,"Expectation.Value":0.00013,"Percolator.q.Value":0,"Percolator.PEP":2.22e-07}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (J2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].lISWYDNEFGYSNr.[V]","Modifications":"N-Term(TMT6plex); R14(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":668.33032,"MH...Da.":2002.97641,"Theo..MH...Da.":2002.97358,"DeltaM..ppm.":1.42,"Deltam.z..Da.":0.00095,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":13.01973,"Average.Reporter.S.N":50.4,"Ion.Inject.Time..ms.":4.256,"RT..min.":177.166,"First.Scan":93973,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture4_03.raw","File.ID":"F6","Abundance..126":409819.56023,"Abundance..127N":378022.40422,"Abundance..127C":348600.4319,"Abundance..128N":392500.29454,"Abundance..128C":344425.868,"Abundance..129N":328923.09981,"Abundance..129C":439456.33047,"Abundance..130N":403852.27334,"Abundance..130C":319571.28976,"Abundance..131":370015.22417,"Ions.Score":32,"Identity.Strict":24,"Identity.Relaxed":17,"Expectation.Value":0.00199,"Percolator.q.Value":0,"Percolator.PEP":5.279e-06}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (J2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[R].fAQPGSFEYEYAMr.[W]","Modifications":"N-Term(TMT6plex); R14(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q15233","Master.Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","Protein.Accessions":"Q15233","Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":967.96625,"MH...Da.":1934.92522,"Theo..MH...Da.":1934.91837,"DeltaM..ppm.":3.54,"Deltam.z..Da.":0.00342,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":0,"Average.Reporter.S.N":18.5,"Ion.Inject.Time..ms.":34.68,"RT..min.":154.0923,"First.Scan":80356,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture4_03.raw","File.ID":"F6","Abundance..126":6245.7682,"Abundance..127N":9544.22151,"Abundance..127C":8104.99597,"Abundance..128N":7433.68661,"Abundance..128C":9883.86971,"Abundance..129N":9378.37411,"Abundance..129C":8874.3798,"Abundance..130N":6469.44139,"Abundance..130C":6927.47344,"Abundance..131":7039.85958,"Ions.Score":80,"Identity.Strict":21,"Identity.Relaxed":14,"Expectation.Value":1.50083e-08,"Percolator.q.Value":0,"Percolator.PEP":8.629e-06}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (G2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].yAFSGVAFTGAk.[E]","Modifications":"N-Term(TMT6plex); K12(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P23919","Master.Protein.Descriptions":"Thymidylate kinase OS=Homo sapiens GN=DTYMK PE=1 SV=4","Protein.Accessions":"P23919","Protein.Descriptions":"Thymidylate kinase OS=Homo sapiens GN=DTYMK PE=1 SV=4","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":842.98138,"MH...Da.":1684.95549,"Theo..MH...Da.":1684.95541,"DeltaM..ppm.":0.05,"Deltam.z..Da.":0.00004,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":28.88273,"Average.Reporter.S.N":27.3,"Ion.Inject.Time..ms.":12.225,"RT..min.":152.2217,"First.Scan":75603,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_02.raw","File.ID":"F9","Abundance..126":30871.0639,"Abundance..127N":35734.00331,"Abundance..127C":32200.38729,"Abundance..128N":32691.7247,"Abundance..128C":38190.19827,"Abundance..129N":34823.89334,"Abundance..129C":34486.75387,"Abundance..130N":33373.05186,"Abundance..130C":34299.27345,"Abundance..131":26930.59354,"Ions.Score":58,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.00002,"Percolator.q.Value":0,"Percolator.PEP":2.531e-07}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (G2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].vVDLMAHmASk.[E]","Modifications":"N-Term(TMT6plex); M8(Oxidation); K11(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.8889,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":561.98511,"MH...Da.":1683.94077,"Theo..MH...Da.":1683.94175,"DeltaM..ppm.":-0.58,"Deltam.z..Da.":-0.00033,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":27.39447,"Average.Reporter.S.N":29.8,"Ion.Inject.Time..ms.":10.147,"RT..min.":110.0353,"First.Scan":51972,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_02.raw","File.ID":"F9","Abundance..126":73062.19245,"Abundance..127N":71904.50598,"Abundance..127C":71955.2322,"Abundance..128N":76674.98747,"Abundance..128C":75467.40664,"Abundance..129N":61236.77958,"Abundance..129C":73576.39411,"Abundance..130N":86201.44484,"Abundance..130C":78529.13812,"Abundance..131":78815.74762,"Ions.Score":18,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.1297,"Percolator.q.Value":0,"Percolator.PEP":0.00025}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (G2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].vVDLMAHMASk.[E]","Modifications":"N-Term(TMT6plex); K11(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.68,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":556.65381,"MH...Da.":1667.94687,"Theo..MH...Da.":1667.94684,"DeltaM..ppm.":0.02,"Deltam.z..Da.":1e-05,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":3.55294,"Average.Reporter.S.N":129.2,"Ion.Inject.Time..ms.":4.592,"RT..min.":146.7764,"First.Scan":72482,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_02.raw","File.ID":"F9","Abundance..126":980432.04449,"Abundance..127N":1103854.72356,"Abundance..127C":1035580.70953,"Abundance..128N":1026198.97232,"Abundance..128C":1001273.64532,"Abundance..129N":987806.39999,"Abundance..129C":1280031.19287,"Abundance..130N":1111164.96311,"Abundance..130C":1019350.52634,"Abundance..131":1057462.33312,"Ions.Score":25,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.02907,"Percolator.q.Value":0,"Percolator.PEP":0.00019}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (J2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].fAQPGSFEYEYAmr.[W]","Modifications":"N-Term(TMT6plex); M13(Oxidation); R14(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q15233","Master.Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","Protein.Accessions":"Q15233","Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":650.97681,"MH...Da.":1950.91587,"Theo..MH...Da.":1950.91328,"DeltaM..ppm.":1.32,"Deltam.z..Da.":0.00086,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":43.7956,"Average.Reporter.S.N":16.1,"Ion.Inject.Time..ms.":3.698,"RT..min.":142.2061,"First.Scan":73318,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture4_03.raw","File.ID":"F6","Abundance..126":78981.22199,"Abundance..127N":111763.1142,"Abundance..127C":122778.46748,"Abundance..128N":124142.37415,"Abundance..128C":125075.60924,"Abundance..129N":107585.36382,"Abundance..129C":113529.90292,"Abundance..130N":110717.20199,"Abundance..130C":122064.4263,"Abundance..131":108282.74669,"Ions.Score":25,"Identity.Strict":23,"Identity.Relaxed":16,"Expectation.Value":0.0065,"Percolator.q.Value":0,"Percolator.PEP":5.194e-07}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (G2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].vALTGLTVAEYFr.[D]","Modifications":"N-Term(TMT6plex); R13(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":839.98444,"MH...Da.":1678.9616,"Theo..MH...Da.":1678.96049,"DeltaM..ppm.":0.66,"Deltam.z..Da.":0.00055,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":25.92824,"Average.Reporter.S.N":7.7,"Ion.Inject.Time..ms.":23.604,"RT..min.":181.718,"First.Scan":92393,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_02.raw","File.ID":"F9","Abundance..126":14946.31859,"Abundance..127N":11478.74007,"Abundance..127C":15369.59632,"Abundance..128N":15321.88082,"Abundance..128C":15864.70377,"Abundance..129N":19108.22362,"Abundance..129C":19693.23803,"Abundance..130N":12656.61658,"Abundance..130C":9817.04439,"Abundance..131":11737.53647,"Ions.Score":28,"Identity.Strict":30,"Identity.Relaxed":23,"Expectation.Value":0.01696,"Percolator.q.Value":0,"Percolator.PEP":9.392e-06}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (J4)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].dQEGQDVLLFIDNIFR.[F]","Modifications":"N-Term(TMT6plex)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.9355,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":717.71674,"MH...Da.":2151.13565,"Theo..MH...Da.":2151.12835,"DeltaM..ppm.":3.4,"Deltam.z..Da.":0.00243,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":6.9281,"Average.Reporter.S.N":12.5,"Ion.Inject.Time..ms.":48.56,"RT..min.":232.3035,"First.Scan":123115,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture4_03.raw","File.ID":"F6","Abundance..126":4575.3167,"Abundance..127N":3647.87853,"Abundance..127C":4592.03336,"Abundance..128N":4974.90939,"Abundance..128C":4326.93132,"Abundance..129N":4352.40241,"Abundance..129C":4535.96203,"Abundance..130N":3833.24016,"Abundance..130C":4912.97318,"Abundance..131":4289.86179,"Ions.Score":31,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.00636,"Percolator.q.Value":0,"Percolator.PEP":0.02871}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (J2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].hDMAAAGFTEALTFALcSQEDIADk.[L]","Modifications":"N-Term(TMT6plex); C17(Carbamidomethyl); K25(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q9NSD9","Master.Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","Protein.Accessions":"Q9NSD9","Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":1060.19739,"MH...Da.":3178.57761,"Theo..MH...Da.":3178.56806,"DeltaM..ppm.":3.01,"Deltam.z..Da.":0.00318,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":35.96932,"Average.Reporter.S.N":9.6,"Ion.Inject.Time..ms.":44.136,"RT..min.":198.9929,"First.Scan":106491,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture4_03.raw","File.ID":"F6","Abundance..126":3120.89884,"Abundance..127N":2791.26431,"Abundance..127C":2186.64147,"Abundance..128N":2906.16023,"Abundance..128C":3552.53708,"Abundance..129N":3764.54416,"Abundance..129C":3468.52454,"Abundance..130N":3752.66726,"Abundance..130C":3387.23884,"Abundance..131":2959.61828,"Ions.Score":41,"Identity.Strict":26,"Identity.Relaxed":19,"Expectation.Value":0.00037,"Percolator.q.Value":0,"Percolator.PEP":0.00002}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (J2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].rVIISAPSADAPMFVmGVNHEk.[Y]","Modifications":"N-Term(TMT6plex); R1(Label:13C(6)15N(4)); M16(Oxidation); K22(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":1,"Charge":4,"DeltaScore":0.0769,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":716.14569,"MH...Da.":2861.56093,"Theo..MH...Da.":2861.55368,"DeltaM..ppm.":2.54,"Deltam.z..Da.":0.00181,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":20.65285,"Average.Reporter.S.N":26.1,"Ion.Inject.Time..ms.":8.772,"RT..min.":139.233,"First.Scan":71566,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture4_03.raw","File.ID":"F6","Abundance..126":53677.84535,"Abundance..127N":66697.94494,"Abundance..127C":57187.03318,"Abundance..128N":67629.98595,"Abundance..128C":53770.76653,"Abundance..129N":55435.54318,"Abundance..129C":78904.4306,"Abundance..130N":53451.71596,"Abundance..130C":58400.7633,"Abundance..131":58800.56813,"Ions.Score":39,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.00129,"Percolator.q.Value":0,"Percolator.PEP":1.425e-06}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (G2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].trEGNDLYHEMIESGVINLk.[D]","Modifications":"N-Term(TMT6plex); R2(Label:13C(6)15N(4)); K20(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":1,"Charge":4,"DeltaScore":0.9286,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":699.37872,"MH...Da.":2794.49306,"Theo..MH...Da.":2794.49285,"DeltaM..ppm.":0.08,"Deltam.z..Da.":0.00005,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":9.0648,"Average.Reporter.S.N":43.1,"Ion.Inject.Time..ms.":1.41,"RT..min.":156.5885,"First.Scan":78097,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_02.raw","File.ID":"F9","Abundance..126":718139.23686,"Abundance..127N":576622.50256,"Abundance..127C":741079.33856,"Abundance..128N":693643.67628,"Abundance..128C":703882.86052,"Abundance..129N":672537.84247,"Abundance..129C":757744.96937,"Abundance..130N":717431.98748,"Abundance..130C":743021.35177,"Abundance..131":687775.45672,"Ions.Score":14,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.34075,"Percolator.q.Value":0,"Percolator.PEP":0.00005}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (G2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].smTEAEQQQLIDDHFLFDk.[P]","Modifications":"N-Term(TMT6plex); M2(Oxidation); K19(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P12277","Master.Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","Protein.Accessions":"P12277","Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":926.47424,"MH...Da.":2777.40818,"Theo..MH...Da.":2777.39477,"DeltaM..ppm.":4.83,"Deltam.z..Da.":0.00447,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":23.68033,"Average.Reporter.S.N":16.9,"Ion.Inject.Time..ms.":27.732,"RT..min.":166.8865,"First.Scan":84034,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_02.raw","File.ID":"F9","Abundance..126":11449.78642,"Abundance..127N":9647.78821,"Abundance..127C":11002.82408,"Abundance..128N":11628.52821,"Abundance..128C":12986.57935,"Abundance..129N":10111.33572,"Abundance..129C":8973.90586,"Abundance..130N":12452.60732,"Abundance..130C":12662.44274,"Abundance..131":9664.32605,"Ions.Score":33,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":0.00309,"Percolator.q.Value":0,"Percolator.PEP":9.064e-06}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (G2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].rGTGGVDTAAVGGVFDVSNADr.[L]","Modifications":"N-Term(TMT6plex); R1(Label:13C(6)15N(4)); R22(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P12277","Master.Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","Protein.Accessions":"P12277","Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":790.74213,"MH...Da.":2370.21183,"Theo..MH...Da.":2370.2114,"DeltaM..ppm.":0.18,"Deltam.z..Da.":0.00014,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":0,"Average.Reporter.S.N":15.5,"Ion.Inject.Time..ms.":4.031,"RT..min.":120.5788,"First.Scan":57884,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_02.raw","File.ID":"F9","Abundance..126":17524.80688,"Abundance..127N":22959.34017,"Abundance..127C":18492.07787,"Abundance..128N":17776.85059,"Abundance..128C":16484.03484,"Abundance..129N":18277.50308,"Abundance..129C":18779.19368,"Abundance..130N":12585.61946,"Abundance..130C":17712.13128,"Abundance..131":20084.11681,"Ions.Score":42,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":0.00038,"Percolator.q.Value":0,"Percolator.PEP":0.00002}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (G2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[K].iISNAScTTNcLAPLAk.[V]","Modifications":"N-Term(TMT6plex); C7(Carbamidomethyl); C11(Carbamidomethyl); K17(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.7407,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":767.42474,"MH...Da.":2300.25968,"Theo..MH...Da.":2300.25979,"DeltaM..ppm.":-0.05,"Deltam.z..Da.":-0.00004,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":61.64052,"Average.Reporter.S.N":21.8,"Ion.Inject.Time..ms.":14.174,"RT..min.":148.9408,"First.Scan":73730,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_02.raw","File.ID":"F9","Abundance..126":14578.57026,"Abundance..127N":17672.80154,"Abundance..127C":18952.66204,"Abundance..128N":17203.36851,"Abundance..128C":18432.27749,"Abundance..129N":20041.75506,"Abundance..129C":18179.64524,"Abundance..130N":21961.77707,"Abundance..130C":18807.18031,"Abundance..131":20022.20603,"Ions.Score":27,"Identity.Strict":30,"Identity.Relaxed":23,"Expectation.Value":0.02559,"Percolator.q.Value":0,"Percolator.PEP":2.101e-06}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (J2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[R].vVDLMAHMASk.[E]","Modifications":"N-Term(TMT6plex); K11(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.7895,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":556.65411,"MH...Da.":1667.94779,"Theo..MH...Da.":1667.94684,"DeltaM..ppm.":0.57,"Deltam.z..Da.":0.00032,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":21.49757,"Average.Reporter.S.N":120.1,"Ion.Inject.Time..ms.":4.009,"RT..min.":146.7282,"First.Scan":75984,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture4_03.raw","File.ID":"F6","Abundance..126":827474.85893,"Abundance..127N":860990.82098,"Abundance..127C":779210.61279,"Abundance..128N":963787.34941,"Abundance..128C":840488.82478,"Abundance..129N":884950.14114,"Abundance..129C":987889.59081,"Abundance..130N":1000796.63982,"Abundance..130C":871772.50743,"Abundance..131":788255.73708,"Ions.Score":38,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.00167,"Percolator.q.Value":0,"Percolator.PEP":1.374e-06}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (G2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].fLSQPFQVAEVFTGHMGk.[L]","Modifications":"N-Term(TMT6plex); K18(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":830.45624,"MH...Da.":2489.35416,"Theo..MH...Da.":2489.35066,"DeltaM..ppm.":1.41,"Deltam.z..Da.":0.00117,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":28.04583,"Average.Reporter.S.N":40.2,"Ion.Inject.Time..ms.":22.244,"RT..min.":186.4064,"First.Scan":94942,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_02.raw","File.ID":"F9","Abundance..126":41986.35098,"Abundance..127N":34405.6643,"Abundance..127C":37844.7509,"Abundance..128N":35956.61189,"Abundance..128C":37328.5002,"Abundance..129N":33850.1546,"Abundance..129C":39330.10216,"Abundance..130N":40986.17803,"Abundance..130C":34481.385,"Abundance..131":41051.52073,"Ions.Score":49,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.00013,"Percolator.q.Value":0,"Percolator.PEP":1.816e-09}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (G2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].iPSAVGYQPTLATDMGTMQEr.[I]","Modifications":"N-Term(TMT6plex); R21(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":835.75763,"MH...Da.":2505.25833,"Theo..MH...Da.":2505.25543,"DeltaM..ppm.":1.16,"Deltam.z..Da.":0.00097,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":7.12955,"Average.Reporter.S.N":29.7,"Ion.Inject.Time..ms.":3.346,"RT..min.":162.0426,"First.Scan":81253,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_02.raw","File.ID":"F9","Abundance..126":193016.76976,"Abundance..127N":192692.91668,"Abundance..127C":177965.91704,"Abundance..128N":191844.85985,"Abundance..128C":209627.75463,"Abundance..129N":205258.85333,"Abundance..129C":196364.82361,"Abundance..130N":182983.37393,"Abundance..130C":196155.01081,"Abundance..131":219022.63297,"Ions.Score":62,"Identity.Strict":26,"Identity.Relaxed":19,"Expectation.Value":2.89234e-06,"Percolator.q.Value":0,"Percolator.PEP":3.321e-08}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (G2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[K].drLPLAVVGSNTIIEVNGk.[R]","Modifications":"N-Term(TMT6plex); R2(Label:13C(6)15N(4)); K19(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q16181","Master.Protein.Descriptions":"Septin-7 OS=Homo sapiens GN=SEPT7 PE=1 SV=2","Protein.Accessions":"Q16181","Protein.Descriptions":"Septin-7 OS=Homo sapiens GN=SEPT7 PE=1 SV=2","X..Missed.Cleavages":1,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":824.49579,"MH...Da.":2471.47281,"Theo..MH...Da.":2471.47165,"DeltaM..ppm.":0.47,"Deltam.z..Da.":0.00039,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":15.45768,"Average.Reporter.S.N":24.3,"Ion.Inject.Time..ms.":10.299,"RT..min.":157.734,"First.Scan":78758,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_02.raw","File.ID":"F9","Abundance..126":23716.34824,"Abundance..127N":22191.69542,"Abundance..127C":19810.00631,"Abundance..128N":17500.22004,"Abundance..128C":23073.93611,"Abundance..129N":23380.73743,"Abundance..129C":23038.14764,"Abundance..130N":24500.50913,"Abundance..130C":26575.10507,"Abundance..131":31940.79437,"Ions.Score":56,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":0.00002,"Percolator.q.Value":0,"Percolator.PEP":1.644e-08}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (G2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].iPSAVGYQPTLATDmGTMQEr.[I]","Modifications":"N-Term(TMT6plex); M15(Oxidation); R21(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.2308,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":841.0882,"MH...Da.":2521.25003,"Theo..MH...Da.":2521.25034,"DeltaM..ppm.":-0.12,"Deltam.z..Da.":-0.0001,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":11.29273,"Average.Reporter.S.N":10.9,"Ion.Inject.Time..ms.":6.894,"RT..min.":148.3686,"First.Scan":73389,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_02.raw","File.ID":"F9","Abundance..126":19722.17507,"Abundance..127N":19023.1499,"Abundance..127C":19735.58021,"Abundance..128N":14501.43671,"Abundance..128C":12838.69918,"Abundance..129N":16338.56716,"Abundance..129C":12363.71803,"Abundance..130N":16347.59073,"Abundance..130C":21960.1083,"Abundance..131":19017.40661,"Ions.Score":52,"Identity.Strict":25,"Identity.Relaxed":18,"Expectation.Value":0.00002,"Percolator.q.Value":0,"Percolator.PEP":8.588e-07}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (G2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].lVINGNPITIFQErDPSk.[I]","Modifications":"N-Term(TMT6plex); R14(Label:13C(6)15N(4)); K18(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":1,"Charge":3,"DeltaScore":0.8696,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":839.82452,"MH...Da.":2517.45902,"Theo..MH...Da.":2517.456,"DeltaM..ppm.":1.2,"Deltam.z..Da.":0.00101,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":0.56875,"Average.Reporter.S.N":34.2,"Ion.Inject.Time..ms.":1.724,"RT..min.":165.8185,"First.Scan":83429,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_02.raw","File.ID":"F9","Abundance..126":420662.33603,"Abundance..127N":369339.08035,"Abundance..127C":402333.21439,"Abundance..128N":467003.7552,"Abundance..128C":417958.28948,"Abundance..129N":460349.18967,"Abundance..129C":504422.39717,"Abundance..130N":406052.34762,"Abundance..130C":388757.3869,"Abundance..131":449173.40269,"Ions.Score":46,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.00022,"Percolator.q.Value":0,"Percolator.PEP":9.117e-06}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (G2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].aDTLTPEEcQQFkk.[Q]","Modifications":"N-Term(TMT6plex); C9(Carbamidomethyl); K13(Label); K14(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q16181","Master.Protein.Descriptions":"Septin-7 OS=Homo sapiens GN=SEPT7 PE=1 SV=2","Protein.Accessions":"Q16181","Protein.Descriptions":"Septin-7 OS=Homo sapiens GN=SEPT7 PE=1 SV=2","X..Missed.Cleavages":1,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":800.11401,"MH...Da.":2398.32749,"Theo..MH...Da.":2398.32261,"DeltaM..ppm.":2.04,"Deltam.z..Da.":0.00163,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":0,"Average.Reporter.S.N":12.3,"Ion.Inject.Time..ms.":30.9,"RT..min.":109.8453,"First.Scan":51868,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_02.raw","File.ID":"F9","Abundance..126":8235.16677,"Abundance..127N":10220.39785,"Abundance..127C":8303.96337,"Abundance..128N":6871.53531,"Abundance..128C":10822.10404,"Abundance..129N":7568.61953,"Abundance..129C":8040.33751,"Abundance..130N":9850.35082,"Abundance..130C":9425.79215,"Abundance..131":10897.41604,"Ions.Score":22,"Identity.Strict":30,"Identity.Relaxed":23,"Expectation.Value":0.07251,"Percolator.q.Value":0,"Percolator.PEP":0.00001}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (G2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].fGQAATMEGIGAIGGTPPAFNr.[A]","Modifications":"N-Term(TMT6plex); R22(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q15233","Master.Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","Protein.Accessions":"Q15233","Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":801.41791,"MH...Da.":2402.23917,"Theo..MH...Da.":2402.23635,"DeltaM..ppm.":1.17,"Deltam.z..Da.":0.00094,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":6.70891,"Average.Reporter.S.N":25.1,"Ion.Inject.Time..ms.":2.678,"RT..min.":169.3607,"First.Scan":85436,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_02.raw","File.ID":"F9","Abundance..126":81511.18703,"Abundance..127N":81714.25672,"Abundance..127C":73621.38065,"Abundance..128N":94327.48915,"Abundance..128C":78418.30943,"Abundance..129N":75403.02952,"Abundance..129C":79721.30929,"Abundance..130N":76044.19252,"Abundance..130C":86172.6317,"Abundance..131":76536.25584,"Ions.Score":46,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":0.00018,"Percolator.q.Value":0,"Percolator.PEP":0.00009}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (G2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].lFVGNLPPDITEEEMr.[K]","Modifications":"N-Term(TMT6plex); R16(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q15233","Master.Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","Protein.Accessions":"Q15233","Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":1050.05054,"MH...Da.":2099.0938,"Theo..MH...Da.":2099.09198,"DeltaM..ppm.":0.87,"Deltam.z..Da.":0.00091,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":0,"Average.Reporter.S.N":26.2,"Ion.Inject.Time..ms.":26.45,"RT..min.":177.4319,"First.Scan":89992,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_02.raw","File.ID":"F9","Abundance..126":9666.64685,"Abundance..127N":9617.57263,"Abundance..127C":10937.63012,"Abundance..128N":9993.04595,"Abundance..128C":8545.89427,"Abundance..129N":12570.77857,"Abundance..129C":12551.55137,"Abundance..130N":10174.51535,"Abundance..130C":9941.27718,"Abundance..131":11278.72249,"Ions.Score":55,"Identity.Strict":27,"Identity.Relaxed":20,"Expectation.Value":0.00002,"Percolator.q.Value":0,"Percolator.PEP":3.371e-07}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (G2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].gTGGVDTAAVGGVFDVSNADr.[L]","Modifications":"N-Term(TMT6plex); R21(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P12277","Master.Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","Protein.Accessions":"P12277","Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":735.37256,"MH...Da.":2204.10312,"Theo..MH...Da.":2204.10202,"DeltaM..ppm.":0.5,"Deltam.z..Da.":0.00037,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":42.15272,"Average.Reporter.S.N":20.8,"Ion.Inject.Time..ms.":9.382,"RT..min.":149.1662,"First.Scan":73857,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_02.raw","File.ID":"F9","Abundance..126":29637.84712,"Abundance..127N":30991.24614,"Abundance..127C":28445.91494,"Abundance..128N":32822.90245,"Abundance..128C":30043.12906,"Abundance..129N":42581.62225,"Abundance..129C":33903.09666,"Abundance..130N":32991.70828,"Abundance..130C":32925.39798,"Abundance..131":28955.14566,"Ions.Score":65,"Identity.Strict":25,"Identity.Relaxed":18,"Expectation.Value":1.23157e-06,"Percolator.q.Value":0,"Percolator.PEP":2.648e-09}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (G2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].iMDPNIVGSEHYDVAr.[G]","Modifications":"N-Term(TMT6plex); R16(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":685.68561,"MH...Da.":2055.04227,"Theo..MH...Da.":2055.04061,"DeltaM..ppm.":0.81,"Deltam.z..Da.":0.00055,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":28.71026,"Average.Reporter.S.N":21.9,"Ion.Inject.Time..ms.":4.224,"RT..min.":130.4654,"First.Scan":63313,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_02.raw","File.ID":"F9","Abundance..126":55570.71367,"Abundance..127N":66419.33477,"Abundance..127C":59068.53153,"Abundance..128N":71995.227,"Abundance..128C":57411.27933,"Abundance..129N":53092.91217,"Abundance..129C":77318.21954,"Abundance..130N":62426.49116,"Abundance..130C":66720.90312,"Abundance..131":64282.64637,"Ions.Score":38,"Identity.Strict":26,"Identity.Relaxed":19,"Expectation.Value":0.00068,"Percolator.q.Value":0,"Percolator.PEP":1.559e-06}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (G2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].fcTGLTQIETLFk.[S]","Modifications":"N-Term(TMT6plex); C2(Carbamidomethyl); K13(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P12277","Master.Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","Protein.Accessions":"P12277","Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":1012.57062,"MH...Da.":2024.13396,"Theo..MH...Da.":2024.1382,"DeltaM..ppm.":-2.1,"Deltam.z..Da.":-0.00212,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":36.04862,"Average.Reporter.S.N":17.3,"Ion.Inject.Time..ms.":25.663,"RT..min.":195.7886,"First.Scan":99903,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_02.raw","File.ID":"F9","Abundance..126":15731.6499,"Abundance..127N":22546.86296,"Abundance..127C":15903.76747,"Abundance..128N":13723.28041,"Abundance..128C":16512.43023,"Abundance..129N":16271.91627,"Abundance..129C":12767.05851,"Abundance..130N":17467.74498,"Abundance..130C":13081.15516,"Abundance..131":14883.89096,"Ions.Score":20,"Identity.Strict":30,"Identity.Relaxed":23,"Expectation.Value":0.10424,"Percolator.q.Value":0,"Percolator.PEP":4.146e-06}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (B2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].aSEGPAFFPGr.[C]","Modifications":"N-Term(TMT6plex); R11(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q9NSD9","Master.Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","Protein.Accessions":"Q9NSD9","Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":687.86633,"MH...Da.":1374.72539,"Theo..MH...Da.":1374.72428,"DeltaM..ppm.":0.8,"Deltam.z..Da.":0.00055,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":32.43635,"Average.Reporter.S.N":13.4,"Ion.Inject.Time..ms.":3.549,"RT..min.":116.681,"First.Scan":59307,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_01.raw","File.ID":"F14","Abundance..126":45748.33442,"Abundance..127N":40148.65982,"Abundance..127C":37409.88898,"Abundance..128N":46544.28149,"Abundance..128C":39370.06398,"Abundance..129N":35574.2851,"Abundance..129C":45279.42368,"Abundance..130N":40666.0942,"Abundance..130C":44144.00722,"Abundance..131":45437.19338,"Ions.Score":68,"Identity.Strict":26,"Identity.Relaxed":19,"Expectation.Value":6.89429e-07,"Percolator.q.Value":0,"Percolator.PEP":0.00011}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (B2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[K].vLDSGAPIk.[I]","Modifications":"N-Term(TMT6plex); K9(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":0.7833,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":683.43402,"MH...Da.":1365.86077,"Theo..MH...Da.":1365.85972,"DeltaM..ppm.":0.76,"Deltam.z..Da.":0.00052,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":4.24591,"Average.Reporter.S.N":122.6,"Ion.Inject.Time..ms.":4.441,"RT..min.":117.0149,"First.Scan":59502,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_01.raw","File.ID":"F14","Abundance..126":595343.48685,"Abundance..127N":585865.35335,"Abundance..127C":614244.36344,"Abundance..128N":589842.0946,"Abundance..128C":570887.02327,"Abundance..129N":574868.37488,"Abundance..129C":581840.3182,"Abundance..130N":596688.51845,"Abundance..130C":554526.39806,"Abundance..131":558101.42731,"Ions.Score":60,"Identity.Strict":27,"Identity.Relaxed":20,"Expectation.Value":5.6031e-06,"Percolator.q.Value":0,"Percolator.PEP":0.00005}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (N2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].sLQDIIAILGmDELSEEDk.[L]","Modifications":"N-Term(TMT6plex); M11(Oxidation); K19(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":867.80017,"MH...Da.":2601.38596,"Theo..MH...Da.":2601.38247,"DeltaM..ppm.":1.34,"Deltam.z..Da.":0.00116,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":6.69216,"Average.Reporter.S.N":62.6,"Ion.Inject.Time..ms.":18.983,"RT..min.":221.0872,"First.Scan":114621,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_03.raw","File.ID":"F2","Abundance..126":58771.406,"Abundance..127N":49590.66236,"Abundance..127C":56018.54443,"Abundance..128N":58764.1916,"Abundance..128C":53646.37015,"Abundance..129N":58281.1895,"Abundance..129C":62891.71563,"Abundance..130N":47891.36555,"Abundance..130C":57194.68626,"Abundance..131":46991.7967,"Ions.Score":73,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":5.29448e-07,"Percolator.q.Value":0,"Percolator.PEP":7.286e-09}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (N4)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].iISNAScTTNcLAPLAk.[V]","Modifications":"N-Term(TMT6plex); C7(Carbamidomethyl); C11(Carbamidomethyl); K17(TMT6plex)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.8214,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":764.75439,"MH...Da.":2292.24863,"Theo..MH...Da.":2292.24559,"DeltaM..ppm.":1.32,"Deltam.z..Da.":0.00101,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":18.91111,"Average.Reporter.S.N":38.8,"Ion.Inject.Time..ms.":4.279,"RT..min.":149.3887,"First.Scan":75440,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_03.raw","File.ID":"F2","Abundance..126":179784.84399,"Abundance..127N":175738.37155,"Abundance..127C":189826.53624,"Abundance..128N":179898.40367,"Abundance..128C":186013.45163,"Abundance..129N":181447.92648,"Abundance..129C":167708.44148,"Abundance..130N":184883.31238,"Abundance..130C":168624.74563,"Abundance..131":165194.36132,"Ions.Score":56,"Identity.Strict":30,"Identity.Relaxed":23,"Expectation.Value":0.00002,"Percolator.q.Value":0,"Percolator.PEP":0.00134}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (N4)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[R].gALQNIIPASTGAAk.[A]","Modifications":"N-Term(TMT6plex); K15(TMT6plex)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":935.56244,"MH...Da.":1870.1176,"Theo..MH...Da.":1870.11622,"DeltaM..ppm.":0.74,"Deltam.z..Da.":0.00069,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":41.77663,"Average.Reporter.S.N":17,"Ion.Inject.Time..ms.":50,"RT..min.":141.6517,"First.Scan":70987,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_03.raw","File.ID":"F2","Abundance..126":6388.63905,"Abundance..127N":7820.50401,"Abundance..127C":8418.52767,"Abundance..128N":6254.21414,"Abundance..128C":8982.99157,"Abundance..129N":7760.85488,"Abundance..129C":9645.47778,"Abundance..130N":7805.36381,"Abundance..130C":7192.54962,"Abundance..131":7599.76629,"Ions.Score":88,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":1.23023e-08,"Percolator.q.Value":0,"Percolator.PEP":0.00006}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (O2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[R].iLEQQNSSr.[T]","Modifications":"N-Term(TMT6plex); R9(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q16181","Master.Protein.Descriptions":"Septin-7 OS=Homo sapiens GN=SEPT7 PE=1 SV=2","Protein.Accessions":"Q16181","Protein.Descriptions":"Septin-7 OS=Homo sapiens GN=SEPT7 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":0.5,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":657.3667,"MH...Da.":1313.72612,"Theo..MH...Da.":1313.72501,"DeltaM..ppm.":0.84,"Deltam.z..Da.":0.00055,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":25.26185,"Average.Reporter.S.N":6.7,"Ion.Inject.Time..ms.":50,"RT..min.":59.3105,"First.Scan":25407,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_03.raw","File.ID":"F1","Abundance..126":2296.59908,"Abundance..127N":2095.26588,"Abundance..127C":3058.0655,"Abundance..128N":2878.34122,"Abundance..128C":2614.56727,"Abundance..129N":1849.64819,"Abundance..129C":2980.48053,"Abundance..130N":1736.51609,"Abundance..130C":2184.4201,"Abundance..131":1965.42931,"Ions.Score":40,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.00086,"Percolator.q.Value":0,"Percolator.PEP":0.00018}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (O2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[R].vLLLSHLAr.[M]","Modifications":"N-Term(TMT6plex); R9(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q9UGP8","Master.Protein.Descriptions":"Translocation protein SEC63 homolog OS=Homo sapiens GN=SEC63 PE=1 SV=2","Protein.Accessions":"Q9UGP8","Protein.Descriptions":"Translocation protein SEC63 homolog OS=Homo sapiens GN=SEC63 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.6857,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":420.94598,"MH...Da.":1260.8234,"Theo..MH...Da.":1260.82288,"DeltaM..ppm.":0.41,"Deltam.z..Da.":0.00017,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":16.35469,"Average.Reporter.S.N":75.4,"Ion.Inject.Time..ms.":19.412,"RT..min.":122.1997,"First.Scan":61371,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_03.raw","File.ID":"F1","Abundance..126":38330.04776,"Abundance..127N":35998.7172,"Abundance..127C":40780.46982,"Abundance..128N":40452.20514,"Abundance..128C":40122.61674,"Abundance..129N":38811.56201,"Abundance..129C":37253.03842,"Abundance..130N":42186.82803,"Abundance..130C":40109.84847,"Abundance..131":41253.53273,"Ions.Score":35,"Identity.Strict":23,"Identity.Relaxed":16,"Expectation.Value":0.00072,"Percolator.q.Value":0,"Percolator.PEP":0.00022}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (B2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].lVEALcAAGHr.[A]","Modifications":"N-Term(TMT6plex); C6(Carbamidomethyl); R11(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P23919","Master.Protein.Descriptions":"Thymidylate kinase OS=Homo sapiens GN=DTYMK PE=1 SV=4","Protein.Accessions":"P23919","Protein.Descriptions":"Thymidylate kinase OS=Homo sapiens GN=DTYMK PE=1 SV=4","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":479.2681,"MH...Da.":1435.78974,"Theo..MH...Da.":1435.79165,"DeltaM..ppm.":-1.33,"Deltam.z..Da.":-0.00064,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":50.50306,"Average.Reporter.S.N":43.8,"Ion.Inject.Time..ms.":15.386,"RT..min.":104.4118,"First.Scan":52235,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_01.raw","File.ID":"F14","Abundance..126":45385.46492,"Abundance..127N":45462.78569,"Abundance..127C":46232.51752,"Abundance..128N":44556.08154,"Abundance..128C":46084.98785,"Abundance..129N":42297.59268,"Abundance..129C":52784.88669,"Abundance..130N":48495.12725,"Abundance..130C":49099.03023,"Abundance..131":43421.60422,"Ions.Score":26,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":0.02117,"Percolator.q.Value":0,"Percolator.PEP":0.00013}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (B2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].vETELQGVcDTVLGLLDSHLIk.[E]","Modifications":"N-Term(TMT6plex); C9(Carbamidomethyl); K22(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P31947","Master.Protein.Descriptions":"14-3-3 protein sigma OS=Homo sapiens GN=SFN PE=1 SV=1","Protein.Accessions":"P31947","Protein.Descriptions":"14-3-3 protein sigma OS=Homo sapiens GN=SFN PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.9894,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":969.21283,"MH...Da.":2905.62394,"Theo..MH...Da.":2905.62001,"DeltaM..ppm.":1.35,"Deltam.z..Da.":0.00131,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":3.16716,"Average.Reporter.S.N":28.2,"Ion.Inject.Time..ms.":8.598,"RT..min.":221.4908,"First.Scan":117820,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_01.raw","File.ID":"F14","Abundance..126":48041.46279,"Abundance..127N":48567.89446,"Abundance..127C":39391.54635,"Abundance..128N":55644.3419,"Abundance..128C":47925.53974,"Abundance..129N":51089.37956,"Abundance..129C":55980.59467,"Abundance..130N":44748.83178,"Abundance..130C":58160.64773,"Abundance..131":45398.29246,"Ions.Score":94,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":3.81779e-09,"Percolator.q.Value":0,"Percolator.PEP":6.957e-14}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (B2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].nLPQYVSNELLEEAFSVFGQVEr.[A]","Modifications":"N-Term(TMT6plex); R23(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q15233","Master.Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","Protein.Accessions":"Q15233","Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":969.83789,"MH...Da.":2907.49912,"Theo..MH...Da.":2907.49652,"DeltaM..ppm.":0.89,"Deltam.z..Da.":0.00087,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":2.32838,"Average.Reporter.S.N":1.4,"Ion.Inject.Time..ms.":50,"RT..min.":246.3884,"First.Scan":129035,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_01.raw","File.ID":"F14","Abundance..128N":934.63415,"Abundance..129N":1065.2057,"Abundance..129C":1076.83681,"Abundance..130N":472.32301,"Abundance..130C":702.23653,"Abundance..131":522.85277,"Ions.Score":70,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":7.21953e-07,"Percolator.q.Value":0,"Percolator.PEP":1.908e-09}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (N2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[K].lAVEALSSLDGDLAGr.[Y]","Modifications":"N-Term(TMT6plex); R16(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P12277","Master.Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","Protein.Accessions":"P12277","Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.9077,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":609.34192,"MH...Da.":1826.0112,"Theo..MH...Da.":1826.00963,"DeltaM..ppm.":0.86,"Deltam.z..Da.":0.00053,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":5.61759,"Average.Reporter.S.N":33.5,"Ion.Inject.Time..ms.":1.72,"RT..min.":181.588,"First.Scan":93967,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_03.raw","File.ID":"F2","Abundance..126":376672.50344,"Abundance..127N":387137.65518,"Abundance..127C":335521.72723,"Abundance..128N":309526.07112,"Abundance..128C":364219.33849,"Abundance..129N":375517.48829,"Abundance..129C":297724.84703,"Abundance..130N":306736.32519,"Abundance..130C":462937.40543,"Abundance..131":370642.30989,"Ions.Score":65,"Identity.Strict":30,"Identity.Relaxed":23,"Expectation.Value":3.1304e-06,"Percolator.q.Value":0,"Percolator.PEP":2.249e-07}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (B4)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[R].gALQNIIPASTGAAk.[A]","Modifications":"N-Term(TMT6plex); K15(TMT6plex)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":0.9385,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":935.56366,"MH...Da.":1870.12004,"Theo..MH...Da.":1870.11622,"DeltaM..ppm.":2.05,"Deltam.z..Da.":0.00191,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":0,"Average.Reporter.S.N":21.4,"Ion.Inject.Time..ms.":50,"RT..min.":142.6495,"First.Scan":74216,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_01.raw","File.ID":"F14","Abundance..126":7773.56046,"Abundance..127N":10856.378,"Abundance..127C":9448.73811,"Abundance..128N":10392.97352,"Abundance..128C":8878.49119,"Abundance..129N":10537.90769,"Abundance..129C":11378.69134,"Abundance..130N":9600.64527,"Abundance..130C":8117.90515,"Abundance..131":9643.14515,"Ions.Score":65,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":2.51384e-06,"Percolator.q.Value":0,"Percolator.PEP":0.00029}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (B4)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].vPTANVSVVDLTcR.[L]","Modifications":"N-Term(TMT6plex); C13(Carbamidomethyl)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":0.9608,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":880.48175,"MH...Da.":1759.95622,"Theo..MH...Da.":1759.95739,"DeltaM..ppm.":-0.66,"Deltam.z..Da.":-0.00058,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":29.70587,"Average.Reporter.S.N":13.6,"Ion.Inject.Time..ms.":8.872,"RT..min.":147.5206,"First.Scan":77064,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_01.raw","File.ID":"F14","Abundance..126":26665.12417,"Abundance..127N":19478.64047,"Abundance..127C":19280.06321,"Abundance..128N":25417.62579,"Abundance..128C":17560.22937,"Abundance..129N":13798.81457,"Abundance..129C":17766.43027,"Abundance..130N":23685.89569,"Abundance..130C":23699.01948,"Abundance..131":22660.55885,"Ions.Score":51,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.00008,"Percolator.q.Value":0,"Percolator.PEP":0.00201}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (B2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].aDIIHAcDIVEDAAIAYGYNNIQMTLPk.[T]","Modifications":"N-Term(TMT6plex); C7(Carbamidomethyl); K28(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q9NSD9","Master.Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","Protein.Accessions":"Q9NSD9","Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","X..Missed.Cleavages":0,"Charge":4,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":897.2193,"MH...Da.":3585.85537,"Theo..MH...Da.":3585.8577,"DeltaM..ppm.":-0.65,"Deltam.z..Da.":-0.00058,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":22.02045,"Average.Reporter.S.N":35.6,"Ion.Inject.Time..ms.":15.551,"RT..min.":206.1666,"First.Scan":110266,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_01.raw","File.ID":"F14","Abundance..126":55511.5455,"Abundance..127N":49934.64818,"Abundance..127C":52520.32542,"Abundance..128N":49715.40976,"Abundance..128C":56649.02077,"Abundance..129N":56654.34581,"Abundance..129C":61961.3297,"Abundance..130N":48053.73096,"Abundance..130C":50762.395,"Abundance..131":56378.19766,"Ions.Score":14,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":0.30554,"Percolator.q.Value":0,"Percolator.PEP":0.00009}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (B2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].gFQQILAGEYDHLPEQAFYMVGPIEEAVAk.[A]","Modifications":"N-Term(TMT6plex); K30(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":1272.99915,"MH...Da.":3816.98288,"Theo..MH...Da.":3816.98025,"DeltaM..ppm.":0.69,"Deltam.z..Da.":0.00088,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":4.38537,"Average.Reporter.S.N":26.2,"Ion.Inject.Time..ms.":40.165,"RT..min.":213.2166,"First.Scan":113826,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_01.raw","File.ID":"F14","Abundance..126":23686.39534,"Abundance..127N":16872.05586,"Abundance..127C":19157.2914,"Abundance..128N":20358.36461,"Abundance..128C":21075.39319,"Abundance..129N":22737.95245,"Abundance..129C":18663.34145,"Abundance..130N":22239.13526,"Abundance..130C":21072.21023,"Abundance..131":23289.10536,"Ions.Score":48,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":0.00013,"Percolator.q.Value":0,"Percolator.PEP":7.917e-08}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (B2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].lGFSEVELVQMVVDGVk.[L]","Modifications":"N-Term(TMT6plex); K17(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P12277","Master.Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","Protein.Accessions":"P12277","Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.9643,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":772.4447,"MH...Da.":2315.31955,"Theo..MH...Da.":2315.31763,"DeltaM..ppm.":0.83,"Deltam.z..Da.":0.00064,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":2.29361,"Average.Reporter.S.N":25.4,"Ion.Inject.Time..ms.":14.101,"RT..min.":224.5872,"First.Scan":119266,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_01.raw","File.ID":"F14","Abundance..126":28884.46537,"Abundance..127N":35562.75516,"Abundance..127C":28092.8186,"Abundance..128N":26191.72908,"Abundance..128C":30220.85705,"Abundance..129N":29956.55575,"Abundance..129C":29430.16501,"Abundance..130N":37116.23039,"Abundance..130C":30625.53023,"Abundance..131":33949.96788,"Ions.Score":28,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.01448,"Percolator.q.Value":0,"Percolator.PEP":0.00003}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (B2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[R].dQEGQDVLLFIDNIFr.[F]","Modifications":"N-Term(TMT6plex); R16(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.9655,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":721.05255,"MH...Da.":2161.1431,"Theo..MH...Da.":2161.13662,"DeltaM..ppm.":3,"Deltam.z..Da.":0.00216,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":0,"Average.Reporter.S.N":27.9,"Ion.Inject.Time..ms.":50,"RT..min.":233.3986,"First.Scan":123306,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_01.raw","File.ID":"F14","Abundance..126":8372.80807,"Abundance..127N":7736.18027,"Abundance..127C":9912.93844,"Abundance..128N":9303.26028,"Abundance..128C":10174.73397,"Abundance..129N":9870.81386,"Abundance..129C":12768.86489,"Abundance..130N":8682.22339,"Abundance..130C":10361.86145,"Abundance..131":8914.86512,"Ions.Score":29,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.01019,"Percolator.q.Value":0,"Percolator.PEP":0.00003}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (N2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[R].vALTGLTVAEYFr.[D]","Modifications":"N-Term(TMT6plex); R13(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":839.98462,"MH...Da.":1678.96196,"Theo..MH...Da.":1678.96049,"DeltaM..ppm.":0.88,"Deltam.z..Da.":0.00073,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":21.16801,"Average.Reporter.S.N":21.2,"Ion.Inject.Time..ms.":7.503,"RT..min.":181.6348,"First.Scan":93995,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_03.raw","File.ID":"F2","Abundance..126":147049.89194,"Abundance..127N":120961.96274,"Abundance..127C":147745.61898,"Abundance..128N":134867.78702,"Abundance..128C":115646.91923,"Abundance..129N":111288.47088,"Abundance..129C":143069.80743,"Abundance..130N":102296.12912,"Abundance..130C":114084.6524,"Abundance..131":137433.89473,"Ions.Score":64,"Identity.Strict":30,"Identity.Relaxed":23,"Expectation.Value":4.46998e-06,"Percolator.q.Value":0,"Percolator.PEP":2.949e-06}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (N2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].mGQMAMGGAMGINNr.[G]","Modifications":"N-Term(TMT6plex); R15(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q15233","Master.Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","Protein.Accessions":"Q15233","Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":889.42432,"MH...Da.":1777.84136,"Theo..MH...Da.":1777.84067,"DeltaM..ppm.":0.39,"Deltam.z..Da.":0.00034,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":0,"Average.Reporter.S.N":6.1,"Ion.Inject.Time..ms.":50,"RT..min.":126.2105,"First.Scan":62285,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_03.raw","File.ID":"F2","Abundance..126":1604.1445,"Abundance..127N":2275.70962,"Abundance..127C":1548.43656,"Abundance..128N":2491.86925,"Abundance..128C":1546.79563,"Abundance..129N":2299.86237,"Abundance..129C":2476.5324,"Abundance..130N":1475.92519,"Abundance..130C":2271.16576,"Abundance..131":2549.9432,"Ions.Score":68,"Identity.Strict":21,"Identity.Relaxed":14,"Expectation.Value":2.05562e-07,"Percolator.q.Value":0,"Percolator.PEP":8.668e-07}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (N2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].vPTANVSVVDLTcr.[L]","Modifications":"N-Term(TMT6plex); C13(Carbamidomethyl); R14(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":885.48749,"MH...Da.":1769.9677,"Theo..MH...Da.":1769.96565,"DeltaM..ppm.":1.16,"Deltam.z..Da.":0.00102,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":21.91645,"Average.Reporter.S.N":8.6,"Ion.Inject.Time..ms.":3.684,"RT..min.":146.4626,"First.Scan":73745,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_03.raw","File.ID":"F2","Abundance..126":41016.15158,"Abundance..127N":50364.0579,"Abundance..127C":55305.31246,"Abundance..128N":35349.31355,"Abundance..128C":45981.47022,"Abundance..129N":48796.92711,"Abundance..129C":66336.78452,"Abundance..130N":55470.39882,"Abundance..130C":58644.90826,"Abundance..131":44418.00121,"Ions.Score":57,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.00002,"Percolator.q.Value":0,"Percolator.PEP":0.00002}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (B4)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].lISWYDNEFGYSNR.[V]","Modifications":"N-Term(TMT6plex)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":996.98761,"MH...Da.":1992.96794,"Theo..MH...Da.":1992.96531,"DeltaM..ppm.":1.32,"Deltam.z..Da.":0.00132,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":0,"Average.Reporter.S.N":18.2,"Ion.Inject.Time..ms.":11.047,"RT..min.":178.1921,"First.Scan":94879,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_01.raw","File.ID":"F14","Abundance..126":20714.87061,"Abundance..127N":19080.70081,"Abundance..127C":18887.18657,"Abundance..128N":16924.22028,"Abundance..128C":20258.6125,"Abundance..129N":20376.58073,"Abundance..129C":19535.50559,"Abundance..130N":19074.6785,"Abundance..130C":18918.20862,"Abundance..131":21461.16512,"Ions.Score":77,"Identity.Strict":24,"Identity.Relaxed":17,"Expectation.Value":5.91319e-08,"Percolator.q.Value":0,"Percolator.PEP":0.00023}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (N2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].fTQAGSEVSALLGr.[I]","Modifications":"N-Term(TMT6plex); R14(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.9651,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":558.98004,"MH...Da.":1674.92557,"Theo..MH...Da.":1674.92517,"DeltaM..ppm.":0.24,"Deltam.z..Da.":0.00013,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":5.83353,"Average.Reporter.S.N":33.2,"Ion.Inject.Time..ms.":6.586,"RT..min.":162.044,"First.Scan":82803,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_03.raw","File.ID":"F2","Abundance..126":163974.1116,"Abundance..127N":175753.35508,"Abundance..127C":155573.59949,"Abundance..128N":132744.9355,"Abundance..128C":143201.84846,"Abundance..129N":151906.42776,"Abundance..129C":167235.49863,"Abundance..130N":156527.35268,"Abundance..130C":143900.91302,"Abundance..131":173106.98177,"Ions.Score":86,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":2.53671e-08,"Percolator.q.Value":0,"Percolator.PEP":3.33e-08}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (N2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].aHGGYSVFAGVGEr.[T]","Modifications":"N-Term(TMT6plex); R14(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":549.28925,"MH...Da.":1645.85318,"Theo..MH...Da.":1645.85234,"DeltaM..ppm.":0.51,"Deltam.z..Da.":0.00028,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":37.3567,"Average.Reporter.S.N":30.6,"Ion.Inject.Time..ms.":7.89,"RT..min.":87.4717,"First.Scan":40382,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_03.raw","File.ID":"F2","Abundance..126":133217.06085,"Abundance..127N":107296.62839,"Abundance..127C":105995.19986,"Abundance..128N":157272.31226,"Abundance..128C":105739.40826,"Abundance..129N":127911.3408,"Abundance..129C":150348.44227,"Abundance..130N":139159.43608,"Abundance..130C":114983.88624,"Abundance..131":131957.37334,"Ions.Score":47,"Identity.Strict":27,"Identity.Relaxed":20,"Expectation.Value":0.00011,"Percolator.q.Value":0,"Percolator.PEP":8.349e-06}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (B2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].iMNVIGEPIDErGPIk.[T]","Modifications":"N-Term(TMT6plex); R12(Label:13C(6)15N(4)); K16(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":1,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":753.10962,"MH...Da.":2257.3143,"Theo..MH...Da.":2257.31091,"DeltaM..ppm.":1.5,"Deltam.z..Da.":0.00113,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":54.0131,"Average.Reporter.S.N":29.9,"Ion.Inject.Time..ms.":2.629,"RT..min.":152.7292,"First.Scan":80086,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_01.raw","File.ID":"F14","Abundance..126":236142.21813,"Abundance..127N":213069.51398,"Abundance..127C":223556.91369,"Abundance..128N":213497.63216,"Abundance..128C":235748.05227,"Abundance..129N":211294.65303,"Abundance..129C":272948.4536,"Abundance..130N":273580.10074,"Abundance..130C":199954.85603,"Abundance..131":175653.84507,"Ions.Score":18,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.14956,"Percolator.q.Value":0,"Percolator.PEP":0.00009}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (B2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].kNEPPLTcPYSLk.[A]","Modifications":"N-Term(TMT6plex); K1(Label); C8(Carbamidomethyl); K13(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q9UGP8","Master.Protein.Descriptions":"Translocation protein SEC63 homolog OS=Homo sapiens GN=SEC63 PE=1 SV=2","Protein.Accessions":"Q9UGP8","Protein.Descriptions":"Translocation protein SEC63 homolog OS=Homo sapiens GN=SEC63 PE=1 SV=2","X..Missed.Cleavages":1,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":750.77472,"MH...Da.":2250.3096,"Theo..MH...Da.":2250.31058,"DeltaM..ppm.":-0.44,"Deltam.z..Da.":-0.00033,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":25.23501,"Average.Reporter.S.N":29.4,"Ion.Inject.Time..ms.":38.506,"RT..min.":118.3983,"First.Scan":60288,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_01.raw","File.ID":"F14","Abundance..126":25477.82413,"Abundance..127N":23728.22887,"Abundance..127C":30933.62053,"Abundance..128N":30946.65173,"Abundance..128C":28333.63515,"Abundance..129N":29055.43163,"Abundance..129C":29889.68171,"Abundance..130N":19320.84099,"Abundance..130C":28164.79215,"Abundance..131":27183.05099,"Ions.Score":26,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.02245,"Percolator.q.Value":0,"Percolator.PEP":2.283e-06}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (B2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[K].nLEGYVGFANLPNQVYr.[K]","Modifications":"N-Term(TMT6plex); R17(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q16181","Master.Protein.Descriptions":"Septin-7 OS=Homo sapiens GN=SEPT7 PE=1 SV=2","Protein.Accessions":"Q16181","Protein.Descriptions":"Septin-7 OS=Homo sapiens GN=SEPT7 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":731.72327,"MH...Da.":2193.15525,"Theo..MH...Da.":2193.15294,"DeltaM..ppm.":1.05,"Deltam.z..Da.":0.00077,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":13.19767,"Average.Reporter.S.N":13.6,"Ion.Inject.Time..ms.":3.977,"RT..min.":170.2785,"First.Scan":90325,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_01.raw","File.ID":"F14","Abundance..126":48282.48836,"Abundance..127N":64795.6537,"Abundance..127C":47177.86411,"Abundance..128N":45501.32842,"Abundance..128C":47135.32236,"Abundance..129N":45914.70747,"Abundance..129C":54560.56844,"Abundance..130N":46374.76437,"Abundance..130C":60577.08245,"Abundance..131":41142.77985,"Ions.Score":24,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":0.03245,"Percolator.q.Value":0,"Percolator.PEP":0.00002}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (B2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].nLEGYVGFANLPNQVYr.[K]","Modifications":"N-Term(TMT6plex); R17(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q16181","Master.Protein.Descriptions":"Septin-7 OS=Homo sapiens GN=SEPT7 PE=1 SV=2","Protein.Accessions":"Q16181","Protein.Descriptions":"Septin-7 OS=Homo sapiens GN=SEPT7 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":0.9691,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":1097.08069,"MH...Da.":2193.1541,"Theo..MH...Da.":2193.15294,"DeltaM..ppm.":0.53,"Deltam.z..Da.":0.00058,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":0,"Average.Reporter.S.N":16.1,"Ion.Inject.Time..ms.":38.277,"RT..min.":170.2929,"First.Scan":90336,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_01.raw","File.ID":"F14","Abundance..126":5002.98145,"Abundance..127N":5976.72913,"Abundance..127C":5158.9896,"Abundance..128N":6268.72954,"Abundance..128C":6847.33992,"Abundance..129N":6253.16681,"Abundance..129C":7770.72268,"Abundance..130N":5526.05017,"Abundance..130C":6135.25827,"Abundance..131":5631.34247,"Ions.Score":97,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":1.45995e-09,"Percolator.q.Value":0,"Percolator.PEP":4.879e-09}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (B2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].aIAELGIYPAVDPLDSTSr.[I]","Modifications":"N-Term(TMT6plex); R19(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":1114.10632,"MH...Da.":2227.20537,"Theo..MH...Da.":2227.2047,"DeltaM..ppm.":0.3,"Deltam.z..Da.":0.00034,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":100,"Average.Reporter.S.N":25.5,"Ion.Inject.Time..ms.":29.41,"RT..min.":185.1201,"First.Scan":98839,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_01.raw","File.ID":"F14","Abundance..126":11336.47887,"Abundance..127N":11411.42969,"Abundance..127C":12309.22076,"Abundance..128N":11286.80559,"Abundance..128C":11621.75791,"Abundance..129N":9547.28652,"Abundance..129C":12675.62485,"Abundance..130N":12756.92331,"Abundance..130C":12745.7946,"Abundance..131":8621.07365,"Ions.Score":12,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.53805,"Percolator.q.Value":0,"Percolator.PEP":0.00023}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (N2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].yLAEVATGDDkk.[R]","Modifications":"N-Term(TMT6plex); K11(Label); K12(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P31947","Master.Protein.Descriptions":"14-3-3 protein sigma OS=Homo sapiens GN=SFN PE=1 SV=1","Protein.Accessions":"P31947","Protein.Descriptions":"14-3-3 protein sigma OS=Homo sapiens GN=SFN PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":671.7326,"MH...Da.":2013.18326,"Theo..MH...Da.":2013.18062,"DeltaM..ppm.":1.31,"Deltam.z..Da.":0.00088,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":32.41198,"Average.Reporter.S.N":41.3,"Ion.Inject.Time..ms.":9.594,"RT..min.":111.4165,"First.Scan":53911,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_03.raw","File.ID":"F2","Abundance..126":83817.3559,"Abundance..127N":89166.71044,"Abundance..127C":80171.34727,"Abundance..128N":69828.17199,"Abundance..128C":80417.87786,"Abundance..129N":74542.56231,"Abundance..129C":84778.24144,"Abundance..130N":78480.12945,"Abundance..130C":94777.60913,"Abundance..131":65772.99456,"Ions.Score":12,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.64087,"Percolator.q.Value":0,"Percolator.PEP":0.00006}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (B2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].aDTLTPEEcQQFkk.[Q]","Modifications":"N-Term(TMT6plex); C9(Carbamidomethyl); K13(Label); K14(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q16181","Master.Protein.Descriptions":"Septin-7 OS=Homo sapiens GN=SEPT7 PE=1 SV=2","Protein.Accessions":"Q16181","Protein.Descriptions":"Septin-7 OS=Homo sapiens GN=SEPT7 PE=1 SV=2","X..Missed.Cleavages":1,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":800.11261,"MH...Da.":2398.32328,"Theo..MH...Da.":2398.32261,"DeltaM..ppm.":0.28,"Deltam.z..Da.":0.00022,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":14.85056,"Average.Reporter.S.N":17.5,"Ion.Inject.Time..ms.":18.048,"RT..min.":110.5421,"First.Scan":55765,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_01.raw","File.ID":"F14","Abundance..126":11134.8009,"Abundance..127N":12735.58332,"Abundance..127C":14095.74522,"Abundance..128N":11363.08336,"Abundance..128C":11489.11536,"Abundance..129N":10487.72908,"Abundance..129C":9790.50898,"Abundance..130N":11481.42097,"Abundance..130C":12112.16955,"Abundance..131":11590.23854,"Ions.Score":20,"Identity.Strict":30,"Identity.Relaxed":23,"Expectation.Value":0.11332,"Percolator.q.Value":0,"Percolator.PEP":9.21e-06}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (B2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].rGTGGVDTAAVGGVFDVSNADr.[L]","Modifications":"N-Term(TMT6plex); R1(Label:13C(6)15N(4)); R22(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P12277","Master.Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","Protein.Accessions":"P12277","Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":790.74225,"MH...Da.":2370.21219,"Theo..MH...Da.":2370.2114,"DeltaM..ppm.":0.33,"Deltam.z..Da.":0.00026,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":0,"Average.Reporter.S.N":32.6,"Ion.Inject.Time..ms.":5.165,"RT..min.":121.5389,"First.Scan":62093,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_01.raw","File.ID":"F14","Abundance..126":114446.49221,"Abundance..127N":101261.4452,"Abundance..127C":120630.77242,"Abundance..128N":130317.47715,"Abundance..128C":101931.3491,"Abundance..129N":116370.09557,"Abundance..129C":124489.76975,"Abundance..130N":119610.6229,"Abundance..130C":106684.88757,"Abundance..131":104544.49633,"Ions.Score":92,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":4.3502e-09,"Percolator.q.Value":0,"Percolator.PEP":2.735e-10}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (B2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[R].vPTANVSVVDLTcrLEk.[P]","Modifications":"N-Term(TMT6plex); C13(Carbamidomethyl); R14(Label:13C(6)15N(4)); K17(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":1,"Charge":3,"DeltaScore":0.9333,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":793.12695,"MH...Da.":2377.36631,"Theo..MH...Da.":2377.36441,"DeltaM..ppm.":0.8,"Deltam.z..Da.":0.00063,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":7.27698,"Average.Reporter.S.N":60.9,"Ion.Inject.Time..ms.":1.834,"RT..min.":155.1611,"First.Scan":81510,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_01.raw","File.ID":"F14","Abundance..126":289912.18965,"Abundance..127N":295202.01796,"Abundance..127C":244442.79526,"Abundance..128N":291567.9833,"Abundance..128C":290761.06585,"Abundance..129N":270004.3941,"Abundance..129C":298966.85928,"Abundance..130N":270784.7637,"Abundance..130C":261368.80811,"Abundance..131":263840.71372,"Ions.Score":45,"Identity.Strict":30,"Identity.Relaxed":23,"Expectation.Value":0.00035,"Percolator.q.Value":0,"Percolator.PEP":9.517e-08}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (B2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].iMDPNIVGSEHYDVAr.[G]","Modifications":"N-Term(TMT6plex); R16(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":685.68658,"MH...Da.":2055.0452,"Theo..MH...Da.":2055.04061,"DeltaM..ppm.":2.23,"Deltam.z..Da.":0.00153,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":13.98502,"Average.Reporter.S.N":23.2,"Ion.Inject.Time..ms.":6.761,"RT..min.":131.0538,"First.Scan":67543,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_01.raw","File.ID":"F14","Abundance..126":58761.38117,"Abundance..127N":84543.65973,"Abundance..127C":58260.16085,"Abundance..128N":87394.75693,"Abundance..128C":68085.17874,"Abundance..129N":80504.17295,"Abundance..129C":62381.71385,"Abundance..130N":74926.67718,"Abundance..130C":88210.66534,"Abundance..131":70673.51679,"Ions.Score":48,"Identity.Strict":26,"Identity.Relaxed":19,"Expectation.Value":0.00006,"Percolator.q.Value":0,"Percolator.PEP":5.332e-07}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (B2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].fcTGLTQIETLFk.[S]","Modifications":"N-Term(TMT6plex); C2(Carbamidomethyl); K13(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P12277","Master.Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","Protein.Accessions":"P12277","Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":0.9718,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":1012.57373,"MH...Da.":2024.14018,"Theo..MH...Da.":2024.1382,"DeltaM..ppm.":0.98,"Deltam.z..Da.":0.00099,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":0,"Average.Reporter.S.N":29.4,"Ion.Inject.Time..ms.":11.265,"RT..min.":196.2057,"First.Scan":104988,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_01.raw","File.ID":"F14","Abundance..126":25328.19516,"Abundance..127N":20518.89009,"Abundance..127C":26779.68092,"Abundance..128N":27528.64009,"Abundance..128C":28018.3768,"Abundance..129N":27952.5355,"Abundance..129C":22196.6219,"Abundance..130N":26789.10441,"Abundance..130C":24542.95511,"Abundance..131":25746.93517,"Ions.Score":71,"Identity.Strict":30,"Identity.Relaxed":23,"Expectation.Value":8.48066e-07,"Percolator.q.Value":0,"Percolator.PEP":5.479e-08}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (B2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].lISWYDNEFGYSNr.[V]","Modifications":"N-Term(TMT6plex); R14(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":668.33008,"MH...Da.":2002.97568,"Theo..MH...Da.":2002.97358,"DeltaM..ppm.":1.05,"Deltam.z..Da.":0.0007,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":14.82982,"Average.Reporter.S.N":40.6,"Ion.Inject.Time..ms.":2.174,"RT..min.":177.9091,"First.Scan":94710,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_01.raw","File.ID":"F14","Abundance..126":550733.67896,"Abundance..127N":541841.21908,"Abundance..127C":611086.29719,"Abundance..128N":499544.59004,"Abundance..128C":520664.20715,"Abundance..129N":571364.88749,"Abundance..129C":562506.0557,"Abundance..130N":518976.56494,"Abundance..130C":455441.45678,"Abundance..131":491855.60005,"Ions.Score":45,"Identity.Strict":24,"Identity.Relaxed":17,"Expectation.Value":0.00009,"Percolator.q.Value":0,"Percolator.PEP":1.091e-06}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (B2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].vIHDNFGIVEGLMTTVHAITATQk.[T]","Modifications":"N-Term(TMT6plex); K24(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":0,"Charge":4,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":766.18195,"MH...Da.":3061.70595,"Theo..MH...Da.":3061.7,"DeltaM..ppm.":1.95,"Deltam.z..Da.":0.00149,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":11.17531,"Average.Reporter.S.N":9.4,"Ion.Inject.Time..ms.":14.929,"RT..min.":194.7828,"First.Scan":104204,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_01.raw","File.ID":"F14","Abundance..126":6859.38121,"Abundance..127N":5266.61405,"Abundance..127C":5373.22138,"Abundance..128N":26538.23853,"Abundance..128C":8237.00677,"Abundance..129N":8634.05268,"Abundance..129C":9769.04872,"Abundance..130N":6753.69632,"Abundance..130C":12739.16469,"Abundance..131":18905.75257,"Ions.Score":27,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.01738,"Percolator.q.Value":0,"Percolator.PEP":8.411e-07}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (B2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].dYEFMWNPHLGYILTcPSNLGTGLr.[A]","Modifications":"N-Term(TMT6plex); C16(Carbamidomethyl); R25(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P12277","Master.Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","Protein.Accessions":"P12277","Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","X..Missed.Cleavages":0,"Charge":4,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":799.14789,"MH...Da.":3193.56972,"Theo..MH...Da.":3193.5676,"DeltaM..ppm.":0.67,"Deltam.z..Da.":0.00053,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":15.77866,"Average.Reporter.S.N":37.5,"Ion.Inject.Time..ms.":16.246,"RT..min.":190.5326,"First.Scan":101837,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_01.raw","File.ID":"F14","Abundance..126":46371.36952,"Abundance..127N":48867.01969,"Abundance..127C":54603.23974,"Abundance..128N":52339.66693,"Abundance..128C":60971.11111,"Abundance..129N":56362.62942,"Abundance..129C":56373.16647,"Abundance..130N":54826.59568,"Abundance..130C":59267.74945,"Abundance..131":50565.72034,"Ions.Score":13,"Identity.Strict":26,"Identity.Relaxed":19,"Expectation.Value":0.22827,"Percolator.q.Value":0,"Percolator.PEP":0.00002}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (B2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].hDMAAAGFTEALTFALcSQEDIADk.[L]","Modifications":"N-Term(TMT6plex); C17(Carbamidomethyl); K25(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q9NSD9","Master.Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","Protein.Accessions":"Q9NSD9","Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":1060.19409,"MH...Da.":3178.56772,"Theo..MH...Da.":3178.56806,"DeltaM..ppm.":-0.1,"Deltam.z..Da.":-0.00011,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":26.2484,"Average.Reporter.S.N":10.5,"Ion.Inject.Time..ms.":21.456,"RT..min.":199.3417,"First.Scan":106689,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_01.raw","File.ID":"F14","Abundance..126":2979.97602,"Abundance..127N":4298.593,"Abundance..127C":4235.77852,"Abundance..128N":5179.93718,"Abundance..128C":5003.34946,"Abundance..129N":4878.73848,"Abundance..129C":4038.75813,"Abundance..130N":3258.56629,"Abundance..130C":4513.32181,"Abundance..131":4953.20768,"Ions.Score":50,"Identity.Strict":25,"Identity.Relaxed":18,"Expectation.Value":0.00004,"Percolator.q.Value":0,"Percolator.PEP":5.585e-07}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (N2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[R].fAQPGSFEYEYAMr.[W]","Modifications":"N-Term(TMT6plex); R14(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q15233","Master.Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","Protein.Accessions":"Q15233","Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":967.9635,"MH...Da.":1934.91973,"Theo..MH...Da.":1934.91837,"DeltaM..ppm.":0.7,"Deltam.z..Da.":0.00068,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":29.99925,"Average.Reporter.S.N":37.1,"Ion.Inject.Time..ms.":46.163,"RT..min.":154.0863,"First.Scan":78172,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_03.raw","File.ID":"F2","Abundance..126":17699.46643,"Abundance..127N":16976.81927,"Abundance..127C":17353.89919,"Abundance..128N":14253.16472,"Abundance..128C":19795.66721,"Abundance..129N":17509.67831,"Abundance..129C":17925.54444,"Abundance..130N":18608.70203,"Abundance..130C":18141.56597,"Abundance..131":18650.50927,"Ions.Score":61,"Identity.Strict":22,"Identity.Relaxed":15,"Expectation.Value":1.24486e-06,"Percolator.q.Value":0,"Percolator.PEP":0.00005}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (N2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].lISWYDNEFGYSNr.[V]","Modifications":"N-Term(TMT6plex); R14(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":668.33026,"MH...Da.":2002.97623,"Theo..MH...Da.":2002.97358,"DeltaM..ppm.":1.33,"Deltam.z..Da.":0.00088,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":6.59776,"Average.Reporter.S.N":50,"Ion.Inject.Time..ms.":3.615,"RT..min.":176.9883,"First.Scan":91334,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_03.raw","File.ID":"F2","Abundance..126":606862.09654,"Abundance..127N":564039.6001,"Abundance..127C":689210.92808,"Abundance..128N":585585.27864,"Abundance..128C":662652.28557,"Abundance..129N":613425.80212,"Abundance..129C":689992.80884,"Abundance..130N":670147.8379,"Abundance..130C":599363.32777,"Abundance..131":612833.17081,"Ions.Score":36,"Identity.Strict":24,"Identity.Relaxed":17,"Expectation.Value":0.00082,"Percolator.q.Value":0,"Percolator.PEP":0.00007}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (N2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].lISWYDNEFGYSNr.[V]","Modifications":"N-Term(TMT6plex); R14(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":1001.99139,"MH...Da.":2002.97551,"Theo..MH...Da.":2002.97358,"DeltaM..ppm.":0.97,"Deltam.z..Da.":0.00097,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":13.20243,"Average.Reporter.S.N":31.9,"Ion.Inject.Time..ms.":14.688,"RT..min.":176.9408,"First.Scan":91307,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_03.raw","File.ID":"F2","Abundance..126":21964.68807,"Abundance..127N":21381.59779,"Abundance..127C":22753.94619,"Abundance..128N":23063.00232,"Abundance..128C":19361.56228,"Abundance..129N":18754.78724,"Abundance..129C":25816.56594,"Abundance..130N":22233.61385,"Abundance..130C":22749.88415,"Abundance..131":22040.02258,"Ions.Score":83,"Identity.Strict":24,"Identity.Relaxed":17,"Expectation.Value":1.68314e-08,"Percolator.q.Value":0,"Percolator.PEP":1.017e-06}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (B2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].lVINGNPITIFQErDPSk.[I]","Modifications":"N-Term(TMT6plex); R14(Label:13C(6)15N(4)); K18(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":1,"Charge":3,"DeltaScore":0.8485,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":839.82446,"MH...Da.":2517.45884,"Theo..MH...Da.":2517.456,"DeltaM..ppm.":1.13,"Deltam.z..Da.":0.00095,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":11.91535,"Average.Reporter.S.N":47.7,"Ion.Inject.Time..ms.":5.397,"RT..min.":166.2991,"First.Scan":88034,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_01.raw","File.ID":"F14","Abundance..126":156664.59556,"Abundance..127N":175958.7249,"Abundance..127C":162699.94513,"Abundance..128N":166448.26511,"Abundance..128C":167872.70061,"Abundance..129N":172975.60515,"Abundance..129C":162084.2682,"Abundance..130N":187909.92237,"Abundance..130C":176547.04697,"Abundance..131":170851.16823,"Ions.Score":33,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.00481,"Percolator.q.Value":0,"Percolator.PEP":3.947e-07}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (B2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].rVIISAPSADAPMFVmGVNHEk.[Y]","Modifications":"N-Term(TMT6plex); R1(Label:13C(6)15N(4)); M16(Oxidation); K22(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":1,"Charge":4,"DeltaScore":0.3571,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":716.14532,"MH...Da.":2861.55947,"Theo..MH...Da.":2861.55368,"DeltaM..ppm.":2.02,"Deltam.z..Da.":0.00145,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":15.52026,"Average.Reporter.S.N":18.3,"Ion.Inject.Time..ms.":17.222,"RT..min.":139.3912,"First.Scan":72311,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_01.raw","File.ID":"F14","Abundance..126":32040.92482,"Abundance..127N":32514.52829,"Abundance..127C":32231.01158,"Abundance..128N":37176.79146,"Abundance..128C":42875.99042,"Abundance..129N":31598.2628,"Abundance..129C":32184.28014,"Abundance..130N":39888.72878,"Abundance..130C":39274.09723,"Abundance..131":38339.89282,"Ions.Score":42,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.00053,"Percolator.q.Value":0,"Percolator.PEP":3.86e-08}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (B2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[K].rVIISAPSADAPMFVmGVNHEk.[Y]","Modifications":"N-Term(TMT6plex); R1(Label:13C(6)15N(4)); M16(Oxidation); K22(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":1,"Charge":4,"DeltaScore":0.2857,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":716.14441,"MH...Da.":2861.55581,"Theo..MH...Da.":2861.55368,"DeltaM..ppm.":0.74,"Deltam.z..Da.":0.00053,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":65.85563,"Average.Reporter.S.N":20.8,"Ion.Inject.Time..ms.":12.392,"RT..min.":137.0269,"First.Scan":70971,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_01.raw","File.ID":"F14","Abundance..126":28180.58338,"Abundance..127N":28513.3252,"Abundance..127C":20825.94056,"Abundance..128N":19073.67982,"Abundance..128C":30376.41355,"Abundance..129N":22595.56454,"Abundance..129C":28877.45543,"Abundance..130N":28483.92358,"Abundance..130C":26878.39009,"Abundance..131":23222.73712,"Ions.Score":28,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.01404,"Percolator.q.Value":0,"Percolator.PEP":6.28e-07}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (B2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].rVIISAPSADAPmFVMGVNHEk.[Y]","Modifications":"N-Term(TMT6plex); R1(Label:13C(6)15N(4)); M13(Oxidation); K22(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":1,"Charge":4,"DeltaScore":0.5263,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":716.14471,"MH...Da.":2861.55703,"Theo..MH...Da.":2861.55368,"DeltaM..ppm.":1.17,"Deltam.z..Da.":0.00084,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":13.79598,"Average.Reporter.S.N":17.9,"Ion.Inject.Time..ms.":1.476,"RT..min.":133.5246,"First.Scan":68944,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_01.raw","File.ID":"F14","Abundance..126":96032.51062,"Abundance..127N":105374.96091,"Abundance..127C":112319.33003,"Abundance..128N":130685.23775,"Abundance..128C":108308.16874,"Abundance..129N":101996.8891,"Abundance..129C":96416.94095,"Abundance..130N":107051.08312,"Abundance..130C":114866.90406,"Abundance..131":104277.85961,"Ions.Score":38,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.00139,"Percolator.q.Value":0,"Percolator.PEP":8.822e-06}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (B2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].rVIISAPSADAPMFVMGVNHEk.[Y]","Modifications":"N-Term(TMT6plex); R1(Label:13C(6)15N(4)); K22(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":1,"Charge":4,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":712.14539,"MH...Da.":2845.55971,"Theo..MH...Da.":2845.55876,"DeltaM..ppm.":0.33,"Deltam.z..Da.":0.00024,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":5.8571,"Average.Reporter.S.N":89.6,"Ion.Inject.Time..ms.":1.721,"RT..min.":145.1182,"First.Scan":75663,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_01.raw","File.ID":"F14","Abundance..126":1304511.1739,"Abundance..127N":1315433.20503,"Abundance..127C":1547039.17765,"Abundance..128N":1409545.74115,"Abundance..128C":1425035.10722,"Abundance..129N":1277286.80285,"Abundance..129C":1419003.06359,"Abundance..130N":1376302.1227,"Abundance..130C":1482127.6763,"Abundance..131":1379187.73529,"Ions.Score":37,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.00177,"Percolator.q.Value":0,"Percolator.PEP":6.275e-09}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (B2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].qYPWGVAEVENGEHcDFTILr.[N]","Modifications":"N-Term(TMT6plex); C15(Carbamidomethyl); R21(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q16181","Master.Protein.Descriptions":"Septin-7 OS=Homo sapiens GN=SEPT7 PE=1 SV=2","Protein.Accessions":"Q16181","Protein.Descriptions":"Septin-7 OS=Homo sapiens GN=SEPT7 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":920.4505,"MH...Da.":2759.33695,"Theo..MH...Da.":2759.33243,"DeltaM..ppm.":1.64,"Deltam.z..Da.":0.0015,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":10.06087,"Average.Reporter.S.N":14.7,"Ion.Inject.Time..ms.":12.25,"RT..min.":165.4129,"First.Scan":87518,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_01.raw","File.ID":"F14","Abundance..126":26600.34022,"Abundance..127N":30577.73296,"Abundance..127C":30424.63998,"Abundance..128N":20131.32565,"Abundance..128C":24098.89463,"Abundance..129N":33806.97366,"Abundance..129C":26538.95156,"Abundance..130N":31202.39543,"Abundance..130C":22065.83213,"Abundance..131":28509.5242,"Ions.Score":74,"Identity.Strict":24,"Identity.Relaxed":17,"Expectation.Value":9.80241e-08,"Percolator.q.Value":0,"Percolator.PEP":2.742e-11}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (A2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[K].aAGASDVVLYk.[I]","Modifications":"N-Term(TMT6plex); K11(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q9NSD9","Master.Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","Protein.Accessions":"Q9NSD9","Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":0.8667,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":780.46924,"MH...Da.":1559.9312,"Theo..MH...Da.":1559.92886,"DeltaM..ppm.":1.5,"Deltam.z..Da.":0.00117,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":17.59008,"Average.Reporter.S.N":36.8,"Ion.Inject.Time..ms.":9.544,"RT..min.":122.5274,"First.Scan":59852,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_01.raw","File.ID":"F15","Abundance..126":52457.59266,"Abundance..127N":47683.58188,"Abundance..127C":50921.67017,"Abundance..128N":50033.79141,"Abundance..128C":56354.65138,"Abundance..129N":44098.01211,"Abundance..129C":57140.6245,"Abundance..130N":58118.1371,"Abundance..130C":48460.15112,"Abundance..131":59493.20103,"Ions.Score":60,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":9.3744e-06,"Percolator.q.Value":0,"Percolator.PEP":2.054e-06}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (O2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].nLEGYVGFANLPNQVYr.[K]","Modifications":"N-Term(TMT6plex); R17(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q16181","Master.Protein.Descriptions":"Septin-7 OS=Homo sapiens GN=SEPT7 PE=1 SV=2","Protein.Accessions":"Q16181","Protein.Descriptions":"Septin-7 OS=Homo sapiens GN=SEPT7 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":1097.08252,"MH...Da.":2193.15776,"Theo..MH...Da.":2193.15294,"DeltaM..ppm.":2.2,"Deltam.z..Da.":0.00241,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":0,"Average.Reporter.S.N":7.5,"Ion.Inject.Time..ms.":50,"RT..min.":169.506,"First.Scan":88880,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_03.raw","File.ID":"F1","Abundance..126":3998.28407,"Abundance..127N":4039.49696,"Abundance..127C":3974.40239,"Abundance..128N":2590.56688,"Abundance..128C":4311.98173,"Abundance..129N":3518.93458,"Abundance..129C":3496.15873,"Abundance..130N":4589.47874,"Abundance..130C":3915.85207,"Abundance..131":4758.76072,"Ions.Score":75,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":2.70185e-07,"Percolator.q.Value":0,"Percolator.PEP":2.234e-07}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (A2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[R].hLcAVYYNk.[N]","Modifications":"N-Term(TMT6plex); C3(Carbamidomethyl); K9(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q9NSD9","Master.Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","Protein.Accessions":"Q9NSD9","Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":545.30536,"MH...Da.":1633.90152,"Theo..MH...Da.":1633.9016,"DeltaM..ppm.":-0.05,"Deltam.z..Da.":-0.00003,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":16.02362,"Average.Reporter.S.N":56.1,"Ion.Inject.Time..ms.":7.471,"RT..min.":86.7317,"First.Scan":39454,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_01.raw","File.ID":"F15","Abundance..126":184826.55104,"Abundance..127N":155477.41372,"Abundance..127C":175791.47096,"Abundance..128N":142521.63923,"Abundance..128C":193568.48563,"Abundance..129N":163870.87802,"Abundance..129C":164777.05946,"Abundance..130N":171908.22843,"Abundance..130C":176570.51824,"Abundance..131":163332.19505,"Ions.Score":27,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":0.01677,"Percolator.q.Value":0,"Percolator.PEP":0.00002}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (O2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].iMDPNIVGSEHYDVAr.[G]","Modifications":"N-Term(TMT6plex); R16(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":685.68597,"MH...Da.":2055.04337,"Theo..MH...Da.":2055.04061,"DeltaM..ppm.":1.34,"Deltam.z..Da.":0.00092,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":11.3585,"Average.Reporter.S.N":25.7,"Ion.Inject.Time..ms.":3.416,"RT..min.":130.0596,"First.Scan":65948,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_03.raw","File.ID":"F1","Abundance..126":45150.28072,"Abundance..127N":86824.46469,"Abundance..127C":97208.38091,"Abundance..128N":138688.25842,"Abundance..128C":106129.16982,"Abundance..129N":105721.17144,"Abundance..129C":116223.94755,"Abundance..130N":100535.59922,"Abundance..130C":93087.32475,"Abundance..131":120710.20206,"Ions.Score":47,"Identity.Strict":26,"Identity.Relaxed":19,"Expectation.Value":0.00009,"Percolator.q.Value":0,"Percolator.PEP":9.55e-06}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (O2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[R].lFVGNLPPDITEEEMr.[K]","Modifications":"N-Term(TMT6plex); R16(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q15233","Master.Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","Protein.Accessions":"Q15233","Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":0.863,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":1050.05054,"MH...Da.":2099.0938,"Theo..MH...Da.":2099.09198,"DeltaM..ppm.":0.87,"Deltam.z..Da.":0.00091,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":0,"Average.Reporter.S.N":27.8,"Ion.Inject.Time..ms.":18.311,"RT..min.":177.1567,"First.Scan":93306,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_03.raw","File.ID":"F1","Abundance..126":10441.6466,"Abundance..127N":10160.75441,"Abundance..127C":10610.54392,"Abundance..128N":12372.80383,"Abundance..128C":10979.27464,"Abundance..129N":9287.27743,"Abundance..129C":11476.16886,"Abundance..130N":11074.90622,"Abundance..130C":10076.4133,"Abundance..131":11744.65844,"Ions.Score":73,"Identity.Strict":27,"Identity.Relaxed":20,"Expectation.Value":2.79097e-07,"Percolator.q.Value":0,"Percolator.PEP":2.029e-07}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (A2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].tIAMDGTEGLVr.[G]","Modifications":"N-Term(TMT6plex); R12(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":0.8387,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":751.4101,"MH...Da.":1501.81291,"Theo..MH...Da.":1501.81211,"DeltaM..ppm.":0.53,"Deltam.z..Da.":0.0004,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":17.48868,"Average.Reporter.S.N":34.6,"Ion.Inject.Time..ms.":4.182,"RT..min.":127.0977,"First.Scan":62446,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_01.raw","File.ID":"F15","Abundance..126":195763.93359,"Abundance..127N":193004.96617,"Abundance..127C":172271.18596,"Abundance..128N":146159.69493,"Abundance..128C":181015.56003,"Abundance..129N":177043.58037,"Abundance..129C":177712.48145,"Abundance..130N":176831.27704,"Abundance..130C":212670.64996,"Abundance..131":179486.15763,"Ions.Score":62,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":4.64684e-06,"Percolator.q.Value":0,"Percolator.PEP":2.843e-06}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (O2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].iISNAScTTNcLAPLAk.[V]","Modifications":"N-Term(TMT6plex); C7(Carbamidomethyl); C11(Carbamidomethyl); K17(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.72,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":767.42749,"MH...Da.":2300.26792,"Theo..MH...Da.":2300.25979,"DeltaM..ppm.":3.53,"Deltam.z..Da.":0.00271,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":32.24433,"Average.Reporter.S.N":115.5,"Ion.Inject.Time..ms.":5.426,"RT..min.":149.1639,"First.Scan":77049,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_03.raw","File.ID":"F1","Abundance..126":351284.55352,"Abundance..127N":317806.80885,"Abundance..127C":391192.96186,"Abundance..128N":387437.14413,"Abundance..128C":355760.75452,"Abundance..129N":301651.11073,"Abundance..129C":391124.70165,"Abundance..130N":360345.22644,"Abundance..130C":336041.99459,"Abundance..131":338812.55616,"Ions.Score":50,"Identity.Strict":30,"Identity.Relaxed":23,"Expectation.Value":0.0001,"Percolator.q.Value":0,"Percolator.PEP":2.031e-07}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (O2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].aDTLTPEEcQQFkk.[Q]","Modifications":"N-Term(TMT6plex); C9(Carbamidomethyl); K13(Label); K14(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q16181","Master.Protein.Descriptions":"Septin-7 OS=Homo sapiens GN=SEPT7 PE=1 SV=2","Protein.Accessions":"Q16181","Protein.Descriptions":"Septin-7 OS=Homo sapiens GN=SEPT7 PE=1 SV=2","X..Missed.Cleavages":1,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":800.11243,"MH...Da.":2398.32273,"Theo..MH...Da.":2398.32261,"DeltaM..ppm.":0.05,"Deltam.z..Da.":0.00004,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":20.58542,"Average.Reporter.S.N":19.5,"Ion.Inject.Time..ms.":10.411,"RT..min.":109.9948,"First.Scan":54157,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_03.raw","File.ID":"F1","Abundance..126":18833.99928,"Abundance..127N":18919.75762,"Abundance..127C":18156.89845,"Abundance..128N":29457.05275,"Abundance..128C":26361.14042,"Abundance..129N":21510.65928,"Abundance..129C":26162.25354,"Abundance..130N":18809.07423,"Abundance..130C":27435.99775,"Abundance..131":25408.5015,"Ions.Score":15,"Identity.Strict":30,"Identity.Relaxed":23,"Expectation.Value":0.30997,"Percolator.q.Value":0,"Percolator.PEP":0.00002}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (O2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].kMEMEMEQVFEMk.[V]","Modifications":"N-Term(TMT6plex); K1(Label); K13(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q16181","Master.Protein.Descriptions":"Septin-7 OS=Homo sapiens GN=SEPT7 PE=1 SV=2","Protein.Accessions":"Q16181","Protein.Descriptions":"Septin-7 OS=Homo sapiens GN=SEPT7 PE=1 SV=2","X..Missed.Cleavages":1,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":798.42236,"MH...Da.":2393.25254,"Theo..MH...Da.":2393.25268,"DeltaM..ppm.":-0.06,"Deltam.z..Da.":-0.00005,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":30.1587,"Average.Reporter.S.N":22.3,"Ion.Inject.Time..ms.":6.586,"RT..min.":166.2773,"First.Scan":87011,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_03.raw","File.ID":"F1","Abundance..126":53500.38218,"Abundance..127N":57448.83852,"Abundance..127C":54860.48181,"Abundance..128N":46255.87554,"Abundance..128C":63771.35483,"Abundance..129N":65767.89167,"Abundance..129C":63348.74099,"Abundance..130N":52570.22182,"Abundance..130C":52474.08115,"Abundance..131":65499.69764,"Ions.Score":29,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":0.01056,"Percolator.q.Value":0,"Percolator.PEP":0.00005}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (O2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].fGQAATMEGIGAIGGTPPAFNr.[A]","Modifications":"N-Term(TMT6plex); R22(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q15233","Master.Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","Protein.Accessions":"Q15233","Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":801.41797,"MH...Da.":2402.23935,"Theo..MH...Da.":2402.23635,"DeltaM..ppm.":1.25,"Deltam.z..Da.":0.001,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":11.83126,"Average.Reporter.S.N":17.2,"Ion.Inject.Time..ms.":5.439,"RT..min.":168.7876,"First.Scan":88483,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_03.raw","File.ID":"F1","Abundance..126":26306.23474,"Abundance..127N":21964.06867,"Abundance..127C":36692.69713,"Abundance..128N":26947.75538,"Abundance..128C":30682.08592,"Abundance..129N":29490.30442,"Abundance..129C":29133.92402,"Abundance..130N":25827.83631,"Abundance..130C":31329.55267,"Abundance..131":31044.93966,"Ions.Score":47,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":0.00015,"Percolator.q.Value":0,"Percolator.PEP":8.53e-06}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (O2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[R].vPTANVSVVDLTcrLEk.[P]","Modifications":"N-Term(TMT6plex); C13(Carbamidomethyl); R14(Label:13C(6)15N(4)); K17(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":1,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":793.12726,"MH...Da.":2377.36722,"Theo..MH...Da.":2377.36441,"DeltaM..ppm.":1.18,"Deltam.z..Da.":0.00094,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":1.65243,"Average.Reporter.S.N":34.1,"Ion.Inject.Time..ms.":2.085,"RT..min.":154.5071,"First.Scan":80147,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_03.raw","File.ID":"F1","Abundance..126":405851.20143,"Abundance..127N":361925.59955,"Abundance..127C":348948.13136,"Abundance..128N":374930.39417,"Abundance..128C":451026.61099,"Abundance..129N":389061.05844,"Abundance..129C":354172.01492,"Abundance..130N":358598.89851,"Abundance..130C":373336.74039,"Abundance..131":428768.20355,"Ions.Score":43,"Identity.Strict":30,"Identity.Relaxed":23,"Expectation.Value":0.00063,"Percolator.q.Value":0,"Percolator.PEP":1.377e-06}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (O2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].rGTGGVDTAAVGGVFDVSNADr.[L]","Modifications":"N-Term(TMT6plex); R1(Label:13C(6)15N(4)); R22(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P12277","Master.Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","Protein.Accessions":"P12277","Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":790.74188,"MH...Da.":2370.21109,"Theo..MH...Da.":2370.2114,"DeltaM..ppm.":-0.13,"Deltam.z..Da.":-0.0001,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":0,"Average.Reporter.S.N":26.5,"Ion.Inject.Time..ms.":5.69,"RT..min.":120.5562,"First.Scan":60396,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_03.raw","File.ID":"F1","Abundance..126":101763.55679,"Abundance..127N":104560.23969,"Abundance..127C":122387.13145,"Abundance..128N":99946.13924,"Abundance..128C":105536.35577,"Abundance..129N":105725.98771,"Abundance..129C":110974.59211,"Abundance..130N":129519.66937,"Abundance..130C":100035.84021,"Abundance..131":122531.79365,"Ions.Score":77,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":1.32203e-07,"Percolator.q.Value":0,"Percolator.PEP":2.585e-08}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (O2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].iYEFPETDDEEENk.[L]","Modifications":"N-Term(TMT6plex); K14(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q16181","Master.Protein.Descriptions":"Septin-7 OS=Homo sapiens GN=SEPT7 PE=1 SV=2","Protein.Accessions":"Q16181","Protein.Descriptions":"Septin-7 OS=Homo sapiens GN=SEPT7 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":742.03265,"MH...Da.":2224.08341,"Theo..MH...Da.":2224.07889,"DeltaM..ppm.":2.03,"Deltam.z..Da.":0.0015,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":37.78769,"Average.Reporter.S.N":30.2,"Ion.Inject.Time..ms.":2.354,"RT..min.":147.1146,"First.Scan":75854,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_03.raw","File.ID":"F1","Abundance..126":165419.30351,"Abundance..127N":159754.46129,"Abundance..127C":144408.66147,"Abundance..128N":198012.44339,"Abundance..128C":180791.90844,"Abundance..129N":146352.71846,"Abundance..129C":184625.86549,"Abundance..130N":162743.98254,"Abundance..130C":166900.63305,"Abundance..131":176552.29616,"Ions.Score":32,"Identity.Strict":24,"Identity.Relaxed":17,"Expectation.Value":0.00194,"Percolator.q.Value":0,"Percolator.PEP":0.00001}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (O2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].aIAELGIYPAVDPLDSTSr.[I]","Modifications":"N-Term(TMT6plex); R19(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":1114.10657,"MH...Da.":2227.20586,"Theo..MH...Da.":2227.2047,"DeltaM..ppm.":0.52,"Deltam.z..Da.":0.00058,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":0,"Average.Reporter.S.N":35.1,"Ion.Inject.Time..ms.":31.611,"RT..min.":184.852,"First.Scan":97763,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_03.raw","File.ID":"F1","Abundance..126":73945.99393,"Abundance..127N":81302.35038,"Abundance..127C":78741.57665,"Abundance..128N":81077.61805,"Abundance..128C":77596.172,"Abundance..129N":72367.94196,"Abundance..129C":79228.82352,"Abundance..130N":73024.4742,"Abundance..130C":85542.31744,"Abundance..131":73708.75686,"Ions.Score":59,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.00001,"Percolator.q.Value":0,"Percolator.PEP":3.692e-07}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (A2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].rGALIVLEGVDr.[A]","Modifications":"N-Term(TMT6plex); R1(Label:13C(6)15N(4)); R12(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P23919","Master.Protein.Descriptions":"Thymidylate kinase OS=Homo sapiens GN=DTYMK PE=1 SV=4","Protein.Accessions":"P23919","Protein.Descriptions":"Thymidylate kinase OS=Homo sapiens GN=DTYMK PE=1 SV=4","X..Missed.Cleavages":1,"Charge":3,"DeltaScore":0.8438,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":516.31787,"MH...Da.":1546.93906,"Theo..MH...Da.":1546.93813,"DeltaM..ppm.":0.6,"Deltam.z..Da.":0.00031,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":29.54682,"Average.Reporter.S.N":54.8,"Ion.Inject.Time..ms.":6.414,"RT..min.":117.8119,"First.Scan":57150,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_01.raw","File.ID":"F15","Abundance..126":147487.53923,"Abundance..127N":116690.57225,"Abundance..127C":111424.08718,"Abundance..128N":137440.80212,"Abundance..128C":134209.29089,"Abundance..129N":128505.56461,"Abundance..129C":135107.89615,"Abundance..130N":135167.45989,"Abundance..130C":125199.52653,"Abundance..131":119451.34267,"Ions.Score":32,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.00647,"Percolator.q.Value":0,"Percolator.PEP":0.00004}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (A2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[R].vPTANVSVVDLTcr.[L]","Modifications":"N-Term(TMT6plex); C13(Carbamidomethyl); R14(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":0.9839,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":885.48737,"MH...Da.":1769.96745,"Theo..MH...Da.":1769.96565,"DeltaM..ppm.":1.02,"Deltam.z..Da.":0.0009,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":20.83183,"Average.Reporter.S.N":6.5,"Ion.Inject.Time..ms.":2.933,"RT..min.":147.8461,"First.Scan":74350,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_01.raw","File.ID":"F15","Abundance..126":91433.16759,"Abundance..127N":76663.26439,"Abundance..127C":82118.9731,"Abundance..128N":54130.87105,"Abundance..128C":69764.29285,"Abundance..129N":68252.99604,"Abundance..129C":50328.26167,"Abundance..130N":78902.26306,"Abundance..130C":41476.76629,"Abundance..131":93382.68321,"Ions.Score":62,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":5.48234e-06,"Percolator.q.Value":0,"Percolator.PEP":2.689e-06}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (O2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].tFLVWVNEEDHLr.[V]","Modifications":"N-Term(TMT6plex); R13(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P12277","Master.Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","Protein.Accessions":"P12277","Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":633.00659,"MH...Da.":1897.00522,"Theo..MH...Da.":1897.00448,"DeltaM..ppm.":0.39,"Deltam.z..Da.":0.00025,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":6.24429,"Average.Reporter.S.N":27.7,"Ion.Inject.Time..ms.":0.821,"RT..min.":160.1277,"First.Scan":83419,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_03.raw","File.ID":"F1","Abundance..126":275053.77112,"Abundance..127N":230300.07745,"Abundance..127C":285007.89284,"Abundance..128N":259158.59456,"Abundance..128C":328687.37424,"Abundance..129N":293436.62938,"Abundance..129C":233875.64921,"Abundance..130N":263451.43676,"Abundance..130C":284325.52423,"Abundance..131":335811.3959,"Ions.Score":58,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.00001,"Percolator.q.Value":0,"Percolator.PEP":1.07e-06}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (O2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].sTLINSLFLTDLYSPEYPGPSHr.[I]","Modifications":"N-Term(TMT6plex); R23(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q16181","Master.Protein.Descriptions":"Septin-7 OS=Homo sapiens GN=SEPT7 PE=1 SV=2","Protein.Accessions":"Q16181","Protein.Descriptions":"Septin-7 OS=Homo sapiens GN=SEPT7 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":949.49792,"MH...Da.":2846.47922,"Theo..MH...Da.":2846.48014,"DeltaM..ppm.":-0.32,"Deltam.z..Da.":-0.00031,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":10.81828,"Average.Reporter.S.N":3.6,"Ion.Inject.Time..ms.":8.183,"RT..min.":188.8763,"First.Scan":100045,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_03.raw","File.ID":"F1","Abundance..126":4564.82467,"Abundance..127N":5383.01568,"Abundance..127C":4313.69497,"Abundance..128N":3296.23063,"Abundance..128C":4562.85711,"Abundance..129N":4693.28398,"Abundance..129C":4901.53093,"Abundance..130N":4669.59565,"Abundance..131":5369.10188,"Ions.Score":35,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.00258,"Percolator.q.Value":0,"Percolator.PEP":1.271e-06}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (O2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].rVIISAPSADAPmFVMGVNHEk.[Y]","Modifications":"N-Term(TMT6plex); R1(Label:13C(6)15N(4)); M13(Oxidation); K22(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":1,"Charge":4,"DeltaScore":0.3182,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":716.14319,"MH...Da.":2861.55092,"Theo..MH...Da.":2861.55368,"DeltaM..ppm.":-0.96,"Deltam.z..Da.":-0.00069,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":33.60228,"Average.Reporter.S.N":11.2,"Ion.Inject.Time..ms.":4.929,"RT..min.":142.2815,"First.Scan":73023,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_03.raw","File.ID":"F1","Abundance..126":24853.27058,"Abundance..127N":36803.3793,"Abundance..127C":37264.14045,"Abundance..128N":37378.98083,"Abundance..128C":25456.11249,"Abundance..129N":28761.47967,"Abundance..129C":40876.26256,"Abundance..130N":37183.98034,"Abundance..130C":33396.68837,"Abundance..131":29762.14713,"Ions.Score":22,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.05355,"Percolator.q.Value":0,"Percolator.PEP":0.0002}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (O2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].wGDAGAEYVVESTGVFTTMEk.[A]","Modifications":"N-Term(TMT6plex); K21(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":915.13232,"MH...Da.":2743.38242,"Theo..MH...Da.":2743.37805,"DeltaM..ppm.":1.59,"Deltam.z..Da.":0.00146,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":5.04516,"Average.Reporter.S.N":16.2,"Ion.Inject.Time..ms.":1.013,"RT..min.":194.8612,"First.Scan":103388,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_03.raw","File.ID":"F1","Abundance..126":120268.57405,"Abundance..127N":115884.80594,"Abundance..127C":134225.40106,"Abundance..128N":147861.10574,"Abundance..128C":162546.98771,"Abundance..129N":131829.50024,"Abundance..129C":141401.0851,"Abundance..130N":170012.48281,"Abundance..130C":128324.95552,"Abundance..131":175577.63822,"Ions.Score":42,"Identity.Strict":27,"Identity.Relaxed":20,"Expectation.Value":0.00032,"Percolator.q.Value":0,"Percolator.PEP":0.00001}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (O2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].eQSIcAAEEQPAEDGQGETNk.[N]","Modifications":"N-Term(TMT6plex); C5(Carbamidomethyl); K21(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q9UGP8","Master.Protein.Descriptions":"Translocation protein SEC63 homolog OS=Homo sapiens GN=SEC63 PE=1 SV=2","Protein.Accessions":"Q9UGP8","Protein.Descriptions":"Translocation protein SEC63 homolog OS=Homo sapiens GN=SEC63 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":919.77679,"MH...Da.":2757.31583,"Theo..MH...Da.":2757.31289,"DeltaM..ppm.":1.07,"Deltam.z..Da.":0.00098,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":0,"Average.Reporter.S.N":9.6,"Ion.Inject.Time..ms.":50,"RT..min.":93.9695,"First.Scan":44844,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_03.raw","File.ID":"F1","Abundance..126":4985.31083,"Abundance..127N":5967.83744,"Abundance..127C":6561.49577,"Abundance..128N":8673.48755,"Abundance..128C":6478.6715,"Abundance..129N":4498.52075,"Abundance..129C":4885.37238,"Abundance..130N":6594.54844,"Abundance..130C":4983.50774,"Abundance..131":5599.44915,"Ions.Score":44,"Identity.Strict":24,"Identity.Relaxed":17,"Expectation.Value":0.00012,"Percolator.q.Value":0,"Percolator.PEP":3.55e-07}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (O2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].aLVAIGTHDLDTLSGPFTYTAk.[R]","Modifications":"N-Term(TMT6plex); K22(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q9NSD9","Master.Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","Protein.Accessions":"Q9NSD9","Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":919.84955,"MH...Da.":2757.53409,"Theo..MH...Da.":2757.53185,"DeltaM..ppm.":0.81,"Deltam.z..Da.":0.00075,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":11.11489,"Average.Reporter.S.N":8.2,"Ion.Inject.Time..ms.":3.611,"RT..min.":176.0555,"First.Scan":92675,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_03.raw","File.ID":"F1","Abundance..126":24343.31191,"Abundance..127N":27314.86387,"Abundance..127C":39122.05976,"Abundance..128N":30378.20365,"Abundance..128C":38348.96842,"Abundance..129N":37432.17061,"Abundance..129C":31540.8676,"Abundance..130N":19659.79486,"Abundance..130C":34056.37717,"Abundance..131":42943.6572,"Ions.Score":62,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":5.87867e-06,"Percolator.q.Value":0,"Percolator.PEP":1.201e-08}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (O2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].wGDAGAEYVVESTGVFTTmEk.[A]","Modifications":"N-Term(TMT6plex); M19(Oxidation); K21(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":920.46344,"MH...Da.":2759.37577,"Theo..MH...Da.":2759.37297,"DeltaM..ppm.":1.01,"Deltam.z..Da.":0.00093,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":49.44262,"Average.Reporter.S.N":15.2,"Ion.Inject.Time..ms.":15.866,"RT..min.":190.2624,"First.Scan":100824,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_03.raw","File.ID":"F1","Abundance..126":21457.03111,"Abundance..127N":21381.76505,"Abundance..127C":20842.34113,"Abundance..128N":24551.18843,"Abundance..128C":23319.14243,"Abundance..129N":19160.95221,"Abundance..129C":24861.45462,"Abundance..130N":24902.03646,"Abundance..130C":23389.94422,"Abundance..131":21812.69857,"Ions.Score":39,"Identity.Strict":26,"Identity.Relaxed":19,"Expectation.Value":0.00059,"Percolator.q.Value":0,"Percolator.PEP":4.916e-07}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (O2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].qYPWGVAEVENGEHcDFTILr.[N]","Modifications":"N-Term(TMT6plex); C15(Carbamidomethyl); R21(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q16181","Master.Protein.Descriptions":"Septin-7 OS=Homo sapiens GN=SEPT7 PE=1 SV=2","Protein.Accessions":"Q16181","Protein.Descriptions":"Septin-7 OS=Homo sapiens GN=SEPT7 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":920.45178,"MH...Da.":2759.34079,"Theo..MH...Da.":2759.33243,"DeltaM..ppm.":3.03,"Deltam.z..Da.":0.00279,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":13.37343,"Average.Reporter.S.N":11.1,"Ion.Inject.Time..ms.":10.524,"RT..min.":164.5781,"First.Scan":86026,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_03.raw","File.ID":"F1","Abundance..126":10335.67789,"Abundance..127N":19058.08443,"Abundance..127C":14764.26419,"Abundance..128N":20513.67122,"Abundance..128C":13603.38615,"Abundance..129N":14559.85934,"Abundance..129C":19467.48189,"Abundance..130N":21900.22697,"Abundance..130C":12691.44047,"Abundance..131":19068.37548,"Ions.Score":80,"Identity.Strict":24,"Identity.Relaxed":17,"Expectation.Value":3.10026e-08,"Percolator.q.Value":0,"Percolator.PEP":2.298e-09}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (O2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[R].dkPSVEPVEEYDYEDLk.[E]","Modifications":"N-Term(TMT6plex); K2(Label); K17(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q9Y450","Master.Protein.Descriptions":"HBS1-like protein OS=Homo sapiens GN=HBS1L PE=1 SV=1","Protein.Accessions":"Q9Y450","Protein.Descriptions":"HBS1-like protein OS=Homo sapiens GN=HBS1L PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"DeltaScore":0.9583,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":920.15918,"MH...Da.":2758.46299,"Theo..MH...Da.":2758.46127,"DeltaM..ppm.":0.62,"Deltam.z..Da.":0.00057,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":50.85299,"Average.Reporter.S.N":25,"Ion.Inject.Time..ms.":23.566,"RT..min.":143.4421,"First.Scan":73704,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_03.raw","File.ID":"F1","Abundance..126":24741.69154,"Abundance..127N":26598.82504,"Abundance..127C":24142.39806,"Abundance..128N":29060.84584,"Abundance..128C":29334.78926,"Abundance..129N":26869.40759,"Abundance..129C":31946.26871,"Abundance..130N":31612.2697,"Abundance..130C":33459.09923,"Abundance..131":30133.07747,"Ions.Score":72,"Identity.Strict":30,"Identity.Relaxed":23,"Expectation.Value":6.81273e-07,"Percolator.q.Value":0,"Percolator.PEP":5.523e-09}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (O2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].dNLTLWTADNAGEEGGEAPQEPQS.[-]","Modifications":"N-Term(TMT6plex)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P31947","Master.Protein.Descriptions":"14-3-3 protein sigma OS=Homo sapiens GN=SFN PE=1 SV=1","Protein.Accessions":"P31947","Protein.Descriptions":"14-3-3 protein sigma OS=Homo sapiens GN=SFN PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":920.09308,"MH...Da.":2758.26468,"Theo..MH...Da.":2758.26413,"DeltaM..ppm.":0.2,"Deltam.z..Da.":0.00019,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":16.16386,"Average.Reporter.S.N":38.1,"Ion.Inject.Time..ms.":7.079,"RT..min.":174.2758,"First.Scan":91636,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_03.raw","File.ID":"F1","Abundance..126":116296.88995,"Abundance..127N":104033.19425,"Abundance..127C":109658.12893,"Abundance..128N":123216.27443,"Abundance..128C":98959.78003,"Abundance..129N":97624.47741,"Abundance..129C":111710.98455,"Abundance..130N":112076.35094,"Abundance..130C":103135.973,"Abundance..131":106075.57362,"Ions.Score":34,"Identity.Strict":20,"Identity.Relaxed":13,"Expectation.Value":0.00045,"Percolator.q.Value":0,"Percolator.PEP":2.323e-06}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (O2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].wGDAGAEYVVESTGVFTTmEk.[A]","Modifications":"N-Term(TMT6plex); M19(Oxidation); K21(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":920.4646,"MH...Da.":2759.37925,"Theo..MH...Da.":2759.37297,"DeltaM..ppm.":2.28,"Deltam.z..Da.":0.00209,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":24.53713,"Average.Reporter.S.N":14.2,"Ion.Inject.Time..ms.":16.348,"RT..min.":185.4375,"First.Scan":98099,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_03.raw","File.ID":"F1","Abundance..126":8361.10285,"Abundance..127N":6231.08503,"Abundance..127C":6103.64775,"Abundance..128N":6908.34149,"Abundance..128C":10407.83766,"Abundance..129N":6309.78591,"Abundance..129C":8658.16807,"Abundance..130N":8372.21068,"Abundance..130C":7147.11987,"Abundance..131":8436.84255,"Ions.Score":34,"Identity.Strict":26,"Identity.Relaxed":19,"Expectation.Value":0.00154,"Percolator.q.Value":0,"Percolator.PEP":0.00011}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (O2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].drLPLAVVGSNTIIEVNGk.[R]","Modifications":"N-Term(TMT6plex); R2(Label:13C(6)15N(4)); K19(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q16181","Master.Protein.Descriptions":"Septin-7 OS=Homo sapiens GN=SEPT7 PE=1 SV=2","Protein.Accessions":"Q16181","Protein.Descriptions":"Septin-7 OS=Homo sapiens GN=SEPT7 PE=1 SV=2","X..Missed.Cleavages":1,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":824.49817,"MH...Da.":2471.47995,"Theo..MH...Da.":2471.47165,"DeltaM..ppm.":3.36,"Deltam.z..Da.":0.00277,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":52.53085,"Average.Reporter.S.N":18.3,"Ion.Inject.Time..ms.":6.178,"RT..min.":157.6244,"First.Scan":81961,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_03.raw","File.ID":"F1","Abundance..126":17305.39045,"Abundance..127N":18424.92141,"Abundance..127C":25136.46064,"Abundance..128N":24443.13587,"Abundance..128C":27749.74786,"Abundance..129N":24338.48961,"Abundance..129C":22056.44315,"Abundance..130N":22401.65583,"Abundance..130C":22839.86747,"Abundance..131":27809.47477,"Ions.Score":37,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":0.0012,"Percolator.q.Value":0,"Percolator.PEP":0.00002}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (O2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].iPSAVGYQPTLATDMGTMQEr.[I]","Modifications":"N-Term(TMT6plex); R21(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":835.75787,"MH...Da.":2505.25907,"Theo..MH...Da.":2505.25543,"DeltaM..ppm.":1.45,"Deltam.z..Da.":0.00121,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":50.22768,"Average.Reporter.S.N":15.1,"Ion.Inject.Time..ms.":1.391,"RT..min.":162.2538,"First.Scan":84675,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_03.raw","File.ID":"F1","Abundance..126":174831.15501,"Abundance..127N":132729.93411,"Abundance..127C":151391.24432,"Abundance..128N":178211.11016,"Abundance..128C":107740.69069,"Abundance..129N":167918.57859,"Abundance..129C":116147.30536,"Abundance..130N":121595.95761,"Abundance..130C":140159.37244,"Abundance..131":209700.77153,"Ions.Score":65,"Identity.Strict":26,"Identity.Relaxed":19,"Expectation.Value":1.23533e-06,"Percolator.q.Value":0,"Percolator.PEP":2.852e-07}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (O2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].fLSQPFQVAEVFTGHMGk.[L]","Modifications":"N-Term(TMT6plex); K18(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":830.45538,"MH...Da.":2489.3516,"Theo..MH...Da.":2489.35066,"DeltaM..ppm.":0.38,"Deltam.z..Da.":0.00031,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":11.48634,"Average.Reporter.S.N":47.2,"Ion.Inject.Time..ms.":3.625,"RT..min.":186.3914,"First.Scan":98636,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_03.raw","File.ID":"F1","Abundance..126":359981.8736,"Abundance..127N":334093.95293,"Abundance..127C":311613.77029,"Abundance..128N":407944.08274,"Abundance..128C":337553.40686,"Abundance..129N":408088.62546,"Abundance..129C":345639.40589,"Abundance..130N":368309.69157,"Abundance..130C":387087.16928,"Abundance..131":375319.23285,"Ions.Score":55,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.00003,"Percolator.q.Value":0,"Percolator.PEP":6.346e-10}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (O2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].iPSAVGYQPTLATDmGTMQEr.[I]","Modifications":"N-Term(TMT6plex); M15(Oxidation); R21(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.2273,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":841.08942,"MH...Da.":2521.2537,"Theo..MH...Da.":2521.25034,"DeltaM..ppm.":1.33,"Deltam.z..Da.":0.00112,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":41.3675,"Average.Reporter.S.N":20.1,"Ion.Inject.Time..ms.":12.398,"RT..min.":148.3566,"First.Scan":76572,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_03.raw","File.ID":"F1","Abundance..126":32688.96066,"Abundance..127N":42920.80411,"Abundance..127C":39261.6543,"Abundance..128N":53043.43827,"Abundance..128C":43552.08363,"Abundance..129N":37308.64764,"Abundance..129C":49521.84201,"Abundance..130N":41414.74745,"Abundance..130C":42402.94093,"Abundance..131":46438.24555,"Ions.Score":44,"Identity.Strict":25,"Identity.Relaxed":18,"Expectation.Value":0.00013,"Percolator.q.Value":0,"Percolator.PEP":0.00004}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (O2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].dYEFMWNPHLGYILTcPSNLGTGLr.[A]","Modifications":"N-Term(TMT6plex); C16(Carbamidomethyl); R25(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P12277","Master.Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","Protein.Accessions":"P12277","Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":1065.19507,"MH...Da.":3193.57065,"Theo..MH...Da.":3193.5676,"DeltaM..ppm.":0.96,"Deltam.z..Da.":0.00102,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":5.98157,"Average.Reporter.S.N":21.2,"Ion.Inject.Time..ms.":6.541,"RT..min.":190.4975,"First.Scan":100957,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_03.raw","File.ID":"F1","Abundance..126":130541.14873,"Abundance..127N":123956.06677,"Abundance..127C":92807.59994,"Abundance..128N":122477.82713,"Abundance..128C":114815.91843,"Abundance..129N":114295.08317,"Abundance..129C":114579.02959,"Abundance..130N":145818.86377,"Abundance..130C":109102.10995,"Abundance..131":118844.51823,"Ions.Score":59,"Identity.Strict":26,"Identity.Relaxed":19,"Expectation.Value":5.47139e-06,"Percolator.q.Value":0,"Percolator.PEP":1.651e-08}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (A2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].dQEGQDVLLFIDNIFr.[F]","Modifications":"N-Term(TMT6plex); R16(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":1081.07361,"MH...Da.":2161.13994,"Theo..MH...Da.":2161.13662,"DeltaM..ppm.":1.54,"Deltam.z..Da.":0.00166,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":0,"Average.Reporter.S.N":7,"Ion.Inject.Time..ms.":50,"RT..min.":234.7962,"First.Scan":119979,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_01.raw","File.ID":"F15","Abundance..126":2046.67575,"Abundance..127N":2425.85313,"Abundance..127C":2418.78072,"Abundance..128N":2086.73287,"Abundance..128C":2970.59011,"Abundance..129N":2523.11091,"Abundance..129C":3564.45026,"Abundance..130N":2567.47485,"Abundance..130C":1498.87994,"Abundance..131":1444.03189,"Ions.Score":108,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":1.1116e-10,"Percolator.q.Value":0,"Percolator.PEP":1.706e-11}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (A2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[K].sLQDIIAILGMDELSEEDk.[L]","Modifications":"N-Term(TMT6plex); K19(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":862.46863,"MH...Da.":2585.39133,"Theo..MH...Da.":2585.38755,"DeltaM..ppm.":1.46,"Deltam.z..Da.":0.00126,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":17.23181,"Average.Reporter.S.N":15.9,"Ion.Inject.Time..ms.":50,"RT..min.":235.7066,"First.Scan":120393,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_01.raw","File.ID":"F15","Abundance..126":4379.44981,"Abundance..127N":5718.9156,"Abundance..127C":5827.86606,"Abundance..128N":5243.44475,"Abundance..128C":4556.98804,"Abundance..129N":5517.48282,"Abundance..129C":5845.63964,"Abundance..130N":6744.78021,"Abundance..130C":5711.40539,"Abundance..131":4789.77731,"Ions.Score":49,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.00012,"Percolator.q.Value":0,"Percolator.PEP":4.882e-08}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (A2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].sLQDIIAILGMDELSEEDk.[L]","Modifications":"N-Term(TMT6plex); K19(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":862.46796,"MH...Da.":2585.38932,"Theo..MH...Da.":2585.38755,"DeltaM..ppm.":0.68,"Deltam.z..Da.":0.00059,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":11.25795,"Average.Reporter.S.N":4.6,"Ion.Inject.Time..ms.":50,"RT..min.":237.0447,"First.Scan":120983,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_01.raw","File.ID":"F15","Abundance..126":941.41338,"Abundance..127N":462.59253,"Abundance..127C":1666.09418,"Abundance..128N":2241.04763,"Abundance..128C":1481.51527,"Abundance..129N":1721.78678,"Abundance..129C":2152.58462,"Abundance..130N":1572.93144,"Abundance..130C":1086.94708,"Abundance..131":2640.63495,"Ions.Score":23,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.04208,"Percolator.q.Value":0,"Percolator.PEP":0.00009}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (A2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].sLQDIIAILGmDELSEEDk.[L]","Modifications":"N-Term(TMT6plex); M11(Oxidation); K19(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":867.80035,"MH...Da.":2601.38651,"Theo..MH...Da.":2601.38247,"DeltaM..ppm.":1.55,"Deltam.z..Da.":0.00135,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":24.12697,"Average.Reporter.S.N":44.1,"Ion.Inject.Time..ms.":33.004,"RT..min.":224.1022,"First.Scan":115139,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_01.raw","File.ID":"F15","Abundance..126":20349.03794,"Abundance..127N":25411.92819,"Abundance..127C":20633.52556,"Abundance..128N":18920.4736,"Abundance..128C":27325.09743,"Abundance..129N":26314.7576,"Abundance..129C":24391.73126,"Abundance..130N":25709.54401,"Abundance..130C":23134.35441,"Abundance..131":24015.95706,"Ions.Score":28,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.01717,"Percolator.q.Value":0,"Percolator.PEP":3.893e-07}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (O2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[R].yEDMAAFMk.[G]","Modifications":"N-Term(TMT6plex); K9(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P31947","Master.Protein.Descriptions":"14-3-3 protein sigma OS=Homo sapiens GN=SFN PE=1 SV=1","Protein.Accessions":"P31947","Protein.Descriptions":"14-3-3 protein sigma OS=Homo sapiens GN=SFN PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":0.8056,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":786.40814,"MH...Da.":1571.80901,"Theo..MH...Da.":1571.80934,"DeltaM..ppm.":-0.21,"Deltam.z..Da.":-0.00017,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":37.67072,"Average.Reporter.S.N":32.8,"Ion.Inject.Time..ms.":5.658,"RT..min.":157.9611,"First.Scan":82158,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_03.raw","File.ID":"F1","Abundance..126":85273.48411,"Abundance..127N":91580.27367,"Abundance..127C":86977.21048,"Abundance..128N":94740.51135,"Abundance..128C":82551.94253,"Abundance..129N":98891.88222,"Abundance..129C":82348.62201,"Abundance..130N":94634.1973,"Abundance..130C":99961.13986,"Abundance..131":107756.06323,"Ions.Score":36,"Identity.Strict":27,"Identity.Relaxed":20,"Expectation.Value":0.00145,"Percolator.q.Value":0,"Percolator.PEP":0.00025}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (A2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].hDMAAAGFTEALTFALcSQEDIADk.[L]","Modifications":"N-Term(TMT6plex); C17(Carbamidomethyl); K25(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q9NSD9","Master.Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","Protein.Accessions":"Q9NSD9","Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":1060.19482,"MH...Da.":3178.56992,"Theo..MH...Da.":3178.56806,"DeltaM..ppm.":0.59,"Deltam.z..Da.":0.00062,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":55.73323,"Average.Reporter.S.N":9,"Ion.Inject.Time..ms.":24.006,"RT..min.":201.0758,"First.Scan":103953,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_01.raw","File.ID":"F15","Abundance..126":3171.95708,"Abundance..127N":1860.73844,"Abundance..127C":2733.31622,"Abundance..128N":2196.04908,"Abundance..128C":2141.38935,"Abundance..129N":4289.75412,"Abundance..129C":2414.60386,"Abundance..130N":3090.56232,"Abundance..130C":4337.47276,"Abundance..131":4164.56747,"Ions.Score":45,"Identity.Strict":25,"Identity.Relaxed":18,"Expectation.Value":0.00013,"Percolator.q.Value":0,"Percolator.PEP":0.00004}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (A2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].vIHDNFGIVEGLMTTVHAITATQk.[T]","Modifications":"N-Term(TMT6plex); K24(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":0,"Charge":4,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":766.18134,"MH...Da.":3061.70351,"Theo..MH...Da.":3061.7,"DeltaM..ppm.":1.15,"Deltam.z..Da.":0.00088,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":5.79405,"Average.Reporter.S.N":45.8,"Ion.Inject.Time..ms.":1.856,"RT..min.":194.7985,"First.Scan":100641,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_01.raw","File.ID":"F15","Abundance..126":378309.60752,"Abundance..127N":373292.87165,"Abundance..127C":392683.41227,"Abundance..128N":369830.9168,"Abundance..128C":389054.98002,"Abundance..129N":375198.50387,"Abundance..129C":411101.06373,"Abundance..130N":459157.27934,"Abundance..130C":394646.70168,"Abundance..131":399334.8201,"Ions.Score":44,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.00036,"Percolator.q.Value":0,"Percolator.PEP":1.964e-06}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (A2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].vIHDNFGIVEGLMTTVHAITATQk.[T]","Modifications":"N-Term(TMT6plex); K24(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":0,"Charge":4,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":766.18231,"MH...Da.":3061.70742,"Theo..MH...Da.":3061.7,"DeltaM..ppm.":2.42,"Deltam.z..Da.":0.00186,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":29.32174,"Average.Reporter.S.N":17.9,"Ion.Inject.Time..ms.":23.731,"RT..min.":196.943,"First.Scan":101782,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_01.raw","File.ID":"F15","Abundance..126":22944.57169,"Abundance..127N":22262.60117,"Abundance..127C":18188.11759,"Abundance..128N":23221.39189,"Abundance..128C":23849.95034,"Abundance..129N":23810.86215,"Abundance..129C":22708.77933,"Abundance..130N":30780.02537,"Abundance..130C":27860.41609,"Abundance..131":26624.94761,"Ions.Score":22,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.05699,"Percolator.q.Value":0,"Percolator.PEP":0.00001}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (A2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].dYEFMWNPHLGYILTcPSNLGTGLr.[A]","Modifications":"N-Term(TMT6plex); C16(Carbamidomethyl); R25(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P12277","Master.Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","Protein.Accessions":"P12277","Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":1065.19519,"MH...Da.":3193.57102,"Theo..MH...Da.":3193.5676,"DeltaM..ppm.":1.07,"Deltam.z..Da.":0.00114,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":0,"Average.Reporter.S.N":0.7,"Ion.Inject.Time..ms.":7.742,"RT..min.":192.0003,"First.Scan":99149,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_01.raw","File.ID":"F15","Abundance..129N":16827.92497,"Abundance..130N":28717.83604,"Abundance..130C":30841.41602,"Ions.Score":70,"Identity.Strict":26,"Identity.Relaxed":19,"Expectation.Value":5.22514e-07,"Percolator.q.Value":0,"Percolator.PEP":3.357e-09}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (A2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].rVIISAPSADAPmFVMGVNHEk.[Y]","Modifications":"N-Term(TMT6plex); R1(Label:13C(6)15N(4)); M13(Oxidation); K22(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":1,"Charge":4,"DeltaScore":0.1897,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":716.14484,"MH...Da.":2861.55752,"Theo..MH...Da.":2861.55368,"DeltaM..ppm.":1.34,"Deltam.z..Da.":0.00096,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":31.88717,"Average.Reporter.S.N":21.6,"Ion.Inject.Time..ms.":3.339,"RT..min.":133.7756,"First.Scan":66277,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_01.raw","File.ID":"F15","Abundance..126":84323.48512,"Abundance..127N":86996.56899,"Abundance..127C":46995.68508,"Abundance..128N":85605.26761,"Abundance..128C":73557.48979,"Abundance..129N":80810.81773,"Abundance..129C":70930.59645,"Abundance..130N":81214.16311,"Abundance..130C":69478.92897,"Abundance..131":79013.82041,"Ions.Score":58,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.00002,"Percolator.q.Value":0,"Percolator.PEP":4.123e-10}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (A2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].rVIISAPSADAPMFVmGVNHEk.[Y]","Modifications":"N-Term(TMT6plex); R1(Label:13C(6)15N(4)); M16(Oxidation); K22(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":1,"Charge":4,"DeltaScore":0.4038,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":716.14435,"MH...Da.":2861.55556,"Theo..MH...Da.":2861.55368,"DeltaM..ppm.":0.66,"Deltam.z..Da.":0.00047,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":3.63973,"Average.Reporter.S.N":22.1,"Ion.Inject.Time..ms.":1.217,"RT..min.":132.3596,"First.Scan":65466,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_01.raw","File.ID":"F15","Abundance..126":130536.40374,"Abundance..127N":103636.8723,"Abundance..127C":87137.26513,"Abundance..128N":94038.61307,"Abundance..128C":111713.46929,"Abundance..129N":104849.77846,"Abundance..129C":97937.27954,"Abundance..130N":112286.85236,"Abundance..130C":101765.06096,"Abundance..131":140439.89151,"Ions.Score":52,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.00006,"Percolator.q.Value":0,"Percolator.PEP":5.504e-07}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (A2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].qFAPIHAEAPEFMEMSVEQEILVTGIk.[V]","Modifications":"N-Term(TMT6plex); K27(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":4,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":878.46735,"MH...Da.":3510.84755,"Theo..MH...Da.":3510.85082,"DeltaM..ppm.":-0.93,"Deltam.z..Da.":-0.00082,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":0,"Average.Reporter.S.N":18.1,"Ion.Inject.Time..ms.":6.398,"RT..min.":198.9175,"First.Scan":102826,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_01.raw","File.ID":"F15","Abundance..126":28447.00101,"Abundance..127N":21531.76331,"Abundance..127C":20072.50818,"Abundance..128N":32566.41934,"Abundance..128C":30955.68723,"Abundance..129N":24766.44157,"Abundance..129C":26872.92794,"Abundance..130N":29254.20598,"Abundance..130C":27031.26782,"Abundance..131":20043.69812,"Ions.Score":32,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.0058,"Percolator.q.Value":0,"Percolator.PEP":2.588e-06}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (O2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].irPFAVAAVLr.[N]","Modifications":"N-Term(TMT6plex); R2(Label:13C(6)15N(4)); R11(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q9NSD9","Master.Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","Protein.Accessions":"Q9NSD9","Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","X..Missed.Cleavages":1,"Charge":3,"DeltaScore":0.931,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":487.98395,"MH...Da.":1461.93729,"Theo..MH...Da.":1461.93701,"DeltaM..ppm.":0.19,"Deltam.z..Da.":0.00009,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":10.51127,"Average.Reporter.S.N":39.8,"Ion.Inject.Time..ms.":2.719,"RT..min.":134.3923,"First.Scan":68448,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_03.raw","File.ID":"F1","Abundance..126":154164.95221,"Abundance..127N":164229.27497,"Abundance..127C":175829.04377,"Abundance..128N":188048.39125,"Abundance..128C":207992.33675,"Abundance..129N":179417.21244,"Abundance..129C":174153.51335,"Abundance..130N":207261.80357,"Abundance..130C":175567.78756,"Abundance..131":188275.8088,"Ions.Score":29,"Identity.Strict":27,"Identity.Relaxed":20,"Expectation.Value":0.00727,"Percolator.q.Value":0,"Percolator.PEP":0.00009}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (A4)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].vPTANVSVVDLTcR.[L]","Modifications":"N-Term(TMT6plex); C13(Carbamidomethyl)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":587.3255,"MH...Da.":1759.96195,"Theo..MH...Da.":1759.95739,"DeltaM..ppm.":2.59,"Deltam.z..Da.":0.00152,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":12.92029,"Average.Reporter.S.N":37.6,"Ion.Inject.Time..ms.":7.58,"RT..min.":147.9867,"First.Scan":74433,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_01.raw","File.ID":"F15","Abundance..126":77674.24954,"Abundance..127N":75232.7549,"Abundance..127C":65614.15048,"Abundance..128N":69324.06628,"Abundance..128C":58398.5898,"Abundance..129N":73557.99812,"Abundance..129C":72111.21555,"Abundance..130N":72200.83905,"Abundance..130C":70616.8259,"Abundance..131":62698.69171,"Ions.Score":44,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.00036,"Percolator.q.Value":0,"Percolator.PEP":0.00909}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (A4)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[K].tVLIMELINNVAk.[A]","Modifications":"N-Term(TMT6plex); K13(TMT6plex)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.8125,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":639.39398,"MH...Da.":1916.16739,"Theo..MH...Da.":1916.16548,"DeltaM..ppm.":1,"Deltam.z..Da.":0.00064,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":0,"Average.Reporter.S.N":33.4,"Ion.Inject.Time..ms.":50,"RT..min.":212.019,"First.Scan":109410,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_01.raw","File.ID":"F15","Abundance..126":12080.26087,"Abundance..127N":13710.62753,"Abundance..127C":8895.28411,"Abundance..128N":10373.05426,"Abundance..128C":12942.29456,"Abundance..129N":12862.0746,"Abundance..129C":12166.06031,"Abundance..130N":10938.7004,"Abundance..130C":12377.1019,"Abundance..131":11942.96807,"Ions.Score":32,"Identity.Strict":32,"Identity.Relaxed":25,"Expectation.Value":0.01076,"Percolator.q.Value":0,"Percolator.PEP":0.03848}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (A2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].lISWYDNEFGYSNr.[V]","Modifications":"N-Term(TMT6plex); R14(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":668.33014,"MH...Da.":2002.97586,"Theo..MH...Da.":2002.97358,"DeltaM..ppm.":1.14,"Deltam.z..Da.":0.00076,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":27.46349,"Average.Reporter.S.N":58.9,"Ion.Inject.Time..ms.":5.097,"RT..min.":178.458,"First.Scan":91744,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_01.raw","File.ID":"F15","Abundance..126":200189.42437,"Abundance..127N":196008.26521,"Abundance..127C":191578.62478,"Abundance..128N":208072.99971,"Abundance..128C":205919.61287,"Abundance..129N":200735.08992,"Abundance..129C":234883.08444,"Abundance..130N":220603.85096,"Abundance..130C":171347.39508,"Abundance..131":200087.6319,"Ions.Score":40,"Identity.Strict":24,"Identity.Relaxed":17,"Expectation.Value":0.00027,"Percolator.q.Value":0,"Percolator.PEP":4.451e-06}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (A2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[R].iMDPNIVGSEHYDVAr.[G]","Modifications":"N-Term(TMT6plex); R16(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":685.68573,"MH...Da.":2055.04264,"Theo..MH...Da.":2055.04061,"DeltaM..ppm.":0.99,"Deltam.z..Da.":0.00068,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":7.29736,"Average.Reporter.S.N":31,"Ion.Inject.Time..ms.":7.15,"RT..min.":131.5735,"First.Scan":65024,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_01.raw","File.ID":"F15","Abundance..126":56194.06527,"Abundance..127N":64410.28184,"Abundance..127C":73771.30354,"Abundance..128N":93194.06073,"Abundance..128C":71330.88426,"Abundance..129N":62099.07352,"Abundance..129C":69330.75373,"Abundance..130N":80974.21569,"Abundance..130C":93876.03807,"Abundance..131":78639.22982,"Ions.Score":47,"Identity.Strict":26,"Identity.Relaxed":19,"Expectation.Value":0.00008,"Percolator.q.Value":0,"Percolator.PEP":1.245e-06}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (A2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].lFVGNLPPDITEEEMr.[K]","Modifications":"N-Term(TMT6plex); R16(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q15233","Master.Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","Protein.Accessions":"Q15233","Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":1050.05017,"MH...Da.":2099.09307,"Theo..MH...Da.":2099.09198,"DeltaM..ppm.":0.52,"Deltam.z..Da.":0.00054,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":31.57213,"Average.Reporter.S.N":19.5,"Ion.Inject.Time..ms.":19.166,"RT..min.":179.4367,"First.Scan":92300,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_01.raw","File.ID":"F15","Abundance..126":13481.45835,"Abundance..127N":11755.00122,"Abundance..127C":7756.18338,"Abundance..128N":9494.00444,"Abundance..128C":12183.63752,"Abundance..129N":13355.98045,"Abundance..129C":14600.45713,"Abundance..130N":10308.81519,"Abundance..130C":11967.7597,"Abundance..131":11809.56913,"Ions.Score":22,"Identity.Strict":27,"Identity.Relaxed":20,"Expectation.Value":0.03995,"Percolator.q.Value":0,"Percolator.PEP":0.0001}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (M2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].fLSQPFQVAEVFTGHMGk.[L]","Modifications":"N-Term(TMT6plex); K18(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":830.45618,"MH...Da.":2489.35398,"Theo..MH...Da.":2489.35066,"DeltaM..ppm.":1.33,"Deltam.z..Da.":0.00111,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":10.51343,"Average.Reporter.S.N":50.5,"Ion.Inject.Time..ms.":7.934,"RT..min.":186.337,"First.Scan":95118,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_03.raw","File.ID":"F3","Abundance..126":73485.98934,"Abundance..127N":68887.55025,"Abundance..127C":77607.18796,"Abundance..128N":64916.14499,"Abundance..128C":75209.80359,"Abundance..129N":68169.91783,"Abundance..129C":75052.57956,"Abundance..130N":79557.11792,"Abundance..130C":78280.18004,"Abundance..131":75169.23419,"Ions.Score":64,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":3.55208e-06,"Percolator.q.Value":0,"Percolator.PEP":3.771e-09}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (M2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].iMNVIGEPIDEr.[G]","Modifications":"N-Term(TMT6plex); R12(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":0.9342,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":812.94373,"MH...Da.":1624.88017,"Theo..MH...Da.":1624.88053,"DeltaM..ppm.":-0.22,"Deltam.z..Da.":-0.00018,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":18.27109,"Average.Reporter.S.N":31.9,"Ion.Inject.Time..ms.":9.401,"RT..min.":154.5592,"First.Scan":77071,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_03.raw","File.ID":"F3","Abundance..126":55246.65365,"Abundance..127N":54626.58936,"Abundance..127C":56230.54893,"Abundance..128N":58726.32725,"Abundance..128C":63261.84615,"Abundance..129N":61569.09171,"Abundance..129C":64862.50208,"Abundance..130N":55257.38009,"Abundance..130C":71785.67476,"Abundance..131":55673.27401,"Ions.Score":76,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":1.79611e-07,"Percolator.q.Value":0,"Percolator.PEP":0.00004}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (M2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].yLAEVATGDDk.[K]","Modifications":"N-Term(TMT6plex); K11(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P31947","Master.Protein.Descriptions":"14-3-3 protein sigma OS=Homo sapiens GN=SFN PE=1 SV=1","Protein.Accessions":"P31947","Protein.Descriptions":"14-3-3 protein sigma OS=Homo sapiens GN=SFN PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":824.45996,"MH...Da.":1647.91265,"Theo..MH...Da.":1647.90852,"DeltaM..ppm.":2.5,"Deltam.z..Da.":0.00206,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":13.46263,"Average.Reporter.S.N":39.2,"Ion.Inject.Time..ms.":9.182,"RT..min.":126.3754,"First.Scan":61117,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_03.raw","File.ID":"F3","Abundance..126":19456.44144,"Abundance..127N":18810.52162,"Abundance..127C":22060.99594,"Abundance..128N":23883.63743,"Abundance..128C":19619.42616,"Abundance..129N":21803.67259,"Abundance..129C":19811.74813,"Abundance..130N":20862.64563,"Abundance..130C":23109.16144,"Abundance..131":21895.24915,"Ions.Score":38,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":0.00129,"Percolator.q.Value":0,"Percolator.PEP":0.00004}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (C2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].vPTANVSVVDLTcr.[L]","Modifications":"N-Term(TMT6plex); C13(Carbamidomethyl); R14(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":0.8814,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":885.48718,"MH...Da.":1769.96709,"Theo..MH...Da.":1769.96565,"DeltaM..ppm.":0.81,"Deltam.z..Da.":0.00072,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":34.23216,"Average.Reporter.S.N":8.4,"Ion.Inject.Time..ms.":4.786,"RT..min.":147.5267,"First.Scan":75012,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture4_01.raw","File.ID":"F13","Abundance..126":143287.76529,"Abundance..127N":94968.35476,"Abundance..127C":92146.18801,"Abundance..128N":143261.59654,"Abundance..128C":91485.1714,"Abundance..129N":136161.39303,"Abundance..129C":113498.69151,"Abundance..130N":89670.66474,"Abundance..130C":123284.90391,"Abundance..131":110576.4061,"Ions.Score":59,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.00001,"Percolator.q.Value":0,"Percolator.PEP":0.00001}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (M2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].iPSAVGYQPTLATDMGTMQEr.[I]","Modifications":"N-Term(TMT6plex); R21(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":835.75769,"MH...Da.":2505.25852,"Theo..MH...Da.":2505.25543,"DeltaM..ppm.":1.23,"Deltam.z..Da.":0.00103,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":1.00442,"Average.Reporter.S.N":13.1,"Ion.Inject.Time..ms.":1.279,"RT..min.":161.878,"First.Scan":81291,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_03.raw","File.ID":"F3","Abundance..126":90997.36569,"Abundance..127N":62091.41269,"Abundance..127C":74298.93891,"Abundance..128N":114472.82173,"Abundance..128C":105261.27277,"Abundance..129N":75646.75166,"Abundance..129C":91168.58198,"Abundance..130N":97672.42582,"Abundance..130C":104427.97091,"Abundance..131":116587.91809,"Ions.Score":68,"Identity.Strict":26,"Identity.Relaxed":19,"Expectation.Value":6.6229e-07,"Percolator.q.Value":0,"Percolator.PEP":1.428e-08}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (M2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[R].fLSQPFQVAEVFTGHmGk.[L]","Modifications":"N-Term(TMT6plex); M16(Oxidation); K18(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":835.78839,"MH...Da.":2505.35062,"Theo..MH...Da.":2505.34557,"DeltaM..ppm.":2.02,"Deltam.z..Da.":0.00168,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":13.13942,"Average.Reporter.S.N":7.9,"Ion.Inject.Time..ms.":4.109,"RT..min.":175.9995,"First.Scan":89350,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_03.raw","File.ID":"F3","Abundance..126":9409.69558,"Abundance..127N":11272.23183,"Abundance..127C":13903.31007,"Abundance..128N":14954.40982,"Abundance..128C":12625.7531,"Abundance..129N":20647.37193,"Abundance..129C":12148.33023,"Abundance..130N":15812.96269,"Abundance..130C":21617.19208,"Abundance..131":16886.40474,"Ions.Score":44,"Identity.Strict":30,"Identity.Relaxed":23,"Expectation.Value":0.00037,"Percolator.q.Value":0,"Percolator.PEP":1.8e-06}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (M2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].lVINGNPITIFQErDPSk.[I]","Modifications":"N-Term(TMT6plex); R14(Label:13C(6)15N(4)); K18(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":1,"Charge":3,"DeltaScore":0.9091,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":839.8241,"MH...Da.":2517.45774,"Theo..MH...Da.":2517.456,"DeltaM..ppm.":0.69,"Deltam.z..Da.":0.00058,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":5.03024,"Average.Reporter.S.N":44.5,"Ion.Inject.Time..ms.":6.622,"RT..min.":165.7275,"First.Scan":83510,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_03.raw","File.ID":"F3","Abundance..126":134584.78966,"Abundance..127N":146968.45548,"Abundance..127C":124551.53912,"Abundance..128N":112244.72165,"Abundance..128C":127435.46503,"Abundance..129N":116769.03159,"Abundance..129C":112403.14081,"Abundance..130N":130599.17681,"Abundance..130C":136865.59824,"Abundance..131":108782.91175,"Ions.Score":55,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.00003,"Percolator.q.Value":0,"Percolator.PEP":2.025e-06}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (M2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].tFLVWVNEEDHLr.[V]","Modifications":"N-Term(TMT6plex); R13(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P12277","Master.Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","Protein.Accessions":"P12277","Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":633.00647,"MH...Da.":1897.00486,"Theo..MH...Da.":1897.00448,"DeltaM..ppm.":0.2,"Deltam.z..Da.":0.00012,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":26.88468,"Average.Reporter.S.N":27.8,"Ion.Inject.Time..ms.":2.661,"RT..min.":160.3876,"First.Scan":80417,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_03.raw","File.ID":"F3","Abundance..126":136105.07484,"Abundance..127N":136808.5513,"Abundance..127C":117354.39893,"Abundance..128N":117378.88904,"Abundance..128C":120328.30314,"Abundance..129N":132234.9184,"Abundance..129C":119408.79519,"Abundance..130N":139600.52105,"Abundance..130C":165819.03627,"Abundance..131":143760.24911,"Ions.Score":42,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.00055,"Percolator.q.Value":0,"Percolator.PEP":0.00001}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (C2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].rGTGGVDTAAVGGVFDVSNADr.[L]","Modifications":"N-Term(TMT6plex); R1(Label:13C(6)15N(4)); R22(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P12277","Master.Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","Protein.Accessions":"P12277","Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":790.74243,"MH...Da.":2370.21274,"Theo..MH...Da.":2370.2114,"DeltaM..ppm.":0.56,"Deltam.z..Da.":0.00045,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":13.97378,"Average.Reporter.S.N":32.7,"Ion.Inject.Time..ms.":9.321,"RT..min.":121.5527,"First.Scan":60454,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture4_01.raw","File.ID":"F13","Abundance..126":69957.42069,"Abundance..127N":69801.28657,"Abundance..127C":70585.82962,"Abundance..128N":75543.67277,"Abundance..128C":71598.13447,"Abundance..129N":74797.88343,"Abundance..129C":73036.67147,"Abundance..130N":60185.65923,"Abundance..130C":73626.55837,"Abundance..131":78378.98329,"Ions.Score":77,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":1.23829e-07,"Percolator.q.Value":0,"Percolator.PEP":1.98e-07}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (C2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].lIMVLAGASEFDPQYNk.[D]","Modifications":"N-Term(TMT6plex); K17(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q9UGP8","Master.Protein.Descriptions":"Translocation protein SEC63 homolog OS=Homo sapiens GN=SEC63 PE=1 SV=2","Protein.Accessions":"Q9UGP8","Protein.Descriptions":"Translocation protein SEC63 homolog OS=Homo sapiens GN=SEC63 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":788.10443,"MH...Da.":2362.29874,"Theo..MH...Da.":2362.29722,"DeltaM..ppm.":0.64,"Deltam.z..Da.":0.00051,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":9.35818,"Average.Reporter.S.N":25.1,"Ion.Inject.Time..ms.":13.313,"RT..min.":197.6637,"First.Scan":103104,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture4_01.raw","File.ID":"F13","Abundance..126":15689.94637,"Abundance..127N":18187.43131,"Abundance..127C":17592.97512,"Abundance..128N":21508.89925,"Abundance..128C":20780.91784,"Abundance..129N":15106.8938,"Abundance..129C":21908.60798,"Abundance..130N":17404.06244,"Abundance..130C":18142.16385,"Abundance..131":17910.81734,"Ions.Score":7,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":1.78239,"Percolator.q.Value":0,"Percolator.PEP":0.00012}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (M2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].vALVYGQmNEPPGAr.[A]","Modifications":"N-Term(TMT6plex); M8(Oxidation); R15(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.9474,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":619.66382,"MH...Da.":1856.9769,"Theo..MH...Da.":1856.97655,"DeltaM..ppm.":0.19,"Deltam.z..Da.":0.00012,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":64.59465,"Average.Reporter.S.N":18.2,"Ion.Inject.Time..ms.":21.812,"RT..min.":116.6467,"First.Scan":55725,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_03.raw","File.ID":"F3","Abundance..126":9974.06145,"Abundance..127N":15185.49656,"Abundance..127C":12793.96614,"Abundance..128N":13437.32848,"Abundance..128C":15647.72748,"Abundance..129N":18399.93388,"Abundance..129C":15724.44199,"Abundance..130N":12645.32345,"Abundance..130C":15944.86095,"Abundance..131":14962.26884,"Ions.Score":19,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":0.07469,"Percolator.q.Value":0,"Percolator.PEP":0.00009}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (M2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].lFVGNLPPDITEEEMr.[K]","Modifications":"N-Term(TMT6plex); R16(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q15233","Master.Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","Protein.Accessions":"Q15233","Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":1050.05054,"MH...Da.":2099.0938,"Theo..MH...Da.":2099.09198,"DeltaM..ppm.":0.87,"Deltam.z..Da.":0.00091,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":30.96852,"Average.Reporter.S.N":12.1,"Ion.Inject.Time..ms.":50,"RT..min.":176.958,"First.Scan":89883,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_03.raw","File.ID":"F3","Abundance..126":6380.88257,"Abundance..127N":5536.06081,"Abundance..127C":6453.6703,"Abundance..128N":7762.63643,"Abundance..128C":6148.10087,"Abundance..129N":5814.69839,"Abundance..129C":7394.6653,"Abundance..130N":6194.58959,"Abundance..130C":4527.6172,"Abundance..131":5120.62823,"Ions.Score":33,"Identity.Strict":27,"Identity.Relaxed":20,"Expectation.Value":0.003,"Percolator.q.Value":0,"Percolator.PEP":0.00002}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (L2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].lGFSEVELVQMVVDGVk.[L]","Modifications":"N-Term(TMT6plex); K17(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P12277","Master.Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","Protein.Accessions":"P12277","Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.8,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":772.44446,"MH...Da.":2315.31882,"Theo..MH...Da.":2315.31763,"DeltaM..ppm.":0.52,"Deltam.z..Da.":0.0004,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":4.51349,"Average.Reporter.S.N":20.7,"Ion.Inject.Time..ms.":39.315,"RT..min.":222.8534,"First.Scan":113830,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_02.raw","File.ID":"F4","Abundance..126":7795.95739,"Abundance..127N":5784.43779,"Abundance..127C":7720.08168,"Abundance..128N":6579.92862,"Abundance..128C":7392.45068,"Abundance..129N":6732.75792,"Abundance..129C":9087.07322,"Abundance..130N":6136.04909,"Abundance..130C":6916.83153,"Abundance..131":5982.89014,"Ions.Score":30,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.00994,"Percolator.q.Value":0,"Percolator.PEP":1.162e-07}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (M2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].sLQDIIAILGmDELSEEDk.[L]","Modifications":"N-Term(TMT6plex); M11(Oxidation); K19(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":867.79987,"MH...Da.":2601.38504,"Theo..MH...Da.":2601.38247,"DeltaM..ppm.":0.99,"Deltam.z..Da.":0.00086,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":10.96401,"Average.Reporter.S.N":26.3,"Ion.Inject.Time..ms.":38.9,"RT..min.":221.5527,"First.Scan":112845,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_03.raw","File.ID":"F3","Abundance..126":9113.72965,"Abundance..127N":7515.42597,"Abundance..127C":9658.45727,"Abundance..128N":8500.51342,"Abundance..128C":9303.14919,"Abundance..129N":9210.73264,"Abundance..129C":9935.92254,"Abundance..130N":9613.17031,"Abundance..130C":8404.80379,"Abundance..131":11289.35433,"Ions.Score":52,"Identity.Strict":30,"Identity.Relaxed":23,"Expectation.Value":0.00007,"Percolator.q.Value":0,"Percolator.PEP":3.813e-08}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (L2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].eVLGDAVPDEILIEAVLk.[N]","Modifications":"N-Term(TMT6plex); K18(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q9Y450","Master.Protein.Descriptions":"HBS1-like protein OS=Homo sapiens GN=HBS1L PE=1 SV=1","Protein.Accessions":"Q9Y450","Protein.Descriptions":"HBS1-like protein OS=Homo sapiens GN=HBS1L PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.8846,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":797.14233,"MH...Da.":2389.41245,"Theo..MH...Da.":2389.40855,"DeltaM..ppm.":1.63,"Deltam.z..Da.":0.0013,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":6.4025,"Average.Reporter.S.N":18.8,"Ion.Inject.Time..ms.":46.477,"RT..min.":218.2809,"First.Scan":111730,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_02.raw","File.ID":"F4","Abundance..126":6896.67064,"Abundance..127N":7178.17293,"Abundance..127C":5629.40685,"Abundance..128N":6606.00339,"Abundance..128C":8047.38857,"Abundance..129N":6784.07335,"Abundance..129C":5065.8496,"Abundance..130N":5648.2882,"Abundance..130C":6115.30322,"Abundance..131":7337.94431,"Ions.Score":26,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.01978,"Percolator.q.Value":0,"Percolator.PEP":0.00003}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (D2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].vVDLMAHmASkE.[-]","Modifications":"N-Term(TMT6plex); M8(Oxidation); K11(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":1,"Charge":3,"DeltaScore":0.7778,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":604.99976,"MH...Da.":1812.98471,"Theo..MH...Da.":1812.98435,"DeltaM..ppm.":0.2,"Deltam.z..Da.":0.00012,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":24.37405,"Average.Reporter.S.N":28.9,"Ion.Inject.Time..ms.":6.614,"RT..min.":114.6103,"First.Scan":56661,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_02.raw","File.ID":"F12","Abundance..126":121840.60282,"Abundance..127N":112864.51476,"Abundance..127C":138745.76523,"Abundance..128N":124319.18703,"Abundance..128C":125733.04762,"Abundance..129N":148284.44757,"Abundance..129C":122964.23059,"Abundance..130N":143162.84819,"Abundance..130C":130505.75906,"Abundance..131":149649.7567,"Ions.Score":18,"Identity.Strict":30,"Identity.Relaxed":23,"Expectation.Value":0.16999,"Percolator.q.Value":0,"Percolator.PEP":0.00006}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (D2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].lAVEALSSLDGDLAGr.[Y]","Modifications":"N-Term(TMT6plex); R16(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P12277","Master.Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","Protein.Accessions":"P12277","Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.9661,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":609.34174,"MH...Da.":1826.01065,"Theo..MH...Da.":1826.00963,"DeltaM..ppm.":0.56,"Deltam.z..Da.":0.00034,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":3.62554,"Average.Reporter.S.N":36.8,"Ion.Inject.Time..ms.":3.134,"RT..min.":181.9094,"First.Scan":96221,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_02.raw","File.ID":"F12","Abundance..126":152896.43113,"Abundance..127N":226447.63326,"Abundance..127C":203554.88764,"Abundance..128N":209990.10137,"Abundance..128C":219164.14386,"Abundance..129N":215358.86034,"Abundance..129C":206019.41298,"Abundance..130N":233031.29768,"Abundance..130C":173970.80654,"Abundance..131":236646.58783,"Ions.Score":59,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.00001,"Percolator.q.Value":0,"Percolator.PEP":1.04e-08}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (D2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].mGQMAMGGAMGINNr.[G]","Modifications":"N-Term(TMT6plex); R15(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q15233","Master.Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","Protein.Accessions":"Q15233","Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":889.42517,"MH...Da.":1777.84307,"Theo..MH...Da.":1777.84067,"DeltaM..ppm.":1.35,"Deltam.z..Da.":0.0012,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":0,"Average.Reporter.S.N":6,"Ion.Inject.Time..ms.":24.535,"RT..min.":127.1819,"First.Scan":64067,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_02.raw","File.ID":"F12","Abundance..127N":5162.63676,"Abundance..127C":5364.58316,"Abundance..128N":4977.08922,"Abundance..128C":4369.40266,"Abundance..129N":5368.74642,"Abundance..129C":2859.13304,"Abundance..130N":5871.91673,"Abundance..130C":4036.38071,"Abundance..131":3720.46587,"Ions.Score":17,"Identity.Strict":21,"Identity.Relaxed":14,"Expectation.Value":0.02926,"Percolator.q.Value":0,"Percolator.PEP":8.407e-07}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (D2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].vPTANVSVVDLTcr.[L]","Modifications":"N-Term(TMT6plex); C13(Carbamidomethyl); R14(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":0.9077,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":885.48706,"MH...Da.":1769.96684,"Theo..MH...Da.":1769.96565,"DeltaM..ppm.":0.67,"Deltam.z..Da.":0.0006,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":25.82087,"Average.Reporter.S.N":6.2,"Ion.Inject.Time..ms.":1.874,"RT..min.":147.0955,"First.Scan":75802,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_02.raw","File.ID":"F12","Abundance..126":101985.77289,"Abundance..127N":70916.37411,"Abundance..127C":91872.70035,"Abundance..128N":192472.79777,"Abundance..128C":151214.72334,"Abundance..129N":72106.0971,"Abundance..129C":109592.29845,"Abundance..130N":128861.5898,"Abundance..130C":112353.7759,"Abundance..131":96954.01605,"Ions.Score":65,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":2.98826e-06,"Percolator.q.Value":0,"Percolator.PEP":6.422e-08}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (L2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].dYEFMWNPHLGYILTcPSNLGTGLr.[A]","Modifications":"N-Term(TMT6plex); C16(Carbamidomethyl); R25(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P12277","Master.Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","Protein.Accessions":"P12277","Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","X..Missed.Cleavages":0,"Charge":4,"DeltaScore":0.7879,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":799.14844,"MH...Da.":3193.57192,"Theo..MH...Da.":3193.5676,"DeltaM..ppm.":1.35,"Deltam.z..Da.":0.00108,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":14.59466,"Average.Reporter.S.N":26.4,"Ion.Inject.Time..ms.":22.507,"RT..min.":190.2547,"First.Scan":97667,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_02.raw","File.ID":"F4","Abundance..126":75081.84969,"Abundance..127N":84940.3276,"Abundance..127C":92749.98563,"Abundance..128N":87161.51201,"Abundance..128C":94158.91418,"Abundance..129N":92527.92233,"Abundance..129C":95908.39874,"Abundance..130N":92905.33633,"Abundance..130C":95538.70386,"Abundance..131":66697.73753,"Ions.Score":33,"Identity.Strict":26,"Identity.Relaxed":19,"Expectation.Value":0.00216,"Percolator.q.Value":0,"Percolator.PEP":9.244e-06}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (M2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].vDIVAINDPFIDLNYMVYMFQYDSTHGk.[F]","Modifications":"N-Term(TMT6plex); K28(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":0,"Charge":4,"DeltaScore":0.9697,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":944.48102,"MH...Da.":3774.90224,"Theo..MH...Da.":3774.90431,"DeltaM..ppm.":-0.55,"Deltam.z..Da.":-0.00052,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":100,"Average.Reporter.S.N":11.1,"Ion.Inject.Time..ms.":50,"RT..min.":240.0568,"First.Scan":121106,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_03.raw","File.ID":"F3","Abundance..126":2849.29947,"Abundance..127N":3619.71319,"Abundance..127C":2717.06287,"Abundance..128N":4189.57062,"Abundance..128C":3674.51629,"Abundance..129N":3940.49712,"Abundance..129C":3487.6647,"Abundance..130N":2388.55037,"Abundance..130C":5239.16333,"Abundance..131":4608.40861,"Ions.Score":33,"Identity.Strict":27,"Identity.Relaxed":20,"Expectation.Value":0.00302,"Percolator.q.Value":0,"Percolator.PEP":2.082e-06}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (D2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].tFLVWVNEEDHLr.[V]","Modifications":"N-Term(TMT6plex); R13(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P12277","Master.Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","Protein.Accessions":"P12277","Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":633.00665,"MH...Da.":1897.00541,"Theo..MH...Da.":1897.00448,"DeltaM..ppm.":0.49,"Deltam.z..Da.":0.00031,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":22.43647,"Average.Reporter.S.N":32.3,"Ion.Inject.Time..ms.":0.901,"RT..min.":160.533,"First.Scan":83749,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_02.raw","File.ID":"F12","Abundance..126":533530.59375,"Abundance..127N":553210.67934,"Abundance..127C":481953.62807,"Abundance..128N":660365.50658,"Abundance..128C":584264.35286,"Abundance..129N":661094.50542,"Abundance..129C":616986.10108,"Abundance..130N":538325.18423,"Abundance..130C":684499.05318,"Abundance..131":600309.06342,"Ions.Score":45,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.00027,"Percolator.q.Value":0,"Percolator.PEP":6.078e-07}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (D2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[K].vALVYGQMNEPPGAr.[A]","Modifications":"N-Term(TMT6plex); R15(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.9608,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":614.33221,"MH...Da.":1840.98209,"Theo..MH...Da.":1840.98164,"DeltaM..ppm.":0.25,"Deltam.z..Da.":0.00015,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":27.67483,"Average.Reporter.S.N":32.9,"Ion.Inject.Time..ms.":2.231,"RT..min.":136.6784,"First.Scan":69648,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_02.raw","File.ID":"F12","Abundance..126":185701.53588,"Abundance..127N":197232.79623,"Abundance..127C":221055.12269,"Abundance..128N":263487.34001,"Abundance..128C":164693.24212,"Abundance..129N":206349.47955,"Abundance..129C":194035.07716,"Abundance..130N":185190.75869,"Abundance..130C":186587.24848,"Abundance..131":182964.05478,"Ions.Score":51,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.00007,"Percolator.q.Value":0,"Percolator.PEP":1.968e-06}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (D2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[K].lAAVTYNGVDNNk.[N]","Modifications":"N-Term(TMT6plex); K13(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q16181","Master.Protein.Descriptions":"Septin-7 OS=Homo sapiens GN=SEPT7 PE=1 SV=2","Protein.Accessions":"Q16181","Protein.Descriptions":"Septin-7 OS=Homo sapiens GN=SEPT7 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":0.8804,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":923.0235,"MH...Da.":1845.03972,"Theo..MH...Da.":1845.03618,"DeltaM..ppm.":1.92,"Deltam.z..Da.":0.00177,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":0,"Average.Reporter.S.N":22.4,"Ion.Inject.Time..ms.":19.304,"RT..min.":120.6074,"First.Scan":60190,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_02.raw","File.ID":"F12","Abundance..126":7144.64479,"Abundance..127N":6801.36075,"Abundance..127C":6833.89705,"Abundance..128N":9169.93793,"Abundance..128C":9187.32979,"Abundance..129N":5381.0151,"Abundance..129C":7685.70804,"Abundance..130N":6890.41573,"Abundance..130C":8467.40194,"Abundance..131":7678.61457,"Ions.Score":92,"Identity.Strict":30,"Identity.Relaxed":23,"Expectation.Value":7.04502e-09,"Percolator.q.Value":0,"Percolator.PEP":2.852e-06}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (L2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].sTPSGFTLDDVIQTGVDNPGHPYIMTVGcVAGDEESYEVFk.[D]","Modifications":"N-Term(TMT6plex); C29(Carbamidomethyl); K41(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P12277","Master.Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","Protein.Accessions":"P12277","Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","X..Missed.Cleavages":0,"Charge":4,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":1225.349,"MH...Da.":4898.37417,"Theo..MH...Da.":4898.36724,"DeltaM..ppm.":1.41,"Deltam.z..Da.":0.00173,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":0,"Average.Reporter.S.N":13.8,"Ion.Inject.Time..ms.":48.554,"RT..min.":198.0933,"First.Scan":101840,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_02.raw","File.ID":"F4","Abundance..126":5025.85801,"Abundance..127N":4638.78735,"Abundance..127C":4210.39623,"Abundance..128N":3477.20077,"Abundance..128C":4291.52727,"Abundance..129N":5651.3277,"Abundance..129C":5691.73487,"Abundance..130N":5456.38042,"Abundance..130C":3170.54188,"Abundance..131":5117.79561,"Ions.Score":67,"Identity.Strict":22,"Identity.Relaxed":15,"Expectation.Value":4.19521e-07,"Percolator.q.Value":0,"Percolator.PEP":8.118e-12}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (D2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[R].sAYQEAMDISk.[K]","Modifications":"N-Term(TMT6plex); K11(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P31947","Master.Protein.Descriptions":"14-3-3 protein sigma OS=Homo sapiens GN=SFN PE=1 SV=1","Protein.Accessions":"P31947","Protein.Descriptions":"14-3-3 protein sigma OS=Homo sapiens GN=SFN PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":0.9403,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":854.9585,"MH...Da.":1708.90972,"Theo..MH...Da.":1708.90714,"DeltaM..ppm.":1.51,"Deltam.z..Da.":0.00129,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":31.33638,"Average.Reporter.S.N":21.4,"Ion.Inject.Time..ms.":50,"RT..min.":123.5984,"First.Scan":61956,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_02.raw","File.ID":"F12","Abundance..126":11881.7529,"Abundance..127N":13755.12919,"Abundance..127C":14772.56342,"Abundance..128N":11044.23218,"Abundance..128C":10217.88004,"Abundance..129N":14365.80398,"Abundance..129C":12096.19913,"Abundance..130N":10808.34059,"Abundance..130C":12480.24604,"Abundance..131":10357.19553,"Ions.Score":67,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":1.34616e-06,"Percolator.q.Value":0,"Percolator.PEP":2.605e-07}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (L2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[K].gSITSVQAIYVPADDLTDPAPATTFAHLDATTVLSr.[A]","Modifications":"N-Term(TMT6plex); R36(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":4,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":989.27075,"MH...Da.":3954.06118,"Theo..MH...Da.":3954.05731,"DeltaM..ppm.":0.98,"Deltam.z..Da.":0.00097,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":1.17534,"Average.Reporter.S.N":8.1,"Ion.Inject.Time..ms.":2,"RT..min.":190.2988,"First.Scan":97690,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_02.raw","File.ID":"F4","Abundance..126":22105.83213,"Abundance..127N":41419.279,"Abundance..127C":46792.24003,"Abundance..128N":50619.90696,"Abundance..128C":56013.08334,"Abundance..129N":38743.72681,"Abundance..129C":37516.16764,"Abundance..130N":30920.55939,"Abundance..130C":44933.52313,"Abundance..131":47821.55043,"Ions.Score":38,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":0.00129,"Percolator.q.Value":0,"Percolator.PEP":1.158e-07}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (L2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].cSEGSFLLTTFPrPVTVEPMDQLDDEEGLPEk.[L]","Modifications":"N-Term(TMT6plex); C1(Carbamidomethyl); R13(Label:13C(6)15N(4)); K32(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q15233","Master.Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","Protein.Accessions":"Q15233","Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","X..Missed.Cleavages":1,"Charge":4,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":1029.02087,"MH...Da.":4113.06167,"Theo..MH...Da.":4113.05674,"DeltaM..ppm.":1.2,"Deltam.z..Da.":0.00123,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":7.3134,"Average.Reporter.S.N":57.2,"Ion.Inject.Time..ms.":12.599,"RT..min.":187.6997,"First.Scan":96305,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_02.raw","File.ID":"F4","Abundance..126":154599.86654,"Abundance..127N":162775.82027,"Abundance..127C":160191.4106,"Abundance..128N":125780.1692,"Abundance..128C":149279.90161,"Abundance..129N":164695.2407,"Abundance..129C":159158.92428,"Abundance..130N":131614.03269,"Abundance..130C":175815.2794,"Abundance..131":166468.99669,"Ions.Score":77,"Identity.Strict":26,"Identity.Relaxed":19,"Expectation.Value":8.92774e-08,"Percolator.q.Value":0,"Percolator.PEP":3.438e-08}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (C2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].iGLFGGAGVGk.[T]","Modifications":"N-Term(TMT6plex); K11(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":0.8983,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":721.45526,"MH...Da.":1441.90325,"Theo..MH...Da.":1441.90226,"DeltaM..ppm.":0.69,"Deltam.z..Da.":0.0005,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":32.59981,"Average.Reporter.S.N":39.4,"Ion.Inject.Time..ms.":2.252,"RT..min.":153.1661,"First.Scan":78231,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture4_01.raw","File.ID":"F13","Abundance..126":306606.13354,"Abundance..127N":354837.83624,"Abundance..127C":317504.43065,"Abundance..128N":302377.74753,"Abundance..128C":307199.2156,"Abundance..129N":314239.19713,"Abundance..129C":307217.04125,"Abundance..130N":290990.26616,"Abundance..130C":297190.55745,"Abundance..131":302738.59137,"Ions.Score":59,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":9.59972e-06,"Percolator.q.Value":0,"Percolator.PEP":0.00006}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (D2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].eNFSLDWck.[Q]","Modifications":"N-Term(TMT6plex); C8(Carbamidomethyl); K9(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P23919","Master.Protein.Descriptions":"Thymidylate kinase OS=Homo sapiens GN=DTYMK PE=1 SV=4","Protein.Accessions":"P23919","Protein.Descriptions":"Thymidylate kinase OS=Homo sapiens GN=DTYMK PE=1 SV=4","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":0.9412,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":832.93341,"MH...Da.":1664.85954,"Theo..MH...Da.":1664.8598,"DeltaM..ppm.":-0.15,"Deltam.z..Da.":-0.00013,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":11.71194,"Average.Reporter.S.N":25.8,"Ion.Inject.Time..ms.":4.877,"RT..min.":155.6173,"First.Scan":80839,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_02.raw","File.ID":"F12","Abundance..126":64142.88205,"Abundance..127N":60682.12926,"Abundance..127C":52104.08361,"Abundance..128N":69940.17081,"Abundance..128C":70072.3746,"Abundance..129N":53709.40932,"Abundance..129C":43104.83371,"Abundance..130N":66747.31227,"Abundance..130C":56933.96685,"Abundance..131":75907.25328,"Ions.Score":51,"Identity.Strict":27,"Identity.Relaxed":20,"Expectation.Value":0.00004,"Percolator.q.Value":0,"Percolator.PEP":0.00003}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (D2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].fTQAGSEVSALLGr.[I]","Modifications":"N-Term(TMT6plex); R14(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":837.96759,"MH...Da.":1674.9279,"Theo..MH...Da.":1674.92517,"DeltaM..ppm.":1.63,"Deltam.z..Da.":0.00137,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":9.88874,"Average.Reporter.S.N":10.9,"Ion.Inject.Time..ms.":1.027,"RT..min.":162.6827,"First.Scan":85018,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_02.raw","File.ID":"F12","Abundance..126":161448.38388,"Abundance..127N":166715.31324,"Abundance..127C":208921.46774,"Abundance..128N":276397.72629,"Abundance..128C":177875.15116,"Abundance..129N":166696.27028,"Abundance..129C":243453.98349,"Abundance..130N":229456.24332,"Abundance..130C":233248.0362,"Abundance..131":234676.48915,"Ions.Score":81,"Identity.Strict":30,"Identity.Relaxed":23,"Expectation.Value":7.63731e-08,"Percolator.q.Value":0,"Percolator.PEP":3.566e-08}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (D2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[R].vALTGLTVAEYFr.[D]","Modifications":"N-Term(TMT6plex); R13(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.9815,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":560.32538,"MH...Da.":1678.96158,"Theo..MH...Da.":1678.96049,"DeltaM..ppm.":0.65,"Deltam.z..Da.":0.00036,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":8.30122,"Average.Reporter.S.N":63.3,"Ion.Inject.Time..ms.":9.156,"RT..min.":182.103,"First.Scan":96330,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_02.raw","File.ID":"F12","Abundance..126":102959.74153,"Abundance..127N":106229.41047,"Abundance..127C":99710.48911,"Abundance..128N":105195.72653,"Abundance..128C":94485.9199,"Abundance..129N":104600.22096,"Abundance..129C":97486.8664,"Abundance..130N":102533.46452,"Abundance..130C":99522.34412,"Abundance..131":91196.88434,"Ions.Score":54,"Identity.Strict":30,"Identity.Relaxed":23,"Expectation.Value":0.00004,"Percolator.q.Value":0,"Percolator.PEP":2.545e-06}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (L2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[K].qFAPIHAEAPEFMEMSVEQEILVTGIk.[V]","Modifications":"N-Term(TMT6plex); K27(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":4,"DeltaScore":0.8333,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":878.46771,"MH...Da.":3510.84902,"Theo..MH...Da.":3510.85082,"DeltaM..ppm.":-0.51,"Deltam.z..Da.":-0.00045,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":4.99452,"Average.Reporter.S.N":9.3,"Ion.Inject.Time..ms.":1.465,"RT..min.":196.4293,"First.Scan":100950,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_02.raw","File.ID":"F4","Abundance..126":49014.44408,"Abundance..127N":56170.92703,"Abundance..127C":58139.09112,"Abundance..128N":52347.80662,"Abundance..128C":57105.62462,"Abundance..129N":59019.11556,"Abundance..129C":52935.65015,"Abundance..130N":68960.75977,"Abundance..130C":44476.38944,"Abundance..131":49901.81723,"Ions.Score":12,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.57805,"Percolator.q.Value":0,"Percolator.PEP":0.00005}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (D2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].nPGFEIIHGLLDr.[I]","Modifications":"N-Term(TMT6plex); R13(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q9NSD9","Master.Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","Protein.Accessions":"Q9NSD9","Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":573.99249,"MH...Da.":1719.96292,"Theo..MH...Da.":1719.96189,"DeltaM..ppm.":0.6,"Deltam.z..Da.":0.00035,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":25.35543,"Average.Reporter.S.N":48.2,"Ion.Inject.Time..ms.":5.692,"RT..min.":165.9447,"First.Scan":86947,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_02.raw","File.ID":"F12","Abundance..126":251322.39997,"Abundance..127N":222510.42752,"Abundance..127C":226627.19321,"Abundance..128N":230776.83834,"Abundance..128C":285476.35161,"Abundance..129N":243146.9854,"Abundance..129C":264154.80846,"Abundance..130N":242247.05613,"Abundance..130C":238123.43598,"Abundance..131":220620.33892,"Ions.Score":43,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.00045,"Percolator.q.Value":0,"Percolator.PEP":6.254e-06}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (C2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].iMNVIGEPIDEr.[G]","Modifications":"N-Term(TMT6plex); R12(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":0.9451,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":812.94446,"MH...Da.":1624.88164,"Theo..MH...Da.":1624.88053,"DeltaM..ppm.":0.68,"Deltam.z..Da.":0.00056,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":16.96054,"Average.Reporter.S.N":38.2,"Ion.Inject.Time..ms.":7.216,"RT..min.":155.2992,"First.Scan":79465,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture4_01.raw","File.ID":"F13","Abundance..126":126541.27207,"Abundance..127N":110384.87358,"Abundance..127C":94663.40106,"Abundance..128N":103113.29537,"Abundance..128C":99028.70805,"Abundance..129N":131244.60092,"Abundance..129C":113318.16052,"Abundance..130N":117319.27346,"Abundance..130C":112087.97787,"Abundance..131":91444.65295,"Ions.Score":91,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":5.52461e-09,"Percolator.q.Value":0,"Percolator.PEP":0.00001}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (L4)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].vPTANVSVVDLTcR.[L]","Modifications":"N-Term(TMT6plex); C13(Carbamidomethyl)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":587.32532,"MH...Da.":1759.9614,"Theo..MH...Da.":1759.95739,"DeltaM..ppm.":2.28,"Deltam.z..Da.":0.00134,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":6.1247,"Average.Reporter.S.N":54.6,"Ion.Inject.Time..ms.":6.425,"RT..min.":146.9539,"First.Scan":73283,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_02.raw","File.ID":"F4","Abundance..126":102420.46187,"Abundance..127N":78261.08591,"Abundance..127C":98746.64301,"Abundance..128N":99610.84053,"Abundance..128C":99543.4447,"Abundance..129N":107678.67137,"Abundance..129C":109405.89667,"Abundance..130N":92041.18414,"Abundance..130C":87722.95016,"Abundance..131":108890.32075,"Ions.Score":43,"Identity.Strict":30,"Identity.Relaxed":23,"Expectation.Value":0.00054,"Percolator.q.Value":0,"Percolator.PEP":0.00773}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (L4)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].iISNAScTTNcLAPLAk.[V]","Modifications":"N-Term(TMT6plex); C7(Carbamidomethyl); C11(Carbamidomethyl); K17(TMT6plex)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.86,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":764.75391,"MH...Da.":2292.24717,"Theo..MH...Da.":2292.24559,"DeltaM..ppm.":0.69,"Deltam.z..Da.":0.00052,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":21.4758,"Average.Reporter.S.N":37.1,"Ion.Inject.Time..ms.":3.745,"RT..min.":149.6988,"First.Scan":74841,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_02.raw","File.ID":"F4","Abundance..126":120890.02558,"Abundance..127N":123305.92656,"Abundance..127C":131093.84611,"Abundance..128N":147504.86523,"Abundance..128C":119630.36401,"Abundance..129N":138691.00927,"Abundance..129C":139902.71256,"Abundance..130N":109131.37533,"Abundance..130C":128129.14999,"Abundance..131":115224.98348,"Ions.Score":50,"Identity.Strict":30,"Identity.Relaxed":23,"Expectation.Value":0.00012,"Percolator.q.Value":0,"Percolator.PEP":0.00243}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (M2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].dYEFMWNPHLGYILTcPSNLGTGLr.[A]","Modifications":"N-Term(TMT6plex); C16(Carbamidomethyl); R25(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P12277","Master.Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","Protein.Accessions":"P12277","Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":1065.19446,"MH...Da.":3193.56882,"Theo..MH...Da.":3193.5676,"DeltaM..ppm.":0.38,"Deltam.z..Da.":0.00041,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":7.93348,"Average.Reporter.S.N":17.5,"Ion.Inject.Time..ms.":10.105,"RT..min.":190.3162,"First.Scan":97280,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_03.raw","File.ID":"F3","Abundance..126":25816.59773,"Abundance..127N":27606.58143,"Abundance..127C":28319.12395,"Abundance..128N":26756.50909,"Abundance..128C":33143.93828,"Abundance..129N":24799.4397,"Abundance..129C":29617.7416,"Abundance..130N":32408.93593,"Abundance..130C":30239.17797,"Abundance..131":31110.82563,"Ions.Score":60,"Identity.Strict":26,"Identity.Relaxed":19,"Expectation.Value":4.52327e-06,"Percolator.q.Value":0,"Percolator.PEP":2.012e-06}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (L2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].aDIIHAcDIVEDAAIAYGYNNIQMTLPk.[T]","Modifications":"N-Term(TMT6plex); C7(Carbamidomethyl); K28(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q9NSD9","Master.Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","Protein.Accessions":"Q9NSD9","Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","X..Missed.Cleavages":0,"Charge":4,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":897.21985,"MH...Da.":3585.85756,"Theo..MH...Da.":3585.8577,"DeltaM..ppm.":-0.04,"Deltam.z..Da.":-0.00003,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":14.74043,"Average.Reporter.S.N":13.2,"Ion.Inject.Time..ms.":39.583,"RT..min.":205.0111,"First.Scan":105368,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_02.raw","File.ID":"F4","Abundance..126":6386.43721,"Abundance..127N":6760.70032,"Abundance..127C":7315.2996,"Abundance..128N":5558.13578,"Abundance..128C":6162.70881,"Abundance..129N":9180.75462,"Abundance..129C":4962.6909,"Abundance..130N":4860.18608,"Abundance..130C":6184.1518,"Abundance..131":4537.2569,"Ions.Score":19,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":0.08832,"Percolator.q.Value":0,"Percolator.PEP":6.592e-07}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (M2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].sTLINSLFLTDLYSPEYPGPSHr.[I]","Modifications":"N-Term(TMT6plex); R23(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q16181","Master.Protein.Descriptions":"Septin-7 OS=Homo sapiens GN=SEPT7 PE=1 SV=2","Protein.Accessions":"Q16181","Protein.Descriptions":"Septin-7 OS=Homo sapiens GN=SEPT7 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":949.50073,"MH...Da.":2846.48764,"Theo..MH...Da.":2846.48014,"DeltaM..ppm.":2.63,"Deltam.z..Da.":0.0025,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":14.9591,"Average.Reporter.S.N":4.6,"Ion.Inject.Time..ms.":10.551,"RT..min.":187.917,"First.Scan":95994,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_03.raw","File.ID":"F3","Abundance..126":5184.52564,"Abundance..127N":2531.22699,"Abundance..127C":5277.29134,"Abundance..128N":6094.57877,"Abundance..128C":8118.7022,"Abundance..129N":2813.07803,"Abundance..129C":6112.12553,"Abundance..130N":6485.88108,"Abundance..130C":2808.19059,"Abundance..131":4249.74814,"Ions.Score":62,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":5.99802e-06,"Percolator.q.Value":0,"Percolator.PEP":9.223e-07}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (M2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].dLFDPIIEDr.[H]","Modifications":"N-Term(TMT6plex); R10(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P12277","Master.Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","Protein.Accessions":"P12277","Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":0.8696,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":736.39813,"MH...Da.":1471.78899,"Theo..MH...Da.":1471.78694,"DeltaM..ppm.":1.39,"Deltam.z..Da.":0.00102,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":2.82004,"Average.Reporter.S.N":7.1,"Ion.Inject.Time..ms.":5.761,"RT..min.":168.9785,"First.Scan":85363,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_03.raw","File.ID":"F3","Abundance..126":65736.06454,"Abundance..127N":56414.60571,"Abundance..127C":68297.0598,"Abundance..128N":47703.09357,"Abundance..128C":96920.86077,"Abundance..129N":85227.27307,"Abundance..129C":67519.05442,"Abundance..130N":74505.69316,"Abundance..130C":70792.7438,"Abundance..131":97953.97109,"Ions.Score":69,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":1.00074e-06,"Percolator.q.Value":0,"Percolator.PEP":0.00009}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (M2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].gEELScEEr.[N]","Modifications":"N-Term(TMT6plex); C6(Carbamidomethyl); R9(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P31947","Master.Protein.Descriptions":"14-3-3 protein sigma OS=Homo sapiens GN=SFN PE=1 SV=1","Protein.Accessions":"P31947","Protein.Descriptions":"14-3-3 protein sigma OS=Homo sapiens GN=SFN PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":674.31873,"MH...Da.":1347.63017,"Theo..MH...Da.":1347.62873,"DeltaM..ppm.":1.07,"Deltam.z..Da.":0.00072,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":0,"Average.Reporter.S.N":36.2,"Ion.Inject.Time..ms.":48.004,"RT..min.":47.8529,"First.Scan":18723,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_03.raw","File.ID":"F3","Abundance..126":15719.08623,"Abundance..127N":14476.30074,"Abundance..127C":14311.68526,"Abundance..128N":18389.54914,"Abundance..128C":16957.8346,"Abundance..129N":12845.38599,"Abundance..129C":17901.20104,"Abundance..130N":18422.07685,"Abundance..130C":14236.49864,"Abundance..131":15068.29944,"Ions.Score":56,"Identity.Strict":19,"Identity.Relaxed":13,"Expectation.Value":1.87971e-06,"Percolator.q.Value":0,"Percolator.PEP":0.00011}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (M2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].tVLIMELINNVAk.[A]","Modifications":"N-Term(TMT6plex); K13(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":962.59399,"MH...Da.":1924.18071,"Theo..MH...Da.":1924.17968,"DeltaM..ppm.":0.54,"Deltam.z..Da.":0.00052,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":16.6431,"Average.Reporter.S.N":40.3,"Ion.Inject.Time..ms.":32.704,"RT..min.":208.3606,"First.Scan":106634,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_03.raw","File.ID":"F3","Abundance..126":12486.70061,"Abundance..127N":13158.08678,"Abundance..127C":13086.97771,"Abundance..128N":14080.25881,"Abundance..128C":15022.52968,"Abundance..129N":11815.85399,"Abundance..129C":15065.3195,"Abundance..130N":12607.69077,"Abundance..130C":12519.85312,"Abundance..131":13864.51301,"Ions.Score":37,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":0.00136,"Percolator.q.Value":0,"Percolator.PEP":1.082e-06}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (C2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[R].aAPGAEFAPNk.[R]","Modifications":"N-Term(TMT6plex); K11(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q15233","Master.Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","Protein.Accessions":"Q15233","Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":0.9815,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":769.94611,"MH...Da.":1538.88494,"Theo..MH...Da.":1538.88225,"DeltaM..ppm.":1.75,"Deltam.z..Da.":0.00134,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":9.58569,"Average.Reporter.S.N":34.8,"Ion.Inject.Time..ms.":4.73,"RT..min.":100.6477,"First.Scan":48601,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture4_01.raw","File.ID":"F13","Abundance..126":174009.3979,"Abundance..127N":201732.42983,"Abundance..127C":147609.69552,"Abundance..128N":171247.8851,"Abundance..128C":185073.781,"Abundance..129N":151511.10707,"Abundance..129C":172618.67385,"Abundance..130N":181776.73316,"Abundance..130C":163711.96039,"Abundance..131":146716.3858,"Ions.Score":54,"Identity.Strict":30,"Identity.Relaxed":23,"Expectation.Value":0.00004,"Percolator.q.Value":0,"Percolator.PEP":0.00005}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (L2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].wGDAGAEYVVESTGVFTTMEk.[A]","Modifications":"N-Term(TMT6plex); K21(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":915.13342,"MH...Da.":2743.38572,"Theo..MH...Da.":2743.37805,"DeltaM..ppm.":2.79,"Deltam.z..Da.":0.00255,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":7.93394,"Average.Reporter.S.N":13.3,"Ion.Inject.Time..ms.":30.422,"RT..min.":214.2865,"First.Scan":109867,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_02.raw","File.ID":"F4","Abundance..126":4792.01321,"Abundance..127N":3882.807,"Abundance..127C":3584.26478,"Abundance..128N":4139.48817,"Abundance..128C":5043.26372,"Abundance..129N":4707.61354,"Abundance..129C":4627.10825,"Abundance..130N":4454.63153,"Abundance..130C":4839.24894,"Abundance..131":5086.67691,"Ions.Score":29,"Identity.Strict":27,"Identity.Relaxed":20,"Expectation.Value":0.00653,"Percolator.q.Value":0,"Percolator.PEP":0.00001}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (M2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[R].dQEGQDVLLFIDNIFr.[F]","Modifications":"N-Term(TMT6plex); R16(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":0.9908,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":1081.073,"MH...Da.":2161.13872,"Theo..MH...Da.":2161.13662,"DeltaM..ppm.":0.97,"Deltam.z..Da.":0.00105,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":22.17281,"Average.Reporter.S.N":10.3,"Ion.Inject.Time..ms.":50,"RT..min.":231.6806,"First.Scan":117408,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_03.raw","File.ID":"F3","Abundance..126":3408.32135,"Abundance..127N":3564.20186,"Abundance..127C":2920.98246,"Abundance..128N":3238.01673,"Abundance..128C":3635.65529,"Abundance..129N":3085.14642,"Abundance..129C":3822.0312,"Abundance..130N":3661.48032,"Abundance..130C":3935.76552,"Abundance..131":3602.28271,"Ions.Score":109,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":8.15279e-11,"Percolator.q.Value":0,"Percolator.PEP":5.368e-09}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (M2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].sMTEAEQQQLIDDHFLFDkPVSPLLLASGMAr.[D]","Modifications":"N-Term(TMT6plex); K19(Label); R32(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P12277","Master.Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","Protein.Accessions":"P12277","Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","X..Missed.Cleavages":1,"Charge":4,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":1016.79138,"MH...Da.":4064.1437,"Theo..MH...Da.":4064.1356,"DeltaM..ppm.":1.99,"Deltam.z..Da.":0.00203,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":35.95441,"Average.Reporter.S.N":12.8,"Ion.Inject.Time..ms.":10.064,"RT..min.":189.8159,"First.Scan":97004,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_03.raw","File.ID":"F3","Abundance..126":12330.44154,"Abundance..127N":17196.12559,"Abundance..127C":16950.78959,"Abundance..128N":9602.26781,"Abundance..128C":16656.19751,"Abundance..129N":14901.73269,"Abundance..129C":14615.87386,"Abundance..130N":19158.8492,"Abundance..130C":16730.96165,"Abundance..131":20185.43722,"Ions.Score":42,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":0.00039,"Percolator.q.Value":0,"Percolator.PEP":0.00002}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (M2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].gSITSVQAIYVPADDLTDPAPATTFAHLDATTVLSr.[A]","Modifications":"N-Term(TMT6plex); R36(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":1318.69055,"MH...Da.":3954.0571,"Theo..MH...Da.":3954.05731,"DeltaM..ppm.":-0.05,"Deltam.z..Da.":-0.00007,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":3.73481,"Average.Reporter.S.N":6.5,"Ion.Inject.Time..ms.":22.626,"RT..min.":190.419,"First.Scan":97339,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_03.raw","File.ID":"F3","Abundance..126":1620.95903,"Abundance..127N":2633.29513,"Abundance..127C":3054.31326,"Abundance..128N":3053.75589,"Abundance..128C":1721.75808,"Abundance..129N":2661.74771,"Abundance..129C":2282.77104,"Abundance..130N":1893.32768,"Abundance..130C":2068.42092,"Abundance..131":2158.54788,"Ions.Score":91,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":6.01495e-09,"Percolator.q.Value":0,"Percolator.PEP":1.847e-10}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (L2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].lVEALcAAGHr.[A]","Modifications":"N-Term(TMT6plex); C6(Carbamidomethyl); R11(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P23919","Master.Protein.Descriptions":"Thymidylate kinase OS=Homo sapiens GN=DTYMK PE=1 SV=4","Protein.Accessions":"P23919","Protein.Descriptions":"Thymidylate kinase OS=Homo sapiens GN=DTYMK PE=1 SV=4","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":479.26831,"MH...Da.":1435.79038,"Theo..MH...Da.":1435.79165,"DeltaM..ppm.":-0.89,"Deltam.z..Da.":-0.00042,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":46.59391,"Average.Reporter.S.N":38.6,"Ion.Inject.Time..ms.":13.219,"RT..min.":103.3211,"First.Scan":48838,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_02.raw","File.ID":"F4","Abundance..126":49651.10422,"Abundance..127N":46884.94114,"Abundance..127C":56454.29844,"Abundance..128N":44876.33934,"Abundance..128C":47247.64724,"Abundance..129N":45011.48559,"Abundance..129C":53764.44172,"Abundance..130N":46937.74852,"Abundance..130C":42754.95669,"Abundance..131":50046.10234,"Ions.Score":26,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":0.01946,"Percolator.q.Value":0.00004,"Percolator.PEP":0.00042}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (L2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].sESEIVPk.[V]","Modifications":"N-Term(TMT6plex); K8(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q9Y450","Master.Protein.Descriptions":"HBS1-like protein OS=Homo sapiens GN=HBS1L PE=1 SV=1","Protein.Accessions":"Q9Y450","Protein.Descriptions":"HBS1-like protein OS=Homo sapiens GN=HBS1L PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":0.9048,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":677.90698,"MH...Da.":1354.80669,"Theo..MH...Da.":1354.80735,"DeltaM..ppm.":-0.49,"Deltam.z..Da.":-0.00033,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":18.73905,"Average.Reporter.S.N":19.9,"Ion.Inject.Time..ms.":50,"RT..min.":89.6275,"First.Scan":41255,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_02.raw","File.ID":"F4","Abundance..126":5898.59781,"Abundance..127N":6499.62637,"Abundance..127C":6826.67122,"Abundance..128N":6947.59669,"Abundance..128C":6113.9446,"Abundance..129N":7748.11758,"Abundance..129C":7992.64337,"Abundance..130N":7102.6503,"Abundance..130C":7118.38165,"Abundance..131":6760.56054,"Ions.Score":21,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.06493,"Percolator.q.Value":0.00004,"Percolator.PEP":0.00077}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (E2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].vALTGLTVAEYFr.[D]","Modifications":"N-Term(TMT6plex); R13(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":560.32452,"MH...Da.":1678.95902,"Theo..MH...Da.":1678.96049,"DeltaM..ppm.":-0.88,"Deltam.z..Da.":-0.00049,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":60.75401,"Average.Reporter.S.N":48.8,"Ion.Inject.Time..ms.":39.99,"RT..min.":182.615,"First.Scan":92157,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_01.raw","File.ID":"F11","Abundance..126":24456.62916,"Abundance..127N":22870.0987,"Abundance..127C":25276.30214,"Abundance..128N":25840.3178,"Abundance..128C":29285.37147,"Abundance..129N":23070.91806,"Abundance..129C":30597.7243,"Abundance..130N":24557.49182,"Abundance..130C":27812.10047,"Abundance..131":26736.30182,"Ions.Score":33,"Identity.Strict":30,"Identity.Relaxed":23,"Expectation.Value":0.00511,"Percolator.q.Value":0.00004,"Percolator.PEP":0.00013}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (E2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].vIISAPSADAPmFVMGVNHEk.[Y]","Modifications":"N-Term(TMT6plex); M12(Oxidation); K21(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.1389,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":899.15546,"MH...Da.":2695.45182,"Theo..MH...Da.":2695.4443,"DeltaM..ppm.":2.79,"Deltam.z..Da.":0.00251,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":32.54528,"Average.Reporter.S.N":10.6,"Ion.Inject.Time..ms.":30.149,"RT..min.":159.0087,"First.Scan":78891,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_01.raw","File.ID":"F11","Abundance..126":5889.03892,"Abundance..127N":7641.99218,"Abundance..127C":8538.04505,"Abundance..128N":6758.91334,"Abundance..128C":7399.52669,"Abundance..129N":10073.34516,"Abundance..129C":8628.25926,"Abundance..130N":5639.75553,"Abundance..130C":7563.03005,"Abundance..131":7528.79967,"Ions.Score":36,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.00206,"Percolator.q.Value":0.00004,"Percolator.PEP":0.00002}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (E2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[R].iLEQQNSSr.[T]","Modifications":"N-Term(TMT6plex); R9(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q16181","Master.Protein.Descriptions":"Septin-7 OS=Homo sapiens GN=SEPT7 PE=1 SV=2","Protein.Accessions":"Q16181","Protein.Descriptions":"Septin-7 OS=Homo sapiens GN=SEPT7 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":0.7556,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":657.36694,"MH...Da.":1313.72661,"Theo..MH...Da.":1313.72501,"DeltaM..ppm.":1.22,"Deltam.z..Da.":0.0008,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":0,"Average.Reporter.S.N":16.6,"Ion.Inject.Time..ms.":24.839,"RT..min.":59.991,"First.Scan":24521,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_01.raw","File.ID":"F11","Abundance..126":5676.09735,"Abundance..127N":5709.00394,"Abundance..127C":6796.18843,"Abundance..128N":5793.7785,"Abundance..128C":6749.98739,"Abundance..129N":5450.53999,"Abundance..129C":6553.7469,"Abundance..130N":6060.41403,"Abundance..130C":6440.41743,"Abundance..131":6407.29277,"Ions.Score":45,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.00025,"Percolator.q.Value":0.00004,"Percolator.PEP":0.00002}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (E2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].trEGNDLYHEMIESGVINLk.[D]","Modifications":"N-Term(TMT6plex); R2(Label:13C(6)15N(4)); K20(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":1,"Charge":4,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":699.37878,"MH...Da.":2794.49331,"Theo..MH...Da.":2794.49285,"DeltaM..ppm.":0.16,"Deltam.z..Da.":0.00011,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":22.40996,"Average.Reporter.S.N":25.5,"Ion.Inject.Time..ms.":4.107,"RT..min.":156.849,"First.Scan":77690,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_01.raw","File.ID":"F11","Abundance..126":62519.79839,"Abundance..127N":63162.07694,"Abundance..127C":81178.20062,"Abundance..128N":79602.58519,"Abundance..128C":75723.37981,"Abundance..129N":47912.25162,"Abundance..129C":65834.48992,"Abundance..130N":52924.58648,"Abundance..130C":70054.94814,"Abundance..131":65349.55392,"Ions.Score":5,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":3.27343,"Percolator.q.Value":0.00004,"Percolator.PEP":0.00004}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (E2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].wGDAGAEYVVESTGVFTTmEk.[A]","Modifications":"N-Term(TMT6plex); M19(Oxidation); K21(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":920.46545,"MH...Da.":2759.38181,"Theo..MH...Da.":2759.37297,"DeltaM..ppm.":3.2,"Deltam.z..Da.":0.00295,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":44.0515,"Average.Reporter.S.N":15.4,"Ion.Inject.Time..ms.":19.73,"RT..min.":193.7106,"First.Scan":98029,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_01.raw","File.ID":"F11","Abundance..126":8353.40461,"Abundance..127N":10703.2636,"Abundance..127C":10406.63384,"Abundance..128N":9451.42206,"Abundance..128C":8875.72408,"Abundance..129N":10041.4623,"Abundance..129C":11858.682,"Abundance..130N":9342.82611,"Abundance..130C":10548.39946,"Abundance..131":10768.22975,"Ions.Score":27,"Identity.Strict":26,"Identity.Relaxed":19,"Expectation.Value":0.00784,"Percolator.q.Value":0.00004,"Percolator.PEP":0.00002}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (D2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[K].lEMEMEAAr.[H]","Modifications":"N-Term(TMT6plex); R9(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q15233","Master.Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","Protein.Accessions":"Q15233","Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":0.9677,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":659.83246,"MH...Da.":1318.65764,"Theo..MH...Da.":1318.65719,"DeltaM..ppm.":0.34,"Deltam.z..Da.":0.00022,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":16.19012,"Average.Reporter.S.N":16.2,"Ion.Inject.Time..ms.":2.103,"RT..min.":114.1847,"First.Scan":56404,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_02.raw","File.ID":"F12","Abundance..126":144366.9129,"Abundance..127N":139589.78686,"Abundance..127C":136297.91404,"Abundance..128N":108671.89747,"Abundance..128C":145685.462,"Abundance..129N":125824.01964,"Abundance..129C":137309.74224,"Abundance..130N":139230.77621,"Abundance..130C":128009.60556,"Abundance..131":171433.14794,"Ions.Score":31,"Identity.Strict":23,"Identity.Relaxed":16,"Expectation.Value":0.00202,"Percolator.q.Value":0.00004,"Percolator.PEP":0.00033}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (C2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].lEMEMEAAr.[H]","Modifications":"N-Term(TMT6plex); R9(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q15233","Master.Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","Protein.Accessions":"Q15233","Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":659.83264,"MH...Da.":1318.65801,"Theo..MH...Da.":1318.65719,"DeltaM..ppm.":0.62,"Deltam.z..Da.":0.00041,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":20.77369,"Average.Reporter.S.N":22.1,"Ion.Inject.Time..ms.":3.198,"RT..min.":114.8054,"First.Scan":56638,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture4_01.raw","File.ID":"F13","Abundance..126":101816.02633,"Abundance..127N":95293.55236,"Abundance..127C":65709.1917,"Abundance..128N":70015.27368,"Abundance..128C":84035.43495,"Abundance..129N":82273.03509,"Abundance..129C":87447.98489,"Abundance..130N":77234.4145,"Abundance..130C":75932.73336,"Abundance..131":75057.55245,"Ions.Score":35,"Identity.Strict":23,"Identity.Relaxed":16,"Expectation.Value":0.00081,"Percolator.q.Value":0.00005,"Percolator.PEP":0.00033}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (C2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].qFAPIHAEAPEFMEmSVEQEILVTGIk.[V]","Modifications":"N-Term(TMT6plex); M15(Oxidation); K27(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":4,"DeltaScore":0.3,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":882.4668,"MH...Da.":3526.84536,"Theo..MH...Da.":3526.84573,"DeltaM..ppm.":-0.11,"Deltam.z..Da.":-0.00009,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":43.32654,"Average.Reporter.S.N":6.2,"Ion.Inject.Time..ms.":9.018,"RT..min.":185.3383,"First.Scan":96481,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture4_01.raw","File.ID":"F13","Abundance..126":15501.5771,"Abundance..127N":11526.55508,"Abundance..127C":6208.92463,"Abundance..128N":13835.7685,"Abundance..128C":12172.76457,"Abundance..129N":14642.07162,"Abundance..129C":9915.01442,"Abundance..130N":9812.93729,"Abundance..130C":9139.03342,"Abundance..131":19540.83804,"Ions.Score":20,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":0.0686,"Percolator.q.Value":0.00005,"Percolator.PEP":0.00017}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (I2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].sPLAQMEEErr.[E]","Modifications":"N-Term(TMT6plex); R10(Label:13C(6)15N(4)); R11(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q16181","Master.Protein.Descriptions":"Septin-7 OS=Homo sapiens GN=SEPT7 PE=1 SV=2","Protein.Accessions":"Q16181","Protein.Descriptions":"Septin-7 OS=Homo sapiens GN=SEPT7 PE=1 SV=2","X..Missed.Cleavages":1,"Charge":3,"DeltaScore":0.9796,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":532.2829,"MH...Da.":1594.83414,"Theo..MH...Da.":1594.83234,"DeltaM..ppm.":1.13,"Deltam.z..Da.":0.0006,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":9.88153,"Average.Reporter.S.N":17.3,"Ion.Inject.Time..ms.":5.695,"RT..min.":74.4361,"First.Scan":35000,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_01.raw","File.ID":"F7","Abundance..126":50749.55189,"Abundance..127N":40017.20466,"Abundance..127C":39330.46217,"Abundance..128N":36639.2689,"Abundance..128C":42797.25726,"Abundance..129N":50394.3977,"Abundance..129C":37374.03268,"Abundance..130N":26174.50738,"Abundance..130C":33246.96021,"Abundance..131":41938.36341,"Ions.Score":49,"Identity.Strict":27,"Identity.Relaxed":20,"Expectation.Value":0.00007,"Percolator.q.Value":0.00006,"Percolator.PEP":0.00018}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (I2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].vALVYGQMNEPPGAr.[A]","Modifications":"N-Term(TMT6plex); R15(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":920.9964,"MH...Da.":1840.98552,"Theo..MH...Da.":1840.98164,"DeltaM..ppm.":2.11,"Deltam.z..Da.":0.00194,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":0,"Average.Reporter.S.N":21,"Ion.Inject.Time..ms.":6.747,"RT..min.":135.4647,"First.Scan":71155,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_01.raw","File.ID":"F7","Abundance..126":19967.47416,"Abundance..127N":18434.91904,"Abundance..127C":16939.66948,"Abundance..128N":17116.37047,"Abundance..128C":19950.24727,"Abundance..129N":12846.13317,"Abundance..129C":19866.45202,"Abundance..130N":20377.55957,"Abundance..130C":20748.3943,"Abundance..131":19762.96779,"Ions.Score":25,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.02842,"Percolator.q.Value":0.00006,"Percolator.PEP":0.00024}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (I2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].fTQAGSEVSALLGr.[I]","Modifications":"N-Term(TMT6plex); R14(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":837.96661,"MH...Da.":1674.92595,"Theo..MH...Da.":1674.92517,"DeltaM..ppm.":0.47,"Deltam.z..Da.":0.00039,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":27.78627,"Average.Reporter.S.N":17.1,"Ion.Inject.Time..ms.":2.918,"RT..min.":161.3823,"First.Scan":86648,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_01.raw","File.ID":"F7","Abundance..126":100873.55365,"Abundance..127N":87942.68696,"Abundance..127C":114126.88542,"Abundance..128N":104269.61188,"Abundance..128C":117448.25235,"Abundance..129N":87204.69855,"Abundance..129C":122953.55159,"Abundance..130N":79245.24684,"Abundance..130C":89512.20887,"Abundance..131":119658.34739,"Ions.Score":24,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.04294,"Percolator.q.Value":0.00006,"Percolator.PEP":0.00014}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (I2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[K].kGSITSVQAIYVPADDLTDPAPATTFAHLDATTVLSr.[A]","Modifications":"N-Term(TMT6plex); K1(Label); R37(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":1,"Charge":4,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":1080.58862,"MH...Da.":4319.33266,"Theo..MH...Da.":4319.3294,"DeltaM..ppm.":0.75,"Deltam.z..Da.":0.00081,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":0,"Average.Reporter.S.N":3.7,"Ion.Inject.Time..ms.":1.336,"RT..min.":176.782,"First.Scan":95734,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_01.raw","File.ID":"F7","Abundance..126":7904.80726,"Abundance..127N":8604.3336,"Abundance..127C":10650.75773,"Abundance..128N":12379.65331,"Abundance..128C":14009.46729,"Abundance..129N":12663.04389,"Abundance..129C":14963.46005,"Abundance..130N":6765.81302,"Abundance..131":12451.03468,"Ions.Score":40,"Identity.Strict":27,"Identity.Relaxed":20,"Expectation.Value":0.00055,"Percolator.q.Value":0.00006,"Percolator.PEP":0.00023}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (I2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[R].gAMPPAPVPAGTPAPPGPATMMPDGTLGLTPPTTEr.[F]","Modifications":"N-Term(TMT6plex); R36(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q15233","Master.Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","Protein.Accessions":"Q15233","Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":1230.96167,"MH...Da.":3690.87046,"Theo..MH...Da.":3690.87704,"DeltaM..ppm.":-1.78,"Deltam.z..Da.":-0.0022,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":0,"Average.Reporter.S.N":7.1,"Ion.Inject.Time..ms.":26.994,"RT..min.":182.0942,"First.Scan":98837,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_01.raw","File.ID":"F7","Abundance..126":3011.46746,"Abundance..127N":3222.24302,"Abundance..127C":4444.64625,"Abundance..128N":2755.31001,"Abundance..128C":2383.5487,"Abundance..129N":1929.21115,"Abundance..129C":3232.90028,"Abundance..130N":3972.60311,"Abundance..130C":3665.42559,"Abundance..131":3129.95472,"Ions.Score":46,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":0.00019,"Percolator.q.Value":0.00006,"Percolator.PEP":0.00018}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (I2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].vIHDNFGIVEGLMTTVHAITATQk.[T]","Modifications":"N-Term(TMT6plex); K24(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":0,"Charge":5,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":613.14508,"MH...Da.":3061.6963,"Theo..MH...Da.":3061.7,"DeltaM..ppm.":-1.21,"Deltam.z..Da.":-0.00074,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":6.46873,"Average.Reporter.S.N":20.2,"Ion.Inject.Time..ms.":1.254,"RT..min.":191.9691,"First.Scan":104588,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_01.raw","File.ID":"F7","Abundance..126":238400.67774,"Abundance..127N":239344.62191,"Abundance..127C":233368.99724,"Abundance..128N":254180.11545,"Abundance..128C":267681.66376,"Abundance..129N":249190.5896,"Abundance..129C":272591.57759,"Abundance..130N":207679.92217,"Abundance..130C":190745.31743,"Abundance..131":185354.32794,"Ions.Score":26,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.02165,"Percolator.q.Value":0.00006,"Percolator.PEP":0.00024}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (K2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].lFVGNLPPDITEEEMr.[K]","Modifications":"N-Term(TMT6plex); R16(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q15233","Master.Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","Protein.Accessions":"Q15233","Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":1050.04919,"MH...Da.":2099.09111,"Theo..MH...Da.":2099.09198,"DeltaM..ppm.":-0.41,"Deltam.z..Da.":-0.00043,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":0,"Average.Reporter.S.N":15.4,"Ion.Inject.Time..ms.":11.402,"RT..min.":176.9193,"First.Scan":94545,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_03.raw","File.ID":"F5","Abundance..126":15978.80114,"Abundance..127N":14642.55696,"Abundance..127C":14095.95682,"Abundance..128N":12911.40708,"Abundance..128C":8387.46878,"Abundance..129N":13429.83314,"Abundance..129C":15360.08723,"Abundance..130N":16207.90112,"Abundance..130C":13218.04689,"Abundance..131":11898.08346,"Ions.Score":22,"Identity.Strict":27,"Identity.Relaxed":20,"Expectation.Value":0.03435,"Percolator.q.Value":0.00007,"Percolator.PEP":0.00021}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (K2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].vVDLMAHMASk.[E]","Modifications":"N-Term(TMT6plex); K11(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.963,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":556.65399,"MH...Da.":1667.94742,"Theo..MH...Da.":1667.94684,"DeltaM..ppm.":0.35,"Deltam.z..Da.":0.00019,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":0,"Average.Reporter.S.N":215.8,"Ion.Inject.Time..ms.":2.747,"RT..min.":146.7845,"First.Scan":76742,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_03.raw","File.ID":"F5","Abundance..126":6592345.42988,"Abundance..127N":7029241.56683,"Abundance..127C":6727571.61953,"Abundance..128N":7081096.37245,"Abundance..128C":6545744.21371,"Abundance..129N":6471837.80612,"Abundance..129C":7335886.84976,"Abundance..130N":7055199.08836,"Abundance..130C":6767697.58292,"Abundance..131":6882677.51574,"Ions.Score":27,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.01897,"Percolator.q.Value":0.00007,"Percolator.PEP":0.00021}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (K2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].lVINGNPITIFQErDPSk.[I]","Modifications":"N-Term(TMT6plex); R14(Label:13C(6)15N(4)); K18(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":1,"Charge":3,"DeltaScore":0.8372,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":839.82416,"MH...Da.":2517.45792,"Theo..MH...Da.":2517.456,"DeltaM..ppm.":0.76,"Deltam.z..Da.":0.00064,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":0.40614,"Average.Reporter.S.N":33.3,"Ion.Inject.Time..ms.":0.186,"RT..min.":165.9444,"First.Scan":88100,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_03.raw","File.ID":"F5","Abundance..126":3324137.00875,"Abundance..127N":3203031.24293,"Abundance..127C":3129928.99804,"Abundance..128N":3051557.21558,"Abundance..128C":3005550.05694,"Abundance..129N":2961029.58916,"Abundance..129C":2924926.66364,"Abundance..130N":2614986.99375,"Abundance..130C":3003931.76167,"Abundance..131":2963431.16765,"Ions.Score":43,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.00047,"Percolator.q.Value":0.00007,"Percolator.PEP":0.0002}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (K2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].cSEGSFLLTTFPrPVTVEPMDQLDDEEGLPEk.[L]","Modifications":"N-Term(TMT6plex); C1(Carbamidomethyl); R13(Label:13C(6)15N(4)); K32(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q15233","Master.Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","Protein.Accessions":"Q15233","Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","X..Missed.Cleavages":1,"Charge":4,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":1029.02026,"MH...Da.":4113.05922,"Theo..MH...Da.":4113.05674,"DeltaM..ppm.":0.6,"Deltam.z..Da.":0.00062,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":2.97867,"Average.Reporter.S.N":26,"Ion.Inject.Time..ms.":3.543,"RT..min.":187.9491,"First.Scan":100919,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_03.raw","File.ID":"F5","Abundance..126":379568.48572,"Abundance..127N":282151.88321,"Abundance..127C":326713.27478,"Abundance..128N":342518.27246,"Abundance..128C":337498.62431,"Abundance..129N":295421.76589,"Abundance..129C":383635.19943,"Abundance..130N":344763.07431,"Abundance..130C":312294.36464,"Abundance..131":380231.72399,"Ions.Score":31,"Identity.Strict":26,"Identity.Relaxed":19,"Expectation.Value":0.00382,"Percolator.q.Value":0.00007,"Percolator.PEP":0.00017}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (K2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].rDLLFQALGr.[T]","Modifications":"N-Term(TMT6plex); R1(Label:13C(6)15N(4)); R10(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q9NSD9","Master.Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","Protein.Accessions":"Q9NSD9","Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","X..Missed.Cleavages":1,"Charge":3,"DeltaScore":0.9318,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":479.9595,"MH...Da.":1437.86396,"Theo..MH...Da.":1437.86424,"DeltaM..ppm.":-0.2,"Deltam.z..Da.":-0.00009,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":29.9808,"Average.Reporter.S.N":26.9,"Ion.Inject.Time..ms.":6.631,"RT..min.":135.9854,"First.Scan":70385,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_03.raw","File.ID":"F5","Abundance..126":21323.61944,"Abundance..127N":19193.48482,"Abundance..127C":21623.47408,"Abundance..128N":24627.47261,"Abundance..128C":22190.20119,"Abundance..129N":20781.81706,"Abundance..129C":28389.50384,"Abundance..130N":21758.27887,"Abundance..130C":22423.43007,"Abundance..131":22471.92711,"Ions.Score":44,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.00032,"Percolator.q.Value":0.00007,"Percolator.PEP":0.00019}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (K2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].vIISAPSADAPmFVMGVNHEk.[Y]","Modifications":"N-Term(TMT6plex); M12(Oxidation); K21(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.2647,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":899.15363,"MH...Da.":2695.44632,"Theo..MH...Da.":2695.4443,"DeltaM..ppm.":0.75,"Deltam.z..Da.":0.00067,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":20.88109,"Average.Reporter.S.N":17.5,"Ion.Inject.Time..ms.":5.776,"RT..min.":154.6976,"First.Scan":81438,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_03.raw","File.ID":"F5","Abundance..126":17754.85495,"Abundance..127N":24791.53133,"Abundance..127C":19648.60937,"Abundance..128N":22763.45919,"Abundance..128C":23752.53754,"Abundance..129N":20601.59466,"Abundance..129C":20579.6812,"Abundance..130N":17755.65506,"Abundance..130C":25162.6174,"Abundance..131":25694.33788,"Ions.Score":34,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.00342,"Percolator.q.Value":0.00007,"Percolator.PEP":0.00016}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (K2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[K].tVLIMELINNVAk.[A]","Modifications":"N-Term(TMT6plex); K13(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.8966,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":642.06525,"MH...Da.":1924.18119,"Theo..MH...Da.":1924.17968,"DeltaM..ppm.":0.79,"Deltam.z..Da.":0.0005,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":20.32257,"Average.Reporter.S.N":101.2,"Ion.Inject.Time..ms.":8.939,"RT..min.":208.6376,"First.Scan":112382,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_03.raw","File.ID":"F5","Abundance..126":203338.34687,"Abundance..127N":191273.5831,"Abundance..127C":188222.58278,"Abundance..128N":211737.87656,"Abundance..128C":191455.16704,"Abundance..129N":200565.73126,"Abundance..129C":226774.77228,"Abundance..130N":206282.52174,"Abundance..130C":201354.29527,"Abundance..131":188168.04849,"Ions.Score":29,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":0.00844,"Percolator.q.Value":0.00007,"Percolator.PEP":0.00018}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (K2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].irPFAVAAVLr.[N]","Modifications":"N-Term(TMT6plex); R2(Label:13C(6)15N(4)); R11(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q9NSD9","Master.Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","Protein.Accessions":"Q9NSD9","Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","X..Missed.Cleavages":1,"Charge":3,"DeltaScore":0.9615,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":487.98361,"MH...Da.":1461.93628,"Theo..MH...Da.":1461.93701,"DeltaM..ppm.":-0.5,"Deltam.z..Da.":-0.00024,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":12.07499,"Average.Reporter.S.N":31.4,"Ion.Inject.Time..ms.":2.928,"RT..min.":134.3365,"First.Scan":69413,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_03.raw","File.ID":"F5","Abundance..126":140919.38533,"Abundance..127N":152704.73733,"Abundance..127C":150246.46677,"Abundance..128N":147376.53555,"Abundance..128C":164018.7432,"Abundance..129N":163122.03632,"Abundance..129C":150484.04218,"Abundance..130N":159284.86687,"Abundance..130C":191757.82055,"Abundance..131":156035.05957,"Ions.Score":52,"Identity.Strict":27,"Identity.Relaxed":20,"Expectation.Value":0.00004,"Percolator.q.Value":0.00007,"Percolator.PEP":0.00019}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (K2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].wGDAGAEYVVESTGVFTTMEk.[A]","Modifications":"N-Term(TMT6plex); K21(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":915.13263,"MH...Da.":2743.38333,"Theo..MH...Da.":2743.37805,"DeltaM..ppm.":1.93,"Deltam.z..Da.":0.00176,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":26.46472,"Average.Reporter.S.N":0.3,"Ion.Inject.Time..ms.":50,"RT..min.":246.6567,"First.Scan":130445,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_03.raw","File.ID":"F5","Abundance..127C":484.93326,"Abundance..128N":465.95163,"Ions.Score":31,"Identity.Strict":27,"Identity.Relaxed":20,"Expectation.Value":0.00469,"Percolator.q.Value":0.00007,"Percolator.PEP":0.0002}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (E2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].tAEFQVAr.[T]","Modifications":"N-Term(TMT6plex); R8(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q9NSD9","Master.Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","Protein.Accessions":"Q9NSD9","Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":0.9583,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":580.8288,"MH...Da.":1160.65032,"Theo..MH...Da.":1160.65006,"DeltaM..ppm.":0.22,"Deltam.z..Da.":0.00013,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":34.98402,"Average.Reporter.S.N":13.6,"Ion.Inject.Time..ms.":8.956,"RT..min.":78.8613,"First.Scan":34494,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_01.raw","File.ID":"F11","Abundance..126":10843.606,"Abundance..127N":13524.78964,"Abundance..127C":13118.88127,"Abundance..128N":16623.61415,"Abundance..128C":17650.5081,"Abundance..129N":21215.82956,"Abundance..129C":12169.96449,"Abundance..130N":11390.61657,"Abundance..130C":15251.39863,"Abundance..131":16824.55151,"Ions.Score":24,"Identity.Strict":26,"Identity.Relaxed":19,"Expectation.Value":0.01605,"Percolator.q.Value":0.00008,"Percolator.PEP":0.00068}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (D2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[K].nLEGYVGFANLPNQVYr.[K]","Modifications":"N-Term(TMT6plex); R17(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q16181","Master.Protein.Descriptions":"Septin-7 OS=Homo sapiens GN=SEPT7 PE=1 SV=2","Protein.Accessions":"Q16181","Protein.Descriptions":"Septin-7 OS=Homo sapiens GN=SEPT7 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":731.72308,"MH...Da.":2193.1547,"Theo..MH...Da.":2193.15294,"DeltaM..ppm.":0.8,"Deltam.z..Da.":0.00059,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":2.01829,"Average.Reporter.S.N":10.5,"Ion.Inject.Time..ms.":1.588,"RT..min.":169.7944,"First.Scan":89196,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_02.raw","File.ID":"F12","Abundance..126":52312.49035,"Abundance..127N":42585.32587,"Abundance..127C":54197.75967,"Abundance..128N":33892.85411,"Abundance..128C":39485.59448,"Abundance..129N":41525.13199,"Abundance..129C":37655.41181,"Abundance..130N":47879.03616,"Abundance..130C":44265.71415,"Abundance..131":34458.44097,"Ions.Score":19,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":0.08714,"Percolator.q.Value":0.00008,"Percolator.PEP":0.00067}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (D2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].mEELHNQEVQk.[R]","Modifications":"N-Term(TMT6plex); K11(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q15233","Master.Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","Protein.Accessions":"Q15233","Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":617.66937,"MH...Da.":1850.99356,"Theo..MH...Da.":1850.9926,"DeltaM..ppm.":0.52,"Deltam.z..Da.":0.00032,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":11.16962,"Average.Reporter.S.N":54.7,"Ion.Inject.Time..ms.":10.628,"RT..min.":79.6819,"First.Scan":36379,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_02.raw","File.ID":"F12","Abundance..126":32507.99038,"Abundance..127N":29711.08156,"Abundance..127C":36183.64492,"Abundance..128N":44216.20475,"Abundance..128C":35540.1403,"Abundance..129N":41663.81124,"Abundance..129C":43005.72659,"Abundance..130N":36518.26842,"Abundance..130C":37056.34752,"Abundance..131":37701.51934,"Ions.Score":21,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.07447,"Percolator.q.Value":0.00008,"Percolator.PEP":0.00095}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (D2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].vIHDNFGIVEGLmTTVHAITATQk.[T]","Modifications":"N-Term(TMT6plex); M13(Oxidation); K24(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":0,"Charge":4,"DeltaScore":0.8333,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":770.18048,"MH...Da.":3077.70009,"Theo..MH...Da.":3077.69491,"DeltaM..ppm.":1.68,"Deltam.z..Da.":0.0013,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":38.92744,"Average.Reporter.S.N":10.6,"Ion.Inject.Time..ms.":13.155,"RT..min.":198.1816,"First.Scan":105430,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_02.raw","File.ID":"F12","Abundance..126":9829.11132,"Abundance..127N":8629.75807,"Abundance..127C":9710.35411,"Abundance..128N":7993.52784,"Abundance..128C":8123.99802,"Abundance..129N":8536.21192,"Abundance..129C":10050.69657,"Abundance..130N":8149.65679,"Abundance..130C":13228.83148,"Abundance..131":7863.48022,"Ions.Score":6,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":2.21448,"Percolator.q.Value":0.00008,"Percolator.PEP":0.00059}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (D2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[R].vVDLMAHmASk.[E]","Modifications":"N-Term(TMT6plex); M8(Oxidation); K11(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":561.98615,"MH...Da.":1683.94388,"Theo..MH...Da.":1683.94175,"DeltaM..ppm.":1.26,"Deltam.z..Da.":0.00071,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":27.60517,"Average.Reporter.S.N":37.2,"Ion.Inject.Time..ms.":1.636,"RT..min.":110.4651,"First.Scan":54196,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_02.raw","File.ID":"F12","Abundance..126":295633.86691,"Abundance..127N":282343.88393,"Abundance..127C":221124.2539,"Abundance..128N":289231.44924,"Abundance..128C":267199.19157,"Abundance..129N":300165.20825,"Abundance..129C":222008.44483,"Abundance..130N":232371.76893,"Abundance..130C":255493.80486,"Abundance..131":281393.24201,"Ions.Score":20,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.10895,"Percolator.q.Value":0.00008,"Percolator.PEP":0.00106}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (D2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].smTEAEQQQLIDDHFLFDk.[P]","Modifications":"N-Term(TMT6plex); M2(Oxidation); K19(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P12277","Master.Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","Protein.Accessions":"P12277","Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.9231,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":926.47034,"MH...Da.":2777.39646,"Theo..MH...Da.":2777.39477,"DeltaM..ppm.":0.61,"Deltam.z..Da.":0.00056,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":0,"Average.Reporter.S.N":6.8,"Ion.Inject.Time..ms.":7.881,"RT..min.":165.8492,"First.Scan":86889,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_02.raw","File.ID":"F12","Abundance..126":7790.08374,"Abundance..127N":6762.04259,"Abundance..127C":7972.07707,"Abundance..128N":8052.5318,"Abundance..128C":9734.12335,"Abundance..129N":9440.2637,"Abundance..129C":6689.81496,"Abundance..130N":9924.97159,"Abundance..130C":6427.78287,"Abundance..131":8682.72891,"Ions.Score":26,"Identity.Strict":27,"Identity.Relaxed":20,"Expectation.Value":0.01533,"Percolator.q.Value":0.00008,"Percolator.PEP":0.00113}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (N2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].lGFSEVELVQmVVDGVk.[L]","Modifications":"N-Term(TMT6plex); M11(Oxidation); K17(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P12277","Master.Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","Protein.Accessions":"P12277","Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.5667,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":777.77679,"MH...Da.":2331.31583,"Theo..MH...Da.":2331.31254,"DeltaM..ppm.":1.41,"Deltam.z..Da.":0.0011,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":9.03679,"Average.Reporter.S.N":24.6,"Ion.Inject.Time..ms.":14.685,"RT..min.":208.5222,"First.Scan":108440,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_03.raw","File.ID":"F2","Abundance..126":12111.24163,"Abundance..127N":11087.91985,"Abundance..127C":13971.59593,"Abundance..128N":12878.90846,"Abundance..128C":12129.71222,"Abundance..129N":12021.01022,"Abundance..129C":13967.51006,"Abundance..130N":14508.14071,"Abundance..130C":11566.94692,"Abundance..131":13348.28324,"Ions.Score":30,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.00914,"Percolator.q.Value":0.00009,"Percolator.PEP":0.00053}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (N2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].iMNVIGEPIDEr.[G]","Modifications":"N-Term(TMT6plex); R12(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":812.94519,"MH...Da.":1624.8831,"Theo..MH...Da.":1624.88053,"DeltaM..ppm.":1.59,"Deltam.z..Da.":0.00129,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":19.78375,"Average.Reporter.S.N":26.2,"Ion.Inject.Time..ms.":6.782,"RT..min.":154.4962,"First.Scan":78407,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_03.raw","File.ID":"F2","Abundance..126":77272.89299,"Abundance..127N":73229.75037,"Abundance..127C":76221.72839,"Abundance..128N":84122.12968,"Abundance..128C":69973.87392,"Abundance..129N":63911.52635,"Abundance..129C":61475.66283,"Abundance..130N":71996.80636,"Abundance..130C":62877.50125,"Abundance..131":86978.31736,"Ions.Score":60,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":8.0738e-06,"Percolator.q.Value":0.00009,"Percolator.PEP":0.00019}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (H2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].lEMEMEAAr.[H]","Modifications":"N-Term(TMT6plex); R9(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q15233","Master.Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","Protein.Accessions":"Q15233","Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":659.83282,"MH...Da.":1318.65837,"Theo..MH...Da.":1318.65719,"DeltaM..ppm.":0.89,"Deltam.z..Da.":0.00059,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":31.88243,"Average.Reporter.S.N":19.4,"Ion.Inject.Time..ms.":3.123,"RT..min.":113.9775,"First.Scan":54567,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_02.raw","File.ID":"F8","Abundance..126":78981.41612,"Abundance..127N":91358.04699,"Abundance..127C":94238.5407,"Abundance..128N":96530.88378,"Abundance..128C":106946.90856,"Abundance..129N":83191.4242,"Abundance..129C":54776.60132,"Abundance..130N":67969.00245,"Abundance..130C":98233.32829,"Abundance..131":106058.74818,"Ions.Score":32,"Identity.Strict":23,"Identity.Relaxed":16,"Expectation.Value":0.0017,"Percolator.q.Value":0.0001,"Percolator.PEP":0.0004}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (H2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].rVIISAPSADAPMFVmGVNHEk.[Y]","Modifications":"N-Term(TMT6plex); R1(Label:13C(6)15N(4)); M16(Oxidation); K22(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":1,"Charge":4,"DeltaScore":0.3793,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":716.14459,"MH...Da.":2861.55654,"Theo..MH...Da.":2861.55368,"DeltaM..ppm.":1,"Deltam.z..Da.":0.00072,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":9.72175,"Average.Reporter.S.N":13.9,"Ion.Inject.Time..ms.":1.981,"RT..min.":131.1212,"First.Scan":64122,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_02.raw","File.ID":"F8","Abundance..126":16912.59661,"Abundance..127N":23658.77976,"Abundance..127C":22513.79158,"Abundance..128N":31198.70485,"Abundance..128C":20753.41276,"Abundance..129N":28167.31579,"Abundance..129C":26950.58838,"Abundance..130N":24827.64217,"Abundance..130C":19687.93024,"Abundance..131":26374.52297,"Ions.Score":29,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.01089,"Percolator.q.Value":0.0001,"Percolator.PEP":0.00041}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (A2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].iMNVIGEPIDEr.[G]","Modifications":"N-Term(TMT6plex); R12(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":812.94403,"MH...Da.":1624.88078,"Theo..MH...Da.":1624.88053,"DeltaM..ppm.":0.16,"Deltam.z..Da.":0.00013,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":100,"Average.Reporter.S.N":25.7,"Ion.Inject.Time..ms.":10.53,"RT..min.":155.8785,"First.Scan":78947,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_01.raw","File.ID":"F15","Abundance..126":66517.18053,"Abundance..127N":68208.6098,"Abundance..127C":67268.21056,"Abundance..128N":80909.02381,"Abundance..128C":84016.5499,"Abundance..129N":78025.042,"Abundance..129C":64294.7104,"Abundance..130N":81504.14515,"Abundance..130C":79009.01168,"Abundance..131":84580.09203,"Ions.Score":28,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":0.01123,"Percolator.q.Value":0.00012,"Percolator.PEP":0.00087}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (A2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].mEELHNQEVQk.[R]","Modifications":"N-Term(TMT6plex); K11(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q15233","Master.Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","Protein.Accessions":"Q15233","Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.871,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":617.6687,"MH...Da.":1850.99155,"Theo..MH...Da.":1850.9926,"DeltaM..ppm.":-0.57,"Deltam.z..Da.":-0.00035,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":9.18321,"Average.Reporter.S.N":19.5,"Ion.Inject.Time..ms.":6.184,"RT..min.":78.674,"First.Scan":34979,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_01.raw","File.ID":"F15","Abundance..126":44964.48835,"Abundance..127N":47174.52114,"Abundance..127C":49489.23925,"Abundance..128N":47111.15351,"Abundance..128C":36351.38951,"Abundance..129N":35167.81644,"Abundance..129C":58542.63095,"Abundance..130N":55176.15454,"Abundance..130C":54730.12707,"Abundance..131":43133.49117,"Ions.Score":31,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.00682,"Percolator.q.Value":0.00012,"Percolator.PEP":0.00075}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (H2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].lISWYDNEFGYSNr.[V]","Modifications":"N-Term(TMT6plex); R14(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":668.32983,"MH...Da.":2002.97495,"Theo..MH...Da.":2002.97358,"DeltaM..ppm.":0.69,"Deltam.z..Da.":0.00046,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":0,"Average.Reporter.S.N":18.4,"Ion.Inject.Time..ms.":15.013,"RT..min.":180.3753,"First.Scan":92109,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_02.raw","File.ID":"F8","Abundance..126":27809.16452,"Abundance..127N":29876.43394,"Abundance..127C":39294.25453,"Abundance..128N":26796.85044,"Abundance..128C":25817.63798,"Abundance..129N":24252.58995,"Abundance..129C":27833.49668,"Abundance..130N":33584.91954,"Abundance..130C":32637.88241,"Abundance..131":26997.9074,"Ions.Score":23,"Identity.Strict":24,"Identity.Relaxed":17,"Expectation.Value":0.01696,"Percolator.q.Value":0.00012,"Percolator.PEP":0.00082}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (H2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].rVIISAPSADAPMFVmGVNHEk.[Y]","Modifications":"N-Term(TMT6plex); R1(Label:13C(6)15N(4)); M16(Oxidation); K22(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":1,"Charge":4,"DeltaScore":0.6842,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":716.14484,"MH...Da.":2861.55752,"Theo..MH...Da.":2861.55368,"DeltaM..ppm.":1.34,"Deltam.z..Da.":0.00096,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":30.47201,"Average.Reporter.S.N":10.2,"Ion.Inject.Time..ms.":2.662,"RT..min.":145.2791,"First.Scan":72087,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_02.raw","File.ID":"F8","Abundance..126":46870.44284,"Abundance..127N":55521.97145,"Abundance..127C":61243.40069,"Abundance..128N":55765.79598,"Abundance..128C":30981.8307,"Abundance..129N":35085.32316,"Abundance..129C":42627.95946,"Abundance..130N":49884.78013,"Abundance..130C":39878.66734,"Abundance..131":89160.88649,"Ions.Score":19,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.12364,"Percolator.q.Value":0.00012,"Percolator.PEP":0.00075}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (H2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].cSEGSFLLTTFPrPVTVEPMDQLDDEEGLPEk.[L]","Modifications":"N-Term(TMT6plex); C1(Carbamidomethyl); R13(Label:13C(6)15N(4)); K32(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q15233","Master.Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","Protein.Accessions":"Q15233","Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","X..Missed.Cleavages":1,"Charge":4,"DeltaScore":0.88,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":1029.02161,"MH...Da.":4113.0646,"Theo..MH...Da.":4113.05674,"DeltaM..ppm.":1.91,"Deltam.z..Da.":0.00196,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":0,"Average.Reporter.S.N":8.6,"Ion.Inject.Time..ms.":3.72,"RT..min.":186.9883,"First.Scan":95728,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_02.raw","File.ID":"F8","Abundance..126":27462.31493,"Abundance..127N":12640.74102,"Abundance..127C":22944.3305,"Abundance..128N":14347.71583,"Abundance..128C":31530.73009,"Abundance..129N":20302.9987,"Abundance..129C":28772.27882,"Abundance..130N":19325.0469,"Abundance..130C":18888.42203,"Abundance..131":20186.79518,"Ions.Score":25,"Identity.Strict":26,"Identity.Relaxed":19,"Expectation.Value":0.01492,"Percolator.q.Value":0.00012,"Percolator.PEP":0.00057}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (H2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].lAVEALSSLDGDLAGr.[Y]","Modifications":"N-Term(TMT6plex); R16(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P12277","Master.Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","Protein.Accessions":"P12277","Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":913.50958,"MH...Da.":1826.01189,"Theo..MH...Da.":1826.00963,"DeltaM..ppm.":1.24,"Deltam.z..Da.":0.00113,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":8.88875,"Average.Reporter.S.N":26,"Ion.Inject.Time..ms.":4.386,"RT..min.":181.8086,"First.Scan":92918,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_02.raw","File.ID":"F8","Abundance..126":47901.14579,"Abundance..127N":64577.74467,"Abundance..127C":61963.14035,"Abundance..128N":59512.5072,"Abundance..128C":66517.69629,"Abundance..129N":62773.32418,"Abundance..129C":55076.98778,"Abundance..130N":56086.39582,"Abundance..130C":63965.17258,"Abundance..131":60585.60456,"Ions.Score":42,"Identity.Strict":30,"Identity.Relaxed":23,"Expectation.Value":0.00063,"Percolator.q.Value":0.00012,"Percolator.PEP":0.0006}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (H2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].lVINGNPITIFQErDPSk.[I]","Modifications":"N-Term(TMT6plex); R14(Label:13C(6)15N(4)); K18(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":1,"Charge":3,"DeltaScore":0.875,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":839.82452,"MH...Da.":2517.45902,"Theo..MH...Da.":2517.456,"DeltaM..ppm.":1.2,"Deltam.z..Da.":0.00101,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":0.22933,"Average.Reporter.S.N":15.3,"Ion.Inject.Time..ms.":0.801,"RT..min.":165.6704,"First.Scan":83812,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_02.raw","File.ID":"F8","Abundance..126":49184.24364,"Abundance..127N":48250.84498,"Abundance..127C":74967.31717,"Abundance..128N":86464.3955,"Abundance..128C":66831.96766,"Abundance..129N":57492.44217,"Abundance..129C":72021.81416,"Abundance..130N":62009.00968,"Abundance..130C":82152.79367,"Abundance..131":64999.0065,"Ions.Score":24,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.03556,"Percolator.q.Value":0.00012,"Percolator.PEP":0.0007}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (H2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].gQNALVELQTQrPIALELYk.[D]","Modifications":"N-Term(TMT6plex); R12(Label:13C(6)15N(4)); K20(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q9Y450","Master.Protein.Descriptions":"HBS1-like protein OS=Homo sapiens GN=HBS1L PE=1 SV=1","Protein.Accessions":"Q9Y450","Protein.Descriptions":"HBS1-like protein OS=Homo sapiens GN=HBS1L PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":920.8775,"MH...Da.":2760.61795,"Theo..MH...Da.":2760.61429,"DeltaM..ppm.":1.33,"Deltam.z..Da.":0.00122,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":34.30991,"Average.Reporter.S.N":16.4,"Ion.Inject.Time..ms.":17.471,"RT..min.":163.3658,"First.Scan":82485,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_02.raw","File.ID":"F8","Abundance..126":5130.65694,"Abundance..127N":5575.69611,"Abundance..127C":5828.6715,"Abundance..128N":5913.09296,"Abundance..128C":5893.38687,"Abundance..129N":5354.54236,"Abundance..129C":4157.43276,"Abundance..130N":4956.38755,"Abundance..130C":6413.79808,"Abundance..131":4881.25219,"Ions.Score":9,"Identity.Strict":27,"Identity.Relaxed":20,"Expectation.Value":0.68182,"Percolator.q.Value":0.00012,"Percolator.PEP":0.00058}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (J2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].hLcAVYYNk.[N]","Modifications":"N-Term(TMT6plex); C3(Carbamidomethyl); K9(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q9NSD9","Master.Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","Protein.Accessions":"Q9NSD9","Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":545.30371,"MH...Da.":1633.89658,"Theo..MH...Da.":1633.9016,"DeltaM..ppm.":-3.07,"Deltam.z..Da.":-0.00167,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":17.25873,"Average.Reporter.S.N":92,"Ion.Inject.Time..ms.":5.193,"RT..min.":85.8402,"First.Scan":40449,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture4_03.raw","File.ID":"F6","Abundance..126":159537.7628,"Abundance..127N":187250.53279,"Abundance..127C":158510.55778,"Abundance..128N":184758.74137,"Abundance..128C":164513.23572,"Abundance..129N":176053.39138,"Abundance..129C":184905.74167,"Abundance..130N":158723.60158,"Abundance..130C":160891.0828,"Abundance..131":170653.21047,"Ions.Score":23,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":0.03657,"Percolator.q.Value":0.00012,"Percolator.PEP":0.00142}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (N2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[K].vLTPELYAELr.[A]","Modifications":"N-Term(TMT6plex); R11(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P12277","Master.Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","Protein.Accessions":"P12277","Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":0.9167,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":771.95258,"MH...Da.":1542.89787,"Theo..MH...Da.":1542.89683,"DeltaM..ppm.":0.68,"Deltam.z..Da.":0.00052,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":12.66776,"Average.Reporter.S.N":24.4,"Ion.Inject.Time..ms.":4.544,"RT..min.":161.9964,"First.Scan":82774,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_03.raw","File.ID":"F2","Abundance..126":135794.19482,"Abundance..127N":118574.84759,"Abundance..127C":120149.85338,"Abundance..128N":128761.66512,"Abundance..128C":131507.33868,"Abundance..129N":132073.14587,"Abundance..129C":117237.93092,"Abundance..130N":134100.77439,"Abundance..130C":113697.38008,"Abundance..131":131002.12786,"Ions.Score":36,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.00247,"Percolator.q.Value":0.00012,"Percolator.PEP":0.0008}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (N2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].sIEAVHEDIr.[V]","Modifications":"N-Term(TMT6plex); R10(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P23919","Master.Protein.Descriptions":"Thymidylate kinase OS=Homo sapiens GN=DTYMK PE=1 SV=4","Protein.Accessions":"P23919","Protein.Descriptions":"Thymidylate kinase OS=Homo sapiens GN=DTYMK PE=1 SV=4","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.6818,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":469.92697,"MH...Da.":1407.76636,"Theo..MH...Da.":1407.76688,"DeltaM..ppm.":-0.37,"Deltam.z..Da.":-0.00017,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":32.27546,"Average.Reporter.S.N":41.2,"Ion.Inject.Time..ms.":5.346,"RT..min.":70.0828,"First.Scan":30734,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_03.raw","File.ID":"F2","Abundance..126":133484.54278,"Abundance..127N":131542.06425,"Abundance..127C":115136.14132,"Abundance..128N":126293.63246,"Abundance..128C":138376.11598,"Abundance..129N":114510.64128,"Abundance..129C":142282.6439,"Abundance..130N":127022.1968,"Abundance..130C":122402.01733,"Abundance..131":140377.34835,"Ions.Score":22,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":0.04017,"Percolator.q.Value":0.00012,"Percolator.PEP":0.00085}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (N2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].gSITSVQAIYVPADDLTDPAPATTFAHLDATTVLSr.[A]","Modifications":"N-Term(TMT6plex); R36(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":4,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":989.27173,"MH...Da.":3954.06508,"Theo..MH...Da.":3954.05731,"DeltaM..ppm.":1.97,"Deltam.z..Da.":0.00194,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":13.52858,"Average.Reporter.S.N":8.7,"Ion.Inject.Time..ms.":22.746,"RT..min.":190.4207,"First.Scan":98875,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_03.raw","File.ID":"F2","Abundance..126":7103.89756,"Abundance..127N":10801.92851,"Abundance..127C":8613.32884,"Abundance..128N":6278.92726,"Abundance..128C":13547.73883,"Abundance..129N":9999.91291,"Abundance..129C":8036.4857,"Abundance..130N":9266.08771,"Abundance..130C":14101.45703,"Abundance..131":9290.02991,"Ions.Score":9,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":0.98739,"Percolator.q.Value":0.00012,"Percolator.PEP":0.00074}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (F2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].cSEGSFLLTTFPrPVTVEPMDQLDDEEGLPEk.[L]","Modifications":"N-Term(TMT6plex); C1(Carbamidomethyl); R13(Label:13C(6)15N(4)); K32(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q15233","Master.Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","Protein.Accessions":"Q15233","Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","X..Missed.Cleavages":1,"Charge":4,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":1029.02258,"MH...Da.":4113.0685,"Theo..MH...Da.":4113.05674,"DeltaM..ppm.":2.86,"Deltam.z..Da.":0.00294,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":0,"Average.Reporter.S.N":14.1,"Ion.Inject.Time..ms.":12.167,"RT..min.":187.3356,"First.Scan":98193,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture4_02.raw","File.ID":"F10","Abundance..126":24887.32657,"Abundance..127N":20511.84801,"Abundance..127C":19496.35299,"Abundance..128N":18891.91737,"Abundance..128C":26115.94007,"Abundance..129N":17622.67699,"Abundance..129C":24077.14431,"Abundance..130N":26185.47193,"Abundance..130C":19304.73568,"Abundance..131":22439.20459,"Ions.Score":18,"Identity.Strict":26,"Identity.Relaxed":19,"Expectation.Value":0.07477,"Percolator.q.Value":0.00012,"Percolator.PEP":0.00095}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (G2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].vLDSGAPIk.[I]","Modifications":"N-Term(TMT6plex); K9(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":0.7105,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":683.43414,"MH...Da.":1365.86101,"Theo..MH...Da.":1365.85972,"DeltaM..ppm.":0.94,"Deltam.z..Da.":0.00064,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":16.31613,"Average.Reporter.S.N":97.5,"Ion.Inject.Time..ms.":1.604,"RT..min.":116.4991,"First.Scan":55610,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_02.raw","File.ID":"F9","Abundance..126":1092859.09917,"Abundance..127N":1009565.88806,"Abundance..127C":1024565.3018,"Abundance..128N":1039006.27336,"Abundance..128C":921993.50483,"Abundance..129N":962580.00107,"Abundance..129C":1014695.75438,"Abundance..130N":958408.93278,"Abundance..130C":1094406.76649,"Abundance..131":1111043.62968,"Ions.Score":38,"Identity.Strict":27,"Identity.Relaxed":20,"Expectation.Value":0.0009,"Percolator.q.Value":0.00013,"Percolator.PEP":0.00053}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (G2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].iMNVIGEPIDEr.[G]","Modifications":"N-Term(TMT6plex); R12(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":812.94489,"MH...Da.":1624.88249,"Theo..MH...Da.":1624.88053,"DeltaM..ppm.":1.21,"Deltam.z..Da.":0.00098,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":7.71106,"Average.Reporter.S.N":22.8,"Ion.Inject.Time..ms.":5.513,"RT..min.":154.7968,"First.Scan":77088,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_02.raw","File.ID":"F9","Abundance..126":140647.69233,"Abundance..127N":144513.22809,"Abundance..127C":164390.62968,"Abundance..128N":130654.35885,"Abundance..128C":129431.90056,"Abundance..129N":125952.47967,"Abundance..129C":147078.88825,"Abundance..130N":159480.24003,"Abundance..130C":117848.96107,"Abundance..131":117989.03301,"Ions.Score":25,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.02854,"Percolator.q.Value":0.00013,"Percolator.PEP":0.00066}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (G2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].fAQPGSFEYEYAMr.[W]","Modifications":"N-Term(TMT6plex); R14(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q15233","Master.Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","Protein.Accessions":"Q15233","Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":967.96368,"MH...Da.":1934.92009,"Theo..MH...Da.":1934.91837,"DeltaM..ppm.":0.89,"Deltam.z..Da.":0.00086,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":0,"Average.Reporter.S.N":24.2,"Ion.Inject.Time..ms.":27.691,"RT..min.":154.3705,"First.Scan":76841,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_02.raw","File.ID":"F9","Abundance..126":29991.47438,"Abundance..127N":32684.67441,"Abundance..127C":27736.07966,"Abundance..128N":39408.75409,"Abundance..128C":30092.37674,"Abundance..129N":26511.48288,"Abundance..129C":36007.22632,"Abundance..130N":26388.75295,"Abundance..130C":29991.53994,"Abundance..131":34408.80552,"Ions.Score":26,"Identity.Strict":22,"Identity.Relaxed":15,"Expectation.Value":0.00437,"Percolator.q.Value":0.00013,"Percolator.PEP":0.00066}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (M2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].tTLLPGLLk.[T]","Modifications":"N-Term(TMT6plex); K9(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q9NSD9","Master.Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","Protein.Accessions":"Q9NSD9","Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.6842,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":474.65775,"MH...Da.":1421.95868,"Theo..MH...Da.":1421.95871,"DeltaM..ppm.":-0.02,"Deltam.z..Da.":-1e-05,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":0,"Average.Reporter.S.N":52.4,"Ion.Inject.Time..ms.":22.988,"RT..min.":164.6049,"First.Scan":82867,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_03.raw","File.ID":"F3","Abundance..126":21657.93806,"Abundance..127N":22441.23881,"Abundance..127C":20320.55024,"Abundance..128N":19930.85584,"Abundance..128C":25423.94174,"Abundance..129N":21134.75415,"Abundance..129C":24439.87599,"Abundance..130N":23054.08105,"Abundance..130C":22253.11204,"Abundance..131":25743.11429,"Ions.Score":38,"Identity.Strict":21,"Identity.Relaxed":14,"Expectation.Value":0.00023,"Percolator.q.Value":0.00013,"Percolator.PEP":0.00045}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (I2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].vIHDNFGIVEGLMTTVHAITATQk.[T]","Modifications":"N-Term(TMT6plex); K24(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":0,"Charge":5,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":613.14624,"MH...Da.":3061.70209,"Theo..MH...Da.":3061.7,"DeltaM..ppm.":0.69,"Deltam.z..Da.":0.00042,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":20.76741,"Average.Reporter.S.N":21.2,"Ion.Inject.Time..ms.":21.272,"RT..min.":199.174,"First.Scan":108712,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_01.raw","File.ID":"F7","Abundance..126":27652.6023,"Abundance..127N":28917.41179,"Abundance..127C":29026.34496,"Abundance..128N":26076.85028,"Abundance..128C":20475.84299,"Abundance..129N":22989.81727,"Abundance..129C":27190.64937,"Abundance..130N":25820.24698,"Abundance..130C":32180.34715,"Abundance..131":24527.81759,"Ions.Score":2,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":6.52353,"Percolator.q.Value":0.00014,"Percolator.PEP":0.00062}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (C2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].vVDLMAHmASk.[E]","Modifications":"N-Term(TMT6plex); M8(Oxidation); K11(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":561.98517,"MH...Da.":1683.94095,"Theo..MH...Da.":1683.94175,"DeltaM..ppm.":-0.48,"Deltam.z..Da.":-0.00027,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":34.25019,"Average.Reporter.S.N":49.4,"Ion.Inject.Time..ms.":7.583,"RT..min.":110.6484,"First.Scan":54282,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture4_01.raw","File.ID":"F13","Abundance..126":82378.46658,"Abundance..127N":93018.69375,"Abundance..127C":90805.22297,"Abundance..128N":111797.30093,"Abundance..128C":102426.46221,"Abundance..129N":101253.47768,"Abundance..129C":102167.7028,"Abundance..130N":118296.22678,"Abundance..130C":101243.24215,"Abundance..131":97051.65283,"Ions.Score":17,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.18028,"Percolator.q.Value":0.00015,"Percolator.PEP":0.0004}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (C2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].aDIIHAcDIVEDAAIAYGYNNIQMTLPk.[T]","Modifications":"N-Term(TMT6plex); C7(Carbamidomethyl); K28(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q9NSD9","Master.Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","Protein.Accessions":"Q9NSD9","Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","X..Missed.Cleavages":0,"Charge":4,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":897.22009,"MH...Da.":3585.85854,"Theo..MH...Da.":3585.8577,"DeltaM..ppm.":0.24,"Deltam.z..Da.":0.00021,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":2.44941,"Average.Reporter.S.N":10.5,"Ion.Inject.Time..ms.":8.366,"RT..min.":206.1687,"First.Scan":107432,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture4_01.raw","File.ID":"F13","Abundance..126":9081.08742,"Abundance..127N":17336.64367,"Abundance..127C":12133.60206,"Abundance..128N":14297.10773,"Abundance..128C":7082.16596,"Abundance..129N":11491.40306,"Abundance..129C":14110.89509,"Abundance..130N":18432.28417,"Abundance..130C":15258.13385,"Abundance..131":14952.60441,"Ions.Score":22,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":0.04969,"Percolator.q.Value":0.00015,"Percolator.PEP":0.00047}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (C2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].wGDAGAEYVVESTGVFTTMEk.[A]","Modifications":"N-Term(TMT6plex); K21(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":915.13794,"MH...Da.":2743.39927,"Theo..MH...Da.":2743.37805,"DeltaM..ppm.":7.73,"Deltam.z..Da.":0.00707,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":5.87422,"Average.Reporter.S.N":11.1,"Ion.Inject.Time..ms.":50,"RT..min.":254.51,"First.Scan":129742,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture4_01.raw","File.ID":"F13","Abundance..126":3061.89836,"Abundance..127N":3198.88305,"Abundance..127C":2366.37409,"Abundance..128N":4337.81438,"Abundance..128C":2961.6019,"Abundance..129N":4823.08666,"Abundance..129C":4273.78739,"Abundance..130N":2986.61114,"Abundance..130C":4497.07993,"Abundance..131":4795.9888,"Ions.Score":31,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":0.00511,"Percolator.q.Value":0.00015,"Percolator.PEP":0.00046}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (C2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].yDLLcLEGLVr.[G]","Modifications":"N-Term(TMT6plex); C5(Carbamidomethyl); R11(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q9NSD9","Master.Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","Protein.Accessions":"Q9NSD9","Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":530.63116,"MH...Da.":1589.87894,"Theo..MH...Da.":1589.8798,"DeltaM..ppm.":-0.54,"Deltam.z..Da.":-0.00029,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":64.72268,"Average.Reporter.S.N":48.9,"Ion.Inject.Time..ms.":50,"RT..min.":187.5411,"First.Scan":97675,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture4_01.raw","File.ID":"F13","Abundance..126":15764.6678,"Abundance..127N":18232.35968,"Abundance..127C":14176.65667,"Abundance..128N":16509.1286,"Abundance..128C":18946.70028,"Abundance..129N":17057.92185,"Abundance..129C":16550.29843,"Abundance..130N":15896.38384,"Abundance..130C":16335.96471,"Abundance..131":17451.15605,"Ions.Score":9,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.97376,"Percolator.q.Value":0.00015,"Percolator.PEP":0.00047}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (K2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].iISNAScTTNcLAPLAk.[V]","Modifications":"N-Term(TMT6plex); C7(Carbamidomethyl); C11(Carbamidomethyl); K17(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":767.42645,"MH...Da.":2300.2648,"Theo..MH...Da.":2300.25979,"DeltaM..ppm.":2.18,"Deltam.z..Da.":0.00167,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":3.46668,"Average.Reporter.S.N":26,"Ion.Inject.Time..ms.":23.024,"RT..min.":149.17,"First.Scan":78156,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_03.raw","File.ID":"F5","Abundance..126":1315596.35944,"Abundance..127N":1352078.80787,"Abundance..127C":1486174.21068,"Abundance..128N":1395024.62799,"Abundance..128C":1561742.78676,"Abundance..129N":1477793.2494,"Abundance..129C":1526593.7793,"Abundance..130N":1872370.12936,"Abundance..130C":2242090.08417,"Abundance..131":1676772.76385,"Ions.Score":6,"Identity.Strict":30,"Identity.Relaxed":23,"Expectation.Value":2.753,"Percolator.q.Value":0.00015,"Percolator.PEP":0.00026}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (H2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[K].rVIISAPSADAPMFVmGVNHEk.[Y]","Modifications":"N-Term(TMT6plex); R1(Label:13C(6)15N(4)); M16(Oxidation); K22(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":1,"Charge":4,"DeltaScore":0.5,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":716.14563,"MH...Da.":2861.56069,"Theo..MH...Da.":2861.55368,"DeltaM..ppm.":2.45,"Deltam.z..Da.":0.00175,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":24.99224,"Average.Reporter.S.N":41,"Ion.Inject.Time..ms.":8.78,"RT..min.":143.1419,"First.Scan":70874,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_02.raw","File.ID":"F8","Abundance..126":54823.90597,"Abundance..127N":69852.02115,"Abundance..127C":83154.99925,"Abundance..128N":56666.8035,"Abundance..128C":71253.47545,"Abundance..129N":59976.37151,"Abundance..129C":79805.45322,"Abundance..130N":65600.00956,"Abundance..130C":76112.39725,"Abundance..131":67546.06511,"Ions.Score":8,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":1.63784,"Percolator.q.Value":0.00016,"Percolator.PEP":0.00122}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (M2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].eVLGDAVPDEILIEAVLk.[N]","Modifications":"N-Term(TMT6plex); K18(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q9Y450","Master.Protein.Descriptions":"HBS1-like protein OS=Homo sapiens GN=HBS1L PE=1 SV=1","Protein.Accessions":"Q9Y450","Protein.Descriptions":"HBS1-like protein OS=Homo sapiens GN=HBS1L PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.8571,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":797.13995,"MH...Da.":2389.40531,"Theo..MH...Da.":2389.40855,"DeltaM..ppm.":-1.36,"Deltam.z..Da.":-0.00108,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":48.2281,"Average.Reporter.S.N":15,"Ion.Inject.Time..ms.":50,"RT..min.":218.5102,"First.Scan":111437,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_03.raw","File.ID":"F3","Abundance..126":4753.85376,"Abundance..127N":3393.60348,"Abundance..127C":4501.4285,"Abundance..128N":4912.67764,"Abundance..128C":6459.29801,"Abundance..129N":5433.66199,"Abundance..129C":6432.77597,"Abundance..130N":4537.91722,"Abundance..130C":4900.5997,"Abundance..131":5734.43208,"Ions.Score":21,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":0.05987,"Percolator.q.Value":0.00016,"Percolator.PEP":0.00073}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (M2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].gIVEFSGkPAAr.[K]","Modifications":"N-Term(TMT6plex); K8(Label); R12(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q15233","Master.Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","Protein.Accessions":"Q15233","Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","X..Missed.Cleavages":1,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":570.01434,"MH...Da.":1708.02848,"Theo..MH...Da.":1708.02768,"DeltaM..ppm.":0.47,"Deltam.z..Da.":0.00027,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":9.03589,"Average.Reporter.S.N":47.4,"Ion.Inject.Time..ms.":6.155,"RT..min.":98.7579,"First.Scan":45745,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_03.raw","File.ID":"F3","Abundance..126":90144.04587,"Abundance..127N":75176.3144,"Abundance..127C":89185.12451,"Abundance..128N":88957.30776,"Abundance..128C":79887.34516,"Abundance..129N":77715.17078,"Abundance..129C":85924.82497,"Abundance..130N":85689.49185,"Abundance..130C":75295.02231,"Abundance..131":72482.04991,"Ions.Score":30,"Identity.Strict":30,"Identity.Relaxed":23,"Expectation.Value":0.01136,"Percolator.q.Value":0.00016,"Percolator.PEP":0.00093}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (G2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].gAMPPAPVPAGTPAPPGPATMMPDGTLGLTPPTTEr.[F]","Modifications":"N-Term(TMT6plex); R36(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q15233","Master.Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","Protein.Accessions":"Q15233","Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","X..Missed.Cleavages":0,"Charge":4,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":923.47644,"MH...Da.":3690.88393,"Theo..MH...Da.":3690.87704,"DeltaM..ppm.":1.87,"Deltam.z..Da.":0.00172,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":17.08615,"Average.Reporter.S.N":11.5,"Ion.Inject.Time..ms.":18.411,"RT..min.":182.2828,"First.Scan":92690,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_02.raw","File.ID":"F9","Abundance..126":8969.664,"Abundance..127N":15009.43056,"Abundance..127C":12195.70725,"Abundance..128N":11634.06282,"Abundance..128C":10132.90585,"Abundance..129N":12352.73805,"Abundance..129C":11994.91663,"Abundance..130N":10784.78933,"Abundance..130C":15179.32413,"Abundance..131":12125.27576,"Ions.Score":25,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":0.0229,"Percolator.q.Value":0.00017,"Percolator.PEP":0.00066}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (L2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].gFQQILAGEYDHLPEQAFYmVGPIEEAVAk.[A]","Modifications":"N-Term(TMT6plex); M20(Oxidation); K30(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":4,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":959.00055,"MH...Da.":3832.98037,"Theo..MH...Da.":3832.97517,"DeltaM..ppm.":1.36,"Deltam.z..Da.":0.0013,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":37.43557,"Average.Reporter.S.N":20.3,"Ion.Inject.Time..ms.":5.437,"RT..min.":196.1003,"First.Scan":100769,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_02.raw","File.ID":"F4","Abundance..126":84894.69986,"Abundance..127N":62849.66927,"Abundance..127C":77020.12053,"Abundance..128N":85872.5184,"Abundance..128C":69722.67296,"Abundance..129N":81070.87822,"Abundance..129C":84208.94079,"Abundance..130N":71344.99153,"Abundance..130C":72481.65279,"Abundance..131":72949.72123,"Ions.Score":10,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":0.76973,"Percolator.q.Value":0.00021,"Percolator.PEP":0.00196}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (E2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].cSEGSFLLTTFPr.[P]","Modifications":"N-Term(TMT6plex); C1(Carbamidomethyl); R13(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q15233","Master.Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","Protein.Accessions":"Q15233","Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":585.30615,"MH...Da.":1753.9039,"Theo..MH...Da.":1753.90199,"DeltaM..ppm.":1.09,"Deltam.z..Da.":0.00064,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":33.91448,"Average.Reporter.S.N":29.9,"Ion.Inject.Time..ms.":26.427,"RT..min.":162.3477,"First.Scan":80821,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_01.raw","File.ID":"F11","Abundance..126":34668.35315,"Abundance..127N":37303.92628,"Abundance..127C":40223.77788,"Abundance..128N":34294.01188,"Abundance..128C":35431.45241,"Abundance..129N":35880.50126,"Abundance..129C":36682.131,"Abundance..130N":31859.08664,"Abundance..130C":36519.74957,"Abundance..131":40762.88876,"Ions.Score":17,"Identity.Strict":26,"Identity.Relaxed":19,"Expectation.Value":0.08699,"Percolator.q.Value":0.00021,"Percolator.PEP":0.002}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (O2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].qFAPIHAEAPEFMEMSVEQEILVTGIk.[V]","Modifications":"N-Term(TMT6plex); K27(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":4,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":878.46838,"MH...Da.":3510.85171,"Theo..MH...Da.":3510.85082,"DeltaM..ppm.":0.25,"Deltam.z..Da.":0.00022,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":5.53673,"Average.Reporter.S.N":7.7,"Ion.Inject.Time..ms.":1.158,"RT..min.":195.8262,"First.Scan":103903,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_03.raw","File.ID":"F1","Abundance..126":23506.70147,"Abundance..127N":25855.78528,"Abundance..127C":48997.11606,"Abundance..128N":48104.09091,"Abundance..128C":41865.12745,"Abundance..129N":39055.97537,"Abundance..129C":27675.18955,"Abundance..130N":31501.39604,"Abundance..130C":49823.50935,"Abundance..131":42221.2349,"Ions.Score":19,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.10927,"Percolator.q.Value":0.00022,"Percolator.PEP":0.00095}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (K2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].gAMPPAPVPAGTPAPPGPATmMPDGTLGLTPPTTEr.[F]","Modifications":"N-Term(TMT6plex); M21(Oxidation); R36(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q15233","Master.Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","Protein.Accessions":"Q15233","Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","X..Missed.Cleavages":0,"Charge":4,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":2,"m.z..Da.":927.48035,"MH...Da.":3706.89956,"Theo..MH...Da.":3706.87196,"DeltaM..ppm.":7.44,"Deltam.z..Da.":0.0069,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":0,"Average.Reporter.S.N":6.3,"Ion.Inject.Time..ms.":31.349,"RT..min.":156.6712,"First.Scan":82612,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_03.raw","File.ID":"F5","Abundance..126":4475.42597,"Abundance..127N":6166.04015,"Abundance..127C":7236.97313,"Abundance..128N":6210.92868,"Abundance..128C":6818.5604,"Abundance..129N":6086.15106,"Abundance..129C":6321.94686,"Abundance..130N":7944.54109,"Abundance..130C":7627.1895,"Abundance..131":3102.29685,"Ions.Score":3,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":3.04301,"Percolator.q.Value":0.00023,"Percolator.PEP":0.00081}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (N2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].qFAPIHAEAPEFMEMSVEQEILVTGIk.[V]","Modifications":"N-Term(TMT6plex); K27(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":4,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":878.4682,"MH...Da.":3510.85097,"Theo..MH...Da.":3510.85082,"DeltaM..ppm.":0.04,"Deltam.z..Da.":0.00004,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":0,"Average.Reporter.S.N":1.3,"Ion.Inject.Time..ms.":1.144,"RT..min.":196.0795,"First.Scan":101941,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_03.raw","File.ID":"F2","Abundance..128N":32957.37891,"Abundance..128C":48535.03906,"Abundance..130N":47810.31003,"Abundance..130C":22258.63867,"Abundance..131":42861.13307,"Ions.Score":16,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.21184,"Percolator.q.Value":0.00027,"Percolator.PEP":0.00141}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (O2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].lGVLHPDVITk.[F]","Modifications":"N-Term(TMT6plex); K11(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q9NSD9","Master.Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","Protein.Accessions":"Q9NSD9","Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":553.35547,"MH...Da.":1658.05185,"Theo..MH...Da.":1658.04965,"DeltaM..ppm.":1.33,"Deltam.z..Da.":0.00074,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":8.56448,"Average.Reporter.S.N":40.6,"Ion.Inject.Time..ms.":3.977,"RT..min.":128.3317,"First.Scan":64963,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_03.raw","File.ID":"F1","Abundance..126":98589.15965,"Abundance..127N":95518.05727,"Abundance..127C":108604.45001,"Abundance..128N":80396.78787,"Abundance..128C":104894.36852,"Abundance..129N":88349.87192,"Abundance..129C":101206.87208,"Abundance..130N":115272.83842,"Abundance..130C":112775.12624,"Abundance..131":117511.50697,"Ions.Score":36,"Identity.Strict":27,"Identity.Relaxed":20,"Expectation.Value":0.00147,"Percolator.q.Value":0.00027,"Percolator.PEP":0.0015}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (O2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[R].yAFSGVAFTGAk.[E]","Modifications":"N-Term(TMT6plex); K12(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P23919","Master.Protein.Descriptions":"Thymidylate kinase OS=Homo sapiens GN=DTYMK PE=1 SV=4","Protein.Accessions":"P23919","Protein.Descriptions":"Thymidylate kinase OS=Homo sapiens GN=DTYMK PE=1 SV=4","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.5833,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":562.32379,"MH...Da.":1684.95682,"Theo..MH...Da.":1684.95541,"DeltaM..ppm.":0.84,"Deltam.z..Da.":0.00047,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":20.75288,"Average.Reporter.S.N":39.3,"Ion.Inject.Time..ms.":5.661,"RT..min.":152.2944,"First.Scan":78856,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_03.raw","File.ID":"F1","Abundance..126":187276.51576,"Abundance..127N":158781.12682,"Abundance..127C":179222.6705,"Abundance..128N":182152.85096,"Abundance..128C":179863.42282,"Abundance..129N":154649.57311,"Abundance..129C":172806.01877,"Abundance..130N":165604.07804,"Abundance..130C":171043.2278,"Abundance..131":179795.42704,"Ions.Score":12,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.58028,"Percolator.q.Value":0.00027,"Percolator.PEP":0.0018}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (O2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[K].rVIISAPSADAPMFVMGVNHEk.[Y]","Modifications":"N-Term(TMT6plex); R1(Label:13C(6)15N(4)); K22(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":1,"Charge":5,"DeltaScore":0.9565,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":569.91821,"MH...Da.":2845.56196,"Theo..MH...Da.":2845.55876,"DeltaM..ppm.":1.12,"Deltam.z..Da.":0.00064,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":0.68733,"Average.Reporter.S.N":15.5,"Ion.Inject.Time..ms.":0.183,"RT..min.":143.9944,"First.Scan":74026,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_03.raw","File.ID":"F1","Abundance..126":313316.9994,"Abundance..127N":366897.11526,"Abundance..127C":305762.69594,"Abundance..128N":386123.82226,"Abundance..128C":416095.63908,"Abundance..129N":415075.59373,"Abundance..129C":340681.76535,"Abundance..130N":345036.53165,"Abundance..130C":326903.80628,"Abundance..131":417827.44599,"Ions.Score":23,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.04007,"Percolator.q.Value":0.00027,"Percolator.PEP":0.00164}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (J2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[R].hEHQVMLmr.[Q]","Modifications":"N-Term(TMT6plex); M8(Oxidation); R9(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q15233","Master.Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","Protein.Accessions":"Q15233","Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","X..Missed.Cleavages":0,"Charge":4,"DeltaScore":0.5455,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":359.68961,"MH...Da.":1435.73659,"Theo..MH...Da.":1435.73751,"DeltaM..ppm.":-0.64,"Deltam.z..Da.":-0.00023,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":58.27245,"Average.Reporter.S.N":15.3,"Ion.Inject.Time..ms.":50,"RT..min.":30.0629,"First.Scan":10216,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture4_03.raw","File.ID":"F6","Abundance..126":4441.40816,"Abundance..127N":6742.66059,"Abundance..127C":5281.15519,"Abundance..128N":7871.65751,"Abundance..128C":5481.47418,"Abundance..129N":4677.85194,"Abundance..129C":4529.64115,"Abundance..130N":5401.81164,"Abundance..130C":4080.01021,"Abundance..131":6276.18252,"Ions.Score":11,"Identity.Strict":25,"Identity.Relaxed":18,"Expectation.Value":0.31584,"Percolator.q.Value":0.00027,"Percolator.PEP":0.00175}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (K2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].vIISAPSADAPmFVMGVNHEk.[Y]","Modifications":"N-Term(TMT6plex); M12(Oxidation); K21(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":0,"Charge":4,"DeltaScore":0.0833,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":674.61676,"MH...Da.":2695.44521,"Theo..MH...Da.":2695.4443,"DeltaM..ppm.":0.34,"Deltam.z..Da.":0.00023,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":27.41115,"Average.Reporter.S.N":21.6,"Ion.Inject.Time..ms.":6.135,"RT..min.":161.5686,"First.Scan":85514,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_03.raw","File.ID":"F5","Abundance..126":50677.9448,"Abundance..127N":43320.01809,"Abundance..127C":44138.1428,"Abundance..128N":41013.75143,"Abundance..128C":43690.77174,"Abundance..129N":43268.57067,"Abundance..129C":52198.91134,"Abundance..130N":43129.26331,"Abundance..130C":50496.13959,"Abundance..131":50957.68212,"Ions.Score":12,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.53188,"Percolator.q.Value":0.00027,"Percolator.PEP":0.00118}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (K2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].qQQDQVDr.[N]","Modifications":"N-Term(TMT6plex); R8(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q15233","Master.Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","Protein.Accessions":"Q15233","Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":0.64,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":628.32727,"MH...Da.":1255.64726,"Theo..MH...Da.":1255.64676,"DeltaM..ppm.":0.4,"Deltam.z..Da.":0.00025,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":22.55323,"Average.Reporter.S.N":4,"Ion.Inject.Time..ms.":50,"RT..min.":17.8377,"First.Scan":4752,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_03.raw","File.ID":"F5","Abundance..126":863.02398,"Abundance..127N":1590.71407,"Abundance..127C":1046.92516,"Abundance..128N":1318.81734,"Abundance..128C":360.41073,"Abundance..129N":2078.6677,"Abundance..129C":1377.69436,"Abundance..130N":1010.85303,"Abundance..130C":2495.42628,"Abundance..131":1605.83522,"Ions.Score":25,"Identity.Strict":25,"Identity.Relaxed":18,"Expectation.Value":0.00906,"Percolator.q.Value":0.00027,"Percolator.PEP":0.00144}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (K2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].fAQPGSFEYEYAMr.[W]","Modifications":"N-Term(TMT6plex); R14(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q15233","Master.Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","Protein.Accessions":"Q15233","Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":967.96356,"MH...Da.":1934.91985,"Theo..MH...Da.":1934.91837,"DeltaM..ppm.":0.76,"Deltam.z..Da.":0.00074,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":0,"Average.Reporter.S.N":41.6,"Ion.Inject.Time..ms.":15.747,"RT..min.":154.0874,"First.Scan":81071,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_03.raw","File.ID":"F5","Abundance..126":31972.81942,"Abundance..127N":28749.83142,"Abundance..127C":32934.59341,"Abundance..128N":36665.3126,"Abundance..128C":29732.22614,"Abundance..129N":32178.4157,"Abundance..129C":36550.63189,"Abundance..130N":29332.61641,"Abundance..130C":32401.95544,"Abundance..131":29558.19431,"Ions.Score":23,"Identity.Strict":22,"Identity.Relaxed":15,"Expectation.Value":0.00743,"Percolator.q.Value":0.00027,"Percolator.PEP":0.00138}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (K2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].dTEEVELk.[F]","Modifications":"N-Term(TMT6plex); K8(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q9UGP8","Master.Protein.Descriptions":"Translocation protein SEC63 homolog OS=Homo sapiens GN=SEC63 PE=1 SV=2","Protein.Accessions":"Q9UGP8","Protein.Descriptions":"Translocation protein SEC63 homolog OS=Homo sapiens GN=SEC63 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":0.7907,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":714.90631,"MH...Da.":1428.80535,"Theo..MH...Da.":1428.80775,"DeltaM..ppm.":-1.68,"Deltam.z..Da.":-0.0012,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":0,"Average.Reporter.S.N":15.4,"Ion.Inject.Time..ms.":4.167,"RT..min.":99,"First.Scan":48648,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_03.raw","File.ID":"F5","Abundance..126":33740.7426,"Abundance..127N":35258.30491,"Abundance..127C":30570.60941,"Abundance..128N":40064.9216,"Abundance..128C":43196.94513,"Abundance..129N":29425.53537,"Abundance..129C":41398.74929,"Abundance..130N":40281.58133,"Abundance..130C":41653.8934,"Abundance..131":44121.52754,"Ions.Score":43,"Identity.Strict":30,"Identity.Relaxed":23,"Expectation.Value":0.0005,"Percolator.q.Value":0.00027,"Percolator.PEP":0.00122}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (K2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].gFQQILAGEYDHLPEQAFYmVGPIEEAVAk.[A]","Modifications":"N-Term(TMT6plex); M20(Oxidation); K30(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":4,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":958.99963,"MH...Da.":3832.97671,"Theo..MH...Da.":3832.97517,"DeltaM..ppm.":0.4,"Deltam.z..Da.":0.00038,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":79.23206,"Average.Reporter.S.N":12.2,"Ion.Inject.Time..ms.":25.011,"RT..min.":210.1117,"First.Scan":113165,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_03.raw","File.ID":"F5","Abundance..126":5797.57519,"Abundance..127N":5489.84335,"Abundance..127C":6667.06026,"Abundance..128N":7856.2707,"Abundance..128C":9310.48146,"Abundance..129N":7661.55991,"Abundance..129C":8135.16279,"Abundance..130N":8271.82459,"Abundance..130C":8132.12431,"Abundance..131":8815.33092,"Ions.Score":4,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":2.63018,"Percolator.q.Value":0.00027,"Percolator.PEP":0.0015}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (K2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[K].lFEISDIVIk.[D]","Modifications":"N-Term(TMT6plex); K10(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q9NSD9","Master.Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","Protein.Accessions":"Q9NSD9","Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":0.7857,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":822.01794,"MH...Da.":1643.02861,"Theo..MH...Da.":1643.02752,"DeltaM..ppm.":0.67,"Deltam.z..Da.":0.00055,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":27.32206,"Average.Reporter.S.N":9.2,"Ion.Inject.Time..ms.":2.469,"RT..min.":190.4537,"First.Scan":102377,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_03.raw","File.ID":"F5","Abundance..126":47742.89689,"Abundance..127N":49507.6281,"Abundance..127C":56109.03979,"Abundance..128N":77011.72808,"Abundance..128C":81161.7336,"Abundance..129N":61703.27029,"Abundance..129C":87926.29937,"Abundance..130N":55539.14597,"Abundance..130C":75514.31453,"Abundance..131":78554.80359,"Ions.Score":56,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":0.00002,"Percolator.q.Value":0.00027,"Percolator.PEP":0.00152}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (K2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].lGFSEVELVQMVVDGVk.[L]","Modifications":"N-Term(TMT6plex); K17(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P12277","Master.Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","Protein.Accessions":"P12277","Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":772.44421,"MH...Da.":2315.31809,"Theo..MH...Da.":2315.31763,"DeltaM..ppm.":0.2,"Deltam.z..Da.":0.00015,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":49.15933,"Average.Reporter.S.N":12.4,"Ion.Inject.Time..ms.":25.508,"RT..min.":205.9635,"First.Scan":110957,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_03.raw","File.ID":"F5","Abundance..126":5310.76061,"Abundance..127N":3934.66173,"Abundance..127C":5396.32235,"Abundance..128N":5311.21066,"Abundance..128C":4666.13839,"Abundance..129N":7213.20166,"Abundance..129C":8025.57478,"Abundance..130N":5936.47708,"Abundance..130C":4944.30194,"Abundance..131":4572.99095,"Ions.Score":18,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.13063,"Percolator.q.Value":0.00027,"Percolator.PEP":0.00154}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (A2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[R].eGNDLYHEMIESGVINLk.[D]","Modifications":"N-Term(TMT6plex); K18(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":4,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":632.59039,"MH...Da.":2527.33974,"Theo..MH...Da.":2527.33579,"DeltaM..ppm.":1.56,"Deltam.z..Da.":0.00099,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":32.48758,"Average.Reporter.S.N":18.5,"Ion.Inject.Time..ms.":3.514,"RT..min.":174.7155,"First.Scan":89670,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_01.raw","File.ID":"F15","Abundance..126":75736.5954,"Abundance..127N":59138.3343,"Abundance..127C":54453.94628,"Abundance..128N":53035.78663,"Abundance..128C":71269.37014,"Abundance..129N":69764.13107,"Abundance..129C":58298.46339,"Abundance..130N":69816.64509,"Abundance..130C":58716.30296,"Abundance..131":63113.87934,"Ions.Score":6,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":1.92613,"Percolator.q.Value":0.00027,"Percolator.PEP":0.00222}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (A2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[K].sIEAVHEDIr.[V]","Modifications":"N-Term(TMT6plex); R10(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P23919","Master.Protein.Descriptions":"Thymidylate kinase OS=Homo sapiens GN=DTYMK PE=1 SV=4","Protein.Accessions":"P23919","Protein.Descriptions":"Thymidylate kinase OS=Homo sapiens GN=DTYMK PE=1 SV=4","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.55,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":469.92712,"MH...Da.":1407.76682,"Theo..MH...Da.":1407.76688,"DeltaM..ppm.":-0.04,"Deltam.z..Da.":-0.00002,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":15.03931,"Average.Reporter.S.N":46.9,"Ion.Inject.Time..ms.":4.581,"RT..min.":71.437,"First.Scan":31004,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_01.raw","File.ID":"F15","Abundance..126":86097.26681,"Abundance..127N":112074.01821,"Abundance..127C":139851.03877,"Abundance..128N":122041.75013,"Abundance..128C":110461.56451,"Abundance..129N":119759.40143,"Abundance..129C":126949.69598,"Abundance..130N":128635.29582,"Abundance..130C":138021.50332,"Abundance..131":116687.12341,"Ions.Score":20,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":0.0665,"Percolator.q.Value":0.00027,"Percolator.PEP":0.0027}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (J2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].sIEAVHEDIr.[V]","Modifications":"N-Term(TMT6plex); R10(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P23919","Master.Protein.Descriptions":"Thymidylate kinase OS=Homo sapiens GN=DTYMK PE=1 SV=4","Protein.Accessions":"P23919","Protein.Descriptions":"Thymidylate kinase OS=Homo sapiens GN=DTYMK PE=1 SV=4","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.6154,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":469.92712,"MH...Da.":1407.76682,"Theo..MH...Da.":1407.76688,"DeltaM..ppm.":-0.04,"Deltam.z..Da.":-0.00002,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":20.41865,"Average.Reporter.S.N":66.3,"Ion.Inject.Time..ms.":4.183,"RT..min.":70.5847,"First.Scan":31732,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture4_03.raw","File.ID":"F6","Abundance..126":189316.79255,"Abundance..127N":184983.91355,"Abundance..127C":159640.7498,"Abundance..128N":200366.21138,"Abundance..128C":198274.9695,"Abundance..129N":187111.0519,"Abundance..129C":175599.00359,"Abundance..130N":176982.34413,"Abundance..130C":176041.11411,"Abundance..131":176838.53248,"Ions.Score":26,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":0.01765,"Percolator.q.Value":0.0003,"Percolator.PEP":0.00255}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (J2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].wGDAGAEYVVESTGVFTTMEk.[A]","Modifications":"N-Term(TMT6plex); K21(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":915.1333,"MH...Da.":2743.38535,"Theo..MH...Da.":2743.37805,"DeltaM..ppm.":2.66,"Deltam.z..Da.":0.00243,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":59.02331,"Average.Reporter.S.N":1.2,"Ion.Inject.Time..ms.":50,"RT..min.":240.681,"First.Scan":126864,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture4_03.raw","File.ID":"F6","Abundance..126":539.92914,"Abundance..127C":442.00562,"Abundance..128N":466.3533,"Abundance..128C":530.72869,"Abundance..129C":981.35953,"Abundance..131":1175.70703,"Ions.Score":8,"Identity.Strict":27,"Identity.Relaxed":20,"Expectation.Value":0.80203,"Percolator.q.Value":0.0003,"Percolator.PEP":0.00252}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (J2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[K].sLQDIIAILGmDELSEEDk.[L]","Modifications":"N-Term(TMT6plex); M11(Oxidation); K19(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":867.80035,"MH...Da.":2601.38651,"Theo..MH...Da.":2601.38247,"DeltaM..ppm.":1.55,"Deltam.z..Da.":0.00135,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":16.38282,"Average.Reporter.S.N":12.6,"Ion.Inject.Time..ms.":50,"RT..min.":236.3222,"First.Scan":124920,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture4_03.raw","File.ID":"F6","Abundance..126":5992.16818,"Abundance..127N":5211.01544,"Abundance..127C":3344.41965,"Abundance..128N":3906.56523,"Abundance..128C":4293.51311,"Abundance..129N":3538.37837,"Abundance..129C":4815.4597,"Abundance..130N":4521.56004,"Abundance..130C":2961.58076,"Abundance..131":3500.79122,"Ions.Score":8,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":1.42175,"Percolator.q.Value":0.0003,"Percolator.PEP":0.00203}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (J2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[K].vELDNMPLr.[G]","Modifications":"N-Term(TMT6plex); R9(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q15233","Master.Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","Protein.Accessions":"Q15233","Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":0.8485,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":663.37061,"MH...Da.":1325.73393,"Theo..MH...Da.":1325.73241,"DeltaM..ppm.":1.15,"Deltam.z..Da.":0.00076,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":10.1244,"Average.Reporter.S.N":24,"Ion.Inject.Time..ms.":0.94,"RT..min.":134.4706,"First.Scan":68765,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture4_03.raw","File.ID":"F6","Abundance..126":270975.10112,"Abundance..127N":293631.80577,"Abundance..127C":238849.23404,"Abundance..128N":358311.34771,"Abundance..128C":344515.20914,"Abundance..129N":213494.04572,"Abundance..129C":280492.48423,"Abundance..130N":334759.56384,"Abundance..130C":315029.18847,"Abundance..131":246604.28629,"Ions.Score":33,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":0.00341,"Percolator.q.Value":0.0003,"Percolator.PEP":0.00256}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (J2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].gIVEFSGkPAAr.[K]","Modifications":"N-Term(TMT6plex); K8(Label); R12(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q15233","Master.Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","Protein.Accessions":"Q15233","Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","X..Missed.Cleavages":1,"Charge":3,"DeltaScore":0.7,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":570.01422,"MH...Da.":1708.02811,"Theo..MH...Da.":1708.02768,"DeltaM..ppm.":0.25,"Deltam.z..Da.":0.00014,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":42.80859,"Average.Reporter.S.N":40.8,"Ion.Inject.Time..ms.":4.233,"RT..min.":98.5447,"First.Scan":47822,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture4_03.raw","File.ID":"F6","Abundance..126":107437.93171,"Abundance..127N":109022.3548,"Abundance..127C":84820.92281,"Abundance..128N":96180.64109,"Abundance..128C":101345.20263,"Abundance..129N":94448.15623,"Abundance..129C":109399.18459,"Abundance..130N":115664.11248,"Abundance..130C":112633.80086,"Abundance..131":132324.2463,"Ions.Score":10,"Identity.Strict":30,"Identity.Relaxed":23,"Expectation.Value":1.07007,"Percolator.q.Value":0.0003,"Percolator.PEP":0.00215}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (M2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].kNEPPLTcPYSLk.[A]","Modifications":"N-Term(TMT6plex); K1(Label); C8(Carbamidomethyl); K13(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q9UGP8","Master.Protein.Descriptions":"Translocation protein SEC63 homolog OS=Homo sapiens GN=SEC63 PE=1 SV=2","Protein.Accessions":"Q9UGP8","Protein.Descriptions":"Translocation protein SEC63 homolog OS=Homo sapiens GN=SEC63 PE=1 SV=2","X..Missed.Cleavages":1,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":750.77515,"MH...Da.":2250.31089,"Theo..MH...Da.":2250.31058,"DeltaM..ppm.":0.13,"Deltam.z..Da.":0.0001,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":13.23344,"Average.Reporter.S.N":16.2,"Ion.Inject.Time..ms.":4.229,"RT..min.":116.269,"First.Scan":55503,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_03.raw","File.ID":"F3","Abundance..126":35638.56289,"Abundance..127N":23113.72703,"Abundance..127C":31942.71437,"Abundance..128N":33597.76445,"Abundance..128C":20626.61934,"Abundance..129N":28051.20376,"Abundance..129C":38257.59944,"Abundance..130N":34965.50465,"Abundance..130C":27749.24866,"Abundance..131":32034.92706,"Ions.Score":19,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.12647,"Percolator.q.Value":0.00034,"Percolator.PEP":0.00285}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (K2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].lFVGNLPPDITEEEMrk.[L]","Modifications":"N-Term(TMT6plex); R16(Label:13C(6)15N(4)); K17(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q15233","Master.Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","Protein.Accessions":"Q15233","Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","X..Missed.Cleavages":1,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":822.12592,"MH...Da.":2464.36319,"Theo..MH...Da.":2464.36407,"DeltaM..ppm.":-0.36,"Deltam.z..Da.":-0.00029,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":100,"Average.Reporter.S.N":9.1,"Ion.Inject.Time..ms.":3.834,"RT..min.":162.657,"First.Scan":86148,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_03.raw","File.ID":"F5","Abundance..126":30194.12221,"Abundance..127N":26839.87359,"Abundance..127C":29378.57738,"Abundance..128N":25474.83584,"Abundance..128C":21623.02594,"Abundance..129N":36836.83997,"Abundance..129C":30813.04419,"Abundance..130N":33539.559,"Abundance..130C":39938.9739,"Abundance..131":30329.95386,"Ions.Score":10,"Identity.Strict":30,"Identity.Relaxed":23,"Expectation.Value":1.19123,"Percolator.q.Value":0.00034,"Percolator.PEP":0.00188}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (K2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].aDIIHAcDIVEDAAIAYGYNNIQMTLPk.[T]","Modifications":"N-Term(TMT6plex); C7(Carbamidomethyl); K28(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q9NSD9","Master.Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","Protein.Accessions":"Q9NSD9","Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","X..Missed.Cleavages":0,"Charge":4,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":897.2215,"MH...Da.":3585.86416,"Theo..MH...Da.":3585.8577,"DeltaM..ppm.":1.8,"Deltam.z..Da.":0.00162,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":0,"Average.Reporter.S.N":3,"Ion.Inject.Time..ms.":6.222,"RT..min.":204.7262,"First.Scan":110303,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_03.raw","File.ID":"F5","Abundance..126":24123.47266,"Abundance..128N":7640.06004,"Abundance..128C":19990.36567,"Abundance..129N":37723.20836,"Abundance..129C":22931.11171,"Abundance..130N":8089.3021,"Abundance..130C":25533.62088,"Abundance..131":33169.26646,"Ions.Score":14,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":0.30796,"Percolator.q.Value":0.00034,"Percolator.PEP":0.00202}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (K2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].lLIEMEQr.[L]","Modifications":"N-Term(TMT6plex); R8(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P12277","Master.Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","Protein.Accessions":"P12277","Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":0.7143,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":635.86682,"MH...Da.":1270.72637,"Theo..MH...Da.":1270.72659,"DeltaM..ppm.":-0.18,"Deltam.z..Da.":-0.00011,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":14.13158,"Average.Reporter.S.N":22.4,"Ion.Inject.Time..ms.":4.5,"RT..min.":138.9768,"First.Scan":72135,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_03.raw","File.ID":"F5","Abundance..126":109351.09016,"Abundance..127N":113302.47241,"Abundance..127C":102885.04213,"Abundance..128N":122666.44051,"Abundance..128C":122833.48242,"Abundance..129N":121659.8947,"Abundance..129C":123046.51911,"Abundance..130N":121858.19952,"Abundance..130C":109520.38165,"Abundance..131":100882.60103,"Ions.Score":35,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.00256,"Percolator.q.Value":0.00034,"Percolator.PEP":0.00176}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (K2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].gQNALVELQTQrPIALELYk.[D]","Modifications":"N-Term(TMT6plex); R12(Label:13C(6)15N(4)); K20(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q9Y450","Master.Protein.Descriptions":"HBS1-like protein OS=Homo sapiens GN=HBS1L PE=1 SV=1","Protein.Accessions":"Q9Y450","Protein.Descriptions":"HBS1-like protein OS=Homo sapiens GN=HBS1L PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":920.87854,"MH...Da.":2760.62107,"Theo..MH...Da.":2760.61429,"DeltaM..ppm.":2.46,"Deltam.z..Da.":0.00226,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":54.13813,"Average.Reporter.S.N":7,"Ion.Inject.Time..ms.":17.866,"RT..min.":163.0438,"First.Scan":86381,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_03.raw","File.ID":"F5","Abundance..126":5282.09369,"Abundance..127N":5299.17613,"Abundance..127C":5631.93837,"Abundance..128N":4240.02795,"Abundance..128C":6360.13106,"Abundance..129N":8490.74764,"Abundance..129C":7893.60507,"Abundance..130N":8540.28444,"Abundance..130C":7166.28195,"Abundance..131":8898.58782,"Ions.Score":5,"Identity.Strict":27,"Identity.Relaxed":20,"Expectation.Value":1.67567,"Percolator.q.Value":0.00034,"Percolator.PEP":0.00182}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (K2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].eGNDLYHEMIESGVINLk.[D]","Modifications":"N-Term(TMT6plex); K18(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":4,"DeltaScore":0.75,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":632.59033,"MH...Da.":2527.3395,"Theo..MH...Da.":2527.33579,"DeltaM..ppm.":1.47,"Deltam.z..Da.":0.00093,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":33.85742,"Average.Reporter.S.N":13.2,"Ion.Inject.Time..ms.":1.35,"RT..min.":173.6624,"First.Scan":92639,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_03.raw","File.ID":"F5","Abundance..126":56460.27644,"Abundance..127N":89508.34936,"Abundance..127C":107300.12229,"Abundance..128N":111148.98878,"Abundance..128C":77868.63898,"Abundance..129N":77242.86399,"Abundance..129C":121580.17363,"Abundance..130N":89855.88139,"Abundance..130C":134522.52901,"Abundance..131":139438.04965,"Ions.Score":4,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":3.01777,"Percolator.q.Value":0.00034,"Percolator.PEP":0.00179}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (K2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].tIAmDGTEGLVr.[G]","Modifications":"N-Term(TMT6plex); M4(Oxidation); R12(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":759.40778,"MH...Da.":1517.80828,"Theo..MH...Da.":1517.80703,"DeltaM..ppm.":0.82,"Deltam.z..Da.":0.00062,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":13.82091,"Average.Reporter.S.N":17.9,"Ion.Inject.Time..ms.":14.449,"RT..min.":99.8957,"First.Scan":49178,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_03.raw","File.ID":"F5","Abundance..126":17247.72307,"Abundance..127N":17928.43026,"Abundance..127C":19159.34897,"Abundance..128N":22058.82146,"Abundance..128C":20511.54182,"Abundance..129N":24657.56504,"Abundance..129C":19159.33519,"Abundance..130N":25123.71448,"Abundance..130C":16707.61354,"Abundance..131":18705.04429,"Ions.Score":29,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":0.00719,"Percolator.q.Value":0.00034,"Percolator.PEP":0.00202}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (N2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].hEHQVmLMr.[Q]","Modifications":"N-Term(TMT6plex); M6(Oxidation); R9(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q15233","Master.Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","Protein.Accessions":"Q15233","Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.375,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":479.25082,"MH...Da.":1435.73792,"Theo..MH...Da.":1435.73751,"DeltaM..ppm.":0.29,"Deltam.z..Da.":0.00014,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":66.5593,"Average.Reporter.S.N":5.8,"Ion.Inject.Time..ms.":50,"RT..min.":24.5943,"First.Scan":7702,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_03.raw","File.ID":"F2","Abundance..126":1712.68566,"Abundance..127N":1970.24929,"Abundance..127C":1749.61618,"Abundance..128N":1879.45051,"Abundance..128C":2374.36518,"Abundance..129N":1642.58035,"Abundance..129C":1351.10641,"Abundance..130N":2948.17266,"Abundance..130C":2028.77211,"Abundance..131":1199.86944,"Ions.Score":8,"Identity.Strict":25,"Identity.Relaxed":18,"Expectation.Value":0.67618,"Percolator.q.Value":0.00034,"Percolator.PEP":0.00307}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (I2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].trEGNDLYHEmIESGVINLk.[D]","Modifications":"N-Term(TMT6plex); R2(Label:13C(6)15N(4)); M11(Oxidation); K20(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":1,"Charge":4,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":703.37799,"MH...Da.":2810.49013,"Theo..MH...Da.":2810.48777,"DeltaM..ppm.":0.84,"Deltam.z..Da.":0.00059,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":29.52869,"Average.Reporter.S.N":19.8,"Ion.Inject.Time..ms.":4.852,"RT..min.":122.6151,"First.Scan":63505,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_01.raw","File.ID":"F7","Abundance..126":53910.18623,"Abundance..127N":55259.1659,"Abundance..127C":47709.22469,"Abundance..128N":56611.63753,"Abundance..128C":48417.66567,"Abundance..129N":40989.61647,"Abundance..129C":51171.74739,"Abundance..130N":54272.53193,"Abundance..130C":68617.35991,"Abundance..131":49184.51071,"Ions.Score":6,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":2.15838,"Percolator.q.Value":0.00035,"Percolator.PEP":0.0025}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (D2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].gIVEFSGkPAAr.[K]","Modifications":"N-Term(TMT6plex); K8(Label); R12(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q15233","Master.Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","Protein.Accessions":"Q15233","Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","X..Missed.Cleavages":1,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":570.01428,"MH...Da.":1708.02829,"Theo..MH...Da.":1708.02768,"DeltaM..ppm.":0.36,"Deltam.z..Da.":0.0002,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":17.08079,"Average.Reporter.S.N":41.1,"Ion.Inject.Time..ms.":4.379,"RT..min.":99.4094,"First.Scan":47699,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_02.raw","File.ID":"F12","Abundance..126":103032.76153,"Abundance..127N":102113.66292,"Abundance..127C":97385.05509,"Abundance..128N":91408.3874,"Abundance..128C":109588.97883,"Abundance..129N":102139.42328,"Abundance..129C":110520.82451,"Abundance..130N":86958.99657,"Abundance..130C":93139.45514,"Abundance..131":102052.22765,"Ions.Score":19,"Identity.Strict":30,"Identity.Relaxed":23,"Expectation.Value":0.13881,"Percolator.q.Value":0.00036,"Percolator.PEP":0.0027}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (D2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].kNEPPLTcPYSLk.[A]","Modifications":"N-Term(TMT6plex); K1(Label); C8(Carbamidomethyl); K13(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q9UGP8","Master.Protein.Descriptions":"Translocation protein SEC63 homolog OS=Homo sapiens GN=SEC63 PE=1 SV=2","Protein.Accessions":"Q9UGP8","Protein.Descriptions":"Translocation protein SEC63 homolog OS=Homo sapiens GN=SEC63 PE=1 SV=2","X..Missed.Cleavages":1,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":750.77582,"MH...Da.":2250.3129,"Theo..MH...Da.":2250.31058,"DeltaM..ppm.":1.03,"Deltam.z..Da.":0.00077,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":59.75544,"Average.Reporter.S.N":24.6,"Ion.Inject.Time..ms.":11.694,"RT..min.":117.7209,"First.Scan":58491,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_02.raw","File.ID":"F12","Abundance..126":23696.38986,"Abundance..127N":22045.05006,"Abundance..127C":24891.61461,"Abundance..128N":20232.73112,"Abundance..128C":27156.32166,"Abundance..129N":21516.67449,"Abundance..129C":22975.57236,"Abundance..130N":24150.94159,"Abundance..130C":22814.37302,"Abundance..131":25632.67437,"Ions.Score":19,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.10483,"Percolator.q.Value":0.00036,"Percolator.PEP":0.00287}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (C2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].mGQMAMGGAMGINNr.[G]","Modifications":"N-Term(TMT6plex); R15(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q15233","Master.Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","Protein.Accessions":"Q15233","Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":889.42523,"MH...Da.":1777.84319,"Theo..MH...Da.":1777.84067,"DeltaM..ppm.":1.42,"Deltam.z..Da.":0.00126,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":0,"Average.Reporter.S.N":6.3,"Ion.Inject.Time..ms.":25.788,"RT..min.":127.697,"First.Scan":63895,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture4_01.raw","File.ID":"F13","Abundance..126":3723.62636,"Abundance..127N":2002.56425,"Abundance..127C":1637.62056,"Abundance..128N":2001.11408,"Abundance..128C":2231.27075,"Abundance..129N":2405.47344,"Abundance..129C":2324.43994,"Abundance..130N":2454.03415,"Abundance..130C":1781.2018,"Abundance..131":3400.62534,"Ions.Score":21,"Identity.Strict":21,"Identity.Relaxed":14,"Expectation.Value":0.011,"Percolator.q.Value":0.0005,"Percolator.PEP":0.00342}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (C2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].wGDAGAEYVVESTGVFTTmEk.[A]","Modifications":"N-Term(TMT6plex); M19(Oxidation); K21(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":920.46503,"MH...Da.":2759.38053,"Theo..MH...Da.":2759.37297,"DeltaM..ppm.":2.74,"Deltam.z..Da.":0.00252,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":51.33432,"Average.Reporter.S.N":7.7,"Ion.Inject.Time..ms.":23.078,"RT..min.":198.3918,"First.Scan":103485,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture4_01.raw","File.ID":"F13","Abundance..126":6190.05727,"Abundance..127N":5196.73901,"Abundance..127C":5676.12188,"Abundance..128N":8048.93169,"Abundance..128C":8437.22902,"Abundance..129N":8919.84604,"Abundance..129C":6090.90915,"Abundance..130N":8692.45795,"Abundance..130C":7424.1698,"Abundance..131":8369.25186,"Ions.Score":17,"Identity.Strict":26,"Identity.Relaxed":19,"Expectation.Value":0.09583,"Percolator.q.Value":0.0005,"Percolator.PEP":0.00262}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (K2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].sIEAVHEDIr.[V]","Modifications":"N-Term(TMT6plex); R10(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P23919","Master.Protein.Descriptions":"Thymidylate kinase OS=Homo sapiens GN=DTYMK PE=1 SV=4","Protein.Accessions":"P23919","Protein.Descriptions":"Thymidylate kinase OS=Homo sapiens GN=DTYMK PE=1 SV=4","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.8333,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":469.92697,"MH...Da.":1407.76636,"Theo..MH...Da.":1407.76688,"DeltaM..ppm.":-0.37,"Deltam.z..Da.":-0.00017,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":23.70079,"Average.Reporter.S.N":55.5,"Ion.Inject.Time..ms.":3.106,"RT..min.":70.3986,"First.Scan":32059,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_03.raw","File.ID":"F5","Abundance..126":226512.22746,"Abundance..127N":267676.06215,"Abundance..127C":248415.86827,"Abundance..128N":242152.23495,"Abundance..128C":211625.15891,"Abundance..129N":238861.75357,"Abundance..129C":257839.73938,"Abundance..130N":236543.33335,"Abundance..130C":223420.5291,"Abundance..131":264541.73457,"Ions.Score":24,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":0.02393,"Percolator.q.Value":0.00055,"Percolator.PEP":0.00348}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (B2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].vIPELNGk.[L]","Modifications":"N-Term(TMT6plex); K8(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.2903,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":445.95453,"MH...Da.":1335.84903,"Theo..MH...Da.":1335.84916,"DeltaM..ppm.":-0.09,"Deltam.z..Da.":-0.00004,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":16.71481,"Average.Reporter.S.N":70.3,"Ion.Inject.Time..ms.":5.325,"RT..min.":130.9169,"First.Scan":67462,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_01.raw","File.ID":"F14","Abundance..126":182154.78188,"Abundance..127N":167179.8508,"Abundance..127C":194723.09068,"Abundance..128N":174439.71951,"Abundance..128C":137392.65203,"Abundance..129N":133768.27216,"Abundance..129C":180799.439,"Abundance..130N":188364.13491,"Abundance..130C":185726.18357,"Abundance..131":145491.15332,"Ions.Score":31,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.00626,"Percolator.q.Value":0.00056,"Percolator.PEP":0.00447}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (K2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].dQNAEQIr.[L]","Modifications":"N-Term(TMT6plex); R8(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q9UGP8","Master.Protein.Descriptions":"Translocation protein SEC63 homolog OS=Homo sapiens GN=SEC63 PE=1 SV=2","Protein.Accessions":"Q9UGP8","Protein.Descriptions":"Translocation protein SEC63 homolog OS=Homo sapiens GN=SEC63 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":0.84,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":606.82495,"MH...Da.":1212.64263,"Theo..MH...Da.":1212.64095,"DeltaM..ppm.":1.38,"Deltam.z..Da.":0.00084,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":12.85003,"Average.Reporter.S.N":12.4,"Ion.Inject.Time..ms.":50,"RT..min.":35.0233,"First.Scan":12744,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_03.raw","File.ID":"F5","Abundance..126":4452.42278,"Abundance..127N":3396.27956,"Abundance..127C":2650.74421,"Abundance..128N":3713.44019,"Abundance..128C":4423.40999,"Abundance..129N":3998.21872,"Abundance..129C":4447.9014,"Abundance..130N":3785.5395,"Abundance..130C":4899.95198,"Abundance..131":4546.89456,"Ions.Score":25,"Identity.Strict":25,"Identity.Relaxed":18,"Expectation.Value":0.01223,"Percolator.q.Value":0.00064,"Percolator.PEP":0.00472}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (A2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].vVDLMAHmASk.[E]","Modifications":"N-Term(TMT6plex); M8(Oxidation); K11(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.8667,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":561.98572,"MH...Da.":1683.9426,"Theo..MH...Da.":1683.94175,"DeltaM..ppm.":0.5,"Deltam.z..Da.":0.00028,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":43.91616,"Average.Reporter.S.N":30.2,"Ion.Inject.Time..ms.":6.215,"RT..min.":111.186,"First.Scan":53373,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_01.raw","File.ID":"F15","Abundance..126":57012.16138,"Abundance..127N":73685.06296,"Abundance..127C":86143.7856,"Abundance..128N":75941.15342,"Abundance..128C":65138.61187,"Abundance..129N":82186.50043,"Abundance..129C":66686.83398,"Abundance..130N":66044.61729,"Abundance..130C":79006.0269,"Abundance..131":84618.9943,"Ions.Score":15,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.27504,"Percolator.q.Value":0.00067,"Percolator.PEP":0.00494}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (O2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].tVLIMELINNVAk.[A]","Modifications":"N-Term(TMT6plex); K13(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":642.06506,"MH...Da.":1924.18064,"Theo..MH...Da.":1924.17968,"DeltaM..ppm.":0.5,"Deltam.z..Da.":0.00032,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":19.10766,"Average.Reporter.S.N":99.4,"Ion.Inject.Time..ms.":18.744,"RT..min.":208.4223,"First.Scan":110808,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_03.raw","File.ID":"F1","Abundance..126":136572.62634,"Abundance..127N":143698.22695,"Abundance..127C":127851.26177,"Abundance..128N":141058.4685,"Abundance..128C":128631.95286,"Abundance..129N":132325.53831,"Abundance..129C":158233.60422,"Abundance..130N":136351.71135,"Abundance..130C":125846.6412,"Abundance..131":129612.60816,"Ions.Score":16,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":0.15317,"Percolator.q.Value":0.00071,"Percolator.PEP":0.00389}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (L2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].qQEEMMr.[R]","Modifications":"N-Term(TMT6plex); R7(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q15233","Master.Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","Protein.Accessions":"Q15233","Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":595.79114,"MH...Da.":1190.575,"Theo..MH...Da.":1190.57346,"DeltaM..ppm.":1.29,"Deltam.z..Da.":0.00077,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":26.83202,"Average.Reporter.S.N":21.8,"Ion.Inject.Time..ms.":50,"RT..min.":43.1151,"First.Scan":16575,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_02.raw","File.ID":"F4","Abundance..126":7273.5661,"Abundance..127N":7847.18752,"Abundance..127C":6029.27988,"Abundance..128N":7261.8195,"Abundance..128C":8473.6554,"Abundance..129N":7951.92743,"Abundance..129C":7365.74879,"Abundance..130N":9000.63943,"Abundance..130C":8678.82308,"Abundance..131":7040.08569,"Ions.Score":21,"Identity.Strict":20,"Identity.Relaxed":13,"Expectation.Value":0.01033,"Percolator.q.Value":0.00071,"Percolator.PEP":0.0067}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (L2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].lHQNIcr.[K]","Modifications":"N-Term(TMT6plex); C6(Carbamidomethyl); R7(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q9NSD9","Master.Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","Protein.Accessions":"Q9NSD9","Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.2353,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":393.8877,"MH...Da.":1179.64853,"Theo..MH...Da.":1179.64935,"DeltaM..ppm.":-0.69,"Deltam.z..Da.":-0.00027,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":15.78793,"Average.Reporter.S.N":24.9,"Ion.Inject.Time..ms.":31.353,"RT..min.":29.4567,"First.Scan":9978,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_02.raw","File.ID":"F4","Abundance..126":6139.6657,"Abundance..127N":10211.21196,"Abundance..127C":10887.85482,"Abundance..128N":7953.10876,"Abundance..128C":10738.17717,"Abundance..129N":9453.29348,"Abundance..129C":8899.66999,"Abundance..130N":8351.61377,"Abundance..130C":8199.40378,"Abundance..131":9247.73749,"Ions.Score":17,"Identity.Strict":27,"Identity.Relaxed":20,"Expectation.Value":0.10676,"Percolator.q.Value":0.00071,"Percolator.PEP":0.00664}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (N2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].sPLAQMEEErr.[E]","Modifications":"N-Term(TMT6plex); R10(Label:13C(6)15N(4)); R11(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q16181","Master.Protein.Descriptions":"Septin-7 OS=Homo sapiens GN=SEPT7 PE=1 SV=2","Protein.Accessions":"Q16181","Protein.Descriptions":"Septin-7 OS=Homo sapiens GN=SEPT7 PE=1 SV=2","X..Missed.Cleavages":1,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":532.28253,"MH...Da.":1594.83304,"Theo..MH...Da.":1594.83234,"DeltaM..ppm.":0.44,"Deltam.z..Da.":0.00023,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":24.24072,"Average.Reporter.S.N":13.2,"Ion.Inject.Time..ms.":21.182,"RT..min.":74.284,"First.Scan":33039,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_03.raw","File.ID":"F2","Abundance..126":9632.48834,"Abundance..127N":7258.39354,"Abundance..127C":10074.36925,"Abundance..128N":6547.98278,"Abundance..128C":7948.82638,"Abundance..129N":8047.51448,"Abundance..129C":8562.66513,"Abundance..130N":8103.92497,"Abundance..130C":6757.17734,"Abundance..131":8354.80478,"Ions.Score":14,"Identity.Strict":27,"Identity.Relaxed":20,"Expectation.Value":0.21997,"Percolator.q.Value":0.00072,"Percolator.PEP":0.00649}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (H2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].cSEGSFLLTTFPr.[P]","Modifications":"N-Term(TMT6plex); C1(Carbamidomethyl); R13(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q15233","Master.Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","Protein.Accessions":"Q15233","Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":585.30658,"MH...Da.":1753.90519,"Theo..MH...Da.":1753.90199,"DeltaM..ppm.":1.82,"Deltam.z..Da.":0.00106,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":0,"Average.Reporter.S.N":23.6,"Ion.Inject.Time..ms.":22.439,"RT..min.":161.7695,"First.Scan":81580,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_02.raw","File.ID":"F8","Abundance..126":38858.40285,"Abundance..127N":40958.20155,"Abundance..127C":38900.36742,"Abundance..128N":45384.55015,"Abundance..128C":44984.69906,"Abundance..129N":46380.62116,"Abundance..129C":37073.87804,"Abundance..130N":33698.50232,"Abundance..130C":48245.90505,"Abundance..131":50296.34909,"Ions.Score":21,"Identity.Strict":26,"Identity.Relaxed":19,"Expectation.Value":0.03225,"Percolator.q.Value":0.00078,"Percolator.PEP":0.00666}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (M2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].lGVLHPDVITk.[F]","Modifications":"N-Term(TMT6plex); K11(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q9NSD9","Master.Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","Protein.Accessions":"Q9NSD9","Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":553.35516,"MH...Da.":1658.05094,"Theo..MH...Da.":1658.04965,"DeltaM..ppm.":0.78,"Deltam.z..Da.":0.00043,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":29.81043,"Average.Reporter.S.N":65.2,"Ion.Inject.Time..ms.":4.44,"RT..min.":128.6691,"First.Scan":62374,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_03.raw","File.ID":"F3","Abundance..126":187225.62648,"Abundance..127N":194448.7252,"Abundance..127C":198823.37131,"Abundance..128N":198433.09362,"Abundance..128C":197218.75476,"Abundance..129N":201369.57078,"Abundance..129C":210393.12879,"Abundance..130N":236933.85524,"Abundance..130C":221745.53101,"Abundance..131":214288.12481,"Ions.Score":18,"Identity.Strict":27,"Identity.Relaxed":20,"Expectation.Value":0.09639,"Percolator.q.Value":0.00079,"Percolator.PEP":0.00538}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (A2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].gIVEFSGkPAAr.[K]","Modifications":"N-Term(TMT6plex); K8(Label); R12(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q15233","Master.Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","Protein.Accessions":"Q15233","Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","X..Missed.Cleavages":1,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":570.01392,"MH...Da.":1708.02719,"Theo..MH...Da.":1708.02768,"DeltaM..ppm.":-0.28,"Deltam.z..Da.":-0.00016,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":18.96021,"Average.Reporter.S.N":41.7,"Ion.Inject.Time..ms.":4.694,"RT..min.":99.9574,"First.Scan":47006,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_01.raw","File.ID":"F15","Abundance..126":85837.64522,"Abundance..127N":91415.41938,"Abundance..127C":94262.75931,"Abundance..128N":87973.69458,"Abundance..128C":100384.85589,"Abundance..129N":84959.40405,"Abundance..129C":103878.34797,"Abundance..130N":85693.59184,"Abundance..130C":89928.86398,"Abundance..131":93315.19552,"Ions.Score":18,"Identity.Strict":30,"Identity.Relaxed":23,"Expectation.Value":0.16684,"Percolator.q.Value":0.0009,"Percolator.PEP":0.00619}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (A2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].fEDYLNAESr.[V]","Modifications":"N-Term(TMT6plex); R10(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q16181","Master.Protein.Descriptions":"Septin-7 OS=Homo sapiens GN=SEPT7 PE=1 SV=2","Protein.Accessions":"Q16181","Protein.Descriptions":"Septin-7 OS=Homo sapiens GN=SEPT7 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":741.86938,"MH...Da.":1482.73149,"Theo..MH...Da.":1482.73016,"DeltaM..ppm.":0.9,"Deltam.z..Da.":0.00067,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":20.87719,"Average.Reporter.S.N":64.4,"Ion.Inject.Time..ms.":5.019,"RT..min.":138.3677,"First.Scan":68902,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_01.raw","File.ID":"F15","Abundance..126":133189.94665,"Abundance..127N":128478.5679,"Abundance..127C":138262.92147,"Abundance..128N":149020.58822,"Abundance..128C":154418.20672,"Abundance..129N":149730.56042,"Abundance..129C":162560.88377,"Abundance..130N":150579.96478,"Abundance..130C":158614.62706,"Abundance..131":167948.48711,"Ions.Score":19,"Identity.Strict":22,"Identity.Relaxed":15,"Expectation.Value":0.02748,"Percolator.q.Value":0.0009,"Percolator.PEP":0.0059}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (A2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[K].iGLFGGAGVGk.[T]","Modifications":"N-Term(TMT6plex); K11(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.7222,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":481.3056,"MH...Da.":1441.90226,"Theo..MH...Da.":1441.90226,"DeltaM..ppm.":0,"Deltam.z..Da.":0,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":9.17923,"Average.Reporter.S.N":208.4,"Ion.Inject.Time..ms.":9.745,"RT..min.":153.6428,"First.Scan":77661,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_01.raw","File.ID":"F15","Abundance..126":189198.45034,"Abundance..127N":163411.72002,"Abundance..127C":165215.39303,"Abundance..128N":182011.08937,"Abundance..128C":160778.36992,"Abundance..129N":149481.92514,"Abundance..129C":191429.05059,"Abundance..130N":175241.68803,"Abundance..130C":154754.04351,"Abundance..131":156876.19907,"Ions.Score":18,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":0.1203,"Percolator.q.Value":0.0009,"Percolator.PEP":0.00601}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (I2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].sHTFPELAYr.[K]","Modifications":"N-Term(TMT6plex); R10(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q9NSD9","Master.Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","Protein.Accessions":"Q9NSD9","Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.8636,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":487.2645,"MH...Da.":1459.77893,"Theo..MH...Da.":1459.77705,"DeltaM..ppm.":1.29,"Deltam.z..Da.":0.00063,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":23.72357,"Average.Reporter.S.N":13.8,"Ion.Inject.Time..ms.":1.859,"RT..min.":90.6415,"First.Scan":44461,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_01.raw","File.ID":"F7","Abundance..126":62272.74269,"Abundance..127N":49674.7979,"Abundance..127C":87033.7663,"Abundance..128N":92721.69018,"Abundance..128C":62114.17099,"Abundance..129N":70289.17295,"Abundance..129C":75621.99831,"Abundance..130N":66519.87661,"Abundance..130C":68247.44027,"Abundance..131":52467.31425,"Ions.Score":22,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":0.04901,"Percolator.q.Value":0.0009,"Percolator.PEP":0.00796}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (I2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[K].aLIEMEk.[Q]","Modifications":"N-Term(TMT6plex); K7(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q15233","Master.Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","Protein.Accessions":"Q15233","Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":0.6757,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":650.39594,"MH...Da.":1299.78459,"Theo..MH...Da.":1299.78378,"DeltaM..ppm.":0.63,"Deltam.z..Da.":0.00041,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":25.45564,"Average.Reporter.S.N":59.3,"Ion.Inject.Time..ms.":1.493,"RT..min.":124.334,"First.Scan":64519,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_01.raw","File.ID":"F7","Abundance..126":524662.89059,"Abundance..127N":586308.26665,"Abundance..127C":522370.319,"Abundance..128N":534051.58256,"Abundance..128C":582170.63039,"Abundance..129N":564275.86129,"Abundance..129C":612132.21117,"Abundance..130N":531374.62399,"Abundance..130C":518079.12449,"Abundance..131":548381.11222,"Ions.Score":37,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":0.00157,"Percolator.q.Value":0.0009,"Percolator.PEP":0.00756}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (G2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].qLELrQEEEr.[R]","Modifications":"N-Term(TMT6plex); R5(Label:13C(6)15N(4)); R10(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q15233","Master.Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","Protein.Accessions":"Q15233","Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","X..Missed.Cleavages":1,"Charge":3,"DeltaScore":0.8,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":526.95691,"MH...Da.":1578.85617,"Theo..MH...Da.":1578.85519,"DeltaM..ppm.":0.62,"Deltam.z..Da.":0.00033,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":44.27523,"Average.Reporter.S.N":40.5,"Ion.Inject.Time..ms.":21.964,"RT..min.":57.7379,"First.Scan":23692,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_02.raw","File.ID":"F9","Abundance..126":33877.21694,"Abundance..127N":29089.49948,"Abundance..127C":36654.0165,"Abundance..128N":32474.48617,"Abundance..128C":33431.67738,"Abundance..129N":31170.47154,"Abundance..129C":31408.67799,"Abundance..130N":29436.74973,"Abundance..130C":31133.15695,"Abundance..131":36382.25484,"Ions.Score":20,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.09396,"Percolator.q.Value":0.00091,"Percolator.PEP":0.00752}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (O2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].dATSrPTDNILIPQLIr.[E]","Modifications":"N-Term(TMT6plex); R5(Label:13C(6)15N(4)); R17(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q9UGP8","Master.Protein.Descriptions":"Translocation protein SEC63 homolog OS=Homo sapiens GN=SEC63 PE=1 SV=2","Protein.Accessions":"Q9UGP8","Protein.Descriptions":"Translocation protein SEC63 homolog OS=Homo sapiens GN=SEC63 PE=1 SV=2","X..Missed.Cleavages":1,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":724.75415,"MH...Da.":2172.2479,"Theo..MH...Da.":2172.24527,"DeltaM..ppm.":1.21,"Deltam.z..Da.":0.00088,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":45.94495,"Average.Reporter.S.N":9.2,"Ion.Inject.Time..ms.":7.197,"RT..min.":151.2565,"First.Scan":78264,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_03.raw","File.ID":"F1","Abundance..126":12182.29651,"Abundance..127N":19825.58274,"Abundance..127C":26725.77041,"Abundance..128N":27561.56204,"Abundance..128C":26933.66822,"Abundance..129N":20608.74738,"Abundance..129C":21064.71601,"Abundance..130N":29152.67902,"Abundance..130C":22604.24135,"Abundance..131":22900.59587,"Ions.Score":18,"Identity.Strict":30,"Identity.Relaxed":23,"Expectation.Value":0.18843,"Percolator.q.Value":0.00092,"Percolator.PEP":0.00481}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (C2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].qFAPIHAEAPEFMEMSVEQEILVTGIk.[V]","Modifications":"N-Term(TMT6plex); K27(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":4,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":878.46625,"MH...Da.":3510.84316,"Theo..MH...Da.":3510.85082,"DeltaM..ppm.":-2.18,"Deltam.z..Da.":-0.00191,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":39.12347,"Average.Reporter.S.N":16.5,"Ion.Inject.Time..ms.":10.938,"RT..min.":197.0485,"First.Scan":102767,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture4_01.raw","File.ID":"F13","Abundance..126":8008.78907,"Abundance..127N":13612.07065,"Abundance..127C":8662.92641,"Abundance..128N":16413.71929,"Abundance..128C":13098.71839,"Abundance..129N":22134.13053,"Abundance..129C":15895.07581,"Abundance..130N":10931.60485,"Abundance..130C":23314.96509,"Abundance..131":21610.31286,"Ions.Score":10,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.83754,"Percolator.q.Value":0.00095,"Percolator.PEP":0.00712}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (A2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].rIIDSAr.[S]","Modifications":"N-Term(TMT6plex); R1(Label:13C(6)15N(4)); R7(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P31947","Master.Protein.Descriptions":"14-3-3 protein sigma OS=Homo sapiens GN=SFN PE=1 SV=1","Protein.Accessions":"P31947","Protein.Descriptions":"14-3-3 protein sigma OS=Homo sapiens GN=SFN PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"DeltaScore":0.3529,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":360.55933,"MH...Da.":1079.66343,"Theo..MH...Da.":1079.66375,"DeltaM..ppm.":-0.3,"Deltam.z..Da.":-0.00011,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":14.9237,"Average.Reporter.S.N":29.5,"Ion.Inject.Time..ms.":12.643,"RT..min.":33.1149,"First.Scan":11542,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_01.raw","File.ID":"F15","Abundance..126":14471.59681,"Abundance..127N":15605.91856,"Abundance..127C":13160.07641,"Abundance..128N":17563.83627,"Abundance..128C":15150.56987,"Abundance..129N":17076.58368,"Abundance..129C":15035.75585,"Abundance..130N":13732.72183,"Abundance..130C":15850.90327,"Abundance..131":14476.7628,"Ions.Score":17,"Identity.Strict":26,"Identity.Relaxed":19,"Expectation.Value":0.08114,"Percolator.q.Value":0.00096,"Percolator.PEP":0.00638}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (A2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].tVDGPSGk.[L]","Modifications":"N-Term(TMT6plex); K8(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":0.9048,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":613.86511,"MH...Da.":1226.72295,"Theo..MH...Da.":1226.72362,"DeltaM..ppm.":-0.55,"Deltam.z..Da.":-0.00034,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":0,"Average.Reporter.S.N":116.4,"Ion.Inject.Time..ms.":36.632,"RT..min.":57.2011,"First.Scan":23349,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_01.raw","File.ID":"F15","Abundance..126":40200.61714,"Abundance..127N":39234.12162,"Abundance..127C":43234.80453,"Abundance..128N":44273.00089,"Abundance..128C":38270.82414,"Abundance..129N":35819.22473,"Abundance..129C":37716.74613,"Abundance..130N":43061.32514,"Abundance..130C":43811.40557,"Abundance..131":34104.71087,"Ions.Score":21,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":0.05936,"Percolator.q.Value":0.00096,"Percolator.PEP":0.00678}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (E2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].lHQNIcr.[K]","Modifications":"N-Term(TMT6plex); C6(Carbamidomethyl); R7(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q9NSD9","Master.Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","Protein.Accessions":"Q9NSD9","Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.6316,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":393.88776,"MH...Da.":1179.64872,"Theo..MH...Da.":1179.64935,"DeltaM..ppm.":-0.53,"Deltam.z..Da.":-0.00021,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":8.51991,"Average.Reporter.S.N":38.3,"Ion.Inject.Time..ms.":20.931,"RT..min.":29.2494,"First.Scan":9398,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_01.raw","File.ID":"F11","Abundance..126":12996.75573,"Abundance..127N":10698.54137,"Abundance..127C":13628.56815,"Abundance..128N":12318.50027,"Abundance..128C":14510.38712,"Abundance..129N":12476.71414,"Abundance..129C":13960.26512,"Abundance..130N":15096.13101,"Abundance..130C":16859.26567,"Abundance..131":14143.0598,"Ions.Score":19,"Identity.Strict":26,"Identity.Relaxed":20,"Expectation.Value":0.06324,"Percolator.q.Value":0.00097,"Percolator.PEP":0.00799}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (J2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].vLSSIEQk.[ST]","Modifications":"N-Term(TMT6plex); K8(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P31947","Master.Protein.Descriptions":"14-3-3 protein sigma OS=Homo sapiens GN=SFN PE=1 SV=1","Protein.Accessions":"P31947","Protein.Descriptions":"14-3-3 protein sigma OS=Homo sapiens GN=SFN PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":2,"m.z..Da.":457.29016,"MH...Da.":1369.85593,"Theo..MH...Da.":1369.85464,"DeltaM..ppm.":0.94,"Deltam.z..Da.":0.00043,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":16.81432,"Average.Reporter.S.N":158.3,"Ion.Inject.Time..ms.":4.955,"RT..min.":110.7296,"First.Scan":54899,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture4_03.raw","File.ID":"F6","Abundance..126":420986.9863,"Abundance..127N":407253.36292,"Abundance..127C":383320.2563,"Abundance..128N":454923.13699,"Abundance..128C":398581.9149,"Abundance..129N":364566.98074,"Abundance..129C":432056.29549,"Abundance..130N":410114.89592,"Abundance..130C":384680.36549,"Abundance..131":406734.2273,"Ions.Score":16,"Identity.Strict":27,"Identity.Relaxed":20,"Expectation.Value":0.15865,"Percolator.q.Value":0.00097,"Percolator.PEP":0.0058}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (J2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].sTPSGFTLDDVIQTGVDNPGHPYImTVGcVAGDEESYEVFk.[D]","Modifications":"N-Term(TMT6plex); M25(Oxidation); C29(Carbamidomethyl); K41(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P12277","Master.Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","Protein.Accessions":"P12277","Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","X..Missed.Cleavages":0,"Charge":4,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":1229.34399,"MH...Da.":4914.35415,"Theo..MH...Da.":4914.36215,"DeltaM..ppm.":-1.63,"Deltam.z..Da.":-0.002,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":0,"Average.Reporter.S.N":1.7,"Ion.Inject.Time..ms.":50,"RT..min.":190.948,"First.Scan":101963,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture4_03.raw","File.ID":"F6","Abundance..126":1057.17349,"Abundance..127N":495.75491,"Abundance..128N":1029.52426,"Abundance..128C":533.72943,"Abundance..129N":977.24845,"Abundance..130N":516.702,"Abundance..130C":1099.6217,"Ions.Score":17,"Identity.Strict":21,"Identity.Relaxed":14,"Expectation.Value":0.03086,"Percolator.q.Value":0.00097,"Percolator.PEP":0.00545}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (J2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].sPLAQMEEErr.[E]","Modifications":"N-Term(TMT6plex); R10(Label:13C(6)15N(4)); R11(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q16181","Master.Protein.Descriptions":"Septin-7 OS=Homo sapiens GN=SEPT7 PE=1 SV=2","Protein.Accessions":"Q16181","Protein.Descriptions":"Septin-7 OS=Homo sapiens GN=SEPT7 PE=1 SV=2","X..Missed.Cleavages":1,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":532.28217,"MH...Da.":1594.83194,"Theo..MH...Da.":1594.83234,"DeltaM..ppm.":-0.25,"Deltam.z..Da.":-0.00013,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":14.68416,"Average.Reporter.S.N":11.9,"Ion.Inject.Time..ms.":4.904,"RT..min.":75.5291,"First.Scan":34570,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture4_03.raw","File.ID":"F6","Abundance..126":16006.86381,"Abundance..127N":18542.89358,"Abundance..127C":10503.00548,"Abundance..128N":15002.79534,"Abundance..128C":22619.02112,"Abundance..129N":20868.59934,"Abundance..129C":19002.83292,"Abundance..130N":21327.97277,"Abundance..130C":20554.56894,"Abundance..131":23436.27811,"Ions.Score":21,"Identity.Strict":27,"Identity.Relaxed":20,"Expectation.Value":0.04802,"Percolator.q.Value":0.00097,"Percolator.PEP":0.00562}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (B2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].drLPLAVVGSNTIIEVNGk.[R]","Modifications":"N-Term(TMT6plex); R2(Label:13C(6)15N(4)); K19(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q16181","Master.Protein.Descriptions":"Septin-7 OS=Homo sapiens GN=SEPT7 PE=1 SV=2","Protein.Accessions":"Q16181","Protein.Descriptions":"Septin-7 OS=Homo sapiens GN=SEPT7 PE=1 SV=2","X..Missed.Cleavages":1,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":824.49603,"MH...Da.":2471.47354,"Theo..MH...Da.":2471.47165,"DeltaM..ppm.":0.77,"Deltam.z..Da.":0.00063,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":14.76695,"Average.Reporter.S.N":6.5,"Ion.Inject.Time..ms.":9.622,"RT..min.":158.3023,"First.Scan":83332,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_01.raw","File.ID":"F14","Abundance..126":9389.64756,"Abundance..127N":10441.34619,"Abundance..127C":6513.17586,"Abundance..128N":9106.96774,"Abundance..128C":10696.91136,"Abundance..129N":11533.27421,"Abundance..129C":8177.67288,"Abundance..130N":6907.99784,"Abundance..130C":14497.50231,"Abundance..131":8506.22417,"Ions.Score":9,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":0.86166,"Percolator.q.Value":0.00103,"Percolator.PEP":0.00832}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (B2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].vIISAPSADAPmFVMGVNHEk.[Y]","Modifications":"N-Term(TMT6plex); M12(Oxidation); K21(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":899.15363,"MH...Da.":2695.44632,"Theo..MH...Da.":2695.4443,"DeltaM..ppm.":0.75,"Deltam.z..Da.":0.00067,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":59.13591,"Average.Reporter.S.N":5.6,"Ion.Inject.Time..ms.":16.382,"RT..min.":160.6875,"First.Scan":84728,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_01.raw","File.ID":"F14","Abundance..126":11267.63467,"Abundance..127N":8945.26526,"Abundance..127C":6475.17289,"Abundance..128N":12256.20857,"Abundance..128C":14323.31616,"Abundance..129N":6834.80109,"Abundance..129C":10597.97013,"Abundance..130N":11750.73255,"Abundance..130C":8419.00856,"Abundance..131":13020.84509,"Ions.Score":0,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":7.82382,"Percolator.q.Value":0.00103,"Percolator.PEP":0.00856}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (J2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[K].aLIEMEk.[Q]","Modifications":"N-Term(TMT6plex); K7(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q15233","Master.Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","Protein.Accessions":"Q15233","Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":0.8235,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":650.39587,"MH...Da.":1299.78447,"Theo..MH...Da.":1299.78378,"DeltaM..ppm.":0.53,"Deltam.z..Da.":0.00035,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":25.18069,"Average.Reporter.S.N":41,"Ion.Inject.Time..ms.":0.925,"RT..min.":126.2077,"First.Scan":63913,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture4_03.raw","File.ID":"F6","Abundance..126":515451.01778,"Abundance..127N":422671.87225,"Abundance..127C":433236.49014,"Abundance..128N":469399.27413,"Abundance..128C":498186.58395,"Abundance..129N":479565.41338,"Abundance..129C":608052.48177,"Abundance..130N":536364.40626,"Abundance..130C":542365.64698,"Abundance..131":418031.18045,"Ions.Score":34,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":0.0031,"Percolator.q.Value":0.00105,"Percolator.PEP":0.00652}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (B2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].wGDAGAEYVVESTGVFTTmEk.[A]","Modifications":"N-Term(TMT6plex); M19(Oxidation); K21(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":920.46021,"MH...Da.":2759.36606,"Theo..MH...Da.":2759.37297,"DeltaM..ppm.":-2.5,"Deltam.z..Da.":-0.0023,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":73.21525,"Average.Reporter.S.N":5.2,"Ion.Inject.Time..ms.":24.637,"RT..min.":186.2157,"First.Scan":99456,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_01.raw","File.ID":"F14","Abundance..126":5558.02285,"Abundance..127N":14156.56037,"Abundance..127C":8208.42063,"Abundance..128N":6482.10978,"Abundance..128C":2950.39392,"Abundance..129N":8679.81254,"Abundance..129C":4637.1537,"Abundance..130N":7935.9322,"Abundance..130C":1841.44142,"Abundance..131":9490.58158,"Ions.Score":11,"Identity.Strict":26,"Identity.Relaxed":19,"Expectation.Value":0.30746,"Percolator.q.Value":0.00105,"Percolator.PEP":0.00894}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (B2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].qFAPIHAEAPEFMEMSVEQEILVTGIk.[V]","Modifications":"N-Term(TMT6plex); K27(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":4,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":878.46912,"MH...Da.":3510.85463,"Theo..MH...Da.":3510.85082,"DeltaM..ppm.":1.09,"Deltam.z..Da.":0.00095,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":0.51229,"Average.Reporter.S.N":0.5,"Ion.Inject.Time..ms.":0.736,"RT..min.":197.1106,"First.Scan":105493,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_01.raw","File.ID":"F14","Abundance..127N":73819.22656,"Abundance..128C":29231.49414,"Ions.Score":25,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.02754,"Percolator.q.Value":0.00122,"Percolator.PEP":0.01042}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (E2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].eVLGDAVPDEILIEAVLk.[N]","Modifications":"N-Term(TMT6plex); K18(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q9Y450","Master.Protein.Descriptions":"HBS1-like protein OS=Homo sapiens GN=HBS1L PE=1 SV=1","Protein.Accessions":"Q9Y450","Protein.Descriptions":"HBS1-like protein OS=Homo sapiens GN=HBS1L PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":797.14221,"MH...Da.":2389.41208,"Theo..MH...Da.":2389.40855,"DeltaM..ppm.":1.48,"Deltam.z..Da.":0.00118,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":18.34918,"Average.Reporter.S.N":12.1,"Ion.Inject.Time..ms.":50,"RT..min.":220.1037,"First.Scan":110595,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_01.raw","File.ID":"F11","Abundance..126":4036.21107,"Abundance..127N":4906.55211,"Abundance..127C":4393.175,"Abundance..128N":4179.09538,"Abundance..128C":3082.61915,"Abundance..129N":3851.12625,"Abundance..129C":3853.51659,"Abundance..130N":4555.5122,"Abundance..130C":3306.85528,"Abundance..131":4211.07048,"Ions.Score":15,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.24033,"Percolator.q.Value":0.00122,"Percolator.PEP":0.01171}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (N2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].gFQQILAGEYDHLPEQAFYmVGPIEEAVAk.[A]","Modifications":"N-Term(TMT6plex); M20(Oxidation); K30(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":4,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":958.99908,"MH...Da.":3832.97451,"Theo..MH...Da.":3832.97517,"DeltaM..ppm.":-0.17,"Deltam.z..Da.":-0.00016,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":34.64329,"Average.Reporter.S.N":8.9,"Ion.Inject.Time..ms.":19.473,"RT..min.":210.1683,"First.Scan":109276,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_03.raw","File.ID":"F2","Abundance..126":3021.1912,"Abundance..127N":4543.44876,"Abundance..127C":3471.95704,"Abundance..128N":3519.49232,"Abundance..128C":3674.64719,"Abundance..129N":3114.6458,"Abundance..129C":3814.08182,"Abundance..130N":3541.02079,"Abundance..130C":2191.622,"Abundance..131":3607.03067,"Ions.Score":6,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":1.94021,"Percolator.q.Value":0.0013,"Percolator.PEP":0.0088}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (O2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].wGDAGAEYVVESTGVFTTMEk.[A]","Modifications":"N-Term(TMT6plex); K21(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":915.13062,"MH...Da.":2743.37729,"Theo..MH...Da.":2743.37805,"DeltaM..ppm.":-0.28,"Deltam.z..Da.":-0.00025,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":25.92314,"Ion.Inject.Time..ms.":50,"RT..min.":258.4475,"First.Scan":134088,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_03.raw","File.ID":"F1","Ions.Score":6,"Identity.Strict":27,"Identity.Relaxed":20,"Expectation.Value":1.25995,"Percolator.q.Value":0.0013,"Percolator.PEP":0.00854}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (J2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].sPLAQmEEErr.[E]","Modifications":"N-Term(TMT6plex); M6(Oxidation); R10(Label:13C(6)15N(4)); R11(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q16181","Master.Protein.Descriptions":"Septin-7 OS=Homo sapiens GN=SEPT7 PE=1 SV=2","Protein.Accessions":"Q16181","Protein.Descriptions":"Septin-7 OS=Homo sapiens GN=SEPT7 PE=1 SV=2","X..Missed.Cleavages":1,"Charge":3,"DeltaScore":0.4,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":537.61407,"MH...Da.":1610.82767,"Theo..MH...Da.":1610.82726,"DeltaM..ppm.":0.26,"Deltam.z..Da.":0.00014,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":54.31366,"Average.Reporter.S.N":22.5,"Ion.Inject.Time..ms.":19.889,"RT..min.":41.1507,"First.Scan":15696,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture4_03.raw","File.ID":"F6","Abundance..126":12266.74686,"Abundance..127N":11699.63934,"Abundance..127C":10744.33413,"Abundance..128N":12126.79299,"Abundance..128C":13639.2232,"Abundance..129N":11237.61226,"Abundance..129C":13079.05061,"Abundance..130N":14761.92149,"Abundance..130C":13764.73566,"Abundance..131":12185.27547,"Ions.Score":5,"Identity.Strict":27,"Identity.Relaxed":20,"Expectation.Value":1.56676,"Percolator.q.Value":0.00131,"Percolator.PEP":0.01179}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (D2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].iMNVIGEPIDEr.[G]","Modifications":"N-Term(TMT6plex); R12(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":812.94495,"MH...Da.":1624.88262,"Theo..MH...Da.":1624.88053,"DeltaM..ppm.":1.29,"Deltam.z..Da.":0.00104,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":0,"Average.Reporter.S.N":15.7,"Ion.Inject.Time..ms.":11.837,"RT..min.":154.9731,"First.Scan":80458,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_02.raw","File.ID":"F12","Abundance..126":23198.91731,"Abundance..127N":22480.11068,"Abundance..127C":21704.17249,"Abundance..128N":29488.01214,"Abundance..128C":23497.68255,"Abundance..129N":30396.59751,"Abundance..129C":29192.93777,"Abundance..130N":25681.2731,"Abundance..130C":26847.30693,"Abundance..131":22165.94071,"Ions.Score":10,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.90952,"Percolator.q.Value":0.00131,"Percolator.PEP":0.01118}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (M2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[K].aLIEMEk.[Q]","Modifications":"N-Term(TMT6plex); K7(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q15233","Master.Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","Protein.Accessions":"Q15233","Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":0.8108,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":650.39557,"MH...Da.":1299.78386,"Theo..MH...Da.":1299.78378,"DeltaM..ppm.":0.06,"Deltam.z..Da.":0.00004,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":29.0032,"Average.Reporter.S.N":65.2,"Ion.Inject.Time..ms.":2.695,"RT..min.":125.9083,"First.Scan":60850,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_03.raw","File.ID":"F3","Abundance..126":378462.97239,"Abundance..127N":416884.7051,"Abundance..127C":431318.14088,"Abundance..128N":436899.91925,"Abundance..128C":358476.21678,"Abundance..129N":378159.31641,"Abundance..129C":404744.51543,"Abundance..130N":435616.19831,"Abundance..130C":413350.55466,"Abundance..131":362530.83522,"Ions.Score":37,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":0.00173,"Percolator.q.Value":0.00132,"Percolator.PEP":0.00912}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (C2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[K].gFQQILAGEYDHLPEQAFYMVGPIEEAVAk.[A]","Modifications":"N-Term(TMT6plex); K30(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":4,"DeltaScore":0.6667,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":955.00275,"MH...Da.":3816.98916,"Theo..MH...Da.":3816.98025,"DeltaM..ppm.":2.33,"Deltam.z..Da.":0.00223,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":12.25118,"Average.Reporter.S.N":18.7,"Ion.Inject.Time..ms.":13.815,"RT..min.":212.7294,"First.Scan":110761,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture4_01.raw","File.ID":"F13","Abundance..126":23248.76455,"Abundance..127N":27003.17155,"Abundance..127C":24710.10384,"Abundance..128N":27405.42217,"Abundance..128C":27239.06078,"Abundance..129N":30866.91963,"Abundance..129C":34489.42572,"Abundance..130N":28551.14563,"Abundance..130C":28068.07309,"Abundance..131":32456.43776,"Ions.Score":3,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":4.07189,"Percolator.q.Value":0.00137,"Percolator.PEP":0.01413}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (I2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[K].sIEAVHEDIr.[V]","Modifications":"N-Term(TMT6plex); R10(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P23919","Master.Protein.Descriptions":"Thymidylate kinase OS=Homo sapiens GN=DTYMK PE=1 SV=4","Protein.Accessions":"P23919","Protein.Descriptions":"Thymidylate kinase OS=Homo sapiens GN=DTYMK PE=1 SV=4","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.8696,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":469.92697,"MH...Da.":1407.76636,"Theo..MH...Da.":1407.76688,"DeltaM..ppm.":-0.37,"Deltam.z..Da.":-0.00017,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":20.53642,"Average.Reporter.S.N":54.3,"Ion.Inject.Time..ms.":2.995,"RT..min.":70.0914,"First.Scan":32497,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_01.raw","File.ID":"F7","Abundance..126":193692.8821,"Abundance..127N":222522.0766,"Abundance..127C":230308.34715,"Abundance..128N":233440.96039,"Abundance..128C":214218.81823,"Abundance..129N":234529.87438,"Abundance..129C":224868.21439,"Abundance..130N":211753.61881,"Abundance..130C":212183.4469,"Abundance..131":208309.71877,"Ions.Score":23,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":0.03118,"Percolator.q.Value":0.00138,"Percolator.PEP":0.01141}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (I2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].sQVLDDEDSNNITVGSLVTVLVk.[L]","Modifications":"N-Term(TMT6plex); K23(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q9UGP8","Master.Protein.Descriptions":"Translocation protein SEC63 homolog OS=Homo sapiens GN=SEC63 PE=1 SV=2","Protein.Accessions":"Q9UGP8","Protein.Descriptions":"Translocation protein SEC63 homolog OS=Homo sapiens GN=SEC63 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.9,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":971.21082,"MH...Da.":2911.61789,"Theo..MH...Da.":2911.61195,"DeltaM..ppm.":2.04,"Deltam.z..Da.":0.00198,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":67.78165,"Average.Reporter.S.N":10.1,"Ion.Inject.Time..ms.":23.074,"RT..min.":200.7375,"First.Scan":109599,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_01.raw","File.ID":"F7","Abundance..126":6420.45174,"Abundance..127N":5340.92257,"Abundance..127C":6725.99654,"Abundance..128N":5727.77543,"Abundance..128C":7639.69408,"Abundance..129N":5042.30389,"Abundance..129C":6572.27673,"Abundance..130N":6325.03115,"Abundance..130C":4561.27167,"Abundance..131":6147.83494,"Ions.Score":10,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":1.0196,"Percolator.q.Value":0.00138,"Percolator.PEP":0.01163}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (I2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].mGQMAMGGAMGINNr.[G]","Modifications":"N-Term(TMT6plex); R15(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q15233","Master.Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","Protein.Accessions":"Q15233","Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":889.42511,"MH...Da.":1777.84294,"Theo..MH...Da.":1777.84067,"DeltaM..ppm.":1.28,"Deltam.z..Da.":0.00114,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":27.87134,"Average.Reporter.S.N":3.9,"Ion.Inject.Time..ms.":11.341,"RT..min.":125.3022,"First.Scan":65101,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_01.raw","File.ID":"F7","Abundance..126":2974.92214,"Abundance..127N":1816.86227,"Abundance..127C":2704.20269,"Abundance..128N":3635.14001,"Abundance..128C":3005.75315,"Abundance..129N":2643.00716,"Abundance..129C":1965.33729,"Abundance..130N":3644.20113,"Abundance..130C":2345.20638,"Abundance..131":3313.99012,"Ions.Score":4,"Identity.Strict":21,"Identity.Relaxed":14,"Expectation.Value":0.53744,"Percolator.q.Value":0.00138,"Percolator.PEP":0.01166}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (I2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].yENGAFQEr.[A]","Modifications":"N-Term(TMT6plex); R9(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P23919","Master.Protein.Descriptions":"Thymidylate kinase OS=Homo sapiens GN=DTYMK PE=1 SV=4","Protein.Accessions":"P23919","Protein.Descriptions":"Thymidylate kinase OS=Homo sapiens GN=DTYMK PE=1 SV=4","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":0.8,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":676.83661,"MH...Da.":1352.66594,"Theo..MH...Da.":1352.66716,"DeltaM..ppm.":-0.9,"Deltam.z..Da.":-0.00061,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":30.01604,"Average.Reporter.S.N":14.9,"Ion.Inject.Time..ms.":16.879,"RT..min.":71.3425,"First.Scan":33225,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_01.raw","File.ID":"F7","Abundance..126":15306.38005,"Abundance..127N":11672.57069,"Abundance..127C":12069.75657,"Abundance..128N":10029.57921,"Abundance..128C":14565.95583,"Abundance..129N":13141.58113,"Abundance..129C":14367.71665,"Abundance..130N":13682.11629,"Abundance..130C":13229.18753,"Abundance..131":14696.80667,"Ions.Score":35,"Identity.Strict":21,"Identity.Relaxed":14,"Expectation.Value":0.00047,"Percolator.q.Value":0.0014,"Percolator.PEP":0.01172}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (N2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].hEHQVmLmr.[Q]","Modifications":"N-Term(TMT6plex); M6(Oxidation); M8(Oxidation); R9(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q15233","Master.Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","Protein.Accessions":"Q15233","Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","X..Missed.Cleavages":0,"Charge":4,"DeltaScore":0.7857,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":363.68845,"MH...Da.":1451.73195,"Theo..MH...Da.":1451.73242,"DeltaM..ppm.":-0.32,"Deltam.z..Da.":-0.00012,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":42.37738,"Average.Reporter.S.N":15.4,"Ion.Inject.Time..ms.":29.63,"RT..min.":14.7012,"First.Scan":3337,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_03.raw","File.ID":"F2","Abundance..126":4469.71286,"Abundance..127N":3609.545,"Abundance..127C":6289.495,"Abundance..128N":5388.81374,"Abundance..128C":6140.36981,"Abundance..129N":5284.22831,"Abundance..129C":5571.58371,"Abundance..130N":5945.43473,"Abundance..130C":4514.473,"Abundance..131":6582.09585,"Ions.Score":14,"Identity.Strict":24,"Identity.Relaxed":17,"Expectation.Value":0.12425,"Percolator.q.Value":0.0014,"Percolator.PEP":0.00938}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (N2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].lGVLHPDVITk.[F]","Modifications":"N-Term(TMT6plex); K11(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q9NSD9","Master.Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","Protein.Accessions":"Q9NSD9","Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.8077,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":553.3551,"MH...Da.":1658.05075,"Theo..MH...Da.":1658.04965,"DeltaM..ppm.":0.67,"Deltam.z..Da.":0.00037,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":39.73294,"Average.Reporter.S.N":44.2,"Ion.Inject.Time..ms.":2.813,"RT..min.":128.3503,"First.Scan":63483,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_03.raw","File.ID":"F2","Abundance..126":119553.47365,"Abundance..127N":115359.03939,"Abundance..127C":133318.77327,"Abundance..128N":137073.42211,"Abundance..128C":112435.33245,"Abundance..129N":150632.70967,"Abundance..129C":126135.93292,"Abundance..130N":111067.24792,"Abundance..130C":145307.56961,"Abundance..131":154894.7577,"Ions.Score":26,"Identity.Strict":27,"Identity.Relaxed":20,"Expectation.Value":0.01503,"Percolator.q.Value":0.0014,"Percolator.PEP":0.00928}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (C2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].eVLGDAVPDEILIEAVLk.[N]","Modifications":"N-Term(TMT6plex); K18(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q9Y450","Master.Protein.Descriptions":"HBS1-like protein OS=Homo sapiens GN=HBS1L PE=1 SV=1","Protein.Accessions":"Q9Y450","Protein.Descriptions":"HBS1-like protein OS=Homo sapiens GN=HBS1L PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.9333,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":797.14185,"MH...Da.":2389.41098,"Theo..MH...Da.":2389.40855,"DeltaM..ppm.":1.02,"Deltam.z..Da.":0.00081,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":11.7719,"Average.Reporter.S.N":10.2,"Ion.Inject.Time..ms.":35.045,"RT..min.":219.4667,"First.Scan":114037,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture4_01.raw","File.ID":"F13","Abundance..126":4725.18475,"Abundance..127N":5058.436,"Abundance..127C":4329.33892,"Abundance..128N":3301.05644,"Abundance..128C":3982.18055,"Abundance..129N":3795.47055,"Abundance..129C":4616.86285,"Abundance..130N":3866.48933,"Abundance..130C":3800.55845,"Abundance..131":5006.02036,"Ions.Score":15,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.24552,"Percolator.q.Value":0.00144,"Percolator.PEP":0.01628}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (N2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].iMQLLDVPPGEDk.[G]","Modifications":"N-Term(TMT6plex); K13(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q9NSD9","Master.Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","Protein.Accessions":"Q9NSD9","Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":641.03717,"MH...Da.":1921.09696,"Theo..MH...Da.":1921.09601,"DeltaM..ppm.":0.5,"Deltam.z..Da.":0.00032,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":43.85967,"Average.Reporter.S.N":23.9,"Ion.Inject.Time..ms.":17.723,"RT..min.":176.1606,"First.Scan":90870,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_03.raw","File.ID":"F2","Abundance..126":11493.45654,"Abundance..127N":11436.45265,"Abundance..127C":12293.09331,"Abundance..128N":14288.06748,"Abundance..128C":11759.22984,"Abundance..129N":11762.96048,"Abundance..129C":15145.54658,"Abundance..130N":14075.77749,"Abundance..130C":10892.52313,"Abundance..131":11817.811,"Ions.Score":8,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":1.43624,"Percolator.q.Value":0.00154,"Percolator.PEP":0.0117}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (J2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].sHTFPELAYr.[K]","Modifications":"N-Term(TMT6plex); R10(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q9NSD9","Master.Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","Protein.Accessions":"Q9NSD9","Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.6364,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":487.2644,"MH...Da.":1459.77866,"Theo..MH...Da.":1459.77705,"DeltaM..ppm.":1.1,"Deltam.z..Da.":0.00054,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":20.13127,"Average.Reporter.S.N":17.2,"Ion.Inject.Time..ms.":3.97,"RT..min.":91.2943,"First.Scan":43618,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture4_03.raw","File.ID":"F6","Abundance..126":85842.98654,"Abundance..127N":74250.3356,"Abundance..127C":55381.63006,"Abundance..128N":88111.12693,"Abundance..128C":68155.00399,"Abundance..129N":63375.96025,"Abundance..129C":76142.49777,"Abundance..130N":87021.12326,"Abundance..130C":73317.19199,"Abundance..131":67045.09403,"Ions.Score":11,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":0.58926,"Percolator.q.Value":0.00154,"Percolator.PEP":0.01537}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (J2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].dQNAEQIr.[L]","Modifications":"N-Term(TMT6plex); R8(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q9UGP8","Master.Protein.Descriptions":"Translocation protein SEC63 homolog OS=Homo sapiens GN=SEC63 PE=1 SV=2","Protein.Accessions":"Q9UGP8","Protein.Descriptions":"Translocation protein SEC63 homolog OS=Homo sapiens GN=SEC63 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":0.7647,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":606.82532,"MH...Da.":1212.64336,"Theo..MH...Da.":1212.64095,"DeltaM..ppm.":1.99,"Deltam.z..Da.":0.0012,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":33.95338,"Average.Reporter.S.N":5.1,"Ion.Inject.Time..ms.":50,"RT..min.":34.9947,"First.Scan":12570,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture4_03.raw","File.ID":"F6","Abundance..126":1480.63299,"Abundance..127C":1537.1353,"Abundance..128N":2428.58625,"Abundance..128C":2201.83115,"Abundance..129N":1342.0833,"Abundance..129C":2161.89339,"Abundance..130N":1937.14408,"Abundance..130C":1971.28332,"Abundance..131":1978.31842,"Ions.Score":17,"Identity.Strict":25,"Identity.Relaxed":18,"Expectation.Value":0.07737,"Percolator.q.Value":0.00154,"Percolator.PEP":0.01475}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (J2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].aGEVFIHk.[D]","Modifications":"N-Term(TMT6plex); K8(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q15233","Master.Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","Protein.Accessions":"Q15233","Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.6,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":456.28314,"MH...Da.":1366.83487,"Theo..MH...Da.":1366.83384,"DeltaM..ppm.":0.75,"Deltam.z..Da.":0.00034,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":17.96575,"Average.Reporter.S.N":46.4,"Ion.Inject.Time..ms.":0.564,"RT..min.":92.3798,"First.Scan":44257,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture4_03.raw","File.ID":"F6","Abundance..126":1903998.15703,"Abundance..127N":1884353.58464,"Abundance..127C":1535446.90015,"Abundance..128N":2052343.08241,"Abundance..128C":2110703.62279,"Abundance..129N":1636363.85786,"Abundance..129C":2128602.83061,"Abundance..130N":1823829.88348,"Abundance..130C":1890045.63567,"Abundance..131":1769601.84231,"Ions.Score":20,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":0.07315,"Percolator.q.Value":0.00168,"Percolator.PEP":0.01639}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (K2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].qASEGPLk.[G]","Modifications":"N-Term(TMT6plex); K8(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":0.84,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":648.39441,"MH...Da.":1295.78154,"Theo..MH...Da.":1295.78147,"DeltaM..ppm.":0.05,"Deltam.z..Da.":0.00004,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":59.51254,"Average.Reporter.S.N":35.4,"Ion.Inject.Time..ms.":3.933,"RT..min.":66.6678,"First.Scan":29927,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_03.raw","File.ID":"F5","Abundance..126":155409.2898,"Abundance..127N":160595.54652,"Abundance..127C":168641.34082,"Abundance..128N":152469.55373,"Abundance..128C":156350.21658,"Abundance..129N":142162.63028,"Abundance..129C":151765.43951,"Abundance..130N":172422.85161,"Abundance..130C":153220.25932,"Abundance..131":143460.68808,"Ions.Score":25,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":0.02153,"Percolator.q.Value":0.00169,"Percolator.PEP":0.01299}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (K2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].wGDAGAEYVVESTGVFTTMEk.[A]","Modifications":"N-Term(TMT6plex); K21(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":915.13281,"MH...Da.":2743.38388,"Theo..MH...Da.":2743.37805,"DeltaM..ppm.":2.13,"Deltam.z..Da.":0.00194,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":56.05129,"Average.Reporter.S.N":2.3,"Ion.Inject.Time..ms.":50,"RT..min.":240.8426,"First.Scan":127889,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_03.raw","File.ID":"F5","Abundance..126":1511.16504,"Abundance..128N":1219.04703,"Abundance..128C":422.11978,"Abundance..129N":1596.91648,"Abundance..129C":801.56252,"Abundance..130N":1029.40025,"Abundance..130C":528.15731,"Abundance..131":521.62431,"Ions.Score":1,"Identity.Strict":27,"Identity.Relaxed":20,"Expectation.Value":4.28907,"Percolator.q.Value":0.00211,"Percolator.PEP":0.01765}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (O2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].aDTLTPEEcQQFk.[K]","Modifications":"N-Term(TMT6plex); C9(Carbamidomethyl); K13(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q16181","Master.Protein.Descriptions":"Septin-7 OS=Homo sapiens GN=SEPT7 PE=1 SV=2","Protein.Accessions":"Q16181","Protein.Descriptions":"Septin-7 OS=Homo sapiens GN=SEPT7 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":678.35419,"MH...Da.":2033.04801,"Theo..MH...Da.":2033.05051,"DeltaM..ppm.":-1.23,"Deltam.z..Da.":-0.00083,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":22.37907,"Average.Reporter.S.N":5.1,"Ion.Inject.Time..ms.":8.684,"RT..min.":121.7346,"First.Scan":61097,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_03.raw","File.ID":"F1","Abundance..126":10533.41609,"Abundance..127N":10479.82616,"Abundance..127C":8158.14787,"Abundance..128N":4987.52706,"Abundance..128C":10039.64509,"Abundance..129N":6531.39943,"Abundance..129C":5562.48085,"Abundance..130N":8942.72029,"Abundance..130C":4459.05285,"Abundance..131":11087.88527,"Ions.Score":10,"Identity.Strict":27,"Identity.Relaxed":20,"Expectation.Value":0.46163,"Percolator.q.Value":0.00212,"Percolator.PEP":0.01886}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (K2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[K].vVDLLAPYAk.[G]","Modifications":"N-Term(TMT6plex); K10(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.7308,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":518.99664,"MH...Da.":1554.97538,"Theo..MH...Da.":1554.97509,"DeltaM..ppm.":0.19,"Deltam.z..Da.":0.0001,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":24.6289,"Average.Reporter.S.N":96.6,"Ion.Inject.Time..ms.":1.547,"RT..min.":170.8897,"First.Scan":91008,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_03.raw","File.ID":"F5","Abundance..126":857170.80153,"Abundance..127N":912672.94309,"Abundance..127C":852028.91877,"Abundance..128N":951535.12918,"Abundance..128C":810542.51417,"Abundance..129N":883116.99225,"Abundance..129C":858398.97037,"Abundance..130N":845647.47364,"Abundance..130C":923477.30979,"Abundance..131":863248.55714,"Ions.Score":26,"Identity.Strict":27,"Identity.Relaxed":21,"Expectation.Value":0.01518,"Percolator.q.Value":0.00218,"Percolator.PEP":0.02199}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (E4)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[K].qASEGPLk.[G]","Modifications":"N-Term(TMT6plex); K8(TMT6plex)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":0.7143,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":644.38837,"MH...Da.":1287.76946,"Theo..MH...Da.":1287.76727,"DeltaM..ppm.":1.7,"Deltam.z..Da.":0.00109,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":14.06481,"Average.Reporter.S.N":32.6,"Ion.Inject.Time..ms.":42.959,"RT..min.":67.4404,"First.Scan":28458,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_01.raw","File.ID":"F11","Abundance..126":13882.68362,"Abundance..127N":14592.21233,"Abundance..127C":16510.20098,"Abundance..128N":19983.3353,"Abundance..128C":16400.91339,"Abundance..129N":15302.98594,"Abundance..129C":20163.03041,"Abundance..130N":14795.57442,"Abundance..130C":18653.27798,"Abundance..131":14242.46237,"Ions.Score":28,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.01323,"Percolator.q.Value":0.00218,"Percolator.PEP":0.1119}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (C4)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].dNLTLWTADNAGEEGGEAPQEPQS.[-]","Modifications":"N-Term(TMT6plex)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P31947","Master.Protein.Descriptions":"14-3-3 protein sigma OS=Homo sapiens GN=SFN PE=1 SV=1","Protein.Accessions":"P31947","Protein.Descriptions":"14-3-3 protein sigma OS=Homo sapiens GN=SFN PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":920.0943,"MH...Da.":2758.26834,"Theo..MH...Da.":2758.26413,"DeltaM..ppm.":1.53,"Deltam.z..Da.":0.00141,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":7.60941,"Average.Reporter.S.N":26.8,"Ion.Inject.Time..ms.":7.981,"RT..min.":174.7608,"First.Scan":90588,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture4_01.raw","File.ID":"F13","Abundance..126":33321.03245,"Abundance..127N":37085.7862,"Abundance..127C":37509.44484,"Abundance..128N":45842.02965,"Abundance..128C":38623.01975,"Abundance..129N":39192.30923,"Abundance..129C":44137.24488,"Abundance..130N":40322.68096,"Abundance..130C":37622.08664,"Abundance..131":34012.20264,"Ions.Score":33,"Identity.Strict":20,"Identity.Relaxed":13,"Expectation.Value":0.00065,"Percolator.q.Value":0.00219,"Percolator.PEP":0.04844}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (I2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].wGDAGAEYVVESTGVFTTMEk.[A]","Modifications":"N-Term(TMT6plex); K21(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":915.13354,"MH...Da.":2743.38608,"Theo..MH...Da.":2743.37805,"DeltaM..ppm.":2.93,"Deltam.z..Da.":0.00268,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":34.13006,"Ion.Inject.Time..ms.":50,"RT..min.":259.6567,"First.Scan":138666,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_01.raw","File.ID":"F7","Ions.Score":7,"Identity.Strict":27,"Identity.Relaxed":20,"Expectation.Value":1.12732,"Percolator.q.Value":0.0022,"Percolator.PEP":0.02133}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (I2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].aGEVFIHk.[D]","Modifications":"N-Term(TMT6plex); K8(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q15233","Master.Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","Protein.Accessions":"Q15233","Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.7143,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":456.2832,"MH...Da.":1366.83506,"Theo..MH...Da.":1366.83384,"DeltaM..ppm.":0.89,"Deltam.z..Da.":0.0004,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":21.70242,"Average.Reporter.S.N":121,"Ion.Inject.Time..ms.":0.799,"RT..min.":91.7045,"First.Scan":45093,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_01.raw","File.ID":"F7","Abundance..126":2793789.83277,"Abundance..127N":3001417.18129,"Abundance..127C":3500135.24446,"Abundance..128N":3394583.08447,"Abundance..128C":2878389.78065,"Abundance..129N":2988979.84336,"Abundance..129C":3509529.23089,"Abundance..130N":2922751.83123,"Abundance..130C":3262587.42629,"Abundance..131":3089925.62983,"Ions.Score":28,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":0.01013,"Percolator.q.Value":0.0022,"Percolator.PEP":0.02103}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (N2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].lFVGNLPPDITEEEMr.[K]","Modifications":"N-Term(TMT6plex); R16(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q15233","Master.Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","Protein.Accessions":"Q15233","Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":1050.0498,"MH...Da.":2099.09233,"Theo..MH...Da.":2099.09198,"DeltaM..ppm.":0.17,"Deltam.z..Da.":0.00018,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":70.2972,"Average.Reporter.S.N":23,"Ion.Inject.Time..ms.":26.198,"RT..min.":177.1851,"First.Scan":91450,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_03.raw","File.ID":"F2","Abundance..126":7153.08075,"Abundance..127N":7763.43789,"Abundance..127C":7727.15925,"Abundance..128N":8021.40744,"Abundance..128C":6839.20233,"Abundance..129N":9480.44361,"Abundance..129C":9332.57731,"Abundance..130N":8991.30546,"Abundance..130C":8254.42176,"Abundance..131":8522.09076,"Ions.Score":2,"Identity.Strict":27,"Identity.Relaxed":20,"Expectation.Value":3.73983,"Percolator.q.Value":0.00221,"Percolator.PEP":0.02222}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (G2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].iMQLLDVPPGEDk.[G]","Modifications":"N-Term(TMT6plex); K13(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q9NSD9","Master.Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","Protein.Accessions":"Q9NSD9","Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":641.03754,"MH...Da.":1921.09806,"Theo..MH...Da.":1921.09601,"DeltaM..ppm.":1.07,"Deltam.z..Da.":0.00068,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":58.15062,"Average.Reporter.S.N":22.5,"Ion.Inject.Time..ms.":20.81,"RT..min.":176.0312,"First.Scan":89196,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_02.raw","File.ID":"F9","Abundance..126":21670.56993,"Abundance..127N":22442.87298,"Abundance..127C":18055.93751,"Abundance..128N":12498.9885,"Abundance..128C":19386.96665,"Abundance..129N":17896.5483,"Abundance..129C":24522.16448,"Abundance..130N":17741.35046,"Abundance..130C":19870.82101,"Abundance..131":20179.11616,"Ions.Score":6,"Identity.Strict":30,"Identity.Relaxed":23,"Expectation.Value":2.27073,"Percolator.q.Value":0.00222,"Percolator.PEP":0.02079}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (A2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].sPLAQMEEErr.[E]","Modifications":"N-Term(TMT6plex); R10(Label:13C(6)15N(4)); R11(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q16181","Master.Protein.Descriptions":"Septin-7 OS=Homo sapiens GN=SEPT7 PE=1 SV=2","Protein.Accessions":"Q16181","Protein.Descriptions":"Septin-7 OS=Homo sapiens GN=SEPT7 PE=1 SV=2","X..Missed.Cleavages":1,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":532.28235,"MH...Da.":1594.83249,"Theo..MH...Da.":1594.83234,"DeltaM..ppm.":0.09,"Deltam.z..Da.":0.00005,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":24.09375,"Average.Reporter.S.N":11.1,"Ion.Inject.Time..ms.":8.31,"RT..min.":76.252,"First.Scan":33630,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_01.raw","File.ID":"F15","Abundance..126":10110.13995,"Abundance..127N":8728.87776,"Abundance..127C":11231.80634,"Abundance..128N":11513.01213,"Abundance..128C":12993.32234,"Abundance..129N":9324.06079,"Abundance..129C":10340.0045,"Abundance..130N":9381.26943,"Abundance..130C":7167.13634,"Abundance..131":10007.92108,"Ions.Score":9,"Identity.Strict":27,"Identity.Relaxed":20,"Expectation.Value":0.75578,"Percolator.q.Value":0.00223,"Percolator.PEP":0.02373}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (N2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].wGDAGAEYVVESTGVFTTMEk.[A]","Modifications":"N-Term(TMT6plex); K21(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":915.13269,"MH...Da.":2743.38352,"Theo..MH...Da.":2743.37805,"DeltaM..ppm.":1.99,"Deltam.z..Da.":0.00182,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":22.75728,"Average.Reporter.S.N":3.8,"Ion.Inject.Time..ms.":50,"RT..min.":234.4607,"First.Scan":120777,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_03.raw","File.ID":"F2","Abundance..127N":1614.08633,"Abundance..127C":1856.39095,"Abundance..128N":1250.3537,"Abundance..128C":1532.11942,"Abundance..129N":1570.95504,"Abundance..129C":1189.08174,"Abundance..130N":986.82767,"Abundance..130C":1700.58228,"Abundance..131":1449.32143,"Ions.Score":5,"Identity.Strict":27,"Identity.Relaxed":20,"Expectation.Value":1.64196,"Percolator.q.Value":0.00223,"Percolator.PEP":0.02268}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (K2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].iPSAVGYQPTLATDMGTmQEr.[I]","Modifications":"N-Term(TMT6plex); M18(Oxidation); R21(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":841.08954,"MH...Da.":2521.25406,"Theo..MH...Da.":2521.25034,"DeltaM..ppm.":1.47,"Deltam.z..Da.":0.00124,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":0,"Average.Reporter.S.N":5.3,"Ion.Inject.Time..ms.":7.636,"RT..min.":153.7121,"First.Scan":80849,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_03.raw","File.ID":"F5","Abundance..126":5800.37739,"Abundance..127N":8200.90555,"Abundance..127C":5810.4598,"Abundance..128N":8264.81405,"Abundance..128C":7596.59614,"Abundance..129N":6689.38073,"Abundance..129C":8059.169,"Abundance..130N":6833.57062,"Abundance..130C":11382.52686,"Abundance..131":5097.08739,"Ions.Score":7,"Identity.Strict":25,"Identity.Relaxed":18,"Expectation.Value":0.65359,"Percolator.q.Value":0.00225,"Percolator.PEP":0.02398}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (H2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].nIFIEcTGTDFTk.[A]","Modifications":"N-Term(TMT6plex); C6(Carbamidomethyl); K13(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q9NSD9","Master.Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","Protein.Accessions":"Q9NSD9","Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":1006.53961,"MH...Da.":2012.07195,"Theo..MH...Da.":2012.06543,"DeltaM..ppm.":3.24,"Deltam.z..Da.":0.00326,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":29.71908,"Average.Reporter.S.N":11.3,"Ion.Inject.Time..ms.":29.807,"RT..min.":160.191,"First.Scan":80655,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_02.raw","File.ID":"F8","Abundance..126":3573.71013,"Abundance..127N":3846.0704,"Abundance..127C":4977.26622,"Abundance..128N":4728.281,"Abundance..128C":3862.27124,"Abundance..129N":2843.3799,"Abundance..129C":3801.68547,"Abundance..130N":3323.65458,"Abundance..130C":4299.87944,"Abundance..131":4389.19077,"Ions.Score":15,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.28254,"Percolator.q.Value":0.00226,"Percolator.PEP":0.02398}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (H2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[K].vLTPELYAELr.[A]","Modifications":"N-Term(TMT6plex); R11(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P12277","Master.Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","Protein.Accessions":"P12277","Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":0.9667,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":771.95276,"MH...Da.":1542.89824,"Theo..MH...Da.":1542.89683,"DeltaM..ppm.":0.91,"Deltam.z..Da.":0.00071,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":0,"Average.Reporter.S.N":18.5,"Ion.Inject.Time..ms.":15.049,"RT..min.":162.1905,"First.Scan":81816,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_02.raw","File.ID":"F8","Abundance..126":40130.59991,"Abundance..127N":45689.9334,"Abundance..127C":42935.02041,"Abundance..128N":51947.55514,"Abundance..128C":43572.24908,"Abundance..129N":42263.74844,"Abundance..129C":51789.81655,"Abundance..130N":37153.09039,"Abundance..130C":34177.84663,"Abundance..131":39622.58187,"Ions.Score":30,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.01013,"Percolator.q.Value":0.00226,"Percolator.PEP":0.02442}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (F2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].gFQQILAGEYDHLPEQAFYmVGPIEEAVAk.[A]","Modifications":"N-Term(TMT6plex); M20(Oxidation); K30(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":4,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":958.99921,"MH...Da.":3832.975,"Theo..MH...Da.":3832.97517,"DeltaM..ppm.":-0.04,"Deltam.z..Da.":-0.00004,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":6.48879,"Average.Reporter.S.N":4.4,"Ion.Inject.Time..ms.":30.418,"RT..min.":214.1179,"First.Scan":112517,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture4_02.raw","File.ID":"F10","Abundance..127N":2130.09804,"Abundance..127C":2358.35739,"Abundance..128N":2110.78957,"Abundance..128C":1366.1283,"Abundance..129N":1597.64753,"Abundance..129C":1820.9991,"Abundance..130N":1916.28087,"Abundance..130C":3026.27729,"Abundance..131":1806.47036,"Ions.Score":5,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":2.41538,"Percolator.q.Value":0.00228,"Percolator.PEP":0.01696}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (F2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].nIFIEcTGTDFTk.[A]","Modifications":"N-Term(TMT6plex); C6(Carbamidomethyl); K13(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q9NSD9","Master.Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","Protein.Accessions":"Q9NSD9","Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":1006.53632,"MH...Da.":2012.06536,"Theo..MH...Da.":2012.06543,"DeltaM..ppm.":-0.04,"Deltam.z..Da.":-0.00004,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":32.13673,"Average.Reporter.S.N":11.5,"Ion.Inject.Time..ms.":15.662,"RT..min.":160.3208,"First.Scan":82821,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture4_02.raw","File.ID":"F10","Abundance..126":3836.74139,"Abundance..127N":2817.60608,"Abundance..127C":3436.38595,"Abundance..128N":3506.97139,"Abundance..128C":3392.68404,"Abundance..129N":4857.98924,"Abundance..129C":4495.36968,"Abundance..130N":3909.12234,"Abundance..130C":3230.51105,"Abundance..131":5446.65228,"Ions.Score":12,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.47029,"Percolator.q.Value":0.0023,"Percolator.PEP":0.01748}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (F2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].sQVLDDEDSNNITVGSLVTVLVk.[L]","Modifications":"N-Term(TMT6plex); K23(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q9UGP8","Master.Protein.Descriptions":"Translocation protein SEC63 homolog OS=Homo sapiens GN=SEC63 PE=1 SV=2","Protein.Accessions":"Q9UGP8","Protein.Descriptions":"Translocation protein SEC63 homolog OS=Homo sapiens GN=SEC63 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":971.20825,"MH...Da.":2911.6102,"Theo..MH...Da.":2911.61195,"DeltaM..ppm.":-0.6,"Deltam.z..Da.":-0.00058,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":17.85124,"Average.Reporter.S.N":6.8,"Ion.Inject.Time..ms.":15.476,"RT..min.":201.0213,"First.Scan":105791,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture4_02.raw","File.ID":"F10","Abundance..126":4149.41052,"Abundance..127N":5879.82155,"Abundance..127C":3728.6767,"Abundance..128N":4900.26255,"Abundance..128C":4209.2587,"Abundance..129N":1997.3088,"Abundance..129C":4950.86367,"Abundance..130N":5179.60771,"Abundance..130C":4778.67926,"Abundance..131":5512.2936,"Ions.Score":1,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":6.81195,"Percolator.q.Value":0.0023,"Percolator.PEP":0.01746}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (H2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].hEHQVMLmr.[Q]","Modifications":"N-Term(TMT6plex); M8(Oxidation); R9(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q15233","Master.Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","Protein.Accessions":"Q15233","Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.9,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":479.2515,"MH...Da.":1435.73993,"Theo..MH...Da.":1435.73751,"DeltaM..ppm.":1.69,"Deltam.z..Da.":0.00081,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":80.05074,"Average.Reporter.S.N":4,"Ion.Inject.Time..ms.":50,"RT..min.":25.502,"First.Scan":8368,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_02.raw","File.ID":"F8","Abundance..126":1716.47879,"Abundance..127N":1276.70263,"Abundance..127C":1291.90498,"Abundance..128N":1253.12557,"Abundance..128C":1811.225,"Abundance..129N":934.03644,"Abundance..129C":1559.16955,"Abundance..130N":1387.67997,"Abundance..130C":888.03805,"Abundance..131":1223.29327,"Ions.Score":10,"Identity.Strict":25,"Identity.Relaxed":18,"Expectation.Value":0.41379,"Percolator.q.Value":0.00231,"Percolator.PEP":0.02501}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (B2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].vIISAPSADAPMFVmGVNHEk.[Y]","Modifications":"N-Term(TMT6plex); M15(Oxidation); K21(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":2,"m.z..Da.":899.15363,"MH...Da.":2695.44632,"Theo..MH...Da.":2695.4443,"DeltaM..ppm.":0.75,"Deltam.z..Da.":0.00067,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":59.13591,"Average.Reporter.S.N":5.6,"Ion.Inject.Time..ms.":16.382,"RT..min.":160.6875,"First.Scan":84728,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_01.raw","File.ID":"F14","Abundance..126":11267.63467,"Abundance..127N":8945.26526,"Abundance..127C":6475.17289,"Abundance..128N":12256.20857,"Abundance..128C":14323.31616,"Abundance..129N":6834.80109,"Abundance..129C":10597.97013,"Abundance..130N":11750.73255,"Abundance..130C":8419.00856,"Abundance..131":13020.84509,"Ions.Score":0,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":7.96928,"Percolator.q.Value":0.00233,"Percolator.PEP":0.02393}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (O2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].sQVLDDEDSNNITVGSLVTVLVk.[L]","Modifications":"N-Term(TMT6plex); K23(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q9UGP8","Master.Protein.Descriptions":"Translocation protein SEC63 homolog OS=Homo sapiens GN=SEC63 PE=1 SV=2","Protein.Accessions":"Q9UGP8","Protein.Descriptions":"Translocation protein SEC63 homolog OS=Homo sapiens GN=SEC63 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.9091,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":971.20972,"MH...Da.":2911.6146,"Theo..MH...Da.":2911.61195,"DeltaM..ppm.":0.91,"Deltam.z..Da.":0.00088,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":55.66509,"Average.Reporter.S.N":11.9,"Ion.Inject.Time..ms.":11.271,"RT..min.":200.9041,"First.Scan":106708,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_03.raw","File.ID":"F1","Abundance..126":6119.13692,"Abundance..127N":6254.69364,"Abundance..127C":6795.96922,"Abundance..128N":6975.62795,"Abundance..128C":7514.01112,"Abundance..129N":8434.2824,"Abundance..129C":8729.13388,"Abundance..130N":8511.45969,"Abundance..130C":6912.70983,"Abundance..131":6574.92386,"Ions.Score":11,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.75759,"Percolator.q.Value":0.00262,"Percolator.PEP":0.02812}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (B2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[K].dLFDPIIEDr.[H]","Modifications":"N-Term(TMT6plex); R10(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P12277","Master.Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","Protein.Accessions":"P12277","Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":0.9583,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":736.39978,"MH...Da.":1471.79228,"Theo..MH...Da.":1471.78694,"DeltaM..ppm.":3.63,"Deltam.z..Da.":0.00267,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":43.04208,"Average.Reporter.S.N":16.7,"Ion.Inject.Time..ms.":7.002,"RT..min.":169.445,"First.Scan":89848,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_01.raw","File.ID":"F14","Abundance..126":22104.22082,"Abundance..127N":18200.08079,"Abundance..127C":25187.59358,"Abundance..128N":22304.84048,"Abundance..128C":20688.45995,"Abundance..129N":21281.84053,"Abundance..129C":23899.01839,"Abundance..130N":21746.05719,"Abundance..130C":26463.01704,"Abundance..131":18915.31842,"Ions.Score":48,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":0.00011,"Percolator.q.Value":0.00267,"Percolator.PEP":0.0295}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (J2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[K].vLTPELYAELr.[A]","Modifications":"N-Term(TMT6plex); R11(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P12277","Master.Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","Protein.Accessions":"P12277","Protein.Descriptions":"Creatine kinase B-type OS=Homo sapiens GN=CKB PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":0.9688,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":771.95288,"MH...Da.":1542.89849,"Theo..MH...Da.":1542.89683,"DeltaM..ppm.":1.07,"Deltam.z..Da.":0.00083,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":45.27208,"Average.Reporter.S.N":20.2,"Ion.Inject.Time..ms.":4.021,"RT..min.":162.2008,"First.Scan":85166,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture4_03.raw","File.ID":"F6","Abundance..126":109874.85151,"Abundance..127N":115643.28226,"Abundance..127C":128914.3401,"Abundance..128N":124700.6727,"Abundance..128C":109599.29714,"Abundance..129N":122906.28661,"Abundance..129C":118906.50179,"Abundance..130N":120598.46949,"Abundance..130C":136347.82935,"Abundance..131":117657.15788,"Ions.Score":32,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.00618,"Percolator.q.Value":0.00268,"Percolator.PEP":0.02873}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (O2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].qLELrQEEEr.[R]","Modifications":"N-Term(TMT6plex); R5(Label:13C(6)15N(4)); R10(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q15233","Master.Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","Protein.Accessions":"Q15233","Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","X..Missed.Cleavages":1,"Charge":3,"DeltaScore":0.625,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":526.95642,"MH...Da.":1578.85471,"Theo..MH...Da.":1578.85519,"DeltaM..ppm.":-0.3,"Deltam.z..Da.":-0.00016,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":22.07229,"Average.Reporter.S.N":29.7,"Ion.Inject.Time..ms.":12.609,"RT..min.":57.4505,"First.Scan":24382,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_03.raw","File.ID":"F1","Abundance..126":56609.24778,"Abundance..127N":47033.61884,"Abundance..127C":53612.57128,"Abundance..128N":44012.27635,"Abundance..128C":46921.3264,"Abundance..129N":51354.42163,"Abundance..129C":60531.83523,"Abundance..130N":57610.19052,"Abundance..130C":53389.84546,"Abundance..131":50919.58983,"Ions.Score":16,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.2045,"Percolator.q.Value":0.00285,"Percolator.PEP":0.03041}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (N2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].qLELrQEEEr.[R]","Modifications":"N-Term(TMT6plex); R5(Label:13C(6)15N(4)); R10(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q15233","Master.Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","Protein.Accessions":"Q15233","Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","X..Missed.Cleavages":1,"Charge":3,"DeltaScore":0.4,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":526.95703,"MH...Da.":1578.85654,"Theo..MH...Da.":1578.85519,"DeltaM..ppm.":0.86,"Deltam.z..Da.":0.00045,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":20.9263,"Average.Reporter.S.N":36.6,"Ion.Inject.Time..ms.":14.772,"RT..min.":57.2799,"First.Scan":23816,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_03.raw","File.ID":"F2","Abundance..126":22836.26837,"Abundance..127N":21768.57005,"Abundance..127C":23551.34488,"Abundance..128N":22812.02073,"Abundance..128C":24857.25985,"Abundance..129N":24189.91793,"Abundance..129C":30437.7888,"Abundance..130N":20646.54706,"Abundance..130C":22176.2328,"Abundance..131":22552.55944,"Ions.Score":10,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.84716,"Percolator.q.Value":0.0032,"Percolator.PEP":0.03953}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (H2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].eVLGDAVPDEILIEAVLk.[N]","Modifications":"N-Term(TMT6plex); K18(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q9Y450","Master.Protein.Descriptions":"HBS1-like protein OS=Homo sapiens GN=HBS1L PE=1 SV=1","Protein.Accessions":"Q9Y450","Protein.Descriptions":"HBS1-like protein OS=Homo sapiens GN=HBS1L PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.2857,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":797.14172,"MH...Da.":2389.41062,"Theo..MH...Da.":2389.40855,"DeltaM..ppm.":0.87,"Deltam.z..Da.":0.00069,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":16.27298,"Average.Reporter.S.N":1.7,"Ion.Inject.Time..ms.":50,"RT..min.":219.0613,"First.Scan":111523,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_02.raw","File.ID":"F8","Abundance..126":1337.06564,"Abundance..127C":359.02637,"Abundance..128C":453.56135,"Abundance..129N":1450.68563,"Abundance..129C":1404.90687,"Abundance..131":1056.62561,"Ions.Score":7,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":1.63716,"Percolator.q.Value":0.0032,"Percolator.PEP":0.03695}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (M2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].wGDAGAEYVVESTGVFTTMEk.[A]","Modifications":"N-Term(TMT6plex); K21(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":915.13177,"MH...Da.":2743.38077,"Theo..MH...Da.":2743.37805,"DeltaM..ppm.":0.99,"Deltam.z..Da.":0.00091,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":81.69665,"Ion.Inject.Time..ms.":50,"RT..min.":263.2321,"First.Scan":130230,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_03.raw","File.ID":"F3","Ions.Score":4,"Identity.Strict":27,"Identity.Relaxed":20,"Expectation.Value":2.3291,"Percolator.q.Value":0.00415,"Percolator.PEP":0.05684}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (M2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[R].eGNDLYHEMIESGVINLk.[D]","Modifications":"N-Term(TMT6plex); K18(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":4,"DeltaScore":0,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":632.58997,"MH...Da.":2527.33803,"Theo..MH...Da.":2527.33579,"DeltaM..ppm.":0.89,"Deltam.z..Da.":0.00056,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":30.2319,"Average.Reporter.S.N":27.3,"Ion.Inject.Time..ms.":2.178,"RT..min.":173.3547,"First.Scan":87861,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_03.raw","File.ID":"F3","Abundance..126":93643.27447,"Abundance..127N":74845.17576,"Abundance..127C":83732.59528,"Abundance..128N":90665.88119,"Abundance..128C":84604.91283,"Abundance..129N":86512.47125,"Abundance..129C":100324.92829,"Abundance..130N":86306.6359,"Abundance..130C":88863.76835,"Abundance..131":110155.97406,"Ions.Score":3,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":3.97607,"Percolator.q.Value":0.00434,"Percolator.PEP":0.05969}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (N2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].hEHQVMLmr.[Q]","Modifications":"N-Term(TMT6plex); M8(Oxidation); R9(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q15233","Master.Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","Protein.Accessions":"Q15233","Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","X..Missed.Cleavages":0,"Charge":4,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":359.68982,"MH...Da.":1435.73745,"Theo..MH...Da.":1435.73751,"DeltaM..ppm.":-0.04,"Deltam.z..Da.":-0.00002,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":10.12212,"Average.Reporter.S.N":7.2,"Ion.Inject.Time..ms.":3.608,"RT..min.":20.7983,"First.Scan":6023,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_03.raw","File.ID":"F2","Abundance..126":5556.6211,"Abundance..127N":7738.37046,"Abundance..127C":4767.93758,"Abundance..128N":7294.20743,"Abundance..128C":5755.73131,"Abundance..129N":4956.42806,"Abundance..129C":6964.31751,"Abundance..130N":6987.67305,"Abundance..130C":6171.06201,"Abundance..131":2616.07044,"Ions.Score":5,"Identity.Strict":25,"Identity.Relaxed":18,"Expectation.Value":1.17004,"Percolator.q.Value":0.00435,"Percolator.PEP":0.06301}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (O2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].vALVYGQmNEPPGAr.[A]","Modifications":"N-Term(TMT6plex); M8(Oxidation); R15(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":928.98975,"MH...Da.":1856.97222,"Theo..MH...Da.":1856.97655,"DeltaM..ppm.":-2.34,"Deltam.z..Da.":-0.00217,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":0,"Average.Reporter.S.N":14.1,"Ion.Inject.Time..ms.":50,"RT..min.":117.0561,"First.Scan":58338,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_03.raw","File.ID":"F1","Abundance..126":4514.67945,"Abundance..127N":4353.19881,"Abundance..127C":5931.42072,"Abundance..128N":4382.79541,"Abundance..128C":4882.85515,"Abundance..129N":4107.14224,"Abundance..129C":4930.45919,"Abundance..130N":5242.28567,"Abundance..130C":4341.52404,"Abundance..131":4902.21317,"Ions.Score":20,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":0.07374,"Percolator.q.Value":0.00566,"Percolator.PEP":0.0832}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (O2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[R].hEHQVMLmr.[Q]","Modifications":"N-Term(TMT6plex); M8(Oxidation); R9(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q15233","Master.Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","Protein.Accessions":"Q15233","Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","X..Missed.Cleavages":0,"Charge":4,"DeltaScore":0.375,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":359.68967,"MH...Da.":1435.73684,"Theo..MH...Da.":1435.73751,"DeltaM..ppm.":-0.47,"Deltam.z..Da.":-0.00017,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":41.28204,"Average.Reporter.S.N":22.8,"Ion.Inject.Time..ms.":37.75,"RT..min.":30.1571,"First.Scan":10479,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_03.raw","File.ID":"F1","Abundance..126":8091.45729,"Abundance..127N":6737.33363,"Abundance..127C":6170.2704,"Abundance..128N":8393.45377,"Abundance..128C":8846.71839,"Abundance..129N":7041.45599,"Abundance..129C":9339.33756,"Abundance..130N":8482.0777,"Abundance..130C":7763.81535,"Abundance..131":7204.68972,"Ions.Score":8,"Identity.Strict":25,"Identity.Relaxed":18,"Expectation.Value":0.6244,"Percolator.q.Value":0.0057,"Percolator.PEP":0.08444}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (F2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[R].iMQLLDVPPGEDk.[G]","Modifications":"N-Term(TMT6plex); K13(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q9NSD9","Master.Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","Protein.Accessions":"Q9NSD9","Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaCn":0,"Rank":2,"Search.Engine.Rank":2,"m.z..Da.":641.0376,"MH...Da.":1921.09824,"Theo..MH...Da.":1921.09601,"DeltaM..ppm.":1.16,"Deltam.z..Da.":0.00074,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":0,"Average.Reporter.S.N":51,"Ion.Inject.Time..ms.":11.812,"RT..min.":176.5347,"First.Scan":92091,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture4_02.raw","File.ID":"F10","Abundance..126":44618.808,"Abundance..127N":46954.57317,"Abundance..127C":36858.57339,"Abundance..128N":46827.21701,"Abundance..128C":44757.07049,"Abundance..129N":64483.59986,"Abundance..129C":45876.06631,"Abundance..130N":49220.7907,"Abundance..130C":52746.40734,"Abundance..131":56713.34515,"Ions.Score":1,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":7.7772,"Percolator.q.Value":0.00574,"Percolator.PEP":0.08363}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (C2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].kMPLPLk.[L]","Modifications":"N-Term(TMT6plex); K1(Label); K7(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q9NSD9","Master.Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","Protein.Accessions":"Q9NSD9","Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","X..Missed.Cleavages":1,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":510.68488,"MH...Da.":1530.04007,"Theo..MH...Da.":1530.0391,"DeltaM..ppm.":0.64,"Deltam.z..Da.":0.00032,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":54.29869,"Average.Reporter.S.N":56.6,"Ion.Inject.Time..ms.":14.131,"RT..min.":116.9262,"First.Scan":57835,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture4_01.raw","File.ID":"F13","Abundance..126":40644.26385,"Abundance..127N":43077.36839,"Abundance..127C":31366.01426,"Abundance..128N":35747.3242,"Abundance..128C":34806.52709,"Abundance..129N":35354.85218,"Abundance..129C":36270.15967,"Abundance..130N":33647.81624,"Abundance..130C":32847.03939,"Abundance..131":39765.80182,"Ions.Score":6,"Identity.Strict":25,"Identity.Relaxed":18,"Expectation.Value":0.8512,"Percolator.q.Value":0.00576,"Percolator.PEP":0.08888}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (C2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[R].vLLLSHLAr.[M]","Modifications":"N-Term(TMT6plex); R9(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q9UGP8","Master.Protein.Descriptions":"Translocation protein SEC63 homolog OS=Homo sapiens GN=SEC63 PE=1 SV=2","Protein.Accessions":"Q9UGP8","Protein.Descriptions":"Translocation protein SEC63 homolog OS=Homo sapiens GN=SEC63 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":3,"DeltaCn":0,"Rank":2,"Search.Engine.Rank":2,"m.z..Da.":420.9458,"MH...Da.":1260.82285,"Theo..MH...Da.":1260.82288,"DeltaM..ppm.":-0.02,"Deltam.z..Da.":-1e-05,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":37.6062,"Average.Reporter.S.N":13.2,"Ion.Inject.Time..ms.":50,"RT..min.":138.0731,"First.Scan":69655,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture4_01.raw","File.ID":"F13","Abundance..126":3759.22898,"Abundance..127N":4833.17832,"Abundance..127C":3741.33997,"Abundance..128N":5863.17696,"Abundance..128C":4244.76639,"Abundance..129N":4943.48045,"Abundance..129C":4493.47774,"Abundance..130N":4362.10686,"Abundance..130C":4957.02611,"Abundance..131":3932.86458,"Ions.Score":8,"Identity.Strict":23,"Identity.Relaxed":16,"Expectation.Value":0.39736,"Percolator.q.Value":0.00576,"Percolator.PEP":0.0894}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (G2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[K].vIHDNFGIVEGLmTTVHAITATQk.[T]","Modifications":"N-Term(TMT6plex); M13(Oxidation); K24(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":0,"Charge":5,"DeltaScore":0.75,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":616.34534,"MH...Da.":3077.69758,"Theo..MH...Da.":3077.69491,"DeltaM..ppm.":0.87,"Deltam.z..Da.":0.00053,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":11.35086,"Average.Reporter.S.N":7.2,"Ion.Inject.Time..ms.":3.924,"RT..min.":175.5927,"First.Scan":88945,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_02.raw","File.ID":"F9","Abundance..126":23725.35514,"Abundance..127N":15044.15812,"Abundance..127C":17228.52768,"Abundance..128N":35980.50268,"Abundance..128C":21800.26367,"Abundance..129N":19965.93241,"Abundance..129C":23320.81054,"Abundance..130N":20915.9424,"Abundance..130C":22985.07213,"Abundance..131":26184.37699,"Ions.Score":4,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":3.4367,"Percolator.q.Value":0.00577,"Percolator.PEP":0.07921}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (K2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].sLQDIIAILGmDELSEEDk.[L]","Modifications":"N-Term(TMT6plex); M11(Oxidation); K19(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":867.802,"MH...Da.":2601.39145,"Theo..MH...Da.":2601.38247,"DeltaM..ppm.":3.45,"Deltam.z..Da.":0.00299,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":25.15707,"Average.Reporter.S.N":10.2,"Ion.Inject.Time..ms.":50,"RT..min.":236.0564,"First.Scan":125754,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_03.raw","File.ID":"F5","Abundance..126":3266.28666,"Abundance..127N":4107.76732,"Abundance..127C":3173.41343,"Abundance..128N":3042.84916,"Abundance..128C":3504.49305,"Abundance..129N":3171.40697,"Abundance..129C":4211.23294,"Abundance..130N":3853.8837,"Abundance..130C":2016.87973,"Abundance..131":3651.61334,"Ions.Score":1,"Identity.Strict":30,"Identity.Relaxed":23,"Expectation.Value":8.365,"Percolator.q.Value":0.00593,"Percolator.PEP":0.0913}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (K2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].hEHQVMLmr.[Q]","Modifications":"N-Term(TMT6plex); M8(Oxidation); R9(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q15233","Master.Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","Protein.Accessions":"Q15233","Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":479.25116,"MH...Da.":1435.73893,"Theo..MH...Da.":1435.73751,"DeltaM..ppm.":0.99,"Deltam.z..Da.":0.00047,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":44.53422,"Average.Reporter.S.N":3.4,"Ion.Inject.Time..ms.":50,"RT..min.":24.8281,"First.Scan":7856,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_03.raw","File.ID":"F5","Abundance..126":1198.7609,"Abundance..127N":1074.84708,"Abundance..127C":1716.3601,"Abundance..128N":1263.64475,"Abundance..128C":1101.01794,"Abundance..129N":1143.04654,"Abundance..129C":815.42648,"Abundance..130N":1349.47873,"Abundance..130C":1325.40149,"Abundance..131":492.32238,"Ions.Score":4,"Identity.Strict":25,"Identity.Relaxed":18,"Expectation.Value":1.457,"Percolator.q.Value":0.00595,"Percolator.PEP":0.09215}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (N2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].rVIISAPSADAPmFVMGVNHEk.[Y]","Modifications":"N-Term(TMT6plex); R1(Label:13C(6)15N(4)); M13(Oxidation); K22(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":1,"Charge":4,"DeltaScore":0.5,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":716.14514,"MH...Da.":2861.55874,"Theo..MH...Da.":2861.55368,"DeltaM..ppm.":1.77,"Deltam.z..Da.":0.00126,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":4.21102,"Average.Reporter.S.N":12.6,"Ion.Inject.Time..ms.":0.187,"RT..min.":131.7285,"First.Scan":65369,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_03.raw","File.ID":"F2","Abundance..126":168780.026,"Abundance..127N":119436.50225,"Abundance..127C":190617.44649,"Abundance..128N":151163.93895,"Abundance..128C":174107.07177,"Abundance..129N":157824.63224,"Abundance..129C":112562.55103,"Abundance..130N":178094.8808,"Abundance..130C":136296.26568,"Abundance..131":145149.10827,"Ions.Score":14,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.37052,"Percolator.q.Value":0.00596,"Percolator.PEP":0.1039}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (G2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].iPVGPETLGr.[I]","Modifications":"N-Term(TMT6plex); R10(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":639.38641,"MH...Da.":1277.76555,"Theo..MH...Da.":1277.76542,"DeltaM..ppm.":0.1,"Deltam.z..Da.":0.00006,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":37.73961,"Average.Reporter.S.N":17.5,"Ion.Inject.Time..ms.":0.578,"RT..min.":126.0844,"First.Scan":60885,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_02.raw","File.ID":"F9","Abundance..126":243765.20106,"Abundance..127N":269975.29367,"Abundance..127C":298221.18509,"Abundance..128N":310674.22643,"Abundance..128C":205695.87387,"Abundance..129N":322799.49282,"Abundance..129C":280472.23309,"Abundance..130N":291698.48232,"Abundance..130C":283277.82226,"Abundance..131":259514.91522,"Ions.Score":9,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":0.90593,"Percolator.q.Value":0.00626,"Percolator.PEP":0.08905}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (H2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].tIAMDGTEGLVr.[G]","Modifications":"N-Term(TMT6plex); R12(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":0.92,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":751.40253,"MH...Da.":1501.79778,"Theo..MH...Da.":1501.81211,"DeltaM..ppm.":-9.55,"Deltam.z..Da.":-0.00717,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":44.76791,"Average.Reporter.S.N":28.6,"Ion.Inject.Time..ms.":10.19,"RT..min.":125.6536,"First.Scan":61076,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_02.raw","File.ID":"F8","Abundance..126":39224.52853,"Abundance..127N":36766.12216,"Abundance..127C":42570.52556,"Abundance..128N":37101.20896,"Abundance..128C":41502.40235,"Abundance..129N":38595.96571,"Abundance..129C":45901.87656,"Abundance..130N":40418.0623,"Abundance..130C":30588.39648,"Abundance..131":42866.46369,"Ions.Score":25,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":0.02245,"Percolator.q.Value":0.00635,"Percolator.PEP":0.1042}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (L2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].eYTAcELMNIYk.[T]","Modifications":"N-Term(TMT6plex); C5(Carbamidomethyl); K12(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q9NSD9","Master.Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","Protein.Accessions":"Q9NSD9","Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.75,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":667.68292,"MH...Da.":2001.03421,"Theo..MH...Da.":2001.03169,"DeltaM..ppm.":1.26,"Deltam.z..Da.":0.00084,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":37.7364,"Average.Reporter.S.N":67.9,"Ion.Inject.Time..ms.":5.687,"RT..min.":163.0306,"First.Scan":82507,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_02.raw","File.ID":"F4","Abundance..126":105375.28069,"Abundance..127N":93217.48157,"Abundance..127C":109700.47373,"Abundance..128N":98417.0858,"Abundance..128C":88380.80858,"Abundance..129N":102868.84055,"Abundance..129C":104122.22062,"Abundance..130N":98398.27086,"Abundance..130C":114842.72023,"Abundance..131":86926.69977,"Ions.Score":12,"Identity.Strict":27,"Identity.Relaxed":20,"Expectation.Value":0.40101,"Percolator.q.Value":0.00644,"Percolator.PEP":0.1083}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (L2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].hEHQVmLmr.[Q]","Modifications":"N-Term(TMT6plex); M6(Oxidation); M8(Oxidation); R9(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q15233","Master.Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","Protein.Accessions":"Q15233","Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","X..Missed.Cleavages":0,"Charge":4,"DeltaScore":0.8333,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":363.68829,"MH...Da.":1451.73134,"Theo..MH...Da.":1451.73242,"DeltaM..ppm.":-0.74,"Deltam.z..Da.":-0.00027,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":50.23343,"Average.Reporter.S.N":13.8,"Ion.Inject.Time..ms.":50,"RT..min.":24.4634,"First.Scan":7710,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_02.raw","File.ID":"F4","Abundance..126":6389.73585,"Abundance..127N":4783.91537,"Abundance..127C":4570.65157,"Abundance..128N":4759.60991,"Abundance..128C":5345.64922,"Abundance..129N":4030.00734,"Abundance..129C":3770.00618,"Abundance..130N":4482.90154,"Abundance..130C":4533.725,"Abundance..131":3836.66515,"Ions.Score":6,"Identity.Strict":24,"Identity.Relaxed":17,"Expectation.Value":0.73841,"Percolator.q.Value":0.00658,"Percolator.PEP":0.1105}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (K2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].eNFSLDWck.[Q]","Modifications":"N-Term(TMT6plex); C8(Carbamidomethyl); K9(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P23919","Master.Protein.Descriptions":"Thymidylate kinase OS=Homo sapiens GN=DTYMK PE=1 SV=4","Protein.Accessions":"P23919","Protein.Descriptions":"Thymidylate kinase OS=Homo sapiens GN=DTYMK PE=1 SV=4","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":832.93237,"MH...Da.":1664.85747,"Theo..MH...Da.":1664.8598,"DeltaM..ppm.":-1.4,"Deltam.z..Da.":-0.00116,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":50.7551,"Average.Reporter.S.N":13.7,"Ion.Inject.Time..ms.":4.033,"RT..min.":155.3575,"First.Scan":81829,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_03.raw","File.ID":"F5","Abundance..126":50143.83012,"Abundance..127N":60747.6403,"Abundance..127C":54969.74915,"Abundance..128N":38960.10172,"Abundance..128C":36145.02069,"Abundance..129N":47543.5524,"Abundance..129C":52937.65929,"Abundance..130N":50123.47207,"Abundance..130C":46055.06076,"Abundance..131":53267.18914,"Ions.Score":7,"Identity.Strict":27,"Identity.Relaxed":20,"Expectation.Value":1.08984,"Percolator.q.Value":0.00661,"Percolator.PEP":0.1059}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (O2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[K].vIPELNGk.[L]","Modifications":"N-Term(TMT6plex); K8(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.4286,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":445.95444,"MH...Da.":1335.84876,"Theo..MH...Da.":1335.84916,"DeltaM..ppm.":-0.3,"Deltam.z..Da.":-0.00013,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":23.50163,"Average.Reporter.S.N":100.2,"Ion.Inject.Time..ms.":4.101,"RT..min.":129.9323,"First.Scan":65874,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture1_03.raw","File.ID":"F1","Abundance..126":476849.68885,"Abundance..127N":413518.82981,"Abundance..127C":461563.19392,"Abundance..128N":520489.42837,"Abundance..128C":488329.48876,"Abundance..129N":468581.13197,"Abundance..129C":464037.3694,"Abundance..130N":471371.83901,"Abundance..130C":418556.49601,"Abundance..131":450291.40983,"Ions.Score":14,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.35765,"Percolator.q.Value":0.00664,"Percolator.PEP":0.1016}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (D2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].hEHQVmLmr.[Q]","Modifications":"N-Term(TMT6plex); M6(Oxidation); M8(Oxidation); R9(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q15233","Master.Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","Protein.Accessions":"Q15233","Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","X..Missed.Cleavages":0,"Charge":4,"DeltaScore":0.6667,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":363.68842,"MH...Da.":1451.73183,"Theo..MH...Da.":1451.73242,"DeltaM..ppm.":-0.41,"Deltam.z..Da.":-0.00015,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":79.72844,"Average.Reporter.S.N":7.3,"Ion.Inject.Time..ms.":50,"RT..min.":21.4037,"First.Scan":6275,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_02.raw","File.ID":"F12","Abundance..126":3164.85758,"Abundance..127N":2501.72784,"Abundance..127C":2436.44721,"Abundance..128N":2649.22875,"Abundance..128C":1875.18243,"Abundance..129N":3413.9882,"Abundance..129C":1968.60221,"Abundance..130N":3294.70834,"Abundance..130C":2220.07751,"Abundance..131":2383.35298,"Ions.Score":6,"Identity.Strict":24,"Identity.Relaxed":17,"Expectation.Value":0.82661,"Percolator.q.Value":0.00732,"Percolator.PEP":0.1125}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (G2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].vVDLmAHMASkE.[-]","Modifications":"N-Term(TMT6plex); M5(Oxidation); K11(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":1,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":605.00073,"MH...Da.":1812.98764,"Theo..MH...Da.":1812.98435,"DeltaM..ppm.":1.82,"Deltam.z..Da.":0.0011,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":31.61334,"Average.Reporter.S.N":41.1,"Ion.Inject.Time..ms.":5.262,"RT..min.":109.652,"First.Scan":51757,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_02.raw","File.ID":"F9","Abundance..126":120784.68421,"Abundance..127N":121980.75576,"Abundance..127C":122260.6565,"Abundance..128N":105389.67543,"Abundance..128C":121581.64021,"Abundance..129N":126424.34299,"Abundance..129C":106745.9156,"Abundance..130N":125474.10848,"Abundance..130C":124864.93254,"Abundance..131":116941.51739,"Ions.Score":7,"Identity.Strict":30,"Identity.Relaxed":23,"Expectation.Value":2.21474,"Percolator.q.Value":0.00738,"Percolator.PEP":0.1166}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (D4)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].tVLIMELINNVAk.[A]","Modifications":"N-Term(TMT6plex); K13(TMT6plex)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":0.7895,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":639.39441,"MH...Da.":1916.16867,"Theo..MH...Da.":1916.16548,"DeltaM..ppm.":1.67,"Deltam.z..Da.":0.00107,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":13.26052,"Average.Reporter.S.N":49.2,"Ion.Inject.Time..ms.":28.001,"RT..min.":209.5808,"First.Scan":111532,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_02.raw","File.ID":"F12","Abundance..126":32476.6899,"Abundance..127N":38443.69476,"Abundance..127C":31330.76395,"Abundance..128N":35694.28392,"Abundance..128C":35118.56915,"Abundance..129N":34665.58827,"Abundance..129C":38695.8391,"Abundance..130N":31791.85665,"Abundance..130C":38274.76749,"Abundance..131":32250.46425,"Ions.Score":19,"Identity.Strict":32,"Identity.Relaxed":25,"Expectation.Value":0.2157,"Percolator.q.Value":0.00806,"Percolator.PEP":0.1451}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (G2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].sLQDIIAILGmDELSEEDk.[L]","Modifications":"N-Term(TMT6plex); M11(Oxidation); K19(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":4,"DeltaScore":0.9231,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":651.10596,"MH...Da.":2601.402,"Theo..MH...Da.":2601.38247,"DeltaM..ppm.":7.51,"Deltam.z..Da.":0.00488,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":0,"Average.Reporter.S.N":13.4,"Ion.Inject.Time..ms.":25.417,"RT..min.":200.4732,"First.Scan":102250,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_02.raw","File.ID":"F9","Abundance..126":5394.96099,"Abundance..127N":6448.8614,"Abundance..127C":7883.13865,"Abundance..128N":5382.71657,"Abundance..128C":7596.32681,"Abundance..129N":5685.68817,"Abundance..129C":6396.24781,"Abundance..130N":6726.20493,"Abundance..130C":5910.09083,"Abundance..131":4444.9536,"Ions.Score":13,"Identity.Strict":30,"Identity.Relaxed":23,"Expectation.Value":0.61996,"Percolator.q.Value":0.00807,"Percolator.PEP":0.1421}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (L2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].pLGELWk.[-]","Modifications":"N-Term(TMT6plex); K7(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P23919","Master.Protein.Descriptions":"Thymidylate kinase OS=Homo sapiens GN=DTYMK PE=1 SV=4","Protein.Accessions":"P23919","Protein.Descriptions":"Thymidylate kinase OS=Homo sapiens GN=DTYMK PE=1 SV=4","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":0.0588,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":654.91272,"MH...Da.":1308.81816,"Theo..MH...Da.":1308.81713,"DeltaM..ppm.":0.79,"Deltam.z..Da.":0.00052,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":36.42314,"Average.Reporter.S.N":34.1,"Ion.Inject.Time..ms.":4.878,"RT..min.":156.6629,"First.Scan":78842,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture5_02.raw","File.ID":"F4","Abundance..126":138315.76867,"Abundance..127N":124993.09475,"Abundance..127C":135315.05366,"Abundance..128N":117293.39191,"Abundance..128C":146053.53934,"Abundance..129N":100373.20549,"Abundance..129C":128264.74646,"Abundance..130N":138144.09234,"Abundance..130C":113813.38627,"Abundance..131":118143.10042,"Ions.Score":17,"Identity.Strict":27,"Identity.Relaxed":20,"Expectation.Value":0.10868,"Percolator.q.Value":0.00808,"Percolator.PEP":0.1392}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (B2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[K].vDIVAINDPFIDLNYMVYmFQYDSTHGk.[F]","Modifications":"N-Term(TMT6plex); M19(Oxidation); K28(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P04406","Master.Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","Protein.Accessions":"P04406","Protein.Descriptions":"Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3","X..Missed.Cleavages":0,"Charge":4,"DeltaScore":0.4615,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":948.48785,"MH...Da.":3790.92959,"Theo..MH...Da.":3790.89923,"DeltaM..ppm.":8.01,"Deltam.z..Da.":0.00759,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":6.60049,"Average.Reporter.S.N":7.4,"Ion.Inject.Time..ms.":36.051,"RT..min.":210.5024,"First.Scan":112481,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture3_01.raw","File.ID":"F14","Abundance..126":2170.15509,"Abundance..127N":2607.1092,"Abundance..127C":3646.40594,"Abundance..128N":3348.2404,"Abundance..128C":2763.39546,"Abundance..129N":3370.23971,"Abundance..129C":2584.41619,"Abundance..130N":2511.28569,"Abundance..130C":3300.65388,"Abundance..131":2192.51323,"Ions.Score":13,"Identity.Strict":27,"Identity.Relaxed":20,"Expectation.Value":0.29252,"Percolator.q.Value":0.00814,"Percolator.PEP":0.1479}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (M2)","PSM.Ambiguity":"Selected","Annotated.Sequence":"[R].eTPENLAk.[L]","Modifications":"N-Term(TMT6plex); K8(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q9NSD9","Master.Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","Protein.Accessions":"Q9NSD9","Protein.Descriptions":"Phenylalanine--tRNA ligase beta subunit OS=Homo sapiens GN=FARSB PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":0.6897,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":684.40558,"MH...Da.":1367.80388,"Theo..MH...Da.":1367.8026,"DeltaM..ppm.":0.94,"Deltam.z..Da.":0.00064,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":15.2027,"Average.Reporter.S.N":25.1,"Ion.Inject.Time..ms.":18.204,"RT..min.":82.3899,"First.Scan":36802,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_03.raw","File.ID":"F3","Abundance..126":18985.3658,"Abundance..127N":18867.21444,"Abundance..127C":17404.04457,"Abundance..128N":22538.88475,"Abundance..128C":16577.46589,"Abundance..129N":18571.47518,"Abundance..129C":20590.23962,"Abundance..130N":23920.43344,"Abundance..130C":21455.64692,"Abundance..131":21406.80143,"Ions.Score":29,"Identity.Strict":29,"Identity.Relaxed":22,"Expectation.Value":0.01267,"Percolator.q.Value":0.0092,"Percolator.PEP":0.1729}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (M2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[R].gAFGHEr.[Y]","Modifications":"N-Term(TMT6plex); R7(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P23919","Master.Protein.Descriptions":"Thymidylate kinase OS=Homo sapiens GN=DTYMK PE=1 SV=4","Protein.Accessions":"P23919","Protein.Descriptions":"Thymidylate kinase OS=Homo sapiens GN=DTYMK PE=1 SV=4","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":338.18457,"MH...Da.":1012.53916,"Theo..MH...Da.":1012.54011,"DeltaM..ppm.":-0.94,"Deltam.z..Da.":-0.00032,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":45.85233,"Average.Reporter.S.N":2.7,"Ion.Inject.Time..ms.":50,"RT..min.":17.8471,"First.Scan":4782,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_03.raw","File.ID":"F3","Abundance..126":1102.11291,"Abundance..127N":1107.41492,"Abundance..128N":471.07245,"Abundance..128C":1313.72144,"Abundance..129N":1381.25784,"Abundance..129C":873.60758,"Abundance..130N":973.84233,"Abundance..130C":1002.27906,"Abundance..131":1201.92983,"Ions.Score":9,"Identity.Strict":18,"Identity.Relaxed":13,"Expectation.Value":0.083,"Percolator.q.Value":0.0092,"Percolator.PEP":0.1723}
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{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (F2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].lEmEMEAAr.[H]","Modifications":"N-Term(TMT6plex); M3(Oxidation); R9(Label:13C(6)15N(4))","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"Q15233","Master.Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","Protein.Accessions":"Q15233","Protein.Descriptions":"Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4","X..Missed.Cleavages":0,"Charge":2,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":667.8299,"MH...Da.":1334.65251,"Theo..MH...Da.":1334.65211,"DeltaM..ppm.":0.3,"Deltam.z..Da.":0.0002,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":46.99839,"Average.Reporter.S.N":9.5,"Ion.Inject.Time..ms.":7.765,"RT..min.":92.0033,"First.Scan":43713,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture4_02.raw","File.ID":"F10","Abundance..126":9037.28283,"Abundance..127N":11114.57155,"Abundance..127C":6713.51784,"Abundance..128N":7122.80574,"Abundance..128C":9014.50209,"Abundance..129N":8178.81282,"Abundance..129C":10808.68425,"Abundance..130N":8976.86449,"Abundance..130C":9406.68387,"Abundance..131":9819.25852,"Ions.Score":5,"Identity.Strict":23,"Identity.Relaxed":16,"Expectation.Value":0.88499,"Percolator.q.Value":0.00987,"Percolator.PEP":0.2028}
{"Checked":false,"Confidence":"High","Identifying.Node":"Mascot (A2)","PSM.Ambiguity":"Unambiguous","Annotated.Sequence":"[K].gFQQILAGEYDHLPEQAFYmVGPIEEAVAk.[A]","Modifications":"N-Term(TMT6plex); M20(Oxidation); K30(Label)","X..Protein.Groups":1,"X..Proteins":1,"Master.Protein.Accessions":"P06576","Master.Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","Protein.Accessions":"P06576","Protein.Descriptions":"ATP synthase subunit beta, mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"DeltaScore":1,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"m.z..Da.":1278.33215,"MH...Da.":3832.98191,"Theo..MH...Da.":3832.97517,"DeltaM..ppm.":1.76,"Deltam.z..Da.":0.00225,"Activation.Type":"CID","MS.Order":"MS2","Isolation.Interference....":0,"Average.Reporter.S.N":3.8,"Ion.Inject.Time..ms.":50,"RT..min.":215.6236,"First.Scan":111141,"Spectrum.File":"161117_SILAC_HeLa_UPS1_TMT10_Mixture2_01.raw","File.ID":"F15","Abundance..126":1890.51341,"Abundance..127N":1029.28206,"Abundance..128N":1089.97648,"Abundance..128C":1052.61879,"Abundance..129N":1616.23606,"Abundance..129C":2343.78751,"Abundance..130N":1386.07015,"Abundance..130C":1313.94376,"Abundance..131":1423.09016,"Ions.Score":6,"Identity.Strict":28,"Identity.Relaxed":21,"Expectation.Value":1.86347,"Percolator.q.Value":0.00996,"Percolator.PEP":0.1795}
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