{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"AAEAGETGAATSATEGDNNNNTAAGDKK","Annotated.Sequence":"[K].AAEAGETGAATSATEGDNNNNTAAGDKK.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.734,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":879.39395,"Contaminant":"False","Aligned.m.z..Da.":879.39395,"MH...Da.":2636.1673,"Theo..MH...Da.":2636.16665,"DeltaM..ppm.":0.25,"Deltam.z..Da.":0.00022,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":7769382,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":1.32109,"Ion.Inject.Time..ms.":22,"RT..min.":4.9337,"First.Scan":1851,"Last.Scan":1851,"Master.Scan.s.":1848,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":3606,"XCorr":8.57,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":6.30512e-16,"Percolator.SVMScore":9.86951,"Precursor.Area":5859362,"Apex.RT..min.":4.94,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LEAGLSDSK","Annotated.Sequence":"[K].LEAGLsDSK.[Q]","Modifications":"S6(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1038,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":500.22367,"Contaminant":"False","Aligned.m.z..Da.":500.22367,"MH...Da.":999.44007,"Theo..MH...Da.":999.43943,"DeltaM..ppm.":0.64,"Deltam.z..Da.":0.00032,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2978492.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":25.54549,"Ion.Inject.Time..ms.":22,"RT..min.":7.5681,"First.Scan":5317,"Last.Scan":5317,"Master.Scan.s.":5306,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":679,"XCorr":2.6,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":0.00037,"Percolator.SVMScore":1.135,"Precursor.Area":5397380.5,"Apex.RT..min.":7.58,"ptmRS..Binomial.Peptide.Score":308.92,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(8): 788.38; y1+-H(3) O(4) P(7): 659.34; y1+-H(3) O(4) P(6): 588.30; y1+-H(3) O(4) P(5): 531.28; y1+-H(3) O(4) P(4): 418.19; y2+-H(3) O(4) P(6): 294.65; y1+(3): 349.17; y1+(2): 234.14","ptmRS..Best.Site.Probabilities":"S6(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(6): 100.0; S(8): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LQETNPEEVPK","Annotated.Sequence":"[K].LQETNPEEVPK.[F]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.4962,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":642.32846,"Contaminant":"False","Aligned.m.z..Da.":642.32846,"MH...Da.":1283.64965,"Theo..MH...Da.":1283.64777,"DeltaM..ppm.":1.47,"Deltam.z..Da.":0.00094,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2776155.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":10.36353,"Ion.Inject.Time..ms.":22,"RT..min.":7.5687,"First.Scan":5318,"Last.Scan":5318,"Master.Scan.s.":5306,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":511,"XCorr":2.62,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":0.00001,"Percolator.SVMScore":1.719,"Precursor.Area":66213752,"Apex.RT..min.":7.61,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LREENFSSNTSELGNKK","Annotated.Sequence":"[K].LREENFSSNtSELGNKK.[H]","Modifications":"T10(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":2,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.1122,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":678.31738,"Contaminant":"False","Aligned.m.z..Da.":678.31738,"MH...Da.":2032.9376,"Theo..MH...Da.":2032.93354,"DeltaM..ppm.":2,"Deltam.z..Da.":0.00135,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2369740.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":6.09863,"Ion.Inject.Time..ms.":22,"RT..min.":7.572,"First.Scan":5320,"Last.Scan":5320,"Master.Scan.s.":5319,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":1894,"XCorr":5.26,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":3.015e-07,"Percolator.SVMScore":2.428,"ptmRS..Binomial.Peptide.Score":439.9,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(10): 1160.53; b1+-H(3) O(4) P(12): 1376.61; y1+-H(3) O(4) P(10): 1059.54; y1+-H(3) O(4) P(9): 972.51; y1+-H(3) O(4) P(8): 858.47; y2+-H(3) O(4) P(10): 530.28; y2+-H(3) O(4) P(9): 486.76; y2+-H(3) O(4) P(8): 429.74; y1+(7): 775.43; y2+(7): 388.22","ptmRS..Best.Site.Probabilities":"T10(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(7): 0.0; S(8): 0.0; T(10): 100.0; S(11): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LLDNTNTDVK","Annotated.Sequence":"[K].LLDNTNTDVK.[I]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q08421","Master.Protein.Descriptions":"Enhancer of translation termination 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ETT1 PE=1 SV=1","Protein.Accessions":"Q08421","Protein.Descriptions":"Enhancer of translation termination 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ETT1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.548,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":566.79618,"Contaminant":"False","Aligned.m.z..Da.":566.79618,"MH...Da.":1132.58509,"Theo..MH...Da.":1132.58444,"DeltaM..ppm.":0.57,"Deltam.z..Da.":0.00032,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2483459,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":24.0516,"Ion.Inject.Time..ms.":22,"RT..min.":7.5726,"First.Scan":5321,"Last.Scan":5321,"Master.Scan.s.":5319,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":505,"XCorr":2.5,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":0.00007,"Percolator.SVMScore":1.428,"Precursor.Area":1715639.125,"Apex.RT..min.":7.56,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"HSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[R].HSDSYsENETNHTNVPISSTGGTNNK.[T]","Modifications":"S6(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0091,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":957.4013,"Contaminant":"False","Aligned.m.z..Da.":957.4013,"MH...Da.":2870.18934,"Theo..MH...Da.":2870.18608,"DeltaM..ppm.":1.14,"Deltam.z..Da.":0.00109,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":4993433,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":7.5738,"First.Scan":5323,"Last.Scan":5323,"Master.Scan.s.":5319,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":2549,"XCorr":7.66,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":6.116e-14,"Percolator.SVMScore":5.227,"Precursor.Area":22296560,"Apex.RT..min.":7.59,"ptmRS..Binomial.Peptide.Score":611.48,"ptmRS..Isoform.Confidence.Probability":0.006,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(4): 409.15; b1+-H(3) O(4) P(5): 572.21; b1+-H(3) O(4) P(6): 659.24; b1+-H(3) O(4) P(9): 1031.37; b1+-H(3) O(4) P(10): 1132.42; b1+-H(3) O(4) P(11): 1246.46; b1+-H(3) O(4) P(12): 1383.52; b1+-H(3) O(4) P(14): 1598.61; b1+-H(3) O(4) P(15): 1697.68; b2+-H(3) O(4) P(9): 516.19; b2+-H(3) O(4) P(12): 692.26; b2+-H(3) O(4) P(13): 742.79; b2+-H(3) O(4) P(14): 799.81; b2+-H(3) O(4) P(15): 849.34; y2+-H(3) O(4) P(25): 1318.08; y2+-H(3) O(4) P(24): 1274.56; y2+-H(3) O(4) P(23): 1217.05","ptmRS..Best.Site.Probabilities":"S4(Phospho): 99.4","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(4): 99.4; Y(5): 0.0; S(6): 0.6; T(10): 0.0; T(13): 0.0; S(18): 0.0; S(19): 0.0; T(20): 0.0; T(23): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"HSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[R].HSDSySENETNHTNVPISSTGGTNNK.[T]","Modifications":"Y5(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":0,"Charge":4,"Original.Precursor.Charge":4,"DeltaScore":0.0064,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":718.30239,"Contaminant":"False","Aligned.m.z..Da.":718.30239,"MH...Da.":2870.18774,"Theo..MH...Da.":2870.18608,"DeltaM..ppm.":0.58,"Deltam.z..Da.":0.00042,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2482961,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":10.54103,"Ion.Inject.Time..ms.":22,"RT..min.":7.5745,"First.Scan":5324,"Last.Scan":5324,"Master.Scan.s.":5319,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":2495,"XCorr":6.25,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":2.078e-09,"Percolator.SVMScore":3.332,"Precursor.Area":10104933,"Apex.RT..min.":7.59,"ptmRS..Binomial.Peptide.Score":292.13,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(9): 1031.37; b1+-H(3) O(4) P(10): 1132.42; b1+-H(3) O(4) P(11): 1246.46; b1+-H(3) O(4) P(12): 1383.52; b2+-H(3) O(4) P(7): 394.65; b2+-H(3) O(4) P(8): 451.67; b2+-H(3) O(4) P(9): 516.19; b2+-H(3) O(4) P(13): 742.79; b2+-H(3) O(4) P(14): 799.81; b2+-H(3) O(4) P(15): 849.34; b2+-H(3) O(4) P(17): 954.41; b1+(4): 427.16","ptmRS..Best.Site.Probabilities":"S6(Phospho): 99.34","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(4): 0.7; Y(5): 0.0; S(6): 99.3; T(10): 0.0; T(13): 0.0; S(18): 0.0; S(19): 0.0; T(20): 0.0; T(23): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].mSEGItDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); T6(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1555,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1096.47018,"Contaminant":"False","Aligned.m.z..Da.":1096.47018,"MH...Da.":2191.93308,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":2.39,"Deltam.z..Da.":0.00262,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1697517.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":16.52426,"Ion.Inject.Time..ms.":22,"RT..min.":15.4805,"First.Scan":15606,"Last.Scan":15606,"Master.Scan.s.":15602,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":2179,"XCorr":5.66,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":3.234e-13,"Percolator.SVMScore":4.925,"Precursor.Area":1568161.625,"Apex.RT..min.":15.49,"ptmRS..Binomial.Peptide.Score":571.23,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(6): 512.24; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+-H(3) O(4) P(9): 869.39; b1+-H(3) O(4) P(10): 998.43; b1+-H(3) O(4) P(13): 1326.61; y1+-H(3) O(4) P(14): 1665.76; y2+-H(3) O(4) P(14): 833.38; b1+(2): 130.05; b1+(3): 259.09; b1+(4): 316.11; b1+(5): 429.20; b2+(3): 130.05","ptmRS..Best.Site.Probabilities":"T6(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; T(6): 100.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"TAAFVIPTLEK","Annotated.Sequence":"[K].TAAFVIPTLEK.[V]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P39517","Master.Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","Protein.Accessions":"P39517","Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6879,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":595.34484,"Contaminant":"False","Aligned.m.z..Da.":595.34484,"MH...Da.":1189.68241,"Theo..MH...Da.":1189.6827,"DeltaM..ppm.":-0.25,"Deltam.z..Da.":-0.00015,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2428782.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":15.4793,"First.Scan":15604,"Last.Scan":15604,"Master.Scan.s.":15602,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":204,"XCorr":2.98,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":0.00007,"Percolator.SVMScore":1.432,"Precursor.Area":1928554.375,"Apex.RT..min.":15.47,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"IQDPQMTGYVSTR","Annotated.Sequence":"[R].IQDPQmTGyVSTR.[Y]","Modifications":"M6(Oxidation); Y9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P32485","Master.Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","Protein.Accessions":"P32485","Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1303,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":796.34499,"Contaminant":"False","Aligned.m.z..Da.":796.34499,"MH...Da.":1591.6827,"Theo..MH...Da.":1591.6822,"DeltaM..ppm.":0.31,"Deltam.z..Da.":0.00025,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2458314,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":7.5775,"First.Scan":5329,"Last.Scan":5329,"Master.Scan.s.":5319,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":1236,"XCorr":3.07,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":1.097e-06,"Percolator.SVMScore":2.194,"Precursor.Area":12172759,"Apex.RT..min.":7.63,"ptmRS..Binomial.Peptide.Score":268.62,"ptmRS..Isoform.Confidence.Probability":0.333,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(11): 1252.56; y1+-H(3) O(4) P(10): 1137.54","ptmRS..Best.Site.Probabilities":"T7(Phospho): 33.28; Y9(Phospho): 33.28; S11(Phospho): 33.28","ptmRS..Phospho.Site.Probabilities":"T(7): 33.3; Y(9): 33.3; S(11): 33.3; T(12): 0.2"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"IGNNELLHR","Annotated.Sequence":"[K].IGNNELLHR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q03558","Master.Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","Protein.Accessions":"Q03558","Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.6832,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":355.86473,"Contaminant":"False","Aligned.m.z..Da.":355.86473,"MH...Da.":1065.57964,"Theo..MH...Da.":1065.57997,"DeltaM..ppm.":-0.31,"Deltam.z..Da.":-0.00011,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":7841962.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":1.26214,"Ion.Inject.Time..ms.":15.595,"RT..min.":7.5831,"First.Scan":5335,"Last.Scan":5335,"Master.Scan.s.":5332,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":300,"XCorr":3.03,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":0.0002,"Percolator.SVMScore":1.249,"Precursor.Area":12870501,"Apex.RT..min.":7.6,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"IGNNELLHR","Annotated.Sequence":"[K].IGNNELLHR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q03558","Master.Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","Protein.Accessions":"Q03558","Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.698,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":533.29377,"Contaminant":"False","Aligned.m.z..Da.":533.29377,"MH...Da.":1065.58026,"Theo..MH...Da.":1065.57997,"DeltaM..ppm.":0.28,"Deltam.z..Da.":0.00015,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3489722.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":44.06003,"Ion.Inject.Time..ms.":22,"RT..min.":7.585,"First.Scan":5338,"Last.Scan":5338,"Master.Scan.s.":5332,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":300,"XCorr":2.98,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":0.00003,"Percolator.SVMScore":1.613,"Precursor.Area":6625931.5,"Apex.RT..min.":7.6,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].mSEGItDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); T6(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1242,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1096.47201,"Contaminant":"False","Aligned.m.z..Da.":1096.47201,"MH...Da.":2191.93673,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":4.06,"Deltam.z..Da.":0.00445,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1835722.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":61.34652,"Ion.Inject.Time..ms.":22,"RT..min.":15.4893,"First.Scan":15617,"Last.Scan":15617,"Master.Scan.s.":15615,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":2166,"XCorr":4.43,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":4.721e-10,"Percolator.SVMScore":3.601,"Precursor.Area":1568161.625,"Apex.RT..min.":15.49,"ptmRS..Binomial.Peptide.Score":554.81,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(6): 512.24; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+-H(3) O(4) P(9): 869.39; b1+-H(3) O(4) P(10): 998.43; b1+-H(3) O(4) P(12): 1213.52; b1+-H(3) O(4) P(13): 1326.61; y1+-H(3) O(4) P(14): 1665.76; y2+-H(3) O(4) P(16): 918.44; b1+(3): 259.09; b1+(4): 316.11; b1+(5): 429.20","ptmRS..Best.Site.Probabilities":"T6(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; T(6): 100.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].mSEGItDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); T6(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.1781,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":731.3137,"Contaminant":"False","Aligned.m.z..Da.":731.3137,"MH...Da.":2191.92654,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":-0.59,"Deltam.z..Da.":-0.00043,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1617514.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":27.97613,"Ion.Inject.Time..ms.":22,"RT..min.":15.4705,"First.Scan":15593,"Last.Scan":15593,"Master.Scan.s.":15589,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":1954,"XCorr":4.38,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":6.743e-08,"Percolator.SVMScore":2.7,"Precursor.Area":1540606.125,"Apex.RT..min.":15.49,"ptmRS..Binomial.Peptide.Score":405.44,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(6): 512.24; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+-H(3) O(4) P(9): 869.39; b1+-H(3) O(4) P(10): 998.43; b1+(2): 130.05; b1+(3): 259.09; b1+(4): 316.11; b1+(5): 429.20; b2+(3): 130.05","ptmRS..Best.Site.Probabilities":"T6(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; T(6): 100.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"IQDPQMTGYVSTR","Annotated.Sequence":"[R].IQDPQmtGYVsTR.[Y]","Modifications":"M6(Oxidation); T7(Phospho); S11(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P32485","Master.Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","Protein.Accessions":"P32485","Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.0453,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":836.3282,"Contaminant":"False","Aligned.m.z..Da.":836.3282,"MH...Da.":1671.64912,"Theo..MH...Da.":1671.64853,"DeltaM..ppm.":0.35,"Deltam.z..Da.":0.0003,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":4278635,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":39.46001,"Ion.Inject.Time..ms.":22,"RT..min.":7.6743,"First.Scan":5455,"Last.Scan":5455,"Master.Scan.s.":5449,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":764,"XCorr":3.09,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":0.00004,"Percolator.SVMScore":1.54,"Precursor.Area":7242883.5,"Apex.RT..min.":7.7,"ptmRS..Binomial.Peptide.Score":366.12,"ptmRS..Isoform.Confidence.Probability":0.005,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(11): 1332.53; y1+-H(3) O(4) P(10): 1217.50; y1+-H(3) O(4) P(8): 992.39; y1+-H(3) O(4) P(7): 845.36; y2+-H(3) O(4) P(11): 666.77; y2+-H(3) O(4) P(10): 609.25; y1+(4): 462.27; y1+(3): 363.20","ptmRS..Best.Site.Probabilities":"T7(Phospho): 100; Y9(Phospho): 99.48","ptmRS..Phospho.Site.Probabilities":"T(7): 100.0; Y(9): 99.5; S(11): 0.5; T(12): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSSPLHRLSPTGK","Annotated.Sequence":"[R].mSsPLHRLsPTGK.[S]","Modifications":"M1(Oxidation); S3(Phospho); S9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.4545,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":529.565,"Contaminant":"False","Aligned.m.z..Da.":529.565,"MH...Da.":1586.68044,"Theo..MH...Da.":1586.67977,"DeltaM..ppm.":0.42,"Deltam.z..Da.":0.00022,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3781914,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":12.66541,"Ion.Inject.Time..ms.":22,"RT..min.":7.6755,"First.Scan":5457,"Last.Scan":5457,"Master.Scan.s.":5449,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":979,"XCorr":3.96,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":0.00013,"Percolator.SVMScore":1.325,"Precursor.Area":17156138,"Apex.RT..min.":7.71,"ptmRS..Binomial.Peptide.Score":253.78,"ptmRS..Isoform.Confidence.Probability":0.992,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(8): 877.50; y1+-H(3) O(4) P(7): 740.44; y2+-H(3) O(4) P(12): 671.34; y2+-H(3) O(4) P(10): 544.32; y2+-H(3) O(4) P(9): 495.80; y2+-H(3) O(4) P(8): 439.25; y1+(4): 402.23","ptmRS..Best.Site.Probabilities":"S3(Phospho): 99.99; S9(Phospho): 99.2","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(3): 100.0; S(9): 99.2; T(11): 0.8"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSSPLHRLSPTGK","Annotated.Sequence":"[R].mSsPLHRLsPTGK.[S]","Modifications":"M1(Oxidation); S3(Phospho); S9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.4819,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":793.84355,"Contaminant":"False","Aligned.m.z..Da.":793.84355,"MH...Da.":1586.67983,"Theo..MH...Da.":1586.67977,"DeltaM..ppm.":0.04,"Deltam.z..Da.":0.00003,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":4331488,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":30.15601,"Ion.Inject.Time..ms.":22,"RT..min.":7.6966,"First.Scan":5485,"Last.Scan":5485,"Master.Scan.s.":5475,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":975,"XCorr":2.49,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":0.00013,"Percolator.SVMScore":1.321,"Precursor.Area":5832718.5,"Apex.RT..min.":7.71,"ptmRS..Binomial.Peptide.Score":325.28,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(3): 304.10; b1+-H(3) O(4) P(8): 920.48; y2+-H(3) O(4) P(11): 627.82; y2+- -2H(3) O(4) P -2H(3) O(4) P(11): 578.83; b1+(2): 235.07","ptmRS..Best.Site.Probabilities":"S3(Phospho): 100; S9(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(3): 100.0; S(9): 100.0; T(11): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FFISNPDLVDRLEK","Annotated.Sequence":"[R].FFISNPDLVDRLEK.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q03558","Master.Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","Protein.Accessions":"Q03558","Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.8085,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":564.96956,"Contaminant":"False","Aligned.m.z..Da.":564.96956,"MH...Da.":1692.89413,"Theo..MH...Da.":1692.89555,"DeltaM..ppm.":-0.83,"Deltam.z..Da.":-0.00047,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2017525.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":18.4847,"Ion.Inject.Time..ms.":22,"RT..min.":15.3685,"First.Scan":15462,"Last.Scan":15462,"Master.Scan.s.":15459,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":530,"XCorr":4.02,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":1.231e-08,"Percolator.SVMScore":3.009,"Precursor.Area":3196542.25,"Apex.RT..min.":15.39,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEySDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); Y12(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.3611,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40449,"Contaminant":"False","Aligned.m.z..Da.":967.40449,"MH...Da.":2900.19891,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":-0.14,"Deltam.z..Da.":-0.00014,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1903609.875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":24.53546,"Ion.Inject.Time..ms.":22,"RT..min.":15.8247,"First.Scan":16050,"Last.Scan":16050,"Master.Scan.s.":16044,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":2487,"XCorr":4.57,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":4.475e-07,"Percolator.SVMScore":2.356,"Precursor.Area":1032483.3125,"Apex.RT..min.":16.12,"ptmRS..Binomial.Peptide.Score":320.23,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(14): 1528.61; y1+-H(3) O(4) P(15): 1750.78; b2+-H(3) O(4) P(18): 1014.90; b1+(12): 1344.56","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEySDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); Y12(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0021,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40864,"Contaminant":"False","Aligned.m.z..Da.":967.40864,"MH...Da.":2900.21137,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":4.15,"Deltam.z..Da.":0.00402,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1594641,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":28.91236,"Ion.Inject.Time..ms.":22,"RT..min.":15.8015,"First.Scan":16019,"Last.Scan":16019,"Master.Scan.s.":16018,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":2657,"XCorr":4.8,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":9.605e-09,"Percolator.SVMScore":3.054,"ptmRS..Binomial.Peptide.Score":304.08,"ptmRS..Isoform.Confidence.Probability":0.009,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(14): 1528.61","ptmRS..Best.Site.Probabilities":"S13(Phospho): 99.08","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.9; S(13): 99.1; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"VELGLSHFPESQFLK","Annotated.Sequence":"[R].VELGLSHFPESQFLK.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q08421","Master.Protein.Descriptions":"Enhancer of translation termination 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ETT1 PE=1 SV=1","Protein.Accessions":"Q08421","Protein.Descriptions":"Enhancer of translation termination 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ETT1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.5654,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":577.64117,"Contaminant":"False","Aligned.m.z..Da.":577.64117,"MH...Da.":1730.90896,"Theo..MH...Da.":1730.9112,"DeltaM..ppm.":-1.29,"Deltam.z..Da.":-0.00075,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1005015.3125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":45.66441,"Ion.Inject.Time..ms.":22,"RT..min.":15.7682,"First.Scan":15978,"Last.Scan":15978,"Master.Scan.s.":15966,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":625,"XCorr":2.6,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":1.175e-07,"Percolator.SVMScore":2.599,"Precursor.Area":658997.875,"Apex.RT..min.":15.76,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].msEGITDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S2(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.166,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1096.46739,"Contaminant":"False","Aligned.m.z..Da.":1096.46739,"MH...Da.":2191.9275,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":-0.16,"Deltam.z..Da.":-0.00017,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":879338.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":81.21954,"Ion.Inject.Time..ms.":22,"RT..min.":15.8851,"First.Scan":16128,"Last.Scan":16128,"Master.Scan.s.":16122,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":1955,"XCorr":2.53,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":0.00002,"Percolator.SVMScore":1.671,"Precursor.Area":46310836,"Apex.RT..min.":15.73,"ptmRS..Binomial.Peptide.Score":207.93,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(2): 112.04; b1+-H(3) O(4) P(3): 241.08; b1+-H(3) O(4) P(4): 298.10; b1+-H(3) O(4) P(5): 411.19; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35","ptmRS..Best.Site.Probabilities":"S2(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 100.0; T(6): 0.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"QLSEDSHHLQSNNDNFSK","Annotated.Sequence":"[K].QLSEDSHHLQSNNDNFsK.[K]","Modifications":"S17(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.1347,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":727.30689,"Contaminant":"False","Aligned.m.z..Da.":727.30689,"MH...Da.":2179.90612,"Theo..MH...Da.":2179.90403,"DeltaM..ppm.":0.96,"Deltam.z..Da.":0.0007,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3186417,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":8.89325,"Ion.Inject.Time..ms.":22,"RT..min.":6.895,"First.Scan":4431,"Last.Scan":4431,"Master.Scan.s.":4422,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":1873,"XCorr":4.01,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":0.00034,"Percolator.SVMScore":1.149,"ptmRS..Binomial.Peptide.Score":236.46,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(11): 1285.59; y1+-H(3) O(4) P(10): 1148.53; y1+-H(3) O(4) P(9): 1035.45; y1+-H(3) O(4) P(8): 907.39; y1+-H(3) O(4) P(4): 477.25; y1+-H(3) O(4) P(3): 363.20; y1+-H(3) O(4) P(2): 216.13; y2+-H(3) O(4) P(16): 920.90; y2+-H(3) O(4) P(11): 643.30; y1+(2): 314.11","ptmRS..Best.Site.Probabilities":"S17(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(3): 0.0; S(6): 0.0; S(11): 0.0; S(17): 100.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":2,"DeltaScore":0.0335,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.4044,"Contaminant":"False","Aligned.m.z..Da.":967.4044,"MH...Da.":2900.19865,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":-0.23,"Deltam.z..Da.":-0.00022,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1301401.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":52.34619,"Ion.Inject.Time..ms.":22,"RT..min.":15.6503,"First.Scan":15824,"Last.Scan":15824,"Master.Scan.s.":15823,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":2444,"XCorr":3.58,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":1.5e-07,"Percolator.SVMScore":2.555,"ptmRS..Binomial.Peptide.Score":269.44,"ptmRS..Isoform.Confidence.Probability":0.5,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"Y12(Phospho): 50; S13(Phospho): 50","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 50.0; S(13): 50.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ILISTDLLAR","Annotated.Sequence":"[R].ILISTDLLAR.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5114,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":557.84565,"Contaminant":"False","Aligned.m.z..Da.":557.84565,"MH...Da.":1114.68402,"Theo..MH...Da.":1114.68304,"DeltaM..ppm.":0.88,"Deltam.z..Da.":0.00049,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1880452.625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":21.49652,"Ion.Inject.Time..ms.":22,"RT..min.":15.5917,"First.Scan":15749,"Last.Scan":15749,"Master.Scan.s.":15745,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":124,"XCorr":2.64,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":0.00002,"Percolator.SVMScore":1.678,"Precursor.Area":16739197,"Apex.RT..min.":15.62,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ILISTDLLAR","Annotated.Sequence":"[R].ILISTDLLAR.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.4947,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":557.84558,"Contaminant":"False","Aligned.m.z..Da.":557.84558,"MH...Da.":1114.68389,"Theo..MH...Da.":1114.68304,"DeltaM..ppm.":0.77,"Deltam.z..Da.":0.00043,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":5627377,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":18.92561,"Ion.Inject.Time..ms.":20.5,"RT..min.":15.6017,"First.Scan":15762,"Last.Scan":15762,"Master.Scan.s.":15758,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":124,"XCorr":2.81,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":0.00013,"Percolator.SVMScore":1.323,"Precursor.Area":16739197,"Apex.RT..min.":15.62,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"NHQLEEEIEK","Annotated.Sequence":"[K].NHQLEEEIEK.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5607,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":634.80939,"Contaminant":"False","Aligned.m.z..Da.":634.80939,"MH...Da.":1268.61151,"Theo..MH...Da.":1268.61172,"DeltaM..ppm.":-0.17,"Deltam.z..Da.":-0.00011,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3940318.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":30.98143,"Ion.Inject.Time..ms.":22,"RT..min.":7.1901,"First.Scan":4819,"Last.Scan":4819,"Master.Scan.s.":4812,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":894,"XCorr":3.21,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":5.332e-07,"Percolator.SVMScore":2.324,"Precursor.Area":5166238,"Apex.RT..min.":7.2,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].msEGITDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S2(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":3,"DeltaScore":0.1272,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1096.46988,"Contaminant":"False","Aligned.m.z..Da.":1096.46988,"MH...Da.":2191.93248,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":2.11,"Deltam.z..Da.":0.00232,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3867909.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":46.66029,"Ion.Inject.Time..ms.":22,"RT..min.":15.6962,"First.Scan":15885,"Last.Scan":15885,"Master.Scan.s.":15875,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":2110,"XCorr":5.03,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":8.706e-10,"Percolator.SVMScore":3.49,"Precursor.Area":46310836,"Apex.RT..min.":15.73,"ptmRS..Binomial.Peptide.Score":560.4,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(3): 241.08; b1+-H(3) O(4) P(4): 298.10; b1+-H(3) O(4) P(5): 411.19; b1+-H(3) O(4) P(6): 512.24; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+-H(3) O(4) P(9): 869.39; b1+-H(3) O(4) P(10): 998.43; b1+-H(3) O(4) P(12): 1213.52; b1+-H(3) O(4) P(13): 1326.61; b1+(4): 396.08; b1+(5): 509.16; y1+(14): 1683.77","ptmRS..Best.Site.Probabilities":"S2(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 100.0; T(6): 0.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0449,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.4079,"Contaminant":"False","Aligned.m.z..Da.":967.4079,"MH...Da.":2900.20916,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":3.39,"Deltam.z..Da.":0.00328,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":5306562.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":48.07467,"Ion.Inject.Time..ms.":22,"RT..min.":15.2967,"First.Scan":15369,"Last.Scan":15369,"Master.Scan.s.":15368,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":2631,"XCorr":5.57,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":2.465e-08,"Percolator.SVMScore":2.883,"ptmRS..Binomial.Peptide.Score":449.45,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(13): 1413.58; b1+-H(3) O(4) P(16): 1771.69; y1+-H(3) O(4) P(15): 1750.78; y1+-H(3) O(4) P(14): 1621.73; y2+-H(3) O(4) P(14): 811.37; b1+(12): 1344.56","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FTVSAIYSDLPQQER","Annotated.Sequence":"[K].FTVSAIYSDLPQQER.[D]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.6352,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":585.2956,"Contaminant":"False","Aligned.m.z..Da.":585.2956,"MH...Da.":1753.87226,"Theo..MH...Da.":1753.87554,"DeltaM..ppm.":-1.87,"Deltam.z..Da.":-0.00109,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":843708.3125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":44.36479,"Ion.Inject.Time..ms.":22,"RT..min.":15.0194,"First.Scan":15015,"Last.Scan":15015,"Master.Scan.s.":15004,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":1156,"XCorr":3.07,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":0.00012,"Percolator.SVMScore":1.345,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"NQQLEDEIEK","Annotated.Sequence":"[K].NQQLEDEIEK.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.516,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":623.30195,"Contaminant":"False","Aligned.m.z..Da.":623.30195,"MH...Da.":1245.59663,"Theo..MH...Da.":1245.59574,"DeltaM..ppm.":0.72,"Deltam.z..Da.":0.00045,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":4874828,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":77.73094,"Ion.Inject.Time..ms.":6.787,"RT..min.":8.5645,"First.Scan":6626,"Last.Scan":6626,"Master.Scan.s.":6619,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":580,"XCorr":2.19,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":0.00002,"Percolator.SVMScore":1.703,"Precursor.Area":9229713,"Apex.RT..min.":8.58,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FTVSAIYSDLPQQER","Annotated.Sequence":"[K].FTVSAIYSDLPQQER.[D]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6733,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":877.44185,"Contaminant":"False","Aligned.m.z..Da.":877.44185,"MH...Da.":1753.87643,"Theo..MH...Da.":1753.87554,"DeltaM..ppm.":0.51,"Deltam.z..Da.":0.00044,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1964019.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":15.76201,"Ion.Inject.Time..ms.":22,"RT..min.":15.0151,"First.Scan":15008,"Last.Scan":15008,"Master.Scan.s.":15004,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":1072,"XCorr":4.04,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":2.669e-11,"Percolator.SVMScore":4.123,"Precursor.Area":9093708,"Apex.RT..min.":15.04,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSSPLHRLSPTGK","Annotated.Sequence":"[R].MSsPLHRLsPTGK.[S]","Modifications":"S3(Phospho); S9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.5224,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":524.23248,"Contaminant":"False","Aligned.m.z..Da.":524.23248,"MH...Da.":1570.6829,"Theo..MH...Da.":1570.68486,"DeltaM..ppm.":-1.25,"Deltam.z..Da.":-0.00065,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":6069210,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":16.32654,"Ion.Inject.Time..ms.":16.006,"RT..min.":8.6556,"First.Scan":6746,"Last.Scan":6746,"Master.Scan.s.":6736,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":1033,"XCorr":3.79,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":0.00024,"Percolator.SVMScore":1.211,"Precursor.Area":9484146,"Apex.RT..min.":8.67,"ptmRS..Binomial.Peptide.Score":267.95,"ptmRS..Isoform.Confidence.Probability":0.992,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(9): 990.58; y1+-H(3) O(4) P(8): 877.50; y1+-H(3) O(4) P(7): 740.44; y2+-H(3) O(4) P(12): 671.34; y2+-H(3) O(4) P(10): 544.32; y2+-H(3) O(4) P(9): 495.80; y2+-H(3) O(4) P(8): 439.25; y1+(4): 402.23","ptmRS..Best.Site.Probabilities":"S3(Phospho): 99.99; S9(Phospho): 99.18","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(3): 100.0; S(9): 99.2; T(11): 0.8"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"DFRHSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[K].DFRHSDsYsENETNHTNVPISSTGGTNNK.[T]","Modifications":"S7(Phospho); S9(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0176,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1123.45463,"Contaminant":"False","Aligned.m.z..Da.":1123.45463,"MH...Da.":3368.34935,"Theo..MH...Da.":3368.34888,"DeltaM..ppm.":0.14,"Deltam.z..Da.":0.00016,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3231871.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":8.6661,"First.Scan":6760,"Last.Scan":6760,"Master.Scan.s.":6749,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":940,"XCorr":7.4,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":7.458e-10,"Percolator.SVMScore":3.518,"Precursor.Area":4485992.5,"Apex.RT..min.":8.67,"ptmRS..Binomial.Peptide.Score":458.91,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(7): 827.34; y2+-H(3) O(4) P(24): 1274.56; y2+-H(3) O(4) P(23): 1217.05; b1+- -2H(3) O(4) P -2H(3) O(4) P(12): 1431.56; b1+- -2H(3) O(4) P -2H(3) O(4) P(14): 1646.65; b2+- -2H(3) O(4) P -2H(3) O(4) P(15): 892.36; b2+- -2H(3) O(4) P -2H(3) O(4) P(16): 942.88; b2+- -2H(3) O(4) P -2H(3) O(4) P(17): 999.90; b2+- -2H(3) O(4) P -2H(3) O(4) P(18): 1049.44; b2+- -2H(3) O(4) P -2H(3) O(4) P(20): 1154.50","ptmRS..Best.Site.Probabilities":"S5(Phospho): 100; S9(Phospho): 99.07","ptmRS..Phospho.Site.Probabilities":"S(5): 100.0; S(7): 0.9; Y(8): 0.0; S(9): 99.1; T(13): 0.0; T(16): 0.0; S(21): 0.0; S(22): 0.0; T(23): 0.0; T(26): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].mSEGITDIEEsQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S11(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1744,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1096.47224,"Contaminant":"False","Aligned.m.z..Da.":1096.47224,"MH...Da.":2191.93721,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":4.27,"Deltam.z..Da.":0.00468,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":970371,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":14.23289,"Ion.Inject.Time..ms.":22,"RT..min.":14.9737,"First.Scan":14954,"Last.Scan":14954,"Master.Scan.s.":14952,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":2135,"XCorr":4.3,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":4.244e-08,"Percolator.SVMScore":2.784,"ptmRS..Binomial.Peptide.Score":334.49,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(14): 1665.76; y1+-H(3) O(4) P(11): 1336.60; y1+-H(3) O(4) P(10): 1207.56; y1+-H(3) O(4) P(9): 1078.52; y1+(7): 881.44; y1+(6): 768.35; y1+(5): 640.29","ptmRS..Best.Site.Probabilities":"S11(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; T(6): 0.0; S(11): 100.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"GVFGYGFEEPSAIQQR","Annotated.Sequence":"[R].GVFGYGFEEPSAIQQR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":2,"DeltaScore":0.4615,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":595.62419,"Contaminant":"False","Aligned.m.z..Da.":595.62419,"MH...Da.":1784.85801,"Theo..MH...Da.":1784.86022,"DeltaM..ppm.":-1.24,"Deltam.z..Da.":-0.00074,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2557094.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":30.8505,"Ion.Inject.Time..ms.":22,"RT..min.":15.0691,"First.Scan":15079,"Last.Scan":15079,"Master.Scan.s.":15069,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":1373,"XCorr":2.08,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":0.00085,"Percolator.SVMScore":0.984,"Precursor.Area":3079211.5,"Apex.RT..min.":15.05,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"GVFGYGFEEPSAIQQR","Annotated.Sequence":"[R].GVFGYGFEEPSAIQQR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.6422,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":595.62541,"Contaminant":"False","Aligned.m.z..Da.":595.62541,"MH...Da.":1784.86169,"Theo..MH...Da.":1784.86022,"DeltaM..ppm.":0.82,"Deltam.z..Da.":0.00049,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1215669.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":27.22619,"Ion.Inject.Time..ms.":22,"RT..min.":15.0269,"First.Scan":15024,"Last.Scan":15024,"Master.Scan.s.":15017,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":1421,"XCorr":3.41,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":2.765e-06,"Percolator.SVMScore":2.026,"Precursor.Area":3079211.5,"Apex.RT..min.":15.05,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"GVFGYGFEEPSAIQQR","Annotated.Sequence":"[R].GVFGYGFEEPSAIQQR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6927,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":892.93382,"Contaminant":"False","Aligned.m.z..Da.":892.93382,"MH...Da.":1784.86036,"Theo..MH...Da.":1784.86022,"DeltaM..ppm.":0.08,"Deltam.z..Da.":0.00007,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3811547.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":15.0233,"First.Scan":15018,"Last.Scan":15018,"Master.Scan.s.":15017,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":1273,"XCorr":4.49,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":1.628e-13,"Percolator.SVMScore":5.049,"Precursor.Area":13308095,"Apex.RT..min.":15.05,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSSPLHRLSPTGK","Annotated.Sequence":"[R].MSsPLHRLsPTGK.[S]","Modifications":"S3(Phospho); S9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.4323,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":785.84592,"Contaminant":"False","Aligned.m.z..Da.":785.84592,"MH...Da.":1570.68456,"Theo..MH...Da.":1570.68486,"DeltaM..ppm.":-0.19,"Deltam.z..Da.":-0.00015,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":4293289,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":40.51448,"Ion.Inject.Time..ms.":22,"RT..min.":8.6753,"First.Scan":6772,"Last.Scan":6772,"Master.Scan.s.":6762,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":1061,"XCorr":3.03,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":0.00002,"Percolator.SVMScore":1.672,"Precursor.Area":3688668.5,"Apex.RT..min.":8.67,"ptmRS..Binomial.Peptide.Score":336.52,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(9): 1071.48; y2+-H(3) O(4) P(11): 627.82; y2+- -2H(3) O(4) P -2H(3) O(4) P(11): 578.83","ptmRS..Best.Site.Probabilities":"S3(Phospho): 100; S9(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(3): 100.0; S(9): 100.0; T(11): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"GSYVGIHSTGFK","Annotated.Sequence":"[K].GSYVGIHSTGFK.[D]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":2,"DeltaScore":0.5084,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":418.21594,"Contaminant":"False","Aligned.m.z..Da.":418.21594,"MH...Da.":1252.63327,"Theo..MH...Da.":1252.63206,"DeltaM..ppm.":0.96,"Deltam.z..Da.":0.0004,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":4033321,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":58.04429,"Ion.Inject.Time..ms.":12.904,"RT..min.":8.8227,"First.Scan":6965,"Last.Scan":6965,"Master.Scan.s.":6957,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":780,"XCorr":2.38,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":0.00003,"Percolator.SVMScore":1.612,"Precursor.Area":3160890,"Apex.RT..min.":8.82,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"GSYVGIHSTGFK","Annotated.Sequence":"[K].GSYVGIHSTGFK.[D]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.4974,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":418.21551,"Contaminant":"False","Aligned.m.z..Da.":418.21551,"MH...Da.":1252.63199,"Theo..MH...Da.":1252.63206,"DeltaM..ppm.":-0.06,"Deltam.z..Da.":-0.00002,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3374071.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":70.19592,"Ion.Inject.Time..ms.":15.126,"RT..min.":8.8381,"First.Scan":6984,"Last.Scan":6984,"Master.Scan.s.":6983,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":711,"XCorr":1.95,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":0.00003,"Percolator.SVMScore":1.596,"Precursor.Area":3160890,"Apex.RT..min.":8.82,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"VVYKFDDMELDENLLR","Annotated.Sequence":"[K].VVYKFDDmELDENLLR.[G]","Modifications":"M8(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.7121,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":672.33222,"Contaminant":"False","Aligned.m.z..Da.":672.33222,"MH...Da.":2014.98212,"Theo..MH...Da.":2014.97902,"DeltaM..ppm.":1.54,"Deltam.z..Da.":0.00103,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1232805.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":20.59797,"Ion.Inject.Time..ms.":22,"RT..min.":14.8951,"First.Scan":14853,"Last.Scan":14853,"Master.Scan.s.":14848,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":1646,"XCorr":3.3,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":1.243e-07,"Percolator.SVMScore":2.589,"Precursor.Area":1079653.5,"Apex.RT..min.":14.9,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"HSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[R].HSDsYsENETNHTNVPISSTGGTNNK.[T]","Modifications":"S4(Phospho); S6(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.1773,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":984.05575,"Contaminant":"False","Aligned.m.z..Da.":984.05575,"MH...Da.":2950.15271,"Theo..MH...Da.":2950.15241,"DeltaM..ppm.":0.1,"Deltam.z..Da.":0.0001,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":9356456,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":12.495,"RT..min.":8.0289,"First.Scan":5921,"Last.Scan":5921,"Master.Scan.s.":5917,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":1123,"XCorr":6.43,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":3.387e-09,"Percolator.SVMScore":3.243,"Precursor.Area":17868662,"Apex.RT..min.":8.04,"ptmRS..Binomial.Peptide.Score":660.95,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(6): 739.21; y2+-H(3) O(4) P(23): 1257.03; y2+-H(3) O(4) P(22): 1173.53; y2+-H(3) O(4) P(21): 1092.00; b1+- -2H(3) O(4) P -2H(3) O(4) P(6): 641.23; b1+- -2H(3) O(4) P -2H(3) O(4) P(11): 1228.45; b1+- -2H(3) O(4) P -2H(3) O(4) P(12): 1365.51; b1+- -2H(3) O(4) P -2H(3) O(4) P(15): 1679.67; b2+- -2H(3) O(4) P -2H(3) O(4) P(13): 733.78; b2+- -2H(3) O(4) P -2H(3) O(4) P(14): 790.80; b2+- -2H(3) O(4) P -2H(3) O(4) P(15): 840.34","ptmRS..Best.Site.Probabilities":"S4(Phospho): 100; S6(Phospho): 99.99","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(4): 100.0; Y(5): 0.0; S(6): 100.0; T(10): 0.0; T(13): 0.0; S(18): 0.0; S(19): 0.0; T(20): 0.0; T(23): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"HSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[R].HSDsYsENETNHTNVPISSTGGTNNK.[T]","Modifications":"S4(Phospho); S6(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":0,"Charge":4,"Original.Precursor.Charge":4,"DeltaScore":0.0955,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":738.2949,"Contaminant":"False","Aligned.m.z..Da.":738.2949,"MH...Da.":2950.15776,"Theo..MH...Da.":2950.15241,"DeltaM..ppm.":1.81,"Deltam.z..Da.":0.00134,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":4039447.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":12.76,"Ion.Inject.Time..ms.":17.748,"RT..min.":8.0399,"First.Scan":5936,"Last.Scan":5936,"Master.Scan.s.":5930,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":1131,"XCorr":6.28,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":3.434e-11,"Percolator.SVMScore":4.077,"Precursor.Area":4036386.25,"Apex.RT..min.":8.04,"ptmRS..Binomial.Peptide.Score":442.86,"ptmRS..Isoform.Confidence.Probability":0.991,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(5): 572.21","ptmRS..Best.Site.Probabilities":"S4(Phospho): 99.98; S6(Phospho): 99.14","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(4): 100.0; Y(5): 0.9; S(6): 99.1; T(10): 0.0; T(13): 0.0; S(18): 0.0; S(19): 0.0; T(20): 0.0; T(23): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KNQQLEEDLEESDTK","Annotated.Sequence":"[K].KNQQLEEDLEESDTK.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.5036,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":602.61844,"Contaminant":"False","Aligned.m.z..Da.":602.61844,"MH...Da.":1805.84077,"Theo..MH...Da.":1805.83994,"DeltaM..ppm.":0.46,"Deltam.z..Da.":0.00028,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3900163.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":79.57107,"Ion.Inject.Time..ms.":17.363,"RT..min.":8.4191,"First.Scan":6436,"Last.Scan":6436,"Master.Scan.s.":6424,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":1545,"XCorr":2.74,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":0.00003,"Percolator.SVMScore":1.617,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LEAESWQEK","Annotated.Sequence":"[K].LEAESWQEK.[Y]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.4137,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":560.26827,"Contaminant":"False","Aligned.m.z..Da.":560.26827,"MH...Da.":1119.52926,"Theo..MH...Da.":1119.53168,"DeltaM..ppm.":-2.17,"Deltam.z..Da.":-0.00121,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":4923704,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":25.78171,"Ion.Inject.Time..ms.":22,"RT..min.":8.397,"First.Scan":6406,"Last.Scan":6406,"Master.Scan.s.":6398,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":449,"XCorr":2.49,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":0.00078,"Percolator.SVMScore":1,"Precursor.Area":2601225,"Apex.RT..min.":8.39,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":5,"DeltaScore":0.0709,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.4061,"Contaminant":"False","Aligned.m.z..Da.":967.4061,"MH...Da.":2900.20375,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":1.52,"Deltam.z..Da.":0.00147,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":13772267,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":20.13744,"Ion.Inject.Time..ms.":8.466,"RT..min.":15.1558,"First.Scan":15189,"Last.Scan":15189,"Master.Scan.s.":15186,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":2599,"XCorr":6.77,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":2.475e-13,"Percolator.SVMScore":4.973,"Precursor.Area":113452016,"Apex.RT..min.":15.07,"ptmRS..Binomial.Peptide.Score":618.69,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(13): 1413.58; b1+-H(3) O(4) P(16): 1771.69; y1+-H(3) O(4) P(15): 1750.78; y1+-H(3) O(4) P(14): 1621.73; y1+-H(3) O(4) P(13): 1458.67; b2+-H(3) O(4) P(16): 886.35; b2+-H(3) O(4) P(18): 1014.90; y2+-H(3) O(4) P(14): 811.37; b1+(12): 1344.56; y1+(13): 1556.65","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"AAEAGETGAATSATEGDNNNNTAAGDKK","Annotated.Sequence":"[K].AAEAGETGAATsATEGDNNNNTAAGDKK.[G]","Modifications":"S12(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0229,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":906.04908,"Contaminant":"False","Aligned.m.z..Da.":906.04908,"MH...Da.":2716.13268,"Theo..MH...Da.":2716.13298,"DeltaM..ppm.":-0.11,"Deltam.z..Da.":-0.0001,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":13177810,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":15.481,"RT..min.":4.9932,"First.Scan":1929,"Last.Scan":1929,"Master.Scan.s.":1926,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":2553,"XCorr":7.85,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":8.37e-16,"Percolator.SVMScore":6.092,"Precursor.Area":9583348,"Apex.RT..min.":5,"ptmRS..Binomial.Peptide.Score":567.57,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(18): 1789.79; y1+-H(3) O(4) P(17): 1688.75; y2+-H(3) O(4) P(25): 1174.02; y2+-H(3) O(4) P(24): 1138.50; y2+-H(3) O(4) P(22): 1045.47; y2+-H(3) O(4) P(18): 895.40; y2+-H(3) O(4) P(17): 844.88","ptmRS..Best.Site.Probabilities":"S12(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"T(7): 0.0; T(11): 0.0; S(12): 100.0; T(14): 0.0; T(22): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"RKVEELTTK","Annotated.Sequence":"[R].RKVEELTTK.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":2,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.608,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":368.55221,"Contaminant":"False","Aligned.m.z..Da.":368.55221,"MH...Da.":1103.64209,"Theo..MH...Da.":1103.6419,"DeltaM..ppm.":0.17,"Deltam.z..Da.":0.00006,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":7361210.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":27.00064,"Ion.Inject.Time..ms.":10.192,"RT..min.":4.864,"First.Scan":1759,"Last.Scan":1759,"Master.Scan.s.":1757,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":190,"XCorr":3.75,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":1.05e-06,"Percolator.SVMScore":2.201,"Precursor.Area":10717119,"Apex.RT..min.":4.88,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DIFSNDELLSDAYDAK","Annotated.Sequence":"[K].DIFSNDELLSDAYDAK.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7637,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":908.41882,"Contaminant":"False","Aligned.m.z..Da.":908.41882,"MH...Da.":1815.83037,"Theo..MH...Da.":1815.82831,"DeltaM..ppm.":1.13,"Deltam.z..Da.":0.00103,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1063388.875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":17.0693,"First.Scan":17647,"Last.Scan":17647,"Master.Scan.s.":17643,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":1697,"XCorr":4.57,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":2.042e-09,"Percolator.SVMScore":3.335,"Precursor.Area":28513122,"Apex.RT..min.":17.1,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0137,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40358,"Contaminant":"False","Aligned.m.z..Da.":967.40358,"MH...Da.":2900.19619,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":-1.08,"Deltam.z..Da.":-0.00104,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":399012.78125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":21.24303,"Ion.Inject.Time..ms.":22,"RT..min.":17.0172,"First.Scan":17580,"Last.Scan":17580,"Master.Scan.s.":17578,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":2331,"XCorr":4.37,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":4.229e-07,"Percolator.SVMScore":2.367,"ptmRS..Binomial.Peptide.Score":342.98,"ptmRS..Isoform.Confidence.Probability":0.993,"ptmRS..Isoform.Group.Report":"b1+(12): 1344.56","ptmRS..Best.Site.Probabilities":"S13(Phospho): 99.26","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.7; S(13): 99.3; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KVEELTTK","Annotated.Sequence":"[R].KVEELTTK.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.3626,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":474.27432,"Contaminant":"False","Aligned.m.z..Da.":474.27432,"MH...Da.":947.54136,"Theo..MH...Da.":947.54079,"DeltaM..ppm.":0.61,"Deltam.z..Da.":0.00029,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3646229.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":35.13837,"Ion.Inject.Time..ms.":22,"RT..min.":4.928,"First.Scan":1845,"Last.Scan":1845,"Master.Scan.s.":1835,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":252,"XCorr":1.71,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":0.0006,"Percolator.SVMScore":1.047,"Precursor.Area":1767101.75,"Apex.RT..min.":4.92,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LQQTAFDKK","Annotated.Sequence":"[R].LQQTAFDKK.[S]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.4054,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":360.20112,"Contaminant":"False","Aligned.m.z..Da.":360.20112,"MH...Da.":1078.5888,"Theo..MH...Da.":1078.58914,"DeltaM..ppm.":-0.31,"Deltam.z..Da.":-0.00011,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":10637005,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":10.69121,"Ion.Inject.Time..ms.":10.329,"RT..min.":5.5174,"First.Scan":2618,"Last.Scan":2618,"Master.Scan.s.":2615,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":392,"XCorr":2.96,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":0.00026,"Percolator.SVMScore":1.201,"Precursor.Area":52945760,"Apex.RT..min.":5.55,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"AAEAGETGAATSATEGDNNNNTAAGDK","Annotated.Sequence":"[K].AAEAGETGAATSATEGDNNNNTAAGDK.[K]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.731,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":836.69513,"Contaminant":"False","Aligned.m.z..Da.":836.69513,"MH...Da.":2508.07085,"Theo..MH...Da.":2508.07169,"DeltaM..ppm.":-0.33,"Deltam.z..Da.":-0.00028,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3493404,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":14.702,"Ion.Inject.Time..ms.":22,"RT..min.":5.5192,"First.Scan":2621,"Last.Scan":2621,"Master.Scan.s.":2615,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":2747,"XCorr":7.1,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":1.677e-12,"Percolator.SVMScore":4.626,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"SQMSVELDDDADLDDELAQLK","Annotated.Sequence":"[R].SQmsVELDDDADLDDELAQLK.[G]","Modifications":"M3(Oxidation); S4(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q08421","Master.Protein.Descriptions":"Enhancer of translation termination 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ETT1 PE=1 SV=1","Protein.Accessions":"Q08421","Protein.Descriptions":"Enhancer of translation termination 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ETT1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.1194,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":816.00953,"Contaminant":"False","Aligned.m.z..Da.":816.00953,"MH...Da.":2446.01404,"Theo..MH...Da.":2446.02149,"DeltaM..ppm.":-3.04,"Deltam.z..Da.":-0.00248,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":445077,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":27.06822,"Ion.Inject.Time..ms.":22,"RT..min.":17.458,"First.Scan":18149,"Last.Scan":18149,"Master.Scan.s.":18137,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":1835,"XCorr":3.1,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":0.00067,"Percolator.SVMScore":1.027,"ptmRS..Binomial.Peptide.Score":303.56,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(4): 432.15; b1+-H(3) O(4) P(5): 531.22; b1+-H(3) O(4) P(6): 660.27; b1+-H(3) O(4) P(7): 773.35; b1+(2): 216.10; b1+(3): 363.13","ptmRS..Best.Site.Probabilities":"S4(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(1): 0.0; S(4): 100.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"IAEFPEEGIDPSTYLNN","Annotated.Sequence":"[K].IAEFPEEGIDPSTYLNN.[-]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7289,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":954.94871,"Contaminant":"False","Aligned.m.z..Da.":954.94871,"MH...Da.":1908.89014,"Theo..MH...Da.":1908.88616,"DeltaM..ppm.":2.08,"Deltam.z..Da.":0.00199,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":784549.0625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":9.15823,"Ion.Inject.Time..ms.":22,"RT..min.":17.4969,"First.Scan":18198,"Last.Scan":18198,"Master.Scan.s.":18189,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":1728,"XCorr":3.43,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":5.294e-07,"Percolator.SVMScore":2.326,"Precursor.Area":1063480.625,"Apex.RT..min.":17.52,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DIFSNDELLSDAYDAK","Annotated.Sequence":"[K].DIFSNDELLSDAYDAK.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":2,"DeltaScore":0.709,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":605.94808,"Contaminant":"False","Aligned.m.z..Da.":605.94808,"MH...Da.":1815.8297,"Theo..MH...Da.":1815.82831,"DeltaM..ppm.":0.76,"Deltam.z..Da.":0.00046,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":6915954,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":7.34244,"Ion.Inject.Time..ms.":15.937,"RT..min.":17.1195,"First.Scan":17712,"Last.Scan":17712,"Master.Scan.s.":17708,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":1570,"XCorr":3.23,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":3.624e-06,"Percolator.SVMScore":1.976,"Precursor.Area":7032743.5,"Apex.RT..min.":17.1,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KSDEPSRESTPVR","Annotated.Sequence":"[K].KSDEPsREsTPVR.[S]","Modifications":"S6(Phospho); S9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q08421","Master.Protein.Descriptions":"Enhancer of translation termination 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ETT1 PE=1 SV=1","Protein.Accessions":"Q08421","Protein.Descriptions":"Enhancer of translation termination 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ETT1 PE=1 SV=1","X..Missed.Cleavages":2,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1711,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":824.34147,"Contaminant":"False","Aligned.m.z..Da.":824.34147,"MH...Da.":1647.67567,"Theo..MH...Da.":1647.67752,"DeltaM..ppm.":-1.13,"Deltam.z..Da.":-0.00093,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":30577014,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":6.20618,"Ion.Inject.Time..ms.":6.007,"RT..min.":4.5595,"First.Scan":1359,"Last.Scan":1359,"Master.Scan.s.":1354,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":976,"XCorr":2.98,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":0.00003,"Percolator.SVMScore":1.581,"Precursor.Area":17675226,"Apex.RT..min.":4.56,"ptmRS..Binomial.Peptide.Score":345.19,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(8): 911.42; b1+-H(3) O(4) P(9): 998.45; b1+-H(3) O(4) P(10): 1179.47; b1+-H(3) O(4) P(12): 1375.59; y1+- -2H(3) O(4) P -2H(3) O(4) P(10): 1121.57; y1+- -2H(3) O(4) P -2H(3) O(4) P(9): 992.53; y2+- -2H(3) O(4) P -2H(3) O(4) P(10): 561.29; y2+- -2H(3) O(4) P -2H(3) O(4) P(9): 496.77; y1+(7): 924.43","ptmRS..Best.Site.Probabilities":"S6(Phospho): 99.5; T10(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(6): 99.5; S(9): 0.5; T(10): 100.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DIFSNDELLSDAYDAK","Annotated.Sequence":"[K].DIFSNDELLSDAYDAK.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.7347,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":605.94825,"Contaminant":"False","Aligned.m.z..Da.":605.94825,"MH...Da.":1815.83019,"Theo..MH...Da.":1815.82831,"DeltaM..ppm.":1.03,"Deltam.z..Da.":0.00063,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1547627.125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":14.62416,"Ion.Inject.Time..ms.":22,"RT..min.":17.0787,"First.Scan":17659,"Last.Scan":17659,"Master.Scan.s.":17656,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":1662,"XCorr":3.43,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":2.764e-06,"Percolator.SVMScore":2.026,"Precursor.Area":7032743.5,"Apex.RT..min.":17.1,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KSDEPSRESTPVR","Annotated.Sequence":"[K].KSDEPsRESTPVR.[S]","Modifications":"S6(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q08421","Master.Protein.Descriptions":"Enhancer of translation termination 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ETT1 PE=1 SV=1","Protein.Accessions":"Q08421","Protein.Descriptions":"Enhancer of translation termination 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ETT1 PE=1 SV=1","X..Missed.Cleavages":2,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0772,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":523.24269,"Contaminant":"False","Aligned.m.z..Da.":523.24269,"MH...Da.":1567.71351,"Theo..MH...Da.":1567.71119,"DeltaM..ppm.":1.48,"Deltam.z..Da.":0.00077,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":4582262.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":22.05223,"Ion.Inject.Time..ms.":16.811,"RT..min.":4.6901,"First.Scan":1532,"Last.Scan":1532,"Master.Scan.s.":1523,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":1120,"XCorr":2.98,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":0.00005,"Percolator.SVMScore":1.497,"Precursor.Area":6921721,"Apex.RT..min.":4.7,"ptmRS..Binomial.Peptide.Score":196.78,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y2+-H(3) O(4) P(12): 671.32; y2+-H(3) O(4) P(11): 627.81; y2+-H(3) O(4) P(10): 570.29; y2+-H(3) O(4) P(9): 505.77; y1+(5): 559.32; y2+(7): 422.74","ptmRS..Best.Site.Probabilities":"S6(Phospho): 99.99","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(6): 100.0; S(9): 0.0; T(10): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DLEQENVEK","Annotated.Sequence":"[K].DLEQENVEK.[E]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5436,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":552.26445,"Contaminant":"False","Aligned.m.z..Da.":552.26445,"MH...Da.":1103.52163,"Theo..MH...Da.":1103.52151,"DeltaM..ppm.":0.11,"Deltam.z..Da.":0.00006,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":5516480.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":8.09972,"Ion.Inject.Time..ms.":22,"RT..min.":6.4813,"First.Scan":3885,"Last.Scan":3885,"Master.Scan.s.":3876,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":571,"XCorr":2.41,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":0.001,"Percolator.SVMScore":0.955,"Precursor.Area":8681441,"Apex.RT..min.":6.49,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FDDMELDENLLR","Annotated.Sequence":"[K].FDDMELDENLLR.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5651,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":755.34723,"Contaminant":"False","Aligned.m.z..Da.":755.34723,"MH...Da.":1509.68719,"Theo..MH...Da.":1509.68898,"DeltaM..ppm.":-1.19,"Deltam.z..Da.":-0.0009,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1272914.625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":5.12561,"Ion.Inject.Time..ms.":22,"RT..min.":16.0859,"First.Scan":16386,"Last.Scan":16386,"Master.Scan.s.":16382,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":1009,"XCorr":3.61,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":3.641e-07,"Percolator.SVMScore":2.394,"Precursor.Area":7019004,"Apex.RT..min.":16.05,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KFQDDTLNR","Annotated.Sequence":"[K].KFQDDtLNR.[V]","Modifications":"T6(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6982,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":608.77157,"Contaminant":"False","Aligned.m.z..Da.":608.77157,"MH...Da.":1216.53586,"Theo..MH...Da.":1216.53579,"DeltaM..ppm.":0.05,"Deltam.z..Da.":0.00003,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":6060920,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":25.48891,"Ion.Inject.Time..ms.":22,"RT..min.":6.3988,"First.Scan":3775,"Last.Scan":3775,"Master.Scan.s.":3772,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":631,"XCorr":3.38,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":0.00007,"Percolator.SVMScore":1.433,"Precursor.Area":4554481.5,"Apex.RT..min.":6.4,"ptmRS..Binomial.Peptide.Score":330.62,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"T6(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"T(6): 100.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"QLSEDSHHLQSNNDNFSK","Annotated.Sequence":"[K].QLSEDSHHLQSNNDNFSK.[K]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":4,"Original.Precursor.Charge":4,"DeltaScore":0.5284,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":525.7398,"Contaminant":"False","Aligned.m.z..Da.":525.7398,"MH...Da.":2099.93738,"Theo..MH...Da.":2099.9377,"DeltaM..ppm.":-0.15,"Deltam.z..Da.":-0.00008,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":4191458.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":3.9749,"Ion.Inject.Time..ms.":15.367,"RT..min.":6.5965,"First.Scan":4036,"Last.Scan":4036,"Master.Scan.s.":4032,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":2217,"XCorr":3.35,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":0.00003,"Percolator.SVMScore":1.618,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LEAGLSDSK","Annotated.Sequence":"[K].LEAGLSDSK.[Q]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.3673,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":460.24017,"Contaminant":"False","Aligned.m.z..Da.":460.24017,"MH...Da.":919.47307,"Theo..MH...Da.":919.4731,"DeltaM..ppm.":-0.03,"Deltam.z..Da.":-1e-05,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":10937960,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":12.03204,"Ion.Inject.Time..ms.":11.203,"RT..min.":6.607,"First.Scan":4050,"Last.Scan":4050,"Master.Scan.s.":4045,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":418,"XCorr":2.75,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":0.00021,"Percolator.SVMScore":1.241,"Precursor.Area":11340871,"Apex.RT..min.":6.61,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.4011,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40879,"Contaminant":"False","Aligned.m.z..Da.":967.40879,"MH...Da.":2900.21181,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":4.3,"Deltam.z..Da.":0.00416,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":372843.71875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":20.34719,"Ion.Inject.Time..ms.":22,"RT..min.":16.5041,"First.Scan":16923,"Last.Scan":16923,"Master.Scan.s.":16915,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":2690,"XCorr":3.69,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":1.346e-06,"Percolator.SVMScore":2.156,"ptmRS..Binomial.Peptide.Score":358.98,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b2+-H(3) O(4) P(18): 1014.90; y2+-H(3) O(4) P(14): 811.37","ptmRS..Best.Site.Probabilities":"S13(Phospho): 99.99","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MFILDEADEMLSSGFK","Annotated.Sequence":"[K].mFILDEADEmLSSGFK.[E]","Modifications":"M1(Oxidation); M10(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.5957,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":622.28144,"Contaminant":"False","Aligned.m.z..Da.":622.28144,"MH...Da.":1864.82978,"Theo..MH...Da.":1864.83433,"DeltaM..ppm.":-2.44,"Deltam.z..Da.":-0.00152,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1224665.625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":4.9155,"Ion.Inject.Time..ms.":22,"RT..min.":16.6861,"First.Scan":17155,"Last.Scan":17155,"Master.Scan.s.":17149,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":1829,"XCorr":3.24,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":9.719e-07,"Percolator.SVMScore":2.215,"Precursor.Area":3832247,"Apex.RT..min.":16.72,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MFILDEADEMLSSGFK","Annotated.Sequence":"[K].mFILDEADEmLSSGFK.[E]","Modifications":"M1(Oxidation); M10(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7506,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":932.91746,"Contaminant":"False","Aligned.m.z..Da.":932.91746,"MH...Da.":1864.82765,"Theo..MH...Da.":1864.83433,"DeltaM..ppm.":-3.58,"Deltam.z..Da.":-0.00334,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1922856.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":16.6849,"First.Scan":17153,"Last.Scan":17153,"Master.Scan.s.":17149,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":1674,"XCorr":3.85,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":1.73e-08,"Percolator.SVMScore":2.947,"Precursor.Area":5874701,"Apex.RT..min.":16.72,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LQQTAFDKK","Annotated.Sequence":"[R].LQQTAFDKK.[S]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":2,"DeltaScore":0.2723,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":360.20099,"Contaminant":"False","Aligned.m.z..Da.":360.20099,"MH...Da.":1078.58843,"Theo..MH...Da.":1078.58914,"DeltaM..ppm.":-0.65,"Deltam.z..Da.":-0.00023,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":65937780,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":8.89812,"Ion.Inject.Time..ms.":3.389,"RT..min.":5.5494,"First.Scan":2661,"Last.Scan":2661,"Master.Scan.s.":2654,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":383,"XCorr":2.02,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":0.0004,"Percolator.SVMScore":1.123,"Precursor.Area":52945760,"Apex.RT..min.":5.55,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"AAEAGETGAATSATEGDNNNNTAAGDK","Annotated.Sequence":"[K].AAEAGETGAATsATEGDNNNNTAAGDK.[K]","Modifications":"S12(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0104,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":863.35192,"Contaminant":"False","Aligned.m.z..Da.":863.35192,"MH...Da.":2588.04122,"Theo..MH...Da.":2588.03802,"DeltaM..ppm.":1.24,"Deltam.z..Da.":0.00107,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3378637.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":5.6661,"First.Scan":2814,"Last.Scan":2814,"Master.Scan.s.":2810,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":1621,"XCorr":5.76,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":2.303e-10,"Percolator.SVMScore":3.732,"Precursor.Area":4306885,"Apex.RT..min.":5.67,"ptmRS..Binomial.Peptide.Score":456.51,"ptmRS..Isoform.Confidence.Probability":0.987,"ptmRS..Isoform.Group.Report":"b2+-H(3) O(4) P(26): 1172.48; y2+-H(3) O(4) P(21): 981.43; y2+-H(3) O(4) P(16): 780.83; b1+(7): 630.27; b1+(9): 758.33; b1+(10): 829.37","ptmRS..Best.Site.Probabilities":"S12(Phospho): 98.7","ptmRS..Phospho.Site.Probabilities":"T(7): 0.7; T(11): 0.7; S(12): 98.7; T(14): 0.0; T(22): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"APQALMLAPTR","Annotated.Sequence":"[K].APQALMLAPTR.[E]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6742,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":584.829,"Contaminant":"False","Aligned.m.z..Da.":584.829,"MH...Da.":1168.65073,"Theo..MH...Da.":1168.65069,"DeltaM..ppm.":0.03,"Deltam.z..Da.":0.00002,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3848638.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":61.90895,"Ion.Inject.Time..ms.":20.476,"RT..min.":11.6049,"First.Scan":10602,"Last.Scan":10602,"Master.Scan.s.":10597,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":162,"XCorr":3.99,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":2.417e-08,"Percolator.SVMScore":2.886,"Precursor.Area":9562037,"Apex.RT..min.":11.63,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MGSINNNFNTNNNSNTDLDR","Annotated.Sequence":"[-].mGSINNNFNTNNNsNTDLDR.[D]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S14(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P39517","Master.Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","Protein.Accessions":"P39517","Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0689,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":749.31097,"Contaminant":"False","Aligned.m.z..Da.":749.31097,"MH...Da.":2245.91836,"Theo..MH...Da.":2245.91057,"DeltaM..ppm.":3.47,"Deltam.z..Da.":0.0026,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1746904.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":8.81062,"Ion.Inject.Time..ms.":22,"RT..min.":11.9801,"First.Scan":11090,"Last.Scan":11090,"Master.Scan.s.":11078,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":1478,"XCorr":3.63,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":0.00002,"Percolator.SVMScore":1.668,"ptmRS..Binomial.Peptide.Score":287,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(12): 1359.59; y1+-H(3) O(4) P(11): 1245.55; y1+-H(3) O(4) P(10): 1144.50; y1+-H(3) O(4) P(9): 1030.45; y1+-H(3) O(4) P(8): 916.41; y1+-H(3) O(4) P(7): 802.37; y2+-H(3) O(4) P(7): 401.69; y1+(6): 733.35; y1+(5): 619.30","ptmRS..Best.Site.Probabilities":"S14(Phospho): 99.99","ptmRS..Phospho.Site.Probabilities":"S(3): 0.0; T(10): 0.0; S(14): 100.0; T(16): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"TAVFYGGTPISK","Annotated.Sequence":"[K].TAVFYGGTPISK.[D]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5704,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":620.83236,"Contaminant":"False","Aligned.m.z..Da.":620.83236,"MH...Da.":1240.65744,"Theo..MH...Da.":1240.65721,"DeltaM..ppm.":0.19,"Deltam.z..Da.":0.00011,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3194083.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":25.42651,"Ion.Inject.Time..ms.":22,"RT..min.":11.1267,"First.Scan":9979,"Last.Scan":9979,"Master.Scan.s.":9973,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":385,"XCorr":2.91,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":0.00003,"Percolator.SVMScore":1.565,"Precursor.Area":5615649,"Apex.RT..min.":11.14,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"TVEPDAVDLLEK","Annotated.Sequence":"[K].TVEPDAVDLLEK.[M]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P32485","Master.Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","Protein.Accessions":"P32485","Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6133,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":664.85143,"Contaminant":"False","Aligned.m.z..Da.":664.85143,"MH...Da.":1328.69559,"Theo..MH...Da.":1328.69439,"DeltaM..ppm.":0.91,"Deltam.z..Da.":0.0006,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":960163.1875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":26.44733,"Ion.Inject.Time..ms.":22,"RT..min.":13.7323,"First.Scan":13355,"Last.Scan":13355,"Master.Scan.s.":13353,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":513,"XCorr":2.56,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":5.968e-06,"Percolator.SVMScore":1.886,"Precursor.Area":852539.1875,"Apex.RT..min.":13.73,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"SSQIHIENESTEDILK","Annotated.Sequence":"[R].SSQIHIENEsTEDILK.[I]","Modifications":"S10(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0088,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":641.62983,"Contaminant":"False","Aligned.m.z..Da.":641.62983,"MH...Da.":1922.87495,"Theo..MH...Da.":1922.87429,"DeltaM..ppm.":0.34,"Deltam.z..Da.":0.00022,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1471021.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":22.09808,"Ion.Inject.Time..ms.":22,"RT..min.":11.3324,"First.Scan":10247,"Last.Scan":10247,"Master.Scan.s.":10246,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":1714,"XCorr":3.41,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":0.00002,"Percolator.SVMScore":1.676,"Precursor.Area":1538187,"Apex.RT..min.":11.32,"ptmRS..Binomial.Peptide.Score":257.11,"ptmRS..Isoform.Confidence.Probability":0.007,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(10): 1159.55; y1+-H(3) O(4) P(7): 787.42; y1+-H(3) O(4) P(6): 700.39; y2+-H(3) O(4) P(7): 394.21","ptmRS..Best.Site.Probabilities":"T11(Phospho): 99.3","ptmRS..Phospho.Site.Probabilities":"S(1): 0.0; S(2): 0.0; S(10): 0.7; T(11): 99.3"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"MGSINNNFNTNNNSNTDLDRDWK","Annotated.Sequence":"[-].mGSINNNFNTNNNsNTDLDRDWK.[T]","Modifications":"N-Term(Prot)(Met-loss); S14(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P39517","Master.Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","Protein.Accessions":"P39517","Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.033,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":878.3718,"Contaminant":"False","Aligned.m.z..Da.":878.3718,"MH...Da.":2633.10085,"Theo..MH...Da.":2633.10123,"DeltaM..ppm.":-0.14,"Deltam.z..Da.":-0.00012,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":12610758,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":4.23853,"Ion.Inject.Time..ms.":16.503,"RT..min.":11.3138,"First.Scan":10223,"Last.Scan":10223,"Master.Scan.s.":10220,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":2193,"XCorr":6.37,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":4.366e-14,"Percolator.SVMScore":5.288,"Precursor.Area":8373583,"Apex.RT..min.":11.31,"ptmRS..Binomial.Peptide.Score":711.74,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(13): 1573.70; y1+-H(3) O(4) P(12): 1459.66; y1+-H(3) O(4) P(11): 1345.61; y1+-H(3) O(4) P(10): 1231.57; y2+-H(3) O(4) P(19): 1139.50; y2+-H(3) O(4) P(18): 1082.48; y2+-H(3) O(4) P(17): 1025.45; y2+-H(3) O(4) P(16): 968.43; y2+-H(3) O(4) P(15): 894.90; y2+-H(3) O(4) P(14): 837.88; y2+-H(3) O(4) P(13): 787.35; y2+-H(3) O(4) P(12): 730.33; y2+-H(3) O(4) P(11): 673.31; y2+-H(3) O(4) P(10): 616.29; y1+(9): 1162.55; y1+(8): 1048.51","ptmRS..Best.Site.Probabilities":"S14(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(3): 0.0; T(10): 0.0; S(14): 100.0; T(16): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0701,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":953.40263,"Contaminant":"False","Aligned.m.z..Da.":953.40263,"MH...Da.":2858.19334,"Theo..MH...Da.":2858.18876,"DeltaM..ppm.":1.6,"Deltam.z..Da.":0.00153,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":504593.6875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":13.7976,"First.Scan":13441,"Last.Scan":13441,"Master.Scan.s.":13431,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":2505,"XCorr":5.71,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":9.751e-12,"Percolator.SVMScore":4.306,"Precursor.Area":3359208.25,"Apex.RT..min.":13.83,"ptmRS..Binomial.Peptide.Score":398.09,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(13): 1458.67; y2+-H(3) O(4) P(14): 811.37; b1+(12): 1302.55","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"TGTFSIAALQR","Annotated.Sequence":"[K].TGTFSIAALQR.[I]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7742,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":582.82365,"Contaminant":"False","Aligned.m.z..Da.":582.82365,"MH...Da.":1164.64002,"Theo..MH...Da.":1164.63715,"DeltaM..ppm.":2.47,"Deltam.z..Da.":0.00144,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2195300.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":59.02114,"Ion.Inject.Time..ms.":22,"RT..min.":13.3523,"First.Scan":12865,"Last.Scan":12865,"Master.Scan.s.":12859,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":379,"XCorr":3.41,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":1.17e-06,"Percolator.SVMScore":2.182,"Precursor.Area":20433666,"Apex.RT..min.":13.38,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"GLAISFVSSK","Annotated.Sequence":"[K].GLAISFVSSK.[E]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6202,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":504.79012,"Contaminant":"False","Aligned.m.z..Da.":504.79012,"MH...Da.":1008.57296,"Theo..MH...Da.":1008.57242,"DeltaM..ppm.":0.53,"Deltam.z..Da.":0.00027,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1548219.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":43.40279,"Ion.Inject.Time..ms.":22,"RT..min.":13.3315,"First.Scan":12838,"Last.Scan":12838,"Master.Scan.s.":12833,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":208,"XCorr":2.58,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":0.00075,"Percolator.SVMScore":1.008,"Precursor.Area":1127440,"Apex.RT..min.":13.34,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"AIMPIIEGHDVLAQAQSGTGK","Annotated.Sequence":"[R].AImPIIEGHDVLAQAQSGTGK.[T]","Modifications":"M3(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.6926,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":718.03961,"Contaminant":"False","Aligned.m.z..Da.":718.03961,"MH...Da.":2152.10428,"Theo..MH...Da.":2152.10668,"DeltaM..ppm.":-1.11,"Deltam.z..Da.":-0.0008,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2313141.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":28.46422,"Ion.Inject.Time..ms.":22,"RT..min.":12.7105,"First.Scan":12035,"Last.Scan":12035,"Master.Scan.s.":12027,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":988,"XCorr":4.88,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":1.146e-14,"Percolator.SVMScore":5.531,"Precursor.Area":13837151,"Apex.RT..min.":12.76,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"VHACIGGTSFVEDAEGLR","Annotated.Sequence":"[K].VHAcIGGTSFVEDAEGLR.[D]","Modifications":"C4(Carbamidomethyl)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.72,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":639.97555,"Contaminant":"False","Aligned.m.z..Da.":639.97555,"MH...Da.":1917.91211,"Theo..MH...Da.":1917.91234,"DeltaM..ppm.":-0.12,"Deltam.z..Da.":-0.00008,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1967177.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":48.089,"Ion.Inject.Time..ms.":22,"RT..min.":13.1534,"First.Scan":12609,"Last.Scan":12609,"Master.Scan.s.":12599,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":1558,"XCorr":4.5,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":5.906e-09,"Percolator.SVMScore":3.142,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DFLLKPELSR","Annotated.Sequence":"[K].DFLLKPELSR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.6036,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":406.56747,"Contaminant":"False","Aligned.m.z..Da.":406.56747,"MH...Da.":1217.68786,"Theo..MH...Da.":1217.68885,"DeltaM..ppm.":-0.81,"Deltam.z..Da.":-0.00033,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2143558,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":7.25241,"Ion.Inject.Time..ms.":22,"RT..min.":13.1515,"First.Scan":12606,"Last.Scan":12606,"Master.Scan.s.":12599,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":285,"XCorr":2.75,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":0.00005,"Percolator.SVMScore":1.508,"Precursor.Area":5850717,"Apex.RT..min.":13.18,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"QFYVNVEEEEYK","Annotated.Sequence":"[K].QFYVNVEEEEYK.[Y]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6057,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":788.8619,"Contaminant":"False","Aligned.m.z..Da.":788.8619,"MH...Da.":1576.71653,"Theo..MH...Da.":1576.71658,"DeltaM..ppm.":-0.03,"Deltam.z..Da.":-0.00002,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2419545.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":58.59328,"Ion.Inject.Time..ms.":22,"RT..min.":12.2598,"First.Scan":11453,"Last.Scan":11453,"Master.Scan.s.":11442,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":1096,"XCorr":3.17,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":1.899e-07,"Percolator.SVMScore":2.512,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"GVAINFVTNEDVGAMRELEK","Annotated.Sequence":"[K].GVAINFVTNEDVGAmRELEK.[F]","Modifications":"M15(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.6447,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":736.70364,"Contaminant":"False","Aligned.m.z..Da.":736.70364,"MH...Da.":2208.09636,"Theo..MH...Da.":2208.09651,"DeltaM..ppm.":-0.07,"Deltam.z..Da.":-0.00005,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1679326,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":14.58059,"Ion.Inject.Time..ms.":22,"RT..min.":13.3946,"First.Scan":12921,"Last.Scan":12921,"Master.Scan.s.":12911,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":1451,"XCorr":4.56,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":2.281e-10,"Percolator.SVMScore":3.733,"Precursor.Area":2001306.625,"Apex.RT..min.":13.41,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"GVAINFVTNEDVGAMR","Annotated.Sequence":"[K].GVAINFVTNEDVGAmR.[E]","Modifications":"M15(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6818,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":854.91749,"Contaminant":"False","Aligned.m.z..Da.":854.91749,"MH...Da.":1708.82771,"Theo..MH...Da.":1708.8323,"DeltaM..ppm.":-2.69,"Deltam.z..Da.":-0.00229,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":702890.1875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":43.63281,"Ion.Inject.Time..ms.":22,"RT..min.":13.4606,"First.Scan":13004,"Last.Scan":13004,"Master.Scan.s.":13002,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":1272,"XCorr":2.64,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":0.00001,"Percolator.SVMScore":1.738,"Precursor.Area":653683.8125,"Apex.RT..min.":13.45,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"NQQLEEDLEESDTK","Annotated.Sequence":"[K].NQQLEEDLEESDTK.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6971,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":839.37553,"Contaminant":"False","Aligned.m.z..Da.":839.37553,"MH...Da.":1677.74378,"Theo..MH...Da.":1677.74498,"DeltaM..ppm.":-0.71,"Deltam.z..Da.":-0.0006,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":4270572.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":25.42527,"Ion.Inject.Time..ms.":22,"RT..min.":9.6572,"First.Scan":8061,"Last.Scan":8061,"Master.Scan.s.":8049,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":1457,"XCorr":4.16,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":1.745e-09,"Percolator.SVMScore":3.364,"Precursor.Area":2539544.75,"Apex.RT..min.":9.65,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].mSEGITDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6781,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1056.4852,"Contaminant":"False","Aligned.m.z..Da.":1056.4852,"MH...Da.":2111.96312,"Theo..MH...Da.":2111.96151,"DeltaM..ppm.":0.76,"Deltam.z..Da.":0.0008,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2783992.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":14.6102,"First.Scan":14487,"Last.Scan":14487,"Master.Scan.s.":14484,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":2372,"XCorr":5.56,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":5.048e-13,"Percolator.SVMScore":4.844,"Precursor.Area":6600502.5,"Apex.RT..min.":14.63,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"AIMPIIEGHDVLAQAQSGTGK","Annotated.Sequence":"[R].AIMPIIEGHDVLAQAQSGTGK.[T]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":2,"DeltaScore":0.1168,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":712.70844,"Contaminant":"False","Aligned.m.z..Da.":712.70844,"MH...Da.":2136.11076,"Theo..MH...Da.":2136.11176,"DeltaM..ppm.":-0.47,"Deltam.z..Da.":-0.00033,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2134874.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":82.95361,"Ion.Inject.Time..ms.":22,"RT..min.":14.5699,"First.Scan":14435,"Last.Scan":14435,"Master.Scan.s.":14432,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":964,"XCorr":1.37,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":0.00076,"Percolator.SVMScore":1.005,"Precursor.Area":1509684,"Apex.RT..min.":14.56,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"AIMPIIEGHDVLAQAQSGTGK","Annotated.Sequence":"[R].AIMPIIEGHDVLAQAQSGTGK.[T]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.6357,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":712.70917,"Contaminant":"False","Aligned.m.z..Da.":712.70917,"MH...Da.":2136.11297,"Theo..MH...Da.":2136.11176,"DeltaM..ppm.":0.56,"Deltam.z..Da.":0.0004,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2151770,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":38.78829,"Ion.Inject.Time..ms.":22,"RT..min.":14.5599,"First.Scan":14422,"Last.Scan":14422,"Master.Scan.s.":14419,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":943,"XCorr":3.87,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":3.552e-11,"Percolator.SVMScore":4.071,"Precursor.Area":1509684,"Apex.RT..min.":14.56,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"IQDPQMTGYVSTR","Annotated.Sequence":"[R].IQDPQMtGYVsTR.[Y]","Modifications":"T7(Phospho); S11(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P32485","Master.Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","Protein.Accessions":"P32485","Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.0321,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":828.33123,"Contaminant":"False","Aligned.m.z..Da.":828.33123,"MH...Da.":1655.65519,"Theo..MH...Da.":1655.65362,"DeltaM..ppm.":0.95,"Deltam.z..Da.":0.00079,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":4456666.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":16.5106,"Ion.Inject.Time..ms.":22,"RT..min.":9.8531,"First.Scan":8317,"Last.Scan":8317,"Master.Scan.s.":8309,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":690,"XCorr":2.8,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":0.0002,"Percolator.SVMScore":1.25,"Precursor.Area":5790401,"Apex.RT..min.":9.87,"ptmRS..Binomial.Peptide.Score":365.49,"ptmRS..Isoform.Confidence.Probability":0.004,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(11): 1316.53; y1+-H(3) O(4) P(10): 1201.51; y1+-H(3) O(4) P(8): 976.40; y1+-H(3) O(4) P(7): 845.36; y2+-H(3) O(4) P(11): 658.77; y2+-H(3) O(4) P(10): 601.26; y1+(4): 462.27; y1+(3): 363.20","ptmRS..Best.Site.Probabilities":"T7(Phospho): 100; Y9(Phospho): 99.58","ptmRS..Phospho.Site.Probabilities":"T(7): 100.0; Y(9): 99.6; S(11): 0.4; T(12): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DFKPQALGDTNLFKPIK","Annotated.Sequence":"[K].DFKPQALGDTNLFKPIK.[I]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q03558","Master.Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","Protein.Accessions":"Q03558","Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.5938,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":644.69151,"Contaminant":"False","Aligned.m.z..Da.":644.69151,"MH...Da.":1932.05998,"Theo..MH...Da.":1932.05892,"DeltaM..ppm.":0.55,"Deltam.z..Da.":0.00035,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1334752.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":31.81493,"Ion.Inject.Time..ms.":22,"RT..min.":14.3796,"First.Scan":14189,"Last.Scan":14189,"Master.Scan.s.":14185,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":341,"XCorr":2.88,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":2.55e-08,"Percolator.SVMScore":2.877,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FDDMELDENLLR","Annotated.Sequence":"[K].FDDmELDENLLR.[G]","Modifications":"M4(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5021,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":763.34542,"Contaminant":"False","Aligned.m.z..Da.":763.34542,"MH...Da.":1525.68357,"Theo..MH...Da.":1525.6839,"DeltaM..ppm.":-0.22,"Deltam.z..Da.":-0.00017,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1850077.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":87.7169,"Ion.Inject.Time..ms.":22,"RT..min.":14.4504,"First.Scan":14281,"Last.Scan":14281,"Master.Scan.s.":14276,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":1082,"XCorr":2.35,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":1.164e-06,"Percolator.SVMScore":2.183,"Precursor.Area":15268920,"Apex.RT..min.":14.48,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0608,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40237,"Contaminant":"False","Aligned.m.z..Da.":967.40237,"MH...Da.":2900.19256,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":-2.33,"Deltam.z..Da.":-0.00226,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1001308.875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":14.7921,"First.Scan":14721,"Last.Scan":14721,"Master.Scan.s.":14718,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":2163,"XCorr":5.59,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":1.097e-09,"Percolator.SVMScore":3.448,"Precursor.Area":2342689.5,"Apex.RT..min.":14.81,"ptmRS..Binomial.Peptide.Score":495.98,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(13): 1413.58; y1+-H(3) O(4) P(14): 1621.73; y1+-H(3) O(4) P(13): 1458.67; y2+-H(3) O(4) P(14): 811.37; b1+(12): 1344.56; y1+(13): 1556.65","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DAQIVVGTPGR","Annotated.Sequence":"[R].DAQIVVGTPGR.[V]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6702,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":556.8061,"Contaminant":"False","Aligned.m.z..Da.":556.8061,"MH...Da.":1112.60493,"Theo..MH...Da.":1112.60585,"DeltaM..ppm.":-0.83,"Deltam.z..Da.":-0.00046,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":6350539.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":3.40798,"Ion.Inject.Time..ms.":22,"RT..min.":9.1598,"First.Scan":7407,"Last.Scan":7407,"Master.Scan.s.":7399,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":249,"XCorr":2.85,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":0.00031,"Percolator.SVMScore":1.165,"Precursor.Area":6254742.5,"Apex.RT..min.":9.17,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LQETNPEEVPKFEK","Annotated.Sequence":"[K].LQETNPEEVPKFEK.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.713,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":844.42866,"Contaminant":"False","Aligned.m.z..Da.":844.42866,"MH...Da.":1687.85003,"Theo..MH...Da.":1687.85374,"DeltaM..ppm.":-2.2,"Deltam.z..Da.":-0.00185,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2704886,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":3.79938,"Ion.Inject.Time..ms.":22,"RT..min.":9.3637,"First.Scan":7674,"Last.Scan":7674,"Master.Scan.s.":7672,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":973,"XCorr":4.32,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":5.417e-07,"Percolator.SVMScore":2.322,"Precursor.Area":2013818.375,"Apex.RT..min.":9.35,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FFISNPDLVDR","Annotated.Sequence":"[R].FFISNPDLVDR.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q03558","Master.Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","Protein.Accessions":"Q03558","Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7814,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":661.84014,"Contaminant":"False","Aligned.m.z..Da.":661.84014,"MH...Da.":1322.673,"Theo..MH...Da.":1322.67393,"DeltaM..ppm.":-0.7,"Deltam.z..Da.":-0.00046,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3146013,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":18.98015,"Ion.Inject.Time..ms.":22,"RT..min.":14.6507,"First.Scan":14539,"Last.Scan":14539,"Master.Scan.s.":14536,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":640,"XCorr":3.34,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":5.102e-06,"Percolator.SVMScore":1.914,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FFISNPDLVDR","Annotated.Sequence":"[R].FFISNPDLVDR.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q03558","Master.Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","Protein.Accessions":"Q03558","Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7292,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":661.83934,"Contaminant":"False","Aligned.m.z..Da.":661.83934,"MH...Da.":1322.67141,"Theo..MH...Da.":1322.67393,"DeltaM..ppm.":-1.9,"Deltam.z..Da.":-0.00126,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":9403101,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":15.50677,"Ion.Inject.Time..ms.":12.76,"RT..min.":14.6695,"First.Scan":14563,"Last.Scan":14563,"Master.Scan.s.":14562,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":662,"XCorr":3.36,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":3.93e-06,"Percolator.SVMScore":1.962,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LQETNPEEVPKFEK","Annotated.Sequence":"[K].LQETNPEEVPKFEK.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.7461,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":563.28878,"Contaminant":"False","Aligned.m.z..Da.":563.28878,"MH...Da.":1687.85179,"Theo..MH...Da.":1687.85374,"DeltaM..ppm.":-1.16,"Deltam.z..Da.":-0.00065,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":19228614,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":15.98421,"Ion.Inject.Time..ms.":4.849,"RT..min.":9.3438,"First.Scan":7648,"Last.Scan":7648,"Master.Scan.s.":7646,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":880,"XCorr":3.86,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":1.1e-06,"Percolator.SVMScore":2.193,"Precursor.Area":19702512,"Apex.RT..min.":9.35,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].mSEGITDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.6453,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":704.65804,"Contaminant":"False","Aligned.m.z..Da.":704.65804,"MH...Da.":2111.95957,"Theo..MH...Da.":2111.96151,"DeltaM..ppm.":-0.92,"Deltam.z..Da.":-0.00065,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1176434.875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":7.30829,"Ion.Inject.Time..ms.":22,"RT..min.":14.6214,"First.Scan":14502,"Last.Scan":14502,"Master.Scan.s.":14497,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":2274,"XCorr":4.37,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":1.372e-07,"Percolator.SVMScore":2.571,"Precursor.Area":1788950.25,"Apex.RT..min.":14.63,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FFISNPDLVDR","Annotated.Sequence":"[R].FFISNPDLVDR.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q03558","Master.Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","Protein.Accessions":"Q03558","Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":3,"DeltaScore":0.3208,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":661.84026,"Contaminant":"False","Aligned.m.z..Da.":661.84026,"MH...Da.":1322.67324,"Theo..MH...Da.":1322.67393,"DeltaM..ppm.":-0.52,"Deltam.z..Da.":-0.00034,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":4309836.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":70.55193,"Ion.Inject.Time..ms.":22,"RT..min.":14.6905,"First.Scan":14590,"Last.Scan":14590,"Master.Scan.s.":14588,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":642,"XCorr":2.12,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":0.00075,"Percolator.SVMScore":1.006,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"APQALMLAPTR","Annotated.Sequence":"[K].APQALmLAPTR.[E]","Modifications":"M6(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7642,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":592.82613,"Contaminant":"False","Aligned.m.z..Da.":592.82613,"MH...Da.":1184.64498,"Theo..MH...Da.":1184.6456,"DeltaM..ppm.":-0.53,"Deltam.z..Da.":-0.00031,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3781633.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":43.83511,"Ion.Inject.Time..ms.":22,"RT..min.":10.1526,"First.Scan":8711,"Last.Scan":8711,"Master.Scan.s.":8699,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":327,"XCorr":3.35,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":3.643e-07,"Percolator.SVMScore":2.394,"Precursor.Area":28479162,"Apex.RT..min.":10.19,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DFKPQALGDTNLFKPIK","Annotated.Sequence":"[K].DFKPQALGDTNLFKPIK.[I]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q03558","Master.Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","Protein.Accessions":"Q03558","Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":4,"Original.Precursor.Charge":4,"DeltaScore":0.7213,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":483.77042,"Contaminant":"False","Aligned.m.z..Da.":483.77042,"MH...Da.":1932.05985,"Theo..MH...Da.":1932.05892,"DeltaM..ppm.":0.48,"Deltam.z..Da.":0.00023,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1134172.625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":38.50723,"Ion.Inject.Time..ms.":22,"RT..min.":14.3733,"First.Scan":14182,"Last.Scan":14182,"Master.Scan.s.":14172,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":342,"XCorr":3.05,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":1.267e-06,"Percolator.SVMScore":2.167,"Precursor.Area":1690786,"Apex.RT..min.":14.39,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"AGNFALHPEVVR","Annotated.Sequence":"[R].AGNFALHPEVVR.[E]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q03558","Master.Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","Protein.Accessions":"Q03558","Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.5948,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":437.23844,"Contaminant":"False","Aligned.m.z..Da.":437.23844,"MH...Da.":1309.70077,"Theo..MH...Da.":1309.70114,"DeltaM..ppm.":-0.29,"Deltam.z..Da.":-0.00013,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":6432578,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":10.67535,"Ion.Inject.Time..ms.":20.442,"RT..min.":10.0687,"First.Scan":8599,"Last.Scan":8599,"Master.Scan.s.":8595,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":393,"XCorr":3.43,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":6.188e-08,"Percolator.SVMScore":2.716,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KDELTLEGIK","Annotated.Sequence":"[K].KDELTLEGIK.[Q]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.5853,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":382.55177,"Contaminant":"False","Aligned.m.z..Da.":382.55177,"MH...Da.":1145.64077,"Theo..MH...Da.":1145.64123,"DeltaM..ppm.":-0.4,"Deltam.z..Da.":-0.00015,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":10798129,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":11.689,"RT..min.":10.2782,"First.Scan":8873,"Last.Scan":8873,"Master.Scan.s.":8868,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":314,"XCorr":3.4,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":0.00036,"Percolator.SVMScore":1.139,"Precursor.Area":18505104,"Apex.RT..min.":10.29,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KDELTLEGIK","Annotated.Sequence":"[K].KDELTLEGIK.[Q]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5262,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":573.324,"Contaminant":"False","Aligned.m.z..Da.":573.324,"MH...Da.":1145.64072,"Theo..MH...Da.":1145.64123,"DeltaM..ppm.":-0.44,"Deltam.z..Da.":-0.00025,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":16443439,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":1.93113,"Ion.Inject.Time..ms.":9.158,"RT..min.":10.2771,"First.Scan":8871,"Last.Scan":8871,"Master.Scan.s.":8868,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":314,"XCorr":4.01,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":1.964e-07,"Percolator.SVMScore":2.506,"Precursor.Area":28631778,"Apex.RT..min.":10.29,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KDELTLEGIK","Annotated.Sequence":"[K].KDELTLEGIK.[Q]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.3571,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":573.32351,"Contaminant":"False","Aligned.m.z..Da.":573.32351,"MH...Da.":1145.63974,"Theo..MH...Da.":1145.64123,"DeltaM..ppm.":-1.3,"Deltam.z..Da.":-0.00075,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":29138454,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":46.02014,"Ion.Inject.Time..ms.":22,"RT..min.":10.3103,"First.Scan":8916,"Last.Scan":8916,"Master.Scan.s.":8907,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":367,"XCorr":1.68,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":0.00105,"Percolator.SVMScore":0.944,"Precursor.Area":28631778,"Apex.RT..min.":10.29,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MFILDEADEMLSSGFK","Annotated.Sequence":"[K].MFILDEADEmLSSGFK.[E]","Modifications":"M10(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7244,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":924.92308,"Contaminant":"False","Aligned.m.z..Da.":924.92308,"MH...Da.":1848.83888,"Theo..MH...Da.":1848.83941,"DeltaM..ppm.":-0.29,"Deltam.z..Da.":-0.00027,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1736868.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":17.7383,"First.Scan":18506,"Last.Scan":18506,"Master.Scan.s.":18501,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":1794,"XCorr":3.81,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":4.024e-09,"Percolator.SVMScore":3.212,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MFILDEADEMLSSGFK","Annotated.Sequence":"[K].mFILDEADEMLSSGFK.[E]","Modifications":"M1(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.681,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":924.92382,"Contaminant":"False","Aligned.m.z..Da.":924.92382,"MH...Da.":1848.84037,"Theo..MH...Da.":1848.83941,"DeltaM..ppm.":0.51,"Deltam.z..Da.":0.00048,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1676305,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":4.48574,"Ion.Inject.Time..ms.":22,"RT..min.":18.253,"First.Scan":19169,"Last.Scan":19169,"Master.Scan.s.":19164,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":1737,"XCorr":3.95,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":5.619e-09,"Percolator.SVMScore":3.151,"Precursor.Area":1201921.125,"Apex.RT..min.":18.25,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MFILDEADEMLSSGFK","Annotated.Sequence":"[K].mFILDEADEMLSSGFK.[E]","Modifications":"M1(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.6529,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":616.9519,"Contaminant":"False","Aligned.m.z..Da.":616.9519,"MH...Da.":1848.84115,"Theo..MH...Da.":1848.83941,"DeltaM..ppm.":0.94,"Deltam.z..Da.":0.00058,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1449158.875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":3.98139,"Ion.Inject.Time..ms.":22,"RT..min.":18.2506,"First.Scan":19165,"Last.Scan":19165,"Master.Scan.s.":19164,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":1729,"XCorr":2.91,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":5.724e-06,"Percolator.SVMScore":1.893,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ELDEIAASLENL","Annotated.Sequence":"[K].ELDEIAASLENL.[-]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.671,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":658.83263,"Contaminant":"False","Aligned.m.z..Da.":658.83263,"MH...Da.":1316.65799,"Theo..MH...Da.":1316.658,"DeltaM..ppm.":-0.01,"Deltam.z..Da.":0,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3059570.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":18.4546,"First.Scan":19427,"Last.Scan":19427,"Master.Scan.s.":19424,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":702,"XCorr":3.07,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":0.00049,"Percolator.SVMScore":1.083,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DVSSFFESAIER","Annotated.Sequence":"[K].DVSSFFESAIER.[V]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q08421","Master.Protein.Descriptions":"Enhancer of translation termination 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ETT1 PE=1 SV=1","Protein.Accessions":"Q08421","Protein.Descriptions":"Enhancer of translation termination 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ETT1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6941,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":693.83035,"Contaminant":"False","Aligned.m.z..Da.":693.83035,"MH...Da.":1386.65343,"Theo..MH...Da.":1386.65359,"DeltaM..ppm.":-0.11,"Deltam.z..Da.":-0.00008,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1840180.875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":17.7377,"First.Scan":18505,"Last.Scan":18505,"Master.Scan.s.":18501,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":792,"XCorr":3.04,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":2.218e-06,"Percolator.SVMScore":2.066,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FLQNPLEIFVDDEAK","Annotated.Sequence":"[R].FLQNPLEIFVDDEAK.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6975,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":889.45296,"Contaminant":"False","Aligned.m.z..Da.":889.45296,"MH...Da.":1777.89865,"Theo..MH...Da.":1777.90069,"DeltaM..ppm.":-1.15,"Deltam.z..Da.":-0.00102,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":977557.375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":18.5344,"First.Scan":19530,"Last.Scan":19530,"Master.Scan.s.":19528,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":913,"XCorr":3.67,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":1.452e-09,"Percolator.SVMScore":3.397,"Precursor.Area":1507513.375,"Apex.RT..min.":18.55,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FLQNPLEIFVDDEAK","Annotated.Sequence":"[R].FLQNPLEIFVDDEAK.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.7412,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":593.30495,"Contaminant":"False","Aligned.m.z..Da.":593.30495,"MH...Da.":1777.90031,"Theo..MH...Da.":1777.90069,"DeltaM..ppm.":-0.22,"Deltam.z..Da.":-0.00013,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1604298.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":18.5562,"First.Scan":19558,"Last.Scan":19558,"Master.Scan.s.":19554,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":916,"XCorr":3.4,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":2.087e-06,"Percolator.SVMScore":2.077,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MFILDEADEMLSSGFK","Annotated.Sequence":"[K].MFILDEADEmLSSGFK.[E]","Modifications":"M10(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.47,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":616.95187,"Contaminant":"False","Aligned.m.z..Da.":616.95187,"MH...Da.":1848.84106,"Theo..MH...Da.":1848.83941,"DeltaM..ppm.":0.89,"Deltam.z..Da.":0.00055,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1087862.875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":17.81889,"Ion.Inject.Time..ms.":22,"RT..min.":17.742,"First.Scan":18512,"Last.Scan":18512,"Master.Scan.s.":18501,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":1742,"XCorr":2.17,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":0.00003,"Percolator.SVMScore":1.586,"Precursor.Area":739997.25,"Apex.RT..min.":17.76,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MFILDEADEMLSSGFK","Annotated.Sequence":"[K].MFILDEADEmLSSGFK.[E]","Modifications":"M10(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":3,"DeltaScore":0.6658,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":924.92428,"Contaminant":"False","Aligned.m.z..Da.":924.92428,"MH...Da.":1848.84128,"Theo..MH...Da.":1848.83941,"DeltaM..ppm.":1.01,"Deltam.z..Da.":0.00093,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1472416.125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":21.56414,"Ion.Inject.Time..ms.":22,"RT..min.":17.7761,"First.Scan":18554,"Last.Scan":18554,"Master.Scan.s.":18553,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":1729,"XCorr":3.98,"X..Protein.Groups":1,"Percolator.q.Value":0.00011,"Percolator.PEP":9.893e-11,"Percolator.SVMScore":3.885,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (P4)","Search.ID":"P","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LQETNPEEVPKFEK","Annotated.Sequence":"[K].LQETNPEEVPKFEK.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.5786,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":563.29035,"Contaminant":"False","Aligned.m.z..Da.":563.29035,"MH...Da.":1687.85651,"Theo..MH...Da.":1687.85374,"DeltaM..ppm.":1.64,"Deltam.z..Da.":0.00092,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2707980.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":39.83337,"Ion.Inject.Time..ms.":22,"RT..min.":9.3105,"First.Scan":7434,"Last.Scan":7434,"Master.Scan.s.":7427,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_03.raw","File.ID":"F21","Quan.Info":"","Peptides.Matched":846,"XCorr":1.59,"X..Protein.Groups":1,"Percolator.q.Value":0.00012,"Percolator.PEP":0.0001,"Percolator.SVMScore":1.411,"Precursor.Area":1674260.375,"Apex.RT..min.":9.3,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (P4)","Search.ID":"P","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"DFRHSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[K].DFRHSDsYsENETNHTNVPISSTGGTNNK.[T]","Modifications":"S7(Phospho); S9(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":1,"Charge":4,"Original.Precursor.Charge":4,"DeltaScore":0.0282,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":842.84184,"Contaminant":"False","Aligned.m.z..Da.":842.84196,"MH...Da.":3368.34556,"Theo..MH...Da.":3368.34888,"DeltaM..ppm.":-0.98,"Deltam.z..Da.":-0.00083,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":894381.8125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":14.19145,"Ion.Inject.Time..ms.":22,"RT..min.":8.6563,"First.Scan":6693,"Last.Scan":6693,"Master.Scan.s.":6686,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_03.raw","File.ID":"F21","Quan.Info":"","Peptides.Matched":918,"XCorr":6.73,"X..Protein.Groups":1,"Percolator.q.Value":0.00012,"Percolator.PEP":6.469e-10,"Percolator.SVMScore":3.576,"ptmRS..Binomial.Peptide.Score":306.98,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"b2+-H(3) O(4) P(8): 535.69; b2+- -2H(3) O(4) P -2H(3) O(4) P(17): 999.90; b2+- -2H(3) O(4) P -2H(3) O(4) P(18): 1049.44; b2+- -2H(3) O(4) P -2H(3) O(4) P(20): 1154.50","ptmRS..Best.Site.Probabilities":"S5(Phospho): 99.98; S7(Phospho): 98.57","ptmRS..Phospho.Site.Probabilities":"S(5): 100.0; S(7): 98.6; Y(8): 0.0; S(9): 1.5; T(13): 0.0; T(16): 0.0; S(21): 0.0; S(22): 0.0; T(23): 0.0; T(26): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (P4)","Search.ID":"P","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KDELTLEGIK","Annotated.Sequence":"[K].KDELTLEGIK.[Q]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.496,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":573.32397,"Contaminant":"False","Aligned.m.z..Da.":573.32401,"MH...Da.":1145.64068,"Theo..MH...Da.":1145.64123,"DeltaM..ppm.":-0.48,"Deltam.z..Da.":-0.00028,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3199523.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":4.17471,"Ion.Inject.Time..ms.":22,"RT..min.":10.2543,"First.Scan":8615,"Last.Scan":8615,"Master.Scan.s.":8610,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_03.raw","File.ID":"F21","Quan.Info":"","Peptides.Matched":314,"XCorr":3.79,"X..Protein.Groups":1,"Percolator.q.Value":0.00012,"Percolator.PEP":3.444e-08,"Percolator.SVMScore":2.853,"Precursor.Area":2646259,"Apex.RT..min.":10.26,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (P4)","Search.ID":"P","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KDELTLEGIK","Annotated.Sequence":"[K].KDELTLEGIK.[Q]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.5576,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":382.55162,"Contaminant":"False","Aligned.m.z..Da.":382.55165,"MH...Da.":1145.64033,"Theo..MH...Da.":1145.64123,"DeltaM..ppm.":-0.79,"Deltam.z..Da.":-0.0003,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1769758.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":47.63707,"Ion.Inject.Time..ms.":22,"RT..min.":10.2573,"First.Scan":8620,"Last.Scan":8620,"Master.Scan.s.":8610,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_03.raw","File.ID":"F21","Quan.Info":"","Peptides.Matched":351,"XCorr":2.78,"X..Protein.Groups":1,"Percolator.q.Value":0.00012,"Percolator.PEP":0.00004,"Percolator.SVMScore":1.571,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (P4)","Search.ID":"P","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"HSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[R].HSDsYsENETNHTNVPISSTGGTNNK.[T]","Modifications":"S4(Phospho); S6(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.1801,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":984.05538,"Contaminant":"False","Aligned.m.z..Da.":984.05555,"MH...Da.":2950.15158,"Theo..MH...Da.":2950.15241,"DeltaM..ppm.":-0.28,"Deltam.z..Da.":-0.00027,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2879412.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":8.031,"First.Scan":5979,"Last.Scan":5979,"Master.Scan.s.":5971,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_03.raw","File.ID":"F21","Quan.Info":"","Peptides.Matched":1113,"XCorr":6.72,"X..Protein.Groups":1,"Percolator.q.Value":0.00012,"Percolator.PEP":1.115e-11,"Percolator.SVMScore":4.314,"Precursor.Area":4519965,"Apex.RT..min.":8.04,"ptmRS..Binomial.Peptide.Score":643.78,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(4): 409.15; b1+-H(3) O(4) P(5): 572.21; b1+-H(3) O(4) P(6): 739.21; b1+-H(3) O(4) P(9): 1111.34; b1+-H(3) O(4) P(11): 1326.43; b1+-H(3) O(4) P(15): 1777.64; b2+-H(3) O(4) P(12): 732.25; b2+-H(3) O(4) P(14): 839.79; b2+-H(3) O(4) P(15): 889.33; y2+-H(3) O(4) P(23): 1257.03; b1+(2): 225.10; b1+(3): 340.13","ptmRS..Best.Site.Probabilities":"S4(Phospho): 100; S6(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(4): 100.0; Y(5): 0.0; S(6): 100.0; T(10): 0.0; T(13): 0.0; S(18): 0.0; S(19): 0.0; T(20): 0.0; T(23): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (P4)","Search.ID":"P","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"APQALMLAPTR","Annotated.Sequence":"[K].APQALmLAPTR.[E]","Modifications":"M6(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.742,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":592.82685,"Contaminant":"False","Aligned.m.z..Da.":592.82687,"MH...Da.":1184.64642,"Theo..MH...Da.":1184.6456,"DeltaM..ppm.":0.69,"Deltam.z..Da.":0.00041,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1422529.375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":16.08083,"Ion.Inject.Time..ms.":22,"RT..min.":10.178,"First.Scan":8517,"Last.Scan":8517,"Master.Scan.s.":8506,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_03.raw","File.ID":"F21","Quan.Info":"","Peptides.Matched":382,"XCorr":3.14,"X..Protein.Groups":1,"Percolator.q.Value":0.00012,"Percolator.PEP":3.615e-06,"Percolator.SVMScore":2.007,"Precursor.Area":2623466.25,"Apex.RT..min.":10.2,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (P4)","Search.ID":"P","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KFQDDTLNR","Annotated.Sequence":"[K].KFQDDtLNR.[V]","Modifications":"T6(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.686,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":608.77195,"Contaminant":"False","Aligned.m.z..Da.":608.77194,"MH...Da.":1216.53662,"Theo..MH...Da.":1216.53579,"DeltaM..ppm.":0.68,"Deltam.z..Da.":0.00041,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2482392.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":17.30579,"Ion.Inject.Time..ms.":22,"RT..min.":6.2084,"First.Scan":3128,"Last.Scan":3128,"Master.Scan.s.":3124,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_03.raw","File.ID":"F21","Quan.Info":"","Peptides.Matched":615,"XCorr":2.93,"X..Protein.Groups":1,"Percolator.q.Value":0.00012,"Percolator.PEP":0.00005,"Percolator.SVMScore":1.534,"Precursor.Area":2018379.625,"Apex.RT..min.":6.21,"ptmRS..Binomial.Peptide.Score":336.03,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"T6(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"T(6): 100.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (P4)","Search.ID":"P","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"AAEAGETGAATSATEGDNNNNTAAGDKK","Annotated.Sequence":"[K].AAEAGETGAATsATEGDNNNNTAAGDKK.[G]","Modifications":"S12(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0139,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":906.04964,"Contaminant":"False","Aligned.m.z..Da.":906.04954,"MH...Da.":2716.13436,"Theo..MH...Da.":2716.13298,"DeltaM..ppm.":0.51,"Deltam.z..Da.":0.00046,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":4437102,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":2.57228,"Ion.Inject.Time..ms.":22,"RT..min.":5.0144,"First.Scan":1752,"Last.Scan":1752,"Master.Scan.s.":1746,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_03.raw","File.ID":"F21","Quan.Info":"","Peptides.Matched":2541,"XCorr":7.17,"X..Protein.Groups":1,"Percolator.q.Value":0.00012,"Percolator.PEP":6.305e-16,"Percolator.SVMScore":7.341,"ptmRS..Binomial.Peptide.Score":462.58,"ptmRS..Isoform.Confidence.Probability":0.992,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(15): 1300.57; y1+-H(3) O(4) P(17): 1688.75; y2+-H(3) O(4) P(18): 895.40; y2+-H(3) O(4) P(17): 844.88; y2+(21): 1043.94","ptmRS..Best.Site.Probabilities":"S12(Phospho): 99.17","ptmRS..Phospho.Site.Probabilities":"T(7): 0.8; T(11): 0.0; S(12): 99.2; T(14): 0.0; T(22): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (P4)","Search.ID":"P","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"AAEAGETGAATSATEGDNNNNTAAGDKK","Annotated.Sequence":"[K].AAEAGETGAATSATEGDNNNNTAAGDKK.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.7744,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":879.3933,"Contaminant":"False","Aligned.m.z..Da.":879.39318,"MH...Da.":2636.16536,"Theo..MH...Da.":2636.16665,"DeltaM..ppm.":-0.49,"Deltam.z..Da.":-0.00043,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3156116.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":3.90411,"Ion.Inject.Time..ms.":22,"RT..min.":4.9316,"First.Scan":1680,"Last.Scan":1680,"Master.Scan.s.":1668,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_03.raw","File.ID":"F21","Quan.Info":"","Peptides.Matched":3672,"XCorr":5.23,"X..Protein.Groups":1,"Percolator.q.Value":0.00012,"Percolator.PEP":6.305e-16,"Percolator.SVMScore":8.421,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (P4)","Search.ID":"P","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"HGIVEEK","Annotated.Sequence":"[K].HGIVEEK.[I]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.4661,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":406.219,"Contaminant":"False","Aligned.m.z..Da.":406.21887,"MH...Da.":811.43072,"Theo..MH...Da.":811.43084,"DeltaM..ppm.":-0.16,"Deltam.z..Da.":-0.00006,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3775862,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":39.91294,"Ion.Inject.Time..ms.":12.157,"RT..min.":4.4638,"First.Scan":1254,"Last.Scan":1254,"Master.Scan.s.":1252,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_03.raw","File.ID":"F21","Quan.Info":"","Peptides.Matched":184,"XCorr":2.21,"X..Protein.Groups":1,"Percolator.q.Value":0.00012,"Percolator.PEP":0.0003,"Percolator.SVMScore":1.201,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (P4)","Search.ID":"P","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"LEAGLSDSK","Annotated.Sequence":"[K].LEAGLsDSK.[Q]","Modifications":"S6(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.0174,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":500.22403,"Contaminant":"False","Aligned.m.z..Da.":500.22408,"MH...Da.":999.44078,"Theo..MH...Da.":999.43943,"DeltaM..ppm.":1.35,"Deltam.z..Da.":0.00067,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2255788,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":22.11507,"Ion.Inject.Time..ms.":22,"RT..min.":7.5751,"First.Scan":5365,"Last.Scan":5365,"Master.Scan.s.":5360,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_03.raw","File.ID":"F21","Quan.Info":"","Peptides.Matched":749,"XCorr":2.3,"X..Protein.Groups":1,"Percolator.q.Value":0.00012,"Percolator.PEP":0.00073,"Percolator.SVMScore":1.042,"Precursor.Area":2844714,"Apex.RT..min.":7.59,"ptmRS..Binomial.Peptide.Score":249.11,"ptmRS..Isoform.Confidence.Probability":0.995,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(8): 788.38; y1+-H(3) O(4) P(7): 659.34; y1+-H(3) O(4) P(6): 588.30; y1+-H(3) O(4) P(5): 531.28; y1+-H(3) O(4) P(4): 418.19; y2+-H(3) O(4) P(6): 294.65; y1+(3): 349.17; y1+(2): 234.14","ptmRS..Best.Site.Probabilities":"S6(Phospho): 99.54","ptmRS..Phospho.Site.Probabilities":"S(6): 99.5; S(8): 0.5"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (P4)","Search.ID":"P","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"IGNNELLHR","Annotated.Sequence":"[K].IGNNELLHR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q03558","Master.Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","Protein.Accessions":"Q03558","Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.4167,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":355.86494,"Contaminant":"False","Aligned.m.z..Da.":355.86498,"MH...Da.":1065.58028,"Theo..MH...Da.":1065.57997,"DeltaM..ppm.":0.29,"Deltam.z..Da.":0.0001,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2097638.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":9.05718,"Ion.Inject.Time..ms.":22,"RT..min.":7.5756,"First.Scan":5366,"Last.Scan":5366,"Master.Scan.s.":5360,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_03.raw","File.ID":"F21","Quan.Info":"","Peptides.Matched":300,"XCorr":1.92,"X..Protein.Groups":1,"Percolator.q.Value":0.00012,"Percolator.PEP":0.00048,"Percolator.SVMScore":1.119,"Precursor.Area":1312480,"Apex.RT..min.":7.57,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (P4)","Search.ID":"P","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LQETNPEEVPK","Annotated.Sequence":"[K].LQETNPEEVPK.[F]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5401,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":642.3281,"Contaminant":"False","Aligned.m.z..Da.":642.32817,"MH...Da.":1283.64892,"Theo..MH...Da.":1283.64777,"DeltaM..ppm.":0.89,"Deltam.z..Da.":0.00057,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2037641,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":11.02462,"Ion.Inject.Time..ms.":22,"RT..min.":7.6314,"First.Scan":5448,"Last.Scan":5448,"Master.Scan.s.":5438,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_03.raw","File.ID":"F21","Quan.Info":"","Peptides.Matched":542,"XCorr":2.74,"X..Protein.Groups":1,"Percolator.q.Value":0.00012,"Percolator.PEP":0.00001,"Percolator.SVMScore":1.785,"Precursor.Area":6364118.5,"Apex.RT..min.":7.65,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (P4)","Search.ID":"P","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"HSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[R].HSDSYsENETNHTNVPISSTGGTNNK.[T]","Modifications":"S6(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0208,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":957.39962,"Contaminant":"False","Aligned.m.z..Da.":957.39974,"MH...Da.":2870.18432,"Theo..MH...Da.":2870.18608,"DeltaM..ppm.":-0.61,"Deltam.z..Da.":-0.00058,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":7096893,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":18.645,"RT..min.":7.6454,"First.Scan":5467,"Last.Scan":5467,"Master.Scan.s.":5464,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_03.raw","File.ID":"F21","Quan.Info":"","Peptides.Matched":2461,"XCorr":7.2,"X..Protein.Groups":1,"Percolator.q.Value":0.00012,"Percolator.PEP":6.305e-16,"Percolator.SVMScore":6.62,"Precursor.Area":9601265,"Apex.RT..min.":7.65,"ptmRS..Binomial.Peptide.Score":583.53,"ptmRS..Isoform.Confidence.Probability":0.994,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(6): 659.24; b1+-H(3) O(4) P(11): 1246.46; b1+-H(3) O(4) P(12): 1383.52; b1+-H(3) O(4) P(15): 1697.68; b2+-H(3) O(4) P(12): 692.26; b2+-H(3) O(4) P(13): 742.79; b2+-H(3) O(4) P(14): 799.81; b2+-H(3) O(4) P(15): 849.34; y2+-H(3) O(4) P(25): 1318.08; y2+-H(3) O(4) P(24): 1274.56; y2+-H(3) O(4) P(23): 1217.05; y2+-H(3) O(4) P(21): 1092.00; b1+(4): 427.16","ptmRS..Best.Site.Probabilities":"S6(Phospho): 99.4","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(4): 0.6; Y(5): 0.0; S(6): 99.4; T(10): 0.0; T(13): 0.0; S(18): 0.0; S(19): 0.0; T(20): 0.0; T(23): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (P4)","Search.ID":"P","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"HSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[R].HSDSySENETNHTNVPISSTGGTNNK.[T]","Modifications":"Y5(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":0,"Charge":4,"Original.Precursor.Charge":4,"DeltaScore":0.0051,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":718.30198,"Contaminant":"False","Aligned.m.z..Da.":718.30206,"MH...Da.":2870.18608,"Theo..MH...Da.":2870.18608,"DeltaM..ppm.":0,"Deltam.z..Da.":0,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3361572.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0.847,"Ion.Inject.Time..ms.":21.589,"RT..min.":7.646,"First.Scan":5468,"Last.Scan":5468,"Master.Scan.s.":5464,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_03.raw","File.ID":"F21","Quan.Info":"","Peptides.Matched":2501,"XCorr":5.86,"X..Protein.Groups":1,"Percolator.q.Value":0.00012,"Percolator.PEP":1.6e-12,"Percolator.SVMScore":4.667,"Precursor.Area":5590661,"Apex.RT..min.":7.65,"ptmRS..Binomial.Peptide.Score":300.88,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(9): 1031.37; b1+-H(3) O(4) P(11): 1246.46; b1+-H(3) O(4) P(12): 1383.52; b1+-H(3) O(4) P(15): 1697.68; b2+-H(3) O(4) P(7): 394.65; b2+-H(3) O(4) P(8): 451.67; b2+-H(3) O(4) P(13): 742.79; b2+-H(3) O(4) P(14): 799.81; b2+-H(3) O(4) P(15): 849.34; b2+-H(3) O(4) P(17): 954.41; b1+(4): 427.16","ptmRS..Best.Site.Probabilities":"S6(Phospho): 99.38","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(4): 0.6; Y(5): 0.0; S(6): 99.4; T(10): 0.0; T(13): 0.0; S(18): 0.0; S(19): 0.0; T(20): 0.0; T(23): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (P4)","Search.ID":"P","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSSPLHRLSPTGK","Annotated.Sequence":"[R].mSsPLHRLsPTGK.[S]","Modifications":"M1(Oxidation); S3(Phospho); S9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.4015,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":793.84406,"Contaminant":"False","Aligned.m.z..Da.":793.84417,"MH...Da.":1586.68085,"Theo..MH...Da.":1586.67977,"DeltaM..ppm.":0.68,"Deltam.z..Da.":0.00054,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2851276.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":5.7603,"Ion.Inject.Time..ms.":22,"RT..min.":7.7307,"First.Scan":5588,"Last.Scan":5588,"Master.Scan.s.":5581,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_03.raw","File.ID":"F21","Quan.Info":"","Peptides.Matched":1096,"XCorr":2.59,"X..Protein.Groups":1,"Percolator.q.Value":0.00012,"Percolator.PEP":0.00019,"Percolator.SVMScore":1.291,"Precursor.Area":2110123.75,"Apex.RT..min.":7.73,"ptmRS..Binomial.Peptide.Score":299.19,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(3): 304.10; y2+-H(3) O(4) P(11): 627.82; y2+- -2H(3) O(4) P -2H(3) O(4) P(11): 578.83; b1+(2): 235.07","ptmRS..Best.Site.Probabilities":"S3(Phospho): 100; S9(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(3): 100.0; S(9): 100.0; T(11): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (P4)","Search.ID":"P","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"IQDPQMTGYVSTR","Annotated.Sequence":"[R].IQDPQmtGYVsTR.[Y]","Modifications":"M6(Oxidation); T7(Phospho); S11(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P32485","Master.Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","Protein.Accessions":"P32485","Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.0479,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":836.32921,"Contaminant":"False","Aligned.m.z..Da.":836.32933,"MH...Da.":1671.65115,"Theo..MH...Da.":1671.64853,"DeltaM..ppm.":1.57,"Deltam.z..Da.":0.00131,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2464822.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":8.3141,"Ion.Inject.Time..ms.":22,"RT..min.":7.746,"First.Scan":5608,"Last.Scan":5608,"Master.Scan.s.":5607,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_03.raw","File.ID":"F21","Quan.Info":"","Peptides.Matched":748,"XCorr":3.13,"X..Protein.Groups":1,"Percolator.q.Value":0.00012,"Percolator.PEP":0.00002,"Percolator.SVMScore":1.726,"Precursor.Area":2176133.5,"Apex.RT..min.":7.74,"ptmRS..Binomial.Peptide.Score":361.85,"ptmRS..Isoform.Confidence.Probability":0.004,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(11): 1332.53; y1+-H(3) O(4) P(10): 1217.50; y1+-H(3) O(4) P(8): 992.39; y1+-H(3) O(4) P(7): 845.36; y2+-H(3) O(4) P(11): 666.77; y2+-H(3) O(4) P(10): 609.25; y1+(4): 462.27; y1+(3): 363.20","ptmRS..Best.Site.Probabilities":"T7(Phospho): 100; Y9(Phospho): 99.13","ptmRS..Phospho.Site.Probabilities":"T(7): 100.0; Y(9): 99.1; S(11): 0.4; T(12): 0.4"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (P4)","Search.ID":"P","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSSPLHRLSPTGK","Annotated.Sequence":"[R].mSsPLHRLsPTGK.[S]","Modifications":"M1(Oxidation); S3(Phospho); S9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.4435,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":529.56469,"Contaminant":"False","Aligned.m.z..Da.":529.56476,"MH...Da.":1586.67951,"Theo..MH...Da.":1586.67977,"DeltaM..ppm.":-0.16,"Deltam.z..Da.":-0.00009,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3389098.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":15.79928,"Ion.Inject.Time..ms.":22,"RT..min.":7.711,"First.Scan":5560,"Last.Scan":5560,"Master.Scan.s.":5555,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_03.raw","File.ID":"F21","Quan.Info":"","Peptides.Matched":988,"XCorr":3.63,"X..Protein.Groups":1,"Percolator.q.Value":0.00012,"Percolator.PEP":0.00008,"Percolator.SVMScore":1.436,"Precursor.Area":5013850,"Apex.RT..min.":7.73,"ptmRS..Binomial.Peptide.Score":302.8,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(8): 877.50; y1+-H(3) O(4) P(7): 740.44; y2+-H(3) O(4) P(12): 671.34; y2+-H(3) O(4) P(11): 627.82; y2+-H(3) O(4) P(10): 544.32; y2+-H(3) O(4) P(8): 439.25; y1+(4): 402.23; y1+(3): 305.18","ptmRS..Best.Site.Probabilities":"S3(Phospho): 100; S9(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(3): 100.0; S(9): 100.0; T(11): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (P4)","Search.ID":"P","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FFISNPDLVDRLEK","Annotated.Sequence":"[R].FFISNPDLVDRLEK.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q03558","Master.Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","Protein.Accessions":"Q03558","Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.6653,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":564.97057,"Contaminant":"False","Aligned.m.z..Da.":564.97069,"MH...Da.":1692.89716,"Theo..MH...Da.":1692.89555,"DeltaM..ppm.":0.96,"Deltam.z..Da.":0.00054,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":401872.3125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":44.69532,"Ion.Inject.Time..ms.":22,"RT..min.":15.3467,"First.Scan":15256,"Last.Scan":15256,"Master.Scan.s.":15254,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_03.raw","File.ID":"F21","Quan.Info":"","Peptides.Matched":587,"XCorr":2.39,"X..Protein.Groups":1,"Percolator.q.Value":0.00012,"Percolator.PEP":2.829e-07,"Percolator.SVMScore":2.471,"Precursor.Area":307878.3125,"Apex.RT..min.":15.34,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (P4)","Search.ID":"P","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.4638,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40728,"Contaminant":"False","Aligned.m.z..Da.":967.40749,"MH...Da.":2900.2073,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":2.75,"Deltam.z..Da.":0.00266,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":404622.96875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":14.9931,"First.Scan":14813,"Last.Scan":14813,"Master.Scan.s.":14812,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_03.raw","File.ID":"F21","Quan.Info":"","Peptides.Matched":2601,"XCorr":4.42,"X..Protein.Groups":1,"Percolator.q.Value":0.00012,"Percolator.PEP":2.691e-08,"Percolator.SVMScore":2.898,"Precursor.Area":574964.5,"Apex.RT..min.":14.83,"ptmRS..Binomial.Peptide.Score":352.47,"ptmRS..Isoform.Confidence.Probability":0.993,"ptmRS..Isoform.Group.Report":"y2+-H(3) O(4) P(14): 811.37","ptmRS..Best.Site.Probabilities":"S13(Phospho): 99.33","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.7; S(13): 99.3; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (P4)","Search.ID":"P","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FTVSAIYSDLPQQER","Annotated.Sequence":"[K].FTVSAIYSDLPQQER.[D]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7096,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":877.44044,"Contaminant":"False","Aligned.m.z..Da.":877.44064,"MH...Da.":1753.87361,"Theo..MH...Da.":1753.87554,"DeltaM..ppm.":-1.1,"Deltam.z..Da.":-0.00096,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":367522.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":42.6745,"Ion.Inject.Time..ms.":22,"RT..min.":15.0187,"First.Scan":14847,"Last.Scan":14847,"Master.Scan.s.":14838,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_03.raw","File.ID":"F21","Quan.Info":"","Peptides.Matched":1152,"XCorr":3.96,"X..Protein.Groups":1,"Percolator.q.Value":0.00012,"Percolator.PEP":4.384e-12,"Percolator.SVMScore":4.483,"Precursor.Area":762676,"Apex.RT..min.":15.04,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (P4)","Search.ID":"P","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"GVFGYGFEEPSAIQQR","Annotated.Sequence":"[R].GVFGYGFEEPSAIQQR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7088,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":892.93365,"Contaminant":"False","Aligned.m.z..Da.":892.93385,"MH...Da.":1784.86003,"Theo..MH...Da.":1784.86022,"DeltaM..ppm.":-0.11,"Deltam.z..Da.":-0.0001,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":614363.4375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":5.62758,"Ion.Inject.Time..ms.":22,"RT..min.":15.015,"First.Scan":14841,"Last.Scan":14841,"Master.Scan.s.":14838,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_03.raw","File.ID":"F21","Quan.Info":"","Peptides.Matched":1263,"XCorr":3.4,"X..Protein.Groups":1,"Percolator.q.Value":0.00012,"Percolator.PEP":1.313e-11,"Percolator.SVMScore":4.284,"Precursor.Area":1009179.1875,"Apex.RT..min.":15.03,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (P4)","Search.ID":"P","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"GVFGYGFEEPSAIQQR","Annotated.Sequence":"[R].GVFGYGFEEPSAIQQR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.508,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":595.62439,"Contaminant":"False","Aligned.m.z..Da.":595.62452,"MH...Da.":1784.85862,"Theo..MH...Da.":1784.86022,"DeltaM..ppm.":-0.9,"Deltam.z..Da.":-0.00053,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":344400.65625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":19.22688,"Ion.Inject.Time..ms.":22,"RT..min.":15.0411,"First.Scan":14876,"Last.Scan":14876,"Master.Scan.s.":14864,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_03.raw","File.ID":"F21","Quan.Info":"","Peptides.Matched":1270,"XCorr":1.87,"X..Protein.Groups":1,"Percolator.q.Value":0.00012,"Percolator.PEP":0.00045,"Percolator.SVMScore":1.131,"Precursor.Area":202835.23438,"Apex.RT..min.":15.04,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (P4)","Search.ID":"P","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":5,"DeltaScore":0.0971,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40498,"Contaminant":"False","Aligned.m.z..Da.":967.4052,"MH...Da.":2900.2004,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":0.37,"Deltam.z..Da.":0.00036,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":7567253.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":9.36912,"Ion.Inject.Time..ms.":15.157,"RT..min.":15.1497,"First.Scan":15011,"Last.Scan":15011,"Master.Scan.s.":15007,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_03.raw","File.ID":"F21","Quan.Info":"","Peptides.Matched":2484,"XCorr":7,"X..Protein.Groups":1,"Percolator.q.Value":0.00012,"Percolator.PEP":6.305e-16,"Percolator.SVMScore":7.181,"Precursor.Area":35032376,"Apex.RT..min.":15.1,"ptmRS..Binomial.Peptide.Score":758.48,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(13): 1413.58; b1+-H(3) O(4) P(14): 1528.61; b1+-H(3) O(4) P(15): 1642.65; b1+-H(3) O(4) P(16): 1771.69; y1+-H(3) O(4) P(15): 1750.78; y1+-H(3) O(4) P(14): 1621.73; y1+-H(3) O(4) P(13): 1458.67; b2+-H(3) O(4) P(16): 886.35; b2+-H(3) O(4) P(18): 1014.90; y2+-H(3) O(4) P(14): 811.37; b1+(12): 1344.56; y1+(13): 1556.65","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (P4)","Search.ID":"P","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ILISTDLLAR","Annotated.Sequence":"[R].ILISTDLLAR.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5217,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":557.84555,"Contaminant":"False","Aligned.m.z..Da.":557.84565,"MH...Da.":1114.68383,"Theo..MH...Da.":1114.68304,"DeltaM..ppm.":0.71,"Deltam.z..Da.":0.0004,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":301244.34375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":29.85799,"Ion.Inject.Time..ms.":22,"RT..min.":15.6462,"First.Scan":15632,"Last.Scan":15632,"Master.Scan.s.":15631,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_03.raw","File.ID":"F21","Quan.Info":"","Peptides.Matched":124,"XCorr":1.61,"X..Protein.Groups":1,"Percolator.q.Value":0.00012,"Percolator.PEP":0.0013,"Percolator.SVMScore":0.938,"Precursor.Area":1853995.625,"Apex.RT..min.":15.6,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (P4)","Search.ID":"P","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].msEGITDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S2(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":3,"DeltaScore":0.1479,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1096.46451,"Contaminant":"False","Aligned.m.z..Da.":1096.46467,"MH...Da.":2191.92175,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":-2.78,"Deltam.z..Da.":-0.00305,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":11750173,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":13.38317,"Ion.Inject.Time..ms.":22,"RT..min.":15.8047,"First.Scan":15833,"Last.Scan":15833,"Master.Scan.s.":15826,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_03.raw","File.ID":"F21","Quan.Info":"","Peptides.Matched":1873,"XCorr":4.26,"X..Protein.Groups":1,"Percolator.q.Value":0.00012,"Percolator.PEP":1.755e-08,"Percolator.SVMScore":2.976,"Precursor.Area":14967521,"Apex.RT..min.":15.78,"ptmRS..Binomial.Peptide.Score":406.73,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(2): 112.04; b1+-H(3) O(4) P(3): 241.08; b1+-H(3) O(4) P(4): 298.10; b1+-H(3) O(4) P(5): 411.19; b1+-H(3) O(4) P(6): 512.24; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+-H(3) O(4) P(9): 869.39; b1+-H(3) O(4) P(10): 998.43; b1+-H(3) O(4) P(11): 1085.46","ptmRS..Best.Site.Probabilities":"S2(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 100.0; T(6): 0.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (P4)","Search.ID":"P","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].mSEGItDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); T6(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1013,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1096.4689,"Contaminant":"False","Aligned.m.z..Da.":1096.46912,"MH...Da.":2191.93053,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":1.23,"Deltam.z..Da.":0.00134,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":432747.46875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":15.5258,"First.Scan":15482,"Last.Scan":15482,"Master.Scan.s.":15475,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_03.raw","File.ID":"F21","Quan.Info":"","Peptides.Matched":2069,"XCorr":4.64,"X..Protein.Groups":1,"Percolator.q.Value":0.00012,"Percolator.PEP":5.277e-12,"Percolator.SVMScore":4.45,"Precursor.Area":379377.5625,"Apex.RT..min.":15.54,"ptmRS..Binomial.Peptide.Score":463.79,"ptmRS..Isoform.Confidence.Probability":0.996,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(6): 512.24; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+-H(3) O(4) P(9): 869.39; b1+-H(3) O(4) P(10): 998.43; b1+(3): 259.09; b1+(4): 316.11; b1+(5): 429.20","ptmRS..Best.Site.Probabilities":"T6(Phospho): 99.65","ptmRS..Phospho.Site.Probabilities":"S(2): 0.4; T(6): 99.7; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (P4)","Search.ID":"P","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ILISTDLLAR","Annotated.Sequence":"[R].ILISTDLLAR.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5055,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":557.84561,"Contaminant":"False","Aligned.m.z..Da.":557.84572,"MH...Da.":1114.68395,"Theo..MH...Da.":1114.68304,"DeltaM..ppm.":0.82,"Deltam.z..Da.":0.00046,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1555449.375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":21.96337,"Ion.Inject.Time..ms.":22,"RT..min.":15.5855,"First.Scan":15556,"Last.Scan":15556,"Master.Scan.s.":15553,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_03.raw","File.ID":"F21","Quan.Info":"","Peptides.Matched":124,"XCorr":2.73,"X..Protein.Groups":1,"Percolator.q.Value":0.00012,"Percolator.PEP":0.00002,"Percolator.SVMScore":1.677,"Precursor.Area":1853995.625,"Apex.RT..min.":15.6,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (P4)","Search.ID":"P","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].mSEGItDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); T6(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.1534,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":731.31388,"Contaminant":"False","Aligned.m.z..Da.":731.31403,"MH...Da.":2191.9271,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":-0.34,"Deltam.z..Da.":-0.00025,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":352702.9375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":6.21267,"Ion.Inject.Time..ms.":22,"RT..min.":15.529,"First.Scan":15487,"Last.Scan":15487,"Master.Scan.s.":15475,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_03.raw","File.ID":"F21","Quan.Info":"","Peptides.Matched":1941,"XCorr":3.65,"X..Protein.Groups":1,"Percolator.q.Value":0.00012,"Percolator.PEP":9.203e-09,"Percolator.SVMScore":3.093,"Precursor.Area":322079.875,"Apex.RT..min.":15.54,"ptmRS..Binomial.Peptide.Score":351.21,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+-H(3) O(4) P(9): 869.39; b1+(2): 130.05; b1+(3): 259.09; b1+(4): 316.11; b1+(5): 429.20; b2+(3): 130.05","ptmRS..Best.Site.Probabilities":"T6(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; T(6): 100.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (P4)","Search.ID":"P","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FDDMELDENLLR","Annotated.Sequence":"[K].FDDmELDENLLR.[G]","Modifications":"M4(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5872,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":763.34428,"Contaminant":"False","Aligned.m.z..Da.":763.34439,"MH...Da.":1525.68128,"Theo..MH...Da.":1525.6839,"DeltaM..ppm.":-1.71,"Deltam.z..Da.":-0.00131,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1072395.625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":59.70609,"Ion.Inject.Time..ms.":22,"RT..min.":14.4739,"First.Scan":14165,"Last.Scan":14165,"Master.Scan.s.":14161,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_03.raw","File.ID":"F21","Quan.Info":"","Peptides.Matched":963,"XCorr":2.98,"X..Protein.Groups":1,"Percolator.q.Value":0.00012,"Percolator.PEP":7.264e-08,"Percolator.SVMScore":2.718,"Precursor.Area":1071230.75,"Apex.RT..min.":14.49,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (P4)","Search.ID":"P","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ELDEIAASLENL","Annotated.Sequence":"[K].ELDEIAAsLENL.[-]","Modifications":"S8(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7294,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":698.81573,"Contaminant":"False","Aligned.m.z..Da.":698.81581,"MH...Da.":1396.62419,"Theo..MH...Da.":1396.62433,"DeltaM..ppm.":-0.1,"Deltam.z..Da.":-0.00007,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1677995.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":3.44501,"Ion.Inject.Time..ms.":22,"RT..min.":18.2475,"First.Scan":18945,"Last.Scan":18945,"Master.Scan.s.":18942,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_03.raw","File.ID":"F21","Quan.Info":"","Peptides.Matched":893,"XCorr":3.03,"X..Protein.Groups":1,"Percolator.q.Value":0.00012,"Percolator.PEP":0.0002,"Percolator.SVMScore":1.282,"Precursor.Area":1129765.625,"Apex.RT..min.":18.25,"ptmRS..Binomial.Peptide.Score":229.95,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"S8(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(8): 100.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (P4)","Search.ID":"P","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.3358,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40563,"Contaminant":"False","Aligned.m.z..Da.":967.40543,"MH...Da.":2900.20233,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":1.03,"Deltam.z..Da.":0.001,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":143872.84375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":18.9636,"First.Scan":19873,"Last.Scan":19873,"Master.Scan.s.":19862,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_03.raw","File.ID":"F21","Quan.Info":"","Peptides.Matched":2546,"XCorr":2.71,"X..Protein.Groups":1,"Percolator.q.Value":0.00012,"Percolator.PEP":1.51e-06,"Percolator.SVMScore":2.166,"Precursor.Area":176983.42188,"Apex.RT..min.":19.16,"ptmRS..Binomial.Peptide.Score":284.21,"ptmRS..Isoform.Confidence.Probability":0.992,"ptmRS..Isoform.Group.Report":"y2+-H(3) O(4) P(14): 811.37","ptmRS..Best.Site.Probabilities":"S13(Phospho): 99.15","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.9; S(13): 99.2; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (P4)","Search.ID":"P","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].msEGITDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S2(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.096,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1096.46718,"Contaminant":"False","Aligned.m.z..Da.":1096.46716,"MH...Da.":2191.92708,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":-0.35,"Deltam.z..Da.":-0.00038,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":149819.28125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":81.97282,"Ion.Inject.Time..ms.":22,"RT..min.":16.3359,"First.Scan":16500,"Last.Scan":16500,"Master.Scan.s.":16498,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_03.raw","File.ID":"F21","Quan.Info":"","Peptides.Matched":1942,"XCorr":1.77,"X..Protein.Groups":1,"Percolator.q.Value":0.00012,"Percolator.PEP":0.00004,"Percolator.SVMScore":1.56,"Precursor.Area":14967521,"Apex.RT..min.":15.78,"ptmRS..Binomial.Peptide.Score":222.47,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(2): 112.04; b1+-H(3) O(4) P(3): 241.08; b1+-H(3) O(4) P(4): 298.10; b1+-H(3) O(4) P(5): 411.19; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35","ptmRS..Best.Site.Probabilities":"S2(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 100.0; T(6): 0.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (P4)","Search.ID":"P","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEySDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); Y12(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.4179,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40703,"Contaminant":"False","Aligned.m.z..Da.":967.40711,"MH...Da.":2900.20654,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":2.49,"Deltam.z..Da.":0.0024,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":419321.34375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":16.0197,"First.Scan":16105,"Last.Scan":16105,"Master.Scan.s.":16099,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_03.raw","File.ID":"F21","Quan.Info":"","Peptides.Matched":2590,"XCorr":3.9,"X..Protein.Groups":1,"Percolator.q.Value":0.00012,"Percolator.PEP":5.197e-09,"Percolator.SVMScore":3.197,"ptmRS..Binomial.Peptide.Score":332.67,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"b2+-H(3) O(4) P(14): 764.81; y2+-H(3) O(4) P(14): 811.37; b1+(12): 1344.56","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (P4)","Search.ID":"P","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FDDMELDENLLR","Annotated.Sequence":"[K].FDDMELDENLLR.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5314,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":755.34873,"Contaminant":"False","Aligned.m.z..Da.":755.3488,"MH...Da.":1509.6902,"Theo..MH...Da.":1509.68898,"DeltaM..ppm.":0.8,"Deltam.z..Da.":0.00061,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":493655.59375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":36.53515,"Ion.Inject.Time..ms.":22,"RT..min.":16.0209,"First.Scan":16107,"Last.Scan":16107,"Master.Scan.s.":16099,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_03.raw","File.ID":"F21","Quan.Info":"","Peptides.Matched":1044,"XCorr":3.03,"X..Protein.Groups":1,"Percolator.q.Value":0.00012,"Percolator.PEP":2.469e-08,"Percolator.SVMScore":2.914,"Precursor.Area":545083.5,"Apex.RT..min.":16.03,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (P4)","Search.ID":"P","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].msEGITDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S2(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1379,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1096.46731,"Contaminant":"False","Aligned.m.z..Da.":1096.46737,"MH...Da.":2191.92734,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":-0.23,"Deltam.z..Da.":-0.00025,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":172360.8125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":11.9112,"Ion.Inject.Time..ms.":22,"RT..min.":16.1144,"First.Scan":16226,"Last.Scan":16226,"Master.Scan.s.":16216,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_03.raw","File.ID":"F21","Quan.Info":"","Peptides.Matched":1945,"XCorr":2.9,"X..Protein.Groups":1,"Percolator.q.Value":0.00012,"Percolator.PEP":3.945e-08,"Percolator.SVMScore":2.829,"Precursor.Area":14967521,"Apex.RT..min.":15.78,"ptmRS..Binomial.Peptide.Score":413.59,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(2): 112.04; b1+-H(3) O(4) P(3): 241.08; b1+-H(3) O(4) P(4): 298.10; b1+-H(3) O(4) P(5): 411.19; b1+-H(3) O(4) P(6): 512.24; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+-H(3) O(4) P(9): 869.39; b1+-H(3) O(4) P(10): 998.43","ptmRS..Best.Site.Probabilities":"S2(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 100.0; T(6): 0.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (P4)","Search.ID":"P","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DIFSNDELLSDAYDAK","Annotated.Sequence":"[K].DIFSNDELLSDAYDAK.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.6693,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":605.94743,"Contaminant":"False","Aligned.m.z..Da.":605.94728,"MH...Da.":1815.82774,"Theo..MH...Da.":1815.82831,"DeltaM..ppm.":-0.32,"Deltam.z..Da.":-0.00019,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":378721.78125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":7.20939,"Ion.Inject.Time..ms.":22,"RT..min.":17.086,"First.Scan":17455,"Last.Scan":17455,"Master.Scan.s.":17447,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_03.raw","File.ID":"F21","Quan.Info":"","Peptides.Matched":1633,"XCorr":2.54,"X..Protein.Groups":1,"Percolator.q.Value":0.00012,"Percolator.PEP":0.00012,"Percolator.SVMScore":1.366,"Precursor.Area":496192.03125,"Apex.RT..min.":17.1,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (P4)","Search.ID":"P","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0027,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40831,"Contaminant":"False","Aligned.m.z..Da.":967.40822,"MH...Da.":2900.21039,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":3.82,"Deltam.z..Da.":0.00369,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":257783.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":54.43733,"Ion.Inject.Time..ms.":22,"RT..min.":16.5328,"First.Scan":16753,"Last.Scan":16753,"Master.Scan.s.":16745,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_03.raw","File.ID":"F21","Quan.Info":"","Peptides.Matched":2657,"XCorr":3.73,"X..Protein.Groups":1,"Percolator.q.Value":0.00012,"Percolator.PEP":1.445e-08,"Percolator.SVMScore":3.011,"ptmRS..Binomial.Peptide.Score":292.26,"ptmRS..Isoform.Confidence.Probability":0.5,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"Y12(Phospho): 50; S13(Phospho): 50","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 50.0; S(13): 50.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (P4)","Search.ID":"P","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"APQALMLAPTR","Annotated.Sequence":"[K].APQALMLAPTR.[E]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6178,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":584.82887,"Contaminant":"False","Aligned.m.z..Da.":584.82897,"MH...Da.":1168.65047,"Theo..MH...Da.":1168.65069,"DeltaM..ppm.":-0.19,"Deltam.z..Da.":-0.00011,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":560076.875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":80.49864,"Ion.Inject.Time..ms.":22,"RT..min.":11.6222,"First.Scan":10431,"Last.Scan":10431,"Master.Scan.s.":10430,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_03.raw","File.ID":"F21","Quan.Info":"","Peptides.Matched":164,"XCorr":3.14,"X..Protein.Groups":1,"Percolator.q.Value":0.00012,"Percolator.PEP":1.421e-07,"Percolator.SVMScore":2.596,"Precursor.Area":580082.9375,"Apex.RT..min.":11.62,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (P4)","Search.ID":"P","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"APQALMLAPTR","Annotated.Sequence":"[K].APQALMLAPTR.[E]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":3,"DeltaScore":0.6124,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":584.82936,"Contaminant":"False","Aligned.m.z..Da.":584.82946,"MH...Da.":1168.65144,"Theo..MH...Da.":1168.65069,"DeltaM..ppm.":0.64,"Deltam.z..Da.":0.00038,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":465046.0625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":84.04124,"Ion.Inject.Time..ms.":22,"RT..min.":11.6454,"First.Scan":10464,"Last.Scan":10464,"Master.Scan.s.":10456,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_03.raw","File.ID":"F21","Quan.Info":"","Peptides.Matched":174,"XCorr":1.29,"X..Protein.Groups":1,"Percolator.q.Value":0.00012,"Percolator.PEP":0.00002,"Percolator.SVMScore":1.668,"Precursor.Area":580082.9375,"Apex.RT..min.":11.62,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (P4)","Search.ID":"P","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"TAVFYGGTPISK","Annotated.Sequence":"[K].TAVFYGGTPISK.[D]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.456,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":620.8303,"Contaminant":"False","Aligned.m.z..Da.":620.83041,"MH...Da.":1240.65332,"Theo..MH...Da.":1240.65721,"DeltaM..ppm.":-3.14,"Deltam.z..Da.":-0.00195,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":383131.28125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":84.57118,"Ion.Inject.Time..ms.":22,"RT..min.":11.1248,"First.Scan":9762,"Last.Scan":9762,"Master.Scan.s.":9754,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_03.raw","File.ID":"F21","Quan.Info":"","Peptides.Matched":410,"XCorr":2.5,"X..Protein.Groups":1,"Percolator.q.Value":0.00012,"Percolator.PEP":0.00028,"Percolator.SVMScore":1.216,"Precursor.Area":469377.84375,"Apex.RT..min.":11.14,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (P4)","Search.ID":"P","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MGSINNNFNTNNNSNTDLDRDWK","Annotated.Sequence":"[-].mGSINNNFNTNNNsNTDLDRDWK.[T]","Modifications":"N-Term(Prot)(Met-loss); S14(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P39517","Master.Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","Protein.Accessions":"P39517","Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0518,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":878.36981,"Contaminant":"False","Aligned.m.z..Da.":878.36998,"MH...Da.":2633.09489,"Theo..MH...Da.":2633.10123,"DeltaM..ppm.":-2.41,"Deltam.z..Da.":-0.00211,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":4815160,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":11.3246,"First.Scan":10030,"Last.Scan":10030,"Master.Scan.s.":10027,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_03.raw","File.ID":"F21","Quan.Info":"","Peptides.Matched":2149,"XCorr":5.79,"X..Protein.Groups":1,"Percolator.q.Value":0.00012,"Percolator.PEP":6.305e-16,"Percolator.SVMScore":6.59,"Precursor.Area":3522591,"Apex.RT..min.":11.32,"ptmRS..Binomial.Peptide.Score":594.34,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(11): 1345.61; y1+-H(3) O(4) P(10): 1231.57; y2+-H(3) O(4) P(19): 1139.50; y2+-H(3) O(4) P(18): 1082.48; y2+-H(3) O(4) P(17): 1025.45; y2+-H(3) O(4) P(16): 968.43; y2+-H(3) O(4) P(15): 894.90; y2+-H(3) O(4) P(14): 837.88; y2+-H(3) O(4) P(13): 787.35; y2+-H(3) O(4) P(12): 730.33; y2+-H(3) O(4) P(11): 673.31; y2+-H(3) O(4) P(10): 616.29; y1+(9): 1162.55; y1+(8): 1048.51","ptmRS..Best.Site.Probabilities":"S14(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(3): 0.0; T(10): 0.0; S(14): 100.0; T(16): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (P4)","Search.ID":"P","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"MGSINNNFNTNNNSNTDLDRDWK","Annotated.Sequence":"[-].mGSINNNFNTNNNsNTDLDRDWK.[T]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S14(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P39517","Master.Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","Protein.Accessions":"P39517","Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0183,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":892.37203,"Contaminant":"False","Aligned.m.z..Da.":892.37217,"MH...Da.":2675.10154,"Theo..MH...Da.":2675.11179,"DeltaM..ppm.":-3.83,"Deltam.z..Da.":-0.00342,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":211728.6875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":13.5313,"First.Scan":12970,"Last.Scan":12970,"Master.Scan.s.":12965,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_03.raw","File.ID":"F21","Quan.Info":"","Peptides.Matched":2002,"XCorr":3.27,"X..Protein.Groups":1,"Percolator.q.Value":0.00012,"Percolator.PEP":0.00001,"Percolator.SVMScore":1.751,"Precursor.Area":4599431,"Apex.RT..min.":13.57,"ptmRS..Binomial.Peptide.Score":317.52,"ptmRS..Isoform.Confidence.Probability":0.992,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(12): 1459.66; y1+-H(3) O(4) P(11): 1345.61; y1+-H(3) O(4) P(10): 1231.57; y2+-H(3) O(4) P(17): 1025.45; y2+-H(3) O(4) P(16): 968.43; y2+-H(3) O(4) P(15): 894.90; y2+-H(3) O(4) P(14): 837.88; y2+-H(3) O(4) P(13): 787.35; y2+-H(3) O(4) P(12): 730.33; y1+(9): 1162.55","ptmRS..Best.Site.Probabilities":"S14(Phospho): 99.17","ptmRS..Phospho.Site.Probabilities":"S(3): 0.0; T(10): 0.0; S(14): 99.2; T(16): 0.8"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (P4)","Search.ID":"P","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MGSINNNFNTNNNSNTDLDRDWK","Annotated.Sequence":"[-].mGSINNNFNTNNNsNTDLDRDWK.[T]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S14(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P39517","Master.Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","Protein.Accessions":"P39517","Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.0713,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1338.05403,"Contaminant":"False","Aligned.m.z..Da.":1338.05424,"MH...Da.":2675.10078,"Theo..MH...Da.":2675.11179,"DeltaM..ppm.":-4.12,"Deltam.z..Da.":-0.0055,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":482358.125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":13.5624,"First.Scan":13010,"Last.Scan":13010,"Master.Scan.s.":13004,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_03.raw","File.ID":"F21","Quan.Info":"","Peptides.Matched":1959,"XCorr":5.33,"X..Protein.Groups":1,"Percolator.q.Value":0.00012,"Percolator.PEP":8.67e-09,"Percolator.SVMScore":3.104,"Precursor.Area":537472.6875,"Apex.RT..min.":13.57,"ptmRS..Binomial.Peptide.Score":565.93,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(15): 1788.79; y1+-H(3) O(4) P(14): 1674.75; y1+-H(3) O(4) P(13): 1573.70; y1+-H(3) O(4) P(12): 1459.66; y1+-H(3) O(4) P(10): 1231.57; b2+-H(3) O(4) P(19): 987.41; y2+-H(3) O(4) P(19): 1139.50; y2+-H(3) O(4) P(18): 1082.48; y2+-H(3) O(4) P(17): 1025.45; y2+-H(3) O(4) P(15): 894.90; y1+(9): 1162.55; y1+(8): 1048.51","ptmRS..Best.Site.Probabilities":"S14(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(3): 0.0; T(10): 0.0; S(14): 100.0; T(16): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (P4)","Search.ID":"P","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"TGTFSIAALQR","Annotated.Sequence":"[K].TGTFSIAALQR.[I]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7737,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":582.8221,"Contaminant":"False","Aligned.m.z..Da.":582.8222,"MH...Da.":1164.63693,"Theo..MH...Da.":1164.63715,"DeltaM..ppm.":-0.19,"Deltam.z..Da.":-0.00011,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2079127,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":44.87201,"Ion.Inject.Time..ms.":22,"RT..min.":13.3671,"First.Scan":12759,"Last.Scan":12759,"Master.Scan.s.":12757,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_03.raw","File.ID":"F21","Quan.Info":"","Peptides.Matched":364,"XCorr":3.8,"X..Protein.Groups":1,"Percolator.q.Value":0.00012,"Percolator.PEP":6.434e-07,"Percolator.SVMScore":2.321,"Precursor.Area":1849704.875,"Apex.RT..min.":13.37,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (P4)","Search.ID":"P","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DFLLKPELSR","Annotated.Sequence":"[K].DFLLKPELSR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.6114,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":406.56782,"Contaminant":"False","Aligned.m.z..Da.":406.56788,"MH...Da.":1217.68891,"Theo..MH...Da.":1217.68885,"DeltaM..ppm.":0.05,"Deltam.z..Da.":0.00002,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":592132.8125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":6.43802,"Ion.Inject.Time..ms.":22,"RT..min.":13.1563,"First.Scan":12489,"Last.Scan":12489,"Master.Scan.s.":12484,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_03.raw","File.ID":"F21","Quan.Info":"","Peptides.Matched":265,"XCorr":2.29,"X..Protein.Groups":1,"Percolator.q.Value":0.00012,"Percolator.PEP":0.00002,"Percolator.SVMScore":1.713,"Precursor.Area":304382.5625,"Apex.RT..min.":13.16,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (P4)","Search.ID":"P","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"AIMPIIEGHDVLAQAQSGTGK","Annotated.Sequence":"[R].AImPIIEGHDVLAQAQSGTGK.[T]","Modifications":"M3(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.632,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":718.03948,"Contaminant":"False","Aligned.m.z..Da.":718.03958,"MH...Da.":2152.1039,"Theo..MH...Da.":2152.10668,"DeltaM..ppm.":-1.29,"Deltam.z..Da.":-0.00093,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":589642.875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":6.48333,"Ion.Inject.Time..ms.":22,"RT..min.":12.7606,"First.Scan":11970,"Last.Scan":11970,"Master.Scan.s.":11964,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_03.raw","File.ID":"F21","Quan.Info":"","Peptides.Matched":1020,"XCorr":4.62,"X..Protein.Groups":1,"Percolator.q.Value":0.00012,"Percolator.PEP":3.109e-12,"Percolator.SVMScore":4.546,"Precursor.Area":1087115.875,"Apex.RT..min.":12.78,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AC4)","Search.ID":"AC","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MGSINNNFNTNNNSNTDLDRDWK","Annotated.Sequence":"[-].mGSINNNFNTNNNsNTDLDRDWK.[T]","Modifications":"N-Term(Prot)(Met-loss); S14(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P39517","Master.Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","Protein.Accessions":"P39517","Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0386,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":878.37229,"Contaminant":"False","Aligned.m.z..Da.":878.37228,"MH...Da.":2633.10232,"Theo..MH...Da.":2633.10123,"DeltaM..ppm.":0.41,"Deltam.z..Da.":0.00036,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1299661.125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":5.25872,"Ion.Inject.Time..ms.":22,"RT..min.":11.3109,"First.Scan":9971,"Last.Scan":9971,"Master.Scan.s.":9967,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_02.raw","File.ID":"F8","Quan.Info":"","Peptides.Matched":2275,"XCorr":4.15,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":3.163e-13,"Percolator.SVMScore":4.196,"Precursor.Area":1742214.125,"Apex.RT..min.":11.32,"ptmRS..Binomial.Peptide.Score":459.56,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(12): 1459.66; y1+-H(3) O(4) P(11): 1345.61; y1+-H(3) O(4) P(10): 1231.57; y2+-H(3) O(4) P(17): 1025.45; y2+-H(3) O(4) P(16): 968.43; y2+-H(3) O(4) P(15): 894.90; y2+-H(3) O(4) P(14): 837.88; y2+-H(3) O(4) P(13): 787.35; y2+-H(3) O(4) P(12): 730.33; y2+-H(3) O(4) P(11): 673.31; y2+-H(3) O(4) P(10): 616.29; y1+(9): 1162.55; y2+(9): 581.78","ptmRS..Best.Site.Probabilities":"S14(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(3): 0.0; T(10): 0.0; S(14): 100.0; T(16): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AC4)","Search.ID":"AC","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KDELTLEGIK","Annotated.Sequence":"[K].KDELTLEGIK.[Q]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.505,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":573.32498,"Contaminant":"False","Aligned.m.z..Da.":573.32491,"MH...Da.":1145.64268,"Theo..MH...Da.":1145.64123,"DeltaM..ppm.":1.27,"Deltam.z..Da.":0.00072,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":819299.1875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":20.38336,"Ion.Inject.Time..ms.":22,"RT..min.":10.2697,"First.Scan":8614,"Last.Scan":8614,"Master.Scan.s.":8602,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_02.raw","File.ID":"F8","Quan.Info":"","Peptides.Matched":301,"XCorr":3.01,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":2.333e-08,"Percolator.SVMScore":2.478,"Precursor.Area":578417.25,"Apex.RT..min.":10.26,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AC4)","Search.ID":"AC","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KDELTLEGIK","Annotated.Sequence":"[K].KDELTLEGIK.[Q]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.5,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":382.55186,"Contaminant":"False","Aligned.m.z..Da.":382.55182,"MH...Da.":1145.64105,"Theo..MH...Da.":1145.64123,"DeltaM..ppm.":-0.16,"Deltam.z..Da.":-0.00006,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":992039.375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":48.75964,"Ion.Inject.Time..ms.":22,"RT..min.":10.2667,"First.Scan":8609,"Last.Scan":8609,"Master.Scan.s.":8602,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_02.raw","File.ID":"F8","Quan.Info":"","Peptides.Matched":311,"XCorr":2.48,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":0.00002,"Percolator.SVMScore":1.409,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AC4)","Search.ID":"AC","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"APQALMLAPTR","Annotated.Sequence":"[K].APQALmLAPTR.[E]","Modifications":"M6(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7026,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":592.82668,"Contaminant":"False","Aligned.m.z..Da.":592.82662,"MH...Da.":1184.64609,"Theo..MH...Da.":1184.6456,"DeltaM..ppm.":0.41,"Deltam.z..Da.":0.00024,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":930566.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":17.49501,"Ion.Inject.Time..ms.":22,"RT..min.":10.1897,"First.Scan":8509,"Last.Scan":8509,"Master.Scan.s.":8498,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_02.raw","File.ID":"F8","Quan.Info":"","Peptides.Matched":304,"XCorr":2.69,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":1.034e-06,"Percolator.SVMScore":1.897,"Precursor.Area":854380.9375,"Apex.RT..min.":10.19,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AC4)","Search.ID":"AC","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ELALQIQK","Annotated.Sequence":"[R].ELALQIQK.[V]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.2342,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":471.78476,"Contaminant":"False","Aligned.m.z..Da.":471.78472,"MH...Da.":942.56224,"Theo..MH...Da.":942.56186,"DeltaM..ppm.":0.41,"Deltam.z..Da.":0.00019,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":911683.9375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":9.70995,"Ion.Inject.Time..ms.":22,"RT..min.":10.5819,"First.Scan":9021,"Last.Scan":9021,"Master.Scan.s.":9018,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_02.raw","File.ID":"F8","Quan.Info":"","Peptides.Matched":292,"XCorr":2.22,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":0.00056,"Percolator.SVMScore":0.931,"Precursor.Area":746449.5,"Apex.RT..min.":10.59,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AC4)","Search.ID":"AC","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.3958,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40526,"Contaminant":"False","Aligned.m.z..Da.":967.40538,"MH...Da.":2900.20124,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":0.66,"Deltam.z..Da.":0.00064,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":229593.20312,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":15.0284,"First.Scan":14574,"Last.Scan":14574,"Master.Scan.s.":14564,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_02.raw","File.ID":"F8","Quan.Info":"","Peptides.Matched":2512,"XCorr":3.84,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":3.298e-09,"Percolator.SVMScore":2.778,"Precursor.Area":8138823.5,"Apex.RT..min.":15.09,"ptmRS..Binomial.Peptide.Score":320.95,"ptmRS..Isoform.Confidence.Probability":0.5,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"Y12(Phospho): 50; S13(Phospho): 50","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 50.0; S(13): 50.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AC4)","Search.ID":"AC","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FTVSAIYSDLPQQER","Annotated.Sequence":"[K].FTVSAIYSDLPQQER.[D]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6568,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":877.44209,"Contaminant":"False","Aligned.m.z..Da.":877.4422,"MH...Da.":1753.87691,"Theo..MH...Da.":1753.87554,"DeltaM..ppm.":0.78,"Deltam.z..Da.":0.00069,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":311434.4375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":19.51974,"Ion.Inject.Time..ms.":22,"RT..min.":15.0264,"First.Scan":14571,"Last.Scan":14571,"Master.Scan.s.":14564,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_02.raw","File.ID":"F8","Quan.Info":"","Peptides.Matched":1080,"XCorr":3.38,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":2.363e-10,"Percolator.SVMScore":3.182,"Precursor.Area":210918.84375,"Apex.RT..min.":15.03,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AC4)","Search.ID":"AC","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"GVFGYGFEEPSAIQQR","Annotated.Sequence":"[R].GVFGYGFEEPSAIQQR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6667,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":892.93354,"Contaminant":"False","Aligned.m.z..Da.":892.93365,"MH...Da.":1784.85981,"Theo..MH...Da.":1784.86022,"DeltaM..ppm.":-0.23,"Deltam.z..Da.":-0.00021,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":375100.125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":15.0252,"First.Scan":14569,"Last.Scan":14569,"Master.Scan.s.":14564,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_02.raw","File.ID":"F8","Quan.Info":"","Peptides.Matched":1287,"XCorr":3.66,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":4.938e-11,"Percolator.SVMScore":3.422,"Precursor.Area":308160.875,"Apex.RT..min.":15.03,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AC4)","Search.ID":"AC","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":5,"DeltaScore":0.0724,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40437,"Contaminant":"False","Aligned.m.z..Da.":967.40448,"MH...Da.":2900.19855,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":-0.27,"Deltam.z..Da.":-0.00026,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":4791534,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":3.85118,"Ion.Inject.Time..ms.":22,"RT..min.":15.1097,"First.Scan":14674,"Last.Scan":14674,"Master.Scan.s.":14668,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_02.raw","File.ID":"F8","Quan.Info":"","Peptides.Matched":2444,"XCorr":6.91,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":6.305e-16,"Percolator.SVMScore":6.503,"Precursor.Area":8138823.5,"Apex.RT..min.":15.09,"ptmRS..Binomial.Peptide.Score":715.05,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(13): 1413.58; b1+-H(3) O(4) P(14): 1528.61; b1+-H(3) O(4) P(15): 1642.65; b1+-H(3) O(4) P(17): 1899.75; y1+-H(3) O(4) P(15): 1750.78; y1+-H(3) O(4) P(14): 1621.73; y1+-H(3) O(4) P(13): 1458.67; b2+-H(3) O(4) P(16): 886.35; b2+-H(3) O(4) P(18): 1014.90; y2+-H(3) O(4) P(14): 811.37; b1+(12): 1344.56; y1+(13): 1556.65","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AC4)","Search.ID":"AC","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].msEGITDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S2(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":3,"DeltaScore":0.168,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1096.46432,"Contaminant":"False","Aligned.m.z..Da.":1096.46424,"MH...Da.":2191.92135,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":-2.96,"Deltam.z..Da.":-0.00324,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":69519.67188,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":44.92969,"Ion.Inject.Time..ms.":22,"RT..min.":15.9887,"First.Scan":15707,"Last.Scan":15707,"Master.Scan.s.":15705,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_02.raw","File.ID":"F8","Quan.Info":"","Peptides.Matched":1865,"XCorr":2.5,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":0.00004,"Percolator.SVMScore":1.335,"Precursor.Area":3845150.5,"Apex.RT..min.":15.78,"ptmRS..Binomial.Peptide.Score":279.93,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(2): 112.04; b1+-H(3) O(4) P(3): 241.08; b1+-H(3) O(4) P(4): 298.10; b1+-H(3) O(4) P(5): 411.19; b1+-H(3) O(4) P(6): 512.24; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35","ptmRS..Best.Site.Probabilities":"S2(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 100.0; T(6): 0.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AC4)","Search.ID":"AC","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.4249,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.4012,"Contaminant":"False","Aligned.m.z..Da.":967.4011,"MH...Da.":2900.18904,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":-3.55,"Deltam.z..Da.":-0.00343,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":214987.89062,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":16.0765,"First.Scan":15818,"Last.Scan":15818,"Master.Scan.s.":15808,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_02.raw","File.ID":"F8","Quan.Info":"","Peptides.Matched":2187,"XCorr":3.93,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":2.011e-08,"Percolator.SVMScore":2.501,"ptmRS..Binomial.Peptide.Score":312.34,"ptmRS..Isoform.Confidence.Probability":0.5,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"Y12(Phospho): 50; S13(Phospho): 50","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 50.0; S(13): 50.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AC4)","Search.ID":"AC","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FDDMELDENLLR","Annotated.Sequence":"[K].FDDMELDENLLR.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.55,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":755.34568,"Contaminant":"False","Aligned.m.z..Da.":755.34561,"MH...Da.":1509.68409,"Theo..MH...Da.":1509.68898,"DeltaM..ppm.":-3.24,"Deltam.z..Da.":-0.00245,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":346796.65625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":53.15981,"Ion.Inject.Time..ms.":22,"RT..min.":16.0557,"First.Scan":15792,"Last.Scan":15792,"Master.Scan.s.":15782,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_02.raw","File.ID":"F8","Quan.Info":"","Peptides.Matched":955,"XCorr":2.2,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":2.454e-06,"Percolator.SVMScore":1.764,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AC4)","Search.ID":"AC","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.395,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40357,"Contaminant":"False","Aligned.m.z..Da.":967.40362,"MH...Da.":2900.19616,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":-1.09,"Deltam.z..Da.":-0.00106,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":218682.92188,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":20.42676,"Ion.Inject.Time..ms.":22,"RT..min.":15.5433,"First.Scan":15194,"Last.Scan":15194,"Master.Scan.s.":15193,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_02.raw","File.ID":"F8","Quan.Info":"","Peptides.Matched":2331,"XCorr":4.38,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":4.72e-10,"Percolator.SVMScore":3.076,"Precursor.Area":1415734.25,"Apex.RT..min.":15.37,"ptmRS..Binomial.Peptide.Score":358.98,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(14): 1528.61; y2+-H(3) O(4) P(14): 811.37","ptmRS..Best.Site.Probabilities":"S13(Phospho): 99.99","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AC4)","Search.ID":"AC","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ILISTDLLAR","Annotated.Sequence":"[R].ILISTDLLAR.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5217,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":557.84555,"Contaminant":"False","Aligned.m.z..Da.":557.84557,"MH...Da.":1114.68383,"Theo..MH...Da.":1114.68304,"DeltaM..ppm.":0.71,"Deltam.z..Da.":0.0004,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":852947.8125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":24.67893,"Ion.Inject.Time..ms.":22,"RT..min.":15.6005,"First.Scan":15241,"Last.Scan":15241,"Master.Scan.s.":15231,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_02.raw","File.ID":"F8","Quan.Info":"","Peptides.Matched":124,"XCorr":2.53,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":0.00003,"Percolator.SVMScore":1.385,"Precursor.Area":616202.875,"Apex.RT..min.":15.6,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AC4)","Search.ID":"AC","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].msEGITDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S2(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1615,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1096.47295,"Contaminant":"False","Aligned.m.z..Da.":1096.47296,"MH...Da.":2191.93863,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":4.92,"Deltam.z..Da.":0.00539,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":183084.625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":15.7307,"First.Scan":15397,"Last.Scan":15397,"Master.Scan.s.":15390,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_02.raw","File.ID":"F8","Quan.Info":"","Peptides.Matched":2180,"XCorr":4.21,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":7.573e-12,"Percolator.SVMScore":3.709,"ptmRS..Binomial.Peptide.Score":505.73,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(2): 112.04; b1+-H(3) O(4) P(3): 241.08; b1+-H(3) O(4) P(4): 298.10; b1+-H(3) O(4) P(5): 411.19; b1+-H(3) O(4) P(6): 512.24; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+-H(3) O(4) P(9): 869.39; b1+-H(3) O(4) P(10): 998.43; b1+(5): 509.16; y1+(14): 1683.77","ptmRS..Best.Site.Probabilities":"S2(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 100.0; T(6): 0.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AC4)","Search.ID":"AC","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].msEGITDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S2(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0691,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":731.3153,"Contaminant":"False","Aligned.m.z..Da.":731.3153,"MH...Da.":2191.93136,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":1.6,"Deltam.z..Da.":0.00117,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":238642.42188,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":15.7456,"First.Scan":15414,"Last.Scan":15414,"Master.Scan.s.":15409,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_02.raw","File.ID":"F8","Quan.Info":"","Peptides.Matched":2047,"XCorr":3.33,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":4.321e-06,"Percolator.SVMScore":1.677,"Precursor.Area":1152175.375,"Apex.RT..min.":15.78,"ptmRS..Binomial.Peptide.Score":362.17,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(2): 112.04; b1+-H(3) O(4) P(3): 241.08; b1+-H(3) O(4) P(4): 298.10; b1+-H(3) O(4) P(5): 411.19; b1+-H(3) O(4) P(6): 512.24; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+-H(3) O(4) P(9): 869.39","ptmRS..Best.Site.Probabilities":"S2(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 100.0; T(6): 0.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AC4)","Search.ID":"AC","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"AIMPIIEGHDVLAQAQSGTGK","Annotated.Sequence":"[R].AImPIIEGHDVLAQAQSGTGK.[T]","Modifications":"M3(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.6741,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":718.03968,"Contaminant":"False","Aligned.m.z..Da.":718.03964,"MH...Da.":2152.10449,"Theo..MH...Da.":2152.10668,"DeltaM..ppm.":-1.02,"Deltam.z..Da.":-0.00073,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":483831.03125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":12.7737,"First.Scan":11924,"Last.Scan":11924,"Master.Scan.s.":11917,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_02.raw","File.ID":"F8","Quan.Info":"","Peptides.Matched":987,"XCorr":3.13,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":7.515e-10,"Percolator.SVMScore":3.004,"Precursor.Area":287136.8125,"Apex.RT..min.":12.77,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AC4)","Search.ID":"AC","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"AAEAGETGAATSATEGDNNNNTAAGDKK","Annotated.Sequence":"[K].AAEAGETGAATsATEGDNNNNTAAGDKK.[G]","Modifications":"S12(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0228,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":906.04957,"Contaminant":"False","Aligned.m.z..Da.":906.04955,"MH...Da.":2716.13417,"Theo..MH...Da.":2716.13298,"DeltaM..ppm.":0.44,"Deltam.z..Da.":0.0004,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2288754.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":5.0402,"First.Scan":1822,"Last.Scan":1822,"Master.Scan.s.":1816,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_02.raw","File.ID":"F8","Quan.Info":"","Peptides.Matched":2551,"XCorr":5.26,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":6.305e-16,"Percolator.SVMScore":6.067,"ptmRS..Binomial.Peptide.Score":490.27,"ptmRS..Isoform.Confidence.Probability":0.993,"ptmRS..Isoform.Group.Report":"y2+-H(3) O(4) P(21): 994.95; y2+-H(3) O(4) P(18): 895.40; y2+-H(3) O(4) P(17): 844.88","ptmRS..Best.Site.Probabilities":"S12(Phospho): 99.26","ptmRS..Phospho.Site.Probabilities":"T(7): 0.0; T(11): 0.7; S(12): 99.3; T(14): 0.0; T(22): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AC4)","Search.ID":"AC","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"IQDPQMTGYVSTR","Annotated.Sequence":"[R].IQDPQMTGyVSTR.[Y]","Modifications":"Y9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P32485","Master.Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","Protein.Accessions":"P32485","Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1196,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":788.34613,"Contaminant":"False","Aligned.m.z..Da.":788.34608,"MH...Da.":1575.68499,"Theo..MH...Da.":1575.68728,"DeltaM..ppm.":-1.46,"Deltam.z..Da.":-0.00115,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1764398.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":6.85226,"Ion.Inject.Time..ms.":22,"RT..min.":9.5714,"First.Scan":7706,"Last.Scan":7706,"Master.Scan.s.":7705,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_02.raw","File.ID":"F8","Quan.Info":"","Peptides.Matched":1104,"XCorr":3.01,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":1.948e-07,"Percolator.SVMScore":2.153,"Precursor.Area":1267823.25,"Apex.RT..min.":9.57,"ptmRS..Binomial.Peptide.Score":259.96,"ptmRS..Isoform.Confidence.Probability":0.002,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(11): 1236.57; y1+-H(3) O(4) P(10): 1121.54; y1+-H(3) O(4) P(7): 765.39; y1+(4): 462.27; y1+(3): 363.20","ptmRS..Best.Site.Probabilities":"T7(Phospho): 33.28; S11(Phospho): 33.28; T12(Phospho): 33.28","ptmRS..Phospho.Site.Probabilities":"T(7): 33.3; Y(9): 0.2; S(11): 33.3; T(12): 33.3"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AC4)","Search.ID":"AC","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"HSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[R].HSDSySENETNHTNVPISSTGGTNNK.[T]","Modifications":"Y5(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":0,"Charge":4,"Original.Precursor.Charge":4,"DeltaScore":0.0068,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":718.30238,"Contaminant":"False","Aligned.m.z..Da.":718.30237,"MH...Da.":2870.18771,"Theo..MH...Da.":2870.18608,"DeltaM..ppm.":0.57,"Deltam.z..Da.":0.00041,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":897060.6875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":7.6592,"First.Scan":5416,"Last.Scan":5416,"Master.Scan.s.":5404,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_02.raw","File.ID":"F8","Quan.Info":"","Peptides.Matched":2497,"XCorr":5.92,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":1.538e-12,"Percolator.SVMScore":3.953,"Precursor.Area":2017721.75,"Apex.RT..min.":7.67,"ptmRS..Binomial.Peptide.Score":285.44,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(11): 1246.46; b2+-H(3) O(4) P(7): 394.65; b2+-H(3) O(4) P(13): 742.79; b2+-H(3) O(4) P(14): 799.81; b2+-H(3) O(4) P(15): 849.34; b2+-H(3) O(4) P(17): 954.41; b1+(4): 427.16","ptmRS..Best.Site.Probabilities":"S6(Phospho): 99.26","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(4): 0.7; Y(5): 0.0; S(6): 99.3; T(10): 0.0; T(13): 0.0; S(18): 0.0; S(19): 0.0; T(20): 0.0; T(23): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AC4)","Search.ID":"AC","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LQETNPEEVPK","Annotated.Sequence":"[K].LQETNPEEVPK.[F]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5311,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":642.32856,"Contaminant":"False","Aligned.m.z..Da.":642.32854,"MH...Da.":1283.64984,"Theo..MH...Da.":1283.64777,"DeltaM..ppm.":1.61,"Deltam.z..Da.":0.00103,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2102107.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":7.6564,"First.Scan":5411,"Last.Scan":5411,"Master.Scan.s.":5404,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_02.raw","File.ID":"F8","Quan.Info":"","Peptides.Matched":511,"XCorr":2.73,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":0.00018,"Percolator.SVMScore":1.11,"Precursor.Area":2290480.5,"Apex.RT..min.":7.66,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AC4)","Search.ID":"AC","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"HSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[R].HSDSySENETNHTNVPISSTGGTNNK.[T]","Modifications":"Y5(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0079,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":957.40188,"Contaminant":"False","Aligned.m.z..Da.":957.40185,"MH...Da.":2870.19108,"Theo..MH...Da.":2870.18608,"DeltaM..ppm.":1.74,"Deltam.z..Da.":0.00167,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1594400.875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":7.6558,"First.Scan":5410,"Last.Scan":5410,"Master.Scan.s.":5404,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_02.raw","File.ID":"F8","Quan.Info":"","Peptides.Matched":2542,"XCorr":6.32,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":6.305e-16,"Percolator.SVMScore":5.646,"Precursor.Area":3627288.25,"Apex.RT..min.":7.66,"ptmRS..Binomial.Peptide.Score":289.16,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(4): 409.15; b1+-H(3) O(4) P(5): 572.21; b1+-H(3) O(4) P(6): 659.24; b1+-H(3) O(4) P(9): 1031.37; b1+-H(3) O(4) P(11): 1246.46; b1+-H(3) O(4) P(12): 1383.52; b2+-H(3) O(4) P(8): 451.67; b2+-H(3) O(4) P(12): 692.26; b2+-H(3) O(4) P(13): 742.79; b2+-H(3) O(4) P(14): 799.81; b2+-H(3) O(4) P(15): 849.34; y2+-H(3) O(4) P(25): 1318.08; y2+-H(3) O(4) P(24): 1274.56; y2+-H(3) O(4) P(23): 1217.05","ptmRS..Best.Site.Probabilities":"S4(Phospho): 99.37","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(4): 99.4; Y(5): 0.0; S(6): 0.6; T(10): 0.0; T(13): 0.0; S(18): 0.0; S(19): 0.0; T(20): 0.0; T(23): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AC4)","Search.ID":"AC","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSSPLHRLSPTGK","Annotated.Sequence":"[R].mSsPLHRLsPTGK.[S]","Modifications":"M1(Oxidation); S3(Phospho); S9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.4354,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":529.56518,"Contaminant":"False","Aligned.m.z..Da.":529.56518,"MH...Da.":1586.681,"Theo..MH...Da.":1586.67977,"DeltaM..ppm.":0.77,"Deltam.z..Da.":0.00041,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1982015.375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":12.59171,"Ion.Inject.Time..ms.":22,"RT..min.":7.7413,"First.Scan":5531,"Last.Scan":5531,"Master.Scan.s.":5521,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_02.raw","File.ID":"F8","Quan.Info":"","Peptides.Matched":1071,"XCorr":3.33,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":0.0002,"Percolator.SVMScore":1.088,"Precursor.Area":1994832.75,"Apex.RT..min.":7.74,"ptmRS..Binomial.Peptide.Score":267.95,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(8): 877.50; y1+-H(3) O(4) P(7): 740.44; y2+-H(3) O(4) P(12): 671.34; y2+-H(3) O(4) P(10): 544.32; y2+-H(3) O(4) P(9): 495.80; y2+-H(3) O(4) P(8): 439.25; y1+(4): 402.23; y1+(3): 305.18","ptmRS..Best.Site.Probabilities":"S3(Phospho): 100; S9(Phospho): 99.99","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(3): 100.0; S(9): 100.0; T(11): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AC4)","Search.ID":"AC","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DIFSNDELLSDAYDAK","Annotated.Sequence":"[K].DIFSNDELLSDAYDAK.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7117,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":908.41937,"Contaminant":"False","Aligned.m.z..Da.":908.41895,"MH...Da.":1815.83147,"Theo..MH...Da.":1815.82831,"DeltaM..ppm.":1.74,"Deltam.z..Da.":0.00158,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":527764.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":13.35874,"Ion.Inject.Time..ms.":22,"RT..min.":17.0694,"First.Scan":16936,"Last.Scan":16936,"Master.Scan.s.":16933,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_02.raw","File.ID":"F8","Quan.Info":"","Peptides.Matched":1696,"XCorr":3.85,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":6.221e-10,"Percolator.SVMScore":3.033,"Precursor.Area":848124.8125,"Apex.RT..min.":17.09,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AC4)","Search.ID":"AC","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DIFSNDELLSDAYDAK","Annotated.Sequence":"[K].DIFSNDELLSDAYDAK.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.6377,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":605.94802,"Contaminant":"False","Aligned.m.z..Da.":605.94773,"MH...Da.":1815.8295,"Theo..MH...Da.":1815.82831,"DeltaM..ppm.":0.65,"Deltam.z..Da.":0.0004,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":262388.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":17.0763,"First.Scan":16944,"Last.Scan":16944,"Master.Scan.s.":16938,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_02.raw","File.ID":"F8","Quan.Info":"","Peptides.Matched":1554,"XCorr":2.07,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":0.00015,"Percolator.SVMScore":1.133,"Precursor.Area":275798.875,"Apex.RT..min.":17.09,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (A4)","Search.ID":"A","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"HSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[R].HSDsYsENETNHTNVPISSTGGTNNK.[T]","Modifications":"S4(Phospho); S6(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.1436,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":984.05593,"Contaminant":"False","Aligned.m.z..Da.":984.05609,"MH...Da.":2950.15325,"Theo..MH...Da.":2950.15241,"DeltaM..ppm.":0.28,"Deltam.z..Da.":0.00028,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3123649.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":7.999,"First.Scan":5929,"Last.Scan":5929,"Master.Scan.s.":5922,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_03.raw","File.ID":"F36","Quan.Info":"","Peptides.Matched":1127,"XCorr":7.31,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":3.135e-11,"Percolator.SVMScore":4.636,"Precursor.Area":14651934,"Apex.RT..min.":8.01,"ptmRS..Binomial.Peptide.Score":665.66,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(6): 739.21; b1+-H(3) O(4) P(9): 1111.34; y2+-H(3) O(4) P(25): 1358.06; y2+-H(3) O(4) P(22): 1173.53; b1+- -2H(3) O(4) P -2H(3) O(4) P(6): 641.23; b1+- -2H(3) O(4) P -2H(3) O(4) P(11): 1228.45; b1+- -2H(3) O(4) P -2H(3) O(4) P(15): 1679.67; b2+- -2H(3) O(4) P -2H(3) O(4) P(13): 733.78; b2+- -2H(3) O(4) P -2H(3) O(4) P(14): 790.80; b2+- -2H(3) O(4) P -2H(3) O(4) P(15): 840.34","ptmRS..Best.Site.Probabilities":"S4(Phospho): 100; S6(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(4): 100.0; Y(5): 0.0; S(6): 100.0; T(10): 0.0; T(13): 0.0; S(18): 0.0; S(19): 0.0; T(20): 0.0; T(23): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (A4)","Search.ID":"A","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"HSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[R].HSDSySENETNHTNVPISSTGGTNNK.[T]","Modifications":"Y5(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":0,"Charge":4,"Original.Precursor.Charge":4,"DeltaScore":0.0032,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":718.2992,"Contaminant":"False","Aligned.m.z..Da.":718.29928,"MH...Da.":2870.17496,"Theo..MH...Da.":2870.18608,"DeltaM..ppm.":-3.87,"Deltam.z..Da.":-0.00278,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3647616.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":3.27601,"Ion.Inject.Time..ms.":22,"RT..min.":7.6039,"First.Scan":5419,"Last.Scan":5419,"Master.Scan.s.":5415,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_03.raw","File.ID":"F36","Quan.Info":"","Peptides.Matched":2190,"XCorr":6.2,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":1.42e-11,"Percolator.SVMScore":4.797,"Precursor.Area":12624837,"Apex.RT..min.":7.62,"ptmRS..Binomial.Peptide.Score":284.07,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(6): 659.24; b1+-H(3) O(4) P(9): 1031.37; b1+-H(3) O(4) P(11): 1246.46; b1+-H(3) O(4) P(12): 1383.52; b2+-H(3) O(4) P(7): 394.65; b2+-H(3) O(4) P(9): 516.19; b2+-H(3) O(4) P(13): 742.79; b2+-H(3) O(4) P(14): 799.81; b2+-H(3) O(4) P(15): 849.34; b2+-H(3) O(4) P(17): 954.41; b1+(4): 427.16","ptmRS..Best.Site.Probabilities":"S6(Phospho): 99.38","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(4): 0.6; Y(5): 0.0; S(6): 99.4; T(10): 0.0; T(13): 0.0; S(18): 0.0; S(19): 0.0; T(20): 0.0; T(23): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (A4)","Search.ID":"A","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"IQDPQMTGYVSTR","Annotated.Sequence":"[R].IQDPQmTGyVSTR.[Y]","Modifications":"M6(Oxidation); Y9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P32485","Master.Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","Protein.Accessions":"P32485","Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1093,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":796.34415,"Contaminant":"False","Aligned.m.z..Da.":796.34424,"MH...Da.":1591.68103,"Theo..MH...Da.":1591.6822,"DeltaM..ppm.":-0.74,"Deltam.z..Da.":-0.00059,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2602272.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":19.30787,"Ion.Inject.Time..ms.":22,"RT..min.":7.5778,"First.Scan":5387,"Last.Scan":5387,"Master.Scan.s.":5376,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_03.raw","File.ID":"F36","Quan.Info":"","Peptides.Matched":1200,"XCorr":3.11,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":1.824e-07,"Percolator.SVMScore":2.869,"Precursor.Area":14432895,"Apex.RT..min.":7.63,"ptmRS..Binomial.Peptide.Score":270.08,"ptmRS..Isoform.Confidence.Probability":0.991,"ptmRS..Isoform.Group.Report":"y1+(6): 762.32; y1+(5): 705.30","ptmRS..Best.Site.Probabilities":"Y9(Phospho): 99.08","ptmRS..Phospho.Site.Probabilities":"T(7): 0.5; Y(9): 99.1; S(11): 0.5; T(12): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (A4)","Search.ID":"A","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LEAGLSDSK","Annotated.Sequence":"[K].LEAGLsDSK.[Q]","Modifications":"S6(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.0886,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":500.22353,"Contaminant":"False","Aligned.m.z..Da.":500.22359,"MH...Da.":999.43979,"Theo..MH...Da.":999.43943,"DeltaM..ppm.":0.35,"Deltam.z..Da.":0.00018,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":8170093.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":11.48181,"Ion.Inject.Time..ms.":16.497,"RT..min.":7.563,"First.Scan":5366,"Last.Scan":5366,"Master.Scan.s.":5363,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_03.raw","File.ID":"F36","Quan.Info":"","Peptides.Matched":680,"XCorr":2.71,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":0.00004,"Percolator.SVMScore":1.752,"Precursor.Area":7378821.5,"Apex.RT..min.":7.57,"ptmRS..Binomial.Peptide.Score":273.65,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(8): 788.38; y1+-H(3) O(4) P(7): 659.34; y1+-H(3) O(4) P(6): 588.30; y1+-H(3) O(4) P(5): 531.28; y1+-H(3) O(4) P(4): 418.19; y2+-H(3) O(4) P(6): 294.65; y1+(3): 349.17; y1+(2): 234.14","ptmRS..Best.Site.Probabilities":"S6(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(6): 100.0; S(8): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (A4)","Search.ID":"A","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"HSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[R].HSDsYSENETNHTNVPISSTGGTNNK.[T]","Modifications":"S4(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.037,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":957.40176,"Contaminant":"False","Aligned.m.z..Da.":957.40187,"MH...Da.":2870.19073,"Theo..MH...Da.":2870.18608,"DeltaM..ppm.":1.62,"Deltam.z..Da.":0.00155,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1624604.625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":2.20958,"Ion.Inject.Time..ms.":22,"RT..min.":7.5376,"First.Scan":5334,"Last.Scan":5334,"Master.Scan.s.":5324,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_03.raw","File.ID":"F36","Quan.Info":"","Peptides.Matched":2548,"XCorr":5.14,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":6.423e-13,"Percolator.SVMScore":5.428,"Precursor.Area":24316514,"Apex.RT..min.":7.62,"ptmRS..Binomial.Peptide.Score":480.88,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(4): 409.15; b1+-H(3) O(4) P(5): 572.21; b1+-H(3) O(4) P(10): 1132.42; b2+-H(3) O(4) P(13): 742.79; b2+-H(3) O(4) P(14): 799.81; b2+-H(3) O(4) P(15): 849.34; y2+-H(3) O(4) P(24): 1274.56; y2+-H(3) O(4) P(23): 1217.05","ptmRS..Best.Site.Probabilities":"S4(Phospho): 99.99","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(4): 100.0; Y(5): 0.0; S(6): 0.0; T(10): 0.0; T(13): 0.0; S(18): 0.0; S(19): 0.0; T(20): 0.0; T(23): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (A4)","Search.ID":"A","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSSPLHRLSPTGK","Annotated.Sequence":"[R].mSsPLHRLsPTGK.[S]","Modifications":"M1(Oxidation); S3(Phospho); S9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.4312,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":793.84354,"Contaminant":"False","Aligned.m.z..Da.":793.84365,"MH...Da.":1586.67981,"Theo..MH...Da.":1586.67977,"DeltaM..ppm.":0.03,"Deltam.z..Da.":0.00002,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3351636.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":39.57919,"Ion.Inject.Time..ms.":22,"RT..min.":7.7146,"First.Scan":5564,"Last.Scan":5564,"Master.Scan.s.":5558,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_03.raw","File.ID":"F36","Quan.Info":"","Peptides.Matched":973,"XCorr":2.18,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":0.00005,"Percolator.SVMScore":1.709,"Precursor.Area":5882731.5,"Apex.RT..min.":7.74,"ptmRS..Binomial.Peptide.Score":308.06,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(8): 877.50; y1+-H(3) O(4) P(6): 584.34; y1+-H(3) O(4) P(5): 471.26; y2+-H(3) O(4) P(11): 627.82; y2+-H(3) O(4) P(10): 544.32; b1+- -2H(3) O(4) P -2H(3) O(4) P(9): 989.50; y1+(4): 402.23; y1+(3): 305.18","ptmRS..Best.Site.Probabilities":"S3(Phospho): 100; S9(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(3): 100.0; S(9): 100.0; T(11): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (A4)","Search.ID":"A","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSSPLHRLSPTGK","Annotated.Sequence":"[R].mSsPLHRLsPTGK.[S]","Modifications":"M1(Oxidation); S3(Phospho); S9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.4857,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":529.56483,"Contaminant":"False","Aligned.m.z..Da.":529.5649,"MH...Da.":1586.67994,"Theo..MH...Da.":1586.67977,"DeltaM..ppm.":0.1,"Deltam.z..Da.":0.00006,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3720931.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":7.26853,"Ion.Inject.Time..ms.":22,"RT..min.":7.6935,"First.Scan":5536,"Last.Scan":5536,"Master.Scan.s.":5532,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_03.raw","File.ID":"F36","Quan.Info":"","Peptides.Matched":975,"XCorr":3.5,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":0.00008,"Percolator.SVMScore":1.634,"Precursor.Area":16993440,"Apex.RT..min.":7.73,"ptmRS..Binomial.Peptide.Score":276.92,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(8): 877.50; y2+-H(3) O(4) P(12): 671.34; y2+-H(3) O(4) P(11): 627.82; y2+-H(3) O(4) P(10): 544.32; y2+-H(3) O(4) P(8): 439.25; y1+(4): 402.23; y1+(3): 305.18","ptmRS..Best.Site.Probabilities":"S3(Phospho): 100; S9(Phospho): 99.99","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(3): 100.0; S(9): 100.0; T(11): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (A4)","Search.ID":"A","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LQETNPEEVPK","Annotated.Sequence":"[K].LQETNPEEVPK.[F]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":3,"DeltaScore":0.3852,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":642.3276,"Contaminant":"False","Aligned.m.z..Da.":642.32768,"MH...Da.":1283.64793,"Theo..MH...Da.":1283.64777,"DeltaM..ppm.":0.12,"Deltam.z..Da.":0.00008,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3454897.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":56.03101,"Ion.Inject.Time..ms.":22,"RT..min.":7.6443,"First.Scan":5472,"Last.Scan":5472,"Master.Scan.s.":5467,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_03.raw","File.ID":"F36","Quan.Info":"","Peptides.Matched":526,"XCorr":2.44,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":0.00005,"Percolator.SVMScore":1.709,"Precursor.Area":2414244.75,"Apex.RT..min.":7.64,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (A4)","Search.ID":"A","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"IQDPQMTGYVSTR","Annotated.Sequence":"[R].IQDPQmtGYVsTR.[Y]","Modifications":"M6(Oxidation); T7(Phospho); S11(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P32485","Master.Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","Protein.Accessions":"P32485","Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.0448,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":836.32756,"Contaminant":"False","Aligned.m.z..Da.":836.32766,"MH...Da.":1671.64784,"Theo..MH...Da.":1671.64853,"DeltaM..ppm.":-0.41,"Deltam.z..Da.":-0.00035,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3260480.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":47.18821,"Ion.Inject.Time..ms.":22,"RT..min.":7.6356,"First.Scan":5461,"Last.Scan":5461,"Master.Scan.s.":5454,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_03.raw","File.ID":"F36","Quan.Info":"","Peptides.Matched":775,"XCorr":2.9,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":0.00013,"Percolator.SVMScore":1.529,"Precursor.Area":7707442.5,"Apex.RT..min.":7.67,"ptmRS..Binomial.Peptide.Score":327.7,"ptmRS..Isoform.Confidence.Probability":0.005,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(11): 1332.53; y1+-H(3) O(4) P(10): 1217.50; y1+-H(3) O(4) P(8): 992.39; y1+-H(3) O(4) P(7): 845.36; y2+-H(3) O(4) P(11): 666.77; y2+-H(3) O(4) P(10): 609.25; y1+(4): 462.27; y1+(3): 363.20","ptmRS..Best.Site.Probabilities":"T7(Phospho): 100; Y9(Phospho): 99.46","ptmRS..Phospho.Site.Probabilities":"T(7): 100.0; Y(9): 99.5; S(11): 0.5; T(12): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (A4)","Search.ID":"A","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LQETNPEEVPK","Annotated.Sequence":"[K].LQETNPEEVPK.[F]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.4531,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":642.32614,"Contaminant":"False","Aligned.m.z..Da.":642.32622,"MH...Da.":1283.64501,"Theo..MH...Da.":1283.64777,"DeltaM..ppm.":-2.15,"Deltam.z..Da.":-0.00138,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3344775.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":40.97859,"Ion.Inject.Time..ms.":22,"RT..min.":7.635,"First.Scan":5460,"Last.Scan":5460,"Master.Scan.s.":5454,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_03.raw","File.ID":"F36","Quan.Info":"","Peptides.Matched":640,"XCorr":2.45,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":0.0001,"Percolator.SVMScore":1.577,"Precursor.Area":2414244.75,"Apex.RT..min.":7.64,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (A4)","Search.ID":"A","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LREENFSSNTSELGNKK","Annotated.Sequence":"[K].LREENFSSNtSELGNKK.[H]","Modifications":"T10(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":2,"Charge":3,"Original.Precursor.Charge":2,"DeltaScore":0.09,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":678.31545,"Contaminant":"False","Aligned.m.z..Da.":678.31553,"MH...Da.":2032.93179,"Theo..MH...Da.":2032.93354,"DeltaM..ppm.":-0.86,"Deltam.z..Da.":-0.00058,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3279313,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":41.5726,"Ion.Inject.Time..ms.":22,"RT..min.":7.6282,"First.Scan":5452,"Last.Scan":5452,"Master.Scan.s.":5441,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_03.raw","File.ID":"F36","Quan.Info":"","Peptides.Matched":2138,"XCorr":3,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":0.00002,"Percolator.SVMScore":1.942,"ptmRS..Binomial.Peptide.Score":242.34,"ptmRS..Isoform.Confidence.Probability":0.99,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(9): 972.51; y1+-H(3) O(4) P(8): 858.47; y2+-H(3) O(4) P(15): 833.39; y2+-H(3) O(4) P(12): 647.33; y1+(7): 775.43","ptmRS..Best.Site.Probabilities":"T10(Phospho): 99.04","ptmRS..Phospho.Site.Probabilities":"S(7): 0.0; S(8): 0.0; T(10): 99.0; S(11): 1.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (A4)","Search.ID":"A","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"IQDPQMTGYVSTR","Annotated.Sequence":"[R].IQDPQmtGYVsTR.[Y]","Modifications":"M6(Oxidation); T7(Phospho); S11(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P32485","Master.Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","Protein.Accessions":"P32485","Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.0458,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":836.32798,"Contaminant":"False","Aligned.m.z..Da.":836.32808,"MH...Da.":1671.64868,"Theo..MH...Da.":1671.64853,"DeltaM..ppm.":0.09,"Deltam.z..Da.":0.00008,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":5086875.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":62.05515,"Ion.Inject.Time..ms.":22,"RT..min.":7.6461,"First.Scan":5475,"Last.Scan":5475,"Master.Scan.s.":5467,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_03.raw","File.ID":"F36","Quan.Info":"","Peptides.Matched":762,"XCorr":2.4,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":0.00002,"Percolator.SVMScore":1.929,"Precursor.Area":7707442.5,"Apex.RT..min.":7.67,"ptmRS..Binomial.Peptide.Score":310.26,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(11): 1332.53; y1+-H(3) O(4) P(10): 1217.50; y1+-H(3) O(4) P(8): 992.39; y1+-H(3) O(4) P(7): 845.36; y2+-H(3) O(4) P(11): 666.77; y2+-H(3) O(4) P(10): 609.25; y1+(4): 462.27; y1+(3): 363.20","ptmRS..Best.Site.Probabilities":"T7(Phospho): 100; Y9(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"T(7): 100.0; Y(9): 100.0; S(11): 0.0; T(12): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (A4)","Search.ID":"A","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSSPLHRLSPTGK","Annotated.Sequence":"[R].MSsPLHRLsPTGK.[S]","Modifications":"S3(Phospho); S9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.5229,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":524.23208,"Contaminant":"False","Aligned.m.z..Da.":524.23215,"MH...Da.":1570.68168,"Theo..MH...Da.":1570.68486,"DeltaM..ppm.":-2.02,"Deltam.z..Da.":-0.00106,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3044672.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":16.19973,"Ion.Inject.Time..ms.":22,"RT..min.":8.6598,"First.Scan":6748,"Last.Scan":6748,"Master.Scan.s.":6741,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_03.raw","File.ID":"F36","Quan.Info":"","Peptides.Matched":975,"XCorr":3.71,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":0.0002,"Percolator.SVMScore":1.442,"Precursor.Area":10312065,"Apex.RT..min.":8.69,"ptmRS..Binomial.Peptide.Score":252.06,"ptmRS..Isoform.Confidence.Probability":0.992,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(8): 877.50; y1+-H(3) O(4) P(7): 740.44; y2+-H(3) O(4) P(12): 671.34; y2+-H(3) O(4) P(10): 544.32; y2+-H(3) O(4) P(8): 439.25; y1+(4): 402.23","ptmRS..Best.Site.Probabilities":"S3(Phospho): 99.99; S9(Phospho): 99.18","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(3): 100.0; S(9): 99.2; T(11): 0.8"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (A4)","Search.ID":"A","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"LTESTVNHTNHSDDELPGHLLLDSGASR","Annotated.Sequence":"[K].LTESTVNHTNHsDDELPGHLLLDSGASR.[T]","Modifications":"S12(Phospho)","X..Proteins":15,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"Q99337; P0C2I9; P47100; P0C2J0; Q03619; P0C2I2; Q99231; Q12088; Q07793; Q04711; Q12316; O13535; Q04670; Q92393; Q12193","Protein.Descriptions":"Transposon Ty1-NL2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-NL2 PE=3 SV=1; Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-ER2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER2 PE=3 SV=1; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-LR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR1 PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-GR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR3 PE=3 SV=3; Transposon Ty1-H Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-H PE=3 SV=1; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-BR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-BR PE=3 SV=1","X..Missed.Cleavages":0,"Charge":4,"Original.Precursor.Charge":4,"DeltaScore":0.0162,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":774.6078,"Contaminant":"False","Aligned.m.z..Da.":774.60789,"MH...Da.":3095.40938,"Theo..MH...Da.":3095.40658,"DeltaM..ppm.":0.91,"Deltam.z..Da.":0.0007,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":863003.8125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":2.98534,"Ion.Inject.Time..ms.":22,"RT..min.":12.2754,"First.Scan":11335,"Last.Scan":11335,"Master.Scan.s.":11330,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_03.raw","File.ID":"F36","Quan.Info":"","Peptides.Matched":4653,"XCorr":4.33,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":4.826e-07,"Percolator.SVMScore":2.67,"Precursor.Area":1985735.75,"Apex.RT..min.":12.3,"ptmRS..Binomial.Peptide.Score":220.05,"ptmRS..Isoform.Confidence.Probability":0.013,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(2): 197.13; b1+-H(3) O(4) P(3): 326.17","ptmRS..Best.Site.Probabilities":"T2(Phospho): 94.99","ptmRS..Phospho.Site.Probabilities":"T(2): 95.0; S(4): 1.3; T(5): 1.3; T(9): 1.3; S(12): 1.3; S(24): 0.0; S(27): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (A4)","Search.ID":"A","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"DFRHSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[K].DFRHsDsYSENETNHTNVPISSTGGTNNK.[T]","Modifications":"S5(Phospho); S7(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0346,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1123.45423,"Contaminant":"False","Aligned.m.z..Da.":1123.45439,"MH...Da.":3368.34813,"Theo..MH...Da.":3368.34888,"DeltaM..ppm.":-0.22,"Deltam.z..Da.":-0.00025,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2860730.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":8.6699,"First.Scan":6760,"Last.Scan":6760,"Master.Scan.s.":6754,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_03.raw","File.ID":"F36","Quan.Info":"","Peptides.Matched":917,"XCorr":6.93,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":1.958e-12,"Percolator.SVMScore":5.201,"Precursor.Area":3249559.25,"Apex.RT..min.":8.67,"ptmRS..Binomial.Peptide.Score":526.43,"ptmRS..Isoform.Confidence.Probability":0.991,"ptmRS..Isoform.Group.Report":"y2+-H(3) O(4) P(24): 1274.56; b1+- -2H(3) O(4) P -2H(3) O(4) P(12): 1431.56; b1+- -2H(3) O(4) P -2H(3) O(4) P(14): 1646.65; b2+- -2H(3) O(4) P -2H(3) O(4) P(7): 405.17; b2+- -2H(3) O(4) P -2H(3) O(4) P(15): 892.36; b2+- -2H(3) O(4) P -2H(3) O(4) P(16): 942.88; b2+- -2H(3) O(4) P -2H(3) O(4) P(17): 999.90; b2+- -2H(3) O(4) P -2H(3) O(4) P(18): 1049.44","ptmRS..Best.Site.Probabilities":"S5(Phospho): 100; S7(Phospho): 99.06","ptmRS..Phospho.Site.Probabilities":"S(5): 100.0; S(7): 99.1; Y(8): 0.0; S(9): 0.9; T(13): 0.0; T(16): 0.0; S(21): 0.0; S(22): 0.0; T(23): 0.0; T(26): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (A4)","Search.ID":"A","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"AQERMSSPLHRLSPTGK","Annotated.Sequence":"[R].AQERmssPLHRLsPTGK.[S]","Modifications":"M5(Oxidation); S6(Phospho); S7(Phospho); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":2,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.4731,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":717.63258,"Contaminant":"False","Aligned.m.z..Da.":717.6327,"MH...Da.":2150.8832,"Theo..MH...Da.":2150.8855,"DeltaM..ppm.":-1.07,"Deltam.z..Da.":-0.00077,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2653563.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":75.45975,"Ion.Inject.Time..ms.":22,"RT..min.":8.057,"First.Scan":6001,"Last.Scan":6001,"Master.Scan.s.":6000,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_03.raw","File.ID":"F36","Quan.Info":"","Peptides.Matched":1357,"XCorr":3.34,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":0.00013,"Percolator.SVMScore":1.524,"Precursor.Area":1759433.625,"Apex.RT..min.":8.06,"ptmRS..Binomial.Peptide.Score":133.67,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y2+-H(3) O(4) P(10): 544.32; y2+-H(3) O(4) P(8): 439.25; b2+- -2H(3) O(4) P -2H(3) O(4) P(13): 777.36; y1+(4): 402.23; y1+(3): 305.18","ptmRS..Best.Site.Probabilities":"S6(Phospho): 100; S7(Phospho): 100; S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(6): 100.0; S(7): 100.0; S(13): 100.0; T(15): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (A4)","Search.ID":"A","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"NFASLEHSR","Annotated.Sequence":"[K].NFASLEHsR.[S]","Modifications":"S8(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.3571,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":570.74376,"Contaminant":"False","Aligned.m.z..Da.":570.74386,"MH...Da.":1140.48025,"Theo..MH...Da.":1140.48336,"DeltaM..ppm.":-2.73,"Deltam.z..Da.":-0.00156,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3326111.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":17.23355,"Ion.Inject.Time..ms.":21.34,"RT..min.":8.3187,"First.Scan":6328,"Last.Scan":6328,"Master.Scan.s.":6325,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_03.raw","File.ID":"F36","Quan.Info":"","Peptides.Matched":474,"XCorr":2.8,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":0.00131,"Percolator.SVMScore":1.044,"ptmRS..Binomial.Peptide.Score":295.7,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(8): 928.46; y1+-H(3) O(4) P(7): 781.40; y1+-H(3) O(4) P(6): 710.36; y1+-H(3) O(4) P(5): 623.33; y1+-H(3) O(4) P(4): 510.24; y1+-H(3) O(4) P(3): 381.20; y1+-H(3) O(4) P(2): 244.14; y2+-H(3) O(4) P(7): 391.20; y2+-H(3) O(4) P(6): 355.68; y1+(5): 721.30","ptmRS..Best.Site.Probabilities":"S8(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(4): 0.0; S(8): 100.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (A4)","Search.ID":"A","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"DFRHSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[K].DFRHSDSYsENETNHTNVPISSTGGTNNK.[T]","Modifications":"S9(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":1,"Charge":4,"Original.Precursor.Charge":4,"DeltaScore":0.0277,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":822.85043,"Contaminant":"False","Aligned.m.z..Da.":822.85057,"MH...Da.":3288.3799,"Theo..MH...Da.":3288.38255,"DeltaM..ppm.":-0.8,"Deltam.z..Da.":-0.00066,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1885725,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":22.29841,"Ion.Inject.Time..ms.":22,"RT..min.":8.3114,"First.Scan":6320,"Last.Scan":6320,"Master.Scan.s.":6312,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_03.raw","File.ID":"F36","Quan.Info":"","Peptides.Matched":2330,"XCorr":5.06,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":6.757e-11,"Percolator.SVMScore":4.479,"Precursor.Area":1625929.125,"Apex.RT..min.":8.31,"ptmRS..Binomial.Peptide.Score":429.44,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(10): 1206.48; b2+-H(3) O(4) P(9): 539.22; b2+-H(3) O(4) P(10): 603.74; b2+-H(3) O(4) P(17): 1008.91; b2+-H(3) O(4) P(18): 1058.44; b2+-H(3) O(4) P(20): 1163.51; b1+(7): 845.35; b2+(7): 423.18","ptmRS..Best.Site.Probabilities":"S9(Phospho): 99.99","ptmRS..Phospho.Site.Probabilities":"S(5): 0.0; S(7): 0.0; Y(8): 0.0; S(9): 100.0; T(13): 0.0; T(16): 0.0; S(21): 0.0; S(22): 0.0; T(23): 0.0; T(26): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (A4)","Search.ID":"A","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"VFDNIQR","Annotated.Sequence":"[R].VFDNIQR.[R]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.3622,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":446.23776,"Contaminant":"False","Aligned.m.z..Da.":446.23783,"MH...Da.":891.46824,"Theo..MH...Da.":891.46829,"DeltaM..ppm.":-0.06,"Deltam.z..Da.":-0.00003,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2552462.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":62.04779,"Ion.Inject.Time..ms.":22,"RT..min.":8.3034,"First.Scan":6311,"Last.Scan":6311,"Master.Scan.s.":6299,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_03.raw","File.ID":"F36","Quan.Info":"","Peptides.Matched":426,"XCorr":1.85,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":0.00012,"Percolator.SVMScore":1.549,"Precursor.Area":1591560.875,"Apex.RT..min.":8.3,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (A4)","Search.ID":"A","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"QLSEDSHHLQSNNDNFSK","Annotated.Sequence":"[K].QLSEDSHHLQSNNDNFSK.[K]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":4,"Original.Precursor.Charge":4,"DeltaScore":0.48,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":525.73924,"Contaminant":"False","Aligned.m.z..Da.":525.73927,"MH...Da.":2099.93515,"Theo..MH...Da.":2099.9377,"DeltaM..ppm.":-1.22,"Deltam.z..Da.":-0.00064,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1298862,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":6.6447,"First.Scan":4139,"Last.Scan":4139,"Master.Scan.s.":4128,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_03.raw","File.ID":"F36","Quan.Info":"","Peptides.Matched":2142,"XCorr":2.25,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":0.00046,"Percolator.SVMScore":1.267,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (A4)","Search.ID":"A","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ELDEIAASLENL","Annotated.Sequence":"[K].ELDEIAAsLENL.[-]","Modifications":"S8(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7207,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":698.81544,"Contaminant":"False","Aligned.m.z..Da.":698.81577,"MH...Da.":1396.62361,"Theo..MH...Da.":1396.62433,"DeltaM..ppm.":-0.52,"Deltam.z..Da.":-0.00036,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3506202.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":18.2342,"First.Scan":18951,"Last.Scan":18951,"Master.Scan.s.":18948,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_03.raw","File.ID":"F36","Quan.Info":"","Peptides.Matched":950,"XCorr":2.9,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":0.00095,"Percolator.SVMScore":1.113,"Precursor.Area":2605097,"Apex.RT..min.":18.24,"ptmRS..Binomial.Peptide.Score":208.35,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"S8(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(8): 100.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (A4)","Search.ID":"A","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.4664,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40883,"Contaminant":"False","Aligned.m.z..Da.":967.40928,"MH...Da.":2900.21194,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":4.35,"Deltam.z..Da.":0.0042,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":160420.67188,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":9.55191,"Ion.Inject.Time..ms.":22,"RT..min.":18.7956,"First.Scan":19674,"Last.Scan":19674,"Master.Scan.s.":19663,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_03.raw","File.ID":"F36","Quan.Info":"","Peptides.Matched":2686,"XCorr":2.98,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":6.273e-08,"Percolator.SVMScore":3.086,"ptmRS..Binomial.Peptide.Score":325.44,"ptmRS..Isoform.Confidence.Probability":0.5,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"Y12(Phospho): 50; S13(Phospho): 50","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 50.0; S(13): 50.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (A4)","Search.ID":"A","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"IQDPQMTGYVSTR","Annotated.Sequence":"[R].IQDPQMtGYVsTR.[Y]","Modifications":"T7(Phospho); S11(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P32485","Master.Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","Protein.Accessions":"P32485","Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.0471,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":828.33008,"Contaminant":"False","Aligned.m.z..Da.":828.33007,"MH...Da.":1655.65289,"Theo..MH...Da.":1655.65362,"DeltaM..ppm.":-0.44,"Deltam.z..Da.":-0.00036,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2294504,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":33.23195,"Ion.Inject.Time..ms.":22,"RT..min.":9.8289,"First.Scan":8180,"Last.Scan":8180,"Master.Scan.s.":8171,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_03.raw","File.ID":"F36","Quan.Info":"","Peptides.Matched":761,"XCorr":2.76,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":0.00001,"Percolator.SVMScore":2.043,"Precursor.Area":5754031.5,"Apex.RT..min.":9.85,"ptmRS..Binomial.Peptide.Score":356.04,"ptmRS..Isoform.Confidence.Probability":0.005,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(11): 1316.53; y1+-H(3) O(4) P(10): 1201.51; y1+-H(3) O(4) P(8): 976.40; y1+-H(3) O(4) P(7): 845.36; y2+-H(3) O(4) P(11): 658.77; y2+-H(3) O(4) P(10): 601.26; y1+(4): 462.27; y1+(3): 363.20","ptmRS..Best.Site.Probabilities":"T7(Phospho): 100; Y9(Phospho): 99.06","ptmRS..Phospho.Site.Probabilities":"T(7): 100.0; Y(9): 99.1; S(11): 0.5; T(12): 0.5"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (A4)","Search.ID":"A","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MGSINNNFNTNNNSNTDLDR","Annotated.Sequence":"[-].mGSINNNFNTNNNsNTDLDR.[D]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S14(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P39517","Master.Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","Protein.Accessions":"P39517","Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0706,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":749.30805,"Contaminant":"False","Aligned.m.z..Da.":749.30811,"MH...Da.":2245.9096,"Theo..MH...Da.":2245.91057,"DeltaM..ppm.":-0.43,"Deltam.z..Da.":-0.00032,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1157535.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":34.62262,"Ion.Inject.Time..ms.":22,"RT..min.":11.9426,"First.Scan":10897,"Last.Scan":10897,"Master.Scan.s.":10888,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_03.raw","File.ID":"F36","Quan.Info":"","Peptides.Matched":1504,"XCorr":2.69,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":7.622e-06,"Percolator.SVMScore":2.108,"ptmRS..Binomial.Peptide.Score":302.13,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(12): 1359.59; y1+-H(3) O(4) P(11): 1245.55; y1+-H(3) O(4) P(10): 1144.50; y1+-H(3) O(4) P(9): 1030.45; y1+-H(3) O(4) P(8): 916.41; y1+-H(3) O(4) P(7): 802.37; y2+-H(3) O(4) P(8): 458.71; y2+-H(3) O(4) P(7): 401.69; y1+(6): 733.35; y1+(5): 619.30","ptmRS..Best.Site.Probabilities":"S14(Phospho): 99.99","ptmRS..Phospho.Site.Probabilities":"S(3): 0.0; T(10): 0.0; S(14): 100.0; T(16): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (A4)","Search.ID":"A","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MGSINNNFNTNNNSNTDLDR","Annotated.Sequence":"[-].mGSINNNFNTNNNsNTDLDR.[D]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S14(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P39517","Master.Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","Protein.Accessions":"P39517","Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.0926,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1123.46301,"Contaminant":"False","Aligned.m.z..Da.":1123.46311,"MH...Da.":2245.91874,"Theo..MH...Da.":2245.91057,"DeltaM..ppm.":3.64,"Deltam.z..Da.":0.00409,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":855343.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":11.9681,"First.Scan":10929,"Last.Scan":10929,"Master.Scan.s.":10927,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_03.raw","File.ID":"F36","Quan.Info":"","Peptides.Matched":1489,"XCorr":4.75,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":6.746e-12,"Percolator.SVMScore":4.949,"Precursor.Area":840542.0625,"Apex.RT..min.":11.96,"ptmRS..Binomial.Peptide.Score":474.13,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(13): 1506.66; y1+-H(3) O(4) P(12): 1359.59; y1+-H(3) O(4) P(11): 1245.55; y1+-H(3) O(4) P(10): 1144.50; y1+-H(3) O(4) P(9): 1030.45; y1+-H(3) O(4) P(8): 916.41; y1+-H(3) O(4) P(7): 802.37; y1+(6): 733.35; y1+(5): 619.30","ptmRS..Best.Site.Probabilities":"S14(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(3): 0.0; T(10): 0.0; S(14): 100.0; T(16): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (A4)","Search.ID":"A","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"MGSINNNFNTNNNSNTDLDRDWK","Annotated.Sequence":"[-].mGSINNNFNTNNnsNTDLDRDWK.[T]","Modifications":"N-Term(Prot)(Met-loss); N13(Deamidated); S14(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P39517","Master.Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","Protein.Accessions":"P39517","Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0174,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":878.70001,"Contaminant":"False","Aligned.m.z..Da.":878.70007,"MH...Da.":2634.08549,"Theo..MH...Da.":2634.08524,"DeltaM..ppm.":0.09,"Deltam.z..Da.":0.00008,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1177127.375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":7.79802,"Ion.Inject.Time..ms.":22,"RT..min.":11.3998,"First.Scan":10187,"Last.Scan":10187,"Master.Scan.s.":10186,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_03.raw","File.ID":"F36","Quan.Info":"","Peptides.Matched":1950,"XCorr":3.44,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":6.146e-09,"Percolator.SVMScore":3.56,"Precursor.Area":2432182.25,"Apex.RT..min.":11.61,"ptmRS..Binomial.Peptide.Score":373.3,"ptmRS..Isoform.Confidence.Probability":0.991,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(11): 1346.60; y2+-H(3) O(4) P(18): 1082.97; y2+-H(3) O(4) P(17): 1025.95; y2+-H(3) O(4) P(16): 968.92; y2+-H(3) O(4) P(15): 895.39; y2+-H(3) O(4) P(14): 838.37; y2+-H(3) O(4) P(12): 730.82; y2+-H(3) O(4) P(10): 616.29; y1+(9): 1162.55","ptmRS..Best.Site.Probabilities":"S14(Phospho): 99.11","ptmRS..Phospho.Site.Probabilities":"S(3): 0.0; T(10): 0.0; S(14): 99.1; T(16): 0.9"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (A4)","Search.ID":"A","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"SSQIHIENESTEDILK","Annotated.Sequence":"[R].SSQIHIENEStEDILK.[I]","Modifications":"T11(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0355,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":641.6293,"Contaminant":"False","Aligned.m.z..Da.":641.62934,"MH...Da.":1922.87335,"Theo..MH...Da.":1922.87429,"DeltaM..ppm.":-0.49,"Deltam.z..Da.":-0.00031,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2248154,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":11.3226,"First.Scan":10087,"Last.Scan":10087,"Master.Scan.s.":10082,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_03.raw","File.ID":"F36","Quan.Info":"","Peptides.Matched":1803,"XCorr":4.79,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":1.342e-07,"Percolator.SVMScore":2.931,"Precursor.Area":1991789.75,"Apex.RT..min.":11.33,"ptmRS..Binomial.Peptide.Score":319.1,"ptmRS..Isoform.Confidence.Probability":0.5,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(7): 787.42; b2+-H(3) O(4) P(10): 554.26; y2+-H(3) O(4) P(7): 394.21","ptmRS..Best.Site.Probabilities":"S10(Phospho): 50; T11(Phospho): 50","ptmRS..Phospho.Site.Probabilities":"S(1): 0.0; S(2): 0.0; S(10): 50.0; T(11): 50.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (A4)","Search.ID":"A","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KSDEPSRESTPVR","Annotated.Sequence":"[K].KSDEPsREsTPVR.[S]","Modifications":"S6(Phospho); S9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q08421","Master.Protein.Descriptions":"Enhancer of translation termination 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ETT1 PE=1 SV=1","Protein.Accessions":"Q08421","Protein.Descriptions":"Enhancer of translation termination 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ETT1 PE=1 SV=1","X..Missed.Cleavages":2,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.129,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":824.34211,"Contaminant":"False","Aligned.m.z..Da.":824.34215,"MH...Da.":1647.67694,"Theo..MH...Da.":1647.67752,"DeltaM..ppm.":-0.35,"Deltam.z..Da.":-0.00029,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":11321516,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":2.53017,"Ion.Inject.Time..ms.":14.403,"RT..min.":4.5537,"First.Scan":1492,"Last.Scan":1492,"Master.Scan.s.":1489,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_03.raw","File.ID":"F36","Quan.Info":"","Peptides.Matched":970,"XCorr":3.1,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":0.00005,"Percolator.SVMScore":1.735,"Precursor.Area":13340450,"Apex.RT..min.":4.56,"ptmRS..Binomial.Peptide.Score":390.81,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(8): 911.42; b1+-H(3) O(4) P(9): 998.45; b1+-H(3) O(4) P(10): 1179.47; b1+-H(3) O(4) P(12): 1375.59; y1+- -2H(3) O(4) P -2H(3) O(4) P(10): 1121.57; y1+- -2H(3) O(4) P -2H(3) O(4) P(9): 992.53; y2+- -2H(3) O(4) P -2H(3) O(4) P(10): 561.29; y2+- -2H(3) O(4) P -2H(3) O(4) P(9): 496.77; b1+(8): 1009.40; y1+(7): 924.43","ptmRS..Best.Site.Probabilities":"S6(Phospho): 99.55; T10(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(6): 99.6; S(9): 0.5; T(10): 100.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (A4)","Search.ID":"A","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].mSEGITDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7106,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1056.48375,"Contaminant":"False","Aligned.m.z..Da.":1056.484,"MH...Da.":2111.96022,"Theo..MH...Da.":2111.96151,"DeltaM..ppm.":-0.61,"Deltam.z..Da.":-0.00065,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":964942.1875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":14.6129,"First.Scan":14351,"Last.Scan":14351,"Master.Scan.s.":14346,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_03.raw","File.ID":"F36","Quan.Info":"","Peptides.Matched":2300,"XCorr":4.32,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":8.664e-13,"Percolator.SVMScore":5.367,"Precursor.Area":1073634.25,"Apex.RT..min.":14.63,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (A4)","Search.ID":"A","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"AAEAGETGAATSATEGDNNNNTAAGDKK","Annotated.Sequence":"[K].AAEAGETGAATSATEGDNNNNTAAGDKK.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.7556,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":879.39371,"Contaminant":"False","Aligned.m.z..Da.":879.39379,"MH...Da.":2636.16659,"Theo..MH...Da.":2636.16665,"DeltaM..ppm.":-0.02,"Deltam.z..Da.":-0.00002,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":6468078,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":3.23171,"Ion.Inject.Time..ms.":22,"RT..min.":4.9025,"First.Scan":1935,"Last.Scan":1935,"Master.Scan.s.":1931,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_03.raw","File.ID":"F36","Quan.Info":"","Peptides.Matched":3575,"XCorr":8.51,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":6.305e-16,"Percolator.SVMScore":10.005,"Precursor.Area":4066932,"Apex.RT..min.":4.9,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (A4)","Search.ID":"A","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"AAEAGETGAATSATEGDNNNNTAAGDKK","Annotated.Sequence":"[K].AAEAGETGAATsATEGDNNNNTAAGDKK.[G]","Modifications":"S12(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0174,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":906.04914,"Contaminant":"False","Aligned.m.z..Da.":906.04922,"MH...Da.":2716.13286,"Theo..MH...Da.":2716.13298,"DeltaM..ppm.":-0.05,"Deltam.z..Da.":-0.00004,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":6423006.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":4.9228,"First.Scan":1960,"Last.Scan":1960,"Master.Scan.s.":1957,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_03.raw","File.ID":"F36","Quan.Info":"","Peptides.Matched":2565,"XCorr":7.49,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":6.305e-16,"Percolator.SVMScore":7.235,"Precursor.Area":10149140,"Apex.RT..min.":4.93,"ptmRS..Binomial.Peptide.Score":504.79,"ptmRS..Isoform.Confidence.Probability":0.992,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(15): 1300.57; y1+-H(3) O(4) P(18): 1789.79; y2+-H(3) O(4) P(21): 994.95; y2+-H(3) O(4) P(18): 895.40; y2+-H(3) O(4) P(17): 844.88","ptmRS..Best.Site.Probabilities":"S12(Phospho): 99.24","ptmRS..Phospho.Site.Probabilities":"T(7): 0.0; T(11): 0.8; S(12): 99.2; T(14): 0.0; T(22): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (A4)","Search.ID":"A","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].msEGITDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S2(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.0925,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1096.47021,"Contaminant":"False","Aligned.m.z..Da.":1096.47007,"MH...Da.":2191.93315,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":2.42,"Deltam.z..Da.":0.00265,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":577502.375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":13.80654,"Ion.Inject.Time..ms.":22,"RT..min.":15.9659,"First.Scan":16051,"Last.Scan":16051,"Master.Scan.s.":16049,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_03.raw","File.ID":"F36","Quan.Info":"","Peptides.Matched":2159,"XCorr":4.54,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":2.932e-11,"Percolator.SVMScore":4.65,"Precursor.Area":51609864,"Apex.RT..min.":15.73,"ptmRS..Binomial.Peptide.Score":500.81,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(2): 112.04; b1+-H(3) O(4) P(3): 241.08; b1+-H(3) O(4) P(4): 298.10; b1+-H(3) O(4) P(5): 411.19; b1+-H(3) O(4) P(6): 512.24; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+-H(3) O(4) P(9): 869.39; b1+-H(3) O(4) P(10): 998.43; b1+(5): 509.16; y1+(14): 1683.77","ptmRS..Best.Site.Probabilities":"S2(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 100.0; T(6): 0.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (A4)","Search.ID":"A","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FDDMELDENLLR","Annotated.Sequence":"[K].FDDMELDENLLR.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6274,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":755.34789,"Contaminant":"False","Aligned.m.z..Da.":755.34779,"MH...Da.":1509.68851,"Theo..MH...Da.":1509.68898,"DeltaM..ppm.":-0.32,"Deltam.z..Da.":-0.00024,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":429816.375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":15.20364,"Ion.Inject.Time..ms.":22,"RT..min.":16.0531,"First.Scan":16164,"Last.Scan":16164,"Master.Scan.s.":16153,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_03.raw","File.ID":"F36","Quan.Info":"","Peptides.Matched":1068,"XCorr":3.14,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":1.016e-08,"Percolator.SVMScore":3.457,"Precursor.Area":319446.75,"Apex.RT..min.":16.05,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (A4)","Search.ID":"A","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":4,"DeltaScore":0.0085,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40792,"Contaminant":"False","Aligned.m.z..Da.":967.40781,"MH...Da.":2900.20922,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":3.41,"Deltam.z..Da.":0.0033,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1912810.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":30.99374,"Ion.Inject.Time..ms.":22,"RT..min.":15.7538,"First.Scan":15781,"Last.Scan":15781,"Master.Scan.s.":15776,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_03.raw","File.ID":"F36","Quan.Info":"","Peptides.Matched":2609,"XCorr":4.72,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":1.273e-07,"Percolator.SVMScore":2.942,"ptmRS..Binomial.Peptide.Score":307.83,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(13): 1413.58; y2+-H(3) O(4) P(14): 811.37; b1+(12): 1344.56","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (A4)","Search.ID":"A","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].msEGITDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S2(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":3,"DeltaScore":0.1429,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1096.46821,"Contaminant":"False","Aligned.m.z..Da.":1096.4681,"MH...Da.":2191.92914,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":0.59,"Deltam.z..Da.":0.00065,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":6893081,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":27.15997,"Ion.Inject.Time..ms.":22,"RT..min.":15.6947,"First.Scan":15707,"Last.Scan":15707,"Master.Scan.s.":15698,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_03.raw","File.ID":"F36","Quan.Info":"","Peptides.Matched":1989,"XCorr":4.97,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":1.504e-10,"Percolator.SVMScore":4.316,"Precursor.Area":51609864,"Apex.RT..min.":15.73,"ptmRS..Binomial.Peptide.Score":595.45,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(3): 241.08; b1+-H(3) O(4) P(4): 298.10; b1+-H(3) O(4) P(5): 411.19; b1+-H(3) O(4) P(6): 512.24; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+-H(3) O(4) P(9): 869.39; b1+-H(3) O(4) P(10): 998.43; b1+-H(3) O(4) P(11): 1085.46; b1+-H(3) O(4) P(12): 1213.52; b1+-H(3) O(4) P(13): 1326.61; b1+(4): 396.08; b1+(5): 509.16; y1+(14): 1683.77","ptmRS..Best.Site.Probabilities":"S2(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 100.0; T(6): 0.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (A4)","Search.ID":"A","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0225,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.4039,"Contaminant":"False","Aligned.m.z..Da.":967.40379,"MH...Da.":2900.19716,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":-0.75,"Deltam.z..Da.":-0.00072,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1619966.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":39.29428,"Ion.Inject.Time..ms.":22,"RT..min.":15.8025,"First.Scan":15843,"Last.Scan":15843,"Master.Scan.s.":15841,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_03.raw","File.ID":"F36","Quan.Info":"","Peptides.Matched":2382,"XCorr":4.44,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":1.386e-08,"Percolator.SVMScore":3.394,"Precursor.Area":2813725.75,"Apex.RT..min.":15.77,"ptmRS..Binomial.Peptide.Score":369.89,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(13): 1413.58; y2+-H(3) O(4) P(14): 811.37; b1+(12): 1344.56","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (A4)","Search.ID":"A","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MFILDEADEMLSSGFK","Annotated.Sequence":"[K].mFILDEADEmLSSGFK.[E]","Modifications":"M1(Oxidation); M10(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6881,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":932.91837,"Contaminant":"False","Aligned.m.z..Da.":932.91836,"MH...Da.":1864.82946,"Theo..MH...Da.":1864.83433,"DeltaM..ppm.":-2.61,"Deltam.z..Da.":-0.00244,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":314077.125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":6.77365,"Ion.Inject.Time..ms.":22,"RT..min.":16.7052,"First.Scan":16993,"Last.Scan":16993,"Master.Scan.s.":16985,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_03.raw","File.ID":"F36","Quan.Info":"","Peptides.Matched":1828,"XCorr":2.95,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":2.92e-09,"Percolator.SVMScore":3.712,"Precursor.Area":656297.9375,"Apex.RT..min.":16.73,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (A4)","Search.ID":"A","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":5,"DeltaScore":0.0565,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40692,"Contaminant":"False","Aligned.m.z..Da.":967.40693,"MH...Da.":2900.20621,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":2.37,"Deltam.z..Da.":0.0023,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":268829.6875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":49.08233,"Ion.Inject.Time..ms.":22,"RT..min.":16.723,"First.Scan":17015,"Last.Scan":17015,"Master.Scan.s.":17011,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_03.raw","File.ID":"F36","Quan.Info":"","Peptides.Matched":2619,"XCorr":4.25,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":8.697e-11,"Percolator.SVMScore":4.428,"ptmRS..Binomial.Peptide.Score":446.6,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(14): 1621.73; b2+-H(3) O(4) P(16): 886.35; b2+-H(3) O(4) P(18): 1014.90; y2+-H(3) O(4) P(14): 811.37; b1+(12): 1344.56","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (A4)","Search.ID":"A","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MFILDEADEMLSSGFK","Annotated.Sequence":"[K].mFILDEADEmLSSGFK.[E]","Modifications":"M1(Oxidation); M10(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.6324,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":622.28193,"Contaminant":"False","Aligned.m.z..Da.":622.28193,"MH...Da.":1864.83124,"Theo..MH...Da.":1864.83433,"DeltaM..ppm.":-1.66,"Deltam.z..Da.":-0.00103,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":475856.5625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":35.77242,"Ion.Inject.Time..ms.":22,"RT..min.":16.728,"First.Scan":17023,"Last.Scan":17023,"Master.Scan.s.":17011,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_03.raw","File.ID":"F36","Quan.Info":"","Peptides.Matched":1923,"XCorr":2.53,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":9.97e-06,"Percolator.SVMScore":2.053,"Precursor.Area":472991.0625,"Apex.RT..min.":16.81,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (A4)","Search.ID":"A","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEySDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); Y12(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":5,"DeltaScore":0.3962,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40918,"Contaminant":"False","Aligned.m.z..Da.":967.40907,"MH...Da.":2900.21299,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":4.71,"Deltam.z..Da.":0.00455,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":779899.375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":17.75669,"Ion.Inject.Time..ms.":22,"RT..min.":16.2109,"First.Scan":16362,"Last.Scan":16362,"Master.Scan.s.":16361,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_03.raw","File.ID":"F36","Quan.Info":"","Peptides.Matched":2674,"XCorr":4.19,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":1.574e-08,"Percolator.SVMScore":3.368,"ptmRS..Binomial.Peptide.Score":312.09,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"b2+-H(3) O(4) P(18): 1014.90; y2+-H(3) O(4) P(14): 811.37; b1+(12): 1344.56","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (A4)","Search.ID":"A","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0132,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40584,"Contaminant":"False","Aligned.m.z..Da.":967.40573,"MH...Da.":2900.20296,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":1.25,"Deltam.z..Da.":0.00121,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":823280.875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":40.66093,"Ion.Inject.Time..ms.":22,"RT..min.":16.2561,"First.Scan":16421,"Last.Scan":16421,"Master.Scan.s.":16413,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_03.raw","File.ID":"F36","Quan.Info":"","Peptides.Matched":2563,"XCorr":3.8,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":6.792e-09,"Percolator.SVMScore":3.539,"Precursor.Area":820895,"Apex.RT..min.":16.24,"ptmRS..Binomial.Peptide.Score":336.81,"ptmRS..Isoform.Confidence.Probability":0.992,"ptmRS..Isoform.Group.Report":"b1+(12): 1344.56","ptmRS..Best.Site.Probabilities":"S13(Phospho): 99.2","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.8; S(13): 99.2; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (A4)","Search.ID":"A","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":5,"DeltaScore":0.0664,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40346,"Contaminant":"False","Aligned.m.z..Da.":967.40336,"MH...Da.":2900.19582,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":-1.21,"Deltam.z..Da.":-0.00117,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1454138.375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":27.79925,"Ion.Inject.Time..ms.":22,"RT..min.":15.669,"First.Scan":15673,"Last.Scan":15673,"Master.Scan.s.":15672,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_03.raw","File.ID":"F36","Quan.Info":"","Peptides.Matched":2281,"XCorr":5.87,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":1.309e-11,"Percolator.SVMScore":4.814,"Precursor.Area":2813725.75,"Apex.RT..min.":15.77,"ptmRS..Binomial.Peptide.Score":562.95,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(13): 1413.58; b1+-H(3) O(4) P(14): 1528.61; b1+-H(3) O(4) P(16): 1771.69; y1+-H(3) O(4) P(15): 1750.78; y1+-H(3) O(4) P(14): 1621.73; y1+-H(3) O(4) P(13): 1458.67; b2+-H(3) O(4) P(18): 1014.90; y2+-H(3) O(4) P(14): 811.37; b1+(12): 1344.56","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (A4)","Search.ID":"A","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FTVSAIYSDLPQQER","Annotated.Sequence":"[K].FTVSAIYSDLPQQER.[D]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6529,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":877.43887,"Contaminant":"False","Aligned.m.z..Da.":877.43894,"MH...Da.":1753.87046,"Theo..MH...Da.":1753.87554,"DeltaM..ppm.":-2.9,"Deltam.z..Da.":-0.00254,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":951984.3125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":15.0443,"First.Scan":14890,"Last.Scan":14890,"Master.Scan.s.":14879,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_03.raw","File.ID":"F36","Quan.Info":"","Peptides.Matched":1255,"XCorr":3.4,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":1.051e-09,"Percolator.SVMScore":3.92,"Precursor.Area":530850.8125,"Apex.RT..min.":15.04,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (A4)","Search.ID":"A","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"GVFGYGFEEPSAIQQR","Annotated.Sequence":"[R].GVFGYGFEEPSAIQQR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6927,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":892.93473,"Contaminant":"False","Aligned.m.z..Da.":892.9348,"MH...Da.":1784.86218,"Theo..MH...Da.":1784.86022,"DeltaM..ppm.":1.09,"Deltam.z..Da.":0.00098,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":609622.3125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":15.0527,"First.Scan":14900,"Last.Scan":14900,"Master.Scan.s.":14892,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_03.raw","File.ID":"F36","Quan.Info":"","Peptides.Matched":1456,"XCorr":4.23,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":5.372e-12,"Percolator.SVMScore":4.995,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (A4)","Search.ID":"A","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":5,"DeltaScore":0.0731,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40506,"Contaminant":"False","Aligned.m.z..Da.":967.4051,"MH...Da.":2900.20062,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":0.45,"Deltam.z..Da.":0.00043,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":10447828,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":15.29885,"Ion.Inject.Time..ms.":16.31,"RT..min.":15.1537,"First.Scan":15026,"Last.Scan":15026,"Master.Scan.s.":15022,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_03.raw","File.ID":"F36","Quan.Info":"","Peptides.Matched":2487,"XCorr":7.52,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":2.651e-15,"Percolator.SVMScore":6.548,"Precursor.Area":118135560,"Apex.RT..min.":15.06,"ptmRS..Binomial.Peptide.Score":690.03,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(13): 1413.58; b1+-H(3) O(4) P(14): 1528.61; b1+-H(3) O(4) P(16): 1771.69; y1+-H(3) O(4) P(15): 1750.78; y1+-H(3) O(4) P(14): 1621.73; y1+-H(3) O(4) P(13): 1458.67; b2+-H(3) O(4) P(16): 886.35; b2+-H(3) O(4) P(18): 1014.90; y2+-H(3) O(4) P(14): 811.37; b1+(12): 1344.56; y1+(13): 1556.65","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (A4)","Search.ID":"A","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].mSEGITDIEEsQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S11(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1897,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1096.46862,"Contaminant":"False","Aligned.m.z..Da.":1096.46875,"MH...Da.":2191.92996,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":0.97,"Deltam.z..Da.":0.00106,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":316281.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":14.9405,"First.Scan":14760,"Last.Scan":14760,"Master.Scan.s.":14749,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_03.raw","File.ID":"F36","Quan.Info":"","Peptides.Matched":2040,"XCorr":4.27,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":3.95e-08,"Percolator.SVMScore":3.181,"Precursor.Area":642230.5,"Apex.RT..min.":14.95,"ptmRS..Binomial.Peptide.Score":425.96,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(14): 1665.76; y1+-H(3) O(4) P(13): 1564.71; y1+-H(3) O(4) P(12): 1449.69; y1+-H(3) O(4) P(11): 1336.60; y1+-H(3) O(4) P(10): 1207.56; y1+-H(3) O(4) P(9): 1078.52; y2+-H(3) O(4) P(18): 1026.47; y1+(8): 1009.49; y1+(7): 881.44; y1+(6): 768.35; y1+(5): 640.29","ptmRS..Best.Site.Probabilities":"S11(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; T(6): 0.0; S(11): 100.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (A4)","Search.ID":"A","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].mSEGITDIEEsQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S11(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0179,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":731.31321,"Contaminant":"False","Aligned.m.z..Da.":731.31329,"MH...Da.":2191.92507,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":-1.26,"Deltam.z..Da.":-0.00092,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":505013.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":13.48413,"Ion.Inject.Time..ms.":22,"RT..min.":14.9601,"First.Scan":14784,"Last.Scan":14784,"Master.Scan.s.":14775,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_03.raw","File.ID":"F36","Quan.Info":"","Peptides.Matched":1903,"XCorr":2.24,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":0.00001,"Percolator.SVMScore":1.97,"Precursor.Area":224384.04688,"Apex.RT..min.":14.95,"ptmRS..Binomial.Peptide.Score":244.45,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(11): 1336.60; b1+(6): 530.25","ptmRS..Best.Site.Probabilities":"S11(Phospho): 99.99","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; T(6): 0.0; S(11): 100.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (A4)","Search.ID":"A","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].mSEGItDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); T6(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.1357,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":731.31428,"Contaminant":"False","Aligned.m.z..Da.":731.31424,"MH...Da.":2191.92829,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":0.2,"Deltam.z..Da.":0.00015,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1330068.125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":15.4747,"First.Scan":15427,"Last.Scan":15427,"Master.Scan.s.":15425,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_03.raw","File.ID":"F36","Quan.Info":"","Peptides.Matched":2019,"XCorr":5.6,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":1.983e-10,"Percolator.SVMScore":4.26,"Precursor.Area":1451382.875,"Apex.RT..min.":15.49,"ptmRS..Binomial.Peptide.Score":423.19,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(6): 512.24; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+-H(3) O(4) P(9): 869.39; b1+-H(3) O(4) P(10): 998.43; b1+(2): 130.05; b1+(3): 259.09; b1+(4): 316.11; b1+(5): 429.20; b2+(3): 130.05","ptmRS..Best.Site.Probabilities":"T6(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; T(6): 100.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (A4)","Search.ID":"A","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0363,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40696,"Contaminant":"False","Aligned.m.z..Da.":967.40696,"MH...Da.":2900.20634,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":2.42,"Deltam.z..Da.":0.00234,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":4519173.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":47.67368,"Ion.Inject.Time..ms.":22,"RT..min.":15.2878,"First.Scan":15192,"Last.Scan":15192,"Master.Scan.s.":15191,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_03.raw","File.ID":"F36","Quan.Info":"","Peptides.Matched":2595,"XCorr":5.79,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":1.033e-09,"Percolator.SVMScore":3.923,"ptmRS..Binomial.Peptide.Score":545.64,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(13): 1413.58; b1+-H(3) O(4) P(14): 1528.61; b1+-H(3) O(4) P(15): 1642.65; b1+-H(3) O(4) P(16): 1771.69; y1+-H(3) O(4) P(15): 1750.78; y1+-H(3) O(4) P(14): 1621.73; y1+-H(3) O(4) P(13): 1458.67; b2+-H(3) O(4) P(18): 1014.90; y2+-H(3) O(4) P(14): 811.37; b1+(12): 1344.56","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (A4)","Search.ID":"A","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].mSEGItDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); T6(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.118,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1096.46562,"Contaminant":"False","Aligned.m.z..Da.":1096.46556,"MH...Da.":2191.92396,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":-1.77,"Deltam.z..Da.":-0.00194,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":420628.15625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":43.21026,"Ion.Inject.Time..ms.":22,"RT..min.":15.4574,"First.Scan":15406,"Last.Scan":15406,"Master.Scan.s.":15399,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_03.raw","File.ID":"F36","Quan.Info":"","Peptides.Matched":1841,"XCorr":5.17,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":9.054e-11,"Percolator.SVMScore":4.42,"Precursor.Area":51609864,"Apex.RT..min.":15.73,"ptmRS..Binomial.Peptide.Score":456.59,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(6): 512.24; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+-H(3) O(4) P(9): 869.39; b1+-H(3) O(4) P(10): 998.43; y1+-H(3) O(4) P(14): 1665.76; b1+(3): 259.09; b1+(4): 316.11; b1+(5): 429.20","ptmRS..Best.Site.Probabilities":"T6(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; T(6): 100.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (A4)","Search.ID":"A","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].mSEGItDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); T6(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.0575,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1096.46562,"Contaminant":"False","Aligned.m.z..Da.":1096.46556,"MH...Da.":2191.92396,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":-1.77,"Deltam.z..Da.":-0.00194,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":420628.15625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":82.93734,"Ion.Inject.Time..ms.":22,"RT..min.":15.4586,"First.Scan":15408,"Last.Scan":15408,"Master.Scan.s.":15399,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_03.raw","File.ID":"F36","Quan.Info":"","Peptides.Matched":1841,"XCorr":3.65,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":4.649e-09,"Percolator.SVMScore":3.617,"Precursor.Area":51609864,"Apex.RT..min.":15.73,"ptmRS..Binomial.Peptide.Score":466.15,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(6): 512.24; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+-H(3) O(4) P(9): 869.39; b1+-H(3) O(4) P(10): 998.43; b1+-H(3) O(4) P(11): 1085.46; b1+-H(3) O(4) P(12): 1213.52; b1+(3): 259.09; b1+(4): 316.11; b1+(5): 429.20","ptmRS..Best.Site.Probabilities":"T6(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; T(6): 100.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (A4)","Search.ID":"A","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEySDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); Y12(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0133,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.4037,"Contaminant":"False","Aligned.m.z..Da.":967.40374,"MH...Da.":2900.19654,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":-0.96,"Deltam.z..Da.":-0.00093,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":583811.3125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":52.74138,"Ion.Inject.Time..ms.":22,"RT..min.":16.8256,"First.Scan":17147,"Last.Scan":17147,"Master.Scan.s.":17141,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_03.raw","File.ID":"F36","Quan.Info":"","Peptides.Matched":2349,"XCorr":3,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":1.544e-08,"Percolator.SVMScore":3.372,"ptmRS..Binomial.Peptide.Score":270.86,"ptmRS..Isoform.Confidence.Probability":0.5,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"Y12(Phospho): 50; S13(Phospho): 50","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 50.0; S(13): 50.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (A4)","Search.ID":"A","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0142,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40231,"Contaminant":"False","Aligned.m.z..Da.":967.40235,"MH...Da.":2900.19238,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":-2.4,"Deltam.z..Da.":-0.00232,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":630038.0625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":29.62127,"Ion.Inject.Time..ms.":22,"RT..min.":16.844,"First.Scan":17170,"Last.Scan":17170,"Master.Scan.s.":17167,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_03.raw","File.ID":"F36","Quan.Info":"","Peptides.Matched":2168,"XCorr":4.24,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":2.233e-08,"Percolator.SVMScore":3.297,"ptmRS..Binomial.Peptide.Score":357.22,"ptmRS..Isoform.Confidence.Probability":0.992,"ptmRS..Isoform.Group.Report":"b2+-H(3) O(4) P(18): 1014.90","ptmRS..Best.Site.Probabilities":"S13(Phospho): 99.24","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.8; S(13): 99.2; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (A4)","Search.ID":"A","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0031,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.4053,"Contaminant":"False","Aligned.m.z..Da.":967.40565,"MH...Da.":2900.20135,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":0.7,"Deltam.z..Da.":0.00067,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":705348.8125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":15.84795,"Ion.Inject.Time..ms.":22,"RT..min.":13.9579,"First.Scan":13528,"Last.Scan":13528,"Master.Scan.s.":13527,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_03.raw","File.ID":"F36","Quan.Info":"","Peptides.Matched":2512,"XCorr":3.18,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":1.191e-06,"Percolator.SVMScore":2.486,"Precursor.Area":552261.4375,"Apex.RT..min.":13.96,"ptmRS..Binomial.Peptide.Score":262.1,"ptmRS..Isoform.Confidence.Probability":0.5,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"Y12(Phospho): 50; S13(Phospho): 50","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 50.0; S(13): 50.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (A4)","Search.ID":"A","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KFQDDTLNR","Annotated.Sequence":"[K].KFQDDtLNR.[V]","Modifications":"T6(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7337,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":608.77112,"Contaminant":"False","Aligned.m.z..Da.":608.77116,"MH...Da.":1216.53496,"Theo..MH...Da.":1216.53579,"DeltaM..ppm.":-0.69,"Deltam.z..Da.":-0.00042,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3723650.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":10.85994,"Ion.Inject.Time..ms.":22,"RT..min.":6.2666,"First.Scan":3638,"Last.Scan":3638,"Master.Scan.s.":3634,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_03.raw","File.ID":"F36","Quan.Info":"","Peptides.Matched":607,"XCorr":3.38,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":0.00001,"Percolator.SVMScore":1.987,"Precursor.Area":4916439,"Apex.RT..min.":6.28,"ptmRS..Binomial.Peptide.Score":373.06,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"T6(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"T(6): 100.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (A4)","Search.ID":"A","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"SQMSVELDDDADLDDELAQLK","Annotated.Sequence":"[R].SQmsVELDDDADLDDELAQLK.[G]","Modifications":"M3(Oxidation); S4(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q08421","Master.Protein.Descriptions":"Enhancer of translation termination 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ETT1 PE=1 SV=1","Protein.Accessions":"Q08421","Protein.Descriptions":"Enhancer of translation termination 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ETT1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.1117,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":816.01212,"Contaminant":"False","Aligned.m.z..Da.":816.01235,"MH...Da.":2446.02182,"Theo..MH...Da.":2446.02149,"DeltaM..ppm.":0.13,"Deltam.z..Da.":0.00011,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":484702.9375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":18.19749,"Ion.Inject.Time..ms.":22,"RT..min.":17.4563,"First.Scan":17955,"Last.Scan":17955,"Master.Scan.s.":17947,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_03.raw","File.ID":"F36","Quan.Info":"","Peptides.Matched":2040,"XCorr":3.85,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":1.041e-06,"Percolator.SVMScore":2.514,"ptmRS..Binomial.Peptide.Score":352.76,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(4): 432.15; b1+-H(3) O(4) P(5): 531.22; b1+-H(3) O(4) P(6): 660.27; b1+-H(3) O(4) P(7): 773.35; b1+-H(3) O(4) P(8): 888.38; b1+(2): 216.10; b1+(3): 363.13","ptmRS..Best.Site.Probabilities":"S4(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(1): 0.0; S(4): 100.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (A4)","Search.ID":"A","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DIFSNDELLSDAYDAK","Annotated.Sequence":"[K].DIFSNDELLSDAYDAK.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7412,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":908.42043,"Contaminant":"False","Aligned.m.z..Da.":908.42055,"MH...Da.":1815.83358,"Theo..MH...Da.":1815.82831,"DeltaM..ppm.":2.9,"Deltam.z..Da.":0.00263,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":435191.65625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":17.0796,"First.Scan":17473,"Last.Scan":17473,"Master.Scan.s.":17466,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_03.raw","File.ID":"F36","Quan.Info":"","Peptides.Matched":1743,"XCorr":3.98,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":1.526e-09,"Percolator.SVMScore":3.844,"Precursor.Area":1982517.875,"Apex.RT..min.":17.1,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (A4)","Search.ID":"A","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DIFSNDELLSDAYDAK","Annotated.Sequence":"[K].DIFSNDELLSDAYDAK.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.697,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":605.94866,"Contaminant":"False","Aligned.m.z..Da.":605.94875,"MH...Da.":1815.83144,"Theo..MH...Da.":1815.82831,"DeltaM..ppm.":1.72,"Deltam.z..Da.":0.00104,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":288262,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":27.58921,"Ion.Inject.Time..ms.":22,"RT..min.":17.0903,"First.Scan":17487,"Last.Scan":17487,"Master.Scan.s.":17479,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_03.raw","File.ID":"F36","Quan.Info":"","Peptides.Matched":1696,"XCorr":2.64,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":0.00003,"Percolator.SVMScore":1.805,"Precursor.Area":495538.03125,"Apex.RT..min.":17.1,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (A4)","Search.ID":"A","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":5,"DeltaScore":0.0132,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40613,"Contaminant":"False","Aligned.m.z..Da.":967.40625,"MH...Da.":2900.20383,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":1.55,"Deltam.z..Da.":0.0015,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":493906.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":48.75518,"Ion.Inject.Time..ms.":22,"RT..min.":17.0408,"First.Scan":17424,"Last.Scan":17424,"Master.Scan.s.":17414,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_03.raw","File.ID":"F36","Quan.Info":"","Peptides.Matched":2579,"XCorr":3.78,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":8.368e-09,"Percolator.SVMScore":3.497,"ptmRS..Binomial.Peptide.Score":400.55,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b2+-H(3) O(4) P(18): 1014.90; y2+-H(3) O(4) P(14): 811.37","ptmRS..Best.Site.Probabilities":"S13(Phospho): 99.99","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (A4)","Search.ID":"A","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DIFSNDELLSDAYDAK","Annotated.Sequence":"[K].DIFSNDELLSDAYDAK.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":2,"DeltaScore":0.6192,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":605.94812,"Contaminant":"False","Aligned.m.z..Da.":605.94821,"MH...Da.":1815.82981,"Theo..MH...Da.":1815.82831,"DeltaM..ppm.":0.82,"Deltam.z..Da.":0.0005,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":683561.0625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":35.21414,"Ion.Inject.Time..ms.":22,"RT..min.":17.0991,"First.Scan":17498,"Last.Scan":17498,"Master.Scan.s.":17492,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_03.raw","File.ID":"F36","Quan.Info":"","Peptides.Matched":1573,"XCorr":2.39,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":0.00003,"Percolator.SVMScore":1.833,"Precursor.Area":495538.03125,"Apex.RT..min.":17.1,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (A4)","Search.ID":"A","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FFISNPDLVDR","Annotated.Sequence":"[R].FFIsNPDLVDR.[L]","Modifications":"S4(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q03558","Master.Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","Protein.Accessions":"Q03558","Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7526,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":701.82278,"Contaminant":"False","Aligned.m.z..Da.":701.82285,"MH...Da.":1402.63829,"Theo..MH...Da.":1402.64026,"DeltaM..ppm.":-1.4,"Deltam.z..Da.":-0.00098,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":593441.0625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":22.34398,"Ion.Inject.Time..ms.":22,"RT..min.":16.9778,"First.Scan":17343,"Last.Scan":17343,"Master.Scan.s.":17336,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_03.raw","File.ID":"F36","Quan.Info":"","Peptides.Matched":859,"XCorr":1.9,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":0.00009,"Percolator.SVMScore":1.604,"Precursor.Area":370934.75,"Apex.RT..min.":16.97,"ptmRS..Binomial.Peptide.Score":280.93,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"S4(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(4): 100.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (A4)","Search.ID":"A","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0885,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":953.40478,"Contaminant":"False","Aligned.m.z..Da.":953.40512,"MH...Da.":2858.19979,"Theo..MH...Da.":2858.18876,"DeltaM..ppm.":3.86,"Deltam.z..Da.":0.00367,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":959586.875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":13.8056,"First.Scan":13335,"Last.Scan":13335,"Master.Scan.s.":13332,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_03.raw","File.ID":"F36","Quan.Info":"","Peptides.Matched":2663,"XCorr":5.65,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":7.374e-10,"Percolator.SVMScore":3.992,"Precursor.Area":3121034.5,"Apex.RT..min.":13.89,"ptmRS..Binomial.Peptide.Score":491.91,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(13): 1371.57; b1+-H(3) O(4) P(14): 1486.60; b1+-H(3) O(4) P(16): 1729.68; y1+-H(3) O(4) P(14): 1621.73; y1+-H(3) O(4) P(13): 1458.67; y2+-H(3) O(4) P(14): 811.37; b1+(12): 1302.55","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (A4)","Search.ID":"A","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"AAEAGETGAATSATEGDNNNNTAAGDK","Annotated.Sequence":"[K].AAEAGETGAATSATEGDNNNNTAAGDK.[K]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.7429,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":836.69507,"Contaminant":"False","Aligned.m.z..Da.":836.69517,"MH...Da.":2508.07066,"Theo..MH...Da.":2508.07169,"DeltaM..ppm.":-0.41,"Deltam.z..Da.":-0.00034,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2963335,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":3.95024,"Ion.Inject.Time..ms.":22,"RT..min.":5.5157,"First.Scan":2690,"Last.Scan":2690,"Master.Scan.s.":2685,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_03.raw","File.ID":"F36","Quan.Info":"","Peptides.Matched":2728,"XCorr":7.35,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":3.383e-14,"Percolator.SVMScore":6.029,"Precursor.Area":2821521.75,"Apex.RT..min.":5.52,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (A4)","Search.ID":"A","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"AAEAGETGAATSATEGDNNNNTAAGDK","Annotated.Sequence":"[K].AAEAGETGAATsATEGDNNNNTAAGDK.[K]","Modifications":"S12(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0184,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":863.35264,"Contaminant":"False","Aligned.m.z..Da.":863.35275,"MH...Da.":2588.04338,"Theo..MH...Da.":2588.03802,"DeltaM..ppm.":2.07,"Deltam.z..Da.":0.00179,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2339530,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":5.5992,"First.Scan":2793,"Last.Scan":2793,"Master.Scan.s.":2789,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_03.raw","File.ID":"F36","Quan.Info":"","Peptides.Matched":1672,"XCorr":5.99,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":2.421e-11,"Percolator.SVMScore":4.689,"Precursor.Area":4794819,"Apex.RT..min.":5.62,"ptmRS..Binomial.Peptide.Score":463.18,"ptmRS..Isoform.Confidence.Probability":0.988,"ptmRS..Isoform.Group.Report":"b2+-H(3) O(4) P(26): 1172.48; y2+-H(3) O(4) P(25): 1174.50; y2+-H(3) O(4) P(21): 981.43; y2+-H(3) O(4) P(16): 780.83","ptmRS..Best.Site.Probabilities":"S12(Phospho): 98.78","ptmRS..Phospho.Site.Probabilities":"T(7): 0.0; T(11): 0.6; S(12): 98.8; T(14): 0.6; T(22): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (W4)","Search.ID":"W","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"APQALMLAPTR","Annotated.Sequence":"[K].APQALMLAPTR.[E]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6375,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":584.82841,"Contaminant":"False","Aligned.m.z..Da.":584.82839,"MH...Da.":1168.64954,"Theo..MH...Da.":1168.65069,"DeltaM..ppm.":-0.98,"Deltam.z..Da.":-0.00057,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":514132.3125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":53.47138,"Ion.Inject.Time..ms.":22,"RT..min.":11.6323,"First.Scan":10415,"Last.Scan":10415,"Master.Scan.s.":10414,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_02.raw","File.ID":"F14","Quan.Info":"","Peptides.Matched":182,"XCorr":2.4,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":0.00001,"Percolator.SVMScore":1.951,"Precursor.Area":455236.21875,"Apex.RT..min.":11.62,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (W4)","Search.ID":"W","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MGSINNNFNTNNNSNTDLDRDWK","Annotated.Sequence":"[-].mGSINNNFNTNNNsNTDLDRDWK.[T]","Modifications":"N-Term(Prot)(Met-loss); S14(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P39517","Master.Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","Protein.Accessions":"P39517","Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.057,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":878.3712,"Contaminant":"False","Aligned.m.z..Da.":878.37118,"MH...Da.":2633.09907,"Theo..MH...Da.":2633.10123,"DeltaM..ppm.":-0.82,"Deltam.z..Da.":-0.00072,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2871606,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":1.86822,"Ion.Inject.Time..ms.":22,"RT..min.":11.2897,"First.Scan":9964,"Last.Scan":9964,"Master.Scan.s.":9959,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_02.raw","File.ID":"F14","Quan.Info":"","Peptides.Matched":2206,"XCorr":6.67,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":7.431e-15,"Percolator.SVMScore":6.159,"Precursor.Area":3088760.75,"Apex.RT..min.":11.3,"ptmRS..Binomial.Peptide.Score":540.37,"ptmRS..Isoform.Confidence.Probability":0.995,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(11): 1345.61; y1+-H(3) O(4) P(10): 1231.57; y2+-H(3) O(4) P(19): 1139.50; y2+-H(3) O(4) P(18): 1082.48; y2+-H(3) O(4) P(17): 1025.45; y2+-H(3) O(4) P(16): 968.43; y2+-H(3) O(4) P(15): 894.90; y2+-H(3) O(4) P(14): 837.88; y2+-H(3) O(4) P(13): 787.35; y2+-H(3) O(4) P(12): 730.33; y2+-H(3) O(4) P(11): 673.31; y2+-H(3) O(4) P(10): 616.29; y1+(9): 1162.55","ptmRS..Best.Site.Probabilities":"S14(Phospho): 99.51","ptmRS..Phospho.Site.Probabilities":"S(3): 0.0; T(10): 0.0; S(14): 99.5; T(16): 0.5"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (W4)","Search.ID":"W","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"HGIVEEK","Annotated.Sequence":"[K].HGIVEEK.[I]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5219,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":406.2185,"Contaminant":"False","Aligned.m.z..Da.":406.21838,"MH...Da.":811.42973,"Theo..MH...Da.":811.43084,"DeltaM..ppm.":-1.37,"Deltam.z..Da.":-0.00056,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2014454,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":56.16315,"Ion.Inject.Time..ms.":16.155,"RT..min.":4.4628,"First.Scan":1276,"Last.Scan":1276,"Master.Scan.s.":1275,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_02.raw","File.ID":"F14","Quan.Info":"","Peptides.Matched":181,"XCorr":2.28,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":0.00015,"Percolator.SVMScore":1.45,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (W4)","Search.ID":"W","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"AAEAGETGAATSATEGDNNNNTAAGDKK","Annotated.Sequence":"[K].AAEAGETGAATsATEGDNNNNTAAGDKK.[G]","Modifications":"S12(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0475,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":906.04937,"Contaminant":"False","Aligned.m.z..Da.":906.04923,"MH...Da.":2716.13357,"Theo..MH...Da.":2716.13298,"DeltaM..ppm.":0.22,"Deltam.z..Da.":0.0002,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1993297.875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":6.33857,"Ion.Inject.Time..ms.":22,"RT..min.":5.0056,"First.Scan":1803,"Last.Scan":1803,"Master.Scan.s.":1795,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_02.raw","File.ID":"F14","Quan.Info":"","Peptides.Matched":2565,"XCorr":6.53,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":6.058e-16,"Percolator.SVMScore":7.965,"ptmRS..Binomial.Peptide.Score":499.85,"ptmRS..Isoform.Confidence.Probability":0.992,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(17): 1688.75; y2+-H(3) O(4) P(22): 1045.47; y2+-H(3) O(4) P(18): 895.40; y2+-H(3) O(4) P(17): 844.88; b1+(7): 630.27","ptmRS..Best.Site.Probabilities":"S12(Phospho): 99.21","ptmRS..Phospho.Site.Probabilities":"T(7): 0.8; T(11): 0.0; S(12): 99.2; T(14): 0.0; T(22): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (W4)","Search.ID":"W","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LQQTAFDKK","Annotated.Sequence":"[R].LQQTAFDKK.[S]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":4,"DeltaScore":0.3105,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":360.20143,"Contaminant":"False","Aligned.m.z..Da.":360.20139,"MH...Da.":1078.58974,"Theo..MH...Da.":1078.58914,"DeltaM..ppm.":0.56,"Deltam.z..Da.":0.0002,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1884894.125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":59.637,"Ion.Inject.Time..ms.":22,"RT..min.":5.4816,"First.Scan":2277,"Last.Scan":2277,"Master.Scan.s.":2276,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_02.raw","File.ID":"F14","Quan.Info":"","Peptides.Matched":392,"XCorr":2.19,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":0.00132,"Percolator.SVMScore":1.02,"Precursor.Area":1252051.625,"Apex.RT..min.":5.48,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (W4)","Search.ID":"W","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KDELTLEGIK","Annotated.Sequence":"[K].KDELTLEGIK.[Q]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.5439,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":382.55171,"Contaminant":"False","Aligned.m.z..Da.":382.55173,"MH...Da.":1145.64059,"Theo..MH...Da.":1145.64123,"DeltaM..ppm.":-0.56,"Deltam.z..Da.":-0.00021,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1344793.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":34.15526,"Ion.Inject.Time..ms.":22,"RT..min.":10.248,"First.Scan":8645,"Last.Scan":8645,"Master.Scan.s.":8633,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_02.raw","File.ID":"F14","Quan.Info":"","Peptides.Matched":314,"XCorr":2.85,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":0.00029,"Percolator.SVMScore":1.324,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (W4)","Search.ID":"W","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KDELTLEGIK","Annotated.Sequence":"[K].KDELTLEGIK.[Q]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.512,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":573.32418,"Contaminant":"False","Aligned.m.z..Da.":573.3242,"MH...Da.":1145.64109,"Theo..MH...Da.":1145.64123,"DeltaM..ppm.":-0.13,"Deltam.z..Da.":-0.00007,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2607419.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":2.08491,"Ion.Inject.Time..ms.":22,"RT..min.":10.2539,"First.Scan":8651,"Last.Scan":8651,"Master.Scan.s.":8646,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_02.raw","File.ID":"F14","Quan.Info":"","Peptides.Matched":307,"XCorr":3.75,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":9.865e-09,"Percolator.SVMScore":3.364,"Precursor.Area":1488862.125,"Apex.RT..min.":10.25,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (W4)","Search.ID":"W","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"APQALMLAPTR","Annotated.Sequence":"[K].APQALmLAPTR.[E]","Modifications":"M6(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7185,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":592.82584,"Contaminant":"False","Aligned.m.z..Da.":592.82587,"MH...Da.":1184.64441,"Theo..MH...Da.":1184.6456,"DeltaM..ppm.":-1.01,"Deltam.z..Da.":-0.0006,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":993155.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":28.97412,"Ion.Inject.Time..ms.":22,"RT..min.":10.1749,"First.Scan":8553,"Last.Scan":8553,"Master.Scan.s.":8542,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_02.raw","File.ID":"F14","Quan.Info":"","Peptides.Matched":333,"XCorr":3.02,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":6.885e-06,"Percolator.SVMScore":2.066,"Precursor.Area":1630835.125,"Apex.RT..min.":10.19,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (W4)","Search.ID":"W","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KFQDDTLNR","Annotated.Sequence":"[K].KFQDDtLNR.[V]","Modifications":"T6(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6554,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":608.7721,"Contaminant":"False","Aligned.m.z..Da.":608.77203,"MH...Da.":1216.53693,"Theo..MH...Da.":1216.53579,"DeltaM..ppm.":0.94,"Deltam.z..Da.":0.00057,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2028491.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":17.56891,"Ion.Inject.Time..ms.":22,"RT..min.":6.1936,"First.Scan":3168,"Last.Scan":3168,"Master.Scan.s.":3160,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_02.raw","File.ID":"F14","Quan.Info":"","Peptides.Matched":614,"XCorr":2.96,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":0.00004,"Percolator.SVMScore":1.706,"Precursor.Area":1417699.5,"Apex.RT..min.":6.2,"ptmRS..Binomial.Peptide.Score":358.11,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"T6(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"T(6): 100.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (W4)","Search.ID":"W","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].mSEGITDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6693,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1056.48524,"Contaminant":"False","Aligned.m.z..Da.":1056.48529,"MH...Da.":2111.96321,"Theo..MH...Da.":2111.96151,"DeltaM..ppm.":0.81,"Deltam.z..Da.":0.00085,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":247799.98438,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":93.0836,"Ion.Inject.Time..ms.":22,"RT..min.":14.6431,"First.Scan":14320,"Last.Scan":14320,"Master.Scan.s.":14314,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_02.raw","File.ID":"F14","Quan.Info":"","Peptides.Matched":2364,"XCorr":3.87,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":1.893e-11,"Percolator.SVMScore":4.605,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (W4)","Search.ID":"W","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":4,"DeltaScore":0.0468,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40613,"Contaminant":"False","Aligned.m.z..Da.":967.40615,"MH...Da.":2900.20384,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":1.55,"Deltam.z..Da.":0.0015,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":4382213.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":52.23455,"Ion.Inject.Time..ms.":22,"RT..min.":15.1694,"First.Scan":14941,"Last.Scan":14941,"Master.Scan.s.":14936,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_02.raw","File.ID":"F14","Quan.Info":"","Peptides.Matched":2579,"XCorr":5.13,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":2.941e-10,"Percolator.SVMScore":4.061,"Precursor.Area":18746150,"Apex.RT..min.":15.08,"ptmRS..Binomial.Peptide.Score":544.79,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(14): 1528.61; b1+-H(3) O(4) P(15): 1642.65; b1+-H(3) O(4) P(16): 1771.69; y1+-H(3) O(4) P(14): 1621.73; y1+-H(3) O(4) P(13): 1458.67; b2+-H(3) O(4) P(16): 886.35; b2+-H(3) O(4) P(18): 1014.90; y2+-H(3) O(4) P(14): 811.37; b1+(12): 1344.56","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (W4)","Search.ID":"W","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":5,"DeltaScore":0.0567,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40472,"Contaminant":"False","Aligned.m.z..Da.":967.40474,"MH...Da.":2900.19962,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":0.1,"Deltam.z..Da.":0.0001,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2609737,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":24.84557,"Ion.Inject.Time..ms.":22,"RT..min.":15.1843,"First.Scan":14960,"Last.Scan":14960,"Master.Scan.s.":14949,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_02.raw","File.ID":"F14","Quan.Info":"","Peptides.Matched":2465,"XCorr":6.53,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":3.996e-14,"Percolator.SVMScore":5.826,"Precursor.Area":18746150,"Apex.RT..min.":15.08,"ptmRS..Binomial.Peptide.Score":537.38,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(14): 1528.61; y1+-H(3) O(4) P(14): 1621.73; y1+-H(3) O(4) P(13): 1458.67; b2+-H(3) O(4) P(18): 1014.90; y2+-H(3) O(4) P(14): 811.37; b1+(12): 1344.56","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (W4)","Search.ID":"W","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":5,"DeltaScore":0.0798,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40412,"Contaminant":"False","Aligned.m.z..Da.":967.40415,"MH...Da.":2900.19781,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":-0.52,"Deltam.z..Da.":-0.00051,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":11644528,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":3.84341,"Ion.Inject.Time..ms.":14.107,"RT..min.":15.1091,"First.Scan":14869,"Last.Scan":14869,"Master.Scan.s.":14858,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_02.raw","File.ID":"F14","Quan.Info":"","Peptides.Matched":2423,"XCorr":6.14,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":6.226e-16,"Percolator.SVMScore":7.74,"Precursor.Area":18746150,"Apex.RT..min.":15.08,"ptmRS..Binomial.Peptide.Score":819.98,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(13): 1413.58; b1+-H(3) O(4) P(14): 1528.61; b1+-H(3) O(4) P(18): 2028.79; y1+-H(3) O(4) P(15): 1750.78; y1+-H(3) O(4) P(14): 1621.73; y1+-H(3) O(4) P(13): 1458.67; b2+-H(3) O(4) P(16): 886.35; b2+-H(3) O(4) P(17): 950.38; b2+-H(3) O(4) P(18): 1014.90; y2+-H(3) O(4) P(14): 811.37; y2+-H(3) O(4) P(13): 729.84; b1+(12): 1344.56; y1+(13): 1556.65","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (W4)","Search.ID":"W","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":5,"DeltaScore":0.075,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40412,"Contaminant":"False","Aligned.m.z..Da.":967.40415,"MH...Da.":2900.19781,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":-0.52,"Deltam.z..Da.":-0.00051,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":11644528,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":2.29111,"Ion.Inject.Time..ms.":9.904,"RT..min.":15.1044,"First.Scan":14862,"Last.Scan":14862,"Master.Scan.s.":14858,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_02.raw","File.ID":"F14","Quan.Info":"","Peptides.Matched":2423,"XCorr":6.67,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":1.363e-15,"Percolator.SVMScore":6.496,"Precursor.Area":18746150,"Apex.RT..min.":15.08,"ptmRS..Binomial.Peptide.Score":743.46,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(13): 1413.58; b1+-H(3) O(4) P(14): 1528.61; b1+-H(3) O(4) P(15): 1642.65; b1+-H(3) O(4) P(18): 2028.79; y1+-H(3) O(4) P(15): 1750.78; y1+-H(3) O(4) P(14): 1621.73; y1+-H(3) O(4) P(13): 1458.67; b2+-H(3) O(4) P(16): 886.35; b2+-H(3) O(4) P(18): 1014.90; y2+-H(3) O(4) P(14): 811.37; b1+(12): 1344.56; y1+(13): 1556.65","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (W4)","Search.ID":"W","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.4021,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40657,"Contaminant":"False","Aligned.m.z..Da.":967.40661,"MH...Da.":2900.20518,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":2.02,"Deltam.z..Da.":0.00195,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":285681.0625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":7.19032,"Ion.Inject.Time..ms.":22,"RT..min.":14.9921,"First.Scan":14724,"Last.Scan":14724,"Master.Scan.s.":14715,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_02.raw","File.ID":"F14","Quan.Info":"","Peptides.Matched":2545,"XCorr":3.73,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":9.528e-08,"Percolator.SVMScore":2.915,"Precursor.Area":18746150,"Apex.RT..min.":15.08,"ptmRS..Binomial.Peptide.Score":295,"ptmRS..Isoform.Confidence.Probability":0.991,"ptmRS..Isoform.Group.Report":"b2+-H(3) O(4) P(16): 886.35","ptmRS..Best.Site.Probabilities":"S13(Phospho): 99.1","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.9; S(13): 99.1; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (W4)","Search.ID":"W","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FTVSAIYSDLPQQER","Annotated.Sequence":"[K].FTVSAIYSDLPQQER.[D]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6883,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":877.4411,"Contaminant":"False","Aligned.m.z..Da.":877.44113,"MH...Da.":1753.87492,"Theo..MH...Da.":1753.87554,"DeltaM..ppm.":-0.35,"Deltam.z..Da.":-0.00031,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":529256.0625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":20.28593,"Ion.Inject.Time..ms.":22,"RT..min.":15.0211,"First.Scan":14759,"Last.Scan":14759,"Master.Scan.s.":14754,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_02.raw","File.ID":"F14","Quan.Info":"","Peptides.Matched":1124,"XCorr":3.69,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":2.352e-10,"Percolator.SVMScore":4.105,"Precursor.Area":478164,"Apex.RT..min.":15.02,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (W4)","Search.ID":"W","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"GVFGYGFEEPSAIQQR","Annotated.Sequence":"[R].GVFGYGFEEPSAIQQR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6917,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":892.93431,"Contaminant":"False","Aligned.m.z..Da.":892.93435,"MH...Da.":1784.86135,"Theo..MH...Da.":1784.86022,"DeltaM..ppm.":0.63,"Deltam.z..Da.":0.00057,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":467645,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":17.80286,"Ion.Inject.Time..ms.":22,"RT..min.":15.0118,"First.Scan":14748,"Last.Scan":14748,"Master.Scan.s.":14741,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_02.raw","File.ID":"F14","Quan.Info":"","Peptides.Matched":1386,"XCorr":3.86,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":4.399e-12,"Percolator.SVMScore":4.894,"Precursor.Area":617442.625,"Apex.RT..min.":15.02,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (W4)","Search.ID":"W","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"TGTFSIAALQR","Annotated.Sequence":"[K].TGTFSIAALQR.[I]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7598,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":582.82253,"Contaminant":"False","Aligned.m.z..Da.":582.82252,"MH...Da.":1164.63779,"Theo..MH...Da.":1164.63715,"DeltaM..ppm.":0.55,"Deltam.z..Da.":0.00032,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1474816.375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":7.13158,"Ion.Inject.Time..ms.":22,"RT..min.":13.3785,"First.Scan":12755,"Last.Scan":12755,"Master.Scan.s.":12754,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_02.raw","File.ID":"F14","Quan.Info":"","Peptides.Matched":360,"XCorr":3.33,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":1.204e-06,"Percolator.SVMScore":2.412,"Precursor.Area":1052009.875,"Apex.RT..min.":13.38,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (W4)","Search.ID":"W","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"HSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[R].HSDSySENETNHTNVPISSTGGTNNK.[T]","Modifications":"Y5(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":0,"Charge":4,"Original.Precursor.Charge":4,"DeltaScore":0.0033,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":718.30201,"Contaminant":"False","Aligned.m.z..Da.":718.30208,"MH...Da.":2870.18623,"Theo..MH...Da.":2870.18608,"DeltaM..ppm.":0.05,"Deltam.z..Da.":0.00004,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1571072,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":7.6475,"First.Scan":5445,"Last.Scan":5445,"Master.Scan.s.":5435,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_02.raw","File.ID":"F14","Quan.Info":"","Peptides.Matched":2498,"XCorr":6.09,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":1.615e-10,"Percolator.SVMScore":4.18,"Precursor.Area":3803159.25,"Apex.RT..min.":7.66,"ptmRS..Binomial.Peptide.Score":262.58,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(6): 659.24; b1+-H(3) O(4) P(9): 1031.37; b1+-H(3) O(4) P(11): 1246.46; b2+-H(3) O(4) P(7): 394.65; b2+-H(3) O(4) P(8): 451.67; b2+-H(3) O(4) P(13): 742.79; b2+-H(3) O(4) P(14): 799.81; b2+-H(3) O(4) P(15): 849.34; b2+-H(3) O(4) P(17): 954.41; b1+(4): 427.16","ptmRS..Best.Site.Probabilities":"S6(Phospho): 99.3","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(4): 0.7; Y(5): 0.0; S(6): 99.3; T(10): 0.0; T(13): 0.0; S(18): 0.0; S(19): 0.0; T(20): 0.0; T(23): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (W4)","Search.ID":"W","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DFLLKPELSR","Annotated.Sequence":"[K].DFLLKPELSR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.6053,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":406.56812,"Contaminant":"False","Aligned.m.z..Da.":406.56811,"MH...Da.":1217.68981,"Theo..MH...Da.":1217.68885,"DeltaM..ppm.":0.79,"Deltam.z..Da.":0.00032,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":463364.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":3.00385,"Ion.Inject.Time..ms.":22,"RT..min.":13.1522,"First.Scan":12463,"Last.Scan":12463,"Master.Scan.s.":12455,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_02.raw","File.ID":"F14","Quan.Info":"","Peptides.Matched":265,"XCorr":1.9,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":0.00103,"Percolator.SVMScore":1.071,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (W4)","Search.ID":"W","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DIFSNDELLSDAYDAK","Annotated.Sequence":"[K].DIFSNDELLSDAYDAK.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":3,"DeltaScore":0.7239,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":908.41946,"Contaminant":"False","Aligned.m.z..Da.":908.41911,"MH...Da.":1815.83165,"Theo..MH...Da.":1815.82831,"DeltaM..ppm.":1.84,"Deltam.z..Da.":0.00167,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1984398.375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":1.99654,"Ion.Inject.Time..ms.":22,"RT..min.":17.0972,"First.Scan":17254,"Last.Scan":17254,"Master.Scan.s.":17252,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_02.raw","File.ID":"F14","Quan.Info":"","Peptides.Matched":1703,"XCorr":4.6,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":2.047e-11,"Percolator.SVMScore":4.589,"Precursor.Area":1678452.5,"Apex.RT..min.":17.09,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (W4)","Search.ID":"W","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DIFSNDELLSDAYDAK","Annotated.Sequence":"[K].DIFSNDELLSDAYDAK.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.6065,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":605.94723,"Contaminant":"False","Aligned.m.z..Da.":605.94699,"MH...Da.":1815.82715,"Theo..MH...Da.":1815.82831,"DeltaM..ppm.":-0.64,"Deltam.z..Da.":-0.00039,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":238774.95312,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":7.60774,"Ion.Inject.Time..ms.":22,"RT..min.":17.0757,"First.Scan":17229,"Last.Scan":17229,"Master.Scan.s.":17222,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_02.raw","File.ID":"F14","Quan.Info":"","Peptides.Matched":1638,"XCorr":2.16,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":0.00014,"Percolator.SVMScore":1.469,"Precursor.Area":413437.9375,"Apex.RT..min.":17.09,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (W4)","Search.ID":"W","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0661,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40551,"Contaminant":"False","Aligned.m.z..Da.":967.40514,"MH...Da.":2900.20198,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":0.91,"Deltam.z..Da.":0.00088,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":182153.29688,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":11.28589,"Ion.Inject.Time..ms.":22,"RT..min.":17.1387,"First.Scan":17305,"Last.Scan":17305,"Master.Scan.s.":17299,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_02.raw","File.ID":"F14","Quan.Info":"","Peptides.Matched":2543,"XCorr":3.78,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":2.331e-08,"Percolator.SVMScore":3.194,"ptmRS..Binomial.Peptide.Score":344.79,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(14): 1621.73; b1+(12): 1344.56","ptmRS..Best.Site.Probabilities":"S13(Phospho): 99.99","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (W4)","Search.ID":"W","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.4192,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40631,"Contaminant":"False","Aligned.m.z..Da.":967.40602,"MH...Da.":2900.20438,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":1.74,"Deltam.z..Da.":0.00169,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":138526.10938,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":85.74567,"Ion.Inject.Time..ms.":22,"RT..min.":17.426,"First.Scan":17666,"Last.Scan":17666,"Master.Scan.s.":17655,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_02.raw","File.ID":"F14","Quan.Info":"","Peptides.Matched":2592,"XCorr":2.91,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":2.099e-06,"Percolator.SVMScore":2.302,"ptmRS..Binomial.Peptide.Score":211.93,"ptmRS..Isoform.Confidence.Probability":0.5,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"Y12(Phospho): 50; S13(Phospho): 50","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 50.0; S(13): 50.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (W4)","Search.ID":"W","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"IGNNELLHR","Annotated.Sequence":"[K].IGNNELLHR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q03558","Master.Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","Protein.Accessions":"Q03558","Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.4884,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":355.86523,"Contaminant":"False","Aligned.m.z..Da.":355.86526,"MH...Da.":1065.58115,"Theo..MH...Da.":1065.57997,"DeltaM..ppm.":1.11,"Deltam.z..Da.":0.00039,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1434186.125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":14.84627,"Ion.Inject.Time..ms.":22,"RT..min.":7.5728,"First.Scan":5337,"Last.Scan":5337,"Master.Scan.s.":5331,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_02.raw","File.ID":"F14","Quan.Info":"","Peptides.Matched":307,"XCorr":2.15,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":0.00072,"Percolator.SVMScore":1.142,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (W4)","Search.ID":"W","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"HSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[R].HSDSYsENETNHTNVPISSTGGTNNK.[T]","Modifications":"S6(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0058,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":957.40059,"Contaminant":"False","Aligned.m.z..Da.":957.40067,"MH...Da.":2870.18722,"Theo..MH...Da.":2870.18608,"DeltaM..ppm.":0.4,"Deltam.z..Da.":0.00038,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2150066.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":7.6458,"First.Scan":5442,"Last.Scan":5442,"Master.Scan.s.":5435,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_02.raw","File.ID":"F14","Quan.Info":"","Peptides.Matched":2546,"XCorr":6.87,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":1.666e-15,"Percolator.SVMScore":6.456,"Precursor.Area":7085243.5,"Apex.RT..min.":7.66,"ptmRS..Binomial.Peptide.Score":550.56,"ptmRS..Isoform.Confidence.Probability":0.988,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(6): 659.24; b1+-H(3) O(4) P(11): 1246.46; b1+-H(3) O(4) P(12): 1383.52; b1+-H(3) O(4) P(15): 1697.68; b2+-H(3) O(4) P(12): 692.26; b2+-H(3) O(4) P(13): 742.79; b2+-H(3) O(4) P(14): 799.81; b2+-H(3) O(4) P(15): 849.34; y2+-H(3) O(4) P(25): 1318.08; y2+-H(3) O(4) P(24): 1274.56; y2+-H(3) O(4) P(23): 1217.05; y2+-H(3) O(4) P(21): 1092.00; b1+(4): 427.16","ptmRS..Best.Site.Probabilities":"S6(Phospho): 98.81","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(4): 0.6; Y(5): 0.0; S(6): 98.8; T(10): 0.6; T(13): 0.0; S(18): 0.0; S(19): 0.0; T(20): 0.0; T(23): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (W4)","Search.ID":"W","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LQETNPEEVPK","Annotated.Sequence":"[K].LQETNPEEVPK.[F]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5318,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":642.32906,"Contaminant":"False","Aligned.m.z..Da.":642.32912,"MH...Da.":1283.65086,"Theo..MH...Da.":1283.64777,"DeltaM..ppm.":2.4,"Deltam.z..Da.":0.00154,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1364415.375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":7.67431,"Ion.Inject.Time..ms.":22,"RT..min.":7.6387,"First.Scan":5433,"Last.Scan":5433,"Master.Scan.s.":5422,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_02.raw","File.ID":"F14","Quan.Info":"","Peptides.Matched":542,"XCorr":2.67,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":0.0001,"Percolator.SVMScore":1.526,"Precursor.Area":4268794,"Apex.RT..min.":7.66,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (W4)","Search.ID":"W","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].msEGITDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S2(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1275,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1096.46807,"Contaminant":"False","Aligned.m.z..Da.":1096.46784,"MH...Da.":2191.92888,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":0.47,"Deltam.z..Da.":0.00052,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":109321.74219,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":16.1158,"First.Scan":16076,"Last.Scan":16076,"Master.Scan.s.":16074,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_02.raw","File.ID":"F14","Quan.Info":"","Peptides.Matched":1985,"XCorr":3.06,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":8.58e-08,"Percolator.SVMScore":2.935,"Precursor.Area":187651.26562,"Apex.RT..min.":16.22,"ptmRS..Binomial.Peptide.Score":402.49,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(2): 112.04; b1+-H(3) O(4) P(3): 241.08; b1+-H(3) O(4) P(4): 298.10; b1+-H(3) O(4) P(5): 411.19; b1+-H(3) O(4) P(6): 512.24; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+-H(3) O(4) P(9): 869.39; y1+(14): 1683.77","ptmRS..Best.Site.Probabilities":"S2(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 100.0; T(6): 0.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (W4)","Search.ID":"W","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.3877,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40599,"Contaminant":"False","Aligned.m.z..Da.":967.4058,"MH...Da.":2900.20342,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":1.41,"Deltam.z..Da.":0.00136,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":259991.03125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":16.0524,"First.Scan":15998,"Last.Scan":15998,"Master.Scan.s.":15996,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_02.raw","File.ID":"F14","Quan.Info":"","Peptides.Matched":2580,"XCorr":4.05,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":1.129e-08,"Percolator.SVMScore":3.337,"Precursor.Area":395009.78125,"Apex.RT..min.":16.07,"ptmRS..Binomial.Peptide.Score":369.24,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y2+-H(3) O(4) P(14): 811.37; b1+(12): 1344.56","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (W4)","Search.ID":"W","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FDDMELDENLLR","Annotated.Sequence":"[K].FDDMELDENLLR.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5502,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":755.34661,"Contaminant":"False","Aligned.m.z..Da.":755.34646,"MH...Da.":1509.68594,"Theo..MH...Da.":1509.68898,"DeltaM..ppm.":-2.02,"Deltam.z..Da.":-0.00152,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":336827.28125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":6.58892,"Ion.Inject.Time..ms.":22,"RT..min.":16.0351,"First.Scan":15978,"Last.Scan":15978,"Master.Scan.s.":15970,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_02.raw","File.ID":"F14","Quan.Info":"","Peptides.Matched":1005,"XCorr":2.69,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":6.143e-07,"Percolator.SVMScore":2.545,"Precursor.Area":356955.3125,"Apex.RT..min.":16.04,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (W4)","Search.ID":"W","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].mSEGItDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); T6(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0383,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":731.31397,"Contaminant":"False","Aligned.m.z..Da.":731.31394,"MH...Da.":2191.92737,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":-0.22,"Deltam.z..Da.":-0.00016,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":245667.04688,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":32.73425,"Ion.Inject.Time..ms.":22,"RT..min.":15.5389,"First.Scan":15387,"Last.Scan":15387,"Master.Scan.s.":15379,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_02.raw","File.ID":"F14","Quan.Info":"","Peptides.Matched":1940,"XCorr":2.35,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":3.248e-06,"Percolator.SVMScore":2.215,"Precursor.Area":138905.625,"Apex.RT..min.":15.53,"ptmRS..Binomial.Peptide.Score":232.59,"ptmRS..Isoform.Confidence.Probability":0.993,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+(3): 259.09; b1+(4): 316.11; b1+(5): 429.20","ptmRS..Best.Site.Probabilities":"T6(Phospho): 99.33","ptmRS..Phospho.Site.Probabilities":"S(2): 0.7; T(6): 99.3; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (W4)","Search.ID":"W","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].mSEGItDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); T6(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1237,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1096.46678,"Contaminant":"False","Aligned.m.z..Da.":1096.46673,"MH...Da.":2191.92629,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":-0.71,"Deltam.z..Da.":-0.00078,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":192452.32812,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":15.5375,"First.Scan":15385,"Last.Scan":15385,"Master.Scan.s.":15379,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_02.raw","File.ID":"F14","Quan.Info":"","Peptides.Matched":1957,"XCorr":4.77,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":1.951e-09,"Percolator.SVMScore":3.686,"Precursor.Area":178945.78125,"Apex.RT..min.":15.53,"ptmRS..Binomial.Peptide.Score":397.68,"ptmRS..Isoform.Confidence.Probability":0.996,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(6): 512.24; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+-H(3) O(4) P(9): 869.39; b1+-H(3) O(4) P(10): 998.43; b1+(3): 259.09; b1+(4): 316.11; b1+(5): 429.20","ptmRS..Best.Site.Probabilities":"T6(Phospho): 99.61","ptmRS..Phospho.Site.Probabilities":"S(2): 0.4; T(6): 99.6; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (W4)","Search.ID":"W","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0466,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.4042,"Contaminant":"False","Aligned.m.z..Da.":967.40416,"MH...Da.":2900.19806,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":-0.44,"Deltam.z..Da.":-0.00042,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":470091.4375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":11.76527,"Ion.Inject.Time..ms.":22,"RT..min.":15.5235,"First.Scan":15367,"Last.Scan":15367,"Master.Scan.s.":15366,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_02.raw","File.ID":"F14","Quan.Info":"","Peptides.Matched":2439,"XCorr":4.72,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":2.139e-09,"Percolator.SVMScore":3.667,"Precursor.Area":2396397.5,"Apex.RT..min.":15.37,"ptmRS..Binomial.Peptide.Score":418.13,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(14): 1621.73; b2+-H(3) O(4) P(16): 886.35; y2+-H(3) O(4) P(14): 811.37; b1+(12): 1344.56","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (W4)","Search.ID":"W","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ILISTDLLAR","Annotated.Sequence":"[R].ILISTDLLAR.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5117,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":557.84607,"Contaminant":"False","Aligned.m.z..Da.":557.84604,"MH...Da.":1114.68487,"Theo..MH...Da.":1114.68304,"DeltaM..ppm.":1.64,"Deltam.z..Da.":0.00092,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1396614,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":18.73727,"Ion.Inject.Time..ms.":22,"RT..min.":15.6092,"First.Scan":15460,"Last.Scan":15460,"Master.Scan.s.":15458,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_02.raw","File.ID":"F14","Quan.Info":"","Peptides.Matched":124,"XCorr":2.56,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":0.00004,"Percolator.SVMScore":1.735,"Precursor.Area":1168102.5,"Apex.RT..min.":15.61,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (W4)","Search.ID":"W","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].msEGITDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S2(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":3,"DeltaScore":0.158,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1096.46724,"Contaminant":"False","Aligned.m.z..Da.":1096.46712,"MH...Da.":2191.9272,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":-0.29,"Deltam.z..Da.":-0.00032,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":5963546,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":15.94788,"Ion.Inject.Time..ms.":22,"RT..min.":15.775,"First.Scan":15663,"Last.Scan":15663,"Master.Scan.s.":15653,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_02.raw","File.ID":"F14","Quan.Info":"","Peptides.Matched":1949,"XCorr":3.86,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":3.515e-07,"Percolator.SVMScore":2.656,"Precursor.Area":8508079,"Apex.RT..min.":15.79,"ptmRS..Binomial.Peptide.Score":372.31,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(2): 112.04; b1+-H(3) O(4) P(3): 241.08; b1+-H(3) O(4) P(4): 298.10; b1+-H(3) O(4) P(5): 411.19; b1+-H(3) O(4) P(6): 512.24; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+-H(3) O(4) P(9): 869.39; b1+-H(3) O(4) P(10): 998.43","ptmRS..Best.Site.Probabilities":"S2(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 100.0; T(6): 0.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (W4)","Search.ID":"W","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.3684,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40705,"Contaminant":"False","Aligned.m.z..Da.":967.40669,"MH...Da.":2900.2066,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":2.51,"Deltam.z..Da.":0.00242,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":163774.29688,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":16.6382,"First.Scan":16686,"Last.Scan":16686,"Master.Scan.s.":16677,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_02.raw","File.ID":"F14","Quan.Info":"","Peptides.Matched":2590,"XCorr":3.04,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":2.731e-06,"Percolator.SVMScore":2.249,"ptmRS..Binomial.Peptide.Score":286.39,"ptmRS..Isoform.Confidence.Probability":0.5,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"Y12(Phospho): 50; S13(Phospho): 50","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 50.0; S(13): 50.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (W4)","Search.ID":"W","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"HSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[R].HSDsYsENETNHTNVPISSTGGTNNK.[T]","Modifications":"S4(Phospho); S6(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.1429,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":984.05615,"Contaminant":"False","Aligned.m.z..Da.":984.0563,"MH...Da.":2950.1539,"Theo..MH...Da.":2950.15241,"DeltaM..ppm.":0.51,"Deltam.z..Da.":0.0005,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2401741,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":2.11804,"Ion.Inject.Time..ms.":22,"RT..min.":8.0429,"First.Scan":5969,"Last.Scan":5969,"Master.Scan.s.":5968,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_02.raw","File.ID":"F14","Quan.Info":"","Peptides.Matched":1146,"XCorr":6.44,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":1.913e-11,"Percolator.SVMScore":4.602,"Precursor.Area":2347692,"Apex.RT..min.":8.04,"ptmRS..Binomial.Peptide.Score":554.09,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(6): 739.21; y2+-H(3) O(4) P(22): 1173.53; y2+-H(3) O(4) P(21): 1092.00; b1+- -2H(3) O(4) P -2H(3) O(4) P(6): 641.23; b1+- -2H(3) O(4) P -2H(3) O(4) P(11): 1228.45; b2+- -2H(3) O(4) P -2H(3) O(4) P(13): 733.78; b2+- -2H(3) O(4) P -2H(3) O(4) P(14): 790.80; b2+- -2H(3) O(4) P -2H(3) O(4) P(15): 840.34","ptmRS..Best.Site.Probabilities":"S4(Phospho): 100; S6(Phospho): 99.99","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(4): 100.0; Y(5): 0.0; S(6): 100.0; T(10): 0.0; T(13): 0.0; S(18): 0.0; S(19): 0.0; T(20): 0.0; T(23): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (W4)","Search.ID":"W","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"IQDPQMTGYVSTR","Annotated.Sequence":"[R].IQDPQmtGYVsTR.[Y]","Modifications":"M6(Oxidation); T7(Phospho); S11(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P32485","Master.Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","Protein.Accessions":"P32485","Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.0468,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":836.32937,"Contaminant":"False","Aligned.m.z..Da.":836.32946,"MH...Da.":1671.65147,"Theo..MH...Da.":1671.64853,"DeltaM..ppm.":1.76,"Deltam.z..Da.":0.00147,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1567586.125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":7.7643,"First.Scan":5606,"Last.Scan":5606,"Master.Scan.s.":5604,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_02.raw","File.ID":"F14","Quan.Info":"","Peptides.Matched":726,"XCorr":2.78,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":0.00011,"Percolator.SVMScore":1.516,"Precursor.Area":1361850.125,"Apex.RT..min.":7.75,"ptmRS..Binomial.Peptide.Score":334.77,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(11): 1332.53; y1+-H(3) O(4) P(10): 1217.50; y1+-H(3) O(4) P(8): 992.39; y1+-H(3) O(4) P(7): 845.36; y2+-H(3) O(4) P(11): 666.77; y2+-H(3) O(4) P(10): 609.25; y2+-H(3) O(4) P(8): 496.70; y1+(4): 462.27; y1+(3): 363.20","ptmRS..Best.Site.Probabilities":"T7(Phospho): 100; Y9(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"T(7): 100.0; Y(9): 100.0; S(11): 0.0; T(12): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (W4)","Search.ID":"W","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSSPLHRLSPTGK","Annotated.Sequence":"[R].mSsPLHRLsPTGK.[S]","Modifications":"M1(Oxidation); S3(Phospho); S9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.4725,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":529.56461,"Contaminant":"False","Aligned.m.z..Da.":529.56467,"MH...Da.":1586.67929,"Theo..MH...Da.":1586.67977,"DeltaM..ppm.":-0.3,"Deltam.z..Da.":-0.00016,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3415044.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":22.34501,"Ion.Inject.Time..ms.":22,"RT..min.":7.729,"First.Scan":5559,"Last.Scan":5559,"Master.Scan.s.":5552,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_02.raw","File.ID":"F14","Quan.Info":"","Peptides.Matched":1010,"XCorr":4,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":0.00002,"Percolator.SVMScore":1.833,"Precursor.Area":3675329.25,"Apex.RT..min.":7.74,"ptmRS..Binomial.Peptide.Score":298.61,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(9): 990.58; y1+-H(3) O(4) P(8): 877.50; y1+-H(3) O(4) P(7): 740.44; y2+-H(3) O(4) P(11): 627.82; y2+-H(3) O(4) P(10): 544.32; y2+-H(3) O(4) P(8): 439.25; y1+(4): 402.23; y1+(3): 305.18","ptmRS..Best.Site.Probabilities":"S3(Phospho): 100; S9(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(3): 100.0; S(9): 100.0; T(11): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (W4)","Search.ID":"W","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"AIMPIIEGHDVLAQAQSGTGK","Annotated.Sequence":"[R].AImPIIEGHDVLAQAQSGTGK.[T]","Modifications":"M3(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.7536,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":718.04166,"Contaminant":"False","Aligned.m.z..Da.":718.04165,"MH...Da.":2152.11042,"Theo..MH...Da.":2152.10668,"DeltaM..ppm.":1.74,"Deltam.z..Da.":0.00125,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":428281.21875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":9.03502,"Ion.Inject.Time..ms.":22,"RT..min.":12.75,"First.Scan":11932,"Last.Scan":11932,"Master.Scan.s.":11922,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_02.raw","File.ID":"F14","Quan.Info":"","Peptides.Matched":1005,"XCorr":4.22,"X..Protein.Groups":1,"Percolator.q.Value":0.00014,"Percolator.PEP":3.69e-10,"Percolator.SVMScore":4.016,"Precursor.Area":575522.8125,"Apex.RT..min.":12.76,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AE4)","Search.ID":"AE","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LQETNPEEVPK","Annotated.Sequence":"[K].LQETNPEEVPK.[F]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.4932,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":642.3286,"Contaminant":"False","Aligned.m.z..Da.":642.32864,"MH...Da.":1283.64993,"Theo..MH...Da.":1283.64777,"DeltaM..ppm.":1.68,"Deltam.z..Da.":0.00108,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2011170.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":26.49168,"Ion.Inject.Time..ms.":22,"RT..min.":7.6511,"First.Scan":5765,"Last.Scan":5765,"Master.Scan.s.":5754,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y25_06.raw","File.ID":"F6","Quan.Info":"","Peptides.Matched":517,"XCorr":2.21,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":0.00048,"Percolator.SVMScore":1.124,"Precursor.Area":1382187.875,"Apex.RT..min.":7.64,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AE4)","Search.ID":"AE","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LQETNPEEVPK","Annotated.Sequence":"[K].LQETNPEEVPK.[F]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":3,"DeltaScore":0.3931,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":642.3278,"Contaminant":"False","Aligned.m.z..Da.":642.32784,"MH...Da.":1283.64833,"Theo..MH...Da.":1283.64777,"DeltaM..ppm.":0.44,"Deltam.z..Da.":0.00028,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1645571.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":63.37544,"Ion.Inject.Time..ms.":22,"RT..min.":7.6595,"First.Scan":5776,"Last.Scan":5776,"Master.Scan.s.":5767,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y25_06.raw","File.ID":"F6","Quan.Info":"","Peptides.Matched":575,"XCorr":1.45,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":0.00065,"Percolator.SVMScore":1.069,"Precursor.Area":1382187.875,"Apex.RT..min.":7.64,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AE4)","Search.ID":"AE","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"IQDPQMTGYVSTR","Annotated.Sequence":"[R].IQDPQmTGyVSTR.[Y]","Modifications":"M6(Oxidation); Y9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P32485","Master.Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","Protein.Accessions":"P32485","Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1519,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":796.3461,"Contaminant":"False","Aligned.m.z..Da.":796.34614,"MH...Da.":1591.68493,"Theo..MH...Da.":1591.6822,"DeltaM..ppm.":1.72,"Deltam.z..Da.":0.00137,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2019638.125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":7.623,"First.Scan":5727,"Last.Scan":5727,"Master.Scan.s.":5715,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y25_06.raw","File.ID":"F6","Quan.Info":"","Peptides.Matched":1189,"XCorr":2.83,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":2.321e-06,"Percolator.SVMScore":2.112,"Precursor.Area":1393556.125,"Apex.RT..min.":7.62,"ptmRS..Binomial.Peptide.Score":277.24,"ptmRS..Isoform.Confidence.Probability":0.988,"ptmRS..Isoform.Group.Report":"y1+(6): 762.32; y1+(5): 705.30","ptmRS..Best.Site.Probabilities":"Y9(Phospho): 98.79","ptmRS..Phospho.Site.Probabilities":"T(7): 0.4; Y(9): 98.8; S(11): 0.4; T(12): 0.4"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AE4)","Search.ID":"AE","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSSPLHRLSPTGK","Annotated.Sequence":"[R].mSsPLHRLsPTGK.[S]","Modifications":"M1(Oxidation); S3(Phospho); S9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.3655,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":529.56529,"Contaminant":"False","Aligned.m.z..Da.":529.56533,"MH...Da.":1586.68132,"Theo..MH...Da.":1586.67977,"DeltaM..ppm.":0.98,"Deltam.z..Da.":0.00052,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1850488.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":51.46473,"Ion.Inject.Time..ms.":22,"RT..min.":7.7178,"First.Scan":5855,"Last.Scan":5855,"Master.Scan.s.":5845,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y25_06.raw","File.ID":"F6","Quan.Info":"","Peptides.Matched":1141,"XCorr":2.9,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":0.00024,"Percolator.SVMScore":1.256,"ptmRS..Binomial.Peptide.Score":191.04,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(8): 877.50; y2+-H(3) O(4) P(10): 544.32; y2+-H(3) O(4) P(8): 439.25; y1+(4): 402.23; y1+(3): 305.18","ptmRS..Best.Site.Probabilities":"S3(Phospho): 100; S9(Phospho): 99.99","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(3): 100.0; S(9): 100.0; T(11): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AE4)","Search.ID":"AE","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"TGTFSIAALQR","Annotated.Sequence":"[K].TGTFSIAALQR.[I]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":3,"DeltaScore":0.6667,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":582.82135,"Contaminant":"False","Aligned.m.z..Da.":582.82132,"MH...Da.":1164.63542,"Theo..MH...Da.":1164.63715,"DeltaM..ppm.":-1.48,"Deltam.z..Da.":-0.00086,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":148647.01562,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":77.99617,"Ion.Inject.Time..ms.":22,"RT..min.":13.4226,"First.Scan":13491,"Last.Scan":13491,"Master.Scan.s.":13488,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y25_06.raw","File.ID":"F6","Quan.Info":"","Peptides.Matched":371,"XCorr":1.86,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":0.00014,"Percolator.SVMScore":1.35,"Precursor.Area":20756894,"Apex.RT..min.":13.32,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AE4)","Search.ID":"AE","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DIFSNDELLSDAYDAK","Annotated.Sequence":"[K].DIFSNDELLSDAYDAK.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7668,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":908.42044,"Contaminant":"False","Aligned.m.z..Da.":908.42048,"MH...Da.":1815.83361,"Theo..MH...Da.":1815.82831,"DeltaM..ppm.":2.91,"Deltam.z..Da.":0.00265,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":377473.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":17.0974,"First.Scan":17134,"Last.Scan":17134,"Master.Scan.s.":17131,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y25_06.raw","File.ID":"F6","Quan.Info":"","Peptides.Matched":1746,"XCorr":3.43,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":3.783e-08,"Percolator.SVMScore":2.874,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AE4)","Search.ID":"AE","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":5,"DeltaScore":0.0653,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40496,"Contaminant":"False","Aligned.m.z..Da.":967.40514,"MH...Da.":2900.20034,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":0.35,"Deltam.z..Da.":0.00034,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2286418.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":4.77735,"Ion.Inject.Time..ms.":22,"RT..min.":15.0096,"First.Scan":15162,"Last.Scan":15162,"Master.Scan.s.":15158,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y25_06.raw","File.ID":"F6","Quan.Info":"","Peptides.Matched":2493,"XCorr":7.04,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":1.579e-15,"Percolator.SVMScore":6.324,"Precursor.Area":2827198.5,"Apex.RT..min.":15.01,"ptmRS..Binomial.Peptide.Score":749.6,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(13): 1413.58; b1+-H(3) O(4) P(14): 1528.61; b1+-H(3) O(4) P(15): 1642.65; b1+-H(3) O(4) P(16): 1771.69; b1+-H(3) O(4) P(18): 2028.79; y1+-H(3) O(4) P(15): 1750.78; y1+-H(3) O(4) P(14): 1621.73; y1+-H(3) O(4) P(13): 1458.67; b2+-H(3) O(4) P(16): 886.35; b2+-H(3) O(4) P(17): 950.38; b2+-H(3) O(4) P(18): 1014.90; y2+-H(3) O(4) P(14): 811.37; b1+(12): 1344.56; y1+(13): 1556.65","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AE4)","Search.ID":"AE","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.3683,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40339,"Contaminant":"False","Aligned.m.z..Da.":967.40357,"MH...Da.":2900.19562,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":-1.28,"Deltam.z..Da.":-0.00124,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":100573.58594,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":23.63372,"Ion.Inject.Time..ms.":22,"RT..min.":14.9539,"First.Scan":15109,"Last.Scan":15109,"Master.Scan.s.":15106,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y25_06.raw","File.ID":"F6","Quan.Info":"","Peptides.Matched":2273,"XCorr":4.48,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":6.315e-09,"Percolator.SVMScore":3.206,"Precursor.Area":2827198.5,"Apex.RT..min.":15.01,"ptmRS..Binomial.Peptide.Score":360.9,"ptmRS..Isoform.Confidence.Probability":0.5,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"Y12(Phospho): 50; S13(Phospho): 50","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 50.0; S(13): 50.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AE4)","Search.ID":"AE","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"GVFGYGFEEPSAIQQR","Annotated.Sequence":"[R].GVFGYGFEEPSAIQQR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6804,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":892.93634,"Contaminant":"False","Aligned.m.z..Da.":892.93649,"MH...Da.":1784.8654,"Theo..MH...Da.":1784.86022,"DeltaM..ppm.":2.9,"Deltam.z..Da.":0.00259,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":248398.67188,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":37.74239,"Ion.Inject.Time..ms.":22,"RT..min.":15.0701,"First.Scan":15228,"Last.Scan":15228,"Master.Scan.s.":15227,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y25_06.raw","File.ID":"F6","Quan.Info":"","Peptides.Matched":1547,"XCorr":2.91,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":4.305e-08,"Percolator.SVMScore":2.85,"Precursor.Area":146647.40625,"Apex.RT..min.":15.07,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AE4)","Search.ID":"AE","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].msEGITDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S2(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0912,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":731.31725,"Contaminant":"False","Aligned.m.z..Da.":731.3172,"MH...Da.":2191.93721,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":4.27,"Deltam.z..Da.":0.00312,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":186313.21875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":4.7998,"Ion.Inject.Time..ms.":22,"RT..min.":15.651,"First.Scan":15901,"Last.Scan":15901,"Master.Scan.s.":15898,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y25_06.raw","File.ID":"F6","Quan.Info":"","Peptides.Matched":2135,"XCorr":2.85,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":0.0023,"Percolator.SVMScore":0.833,"ptmRS..Binomial.Peptide.Score":309.58,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(2): 112.04; b1+-H(3) O(4) P(3): 241.08; b1+-H(3) O(4) P(4): 298.10; b1+-H(3) O(4) P(5): 411.19; b1+-H(3) O(4) P(6): 512.24; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+-H(3) O(4) P(9): 869.39; b1+-H(3) O(4) P(10): 998.43","ptmRS..Best.Site.Probabilities":"S2(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 100.0; T(6): 0.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AE4)","Search.ID":"AE","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].msEGITDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S2(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1766,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1096.46276,"Contaminant":"False","Aligned.m.z..Da.":1096.46271,"MH...Da.":2191.91825,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":-4.38,"Deltam.z..Da.":-0.0048,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":481171.46875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":15.6152,"First.Scan":15867,"Last.Scan":15867,"Master.Scan.s.":15863,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y25_06.raw","File.ID":"F6","Quan.Info":"","Peptides.Matched":1872,"XCorr":4.36,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":2.846e-10,"Percolator.SVMScore":3.78,"ptmRS..Binomial.Peptide.Score":516.3,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(2): 112.04; b1+-H(3) O(4) P(3): 241.08; b1+-H(3) O(4) P(4): 298.10; b1+-H(3) O(4) P(5): 411.19; b1+-H(3) O(4) P(6): 512.24; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+-H(3) O(4) P(9): 869.39; b1+-H(3) O(4) P(10): 998.43; b1+-H(3) O(4) P(13): 1326.61; y1+(14): 1683.77","ptmRS..Best.Site.Probabilities":"S2(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 100.0; T(6): 0.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AE4)","Search.ID":"AE","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.4349,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40165,"Contaminant":"False","Aligned.m.z..Da.":967.40169,"MH...Da.":2900.19042,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":-3.07,"Deltam.z..Da.":-0.00297,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":137742.90625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":58.34895,"Ion.Inject.Time..ms.":22,"RT..min.":15.4449,"First.Scan":15696,"Last.Scan":15696,"Master.Scan.s.":15694,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y25_06.raw","File.ID":"F6","Quan.Info":"","Peptides.Matched":2104,"XCorr":2.69,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":8.868e-06,"Percolator.SVMScore":1.864,"Precursor.Area":650142.9375,"Apex.RT..min.":15.34,"ptmRS..Binomial.Peptide.Score":266,"ptmRS..Isoform.Confidence.Probability":0.5,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"Y12(Phospho): 50; S13(Phospho): 50","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 50.0; S(13): 50.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AE4)","Search.ID":"AE","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KDELTLEGIK","Annotated.Sequence":"[K].KDELTLEGIK.[Q]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5563,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":573.32467,"Contaminant":"False","Aligned.m.z..Da.":573.32458,"MH...Da.":1145.64207,"Theo..MH...Da.":1145.64123,"DeltaM..ppm.":0.73,"Deltam.z..Da.":0.00042,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":731482.0625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":13.07582,"Ion.Inject.Time..ms.":22,"RT..min.":10.3086,"First.Scan":9402,"Last.Scan":9402,"Master.Scan.s.":9394,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y25_06.raw","File.ID":"F6","Quan.Info":"","Peptides.Matched":302,"XCorr":2.84,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":6.4e-08,"Percolator.SVMScore":2.777,"Precursor.Area":467635.625,"Apex.RT..min.":10.3,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AE4)","Search.ID":"AE","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ELALQIQK","Annotated.Sequence":"[R].ELALQIQK.[V]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.2087,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":471.785,"Contaminant":"False","Aligned.m.z..Da.":471.78496,"MH...Da.":942.56272,"Theo..MH...Da.":942.56186,"DeltaM..ppm.":0.92,"Deltam.z..Da.":0.00043,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":502060.03125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":24.06512,"Ion.Inject.Time..ms.":22,"RT..min.":10.6525,"First.Scan":9881,"Last.Scan":9881,"Master.Scan.s.":9875,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y25_06.raw","File.ID":"F6","Quan.Info":"","Peptides.Matched":292,"XCorr":2.06,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":0.00159,"Percolator.SVMScore":0.902,"Precursor.Area":495769.34375,"Apex.RT..min.":10.66,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Z4)","Search.ID":"Z","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"IQDPQMTGYVSTR","Annotated.Sequence":"[R].IQDPQMTGyVSTR.[Y]","Modifications":"Y9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P32485","Master.Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","Protein.Accessions":"P32485","Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1046,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":788.34548,"Contaminant":"False","Aligned.m.z..Da.":788.34554,"MH...Da.":1575.68369,"Theo..MH...Da.":1575.68728,"DeltaM..ppm.":-2.28,"Deltam.z..Da.":-0.0018,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1900880.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":9.5834,"First.Scan":7823,"Last.Scan":7823,"Master.Scan.s.":7822,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_05.raw","File.ID":"F11","Quan.Info":"","Peptides.Matched":1090,"XCorr":3.06,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":5.333e-07,"Percolator.SVMScore":2.364,"Precursor.Area":1260545.125,"Apex.RT..min.":9.58,"ptmRS..Binomial.Peptide.Score":289.34,"ptmRS..Isoform.Confidence.Probability":0.25,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(11): 1236.57; y1+-H(3) O(4) P(10): 1121.54","ptmRS..Best.Site.Probabilities":"T7(Phospho): 25; Y9(Phospho): 25; S11(Phospho): 25; T12(Phospho): 25","ptmRS..Phospho.Site.Probabilities":"T(7): 25.0; Y(9): 25.0; S(11): 25.0; T(12): 25.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Z4)","Search.ID":"Z","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ELALQIQK","Annotated.Sequence":"[R].ELALQIQK.[V]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.2411,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":471.78461,"Contaminant":"False","Aligned.m.z..Da.":471.78463,"MH...Da.":942.56194,"Theo..MH...Da.":942.56186,"DeltaM..ppm.":0.09,"Deltam.z..Da.":0.00004,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":933535.8125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":8.35504,"Ion.Inject.Time..ms.":22,"RT..min.":10.5691,"First.Scan":9063,"Last.Scan":9063,"Master.Scan.s.":9057,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_05.raw","File.ID":"F11","Quan.Info":"","Peptides.Matched":292,"XCorr":2.24,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":0.00015,"Percolator.SVMScore":1.3,"Precursor.Area":904337.75,"Apex.RT..min.":10.58,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Z4)","Search.ID":"Z","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"APQALMLAPTR","Annotated.Sequence":"[K].APQALmLAPTR.[E]","Modifications":"M6(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6926,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":592.82681,"Contaminant":"False","Aligned.m.z..Da.":592.82685,"MH...Da.":1184.64635,"Theo..MH...Da.":1184.6456,"DeltaM..ppm.":0.63,"Deltam.z..Da.":0.00037,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1068712.625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":19.97825,"Ion.Inject.Time..ms.":22,"RT..min.":10.1875,"First.Scan":8574,"Last.Scan":8574,"Master.Scan.s.":8563,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_05.raw","File.ID":"F11","Quan.Info":"","Peptides.Matched":320,"XCorr":2.83,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":5.801e-06,"Percolator.SVMScore":1.915,"Precursor.Area":839173.375,"Apex.RT..min.":10.19,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Z4)","Search.ID":"Z","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KDELTLEGIK","Annotated.Sequence":"[K].KDELTLEGIK.[Q]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.5063,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":382.55182,"Contaminant":"False","Aligned.m.z..Da.":382.55184,"MH...Da.":1145.64091,"Theo..MH...Da.":1145.64123,"DeltaM..ppm.":-0.28,"Deltam.z..Da.":-0.00011,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1321832,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":41.87005,"Ion.Inject.Time..ms.":22,"RT..min.":10.2549,"First.Scan":8658,"Last.Scan":8658,"Master.Scan.s.":8654,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_05.raw","File.ID":"F11","Quan.Info":"","Peptides.Matched":311,"XCorr":2.37,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":0.00003,"Percolator.SVMScore":1.593,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Z4)","Search.ID":"Z","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KDELTLEGIK","Annotated.Sequence":"[K].KDELTLEGIK.[Q]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5042,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":573.32474,"Contaminant":"False","Aligned.m.z..Da.":573.32477,"MH...Da.":1145.64221,"Theo..MH...Da.":1145.64123,"DeltaM..ppm.":0.85,"Deltam.z..Da.":0.00049,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1217560.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":3.70031,"Ion.Inject.Time..ms.":22,"RT..min.":10.2568,"First.Scan":8661,"Last.Scan":8661,"Master.Scan.s.":8654,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_05.raw","File.ID":"F11","Quan.Info":"","Peptides.Matched":301,"XCorr":3.53,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":1.033e-10,"Percolator.SVMScore":3.971,"Precursor.Area":788999.25,"Apex.RT..min.":10.25,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Z4)","Search.ID":"Z","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"HSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[R].HSDSySENETNHTNVPISSTGGTNNK.[T]","Modifications":"Y5(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":0,"Charge":4,"Original.Precursor.Charge":4,"DeltaScore":0.0074,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":718.30302,"Contaminant":"False","Aligned.m.z..Da.":718.30304,"MH...Da.":2870.19027,"Theo..MH...Da.":2870.18608,"DeltaM..ppm.":1.46,"Deltam.z..Da.":0.00105,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1871497.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":2.25899,"Ion.Inject.Time..ms.":22,"RT..min.":7.6534,"First.Scan":5453,"Last.Scan":5453,"Master.Scan.s.":5443,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_05.raw","File.ID":"F11","Quan.Info":"","Peptides.Matched":2537,"XCorr":5.39,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":1.235e-09,"Percolator.SVMScore":3.505,"Precursor.Area":2130348.5,"Apex.RT..min.":7.66,"ptmRS..Binomial.Peptide.Score":258.31,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(9): 1031.37; b1+-H(3) O(4) P(11): 1246.46; b2+-H(3) O(4) P(7): 394.65; b2+-H(3) O(4) P(13): 742.79; b2+-H(3) O(4) P(14): 799.81; b2+-H(3) O(4) P(15): 849.34; b2+-H(3) O(4) P(17): 954.41; b1+(4): 427.16","ptmRS..Best.Site.Probabilities":"S6(Phospho): 99.18","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(4): 0.8; Y(5): 0.0; S(6): 99.2; T(10): 0.0; T(13): 0.0; S(18): 0.0; S(19): 0.0; T(20): 0.0; T(23): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Z4)","Search.ID":"Z","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"HSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[R].HSDSYsENETNHTNVPISSTGGTNNK.[T]","Modifications":"S6(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0098,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":957.40078,"Contaminant":"False","Aligned.m.z..Da.":957.4008,"MH...Da.":2870.18778,"Theo..MH...Da.":2870.18608,"DeltaM..ppm.":0.59,"Deltam.z..Da.":0.00057,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3587395,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":7.6494,"First.Scan":5446,"Last.Scan":5446,"Master.Scan.s.":5443,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_05.raw","File.ID":"F11","Quan.Info":"","Peptides.Matched":2498,"XCorr":6.1,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":1.858e-14,"Percolator.SVMScore":5.593,"Precursor.Area":4001770.75,"Apex.RT..min.":7.66,"ptmRS..Binomial.Peptide.Score":520.74,"ptmRS..Isoform.Confidence.Probability":0.993,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(6): 659.24; b1+-H(3) O(4) P(11): 1246.46; b2+-H(3) O(4) P(8): 451.67; b2+-H(3) O(4) P(12): 692.26; b2+-H(3) O(4) P(13): 742.79; b2+-H(3) O(4) P(14): 799.81; b2+-H(3) O(4) P(15): 849.34; y2+-H(3) O(4) P(25): 1318.08; y2+-H(3) O(4) P(24): 1274.56; y2+-H(3) O(4) P(23): 1217.05; y2+-H(3) O(4) P(21): 1092.00; b1+(4): 427.16","ptmRS..Best.Site.Probabilities":"S6(Phospho): 99.33","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(4): 0.7; Y(5): 0.0; S(6): 99.3; T(10): 0.0; T(13): 0.0; S(18): 0.0; S(19): 0.0; T(20): 0.0; T(23): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Z4)","Search.ID":"Z","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LQETNPEEVPK","Annotated.Sequence":"[K].LQETNPEEVPK.[F]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5738,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":642.32809,"Contaminant":"False","Aligned.m.z..Da.":642.3281,"MH...Da.":1283.6489,"Theo..MH...Da.":1283.64777,"DeltaM..ppm.":0.88,"Deltam.z..Da.":0.00057,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2367042,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":2.24234,"Ion.Inject.Time..ms.":22,"RT..min.":7.6425,"First.Scan":5437,"Last.Scan":5437,"Master.Scan.s.":5430,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_05.raw","File.ID":"F11","Quan.Info":"","Peptides.Matched":551,"XCorr":2.37,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":0.0001,"Percolator.SVMScore":1.372,"Precursor.Area":2408209.75,"Apex.RT..min.":7.65,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Z4)","Search.ID":"Z","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSSPLHRLSPTGK","Annotated.Sequence":"[R].mSsPLHRLsPTGK.[S]","Modifications":"M1(Oxidation); S3(Phospho); S9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.4241,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":529.56466,"Contaminant":"False","Aligned.m.z..Da.":529.56468,"MH...Da.":1586.67944,"Theo..MH...Da.":1586.67977,"DeltaM..ppm.":-0.21,"Deltam.z..Da.":-0.00011,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2134492.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":12.35456,"Ion.Inject.Time..ms.":22,"RT..min.":7.7281,"First.Scan":5559,"Last.Scan":5559,"Master.Scan.s.":5547,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_05.raw","File.ID":"F11","Quan.Info":"","Peptides.Matched":1026,"XCorr":3.23,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":0.00035,"Percolator.SVMScore":1.14,"Precursor.Area":2289851.75,"Apex.RT..min.":7.73,"ptmRS..Binomial.Peptide.Score":222.21,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(8): 877.50; y2+-H(3) O(4) P(10): 544.32; y2+-H(3) O(4) P(8): 439.25; y1+(4): 402.23; y1+(3): 305.18","ptmRS..Best.Site.Probabilities":"S3(Phospho): 100; S9(Phospho): 99.99","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(3): 100.0; S(9): 100.0; T(11): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Z4)","Search.ID":"Z","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DIFSNDELLSDAYDAK","Annotated.Sequence":"[K].DIFSNDELLSDAYDAK.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.522,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":605.94737,"Contaminant":"False","Aligned.m.z..Da.":605.94717,"MH...Da.":1815.82757,"Theo..MH...Da.":1815.82831,"DeltaM..ppm.":-0.41,"Deltam.z..Da.":-0.00025,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":259263.95312,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":17.1022,"First.Scan":17256,"Last.Scan":17256,"Master.Scan.s.":17248,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_05.raw","File.ID":"F11","Quan.Info":"","Peptides.Matched":1632,"XCorr":1.82,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":0.00036,"Percolator.SVMScore":1.135,"Precursor.Area":173764.5,"Apex.RT..min.":17.11,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Z4)","Search.ID":"Z","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DIFSNDELLSDAYDAK","Annotated.Sequence":"[K].DIFSNDELLSDAYDAK.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7805,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":908.41651,"Contaminant":"False","Aligned.m.z..Da.":908.4162,"MH...Da.":1815.82574,"Theo..MH...Da.":1815.82831,"DeltaM..ppm.":-1.42,"Deltam.z..Da.":-0.00129,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":472304.21875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":25.0777,"Ion.Inject.Time..ms.":22,"RT..min.":17.0876,"First.Scan":17240,"Last.Scan":17240,"Master.Scan.s.":17238,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_05.raw","File.ID":"F11","Quan.Info":"","Peptides.Matched":1568,"XCorr":4.01,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":1.169e-10,"Percolator.SVMScore":3.948,"Precursor.Area":717064.0625,"Apex.RT..min.":17.1,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Z4)","Search.ID":"Z","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEySDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); Y12(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":4,"DeltaScore":0.3481,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40611,"Contaminant":"False","Aligned.m.z..Da.":967.40624,"MH...Da.":2900.20377,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":1.53,"Deltam.z..Da.":0.00148,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":600379.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":90.71706,"Ion.Inject.Time..ms.":22,"RT..min.":15.2203,"First.Scan":15006,"Last.Scan":15006,"Master.Scan.s.":15005,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_05.raw","File.ID":"F11","Quan.Info":"","Peptides.Matched":2579,"XCorr":2.93,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":9.343e-06,"Percolator.SVMScore":1.826,"Precursor.Area":11190498,"Apex.RT..min.":15.09,"ptmRS..Binomial.Peptide.Score":186.33,"ptmRS..Isoform.Confidence.Probability":0.014,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(14): 1621.73","ptmRS..Best.Site.Probabilities":"S13(Phospho): 98.58","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 1.4; S(13): 98.6; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Z4)","Search.ID":"Z","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0497,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40325,"Contaminant":"False","Aligned.m.z..Da.":967.40336,"MH...Da.":2900.19521,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":-1.42,"Deltam.z..Da.":-0.00137,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1387990.875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":57.75466,"Ion.Inject.Time..ms.":22,"RT..min.":15.3647,"First.Scan":15189,"Last.Scan":15189,"Master.Scan.s.":15186,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_05.raw","File.ID":"F11","Quan.Info":"","Peptides.Matched":2256,"XCorr":3.42,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":2.765e-08,"Percolator.SVMScore":2.92,"Precursor.Area":1715486.75,"Apex.RT..min.":15.37,"ptmRS..Binomial.Peptide.Score":302.11,"ptmRS..Isoform.Confidence.Probability":0.5,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"Y12(Phospho): 50; S13(Phospho): 50","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 50.0; S(13): 50.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Z4)","Search.ID":"Z","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"GVFGYGFEEPSAIQQR","Annotated.Sequence":"[R].GVFGYGFEEPSAIQQR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6969,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":892.93272,"Contaminant":"False","Aligned.m.z..Da.":892.93285,"MH...Da.":1784.85817,"Theo..MH...Da.":1784.86022,"DeltaM..ppm.":-1.15,"Deltam.z..Da.":-0.00103,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":299913.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":40.03061,"Ion.Inject.Time..ms.":22,"RT..min.":15.0375,"First.Scan":14782,"Last.Scan":14782,"Master.Scan.s.":14777,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_05.raw","File.ID":"F11","Quan.Info":"","Peptides.Matched":1405,"XCorr":3.2,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":3.03e-09,"Percolator.SVMScore":3.336,"Precursor.Area":296959.625,"Apex.RT..min.":15.04,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Z4)","Search.ID":"Z","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FTVSAIYSDLPQQER","Annotated.Sequence":"[K].FTVSAIYSDLPQQER.[D]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6738,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":877.44232,"Contaminant":"False","Aligned.m.z..Da.":877.44245,"MH...Da.":1753.87737,"Theo..MH...Da.":1753.87554,"DeltaM..ppm.":1.04,"Deltam.z..Da.":0.00091,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":355948.21875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":15.0388,"First.Scan":14784,"Last.Scan":14784,"Master.Scan.s.":14777,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_05.raw","File.ID":"F11","Quan.Info":"","Peptides.Matched":1070,"XCorr":2.82,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":8.537e-07,"Percolator.SVMScore":2.275,"Precursor.Area":230696.01562,"Apex.RT..min.":15.04,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Z4)","Search.ID":"Z","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.068,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.4045,"Contaminant":"False","Aligned.m.z..Da.":967.40465,"MH...Da.":2900.19897,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":-0.12,"Deltam.z..Da.":-0.00012,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":456316,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":5.13673,"Ion.Inject.Time..ms.":22,"RT..min.":15.0478,"First.Scan":14795,"Last.Scan":14795,"Master.Scan.s.":14790,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_05.raw","File.ID":"F11","Quan.Info":"","Peptides.Matched":2497,"XCorr":5.44,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":1.014e-10,"Percolator.SVMScore":3.975,"Precursor.Area":11190498,"Apex.RT..min.":15.09,"ptmRS..Binomial.Peptide.Score":430.33,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(14): 1528.61; y1+-H(3) O(4) P(14): 1621.73; y2+-H(3) O(4) P(14): 811.37; b1+(12): 1344.56","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Z4)","Search.ID":"Z","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":5,"DeltaScore":0.0684,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40427,"Contaminant":"False","Aligned.m.z..Da.":967.40441,"MH...Da.":2900.19825,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":-0.37,"Deltam.z..Da.":-0.00036,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3174047.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":14.8002,"Ion.Inject.Time..ms.":22,"RT..min.":15.151,"First.Scan":14925,"Last.Scan":14925,"Master.Scan.s.":14920,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_05.raw","File.ID":"F11","Quan.Info":"","Peptides.Matched":2452,"XCorr":6.73,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":1.815e-15,"Percolator.SVMScore":6.035,"Precursor.Area":11190498,"Apex.RT..min.":15.09,"ptmRS..Binomial.Peptide.Score":630.61,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(13): 1413.58; b1+-H(3) O(4) P(14): 1528.61; b1+-H(3) O(4) P(15): 1642.65; y1+-H(3) O(4) P(15): 1750.78; y1+-H(3) O(4) P(14): 1621.73; y1+-H(3) O(4) P(13): 1458.67; b2+-H(3) O(4) P(16): 886.35; b2+-H(3) O(4) P(18): 1014.90; y2+-H(3) O(4) P(14): 811.37; y2+-H(3) O(4) P(13): 729.84; y1+(11): 1274.62; y1+(10): 1160.58; y1+(9): 1031.54; y1+(8): 903.48; y1+(7): 774.44; y1+(6): 661.35; y1+(5): 533.29; y1+(4): 420.21; y1+(3): 305.18; y1+(2): 234.14","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Z4)","Search.ID":"Z","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].msEGITDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S2(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1487,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1096.47186,"Contaminant":"False","Aligned.m.z..Da.":1096.47186,"MH...Da.":2191.93646,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":3.93,"Deltam.z..Da.":0.00431,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":226255.32812,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":37.21911,"Ion.Inject.Time..ms.":22,"RT..min.":15.7392,"First.Scan":15644,"Last.Scan":15644,"Master.Scan.s.":15632,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_05.raw","File.ID":"F11","Quan.Info":"","Peptides.Matched":2192,"XCorr":4.37,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":3.732e-10,"Percolator.SVMScore":3.73,"Precursor.Area":103198.83594,"Apex.RT..min.":15.53,"ptmRS..Binomial.Peptide.Score":523.04,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(2): 112.04; b1+-H(3) O(4) P(3): 241.08; b1+-H(3) O(4) P(4): 298.10; b1+-H(3) O(4) P(5): 411.19; b1+-H(3) O(4) P(6): 512.24; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+-H(3) O(4) P(9): 869.39; b1+-H(3) O(4) P(10): 998.43; b1+-H(3) O(4) P(11): 1085.46; b1+(4): 396.08; y1+(14): 1683.77","ptmRS..Best.Site.Probabilities":"S2(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 100.0; T(6): 0.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Z4)","Search.ID":"Z","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].msEGITDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S2(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0678,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":731.31338,"Contaminant":"False","Aligned.m.z..Da.":731.31337,"MH...Da.":2191.92559,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":-1.03,"Deltam.z..Da.":-0.00075,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":237381.76562,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":5.80776,"Ion.Inject.Time..ms.":22,"RT..min.":15.7516,"First.Scan":15657,"Last.Scan":15657,"Master.Scan.s.":15655,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_05.raw","File.ID":"F11","Quan.Info":"","Peptides.Matched":1883,"XCorr":3.54,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":1.216e-06,"Percolator.SVMScore":2.209,"Precursor.Area":1125566.875,"Apex.RT..min.":15.79,"ptmRS..Binomial.Peptide.Score":336.38,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(2): 112.04; b1+-H(3) O(4) P(3): 241.08; b1+-H(3) O(4) P(4): 298.10; b1+-H(3) O(4) P(5): 411.19; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+-H(3) O(4) P(9): 869.39","ptmRS..Best.Site.Probabilities":"S2(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 100.0; T(6): 0.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Z4)","Search.ID":"Z","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0548,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40834,"Contaminant":"False","Aligned.m.z..Da.":967.40841,"MH...Da.":2900.21049,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":3.85,"Deltam.z..Da.":0.00372,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":269951.84375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":11.18721,"Ion.Inject.Time..ms.":22,"RT..min.":15.555,"First.Scan":15427,"Last.Scan":15427,"Master.Scan.s.":15426,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_05.raw","File.ID":"F11","Quan.Info":"","Peptides.Matched":2647,"XCorr":4.56,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":4.309e-09,"Percolator.SVMScore":3.27,"ptmRS..Binomial.Peptide.Score":337.75,"ptmRS..Isoform.Confidence.Probability":0.992,"ptmRS..Isoform.Group.Report":"b1+(12): 1344.56","ptmRS..Best.Site.Probabilities":"S13(Phospho): 99.21","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.8; S(13): 99.2; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Z4)","Search.ID":"Z","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":4,"DeltaScore":0.0027,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40638,"Contaminant":"False","Aligned.m.z..Da.":967.40646,"MH...Da.":2900.20459,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":1.82,"Deltam.z..Da.":0.00176,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":295332.96875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":76.21101,"Ion.Inject.Time..ms.":22,"RT..min.":15.4893,"First.Scan":15346,"Last.Scan":15346,"Master.Scan.s.":15339,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_05.raw","File.ID":"F11","Quan.Info":"","Peptides.Matched":2546,"XCorr":3.71,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":6.569e-08,"Percolator.SVMScore":2.758,"Precursor.Area":11190498,"Apex.RT..min.":15.09,"ptmRS..Binomial.Peptide.Score":259.85,"ptmRS..Isoform.Confidence.Probability":0.5,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"Y12(Phospho): 50; S13(Phospho): 50","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 50.0; S(13): 50.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Z4)","Search.ID":"Z","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MFILDEADEMLSSGFK","Annotated.Sequence":"[K].mFILDEADEmLSSGFK.[E]","Modifications":"M1(Oxidation); M10(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.59,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":932.92364,"Contaminant":"False","Aligned.m.z..Da.":932.92328,"MH...Da.":1864.84001,"Theo..MH...Da.":1864.83433,"DeltaM..ppm.":3.05,"Deltam.z..Da.":0.00284,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":145873.17188,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":33.74473,"Ion.Inject.Time..ms.":22,"RT..min.":16.726,"First.Scan":16832,"Last.Scan":16832,"Master.Scan.s.":16830,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_05.raw","File.ID":"F11","Quan.Info":"","Peptides.Matched":1862,"XCorr":2,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":0.00035,"Percolator.SVMScore":1.139,"Precursor.Area":194612.35938,"Apex.RT..min.":16.78,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Z4)","Search.ID":"Z","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"AAEAGETGAATSATEGDNNNNTAAGDKK","Annotated.Sequence":"[K].AAEAGETGAATsATEGDNNNNTAAGDKK.[G]","Modifications":"S12(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0286,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":906.04889,"Contaminant":"False","Aligned.m.z..Da.":906.04897,"MH...Da.":2716.13213,"Theo..MH...Da.":2716.13298,"DeltaM..ppm.":-0.31,"Deltam.z..Da.":-0.00028,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3021728.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":3.85152,"Ion.Inject.Time..ms.":22,"RT..min.":5.0309,"First.Scan":1786,"Last.Scan":1786,"Master.Scan.s.":1777,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_05.raw","File.ID":"F11","Quan.Info":"","Peptides.Matched":2614,"XCorr":5.95,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":7.808e-15,"Percolator.SVMScore":5.756,"ptmRS..Binomial.Peptide.Score":422.28,"ptmRS..Isoform.Confidence.Probability":0.992,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(15): 1300.57; y1+-H(3) O(4) P(17): 1688.75; y2+-H(3) O(4) P(18): 895.40; y2+-H(3) O(4) P(17): 844.88","ptmRS..Best.Site.Probabilities":"S12(Phospho): 99.23","ptmRS..Phospho.Site.Probabilities":"T(7): 0.8; T(11): 0.0; S(12): 99.2; T(14): 0.0; T(22): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Z4)","Search.ID":"Z","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"TGTFSIAALQR","Annotated.Sequence":"[K].TGTFSIAALQR.[I]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7687,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":582.82225,"Contaminant":"False","Aligned.m.z..Da.":582.82224,"MH...Da.":1164.63723,"Theo..MH...Da.":1164.63715,"DeltaM..ppm.":0.07,"Deltam.z..Da.":0.00004,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":502975.28125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":14.10932,"Ion.Inject.Time..ms.":22,"RT..min.":13.375,"First.Scan":12826,"Last.Scan":12826,"Master.Scan.s.":12817,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_05.raw","File.ID":"F11","Quan.Info":"","Peptides.Matched":361,"XCorr":2.94,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":6.365e-06,"Percolator.SVMScore":1.898,"Precursor.Area":589389.625,"Apex.RT..min.":13.38,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Z4)","Search.ID":"Z","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"AIMPIIEGHDVLAQAQSGTGK","Annotated.Sequence":"[R].AImPIIEGHDVLAQAQSGTGK.[T]","Modifications":"M3(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.6119,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":718.04138,"Contaminant":"False","Aligned.m.z..Da.":718.04136,"MH...Da.":2152.10958,"Theo..MH...Da.":2152.10668,"DeltaM..ppm.":1.35,"Deltam.z..Da.":0.00097,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":401119.6875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":24.32026,"Ion.Inject.Time..ms.":22,"RT..min.":12.8211,"First.Scan":12123,"Last.Scan":12123,"Master.Scan.s.":12112,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_05.raw","File.ID":"F11","Quan.Info":"","Peptides.Matched":1000,"XCorr":2.68,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":4.28e-10,"Percolator.SVMScore":3.704,"Precursor.Area":497383.71875,"Apex.RT..min.":12.8,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Z4)","Search.ID":"Z","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MGSINNNFNTNNNSNTDLDRDWK","Annotated.Sequence":"[-].mGSINNNFNTNNNsNTDLDRDWK.[T]","Modifications":"N-Term(Prot)(Met-loss); S14(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P39517","Master.Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","Protein.Accessions":"P39517","Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0961,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":878.36973,"Contaminant":"False","Aligned.m.z..Da.":878.36972,"MH...Da.":2633.09463,"Theo..MH...Da.":2633.10123,"DeltaM..ppm.":-2.5,"Deltam.z..Da.":-0.0022,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":793573.875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":9.75338,"Ion.Inject.Time..ms.":22,"RT..min.":11.3417,"First.Scan":10101,"Last.Scan":10101,"Master.Scan.s.":10097,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_05.raw","File.ID":"F11","Quan.Info":"","Peptides.Matched":2118,"XCorr":3.85,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":1.214e-10,"Percolator.SVMScore":3.941,"Precursor.Area":815925.375,"Apex.RT..min.":11.35,"ptmRS..Binomial.Peptide.Score":446.4,"ptmRS..Isoform.Confidence.Probability":0.994,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(11): 1345.61; y1+-H(3) O(4) P(10): 1231.57; y2+-H(3) O(4) P(17): 1025.45; y2+-H(3) O(4) P(16): 968.43; y2+-H(3) O(4) P(15): 894.90; y2+-H(3) O(4) P(14): 837.88; y2+-H(3) O(4) P(13): 787.35; y2+-H(3) O(4) P(12): 730.33; y2+-H(3) O(4) P(11): 673.31; y2+-H(3) O(4) P(10): 616.29; y1+(9): 1162.55","ptmRS..Best.Site.Probabilities":"S14(Phospho): 99.4","ptmRS..Phospho.Site.Probabilities":"S(3): 0.0; T(10): 0.0; S(14): 99.4; T(16): 0.6"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Z4)","Search.ID":"Z","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MGSINNNFNTNNNSNTDLDRDWK","Annotated.Sequence":"[-].mGSINNNFNTNNNsNTDLDRDWK.[T]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S14(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P39517","Master.Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","Protein.Accessions":"P39517","Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.076,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":892.37898,"Contaminant":"False","Aligned.m.z..Da.":892.37897,"MH...Da.":2675.12241,"Theo..MH...Da.":2675.11179,"DeltaM..ppm.":3.97,"Deltam.z..Da.":0.00354,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":213351.98438,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":16.78086,"Ion.Inject.Time..ms.":22,"RT..min.":13.5484,"First.Scan":13043,"Last.Scan":13043,"Master.Scan.s.":13038,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_05.raw","File.ID":"F11","Quan.Info":"","Peptides.Matched":2439,"XCorr":4.34,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":2.266e-08,"Percolator.SVMScore":2.958,"ptmRS..Binomial.Peptide.Score":387.37,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(12): 1459.66; y1+-H(3) O(4) P(10): 1231.57; b2+-H(3) O(4) P(21): 1122.98; y2+-H(3) O(4) P(16): 968.43; y2+-H(3) O(4) P(15): 894.90; y2+-H(3) O(4) P(14): 837.88; y2+-H(3) O(4) P(13): 787.35; y1+(9): 1162.55; y1+(8): 1048.51","ptmRS..Best.Site.Probabilities":"S14(Phospho): 99.99","ptmRS..Phospho.Site.Probabilities":"S(3): 0.0; T(10): 0.0; S(14): 100.0; T(16): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Z4)","Search.ID":"Z","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MGSINNNFNTNNNSNTDLDRDWK","Annotated.Sequence":"[-].mGSINNNFNTNNNSNtDLDRDWK.[T]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); T16(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P39517","Master.Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","Protein.Accessions":"P39517","Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":4,"DeltaScore":0.0789,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":892.37898,"Contaminant":"False","Aligned.m.z..Da.":892.37897,"MH...Da.":2675.1224,"Theo..MH...Da.":2675.11179,"DeltaM..ppm.":3.97,"Deltam.z..Da.":0.00354,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":213351.98438,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":84.04833,"Ion.Inject.Time..ms.":22,"RT..min.":13.5523,"First.Scan":13049,"Last.Scan":13049,"Master.Scan.s.":13038,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_05.raw","File.ID":"F11","Quan.Info":"","Peptides.Matched":2439,"XCorr":3.04,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":0.00008,"Percolator.SVMScore":1.426,"ptmRS..Binomial.Peptide.Score":294.12,"ptmRS..Isoform.Confidence.Probability":0.5,"ptmRS..Isoform.Group.Report":"b2+-H(3) O(4) P(21): 1122.98; y2+-H(3) O(4) P(19): 1139.50; y2+-H(3) O(4) P(18): 1082.48; y2+-H(3) O(4) P(17): 1025.45; y2+-H(3) O(4) P(16): 968.43; y2+-H(3) O(4) P(15): 894.90; y2+-H(3) O(4) P(14): 837.88; y2+-H(3) O(4) P(13): 787.35","ptmRS..Best.Site.Probabilities":"S14(Phospho): 50; T16(Phospho): 50","ptmRS..Phospho.Site.Probabilities":"S(3): 0.0; T(10): 0.0; S(14): 50.0; T(16): 50.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"NHQLEEEIEK","Annotated.Sequence":"[K].NHQLEEEIEK.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.3789,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":634.80939,"Contaminant":"False","Aligned.m.z..Da.":634.8094,"MH...Da.":1268.61151,"Theo..MH...Da.":1268.61172,"DeltaM..ppm.":-0.16,"Deltam.z..Da.":-0.0001,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2669086,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":42.10603,"Ion.Inject.Time..ms.":22,"RT..min.":7.2014,"First.Scan":4672,"Last.Scan":4672,"Master.Scan.s.":4661,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":894,"XCorr":2.27,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":0.00005,"Percolator.SVMScore":1.869,"Precursor.Area":1929592.875,"Apex.RT..min.":7.2,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"RKVEELTTK","Annotated.Sequence":"[R].RKVEELTTK.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":2,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.5439,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":368.55225,"Contaminant":"False","Aligned.m.z..Da.":368.55227,"MH...Da.":1103.64221,"Theo..MH...Da.":1103.6419,"DeltaM..ppm.":0.28,"Deltam.z..Da.":0.0001,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2843002.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":55.75672,"Ion.Inject.Time..ms.":18.015,"RT..min.":5.032,"First.Scan":2278,"Last.Scan":2278,"Master.Scan.s.":2269,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":190,"XCorr":2.85,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":0.00003,"Percolator.SVMScore":1.987,"Precursor.Area":2903047.75,"Apex.RT..min.":5.04,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"AAEAGETGAATSATEGDNNNNTAAGDK","Annotated.Sequence":"[K].AAEAGETGAATSATEGDNNNNTAAGDK.[K]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.7511,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":836.69494,"Contaminant":"False","Aligned.m.z..Da.":836.69498,"MH...Da.":2508.07027,"Theo..MH...Da.":2508.07169,"DeltaM..ppm.":-0.56,"Deltam.z..Da.":-0.00047,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3558530,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":2.06888,"Ion.Inject.Time..ms.":22,"RT..min.":5.5242,"First.Scan":2897,"Last.Scan":2897,"Master.Scan.s.":2893,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":2759,"XCorr":7.11,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":2.014e-11,"Percolator.SVMScore":5.464,"Precursor.Area":2220573.25,"Apex.RT..min.":5.52,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"AAEAGETGAATSATEGDNNNNTAAGDKK","Annotated.Sequence":"[K].AAEAGETGAATsATEGDNNNNTAAGDKK.[G]","Modifications":"S12(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0211,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":906.04916,"Contaminant":"False","Aligned.m.z..Da.":906.04919,"MH...Da.":2716.13292,"Theo..MH...Da.":2716.13298,"DeltaM..ppm.":-0.02,"Deltam.z..Da.":-0.00002,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1202969.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":3.10224,"Ion.Inject.Time..ms.":22,"RT..min.":4.9371,"First.Scan":2143,"Last.Scan":2143,"Master.Scan.s.":2139,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":2538,"XCorr":7.11,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":4.559e-15,"Percolator.SVMScore":7.507,"Precursor.Area":9098727,"Apex.RT..min.":4.95,"ptmRS..Binomial.Peptide.Score":486.89,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(15): 1300.57; y1+-H(3) O(4) P(18): 1789.79; y2+-H(3) O(4) P(22): 1045.47; y2+-H(3) O(4) P(21): 994.95; y2+-H(3) O(4) P(18): 895.40; y2+-H(3) O(4) P(17): 844.88; y1+(17): 1786.72","ptmRS..Best.Site.Probabilities":"S12(Phospho): 99.99","ptmRS..Phospho.Site.Probabilities":"T(7): 0.0; T(11): 0.0; S(12): 100.0; T(14): 0.0; T(22): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"AAEAGETGAATSATEGDNNNNTAAGDKK","Annotated.Sequence":"[K].AAEAGETGAATSATEGDNNNNTAAGDKK.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.7604,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":879.39439,"Contaminant":"False","Aligned.m.z..Da.":879.39442,"MH...Da.":2636.16863,"Theo..MH...Da.":2636.16665,"DeltaM..ppm.":0.75,"Deltam.z..Da.":0.00066,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":4185832.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":5.48873,"Ion.Inject.Time..ms.":22,"RT..min.":4.902,"First.Scan":2092,"Last.Scan":2092,"Master.Scan.s.":2087,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":3467,"XCorr":8.43,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":1.625e-17,"Percolator.SVMScore":8.88,"Precursor.Area":4100829,"Apex.RT..min.":4.91,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KFQDDTLNR","Annotated.Sequence":"[K].KFQDDtLNR.[V]","Modifications":"T6(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7096,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":608.7713,"Contaminant":"False","Aligned.m.z..Da.":608.77132,"MH...Da.":1216.53533,"Theo..MH...Da.":1216.53579,"DeltaM..ppm.":-0.38,"Deltam.z..Da.":-0.00023,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":4987034.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":21.28618,"Ion.Inject.Time..ms.":22,"RT..min.":6.3587,"First.Scan":3746,"Last.Scan":3746,"Master.Scan.s.":3738,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":610,"XCorr":3.34,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":0.0004,"Percolator.SVMScore":1.366,"Precursor.Area":4093185,"Apex.RT..min.":6.36,"ptmRS..Binomial.Peptide.Score":376.61,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"T6(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"T(6): 100.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"AIMPIIEGHDVLAQAQSGTGK","Annotated.Sequence":"[R].AImPIIEGHDVLAQAQSGTGK.[T]","Modifications":"M3(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.7143,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":718.03923,"Contaminant":"False","Aligned.m.z..Da.":718.03921,"MH...Da.":2152.10315,"Theo..MH...Da.":2152.10668,"DeltaM..ppm.":-1.64,"Deltam.z..Da.":-0.00117,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1699767.125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":23.12437,"Ion.Inject.Time..ms.":22,"RT..min.":12.7229,"First.Scan":11988,"Last.Scan":11988,"Master.Scan.s.":11980,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":1030,"XCorr":4.97,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":2.619e-10,"Percolator.SVMScore":4.84,"Precursor.Area":3469824.75,"Apex.RT..min.":12.75,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"TGTFSIAALQR","Annotated.Sequence":"[K].TGTFSIAALQR.[I]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7659,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":582.82281,"Contaminant":"False","Aligned.m.z..Da.":582.82288,"MH...Da.":1164.63835,"Theo..MH...Da.":1164.63715,"DeltaM..ppm.":1.03,"Deltam.z..Da.":0.0006,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":936931.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":38.02224,"Ion.Inject.Time..ms.":22,"RT..min.":13.3653,"First.Scan":12820,"Last.Scan":12820,"Master.Scan.s.":12812,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":360,"XCorr":3.46,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":9.122e-06,"Percolator.SVMScore":2.294,"Precursor.Area":5224369,"Apex.RT..min.":13.39,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"GVAINFVTNEDVGAMRELEK","Annotated.Sequence":"[K].GVAINFVTNEDVGAmRELEK.[F]","Modifications":"M15(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.5437,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":736.70412,"Contaminant":"False","Aligned.m.z..Da.":736.70421,"MH...Da.":2208.09781,"Theo..MH...Da.":2208.09651,"DeltaM..ppm.":0.59,"Deltam.z..Da.":0.00043,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":601399.0625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":4.67196,"Ion.Inject.Time..ms.":22,"RT..min.":13.4148,"First.Scan":12885,"Last.Scan":12885,"Master.Scan.s.":12877,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":1460,"XCorr":2.63,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":6.863e-06,"Percolator.SVMScore":2.363,"Precursor.Area":443228.71875,"Apex.RT..min.":13.4,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"MGSINNNFNTNNNSNTDLDRDWK","Annotated.Sequence":"[-].mGSINNNFNTNNNsNTDLDRDWK.[T]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S14(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P39517","Master.Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","Protein.Accessions":"P39517","Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.0194,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1338.06012,"Contaminant":"False","Aligned.m.z..Da.":1338.06033,"MH...Da.":2675.11297,"Theo..MH...Da.":2675.11179,"DeltaM..ppm.":0.44,"Deltam.z..Da.":0.00059,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1196899.125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":81.44495,"Ion.Inject.Time..ms.":22,"RT..min.":13.532,"First.Scan":13038,"Last.Scan":13038,"Master.Scan.s.":13033,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":2235,"XCorr":4.13,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":5.864e-07,"Percolator.SVMScore":2.962,"ptmRS..Binomial.Peptide.Score":474.61,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(15): 1788.79; y1+-H(3) O(4) P(14): 1674.75; y1+-H(3) O(4) P(13): 1573.70; y1+-H(3) O(4) P(12): 1459.66; y1+-H(3) O(4) P(10): 1231.57; b2+-H(3) O(4) P(19): 987.41; y2+-H(3) O(4) P(19): 1139.50; y1+(9): 1162.55; y1+(8): 1048.51","ptmRS..Best.Site.Probabilities":"S14(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(3): 0.0; T(10): 0.0; S(14): 100.0; T(16): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"VHACIGGTSFVEDAEGLR","Annotated.Sequence":"[K].VHAcIGGTSFVEDAEGLR.[D]","Modifications":"C4(Carbamidomethyl)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.5345,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":639.97392,"Contaminant":"False","Aligned.m.z..Da.":639.97395,"MH...Da.":1917.90721,"Theo..MH...Da.":1917.91234,"DeltaM..ppm.":-2.67,"Deltam.z..Da.":-0.00171,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1358960.375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":68.8289,"Ion.Inject.Time..ms.":22,"RT..min.":13.1381,"First.Scan":12523,"Last.Scan":12523,"Master.Scan.s.":12513,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":1633,"XCorr":2.75,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":0.00002,"Percolator.SVMScore":2.166,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DFLLKPELSR","Annotated.Sequence":"[K].DFLLKPELSR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.6167,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":406.56792,"Contaminant":"False","Aligned.m.z..Da.":406.56795,"MH...Da.":1217.68922,"Theo..MH...Da.":1217.68885,"DeltaM..ppm.":0.3,"Deltam.z..Da.":0.00012,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1501191.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":5.90252,"Ion.Inject.Time..ms.":22,"RT..min.":13.1637,"First.Scan":12555,"Last.Scan":12555,"Master.Scan.s.":12552,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":265,"XCorr":2.4,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":0.00028,"Percolator.SVMScore":1.451,"Precursor.Area":1794193.5,"Apex.RT..min.":13.18,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"APQALMLAPTR","Annotated.Sequence":"[K].APQALMLAPTR.[E]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.674,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":584.82802,"Contaminant":"False","Aligned.m.z..Da.":584.82803,"MH...Da.":1168.64876,"Theo..MH...Da.":1168.65069,"DeltaM..ppm.":-1.65,"Deltam.z..Da.":-0.00097,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3412848.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":55.6286,"Ion.Inject.Time..ms.":22,"RT..min.":11.6279,"First.Scan":10631,"Last.Scan":10631,"Master.Scan.s.":10628,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":244,"XCorr":3.62,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":1.131e-06,"Percolator.SVMScore":2.802,"Precursor.Area":3082979.5,"Apex.RT..min.":11.64,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"MGSINNNFNTNNNSNTDLDRDWK","Annotated.Sequence":"[-].mGSINNNFNTNnNsNTDLDRDWK.[T]","Modifications":"N-Term(Prot)(Met-loss); N12(Deamidated); S14(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P39517","Master.Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","Protein.Accessions":"P39517","Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0161,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":878.69988,"Contaminant":"False","Aligned.m.z..Da.":878.69991,"MH...Da.":2634.08508,"Theo..MH...Da.":2634.08524,"DeltaM..ppm.":-0.06,"Deltam.z..Da.":-0.00005,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1351845.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":8.44545,"Ion.Inject.Time..ms.":22,"RT..min.":11.6122,"First.Scan":10613,"Last.Scan":10613,"Master.Scan.s.":10602,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":1950,"XCorr":4.34,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":1.143e-08,"Percolator.SVMScore":3.92,"Precursor.Area":1254787.125,"Apex.RT..min.":11.61,"ptmRS..Binomial.Peptide.Score":447,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(13): 1574.68; y1+-H(3) O(4) P(12): 1460.64; y1+-H(3) O(4) P(11): 1345.61; y1+-H(3) O(4) P(10): 1231.57; y2+-H(3) O(4) P(19): 1139.99; y2+-H(3) O(4) P(18): 1082.97; y2+-H(3) O(4) P(17): 1025.95; y2+-H(3) O(4) P(16): 968.92; y2+-H(3) O(4) P(15): 895.39; y2+-H(3) O(4) P(14): 838.37; y2+-H(3) O(4) P(12): 730.82; y2+-H(3) O(4) P(11): 673.31; y1+(9): 1162.55; y1+(8): 1048.51","ptmRS..Best.Site.Probabilities":"S14(Phospho): 99.99","ptmRS..Phospho.Site.Probabilities":"S(3): 0.0; T(10): 0.0; S(14): 100.0; T(16): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"TAVFYGGTPISK","Annotated.Sequence":"[K].TAVFYGGTPISK.[D]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5115,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":620.83157,"Contaminant":"False","Aligned.m.z..Da.":620.83163,"MH...Da.":1240.65586,"Theo..MH...Da.":1240.65721,"DeltaM..ppm.":-1.09,"Deltam.z..Da.":-0.00068,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2259646,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":11.89544,"Ion.Inject.Time..ms.":22,"RT..min.":11.1516,"First.Scan":10031,"Last.Scan":10031,"Master.Scan.s.":10030,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":408,"XCorr":2.62,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":0.00002,"Percolator.SVMScore":2.107,"Precursor.Area":1708291.75,"Apex.RT..min.":11.15,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"SSQIHIENESTEDILK","Annotated.Sequence":"[R].SSQIHIENEStEDILK.[I]","Modifications":"T11(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0708,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":641.62968,"Contaminant":"False","Aligned.m.z..Da.":641.62973,"MH...Da.":1922.87448,"Theo..MH...Da.":1922.87429,"DeltaM..ppm.":0.1,"Deltam.z..Da.":0.00006,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1634255.375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":16.85206,"Ion.Inject.Time..ms.":22,"RT..min.":11.3099,"First.Scan":10234,"Last.Scan":10234,"Master.Scan.s.":10225,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":1813,"XCorr":4.52,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":0.00003,"Percolator.SVMScore":2.033,"Precursor.Area":1613360.125,"Apex.RT..min.":11.32,"ptmRS..Binomial.Peptide.Score":327.48,"ptmRS..Isoform.Confidence.Probability":0.5,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(10): 1159.55; y1+-H(3) O(4) P(9): 1030.51; y1+-H(3) O(4) P(7): 787.42; b2+-H(3) O(4) P(10): 554.26; y2+-H(3) O(4) P(7): 394.21","ptmRS..Best.Site.Probabilities":"S10(Phospho): 50; T11(Phospho): 50","ptmRS..Phospho.Site.Probabilities":"S(1): 0.0; S(2): 0.0; S(10): 50.0; T(11): 50.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"LTESTVNHTNHSDDELPGHLLLDSGASR","Annotated.Sequence":"[K].LTESTVNHtNHSDDELPGHLLLDSGASR.[T]","Modifications":"T9(Phospho)","X..Proteins":15,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"Q99337; P0C2I9; P47100; P0C2J0; Q03619; P0C2I2; Q99231; Q12088; Q07793; Q04711; Q12316; O13535; Q04670; Q92393; Q12193","Protein.Descriptions":"Transposon Ty1-NL2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-NL2 PE=3 SV=1; Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-ER2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER2 PE=3 SV=1; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-LR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR1 PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-GR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR3 PE=3 SV=3; Transposon Ty1-H Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-H PE=3 SV=1; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-BR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-BR PE=3 SV=1","X..Missed.Cleavages":0,"Charge":4,"Original.Precursor.Charge":4,"DeltaScore":0.0024,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":774.60649,"Contaminant":"False","Aligned.m.z..Da.":774.60645,"MH...Da.":3095.40415,"Theo..MH...Da.":3095.40658,"DeltaM..ppm.":-0.78,"Deltam.z..Da.":-0.00061,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":856051.5625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":8.82222,"Ion.Inject.Time..ms.":22,"RT..min.":12.2549,"First.Scan":11405,"Last.Scan":11405,"Master.Scan.s.":11395,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":4650,"XCorr":4.24,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":6.716e-07,"Percolator.SVMScore":2.929,"Precursor.Area":1217438.375,"Apex.RT..min.":12.26,"ptmRS..Binomial.Peptide.Score":252.57,"ptmRS..Isoform.Confidence.Probability":0.012,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(2): 197.13; b1+-H(3) O(4) P(3): 326.17; b1+-H(3) O(4) P(6): 613.32; y2+-H(3) O(4) P(27): 1442.68","ptmRS..Best.Site.Probabilities":"T2(Phospho): 95.26","ptmRS..Phospho.Site.Probabilities":"T(2): 95.3; S(4): 1.2; T(5): 1.2; T(9): 1.2; S(12): 1.2; S(24): 0.0; S(27): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MGSINNNFNTNNNSNTDLDR","Annotated.Sequence":"[-].mGSINNNFNTNNNsNTDLDR.[D]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S14(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P39517","Master.Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","Protein.Accessions":"P39517","Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.1033,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":749.30954,"Contaminant":"False","Aligned.m.z..Da.":749.30952,"MH...Da.":2245.91406,"Theo..MH...Da.":2245.91057,"DeltaM..ppm.":1.55,"Deltam.z..Da.":0.00116,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":887091.9375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":13.62027,"Ion.Inject.Time..ms.":22,"RT..min.":11.9489,"First.Scan":11028,"Last.Scan":11028,"Master.Scan.s.":11018,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":1470,"XCorr":3.29,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":0.00003,"Percolator.SVMScore":1.969,"ptmRS..Binomial.Peptide.Score":277.22,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(12): 1359.59; y1+-H(3) O(4) P(11): 1245.55; y1+-H(3) O(4) P(10): 1144.50; y1+-H(3) O(4) P(9): 1030.45; y1+-H(3) O(4) P(8): 916.41; y1+-H(3) O(4) P(7): 802.37; y1+(6): 733.35; y1+(5): 619.30","ptmRS..Best.Site.Probabilities":"S14(Phospho): 99.99","ptmRS..Phospho.Site.Probabilities":"S(3): 0.0; T(10): 0.0; S(14): 100.0; T(16): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].mSEGItDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); T6(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1145,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1096.47156,"Contaminant":"False","Aligned.m.z..Da.":1096.47164,"MH...Da.":2191.93586,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":3.65,"Deltam.z..Da.":0.00401,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1588741,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":9.42474,"Ion.Inject.Time..ms.":22,"RT..min.":15.4727,"First.Scan":15543,"Last.Scan":15543,"Master.Scan.s.":15542,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":2140,"XCorr":6.29,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":2.149e-10,"Percolator.SVMScore":4.888,"Precursor.Area":1347091.5,"Apex.RT..min.":15.48,"ptmRS..Binomial.Peptide.Score":535.81,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(6): 512.24; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+-H(3) O(4) P(9): 869.39; b1+-H(3) O(4) P(10): 998.43; b1+-H(3) O(4) P(13): 1326.61; y1+-H(3) O(4) P(14): 1665.76; b1+(2): 130.05; b1+(3): 259.09; b1+(4): 316.11; b1+(5): 429.20; b2+(3): 130.05","ptmRS..Best.Site.Probabilities":"T6(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; T(6): 100.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].mSEGItDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); T6(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.1678,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":731.31474,"Contaminant":"False","Aligned.m.z..Da.":731.3148,"MH...Da.":2191.92968,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":0.84,"Deltam.z..Da.":0.00061,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":716387.875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":4.12397,"Ion.Inject.Time..ms.":22,"RT..min.":15.4554,"First.Scan":15522,"Last.Scan":15522,"Master.Scan.s.":15516,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":2033,"XCorr":4.35,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":1.122e-06,"Percolator.SVMScore":2.804,"Precursor.Area":1509385.5,"Apex.RT..min.":15.47,"ptmRS..Binomial.Peptide.Score":386.64,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(6): 512.24; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+-H(3) O(4) P(9): 869.39; b1+-H(3) O(4) P(10): 998.43; b1+(2): 130.05; b1+(3): 259.09; b1+(4): 316.11; b1+(5): 429.20; b2+(3): 130.05","ptmRS..Best.Site.Probabilities":"T6(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; T(6): 100.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].msEGITDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S2(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":3,"DeltaScore":0.1454,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1096.46787,"Contaminant":"False","Aligned.m.z..Da.":1096.4679,"MH...Da.":2191.92847,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":0.29,"Deltam.z..Da.":0.00031,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":5633250.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":20.03349,"Ion.Inject.Time..ms.":22,"RT..min.":15.6909,"First.Scan":15824,"Last.Scan":15824,"Master.Scan.s.":15815,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":1973,"XCorr":5.09,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":1.58e-09,"Percolator.SVMScore":4.402,"Precursor.Area":46381100,"Apex.RT..min.":15.72,"ptmRS..Binomial.Peptide.Score":586.39,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(3): 241.08; b1+-H(3) O(4) P(4): 298.10; b1+-H(3) O(4) P(5): 411.19; b1+-H(3) O(4) P(6): 512.24; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+-H(3) O(4) P(9): 869.39; b1+-H(3) O(4) P(10): 998.43; b1+-H(3) O(4) P(11): 1085.46; b1+-H(3) O(4) P(13): 1326.61; b1+(4): 396.08; b1+(5): 509.16; y1+(16): 1853.88; y1+(14): 1683.77","ptmRS..Best.Site.Probabilities":"S2(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 100.0; T(6): 0.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":4,"DeltaScore":0.0347,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40522,"Contaminant":"False","Aligned.m.z..Da.":967.40524,"MH...Da.":2900.20112,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":0.62,"Deltam.z..Da.":0.0006,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1555229.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":40.97543,"Ion.Inject.Time..ms.":22,"RT..min.":15.7589,"First.Scan":15910,"Last.Scan":15910,"Master.Scan.s.":15906,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":2512,"XCorr":4.61,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":5.719e-07,"Percolator.SVMScore":2.968,"Precursor.Area":1156306.5,"Apex.RT..min.":15.93,"ptmRS..Binomial.Peptide.Score":376.2,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(13): 1413.58; b1+-H(3) O(4) P(14): 1528.61; y1+-H(3) O(4) P(15): 1750.78; y2+-H(3) O(4) P(14): 811.37; b1+(12): 1344.56","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":5,"DeltaScore":0.0652,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40444,"Contaminant":"False","Aligned.m.z..Da.":967.40448,"MH...Da.":2900.19877,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":-0.19,"Deltam.z..Da.":-0.00019,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1487505.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":19.62082,"Ion.Inject.Time..ms.":22,"RT..min.":15.6361,"First.Scan":15752,"Last.Scan":15752,"Master.Scan.s.":15750,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":2478,"XCorr":5.37,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":1.567e-08,"Percolator.SVMScore":3.844,"Precursor.Area":2538903.25,"Apex.RT..min.":15.77,"ptmRS..Binomial.Peptide.Score":396.06,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(14): 1621.73; b2+-H(3) O(4) P(18): 1014.90; y2+-H(3) O(4) P(14): 811.37; b1+(12): 1344.56","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ILISTDLLAR","Annotated.Sequence":"[R].ILISTDLLAR.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5169,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":557.84548,"Contaminant":"False","Aligned.m.z..Da.":557.84551,"MH...Da.":1114.68369,"Theo..MH...Da.":1114.68304,"DeltaM..ppm.":0.59,"Deltam.z..Da.":0.00033,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3251780.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":20.79616,"Ion.Inject.Time..ms.":22,"RT..min.":15.6188,"First.Scan":15731,"Last.Scan":15731,"Master.Scan.s.":15724,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":124,"XCorr":2.67,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":0.00014,"Percolator.SVMScore":1.625,"Precursor.Area":3866460.25,"Apex.RT..min.":15.63,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ILISTDLLAR","Annotated.Sequence":"[R].ILISTDLLAR.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.4661,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":557.84548,"Contaminant":"False","Aligned.m.z..Da.":557.84551,"MH...Da.":1114.68369,"Theo..MH...Da.":1114.68304,"DeltaM..ppm.":0.59,"Deltam.z..Da.":0.00033,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1343707.125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":20.62787,"Ion.Inject.Time..ms.":22,"RT..min.":15.6061,"First.Scan":15714,"Last.Scan":15714,"Master.Scan.s.":15711,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":124,"XCorr":2.36,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":0.00027,"Percolator.SVMScore":1.468,"Precursor.Area":3866460.25,"Apex.RT..min.":15.63,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":5,"DeltaScore":0.0714,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40489,"Contaminant":"False","Aligned.m.z..Da.":967.40503,"MH...Da.":2900.20011,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":0.27,"Deltam.z..Da.":0.00026,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":16263560,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":16.09848,"Ion.Inject.Time..ms.":12.942,"RT..min.":15.125,"First.Scan":15103,"Last.Scan":15103,"Master.Scan.s.":15100,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":2493,"XCorr":7,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":1.22e-11,"Percolator.SVMScore":5.586,"Precursor.Area":90304056,"Apex.RT..min.":15.06,"ptmRS..Binomial.Peptide.Score":746.22,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(13): 1413.58; b1+-H(3) O(4) P(14): 1528.61; b1+-H(3) O(4) P(15): 1642.65; b1+-H(3) O(4) P(16): 1771.69; y1+-H(3) O(4) P(15): 1750.78; y1+-H(3) O(4) P(14): 1621.73; y1+-H(3) O(4) P(13): 1458.67; b2+-H(3) O(4) P(16): 886.35; b2+-H(3) O(4) P(17): 950.38; b2+-H(3) O(4) P(18): 1014.90; y2+-H(3) O(4) P(14): 811.37; b1+(12): 1344.56; y1+(13): 1556.65","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"GVFGYGFEEPSAIQQR","Annotated.Sequence":"[R].GVFGYGFEEPSAIQQR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6349,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":892.93357,"Contaminant":"False","Aligned.m.z..Da.":892.93372,"MH...Da.":1784.85987,"Theo..MH...Da.":1784.86022,"DeltaM..ppm.":-0.2,"Deltam.z..Da.":-0.00018,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":4851627.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":26.76875,"Ion.Inject.Time..ms.":22,"RT..min.":15.0319,"First.Scan":14984,"Last.Scan":14984,"Master.Scan.s.":14983,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":1263,"XCorr":3.15,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":2.276e-07,"Percolator.SVMScore":3.192,"Precursor.Area":3877823,"Apex.RT..min.":15.04,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"GVFGYGFEEPSAIQQR","Annotated.Sequence":"[R].GVFGYGFEEPSAIQQR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.6608,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":595.62558,"Contaminant":"False","Aligned.m.z..Da.":595.62568,"MH...Da.":1784.86218,"Theo..MH...Da.":1784.86022,"DeltaM..ppm.":1.1,"Deltam.z..Da.":0.00065,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":651535.0625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":27.05781,"Ion.Inject.Time..ms.":22,"RT..min.":15.0268,"First.Scan":14979,"Last.Scan":14979,"Master.Scan.s.":14970,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":1456,"XCorr":3.39,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":0.00002,"Percolator.SVMScore":2.092,"Precursor.Area":1170051.125,"Apex.RT..min.":15.04,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FFISNPDLVDRLEK","Annotated.Sequence":"[R].FFISNPDLVDRLEK.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q03558","Master.Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","Protein.Accessions":"Q03558","Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.7812,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":564.96957,"Contaminant":"False","Aligned.m.z..Da.":564.96962,"MH...Da.":1692.89416,"Theo..MH...Da.":1692.89555,"DeltaM..ppm.":-0.82,"Deltam.z..Da.":-0.00046,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":708403.9375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":33.32437,"Ion.Inject.Time..ms.":22,"RT..min.":15.3428,"First.Scan":15379,"Last.Scan":15379,"Master.Scan.s.":15373,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":530,"XCorr":3.2,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":7.696e-06,"Percolator.SVMScore":2.335,"Precursor.Area":893998.9375,"Apex.RT..min.":15.36,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0548,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40553,"Contaminant":"False","Aligned.m.z..Da.":967.40564,"MH...Da.":2900.20204,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":0.93,"Deltam.z..Da.":0.0009,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3841036.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":25.26872,"Ion.Inject.Time..ms.":22,"RT..min.":15.2887,"First.Scan":15309,"Last.Scan":15309,"Master.Scan.s.":15308,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":2543,"XCorr":6.2,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":2.87e-08,"Percolator.SVMScore":3.696,"Precursor.Area":90304056,"Apex.RT..min.":15.06,"ptmRS..Binomial.Peptide.Score":541.65,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(13): 1413.58; b1+-H(3) O(4) P(14): 1528.61; b1+-H(3) O(4) P(15): 1642.65; b1+-H(3) O(4) P(16): 1771.69; y1+-H(3) O(4) P(15): 1750.78; y1+-H(3) O(4) P(14): 1621.73; y1+-H(3) O(4) P(13): 1458.67; y2+-H(3) O(4) P(14): 811.37; b1+(12): 1344.56","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.4,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40899,"Contaminant":"False","Aligned.m.z..Da.":967.40895,"MH...Da.":2900.21243,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":4.52,"Deltam.z..Da.":0.00437,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":562385.5625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":55.49046,"Ion.Inject.Time..ms.":22,"RT..min.":16.2721,"First.Scan":16572,"Last.Scan":16572,"Master.Scan.s.":16569,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":2681,"XCorr":3.6,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":0.00004,"Percolator.SVMScore":1.914,"ptmRS..Binomial.Peptide.Score":310.49,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(14): 1621.73; b2+-H(3) O(4) P(24): 1328.56","ptmRS..Best.Site.Probabilities":"S13(Phospho): 99.99","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0638,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40607,"Contaminant":"False","Aligned.m.z..Da.":967.40603,"MH...Da.":2900.20367,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":1.5,"Deltam.z..Da.":0.00145,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":613214.375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":38.22723,"Ion.Inject.Time..ms.":22,"RT..min.":16.3008,"First.Scan":16609,"Last.Scan":16609,"Master.Scan.s.":16608,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":2599,"XCorr":3.92,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":3.266e-06,"Percolator.SVMScore":2.544,"Precursor.Area":794217,"Apex.RT..min.":16.25,"ptmRS..Binomial.Peptide.Score":300.97,"ptmRS..Isoform.Confidence.Probability":0.99,"ptmRS..Isoform.Group.Report":"b1+(12): 1344.56","ptmRS..Best.Site.Probabilities":"S13(Phospho): 98.98","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 1.0; S(13): 99.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].msEGITDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S2(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1397,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1096.46799,"Contaminant":"False","Aligned.m.z..Da.":1096.46798,"MH...Da.":2191.9287,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":0.39,"Deltam.z..Da.":0.00043,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1093871.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":43.88526,"Ion.Inject.Time..ms.":22,"RT..min.":15.9046,"First.Scan":16100,"Last.Scan":16100,"Master.Scan.s.":16088,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":1965,"XCorr":3.58,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":8.89e-07,"Percolator.SVMScore":2.861,"Precursor.Area":46381100,"Apex.RT..min.":15.72,"ptmRS..Binomial.Peptide.Score":326.29,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(2): 112.04; b1+-H(3) O(4) P(3): 241.08; b1+-H(3) O(4) P(4): 298.10; b1+-H(3) O(4) P(5): 411.19; b1+-H(3) O(4) P(6): 512.24; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+-H(3) O(4) P(9): 869.39","ptmRS..Best.Site.Probabilities":"S2(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 100.0; T(6): 0.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].msEGITDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S2(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1422,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1096.4658,"Contaminant":"False","Aligned.m.z..Da.":1096.46578,"MH...Da.":2191.92432,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":-1.61,"Deltam.z..Da.":-0.00176,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":536692.625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":15.9983,"First.Scan":16219,"Last.Scan":16219,"Master.Scan.s.":16218,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":1875,"XCorr":4.43,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":9.062e-09,"Percolator.SVMScore":3.977,"Precursor.Area":46381100,"Apex.RT..min.":15.72,"ptmRS..Binomial.Peptide.Score":500.81,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(2): 112.04; b1+-H(3) O(4) P(3): 241.08; b1+-H(3) O(4) P(4): 298.10; b1+-H(3) O(4) P(5): 411.19; b1+-H(3) O(4) P(6): 512.24; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+-H(3) O(4) P(9): 869.39; b1+-H(3) O(4) P(10): 998.43; b1+(4): 396.08; y1+(14): 1683.77","ptmRS..Best.Site.Probabilities":"S2(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 100.0; T(6): 0.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FDDMELDENLLR","Annotated.Sequence":"[K].FDDMELDENLLR.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5546,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":755.34907,"Contaminant":"False","Aligned.m.z..Da.":755.34905,"MH...Da.":1509.69087,"Theo..MH...Da.":1509.68898,"DeltaM..ppm.":1.25,"Deltam.z..Da.":0.00094,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":769825.625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":16.0301,"First.Scan":16261,"Last.Scan":16261,"Master.Scan.s.":16257,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":1017,"XCorr":3.48,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":1.777e-06,"Percolator.SVMScore":2.692,"Precursor.Area":1930651.75,"Apex.RT..min.":16.05,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.04,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40398,"Contaminant":"False","Aligned.m.z..Da.":967.40419,"MH...Da.":2900.19739,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":-0.67,"Deltam.z..Da.":-0.00065,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":702607.9375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":14.7772,"First.Scan":14661,"Last.Scan":14661,"Master.Scan.s.":14658,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":2405,"XCorr":5.25,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":3.468e-08,"Percolator.SVMScore":3.65,"Precursor.Area":2009959.125,"Apex.RT..min.":14.8,"ptmRS..Binomial.Peptide.Score":477.42,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(13): 1413.58; y1+-H(3) O(4) P(15): 1750.78; y1+-H(3) O(4) P(14): 1621.73; y1+-H(3) O(4) P(13): 1458.67; y2+-H(3) O(4) P(14): 811.37; b1+(12): 1344.56","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].mSEGITDIEEsQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S11(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1952,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1096.46728,"Contaminant":"False","Aligned.m.z..Da.":1096.46748,"MH...Da.":2191.92728,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":-0.26,"Deltam.z..Da.":-0.00028,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":538040.625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":14.9553,"First.Scan":14888,"Last.Scan":14888,"Master.Scan.s.":14879,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":1943,"XCorr":4.61,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":1.258e-07,"Percolator.SVMScore":3.337,"Precursor.Area":559477.625,"Apex.RT..min.":14.96,"ptmRS..Binomial.Peptide.Score":437.81,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(14): 1665.76; y1+-H(3) O(4) P(13): 1564.71; y1+-H(3) O(4) P(12): 1449.69; y1+-H(3) O(4) P(11): 1336.60; y1+-H(3) O(4) P(10): 1207.56; y1+-H(3) O(4) P(9): 1078.52; y2+-H(3) O(4) P(18): 1026.47; y1+(8): 1009.49; y1+(7): 881.44; y1+(6): 768.35; y1+(5): 640.29","ptmRS..Best.Site.Probabilities":"S11(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; T(6): 0.0; S(11): 100.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"GVFGYGFEEPSAIQQR","Annotated.Sequence":"[R].GVFGYGFEEPSAIQQR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6657,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":892.93516,"Contaminant":"False","Aligned.m.z..Da.":892.93532,"MH...Da.":1784.86305,"Theo..MH...Da.":1784.86022,"DeltaM..ppm.":1.59,"Deltam.z..Da.":0.00141,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":630223.6875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":15.0148,"First.Scan":14963,"Last.Scan":14963,"Master.Scan.s.":14957,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":1420,"XCorr":3.62,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":7.572e-08,"Percolator.SVMScore":3.46,"Precursor.Area":3877823,"Apex.RT..min.":15.04,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FTVSAIYSDLPQQER","Annotated.Sequence":"[K].FTVSAIYSDLPQQER.[D]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.69,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":877.44205,"Contaminant":"False","Aligned.m.z..Da.":877.4422,"MH...Da.":1753.87682,"Theo..MH...Da.":1753.87554,"DeltaM..ppm.":0.73,"Deltam.z..Da.":0.00064,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1274814.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":15.0129,"First.Scan":14960,"Last.Scan":14960,"Master.Scan.s.":14957,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":1069,"XCorr":3.71,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":6.156e-08,"Percolator.SVMScore":3.511,"Precursor.Area":2516119.25,"Apex.RT..min.":15.03,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FTVSAIYSDLPQQER","Annotated.Sequence":"[K].FTVSAIYSDLPQQER.[D]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.6382,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":585.29608,"Contaminant":"False","Aligned.m.z..Da.":585.29618,"MH...Da.":1753.87369,"Theo..MH...Da.":1753.87554,"DeltaM..ppm.":-1.05,"Deltam.z..Da.":-0.00062,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":397998.65625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":33.73302,"Ion.Inject.Time..ms.":22,"RT..min.":15.0172,"First.Scan":14967,"Last.Scan":14967,"Master.Scan.s.":14957,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":1150,"XCorr":2.93,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":0.00018,"Percolator.SVMScore":1.563,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FDDMELDENLLR","Annotated.Sequence":"[K].FDDmELDENLLR.[G]","Modifications":"M4(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6342,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":763.34416,"Contaminant":"False","Aligned.m.z..Da.":763.34435,"MH...Da.":1525.68104,"Theo..MH...Da.":1525.6839,"DeltaM..ppm.":-1.88,"Deltam.z..Da.":-0.00143,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3121258,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":84.88165,"Ion.Inject.Time..ms.":17.723,"RT..min.":14.4629,"First.Scan":14258,"Last.Scan":14258,"Master.Scan.s.":14255,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":957,"XCorr":2.57,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":5.423e-06,"Percolator.SVMScore":2.42,"Precursor.Area":4932367,"Apex.RT..min.":14.48,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].mSEGITDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7434,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1056.48579,"Contaminant":"False","Aligned.m.z..Da.":1056.48605,"MH...Da.":2111.9643,"Theo..MH...Da.":2111.96151,"DeltaM..ppm.":1.32,"Deltam.z..Da.":0.00139,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":488276.625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":14.609,"First.Scan":14448,"Last.Scan":14448,"Master.Scan.s.":14437,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":2324,"XCorr":5.3,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":7.708e-10,"Percolator.SVMScore":4.577,"Precursor.Area":2726954.75,"Apex.RT..min.":14.63,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].mSEGITDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.6168,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":704.65649,"Contaminant":"False","Aligned.m.z..Da.":704.65666,"MH...Da.":2111.95493,"Theo..MH...Da.":2111.96151,"DeltaM..ppm.":-3.12,"Deltam.z..Da.":-0.00219,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":776096.375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":23.97794,"Ion.Inject.Time..ms.":22,"RT..min.":14.6387,"First.Scan":14486,"Last.Scan":14486,"Master.Scan.s.":14476,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":2177,"XCorr":3.21,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":3.719e-06,"Percolator.SVMScore":2.512,"Precursor.Area":556576.75,"Apex.RT..min.":14.63,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FFISNPDLVDR","Annotated.Sequence":"[R].FFISNPDLVDR.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q03558","Master.Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","Protein.Accessions":"Q03558","Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7391,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":661.83868,"Contaminant":"False","Aligned.m.z..Da.":661.83884,"MH...Da.":1322.67009,"Theo..MH...Da.":1322.67393,"DeltaM..ppm.":-2.9,"Deltam.z..Da.":-0.00192,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1007059.0625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":26.81802,"Ion.Inject.Time..ms.":22,"RT..min.":14.659,"First.Scan":14512,"Last.Scan":14512,"Master.Scan.s.":14502,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":593,"XCorr":2.76,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":0.00006,"Percolator.SVMScore":1.84,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"HSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[R].HSDsYsENETNHTNVPISSTGGTNNK.[T]","Modifications":"S4(Phospho); S6(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.1486,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":984.05626,"Contaminant":"False","Aligned.m.z..Da.":984.05632,"MH...Da.":2950.15422,"Theo..MH...Da.":2950.15241,"DeltaM..ppm.":0.61,"Deltam.z..Da.":0.0006,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2858083.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":8.0254,"First.Scan":5813,"Last.Scan":5813,"Master.Scan.s.":5805,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":1153,"XCorr":6.93,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":3.645e-09,"Percolator.SVMScore":4.199,"Precursor.Area":11518434,"Apex.RT..min.":8.04,"ptmRS..Binomial.Peptide.Score":624.5,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(6): 739.21; y2+-H(3) O(4) P(23): 1257.03; y2+-H(3) O(4) P(22): 1173.53; y2+-H(3) O(4) P(21): 1092.00; b1+- -2H(3) O(4) P -2H(3) O(4) P(6): 641.23; b1+- -2H(3) O(4) P -2H(3) O(4) P(11): 1228.45; b2+- -2H(3) O(4) P -2H(3) O(4) P(13): 733.78; b2+- -2H(3) O(4) P -2H(3) O(4) P(14): 790.80; b2+- -2H(3) O(4) P -2H(3) O(4) P(15): 840.34","ptmRS..Best.Site.Probabilities":"S4(Phospho): 100; S6(Phospho): 99.99","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(4): 100.0; Y(5): 0.0; S(6): 100.0; T(10): 0.0; T(13): 0.0; S(18): 0.0; S(19): 0.0; T(20): 0.0; T(23): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LQETNPEEVPK","Annotated.Sequence":"[K].LQETNPEEVPK.[F]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":3,"DeltaScore":0.4965,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":642.32753,"Contaminant":"False","Aligned.m.z..Da.":642.32755,"MH...Da.":1283.64779,"Theo..MH...Da.":1283.64777,"DeltaM..ppm.":0.02,"Deltam.z..Da.":1e-05,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":30042276,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":11.21912,"Ion.Inject.Time..ms.":4.891,"RT..min.":7.6192,"First.Scan":5226,"Last.Scan":5226,"Master.Scan.s.":5220,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":526,"XCorr":2.86,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":0.00003,"Percolator.SVMScore":1.976,"Precursor.Area":22181234,"Apex.RT..min.":7.61,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"IGNNELLHR","Annotated.Sequence":"[K].IGNNELLHR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q03558","Master.Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","Protein.Accessions":"Q03558","Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6456,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":533.29316,"Contaminant":"False","Aligned.m.z..Da.":533.29318,"MH...Da.":1065.57905,"Theo..MH...Da.":1065.57997,"DeltaM..ppm.":-0.86,"Deltam.z..Da.":-0.00046,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2198169.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":57.49664,"Ion.Inject.Time..ms.":22,"RT..min.":7.6127,"First.Scan":5218,"Last.Scan":5218,"Master.Scan.s.":5207,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":302,"XCorr":2.06,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":0.00042,"Percolator.SVMScore":1.351,"Precursor.Area":1985071.25,"Apex.RT..min.":7.61,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"IQDPQMTGYVSTR","Annotated.Sequence":"[R].IQDPQmtGYVsTR.[Y]","Modifications":"M6(Oxidation); T7(Phospho); S11(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P32485","Master.Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","Protein.Accessions":"P32485","Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.0405,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":836.3271,"Contaminant":"False","Aligned.m.z..Da.":836.32713,"MH...Da.":1671.64692,"Theo..MH...Da.":1671.64853,"DeltaM..ppm.":-0.96,"Deltam.z..Da.":-0.0008,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":4231682,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":48.39535,"Ion.Inject.Time..ms.":22,"RT..min.":7.6474,"First.Scan":5266,"Last.Scan":5266,"Master.Scan.s.":5259,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":732,"XCorr":2.47,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":0.00045,"Percolator.SVMScore":1.335,"Precursor.Area":7466476.5,"Apex.RT..min.":7.68,"ptmRS..Binomial.Peptide.Score":345.19,"ptmRS..Isoform.Confidence.Probability":0.005,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(11): 1332.53; y1+-H(3) O(4) P(10): 1217.50; y1+-H(3) O(4) P(8): 992.39; y1+-H(3) O(4) P(7): 845.36; y2+-H(3) O(4) P(11): 666.77; y2+-H(3) O(4) P(10): 609.25; y1+(4): 462.27; y1+(3): 363.20","ptmRS..Best.Site.Probabilities":"T7(Phospho): 100; Y9(Phospho): 99.46","ptmRS..Phospho.Site.Probabilities":"T(7): 100.0; Y(9): 99.5; S(11): 0.5; T(12): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MFILDEADEMLSSGFK","Annotated.Sequence":"[K].mFILDEADEmLSSGFK.[E]","Modifications":"M1(Oxidation); M10(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7355,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":932.91923,"Contaminant":"False","Aligned.m.z..Da.":932.91918,"MH...Da.":1864.83119,"Theo..MH...Da.":1864.83433,"DeltaM..ppm.":-1.68,"Deltam.z..Da.":-0.00157,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":855890.625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":16.6877,"First.Scan":17105,"Last.Scan":17105,"Master.Scan.s.":17102,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":1921,"XCorr":3.97,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":2.261e-08,"Percolator.SVMScore":3.754,"Precursor.Area":2825497.25,"Apex.RT..min.":16.72,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MFILDEADEMLSSGFK","Annotated.Sequence":"[K].mFILDEADEmLSSGFK.[E]","Modifications":"M1(Oxidation); M10(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.629,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":622.28232,"Contaminant":"False","Aligned.m.z..Da.":622.28229,"MH...Da.":1864.83241,"Theo..MH...Da.":1864.83433,"DeltaM..ppm.":-1.03,"Deltam.z..Da.":-0.00064,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1340973.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":16.6977,"First.Scan":17118,"Last.Scan":17118,"Master.Scan.s.":17115,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":1895,"XCorr":3.1,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":0.00004,"Percolator.SVMScore":1.954,"Precursor.Area":1669142.875,"Apex.RT..min.":16.72,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LQETNPEEVPK","Annotated.Sequence":"[K].LQETNPEEVPK.[F]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5117,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":642.32821,"Contaminant":"False","Aligned.m.z..Da.":642.32823,"MH...Da.":1283.64915,"Theo..MH...Da.":1283.64777,"DeltaM..ppm.":1.08,"Deltam.z..Da.":0.00069,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3928662.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":7.571,"First.Scan":5159,"Last.Scan":5159,"Master.Scan.s.":5155,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":521,"XCorr":2.56,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":0.00059,"Percolator.SVMScore":1.269,"Precursor.Area":22181234,"Apex.RT..min.":7.61,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"IQDPQMTGYVSTR","Annotated.Sequence":"[R].IQDPQmTGyVSTR.[Y]","Modifications":"M6(Oxidation); Y9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P32485","Master.Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","Protein.Accessions":"P32485","Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1201,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":796.34531,"Contaminant":"False","Aligned.m.z..Da.":796.34533,"MH...Da.":1591.68335,"Theo..MH...Da.":1591.6822,"DeltaM..ppm.":0.72,"Deltam.z..Da.":0.00057,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2178882.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":7.5561,"First.Scan":5140,"Last.Scan":5140,"Master.Scan.s.":5129,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":1221,"XCorr":3.08,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":7.19e-06,"Percolator.SVMScore":2.352,"Precursor.Area":12787358,"Apex.RT..min.":7.61,"ptmRS..Binomial.Peptide.Score":281.42,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+(6): 762.32; y1+(5): 705.30","ptmRS..Best.Site.Probabilities":"Y9(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"T(7): 0.0; Y(9): 100.0; S(11): 0.0; T(12): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"HSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[R].HSDSySENETNHTNVPISSTGGTNNK.[T]","Modifications":"Y5(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":0,"Charge":4,"Original.Precursor.Charge":4,"DeltaScore":0.0116,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":718.30063,"Contaminant":"False","Aligned.m.z..Da.":718.30065,"MH...Da.":2870.18071,"Theo..MH...Da.":2870.18608,"DeltaM..ppm.":-1.87,"Deltam.z..Da.":-0.00134,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2014989.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":9.67475,"Ion.Inject.Time..ms.":22,"RT..min.":7.5533,"First.Scan":5135,"Last.Scan":5135,"Master.Scan.s.":5129,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":2367,"XCorr":6.04,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":1.064e-08,"Percolator.SVMScore":3.938,"Precursor.Area":7550528.5,"Apex.RT..min.":7.57,"ptmRS..Binomial.Peptide.Score":273.83,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(10): 1132.42; b1+-H(3) O(4) P(11): 1246.46; b1+-H(3) O(4) P(12): 1383.52; b2+-H(3) O(4) P(7): 394.65; b2+-H(3) O(4) P(8): 451.67; b2+-H(3) O(4) P(13): 742.79; b2+-H(3) O(4) P(14): 799.81; b2+-H(3) O(4) P(15): 849.34; b2+-H(3) O(4) P(17): 954.41; b1+(4): 427.16","ptmRS..Best.Site.Probabilities":"S6(Phospho): 99.25","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(4): 0.7; Y(5): 0.0; S(6): 99.3; T(10): 0.0; T(13): 0.0; S(18): 0.0; S(19): 0.0; T(20): 0.0; T(23): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSSPLHRLSPTGK","Annotated.Sequence":"[R].mSsPLHRLsPTGK.[S]","Modifications":"M1(Oxidation); S3(Phospho); S9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.5016,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":529.56507,"Contaminant":"False","Aligned.m.z..Da.":529.56509,"MH...Da.":1586.68066,"Theo..MH...Da.":1586.67977,"DeltaM..ppm.":0.56,"Deltam.z..Da.":0.0003,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2664033.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":18.67764,"Ion.Inject.Time..ms.":22,"RT..min.":7.6756,"First.Scan":5306,"Last.Scan":5306,"Master.Scan.s.":5298,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":1081,"XCorr":3.17,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":0.00028,"Percolator.SVMScore":1.452,"Precursor.Area":15038191,"Apex.RT..min.":7.72,"ptmRS..Binomial.Peptide.Score":226.68,"ptmRS..Isoform.Confidence.Probability":0.99,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(8): 877.50; y1+-H(3) O(4) P(7): 740.44; y2+-H(3) O(4) P(12): 671.34; y2+-H(3) O(4) P(10): 544.32; y2+-H(3) O(4) P(8): 439.25; y1+(4): 402.23","ptmRS..Best.Site.Probabilities":"S3(Phospho): 100; S9(Phospho): 99.04","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(3): 100.0; S(9): 99.0; T(11): 1.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSSPLHRLSPTGK","Annotated.Sequence":"[R].mSsPLHRLsPTGK.[S]","Modifications":"M1(Oxidation); S3(Phospho); S9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.4789,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":793.84352,"Contaminant":"False","Aligned.m.z..Da.":793.84355,"MH...Da.":1586.67976,"Theo..MH...Da.":1586.67977,"DeltaM..ppm.":-0.01,"Deltam.z..Da.":-1e-05,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2413253.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":39.56829,"Ion.Inject.Time..ms.":22,"RT..min.":7.6946,"First.Scan":5333,"Last.Scan":5333,"Master.Scan.s.":5324,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":973,"XCorr":1.9,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":0.00048,"Percolator.SVMScore":1.32,"Precursor.Area":4913041,"Apex.RT..min.":7.72,"ptmRS..Binomial.Peptide.Score":296.75,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(3): 304.10; y2+-H(3) O(4) P(11): 627.82; y2+- -2H(3) O(4) P -2H(3) O(4) P(11): 578.83; b1+(2): 235.07","ptmRS..Best.Site.Probabilities":"S3(Phospho): 100; S9(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(3): 100.0; S(9): 100.0; T(11): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LEAELAESK","Annotated.Sequence":"[K].LEAELAESK.[Q]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.3571,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":495.26098,"Contaminant":"False","Aligned.m.z..Da.":495.261,"MH...Da.":989.51469,"Theo..MH...Da.":989.51497,"DeltaM..ppm.":-0.28,"Deltam.z..Da.":-0.00014,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3107701.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":43.90775,"Ion.Inject.Time..ms.":22,"RT..min.":7.7469,"First.Scan":5407,"Last.Scan":5407,"Master.Scan.s.":5402,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":367,"XCorr":2.38,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":0.00034,"Percolator.SVMScore":1.403,"Precursor.Area":5810614,"Apex.RT..min.":7.76,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSSPLHRLSPTGK","Annotated.Sequence":"[R].MSsPLHRLsPTGK.[S]","Modifications":"S3(Phospho); S9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.5934,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":524.23296,"Contaminant":"False","Aligned.m.z..Da.":524.233,"MH...Da.":1570.68433,"Theo..MH...Da.":1570.68486,"DeltaM..ppm.":-0.33,"Deltam.z..Da.":-0.00017,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2783873,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":28.61896,"Ion.Inject.Time..ms.":22,"RT..min.":8.6472,"First.Scan":6724,"Last.Scan":6724,"Master.Scan.s.":6715,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":1073,"XCorr":3.64,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":0.00052,"Percolator.SVMScore":1.298,"Precursor.Area":8857661,"Apex.RT..min.":8.68,"ptmRS..Binomial.Peptide.Score":269.65,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y2+- -2H(3) O(4) P -2H(3) O(4) P(12): 622.35; y2+- -2H(3) O(4) P -2H(3) O(4) P(11): 578.83; b1+(2): 219.08","ptmRS..Best.Site.Probabilities":"S3(Phospho): 99.99; S9(Phospho): 99.99","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(3): 100.0; S(9): 100.0; T(11): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"NQQLEDEIEK","Annotated.Sequence":"[K].NQQLEDEIEK.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5882,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":623.30195,"Contaminant":"False","Aligned.m.z..Da.":623.302,"MH...Da.":1245.59663,"Theo..MH...Da.":1245.59574,"DeltaM..ppm.":0.72,"Deltam.z..Da.":0.00045,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3047238.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":64.59737,"Ion.Inject.Time..ms.":15.752,"RT..min.":8.5745,"First.Scan":6619,"Last.Scan":6619,"Master.Scan.s.":6611,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":580,"XCorr":2.38,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":0.00023,"Percolator.SVMScore":1.501,"Precursor.Area":3575595.5,"Apex.RT..min.":8.58,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"NQQLEDEIEK","Annotated.Sequence":"[K].NQQLEDEIEK.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.4091,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":623.30128,"Contaminant":"False","Aligned.m.z..Da.":623.30133,"MH...Da.":1245.59529,"Theo..MH...Da.":1245.59574,"DeltaM..ppm.":-0.36,"Deltam.z..Da.":-0.00023,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1482165.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":80.83388,"Ion.Inject.Time..ms.":22,"RT..min.":8.5655,"First.Scan":6606,"Last.Scan":6606,"Master.Scan.s.":6598,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":639,"XCorr":1.76,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":0.00045,"Percolator.SVMScore":1.334,"Precursor.Area":3575595.5,"Apex.RT..min.":8.58,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FLQNPLEIFVDDEAK","Annotated.Sequence":"[R].FLQNPLEIFVDDEAK.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.715,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":889.45334,"Contaminant":"False","Aligned.m.z..Da.":889.45346,"MH...Da.":1777.89941,"Theo..MH...Da.":1777.90069,"DeltaM..ppm.":-0.72,"Deltam.z..Da.":-0.00064,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":513152.78125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":18.5553,"First.Scan":19502,"Last.Scan":19502,"Master.Scan.s.":19494,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":961,"XCorr":3.79,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":5.774e-08,"Percolator.SVMScore":3.526,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.4912,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40404,"Contaminant":"False","Aligned.m.z..Da.":967.40421,"MH...Da.":2900.19757,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":-0.61,"Deltam.z..Da.":-0.00059,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":87652.42188,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":22.29455,"Ion.Inject.Time..ms.":22,"RT..min.":18.8176,"First.Scan":19842,"Last.Scan":19842,"Master.Scan.s.":19832,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":2405,"XCorr":3.4,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":1.448e-06,"Percolator.SVMScore":2.742,"Precursor.Area":118889.10156,"Apex.RT..min.":19.16,"ptmRS..Binomial.Peptide.Score":282.08,"ptmRS..Isoform.Confidence.Probability":0.5,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"Y12(Phospho): 50; S13(Phospho): 50","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 50.0; S(13): 50.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DIFSNDELLSDAYDAK","Annotated.Sequence":"[K].DIFSNDELLSDAYDAK.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6953,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":908.41971,"Contaminant":"False","Aligned.m.z..Da.":908.41967,"MH...Da.":1815.83214,"Theo..MH...Da.":1815.82831,"DeltaM..ppm.":2.11,"Deltam.z..Da.":0.00191,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":386839.28125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":17.0658,"First.Scan":17592,"Last.Scan":17592,"Master.Scan.s.":17583,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":1695,"XCorr":3.84,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":1.54e-08,"Percolator.SVMScore":3.848,"Precursor.Area":9251618,"Apex.RT..min.":17.1,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DIFSNDELLSDAYDAK","Annotated.Sequence":"[K].DIFSNDELLSDAYDAK.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.7042,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":605.94857,"Contaminant":"False","Aligned.m.z..Da.":605.94855,"MH...Da.":1815.83117,"Theo..MH...Da.":1815.82831,"DeltaM..ppm.":1.57,"Deltam.z..Da.":0.00095,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":579238.875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":18.24674,"Ion.Inject.Time..ms.":22,"RT..min.":17.0729,"First.Scan":17600,"Last.Scan":17600,"Master.Scan.s.":17596,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":1679,"XCorr":2.84,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":0.00009,"Percolator.SVMScore":1.723,"Precursor.Area":2252226.25,"Apex.RT..min.":17.1,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DIFSNDELLSDAYDAK","Annotated.Sequence":"[K].DIFSNDELLSDAYDAK.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":2,"DeltaScore":0.6763,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":605.94829,"Contaminant":"False","Aligned.m.z..Da.":605.94826,"MH...Da.":1815.8303,"Theo..MH...Da.":1815.82831,"DeltaM..ppm.":1.09,"Deltam.z..Da.":0.00066,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3448413.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":11.6157,"Ion.Inject.Time..ms.":22,"RT..min.":17.1046,"First.Scan":17641,"Last.Scan":17641,"Master.Scan.s.":17635,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":1695,"XCorr":3.12,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":0.00001,"Percolator.SVMScore":2.176,"Precursor.Area":2252226.25,"Apex.RT..min.":17.1,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0123,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40377,"Contaminant":"False","Aligned.m.z..Da.":967.40373,"MH...Da.":2900.19677,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":-0.88,"Deltam.z..Da.":-0.00085,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":594233.9375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":35.05713,"Ion.Inject.Time..ms.":22,"RT..min.":16.8109,"First.Scan":17264,"Last.Scan":17264,"Master.Scan.s.":17258,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":2347,"XCorr":4.07,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":4.072e-06,"Percolator.SVMScore":2.49,"ptmRS..Binomial.Peptide.Score":336.57,"ptmRS..Isoform.Confidence.Probability":0.99,"ptmRS..Isoform.Group.Report":"y2+-H(3) O(4) P(13): 729.84","ptmRS..Best.Site.Probabilities":"S13(Phospho): 99.04","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 1.0; S(13): 99.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"EDGTTPFVAIWK","Annotated.Sequence":"[R].EDGTTPFVAIWK.[H]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6757,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":682.34849,"Contaminant":"False","Aligned.m.z..Da.":682.34846,"MH...Da.":1363.6897,"Theo..MH...Da.":1363.68924,"DeltaM..ppm.":0.33,"Deltam.z..Da.":0.00023,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":664825.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":29.93186,"Ion.Inject.Time..ms.":22,"RT..min.":16.9217,"First.Scan":17406,"Last.Scan":17406,"Master.Scan.s.":17401,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":659,"XCorr":2.22,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":0.00008,"Percolator.SVMScore":1.772,"Precursor.Area":783005.625,"Apex.RT..min.":16.93,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MFILDEADEMLSSGFK","Annotated.Sequence":"[K].mFILDEADEmLSSGFK.[E]","Modifications":"M1(Oxidation); M10(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":3,"DeltaScore":0.636,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":932.92178,"Contaminant":"False","Aligned.m.z..Da.":932.92174,"MH...Da.":1864.83629,"Theo..MH...Da.":1864.83433,"DeltaM..ppm.":1.05,"Deltam.z..Da.":0.00098,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1219885,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":57.73878,"Ion.Inject.Time..ms.":22,"RT..min.":16.8514,"First.Scan":17316,"Last.Scan":17316,"Master.Scan.s.":17310,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":1860,"XCorr":2.39,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":0.00002,"Percolator.SVMScore":2.115,"Precursor.Area":2446339.75,"Apex.RT..min.":16.81,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"SQMSVELDDDADLDDELAQLK","Annotated.Sequence":"[R].SQmsVELDDDADLDDELAQLK.[G]","Modifications":"M3(Oxidation); S4(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q08421","Master.Protein.Descriptions":"Enhancer of translation termination 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ETT1 PE=1 SV=1","Protein.Accessions":"Q08421","Protein.Descriptions":"Enhancer of translation termination 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ETT1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0845,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":816.01106,"Contaminant":"False","Aligned.m.z..Da.":816.01105,"MH...Da.":2446.01863,"Theo..MH...Da.":2446.02149,"DeltaM..ppm.":-1.17,"Deltam.z..Da.":-0.00095,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":442620.84375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":16.79259,"Ion.Inject.Time..ms.":22,"RT..min.":17.4613,"First.Scan":18098,"Last.Scan":18098,"Master.Scan.s.":18090,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":1992,"XCorr":3.43,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":0.00001,"Percolator.SVMScore":2.202,"ptmRS..Binomial.Peptide.Score":329.16,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(4): 432.15; b1+-H(3) O(4) P(5): 531.22; b1+-H(3) O(4) P(6): 660.27; b1+-H(3) O(4) P(7): 773.35; b1+(2): 216.10; b1+(3): 363.13","ptmRS..Best.Site.Probabilities":"S4(Phospho): 99.99","ptmRS..Phospho.Site.Probabilities":"S(1): 0.0; S(4): 100.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0592,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40311,"Contaminant":"False","Aligned.m.z..Da.":967.40309,"MH...Da.":2900.19479,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":-1.56,"Deltam.z..Da.":-0.00151,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":668910.5625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":17.3411,"First.Scan":17945,"Last.Scan":17945,"Master.Scan.s.":17934,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":2260,"XCorr":4.9,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":2.236e-07,"Percolator.SVMScore":3.196,"ptmRS..Binomial.Peptide.Score":403.83,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(14): 1621.73; b2+-H(3) O(4) P(15): 821.83; b2+-H(3) O(4) P(18): 1014.90; b1+(12): 1344.56","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KDELTLEGIK","Annotated.Sequence":"[K].KDELTLEGIK.[Q]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5766,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":573.32309,"Contaminant":"False","Aligned.m.z..Da.":573.32314,"MH...Da.":1145.63891,"Theo..MH...Da.":1145.64123,"DeltaM..ppm.":-2.03,"Deltam.z..Da.":-0.00116,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3851441,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":15.74054,"Ion.Inject.Time..ms.":22,"RT..min.":10.2862,"First.Scan":8917,"Last.Scan":8917,"Master.Scan.s.":8912,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":364,"XCorr":3.85,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":1.793e-06,"Percolator.SVMScore":2.69,"Precursor.Area":9861767,"Apex.RT..min.":10.3,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"APQALMLAPTR","Annotated.Sequence":"[K].APQALmLAPTR.[E]","Modifications":"M6(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7206,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":592.82619,"Contaminant":"False","Aligned.m.z..Da.":592.82624,"MH...Da.":1184.64511,"Theo..MH...Da.":1184.6456,"DeltaM..ppm.":-0.42,"Deltam.z..Da.":-0.00025,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3922986.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":12.39546,"Ion.Inject.Time..ms.":22,"RT..min.":10.1679,"First.Scan":8764,"Last.Scan":8764,"Master.Scan.s.":8756,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":325,"XCorr":3.4,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":5.149e-06,"Percolator.SVMScore":2.433,"Precursor.Area":10479479,"Apex.RT..min.":10.19,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DAQIVVGTPGR","Annotated.Sequence":"[R].DAQIVVGTPGR.[V]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6779,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":556.806,"Contaminant":"False","Aligned.m.z..Da.":556.80603,"MH...Da.":1112.60472,"Theo..MH...Da.":1112.60585,"DeltaM..ppm.":-1.01,"Deltam.z..Da.":-0.00056,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3135298,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":9.48489,"Ion.Inject.Time..ms.":22,"RT..min.":9.1646,"First.Scan":7444,"Last.Scan":7444,"Master.Scan.s.":7443,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":261,"XCorr":2.67,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":0.00055,"Percolator.SVMScore":1.285,"Precursor.Area":2512505.75,"Apex.RT..min.":9.15,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"DFRHSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[K].DFRHsDsYSENETNHTNVPISSTGGTNNK.[T]","Modifications":"S5(Phospho); S7(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0142,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1123.45484,"Contaminant":"False","Aligned.m.z..Da.":1123.45492,"MH...Da.":3368.34996,"Theo..MH...Da.":3368.34888,"DeltaM..ppm.":0.32,"Deltam.z..Da.":0.00036,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2719102.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":8.7097,"First.Scan":6813,"Last.Scan":6813,"Master.Scan.s.":6806,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":953,"XCorr":6.34,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":5.953e-09,"Percolator.SVMScore":4.079,"Precursor.Area":2775881.5,"Apex.RT..min.":8.7,"ptmRS..Binomial.Peptide.Score":599.72,"ptmRS..Isoform.Confidence.Probability":0.992,"ptmRS..Isoform.Group.Report":"y2+-H(3) O(4) P(24): 1274.56; y2+-H(3) O(4) P(23): 1217.05; b1+- -2H(3) O(4) P -2H(3) O(4) P(12): 1431.56; b1+- -2H(3) O(4) P -2H(3) O(4) P(13): 1532.60; b1+- -2H(3) O(4) P -2H(3) O(4) P(14): 1646.65; b2+- -2H(3) O(4) P -2H(3) O(4) P(7): 405.17; b2+- -2H(3) O(4) P -2H(3) O(4) P(15): 892.36; b2+- -2H(3) O(4) P -2H(3) O(4) P(16): 942.88; b2+- -2H(3) O(4) P -2H(3) O(4) P(17): 999.90; b2+- -2H(3) O(4) P -2H(3) O(4) P(18): 1049.44; b2+- -2H(3) O(4) P -2H(3) O(4) P(20): 1154.50; b2+- -2H(3) O(4) P -2H(3) O(4) P(27): 1456.63","ptmRS..Best.Site.Probabilities":"S5(Phospho): 100; S7(Phospho): 99.21","ptmRS..Phospho.Site.Probabilities":"S(5): 100.0; S(7): 99.2; Y(8): 0.0; S(9): 0.8; T(13): 0.0; T(16): 0.0; S(21): 0.0; S(22): 0.0; T(23): 0.0; T(26): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LQETNPEEVPKFEK","Annotated.Sequence":"[K].LQETNPEEVPKFEK.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.7317,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":563.28883,"Contaminant":"False","Aligned.m.z..Da.":563.28886,"MH...Da.":1687.85194,"Theo..MH...Da.":1687.85374,"DeltaM..ppm.":-1.07,"Deltam.z..Da.":-0.0006,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1648340.875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":75.16302,"Ion.Inject.Time..ms.":19.088,"RT..min.":9.3442,"First.Scan":7688,"Last.Scan":7688,"Master.Scan.s.":7677,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":880,"XCorr":2.46,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":0.00005,"Percolator.SVMScore":1.856,"Precursor.Area":6084430.5,"Apex.RT..min.":9.36,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (N4)","Search.ID":"N","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"AAEAGETGAATSATEGDNNNNTAAGDKK","Annotated.Sequence":"[K].AAEAGETGAATSATEGDNNNNTAAGDKK.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.7617,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":879.39378,"Contaminant":"False","Aligned.m.z..Da.":879.39383,"MH...Da.":2636.1668,"Theo..MH...Da.":2636.16665,"DeltaM..ppm.":0.06,"Deltam.z..Da.":0.00005,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1986715,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":4.9631,"First.Scan":1868,"Last.Scan":1868,"Master.Scan.s.":1862,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_05.raw","File.ID":"F23","Quan.Info":"","Peptides.Matched":3561,"XCorr":8.31,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":2.10501e-16,"Percolator.SVMScore":10.69479,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (N4)","Search.ID":"N","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":5,"DeltaScore":0.0585,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.4049,"Contaminant":"False","Aligned.m.z..Da.":967.40487,"MH...Da.":2900.20014,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":0.28,"Deltam.z..Da.":0.00027,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3703872,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":3.67157,"Ion.Inject.Time..ms.":22,"RT..min.":14.983,"First.Scan":15198,"Last.Scan":15198,"Master.Scan.s.":15195,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_05.raw","File.ID":"F23","Quan.Info":"","Peptides.Matched":2493,"XCorr":6.84,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":6.871e-16,"Percolator.SVMScore":7.268,"Precursor.Area":5776421.5,"Apex.RT..min.":14.96,"ptmRS..Binomial.Peptide.Score":795.65,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(13): 1413.58; b1+-H(3) O(4) P(14): 1528.61; b1+-H(3) O(4) P(15): 1642.65; b1+-H(3) O(4) P(16): 1771.69; b1+-H(3) O(4) P(17): 1899.75; y1+-H(3) O(4) P(15): 1750.78; y1+-H(3) O(4) P(14): 1621.73; y1+-H(3) O(4) P(13): 1458.67; b2+-H(3) O(4) P(16): 886.35; b2+-H(3) O(4) P(18): 1014.90; y2+-H(3) O(4) P(14): 811.37; b1+(12): 1344.56; y1+(13): 1556.65","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (N4)","Search.ID":"N","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FTVSAIYSDLPQQER","Annotated.Sequence":"[K].FTVSAIYSDLPQQER.[D]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6875,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":877.44299,"Contaminant":"False","Aligned.m.z..Da.":877.44297,"MH...Da.":1753.87872,"Theo..MH...Da.":1753.87554,"DeltaM..ppm.":1.81,"Deltam.z..Da.":0.00159,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":259464.29688,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":29.67912,"Ion.Inject.Time..ms.":22,"RT..min.":14.9761,"First.Scan":15192,"Last.Scan":15192,"Master.Scan.s.":15189,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_05.raw","File.ID":"F23","Quan.Info":"","Peptides.Matched":1116,"XCorr":3.52,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":3.099e-09,"Percolator.SVMScore":3.468,"Precursor.Area":257242.51562,"Apex.RT..min.":14.99,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (N4)","Search.ID":"N","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FFISNPDLVDRLEK","Annotated.Sequence":"[R].FFISNPDLVDRLEK.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q03558","Master.Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","Protein.Accessions":"Q03558","Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.5479,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":564.96821,"Contaminant":"False","Aligned.m.z..Da.":564.96811,"MH...Da.":1692.89009,"Theo..MH...Da.":1692.89555,"DeltaM..ppm.":-3.22,"Deltam.z..Da.":-0.00182,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":519126.21875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":60.11491,"Ion.Inject.Time..ms.":22,"RT..min.":15.2975,"First.Scan":15569,"Last.Scan":15569,"Master.Scan.s.":15557,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_05.raw","File.ID":"F23","Quan.Info":"","Peptides.Matched":579,"XCorr":1.46,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":0.00027,"Percolator.SVMScore":1.28,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (N4)","Search.ID":"N","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"GVFGYGFEEPSAIQQR","Annotated.Sequence":"[R].GVFGYGFEEPSAIQQR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.4863,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":892.93433,"Contaminant":"False","Aligned.m.z..Da.":892.93425,"MH...Da.":1784.86139,"Theo..MH...Da.":1784.86022,"DeltaM..ppm.":0.65,"Deltam.z..Da.":0.00058,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":354277.15625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":45.59838,"Ion.Inject.Time..ms.":22,"RT..min.":15.1052,"First.Scan":15327,"Last.Scan":15327,"Master.Scan.s.":15319,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_05.raw","File.ID":"F23","Quan.Info":"","Peptides.Matched":1386,"XCorr":1.83,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":0.00016,"Percolator.SVMScore":1.383,"Precursor.Area":708240.125,"Apex.RT..min.":15.08,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (N4)","Search.ID":"N","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"GVFGYGFEEPSAIQQR","Annotated.Sequence":"[R].GVFGYGFEEPSAIQQR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6621,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":892.93348,"Contaminant":"False","Aligned.m.z..Da.":892.93343,"MH...Da.":1784.8597,"Theo..MH...Da.":1784.86022,"DeltaM..ppm.":-0.29,"Deltam.z..Da.":-0.00026,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":434231.375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":15.68257,"Ion.Inject.Time..ms.":22,"RT..min.":15.0592,"First.Scan":15268,"Last.Scan":15268,"Master.Scan.s.":15264,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_05.raw","File.ID":"F23","Quan.Info":"","Peptides.Matched":1293,"XCorr":4.35,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":2.769e-10,"Percolator.SVMScore":3.933,"Precursor.Area":708240.125,"Apex.RT..min.":15.08,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (N4)","Search.ID":"N","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"GVFGYGFEEPSAIQQR","Annotated.Sequence":"[R].GVFGYGFEEPSAIQQR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.495,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":595.62411,"Contaminant":"False","Aligned.m.z..Da.":595.62407,"MH...Da.":1784.85779,"Theo..MH...Da.":1784.86022,"DeltaM..ppm.":-1.36,"Deltam.z..Da.":-0.00081,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":239852.71875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":29.17007,"Ion.Inject.Time..ms.":22,"RT..min.":15.0707,"First.Scan":15284,"Last.Scan":15284,"Master.Scan.s.":15277,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_05.raw","File.ID":"F23","Quan.Info":"","Peptides.Matched":1380,"XCorr":2.02,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":0.00027,"Percolator.SVMScore":1.279,"Precursor.Area":167504.67188,"Apex.RT..min.":15.08,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (N4)","Search.ID":"N","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FDDMELDENLLR","Annotated.Sequence":"[K].FDDmELDENLLR.[G]","Modifications":"M4(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5981,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":763.3458,"Contaminant":"False","Aligned.m.z..Da.":763.34586,"MH...Da.":1525.68433,"Theo..MH...Da.":1525.6839,"DeltaM..ppm.":0.28,"Deltam.z..Da.":0.00021,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":536561.5625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":15.42759,"Ion.Inject.Time..ms.":22,"RT..min.":14.5093,"First.Scan":14673,"Last.Scan":14673,"Master.Scan.s.":14670,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_05.raw","File.ID":"F23","Quan.Info":"","Peptides.Matched":1088,"XCorr":3.16,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":7.161e-07,"Percolator.SVMScore":2.42,"Precursor.Area":1115861.5,"Apex.RT..min.":14.56,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (N4)","Search.ID":"N","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"TAVFYGGTPISK","Annotated.Sequence":"[K].TAVFYGGTPISK.[D]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5391,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":620.83237,"Contaminant":"False","Aligned.m.z..Da.":620.8323,"MH...Da.":1240.65747,"Theo..MH...Da.":1240.65721,"DeltaM..ppm.":0.21,"Deltam.z..Da.":0.00013,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":357988.59375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":68.97018,"Ion.Inject.Time..ms.":22,"RT..min.":11.2157,"First.Scan":10634,"Last.Scan":10634,"Master.Scan.s.":10624,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_05.raw","File.ID":"F23","Quan.Info":"","Peptides.Matched":385,"XCorr":2.56,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":0.00007,"Percolator.SVMScore":1.534,"Precursor.Area":482575.1875,"Apex.RT..min.":11.23,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (N4)","Search.ID":"N","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"TAVFYGGTPISK","Annotated.Sequence":"[K].TAVFYGGTPISK.[D]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5353,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":620.8317,"Contaminant":"False","Aligned.m.z..Da.":620.83163,"MH...Da.":1240.65614,"Theo..MH...Da.":1240.65721,"DeltaM..ppm.":-0.87,"Deltam.z..Da.":-0.00054,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":572246.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":54.52758,"Ion.Inject.Time..ms.":22,"RT..min.":11.225,"First.Scan":10647,"Last.Scan":10647,"Master.Scan.s.":10637,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_05.raw","File.ID":"F23","Quan.Info":"","Peptides.Matched":418,"XCorr":2.69,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":2.078e-06,"Percolator.SVMScore":2.215,"Precursor.Area":482575.1875,"Apex.RT..min.":11.23,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (N4)","Search.ID":"N","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].mSEGITDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6113,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1056.48438,"Contaminant":"False","Aligned.m.z..Da.":1056.48446,"MH...Da.":2111.96149,"Theo..MH...Da.":2111.96151,"DeltaM..ppm.":-0.01,"Deltam.z..Da.":-1e-05,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":751527.4375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":62.31062,"Ion.Inject.Time..ms.":22,"RT..min.":14.5711,"First.Scan":14751,"Last.Scan":14751,"Master.Scan.s.":14748,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_05.raw","File.ID":"F23","Quan.Info":"","Peptides.Matched":2276,"XCorr":3.73,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":8.927e-10,"Percolator.SVMScore":3.708,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (N4)","Search.ID":"N","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FDDMELDENLLR","Annotated.Sequence":"[K].FDDmELDENLLR.[G]","Modifications":"M4(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":3,"DeltaScore":0.6058,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":763.345,"Contaminant":"False","Aligned.m.z..Da.":763.34505,"MH...Da.":1525.68271,"Theo..MH...Da.":1525.6839,"DeltaM..ppm.":-0.78,"Deltam.z..Da.":-0.00059,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":875375.6875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":41.91447,"Ion.Inject.Time..ms.":22,"RT..min.":14.5953,"First.Scan":14783,"Last.Scan":14783,"Master.Scan.s.":14774,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_05.raw","File.ID":"F23","Quan.Info":"","Peptides.Matched":1103,"XCorr":2.74,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":2.323e-07,"Percolator.SVMScore":2.637,"Precursor.Area":1115861.5,"Apex.RT..min.":14.56,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (N4)","Search.ID":"N","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LQETNPEEVPKFEK","Annotated.Sequence":"[K].LQETNPEEVPKFEK.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.6,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":563.29046,"Contaminant":"False","Aligned.m.z..Da.":563.29049,"MH...Da.":1687.85683,"Theo..MH...Da.":1687.85374,"DeltaM..ppm.":1.83,"Deltam.z..Da.":0.00103,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2273951.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":53.80639,"Ion.Inject.Time..ms.":7.445,"RT..min.":9.3172,"First.Scan":8032,"Last.Scan":8032,"Master.Scan.s.":8024,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_05.raw","File.ID":"F23","Quan.Info":"","Peptides.Matched":893,"XCorr":1.4,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":0.00038,"Percolator.SVMScore":1.213,"Precursor.Area":1885048.875,"Apex.RT..min.":9.32,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (N4)","Search.ID":"N","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FDDMELDENLLR","Annotated.Sequence":"[K].FDDMELDENLLR.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5277,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":755.34583,"Contaminant":"False","Aligned.m.z..Da.":755.34556,"MH...Da.":1509.68439,"Theo..MH...Da.":1509.68898,"DeltaM..ppm.":-3.04,"Deltam.z..Da.":-0.0023,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":335597.96875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":52.1895,"Ion.Inject.Time..ms.":22,"RT..min.":16.0729,"First.Scan":16466,"Last.Scan":16466,"Master.Scan.s.":16462,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_05.raw","File.ID":"F23","Quan.Info":"","Peptides.Matched":947,"XCorr":3.07,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":2.697e-07,"Percolator.SVMScore":2.608,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (N4)","Search.ID":"N","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].msEGITDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S2(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1507,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1096.46986,"Contaminant":"False","Aligned.m.z..Da.":1096.46947,"MH...Da.":2191.93246,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":2.11,"Deltam.z..Da.":0.00231,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":84569.66406,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":52.5636,"Ion.Inject.Time..ms.":22,"RT..min.":16.0597,"First.Scan":16453,"Last.Scan":16453,"Master.Scan.s.":16447,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_05.raw","File.ID":"F23","Quan.Info":"","Peptides.Matched":2110,"XCorr":2.72,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":0.00002,"Percolator.SVMScore":1.765,"Precursor.Area":5000513.5,"Apex.RT..min.":15.56,"ptmRS..Binomial.Peptide.Score":334.49,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(2): 112.04; b1+-H(3) O(4) P(3): 241.08; b1+-H(3) O(4) P(4): 298.10; b1+-H(3) O(4) P(5): 411.19; b1+-H(3) O(4) P(6): 512.24; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+-H(3) O(4) P(9): 869.39; b1+-H(3) O(4) P(10): 998.43","ptmRS..Best.Site.Probabilities":"S2(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 100.0; T(6): 0.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (N4)","Search.ID":"N","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MFILDEADEMLSSGFK","Annotated.Sequence":"[K].mFILDEADEmLSSGFK.[E]","Modifications":"M1(Oxidation); M10(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.63,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":932.92206,"Contaminant":"False","Aligned.m.z..Da.":932.92179,"MH...Da.":1864.83685,"Theo..MH...Da.":1864.83433,"DeltaM..ppm.":1.35,"Deltam.z..Da.":0.00126,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":224817.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":3.38994,"Ion.Inject.Time..ms.":22,"RT..min.":16.6952,"First.Scan":17027,"Last.Scan":17027,"Master.Scan.s.":17026,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_05.raw","File.ID":"F23","Quan.Info":"","Peptides.Matched":1900,"XCorr":3.27,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":4.094e-09,"Percolator.SVMScore":3.415,"Precursor.Area":432302.46875,"Apex.RT..min.":16.81,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (N4)","Search.ID":"N","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MFILDEADEMLSSGFK","Annotated.Sequence":"[K].mFILDEADEmLSSGFK.[E]","Modifications":"M1(Oxidation); M10(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.6764,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":622.28183,"Contaminant":"False","Aligned.m.z..Da.":622.28165,"MH...Da.":1864.83093,"Theo..MH...Da.":1864.83433,"DeltaM..ppm.":-1.82,"Deltam.z..Da.":-0.00113,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":221708.10938,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":16.7825,"First.Scan":17079,"Last.Scan":17079,"Master.Scan.s.":17077,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_05.raw","File.ID":"F23","Quan.Info":"","Peptides.Matched":1871,"XCorr":2.75,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":0.00004,"Percolator.SVMScore":1.629,"Precursor.Area":228021.0625,"Apex.RT..min.":16.81,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (N4)","Search.ID":"N","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.437,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40852,"Contaminant":"False","Aligned.m.z..Da.":967.40821,"MH...Da.":2900.21101,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":4.03,"Deltam.z..Da.":0.00389,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":107710.99219,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":6.14544,"Ion.Inject.Time..ms.":22,"RT..min.":16.4023,"First.Scan":16756,"Last.Scan":16756,"Master.Scan.s.":16751,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_05.raw","File.ID":"F23","Quan.Info":"","Peptides.Matched":2590,"XCorr":3.41,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":0.00003,"Percolator.SVMScore":1.725,"ptmRS..Binomial.Peptide.Score":302.93,"ptmRS..Isoform.Confidence.Probability":0.992,"ptmRS..Isoform.Group.Report":"y2+-H(3) O(4) P(14): 811.37","ptmRS..Best.Site.Probabilities":"S13(Phospho): 99.18","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.8; S(13): 99.2; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (N4)","Search.ID":"N","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"APQALMLAPTR","Annotated.Sequence":"[K].APQALmLAPTR.[E]","Modifications":"M6(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6997,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":592.82699,"Contaminant":"False","Aligned.m.z..Da.":592.82702,"MH...Da.":1184.6467,"Theo..MH...Da.":1184.6456,"DeltaM..ppm.":0.92,"Deltam.z..Da.":0.00055,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1221275.625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":22.07225,"Ion.Inject.Time..ms.":22,"RT..min.":10.2204,"First.Scan":9256,"Last.Scan":9256,"Master.Scan.s.":9246,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_05.raw","File.ID":"F23","Quan.Info":"","Peptides.Matched":378,"XCorr":3.13,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":0.00002,"Percolator.SVMScore":1.832,"Precursor.Area":2507685.75,"Apex.RT..min.":10.24,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (N4)","Search.ID":"N","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].msEGITDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S2(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0632,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":731.31511,"Contaminant":"False","Aligned.m.z..Da.":731.31491,"MH...Da.":2191.93079,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":1.35,"Deltam.z..Da.":0.00098,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":278357.21875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":15.5371,"First.Scan":15879,"Last.Scan":15879,"Master.Scan.s.":15867,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_05.raw","File.ID":"F23","Quan.Info":"","Peptides.Matched":2104,"XCorr":3.48,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":0.00007,"Percolator.SVMScore":1.55,"Precursor.Area":557813.875,"Apex.RT..min.":15.55,"ptmRS..Binomial.Peptide.Score":395.46,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(2): 112.04; b1+-H(3) O(4) P(3): 241.08; b1+-H(3) O(4) P(4): 298.10; b1+-H(3) O(4) P(5): 411.19; b1+-H(3) O(4) P(6): 512.24; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+-H(3) O(4) P(9): 869.39; b1+-H(3) O(4) P(10): 998.43; b2+-H(3) O(4) P(10): 499.72","ptmRS..Best.Site.Probabilities":"S2(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 100.0; T(6): 0.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (N4)","Search.ID":"N","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"AGNFALHPEVVR","Annotated.Sequence":"[R].AGNFALHPEVVR.[E]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q03558","Master.Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","Protein.Accessions":"Q03558","Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.5148,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":437.2365,"Contaminant":"False","Aligned.m.z..Da.":437.23652,"MH...Da.":1309.69495,"Theo..MH...Da.":1309.70114,"DeltaM..ppm.":-4.73,"Deltam.z..Da.":-0.00206,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":325077.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":70.90152,"Ion.Inject.Time..ms.":22,"RT..min.":10.1009,"First.Scan":9091,"Last.Scan":9091,"Master.Scan.s.":9090,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_05.raw","File.ID":"F23","Quan.Info":"","Peptides.Matched":493,"XCorr":1.69,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":0.00014,"Percolator.SVMScore":1.411,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (N4)","Search.ID":"N","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].msEGITDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S2(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1513,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1096.46822,"Contaminant":"False","Aligned.m.z..Da.":1096.46792,"MH...Da.":2191.92917,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":0.6,"Deltam.z..Da.":0.00066,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":276593.84375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":15.5039,"First.Scan":15833,"Last.Scan":15833,"Master.Scan.s.":15828,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_05.raw","File.ID":"F23","Quan.Info":"","Peptides.Matched":1989,"XCorr":3.37,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":1.454e-07,"Percolator.SVMScore":2.727,"Precursor.Area":5000513.5,"Apex.RT..min.":15.56,"ptmRS..Binomial.Peptide.Score":481.05,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(2): 112.04; b1+-H(3) O(4) P(3): 241.08; b1+-H(3) O(4) P(4): 298.10; b1+-H(3) O(4) P(5): 411.19; b1+-H(3) O(4) P(6): 512.24; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+-H(3) O(4) P(9): 869.39; b1+-H(3) O(4) P(10): 998.43; b1+(4): 396.08; y1+(14): 1683.77","ptmRS..Best.Site.Probabilities":"S2(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 100.0; T(6): 0.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (N4)","Search.ID":"N","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KDELTLEGIK","Annotated.Sequence":"[K].KDELTLEGIK.[Q]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5066,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":573.32462,"Contaminant":"False","Aligned.m.z..Da.":573.32465,"MH...Da.":1145.64197,"Theo..MH...Da.":1145.64123,"DeltaM..ppm.":0.64,"Deltam.z..Da.":0.00037,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2130915,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":1.20107,"Ion.Inject.Time..ms.":22,"RT..min.":10.3208,"First.Scan":9391,"Last.Scan":9391,"Master.Scan.s.":9389,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_05.raw","File.ID":"F23","Quan.Info":"","Peptides.Matched":302,"XCorr":3.81,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":1.775e-06,"Percolator.SVMScore":2.245,"Precursor.Area":2621553.25,"Apex.RT..min.":10.33,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (N4)","Search.ID":"N","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KDELTLEGIK","Annotated.Sequence":"[K].KDELTLEGIK.[Q]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.603,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":382.55202,"Contaminant":"False","Aligned.m.z..Da.":382.55204,"MH...Da.":1145.64153,"Theo..MH...Da.":1145.64123,"DeltaM..ppm.":0.26,"Deltam.z..Da.":0.0001,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1127167.875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":40.23565,"Ion.Inject.Time..ms.":22,"RT..min.":10.3237,"First.Scan":9396,"Last.Scan":9396,"Master.Scan.s.":9389,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_05.raw","File.ID":"F23","Quan.Info":"","Peptides.Matched":307,"XCorr":2.67,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":0.00142,"Percolator.SVMScore":0.958,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (N4)","Search.ID":"N","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0478,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40758,"Contaminant":"False","Aligned.m.z..Da.":967.40738,"MH...Da.":2900.20821,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":3.06,"Deltam.z..Da.":0.00296,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":472169.28125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":13.12578,"Ion.Inject.Time..ms.":22,"RT..min.":15.358,"First.Scan":15648,"Last.Scan":15648,"Master.Scan.s.":15647,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_05.raw","File.ID":"F23","Quan.Info":"","Peptides.Matched":2633,"XCorr":5.23,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":4.665e-08,"Percolator.SVMScore":2.946,"Precursor.Area":5776421.5,"Apex.RT..min.":14.96,"ptmRS..Binomial.Peptide.Score":374.44,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(14): 1621.73; b1+(12): 1344.56","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (N4)","Search.ID":"N","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DIFSNDELLSDAYDAK","Annotated.Sequence":"[K].DIFSNDELLSDAYDAK.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7325,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":908.41751,"Contaminant":"False","Aligned.m.z..Da.":908.41725,"MH...Da.":1815.82775,"Theo..MH...Da.":1815.82831,"DeltaM..ppm.":-0.31,"Deltam.z..Da.":-0.00028,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":570152.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":11.02786,"Ion.Inject.Time..ms.":22,"RT..min.":17.124,"First.Scan":17391,"Last.Scan":17391,"Master.Scan.s.":17389,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_05.raw","File.ID":"F23","Quan.Info":"","Peptides.Matched":1633,"XCorr":3.14,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":9.434e-10,"Percolator.SVMScore":3.697,"Precursor.Area":1782610.875,"Apex.RT..min.":17.09,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (N4)","Search.ID":"N","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DFRHSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[K].DFRHsDsYSENETNHTNVPISSTGGTNNK.[T]","Modifications":"S5(Phospho); S7(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.1304,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1123.45101,"Contaminant":"False","Aligned.m.z..Da.":1123.45121,"MH...Da.":3368.3385,"Theo..MH...Da.":3368.34888,"DeltaM..ppm.":-3.08,"Deltam.z..Da.":-0.00346,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1742724.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":59.83036,"Ion.Inject.Time..ms.":22,"RT..min.":8.5984,"First.Scan":7063,"Last.Scan":7063,"Master.Scan.s.":7062,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_05.raw","File.ID":"F23","Quan.Info":"","Peptides.Matched":905,"XCorr":4.37,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":8.54e-10,"Percolator.SVMScore":3.716,"Precursor.Area":1127128.875,"Apex.RT..min.":8.59,"ptmRS..Binomial.Peptide.Score":445.48,"ptmRS..Isoform.Confidence.Probability":0.009,"ptmRS..Isoform.Group.Report":"y2+-H(3) O(4) P(22): 1173.53; b1+- -2H(3) O(4) P -2H(3) O(4) P(14): 1646.65; b2+- -2H(3) O(4) P -2H(3) O(4) P(16): 942.88; b2+- -2H(3) O(4) P -2H(3) O(4) P(17): 999.90; b2+- -2H(3) O(4) P -2H(3) O(4) P(18): 1049.44; b2+- -2H(3) O(4) P -2H(3) O(4) P(20): 1154.50; y2+(17): 911.41","ptmRS..Best.Site.Probabilities":"S5(Phospho): 100; T13(Phospho): 98.15","ptmRS..Phospho.Site.Probabilities":"S(5): 100.0; S(7): 0.9; Y(8): 0.0; S(9): 0.9; T(13): 98.2; T(16): 0.0; S(21): 0.0; S(22): 0.0; T(23): 0.0; T(26): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (N4)","Search.ID":"N","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ILISTDLLAR","Annotated.Sequence":"[R].ILISTDLLAR.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5222,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":557.84495,"Contaminant":"False","Aligned.m.z..Da.":557.84476,"MH...Da.":1114.68262,"Theo..MH...Da.":1114.68304,"DeltaM..ppm.":-0.37,"Deltam.z..Da.":-0.00021,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":163704.60938,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":32.26878,"Ion.Inject.Time..ms.":22,"RT..min.":15.7428,"First.Scan":16083,"Last.Scan":16083,"Master.Scan.s.":16082,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_05.raw","File.ID":"F23","Quan.Info":"","Peptides.Matched":124,"XCorr":1.8,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":0.00005,"Percolator.SVMScore":1.59,"Precursor.Area":1647943,"Apex.RT..min.":15.7,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (N4)","Search.ID":"N","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DFRHSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[K].DFRHSDsYsENETNHTNVPISSTGGTNNK.[T]","Modifications":"S7(Phospho); S9(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":1,"Charge":4,"Original.Precursor.Charge":4,"DeltaScore":0.0527,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":842.84064,"Contaminant":"False","Aligned.m.z..Da.":842.8408,"MH...Da.":3368.34076,"Theo..MH...Da.":3368.34888,"DeltaM..ppm.":-2.41,"Deltam.z..Da.":-0.00203,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1743078.375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":2.30246,"Ion.Inject.Time..ms.":22,"RT..min.":8.5825,"First.Scan":7043,"Last.Scan":7043,"Master.Scan.s.":7036,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_05.raw","File.ID":"F23","Quan.Info":"","Peptides.Matched":904,"XCorr":7.02,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":5.179e-10,"Percolator.SVMScore":3.813,"Precursor.Area":2172682.25,"Apex.RT..min.":8.59,"ptmRS..Binomial.Peptide.Score":399.95,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"b2+-H(3) O(4) P(7): 454.16; b2+-H(3) O(4) P(8): 535.69; b2+- -2H(3) O(4) P -2H(3) O(4) P(18): 1049.44; b2+- -2H(3) O(4) P -2H(3) O(4) P(20): 1154.50","ptmRS..Best.Site.Probabilities":"S5(Phospho): 99.98; S7(Phospho): 98.68","ptmRS..Phospho.Site.Probabilities":"S(5): 100.0; S(7): 98.7; Y(8): 0.0; S(9): 1.3; T(13): 0.0; T(16): 0.0; S(21): 0.0; S(22): 0.0; T(23): 0.0; T(26): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (N4)","Search.ID":"N","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DLEQENVEK","Annotated.Sequence":"[K].DLEQENVEK.[E]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.4013,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":552.26455,"Contaminant":"False","Aligned.m.z..Da.":552.2646,"MH...Da.":1103.52182,"Theo..MH...Da.":1103.52151,"DeltaM..ppm.":0.28,"Deltam.z..Da.":0.00016,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1271967.875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":6.4537,"First.Scan":4227,"Last.Scan":4227,"Master.Scan.s.":4215,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_05.raw","File.ID":"F23","Quan.Info":"","Peptides.Matched":557,"XCorr":1.57,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":0.0016,"Percolator.SVMScore":0.935,"Precursor.Area":733165.25,"Apex.RT..min.":6.45,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (N4)","Search.ID":"N","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LQETNPEEVPK","Annotated.Sequence":"[K].LQETNPEEVPK.[F]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":3,"DeltaScore":0.4498,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":642.32799,"Contaminant":"False","Aligned.m.z..Da.":642.32818,"MH...Da.":1283.64871,"Theo..MH...Da.":1283.64777,"DeltaM..ppm.":0.73,"Deltam.z..Da.":0.00047,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":6408487,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":49.54971,"Ion.Inject.Time..ms.":11.107,"RT..min.":7.6266,"First.Scan":5830,"Last.Scan":5830,"Master.Scan.s.":5827,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_05.raw","File.ID":"F23","Quan.Info":"","Peptides.Matched":568,"XCorr":2.29,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":9.453e-06,"Percolator.SVMScore":1.923,"Precursor.Area":6779922,"Apex.RT..min.":7.64,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (N4)","Search.ID":"N","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"IQDPQMTGYVSTR","Annotated.Sequence":"[R].IQDPQmtGYVsTR.[Y]","Modifications":"M6(Oxidation); T7(Phospho); S11(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P32485","Master.Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","Protein.Accessions":"P32485","Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.0576,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":836.32776,"Contaminant":"False","Aligned.m.z..Da.":836.328,"MH...Da.":1671.64824,"Theo..MH...Da.":1671.64853,"DeltaM..ppm.":-0.17,"Deltam.z..Da.":-0.00015,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1978825,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":7.6217,"First.Scan":5826,"Last.Scan":5826,"Master.Scan.s.":5814,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_05.raw","File.ID":"F23","Quan.Info":"","Peptides.Matched":764,"XCorr":2.43,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":0.00031,"Percolator.SVMScore":1.248,"Precursor.Area":2103129.75,"Apex.RT..min.":7.63,"ptmRS..Binomial.Peptide.Score":334.77,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(11): 1332.53; y1+-H(3) O(4) P(10): 1217.50; y1+-H(3) O(4) P(8): 992.39; y1+-H(3) O(4) P(7): 845.36; y2+-H(3) O(4) P(11): 666.77; y2+-H(3) O(4) P(10): 609.25; y1+(4): 462.27; y1+(3): 363.20; y1+(2): 276.17","ptmRS..Best.Site.Probabilities":"T7(Phospho): 100; Y9(Phospho): 50; T12(Phospho): 50","ptmRS..Phospho.Site.Probabilities":"T(7): 100.0; Y(9): 50.0; S(11): 0.0; T(12): 50.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (N4)","Search.ID":"N","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LQETNPEEVPK","Annotated.Sequence":"[K].LQETNPEEVPK.[F]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5342,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":642.32812,"Contaminant":"False","Aligned.m.z..Da.":642.32831,"MH...Da.":1283.64896,"Theo..MH...Da.":1283.64777,"DeltaM..ppm.":0.93,"Deltam.z..Da.":0.0006,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3746665.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":31.74896,"Ion.Inject.Time..ms.":22,"RT..min.":7.6171,"First.Scan":5819,"Last.Scan":5819,"Master.Scan.s.":5814,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_05.raw","File.ID":"F23","Quan.Info":"","Peptides.Matched":516,"XCorr":2.34,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":4.914e-06,"Percolator.SVMScore":2.049,"Precursor.Area":6779922,"Apex.RT..min.":7.64,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (N4)","Search.ID":"N","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"IGNNELLHR","Annotated.Sequence":"[K].IGNNELLHR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q03558","Master.Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","Protein.Accessions":"Q03558","Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.6598,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":355.8648,"Contaminant":"False","Aligned.m.z..Da.":355.8649,"MH...Da.":1065.57985,"Theo..MH...Da.":1065.57997,"DeltaM..ppm.":-0.11,"Deltam.z..Da.":-0.00004,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2097542.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":12.35518,"Ion.Inject.Time..ms.":22,"RT..min.":7.6523,"First.Scan":5862,"Last.Scan":5862,"Master.Scan.s.":5853,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_05.raw","File.ID":"F23","Quan.Info":"","Peptides.Matched":300,"XCorr":2.41,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":0.00005,"Percolator.SVMScore":1.615,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (N4)","Search.ID":"N","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MGSINNNFNTNNNSNTDLDRDWK","Annotated.Sequence":"[-].mGSINNNFNTNNNsNTDLDRDWK.[T]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S14(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P39517","Master.Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","Protein.Accessions":"P39517","Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0532,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":892.37445,"Contaminant":"False","Aligned.m.z..Da.":892.37441,"MH...Da.":2675.10881,"Theo..MH...Da.":2675.11179,"DeltaM..ppm.":-1.11,"Deltam.z..Da.":-0.00099,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":137199.875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":19.79097,"Ion.Inject.Time..ms.":22,"RT..min.":13.4305,"First.Scan":13563,"Last.Scan":13563,"Master.Scan.s.":13556,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_05.raw","File.ID":"F23","Quan.Info":"","Peptides.Matched":2080,"XCorr":3.01,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":0.00002,"Percolator.SVMScore":1.795,"Precursor.Area":1085329.125,"Apex.RT..min.":13.46,"ptmRS..Binomial.Peptide.Score":351.57,"ptmRS..Isoform.Confidence.Probability":0.992,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(11): 1345.61; y1+-H(3) O(4) P(10): 1231.57; b2+-H(3) O(4) P(21): 1122.98; y2+-H(3) O(4) P(15): 894.90; y2+-H(3) O(4) P(12): 730.33; y2+-H(3) O(4) P(10): 616.29; y1+(9): 1162.55","ptmRS..Best.Site.Probabilities":"S14(Phospho): 99.19","ptmRS..Phospho.Site.Probabilities":"S(3): 0.0; T(10): 0.0; S(14): 99.2; T(16): 0.8"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (N4)","Search.ID":"N","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"TGTFSIAALQR","Annotated.Sequence":"[K].TGTFSIAALQR.[I]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7769,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":582.82247,"Contaminant":"False","Aligned.m.z..Da.":582.82244,"MH...Da.":1164.63766,"Theo..MH...Da.":1164.63715,"DeltaM..ppm.":0.44,"Deltam.z..Da.":0.00026,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":648071.6875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":26.77173,"Ion.Inject.Time..ms.":22,"RT..min.":13.4823,"First.Scan":13604,"Last.Scan":13604,"Master.Scan.s.":13596,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_05.raw","File.ID":"F23","Quan.Info":"","Peptides.Matched":362,"XCorr":2.6,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":5.157e-06,"Percolator.SVMScore":2.04,"Precursor.Area":412824.09375,"Apex.RT..min.":13.47,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (N4)","Search.ID":"N","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"HSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[R].HSDSySENETNHTNVPISSTGGTNNK.[T]","Modifications":"Y5(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":0,"Charge":4,"Original.Precursor.Charge":3,"DeltaScore":0.0089,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":718.30244,"Contaminant":"False","Aligned.m.z..Da.":718.30264,"MH...Da.":2870.18794,"Theo..MH...Da.":2870.18608,"DeltaM..ppm.":0.65,"Deltam.z..Da.":0.00046,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3867954.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":34.63847,"Ion.Inject.Time..ms.":22,"RT..min.":7.4795,"First.Scan":5657,"Last.Scan":5657,"Master.Scan.s.":5645,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_05.raw","File.ID":"F23","Quan.Info":"","Peptides.Matched":2519,"XCorr":3.38,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":0.00002,"Percolator.SVMScore":1.763,"Precursor.Area":4459637,"Apex.RT..min.":7.48,"ptmRS..Binomial.Peptide.Score":189.15,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(11): 1246.46; b2+-H(3) O(4) P(14): 799.81","ptmRS..Best.Site.Probabilities":"S6(Phospho): 49.36; T10(Phospho): 49.36","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(4): 0.6; Y(5): 0.0; S(6): 49.4; T(10): 49.4; T(13): 0.6; S(18): 0.0; S(19): 0.0; T(20): 0.0; T(23): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (N4)","Search.ID":"N","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"IQDPQMTGYVSTR","Annotated.Sequence":"[R].IQDPQmTGyVSTR.[Y]","Modifications":"M6(Oxidation); Y9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P32485","Master.Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","Protein.Accessions":"P32485","Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1225,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":796.34474,"Contaminant":"False","Aligned.m.z..Da.":796.34497,"MH...Da.":1591.68221,"Theo..MH...Da.":1591.6822,"DeltaM..ppm.":0,"Deltam.z..Da.":0,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3157005,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":7.5539,"First.Scan":5745,"Last.Scan":5745,"Master.Scan.s.":5736,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_05.raw","File.ID":"F23","Quan.Info":"","Peptides.Matched":1251,"XCorr":3.02,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":1.135e-06,"Percolator.SVMScore":2.331,"Precursor.Area":6031521.5,"Apex.RT..min.":7.59,"ptmRS..Binomial.Peptide.Score":265.8,"ptmRS..Isoform.Confidence.Probability":0.333,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(11): 1252.56; y1+-H(3) O(4) P(10): 1137.54","ptmRS..Best.Site.Probabilities":"T7(Phospho): 33.28; Y9(Phospho): 33.28; S11(Phospho): 33.28","ptmRS..Phospho.Site.Probabilities":"T(7): 33.3; Y(9): 33.3; S(11): 33.3; T(12): 0.2"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (N4)","Search.ID":"N","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"HSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[R].HSDSySENETNHTNVPISSTGGTNNK.[T]","Modifications":"Y5(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":0,"Charge":4,"Original.Precursor.Charge":4,"DeltaScore":0.0054,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":718.30244,"Contaminant":"False","Aligned.m.z..Da.":718.30264,"MH...Da.":2870.18795,"Theo..MH...Da.":2870.18608,"DeltaM..ppm.":0.65,"Deltam.z..Da.":0.00047,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3867954.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":7.4741,"First.Scan":5649,"Last.Scan":5649,"Master.Scan.s.":5645,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_05.raw","File.ID":"F23","Quan.Info":"","Peptides.Matched":2518,"XCorr":5.57,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":4.785e-10,"Percolator.SVMScore":3.828,"Precursor.Area":4459637,"Apex.RT..min.":7.48,"ptmRS..Binomial.Peptide.Score":302.19,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(9): 1031.37; b1+-H(3) O(4) P(10): 1132.42; b1+-H(3) O(4) P(11): 1246.46; b1+-H(3) O(4) P(12): 1383.52; b2+-H(3) O(4) P(7): 394.65; b2+-H(3) O(4) P(13): 742.79; b2+-H(3) O(4) P(14): 799.81; b2+-H(3) O(4) P(15): 849.34; b2+-H(3) O(4) P(17): 954.41; b1+(4): 427.16","ptmRS..Best.Site.Probabilities":"S6(Phospho): 99.36","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(4): 0.6; Y(5): 0.0; S(6): 99.4; T(10): 0.0; T(13): 0.0; S(18): 0.0; S(19): 0.0; T(20): 0.0; T(23): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (N4)","Search.ID":"N","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"HSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[R].HSDSYsENETNHTNVPISSTGGTNNK.[T]","Modifications":"S6(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0093,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":957.39925,"Contaminant":"False","Aligned.m.z..Da.":957.39952,"MH...Da.":2870.18322,"Theo..MH...Da.":2870.18608,"DeltaM..ppm.":-1,"Deltam.z..Da.":-0.00095,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2183606,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":7.4623,"First.Scan":5635,"Last.Scan":5635,"Master.Scan.s.":5632,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_05.raw","File.ID":"F23","Quan.Info":"","Peptides.Matched":2388,"XCorr":6.44,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":5.684e-14,"Percolator.SVMScore":5.568,"Precursor.Area":9008282,"Apex.RT..min.":7.48,"ptmRS..Binomial.Peptide.Score":568.08,"ptmRS..Isoform.Confidence.Probability":0.006,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(4): 409.15; b1+-H(3) O(4) P(5): 572.21; b1+-H(3) O(4) P(6): 659.24; b1+-H(3) O(4) P(9): 1031.37; b1+-H(3) O(4) P(10): 1132.42; b1+-H(3) O(4) P(11): 1246.46; b1+-H(3) O(4) P(12): 1383.52; b1+-H(3) O(4) P(14): 1598.61; b1+-H(3) O(4) P(15): 1697.68; b2+-H(3) O(4) P(12): 692.26; b2+-H(3) O(4) P(13): 742.79; b2+-H(3) O(4) P(14): 799.81; b2+-H(3) O(4) P(15): 849.34; y2+-H(3) O(4) P(25): 1318.08; y2+-H(3) O(4) P(24): 1274.56; y2+-H(3) O(4) P(23): 1217.05","ptmRS..Best.Site.Probabilities":"S4(Phospho): 99.36","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(4): 99.4; Y(5): 0.0; S(6): 0.6; T(10): 0.0; T(13): 0.0; S(18): 0.0; S(19): 0.0; T(20): 0.0; T(23): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (N4)","Search.ID":"N","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KSDEPSRESTPVR","Annotated.Sequence":"[K].KSDEPsREsTPVR.[S]","Modifications":"S6(Phospho); S9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q08421","Master.Protein.Descriptions":"Enhancer of translation termination 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ETT1 PE=1 SV=1","Protein.Accessions":"Q08421","Protein.Descriptions":"Enhancer of translation termination 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ETT1 PE=1 SV=1","X..Missed.Cleavages":2,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1352,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":824.34262,"Contaminant":"False","Aligned.m.z..Da.":824.34254,"MH...Da.":1647.67796,"Theo..MH...Da.":1647.67752,"DeltaM..ppm.":0.26,"Deltam.z..Da.":0.00022,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":13318768,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":10.439,"RT..min.":4.5194,"First.Scan":1361,"Last.Scan":1361,"Master.Scan.s.":1355,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_05.raw","File.ID":"F23","Quan.Info":"","Peptides.Matched":986,"XCorr":3.18,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":0.00008,"Percolator.SVMScore":1.515,"Precursor.Area":7116509,"Apex.RT..min.":4.52,"ptmRS..Binomial.Peptide.Score":392.69,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(9): 998.45; y1+-H(3) O(4) P(10): 1219.55; y1+-H(3) O(4) P(9): 1090.50; y1+-H(3) O(4) P(5): 541.31; y1+-H(3) O(4) P(4): 454.28; y2+-H(3) O(4) P(10): 610.28; y2+-H(3) O(4) P(9): 545.76; b1+- -2H(3) O(4) P -2H(3) O(4) P(10): 1081.49; b1+- -2H(3) O(4) P -2H(3) O(4) P(12): 1277.61","ptmRS..Best.Site.Probabilities":"S6(Phospho): 100; T10(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(6): 100.0; S(9): 0.0; T(10): 100.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (N4)","Search.ID":"N","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"AAEAGETGAATSATEGDNNNNTAAGDKK","Annotated.Sequence":"[K].AAEAGETGAATsATEGDNNNNTAAGDKK.[G]","Modifications":"S12(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0345,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":906.04965,"Contaminant":"False","Aligned.m.z..Da.":906.04971,"MH...Da.":2716.1344,"Theo..MH...Da.":2716.13298,"DeltaM..ppm.":0.52,"Deltam.z..Da.":0.00047,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3731233.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":1.4332,"Ion.Inject.Time..ms.":22,"RT..min.":5.0398,"First.Scan":1959,"Last.Scan":1959,"Master.Scan.s.":1953,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_05.raw","File.ID":"F23","Quan.Info":"","Peptides.Matched":2540,"XCorr":6.96,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":5.691e-16,"Percolator.SVMScore":7.814,"Precursor.Area":3223340.75,"Apex.RT..min.":5.04,"ptmRS..Binomial.Peptide.Score":509.93,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(18): 1789.79; y1+-H(3) O(4) P(17): 1688.75; y2+-H(3) O(4) P(22): 1045.47; y2+-H(3) O(4) P(21): 994.95; y2+-H(3) O(4) P(18): 895.40; y2+-H(3) O(4) P(17): 844.88","ptmRS..Best.Site.Probabilities":"S12(Phospho): 99.99","ptmRS..Phospho.Site.Probabilities":"T(7): 0.0; T(11): 0.0; S(12): 100.0; T(14): 0.0; T(22): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (N4)","Search.ID":"N","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"AAEAGETGAATSATEGDNNNNTAAGDK","Annotated.Sequence":"[K].AAEAGETGAATsATEGDNNNNTAAGDK.[K]","Modifications":"S12(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0047,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":863.35118,"Contaminant":"False","Aligned.m.z..Da.":863.35124,"MH...Da.":2588.03898,"Theo..MH...Da.":2588.03802,"DeltaM..ppm.":0.37,"Deltam.z..Da.":0.00032,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1779625.125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":5.7503,"First.Scan":2963,"Last.Scan":2963,"Master.Scan.s.":2954,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_05.raw","File.ID":"F23","Quan.Info":"","Peptides.Matched":1498,"XCorr":4.29,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":7.694e-09,"Percolator.SVMScore":3.293,"Precursor.Area":1117985.75,"Apex.RT..min.":5.74,"ptmRS..Binomial.Peptide.Score":316.48,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(7): 612.26; b1+-H(3) O(4) P(9): 740.32","ptmRS..Best.Site.Probabilities":"T7(Phospho): 99.99","ptmRS..Phospho.Site.Probabilities":"T(7): 100.0; T(11): 0.0; S(12): 0.0; T(14): 0.0; T(22): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (N4)","Search.ID":"N","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"AIMPIIEGHDVLAQAQSGTGK","Annotated.Sequence":"[R].AImPIIEGHDVLAQAQSGTGK.[T]","Modifications":"M3(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.6276,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":718.03834,"Contaminant":"False","Aligned.m.z..Da.":718.03821,"MH...Da.":2152.10048,"Theo..MH...Da.":2152.10668,"DeltaM..ppm.":-2.88,"Deltam.z..Da.":-0.00207,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":828312.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":7.5601,"Ion.Inject.Time..ms.":22,"RT..min.":12.6655,"First.Scan":12601,"Last.Scan":12601,"Master.Scan.s.":12592,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_05.raw","File.ID":"F23","Quan.Info":"","Peptides.Matched":1111,"XCorr":3.84,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":1.471e-08,"Percolator.SVMScore":3.168,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (N4)","Search.ID":"N","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"HSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[R].HSDsYsENETNHTNVPISSTGGTNNK.[T]","Modifications":"S4(Phospho); S6(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.1476,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":984.05544,"Contaminant":"False","Aligned.m.z..Da.":984.05573,"MH...Da.":2950.15177,"Theo..MH...Da.":2950.15241,"DeltaM..ppm.":-0.22,"Deltam.z..Da.":-0.00021,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":4630321.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":7.9235,"First.Scan":6193,"Last.Scan":6193,"Master.Scan.s.":6191,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_05.raw","File.ID":"F23","Quan.Info":"","Peptides.Matched":1103,"XCorr":6.91,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":4.913e-12,"Percolator.SVMScore":4.709,"Precursor.Area":4318936,"Apex.RT..min.":7.93,"ptmRS..Binomial.Peptide.Score":637.44,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(6): 739.21; b1+-H(3) O(4) P(9): 1111.34; y2+-H(3) O(4) P(23): 1257.03; y2+-H(3) O(4) P(22): 1173.53; y2+-H(3) O(4) P(21): 1092.00; b1+- -2H(3) O(4) P -2H(3) O(4) P(6): 641.23; b1+- -2H(3) O(4) P -2H(3) O(4) P(11): 1228.45; b2+- -2H(3) O(4) P -2H(3) O(4) P(12): 683.26; b2+- -2H(3) O(4) P -2H(3) O(4) P(14): 790.80; b2+- -2H(3) O(4) P -2H(3) O(4) P(15): 840.34","ptmRS..Best.Site.Probabilities":"S4(Phospho): 100; S6(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(4): 100.0; Y(5): 0.0; S(6): 100.0; T(10): 0.0; T(13): 0.0; S(18): 0.0; S(19): 0.0; T(20): 0.0; T(23): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (N4)","Search.ID":"N","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSSPLHRLSPTGK","Annotated.Sequence":"[R].mSsPLHRLsPTGK.[S]","Modifications":"M1(Oxidation); S3(Phospho); S9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.4779,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":793.84341,"Contaminant":"False","Aligned.m.z..Da.":793.84365,"MH...Da.":1586.67956,"Theo..MH...Da.":1586.67977,"DeltaM..ppm.":-0.13,"Deltam.z..Da.":-0.00011,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2820089.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":7.7077,"First.Scan":5929,"Last.Scan":5929,"Master.Scan.s.":5918,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_05.raw","File.ID":"F23","Quan.Info":"","Peptides.Matched":988,"XCorr":2.49,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":0.00009,"Percolator.SVMScore":1.482,"Precursor.Area":2279547.25,"Apex.RT..min.":7.72,"ptmRS..Binomial.Peptide.Score":307.7,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(3): 304.10; b1+-H(3) O(4) P(8): 920.48; y2+-H(3) O(4) P(11): 627.82; y2+- -2H(3) O(4) P -2H(3) O(4) P(11): 578.83; b1+(2): 235.07","ptmRS..Best.Site.Probabilities":"S3(Phospho): 100; S9(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(3): 100.0; S(9): 100.0; T(11): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (N4)","Search.ID":"N","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DFLLKPELSR","Annotated.Sequence":"[K].DFLLKPELSR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.6208,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":406.56731,"Contaminant":"False","Aligned.m.z..Da.":406.56728,"MH...Da.":1217.68739,"Theo..MH...Da.":1217.68885,"DeltaM..ppm.":-1.2,"Deltam.z..Da.":-0.00049,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":571228.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":8.50901,"Ion.Inject.Time..ms.":22,"RT..min.":13.243,"First.Scan":13356,"Last.Scan":13356,"Master.Scan.s.":13349,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_05.raw","File.ID":"F23","Quan.Info":"","Peptides.Matched":283,"XCorr":2.4,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":0.00004,"Percolator.SVMScore":1.652,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (N4)","Search.ID":"N","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSSPLHRLSPTGK","Annotated.Sequence":"[R].mSsPLHRLsPTGK.[S]","Modifications":"M1(Oxidation); S3(Phospho); S9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.4154,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":529.56511,"Contaminant":"False","Aligned.m.z..Da.":529.56526,"MH...Da.":1586.68077,"Theo..MH...Da.":1586.67977,"DeltaM..ppm.":0.63,"Deltam.z..Da.":0.00033,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2851122.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":44.48354,"Ion.Inject.Time..ms.":22,"RT..min.":7.695,"First.Scan":5913,"Last.Scan":5913,"Master.Scan.s.":5905,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_05.raw","File.ID":"F23","Quan.Info":"","Peptides.Matched":1087,"XCorr":3.25,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":0.00007,"Percolator.SVMScore":1.532,"Precursor.Area":5407123.5,"Apex.RT..min.":7.72,"ptmRS..Binomial.Peptide.Score":278.87,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(8): 877.50; y2+-H(3) O(4) P(12): 671.34; y2+-H(3) O(4) P(11): 627.82; y2+-H(3) O(4) P(10): 544.32; y2+-H(3) O(4) P(8): 439.25; y1+(4): 402.23; y1+(3): 305.18","ptmRS..Best.Site.Probabilities":"S3(Phospho): 100; S9(Phospho): 99.99","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(3): 100.0; S(9): 100.0; T(11): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AF4)","Search.ID":"AF","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].msEGITDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S2(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1623,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1096.46707,"Contaminant":"False","Aligned.m.z..Da.":1096.467,"MH...Da.":2191.92686,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":-0.45,"Deltam.z..Da.":-0.00049,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":489595.03125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":15.7576,"First.Scan":15573,"Last.Scan":15573,"Master.Scan.s.":15571,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y25_05.raw","File.ID":"F5","Quan.Info":"","Peptides.Matched":1964,"XCorr":4.62,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":1.859e-09,"Percolator.SVMScore":3.595,"Precursor.Area":2565022.25,"Apex.RT..min.":15.79,"ptmRS..Binomial.Peptide.Score":530.63,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(2): 112.04; b1+-H(3) O(4) P(3): 241.08; b1+-H(3) O(4) P(4): 298.10; b1+-H(3) O(4) P(5): 411.19; b1+-H(3) O(4) P(6): 512.24; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+-H(3) O(4) P(9): 869.39; b1+-H(3) O(4) P(10): 998.43; b1+-H(3) O(4) P(13): 1326.61; b1+(4): 396.08; y1+(14): 1683.77","ptmRS..Best.Site.Probabilities":"S2(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 100.0; T(6): 0.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AF4)","Search.ID":"AF","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"TGTFSIAALQR","Annotated.Sequence":"[K].TGTFSIAALQR.[I]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7355,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":582.82262,"Contaminant":"False","Aligned.m.z..Da.":582.82267,"MH...Da.":1164.63797,"Theo..MH...Da.":1164.63715,"DeltaM..ppm.":0.71,"Deltam.z..Da.":0.00041,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":368219.5625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":31.70618,"Ion.Inject.Time..ms.":22,"RT..min.":13.3754,"First.Scan":12782,"Last.Scan":12782,"Master.Scan.s.":12778,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y25_05.raw","File.ID":"F5","Quan.Info":"","Peptides.Matched":360,"XCorr":2.76,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":1.695e-06,"Percolator.SVMScore":2.291,"Precursor.Area":294614.21875,"Apex.RT..min.":13.38,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AF4)","Search.ID":"AF","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].msEGITDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S2(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0774,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":731.31282,"Contaminant":"False","Aligned.m.z..Da.":731.31277,"MH...Da.":2191.92392,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":-1.79,"Deltam.z..Da.":-0.00131,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":477530.5625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":8.45185,"Ion.Inject.Time..ms.":22,"RT..min.":15.7691,"First.Scan":15588,"Last.Scan":15588,"Master.Scan.s.":15584,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y25_05.raw","File.ID":"F5","Quan.Info":"","Peptides.Matched":1847,"XCorr":3.1,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":0.00007,"Percolator.SVMScore":1.57,"Precursor.Area":493596.53125,"Apex.RT..min.":15.79,"ptmRS..Binomial.Peptide.Score":329.37,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(2): 112.04; b1+-H(3) O(4) P(3): 241.08; b1+-H(3) O(4) P(4): 298.10; b1+-H(3) O(4) P(5): 411.19; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+-H(3) O(4) P(9): 869.39; b1+-H(3) O(4) P(10): 998.43","ptmRS..Best.Site.Probabilities":"S2(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 100.0; T(6): 0.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AF4)","Search.ID":"AF","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"GVFGYGFEEPSAIQQR","Annotated.Sequence":"[R].GVFGYGFEEPSAIQQR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5058,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":892.93578,"Contaminant":"False","Aligned.m.z..Da.":892.93571,"MH...Da.":1784.86429,"Theo..MH...Da.":1784.86022,"DeltaM..ppm.":2.28,"Deltam.z..Da.":0.00203,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":174151.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":17.3312,"Ion.Inject.Time..ms.":22,"RT..min.":15.0336,"First.Scan":14718,"Last.Scan":14718,"Master.Scan.s.":14716,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y25_05.raw","File.ID":"F5","Quan.Info":"","Peptides.Matched":1492,"XCorr":1.72,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":0.0008,"Percolator.SVMScore":1.114,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AF4)","Search.ID":"AF","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":2,"DeltaScore":0.0026,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40827,"Contaminant":"False","Aligned.m.z..Da.":967.4082,"MH...Da.":2900.21026,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":3.77,"Deltam.z..Da.":0.00365,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":257762.40625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":49.28204,"Ion.Inject.Time..ms.":22,"RT..min.":15.3357,"First.Scan":15093,"Last.Scan":15093,"Master.Scan.s.":15082,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y25_05.raw","File.ID":"F5","Quan.Info":"","Peptides.Matched":2662,"XCorr":3.88,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":7.625e-08,"Percolator.SVMScore":2.884,"ptmRS..Binomial.Peptide.Score":304.82,"ptmRS..Isoform.Confidence.Probability":0.5,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"Y12(Phospho): 50; S13(Phospho): 50","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 50.0; S(13): 50.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AF4)","Search.ID":"AF","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEySDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); Y12(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.4099,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40709,"Contaminant":"False","Aligned.m.z..Da.":967.40701,"MH...Da.":2900.20672,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":2.55,"Deltam.z..Da.":0.00246,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":275360.40625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":52.74048,"Ion.Inject.Time..ms.":22,"RT..min.":15.0483,"First.Scan":14738,"Last.Scan":14738,"Master.Scan.s.":14729,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y25_05.raw","File.ID":"F5","Quan.Info":"","Peptides.Matched":2599,"XCorr":3.83,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":6.901e-07,"Percolator.SVMScore":2.463,"Precursor.Area":4454748.5,"Apex.RT..min.":15.09,"ptmRS..Binomial.Peptide.Score":323.07,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"y2+-H(3) O(4) P(14): 811.37; b1+(12): 1344.56","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AF4)","Search.ID":"AF","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":5,"DeltaScore":0.0725,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40429,"Contaminant":"False","Aligned.m.z..Da.":967.40421,"MH...Da.":2900.19831,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":-0.35,"Deltam.z..Da.":-0.00034,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2421550,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":9.05842,"Ion.Inject.Time..ms.":22,"RT..min.":15.1238,"First.Scan":14831,"Last.Scan":14831,"Master.Scan.s.":14823,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y25_05.raw","File.ID":"F5","Quan.Info":"","Peptides.Matched":2428,"XCorr":6.62,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":6.591e-15,"Percolator.SVMScore":5.995,"Precursor.Area":4454748.5,"Apex.RT..min.":15.09,"ptmRS..Binomial.Peptide.Score":656.03,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(13): 1413.58; b1+-H(3) O(4) P(15): 1642.65; y1+-H(3) O(4) P(14): 1621.73; y1+-H(3) O(4) P(13): 1458.67; b2+-H(3) O(4) P(16): 886.35; b2+-H(3) O(4) P(17): 950.38; b2+-H(3) O(4) P(18): 1014.90; y2+-H(3) O(4) P(14): 811.37; b1+(12): 1344.56; y1+(13): 1556.65","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AF4)","Search.ID":"AF","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MGSINNNFNTNNNSNTDLDRDWK","Annotated.Sequence":"[-].mGSINNNFNTNNNsNTDLDRDWK.[T]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S14(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P39517","Master.Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","Protein.Accessions":"P39517","Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0453,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":892.37258,"Contaminant":"False","Aligned.m.z..Da.":892.37262,"MH...Da.":2675.1032,"Theo..MH...Da.":2675.11179,"DeltaM..ppm.":-3.21,"Deltam.z..Da.":-0.00286,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":122862.83594,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":20.64987,"Ion.Inject.Time..ms.":22,"RT..min.":13.5483,"First.Scan":13002,"Last.Scan":13002,"Master.Scan.s.":12997,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y25_05.raw","File.ID":"F5","Quan.Info":"","Peptides.Matched":2077,"XCorr":2.65,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":0.00016,"Percolator.SVMScore":1.422,"Precursor.Area":625804.375,"Apex.RT..min.":13.57,"ptmRS..Binomial.Peptide.Score":293.47,"ptmRS..Isoform.Confidence.Probability":0.991,"ptmRS..Isoform.Group.Report":"y2+-H(3) O(4) P(16): 968.43; y2+-H(3) O(4) P(15): 894.90; y2+-H(3) O(4) P(14): 837.88; y2+-H(3) O(4) P(12): 730.33","ptmRS..Best.Site.Probabilities":"S14(Phospho): 99.07","ptmRS..Phospho.Site.Probabilities":"S(3): 0.0; T(10): 0.9; S(14): 99.1; T(16): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AF4)","Search.ID":"AF","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0029,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40278,"Contaminant":"False","Aligned.m.z..Da.":967.40271,"MH...Da.":2900.1938,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":-1.91,"Deltam.z..Da.":-0.00184,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":177238.65625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":9.90168,"Ion.Inject.Time..ms.":22,"RT..min.":15.5643,"First.Scan":15355,"Last.Scan":15355,"Master.Scan.s.":15351,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y25_05.raw","File.ID":"F5","Quan.Info":"","Peptides.Matched":2231,"XCorr":3.41,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":4.545e-06,"Percolator.SVMScore":2.102,"Precursor.Area":4454748.5,"Apex.RT..min.":15.09,"ptmRS..Binomial.Peptide.Score":301.73,"ptmRS..Isoform.Confidence.Probability":0.5,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"Y12(Phospho): 50; S13(Phospho): 50","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 50.0; S(13): 50.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AF4)","Search.ID":"AF","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSSPLHRLSPTGK","Annotated.Sequence":"[R].mSsPLHRLsPTGK.[S]","Modifications":"M1(Oxidation); S3(Phospho); S9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.4209,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":529.56523,"Contaminant":"False","Aligned.m.z..Da.":529.5653,"MH...Da.":1586.68114,"Theo..MH...Da.":1586.67977,"DeltaM..ppm.":0.87,"Deltam.z..Da.":0.00046,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1295341.625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":11.04927,"Ion.Inject.Time..ms.":22,"RT..min.":7.7406,"First.Scan":5485,"Last.Scan":5485,"Master.Scan.s.":5476,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y25_05.raw","File.ID":"F5","Quan.Info":"","Peptides.Matched":1121,"XCorr":3.16,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":0.00018,"Percolator.SVMScore":1.397,"ptmRS..Binomial.Peptide.Score":209.52,"ptmRS..Isoform.Confidence.Probability":0.99,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(8): 877.50; y2+-H(3) O(4) P(11): 627.82; y2+-H(3) O(4) P(10): 544.32; y2+-H(3) O(4) P(9): 495.80; y2+-H(3) O(4) P(8): 439.25; y1+(4): 402.23","ptmRS..Best.Site.Probabilities":"S3(Phospho): 100; S9(Phospho): 99","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(3): 100.0; S(9): 99.0; T(11): 1.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AF4)","Search.ID":"AF","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"HSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[R].HSDSySENETNHTNVPISSTGGTNNK.[T]","Modifications":"Y5(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0178,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":957.39974,"Contaminant":"False","Aligned.m.z..Da.":957.39986,"MH...Da.":2870.18466,"Theo..MH...Da.":2870.18608,"DeltaM..ppm.":-0.49,"Deltam.z..Da.":-0.00047,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":861537.125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":7.6443,"First.Scan":5343,"Last.Scan":5343,"Master.Scan.s.":5333,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y25_05.raw","File.ID":"F5","Quan.Info":"","Peptides.Matched":2478,"XCorr":5.61,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":1.727e-12,"Percolator.SVMScore":4.93,"ptmRS..Binomial.Peptide.Score":267.54,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(4): 409.15; b1+-H(3) O(4) P(5): 572.21; b1+-H(3) O(4) P(6): 659.24; b1+-H(3) O(4) P(9): 1031.37; b1+-H(3) O(4) P(11): 1246.46; b1+-H(3) O(4) P(15): 1697.68; b2+-H(3) O(4) P(12): 692.26; b2+-H(3) O(4) P(13): 742.79; b2+-H(3) O(4) P(14): 799.81; b2+-H(3) O(4) P(15): 849.34; b2+-H(3) O(4) P(20): 1091.97; y2+-H(3) O(4) P(25): 1318.08; y2+-H(3) O(4) P(24): 1274.56; y2+-H(3) O(4) P(23): 1217.05","ptmRS..Best.Site.Probabilities":"S4(Phospho): 50; S6(Phospho): 50","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(4): 50.0; Y(5): 0.0; S(6): 50.0; T(10): 0.0; T(13): 0.0; S(18): 0.0; S(19): 0.0; T(20): 0.0; T(23): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AF4)","Search.ID":"AF","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"HSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[R].HSDSySENETNHTNVPISSTGGTNNK.[T]","Modifications":"Y5(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":0,"Charge":4,"Original.Precursor.Charge":4,"DeltaScore":0.0055,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":718.30175,"Contaminant":"False","Aligned.m.z..Da.":718.30184,"MH...Da.":2870.18519,"Theo..MH...Da.":2870.18608,"DeltaM..ppm.":-0.31,"Deltam.z..Da.":-0.00022,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1021105.4375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":23.00777,"Ion.Inject.Time..ms.":22,"RT..min.":7.6514,"First.Scan":5353,"Last.Scan":5353,"Master.Scan.s.":5346,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y25_05.raw","File.ID":"F5","Quan.Info":"","Peptides.Matched":2489,"XCorr":5.45,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":8.378e-09,"Percolator.SVMScore":3.307,"ptmRS..Binomial.Peptide.Score":252.6,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"b2+-H(3) O(4) P(7): 394.65; b2+-H(3) O(4) P(13): 742.79; b2+-H(3) O(4) P(14): 799.81; b2+-H(3) O(4) P(15): 849.34; b2+-H(3) O(4) P(17): 954.41; b1+(4): 427.16","ptmRS..Best.Site.Probabilities":"S6(Phospho): 97.14","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(4): 1.0; Y(5): 0.0; S(6): 97.1; T(10): 1.0; T(13): 1.0; S(18): 0.0; S(19): 0.0; T(20): 0.0; T(23): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AF4)","Search.ID":"AF","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"IQDPQMTGYVSTR","Annotated.Sequence":"[R].IQDPQMTGyVSTR.[Y]","Modifications":"Y9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P32485","Master.Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","Protein.Accessions":"P32485","Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1012,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":788.34426,"Contaminant":"False","Aligned.m.z..Da.":788.34423,"MH...Da.":1575.68124,"Theo..MH...Da.":1575.68728,"DeltaM..ppm.":-3.84,"Deltam.z..Da.":-0.00302,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1239746.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":30.02696,"Ion.Inject.Time..ms.":22,"RT..min.":9.5905,"First.Scan":7880,"Last.Scan":7880,"Master.Scan.s.":7868,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y25_05.raw","File.ID":"F5","Quan.Info":"","Peptides.Matched":1130,"XCorr":2.57,"X..Protein.Groups":1,"Percolator.q.Value":0.00015,"Percolator.PEP":0.00014,"Percolator.SVMScore":1.45,"ptmRS..Binomial.Peptide.Score":247.57,"ptmRS..Isoform.Confidence.Probability":0.985,"ptmRS..Isoform.Group.Report":"y1+(6): 762.32; y1+(5): 705.30","ptmRS..Best.Site.Probabilities":"Y9(Phospho): 98.53","ptmRS..Phospho.Site.Probabilities":"T(7): 0.5; Y(9): 98.5; S(11): 0.5; T(12): 0.5"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (G4)","Search.ID":"G","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"TAVFYGGTPISK","Annotated.Sequence":"[K].TAVFYGGTPISK.[D]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.622,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":620.83286,"Contaminant":"False","Aligned.m.z..Da.":620.8328,"MH...Da.":1240.65846,"Theo..MH...Da.":1240.65721,"DeltaM..ppm.":1,"Deltam.z..Da.":0.00062,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1390412.625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":36.3213,"Ion.Inject.Time..ms.":22,"RT..min.":11.1765,"First.Scan":10532,"Last.Scan":10532,"Master.Scan.s.":10524,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_06.raw","File.ID":"F30","Quan.Info":"","Peptides.Matched":479,"XCorr":2.46,"X..Protein.Groups":1,"Percolator.q.Value":0.00016,"Percolator.PEP":0.0001,"Percolator.SVMScore":1.833,"Precursor.Area":875093.5625,"Apex.RT..min.":11.17,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (G4)","Search.ID":"G","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"SSQIHIENESTEDILK","Annotated.Sequence":"[R].SSQIHIENEsTEDILK.[I]","Modifications":"S10(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0068,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":641.62898,"Contaminant":"False","Aligned.m.z..Da.":641.62892,"MH...Da.":1922.87239,"Theo..MH...Da.":1922.87429,"DeltaM..ppm.":-0.99,"Deltam.z..Da.":-0.00064,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1368544.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":66.85934,"Ion.Inject.Time..ms.":22,"RT..min.":11.2623,"First.Scan":10651,"Last.Scan":10651,"Master.Scan.s.":10641,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_06.raw","File.ID":"F30","Quan.Info":"","Peptides.Matched":1752,"XCorr":2.94,"X..Protein.Groups":1,"Percolator.q.Value":0.00016,"Percolator.PEP":0.00002,"Percolator.SVMScore":2.232,"Precursor.Area":640375.6875,"Apex.RT..min.":11.26,"ptmRS..Binomial.Peptide.Score":263.23,"ptmRS..Isoform.Confidence.Probability":0.006,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(7): 787.42; y1+-H(3) O(4) P(6): 700.39; y2+-H(3) O(4) P(7): 394.21","ptmRS..Best.Site.Probabilities":"T11(Phospho): 99.36","ptmRS..Phospho.Site.Probabilities":"S(1): 0.0; S(2): 0.0; S(10): 0.6; T(11): 99.4"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (G4)","Search.ID":"G","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LQETNPEEVPKFEK","Annotated.Sequence":"[K].LQETNPEEVPKFEK.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.5833,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":563.29009,"Contaminant":"False","Aligned.m.z..Da.":563.29015,"MH...Da.":1687.85572,"Theo..MH...Da.":1687.85374,"DeltaM..ppm.":1.17,"Deltam.z..Da.":0.00066,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":4197680.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":27.59746,"Ion.Inject.Time..ms.":22,"RT..min.":9.279,"First.Scan":7939,"Last.Scan":7939,"Master.Scan.s.":7937,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_06.raw","File.ID":"F30","Quan.Info":"","Peptides.Matched":813,"XCorr":3.48,"X..Protein.Groups":1,"Percolator.q.Value":0.00016,"Percolator.PEP":3.675e-06,"Percolator.SVMScore":2.661,"Precursor.Area":3043904.75,"Apex.RT..min.":9.28,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (G4)","Search.ID":"G","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KDELTLEGIK","Annotated.Sequence":"[K].KDELTLEGIK.[Q]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.5815,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":382.55186,"Contaminant":"False","Aligned.m.z..Da.":382.5518,"MH...Da.":1145.64104,"Theo..MH...Da.":1145.64123,"DeltaM..ppm.":-0.17,"Deltam.z..Da.":-0.00006,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1885929.625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":37.85912,"Ion.Inject.Time..ms.":22,"RT..min.":10.2853,"First.Scan":9313,"Last.Scan":9313,"Master.Scan.s.":9302,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_06.raw","File.ID":"F30","Quan.Info":"","Peptides.Matched":311,"XCorr":2.7,"X..Protein.Groups":1,"Percolator.q.Value":0.00016,"Percolator.PEP":0.00023,"Percolator.SVMScore":1.621,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (G4)","Search.ID":"G","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"APQALMLAPTR","Annotated.Sequence":"[K].APQALmLAPTR.[E]","Modifications":"M6(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7165,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":592.82678,"Contaminant":"False","Aligned.m.z..Da.":592.8267,"MH...Da.":1184.64629,"Theo..MH...Da.":1184.6456,"DeltaM..ppm.":0.58,"Deltam.z..Da.":0.00034,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2947572.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":20.60648,"Ion.Inject.Time..ms.":22,"RT..min.":10.1848,"First.Scan":9175,"Last.Scan":9175,"Master.Scan.s.":9172,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_06.raw","File.ID":"F30","Quan.Info":"","Peptides.Matched":320,"XCorr":3.21,"X..Protein.Groups":1,"Percolator.q.Value":0.00016,"Percolator.PEP":3.494e-06,"Percolator.SVMScore":2.673,"Precursor.Area":4526264.5,"Apex.RT..min.":10.21,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (G4)","Search.ID":"G","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KDELTLEGIK","Annotated.Sequence":"[K].KDELTLEGIK.[Q]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":573.32454,"Contaminant":"False","Aligned.m.z..Da.":573.32446,"MH...Da.":1145.64182,"Theo..MH...Da.":1145.64123,"DeltaM..ppm.":0.51,"Deltam.z..Da.":0.00029,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1876483.875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":17.4444,"Ion.Inject.Time..ms.":22,"RT..min.":10.275,"First.Scan":9299,"Last.Scan":9299,"Master.Scan.s.":9289,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_06.raw","File.ID":"F30","Quan.Info":"","Peptides.Matched":302,"XCorr":3.78,"X..Protein.Groups":1,"Percolator.q.Value":0.00016,"Percolator.PEP":2.227e-07,"Percolator.SVMScore":3.366,"Precursor.Area":4693607,"Apex.RT..min.":10.29,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (G4)","Search.ID":"G","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"AGNFALHPEVVR","Annotated.Sequence":"[R].AGNFALHPEVVR.[E]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q03558","Master.Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","Protein.Accessions":"Q03558","Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.6796,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":437.23849,"Contaminant":"False","Aligned.m.z..Da.":437.23844,"MH...Da.":1309.70092,"Theo..MH...Da.":1309.70114,"DeltaM..ppm.":-0.17,"Deltam.z..Da.":-0.00007,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2587831.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":10.0312,"First.Scan":8966,"Last.Scan":8966,"Master.Scan.s.":8964,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_06.raw","File.ID":"F30","Quan.Info":"","Peptides.Matched":389,"XCorr":3.34,"X..Protein.Groups":1,"Percolator.q.Value":0.00016,"Percolator.PEP":2.482e-06,"Percolator.SVMScore":2.759,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (G4)","Search.ID":"G","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].mSEGItDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); T6(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1244,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1096.47129,"Contaminant":"False","Aligned.m.z..Da.":1096.47143,"MH...Da.":2191.9353,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":3.4,"Deltam.z..Da.":0.00373,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":391034.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":15.4845,"First.Scan":16166,"Last.Scan":16166,"Master.Scan.s.":16165,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_06.raw","File.ID":"F30","Quan.Info":"","Peptides.Matched":2194,"XCorr":4.42,"X..Protein.Groups":1,"Percolator.q.Value":0.00016,"Percolator.PEP":7.878e-09,"Percolator.SVMScore":4.207,"Precursor.Area":1016509.75,"Apex.RT..min.":15.45,"ptmRS..Binomial.Peptide.Score":476.32,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(6): 512.24; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+-H(3) O(4) P(9): 869.39; b1+-H(3) O(4) P(10): 998.43; y1+-H(3) O(4) P(14): 1665.76; b1+(3): 259.09; b1+(4): 316.11; b1+(5): 429.20","ptmRS..Best.Site.Probabilities":"T6(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; T(6): 100.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (G4)","Search.ID":"G","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].mSEGItDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); T6(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.1186,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":731.31325,"Contaminant":"False","Aligned.m.z..Da.":731.31335,"MH...Da.":2191.92521,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":-1.2,"Deltam.z..Da.":-0.00088,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":873292.6875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":15.4556,"First.Scan":16129,"Last.Scan":16129,"Master.Scan.s.":16126,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_06.raw","File.ID":"F30","Quan.Info":"","Peptides.Matched":1893,"XCorr":4.13,"X..Protein.Groups":1,"Percolator.q.Value":0.00016,"Percolator.PEP":1.157e-06,"Percolator.SVMScore":2.951,"Precursor.Area":598879.0625,"Apex.RT..min.":15.45,"ptmRS..Binomial.Peptide.Score":405.44,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(6): 512.24; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+-H(3) O(4) P(9): 869.39; b1+-H(3) O(4) P(10): 998.43; y2+-H(3) O(4) P(14): 833.38; b1+(2): 130.05; b1+(3): 259.09; b1+(4): 316.11; b1+(5): 429.20; b2+(3): 130.05","ptmRS..Best.Site.Probabilities":"T6(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; T(6): 100.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (G4)","Search.ID":"G","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0529,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40174,"Contaminant":"False","Aligned.m.z..Da.":967.40187,"MH...Da.":2900.19068,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":-2.98,"Deltam.z..Da.":-0.00288,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1063917.875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":5.09051,"Ion.Inject.Time..ms.":22,"RT..min.":15.4444,"First.Scan":16114,"Last.Scan":16114,"Master.Scan.s.":16113,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_06.raw","File.ID":"F30","Quan.Info":"","Peptides.Matched":2126,"XCorr":5.29,"X..Protein.Groups":1,"Percolator.q.Value":0.00016,"Percolator.PEP":1.877e-07,"Percolator.SVMScore":3.409,"Precursor.Area":3960020.5,"Apex.RT..min.":15.31,"ptmRS..Binomial.Peptide.Score":408.16,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(14): 1621.73; b1+(12): 1344.56","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (G4)","Search.ID":"G","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FDDMELDENLLR","Annotated.Sequence":"[K].FDDMELDENLLR.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5461,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":755.34798,"Contaminant":"False","Aligned.m.z..Da.":755.34791,"MH...Da.":1509.68869,"Theo..MH...Da.":1509.68898,"DeltaM..ppm.":-0.19,"Deltam.z..Da.":-0.00015,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":764013.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":16.0605,"First.Scan":16881,"Last.Scan":16881,"Master.Scan.s.":16871,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_06.raw","File.ID":"F30","Quan.Info":"","Peptides.Matched":1093,"XCorr":3.04,"X..Protein.Groups":1,"Percolator.q.Value":0.00016,"Percolator.PEP":0.00003,"Percolator.SVMScore":2.176,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (G4)","Search.ID":"G","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].msEGITDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S2(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1702,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1096.46588,"Contaminant":"False","Aligned.m.z..Da.":1096.4658,"MH...Da.":2191.92448,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":-1.53,"Deltam.z..Da.":-0.00168,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":293096.71875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":13.60765,"Ion.Inject.Time..ms.":22,"RT..min.":15.9969,"First.Scan":16802,"Last.Scan":16802,"Master.Scan.s.":16793,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_06.raw","File.ID":"F30","Quan.Info":"","Peptides.Matched":1847,"XCorr":3.82,"X..Protein.Groups":1,"Percolator.q.Value":0.00016,"Percolator.PEP":9.279e-08,"Percolator.SVMScore":3.586,"Precursor.Area":17377768,"Apex.RT..min.":15.71,"ptmRS..Binomial.Peptide.Score":485.99,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(2): 112.04; b1+-H(3) O(4) P(3): 241.08; b1+-H(3) O(4) P(4): 298.10; b1+-H(3) O(4) P(5): 411.19; b1+-H(3) O(4) P(6): 512.24; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+-H(3) O(4) P(9): 869.39; b1+-H(3) O(4) P(10): 998.43; y1+(14): 1683.77","ptmRS..Best.Site.Probabilities":"S2(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 100.0; T(6): 0.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (G4)","Search.ID":"G","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"GVFGYGFEEPSAIQQR","Annotated.Sequence":"[R].GVFGYGFEEPSAIQQR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.5146,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":595.62484,"Contaminant":"False","Aligned.m.z..Da.":595.62494,"MH...Da.":1784.85996,"Theo..MH...Da.":1784.86022,"DeltaM..ppm.":-0.15,"Deltam.z..Da.":-0.00009,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":551734.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":9.13294,"Ion.Inject.Time..ms.":22,"RT..min.":15.0396,"First.Scan":15589,"Last.Scan":15589,"Master.Scan.s.":15580,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_06.raw","File.ID":"F30","Quan.Info":"","Peptides.Matched":1263,"XCorr":2.39,"X..Protein.Groups":1,"Percolator.q.Value":0.00016,"Percolator.PEP":0.00007,"Percolator.SVMScore":1.936,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (G4)","Search.ID":"G","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"GVFGYGFEEPSAIQQR","Annotated.Sequence":"[R].GVFGYGFEEPSAIQQR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6728,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":892.9343,"Contaminant":"False","Aligned.m.z..Da.":892.93445,"MH...Da.":1784.86132,"Theo..MH...Da.":1784.86022,"DeltaM..ppm.":0.61,"Deltam.z..Da.":0.00055,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":536150.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":15.0085,"First.Scan":15548,"Last.Scan":15548,"Master.Scan.s.":15541,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_06.raw","File.ID":"F30","Quan.Info":"","Peptides.Matched":1386,"XCorr":3.79,"X..Protein.Groups":1,"Percolator.q.Value":0.00016,"Percolator.PEP":1.594e-08,"Percolator.SVMScore":4.029,"Precursor.Area":1412118.875,"Apex.RT..min.":15.03,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (G4)","Search.ID":"G","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":4,"DeltaScore":0.0946,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40477,"Contaminant":"False","Aligned.m.z..Da.":967.40495,"MH...Da.":2900.19977,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":0.15,"Deltam.z..Da.":0.00015,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":5439119,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":21.73352,"Ion.Inject.Time..ms.":22,"RT..min.":15.0986,"First.Scan":15665,"Last.Scan":15665,"Master.Scan.s.":15658,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_06.raw","File.ID":"F30","Quan.Info":"","Peptides.Matched":2465,"XCorr":6.45,"X..Protein.Groups":1,"Percolator.q.Value":0.00016,"Percolator.PEP":1.992e-11,"Percolator.SVMScore":5.711,"Precursor.Area":44617056,"Apex.RT..min.":15.02,"ptmRS..Binomial.Peptide.Score":667.89,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(13): 1413.58; b1+-H(3) O(4) P(14): 1528.61; b1+-H(3) O(4) P(15): 1642.65; b1+-H(3) O(4) P(16): 1771.69; b1+-H(3) O(4) P(18): 2028.79; y1+-H(3) O(4) P(15): 1750.78; y1+-H(3) O(4) P(14): 1621.73; y1+-H(3) O(4) P(13): 1458.67; b2+-H(3) O(4) P(16): 886.35; b2+-H(3) O(4) P(18): 1014.90; y2+-H(3) O(4) P(14): 811.37; b1+(12): 1344.56; y1+(13): 1556.65","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (G4)","Search.ID":"G","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FTVSAIYSDLPQQER","Annotated.Sequence":"[K].FTVSAIYSDLPQQER.[D]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7035,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":877.43816,"Contaminant":"False","Aligned.m.z..Da.":877.43832,"MH...Da.":1753.86906,"Theo..MH...Da.":1753.87554,"DeltaM..ppm.":-3.7,"Deltam.z..Da.":-0.00324,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1376456.875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":9.60037,"Ion.Inject.Time..ms.":22,"RT..min.":14.9556,"First.Scan":15479,"Last.Scan":15479,"Master.Scan.s.":15476,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_06.raw","File.ID":"F30","Quan.Info":"","Peptides.Matched":1285,"XCorr":3.98,"X..Protein.Groups":1,"Percolator.q.Value":0.00016,"Percolator.PEP":4.838e-08,"Percolator.SVMScore":3.75,"Precursor.Area":1554701.125,"Apex.RT..min.":14.97,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (G4)","Search.ID":"G","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEySDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); Y12(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.3401,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40654,"Contaminant":"False","Aligned.m.z..Da.":967.40671,"MH...Da.":2900.20508,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":1.98,"Deltam.z..Da.":0.00192,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1139856.375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":37.70227,"Ion.Inject.Time..ms.":22,"RT..min.":14.9476,"First.Scan":15469,"Last.Scan":15469,"Master.Scan.s.":15463,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_06.raw","File.ID":"F30","Quan.Info":"","Peptides.Matched":2545,"XCorr":3.94,"X..Protein.Groups":1,"Percolator.q.Value":0.00016,"Percolator.PEP":5.038e-06,"Percolator.SVMScore":2.581,"Precursor.Area":44617056,"Apex.RT..min.":15.02,"ptmRS..Binomial.Peptide.Score":300.74,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(14): 1621.73; y2+-H(3) O(4) P(14): 811.37","ptmRS..Best.Site.Probabilities":"S13(Phospho): 99.99","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (G4)","Search.ID":"G","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FFISNPDLVDRLEK","Annotated.Sequence":"[R].FFISNPDLVDRLEK.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q03558","Master.Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","Protein.Accessions":"Q03558","Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.728,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":564.97028,"Contaminant":"False","Aligned.m.z..Da.":564.97038,"MH...Da.":1692.89631,"Theo..MH...Da.":1692.89555,"DeltaM..ppm.":0.45,"Deltam.z..Da.":0.00025,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":425074.8125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":50.03262,"Ion.Inject.Time..ms.":22,"RT..min.":15.2313,"First.Scan":15838,"Last.Scan":15838,"Master.Scan.s.":15827,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_06.raw","File.ID":"F30","Quan.Info":"","Peptides.Matched":534,"XCorr":2.39,"X..Protein.Groups":1,"Percolator.q.Value":0.00016,"Percolator.PEP":0.00003,"Percolator.SVMScore":2.117,"Precursor.Area":280861.15625,"Apex.RT..min.":15.22,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (G4)","Search.ID":"G","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":4,"DeltaScore":0.0027,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40602,"Contaminant":"False","Aligned.m.z..Da.":967.4057,"MH...Da.":2900.2035,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":1.44,"Deltam.z..Da.":0.00139,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":335608.6875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":72.76016,"Ion.Inject.Time..ms.":22,"RT..min.":16.6948,"First.Scan":17593,"Last.Scan":17593,"Master.Scan.s.":17586,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_06.raw","File.ID":"F30","Quan.Info":"","Peptides.Matched":2594,"XCorr":3.69,"X..Protein.Groups":1,"Percolator.q.Value":0.00016,"Percolator.PEP":4.912e-06,"Percolator.SVMScore":2.588,"ptmRS..Binomial.Peptide.Score":222.87,"ptmRS..Isoform.Confidence.Probability":0.5,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"Y12(Phospho): 50; S13(Phospho): 50","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 50.0; S(13): 50.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (G4)","Search.ID":"G","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MFILDEADEMLSSGFK","Annotated.Sequence":"[K].mFILDEADEmLSSGFK.[E]","Modifications":"M1(Oxidation); M10(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.7593,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":622.28333,"Contaminant":"False","Aligned.m.z..Da.":622.28312,"MH...Da.":1864.83544,"Theo..MH...Da.":1864.83433,"DeltaM..ppm.":0.59,"Deltam.z..Da.":0.00037,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":678829.4375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":22.17938,"Ion.Inject.Time..ms.":22,"RT..min.":16.6939,"First.Scan":17592,"Last.Scan":17592,"Master.Scan.s.":17586,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_06.raw","File.ID":"F30","Quan.Info":"","Peptides.Matched":1890,"XCorr":2.95,"X..Protein.Groups":1,"Percolator.q.Value":0.00016,"Percolator.PEP":0.00002,"Percolator.SVMScore":2.274,"Precursor.Area":273249.09375,"Apex.RT..min.":16.7,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (G4)","Search.ID":"G","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MFILDEADEMLSSGFK","Annotated.Sequence":"[K].mFILDEADEmLSSGFK.[E]","Modifications":"M1(Oxidation); M10(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6997,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":932.92278,"Contaminant":"False","Aligned.m.z..Da.":932.92248,"MH...Da.":1864.83829,"Theo..MH...Da.":1864.83433,"DeltaM..ppm.":2.13,"Deltam.z..Da.":0.00198,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":484355.4375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":14.5989,"Ion.Inject.Time..ms.":22,"RT..min.":16.6717,"First.Scan":17569,"Last.Scan":17569,"Master.Scan.s.":17560,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_06.raw","File.ID":"F30","Quan.Info":"","Peptides.Matched":1890,"XCorr":3.33,"X..Protein.Groups":1,"Percolator.q.Value":0.00016,"Percolator.PEP":5.97e-08,"Percolator.SVMScore":3.697,"Precursor.Area":1013316.5,"Apex.RT..min.":16.7,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (G4)","Search.ID":"G","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DIFSNDELLSDAYDAK","Annotated.Sequence":"[K].DIFSNDELLSDAYDAK.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.6821,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":605.94827,"Contaminant":"False","Aligned.m.z..Da.":605.94812,"MH...Da.":1815.83026,"Theo..MH...Da.":1815.82831,"DeltaM..ppm.":1.07,"Deltam.z..Da.":0.00065,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":799585.1875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":12.00686,"Ion.Inject.Time..ms.":22,"RT..min.":17.1015,"First.Scan":17997,"Last.Scan":17997,"Master.Scan.s.":17989,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_06.raw","File.ID":"F30","Quan.Info":"","Peptides.Matched":1695,"XCorr":2.8,"X..Protein.Groups":1,"Percolator.q.Value":0.00016,"Percolator.PEP":0.00002,"Percolator.SVMScore":2.228,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (G4)","Search.ID":"G","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DIFSNDELLSDAYDAK","Annotated.Sequence":"[K].DIFSNDELLSDAYDAK.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6709,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":908.41855,"Contaminant":"False","Aligned.m.z..Da.":908.41831,"MH...Da.":1815.82983,"Theo..MH...Da.":1815.82831,"DeltaM..ppm.":0.83,"Deltam.z..Da.":0.00076,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":715455.6875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":17.0869,"First.Scan":17983,"Last.Scan":17983,"Master.Scan.s.":17976,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_06.raw","File.ID":"F30","Quan.Info":"","Peptides.Matched":1573,"XCorr":3.92,"X..Protein.Groups":1,"Percolator.q.Value":0.00016,"Percolator.PEP":1.574e-08,"Percolator.SVMScore":4.032,"Precursor.Area":3795211.75,"Apex.RT..min.":17.11,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (G4)","Search.ID":"G","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DFLLKPELSR","Annotated.Sequence":"[K].DFLLKPELSR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.6767,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":406.56754,"Contaminant":"False","Aligned.m.z..Da.":406.5675,"MH...Da.":1217.68809,"Theo..MH...Da.":1217.68885,"DeltaM..ppm.":-0.62,"Deltam.z..Da.":-0.00025,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1142953,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":13.83095,"Ion.Inject.Time..ms.":22,"RT..min.":13.1576,"First.Scan":13144,"Last.Scan":13144,"Master.Scan.s.":13137,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_06.raw","File.ID":"F30","Quan.Info":"","Peptides.Matched":285,"XCorr":2.66,"X..Protein.Groups":1,"Percolator.q.Value":0.00016,"Percolator.PEP":0.00008,"Percolator.SVMScore":1.888,"Precursor.Area":1156919.625,"Apex.RT..min.":13.17,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (G4)","Search.ID":"G","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"AIMPIIEGHDVLAQAQSGTGK","Annotated.Sequence":"[R].AImPIIEGHDVLAQAQSGTGK.[T]","Modifications":"M3(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.6218,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":718.04076,"Contaminant":"False","Aligned.m.z..Da.":718.04072,"MH...Da.":2152.10772,"Theo..MH...Da.":2152.10668,"DeltaM..ppm.":0.48,"Deltam.z..Da.":0.00035,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":471901.40625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":21.48692,"Ion.Inject.Time..ms.":22,"RT..min.":12.5923,"First.Scan":12415,"Last.Scan":12415,"Master.Scan.s.":12409,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_06.raw","File.ID":"F30","Quan.Info":"","Peptides.Matched":971,"XCorr":3.94,"X..Protein.Groups":1,"Percolator.q.Value":0.00016,"Percolator.PEP":1.535e-08,"Percolator.SVMScore":4.039,"Precursor.Area":1460230,"Apex.RT..min.":12.62,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (G4)","Search.ID":"G","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FDDMELDENLLR","Annotated.Sequence":"[K].FDDmELDENLLR.[G]","Modifications":"M4(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5679,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":763.3456,"Contaminant":"False","Aligned.m.z..Da.":763.34568,"MH...Da.":1525.68393,"Theo..MH...Da.":1525.6839,"DeltaM..ppm.":0.02,"Deltam.z..Da.":0.00002,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":486809.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":14.4759,"First.Scan":14859,"Last.Scan":14859,"Master.Scan.s.":14853,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_06.raw","File.ID":"F30","Quan.Info":"","Peptides.Matched":1099,"XCorr":2.87,"X..Protein.Groups":1,"Percolator.q.Value":0.00016,"Percolator.PEP":0.00002,"Percolator.SVMScore":2.283,"Precursor.Area":1232090.25,"Apex.RT..min.":14.51,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (G4)","Search.ID":"G","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FDDMELDENLLR","Annotated.Sequence":"[K].FDDmELDENLLR.[G]","Modifications":"M4(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":3,"DeltaScore":0.628,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":763.34621,"Contaminant":"False","Aligned.m.z..Da.":763.3463,"MH...Da.":1525.68514,"Theo..MH...Da.":1525.6839,"DeltaM..ppm.":0.81,"Deltam.z..Da.":0.00062,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1309240.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":45.52824,"Ion.Inject.Time..ms.":22,"RT..min.":14.5349,"First.Scan":14934,"Last.Scan":14934,"Master.Scan.s.":14931,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_06.raw","File.ID":"F30","Quan.Info":"","Peptides.Matched":1083,"XCorr":3.36,"X..Protein.Groups":1,"Percolator.q.Value":0.00016,"Percolator.PEP":1.474e-06,"Percolator.SVMScore":2.89,"Precursor.Area":1232090.25,"Apex.RT..min.":14.51,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (G4)","Search.ID":"G","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].mSEGITDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7412,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1056.48467,"Contaminant":"False","Aligned.m.z..Da.":1056.4848,"MH...Da.":2111.96207,"Theo..MH...Da.":2111.96151,"DeltaM..ppm.":0.26,"Deltam.z..Da.":0.00028,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":498873.46875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":26.20163,"Ion.Inject.Time..ms.":22,"RT..min.":14.5878,"First.Scan":15003,"Last.Scan":15003,"Master.Scan.s.":14996,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_06.raw","File.ID":"F30","Quan.Info":"","Peptides.Matched":2334,"XCorr":4.52,"X..Protein.Groups":1,"Percolator.q.Value":0.00016,"Percolator.PEP":2.818e-09,"Percolator.SVMScore":4.465,"Precursor.Area":1051240.5,"Apex.RT..min.":14.61,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (G4)","Search.ID":"G","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FDDMELDENLLR","Annotated.Sequence":"[K].FDDmELDENLLR.[G]","Modifications":"M4(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":4,"DeltaScore":0.4489,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":763.34578,"Contaminant":"False","Aligned.m.z..Da.":763.34587,"MH...Da.":1525.68428,"Theo..MH...Da.":1525.6839,"DeltaM..ppm.":0.25,"Deltam.z..Da.":0.00019,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":419101.28125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":86.76436,"Ion.Inject.Time..ms.":22,"RT..min.":14.567,"First.Scan":14976,"Last.Scan":14976,"Master.Scan.s.":14970,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_06.raw","File.ID":"F30","Quan.Info":"","Peptides.Matched":1088,"XCorr":2.25,"X..Protein.Groups":1,"Percolator.q.Value":0.00016,"Percolator.PEP":0.00003,"Percolator.SVMScore":2.136,"Precursor.Area":1232090.25,"Apex.RT..min.":14.51,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (G4)","Search.ID":"G","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0772,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":953.4001,"Contaminant":"False","Aligned.m.z..Da.":953.40003,"MH...Da.":2858.18574,"Theo..MH...Da.":2858.18876,"DeltaM..ppm.":-1.06,"Deltam.z..Da.":-0.00101,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":686667.1875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":8.63122,"Ion.Inject.Time..ms.":22,"RT..min.":13.7255,"First.Scan":13882,"Last.Scan":13882,"Master.Scan.s.":13878,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_06.raw","File.ID":"F30","Quan.Info":"","Peptides.Matched":2347,"XCorr":5.18,"X..Protein.Groups":1,"Percolator.q.Value":0.00016,"Percolator.PEP":1.425e-06,"Percolator.SVMScore":2.899,"Precursor.Area":1242040.25,"Apex.RT..min.":13.75,"ptmRS..Binomial.Peptide.Score":409.28,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(16): 1729.68; y1+-H(3) O(4) P(13): 1458.67; y2+-H(3) O(4) P(14): 811.37; b1+(12): 1302.55","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (G4)","Search.ID":"G","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KFQDDTLNR","Annotated.Sequence":"[K].KFQDDtLNR.[V]","Modifications":"T6(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7049,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":608.77117,"Contaminant":"False","Aligned.m.z..Da.":608.77111,"MH...Da.":1216.53506,"Theo..MH...Da.":1216.53579,"DeltaM..ppm.":-0.6,"Deltam.z..Da.":-0.00037,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2527931,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":28.58221,"Ion.Inject.Time..ms.":22,"RT..min.":6.2384,"First.Scan":3704,"Last.Scan":3704,"Master.Scan.s.":3699,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_06.raw","File.ID":"F30","Quan.Info":"","Peptides.Matched":608,"XCorr":3.05,"X..Protein.Groups":1,"Percolator.q.Value":0.00016,"Percolator.PEP":0.00007,"Percolator.SVMScore":1.934,"Precursor.Area":3796125.5,"Apex.RT..min.":6.25,"ptmRS..Binomial.Peptide.Score":378.44,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"T6(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"T(6): 100.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (G4)","Search.ID":"G","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"NHQLEEEIEK","Annotated.Sequence":"[K].NHQLEEEIEK.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5125,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":634.80952,"Contaminant":"False","Aligned.m.z..Da.":634.80953,"MH...Da.":1268.61177,"Theo..MH...Da.":1268.61172,"DeltaM..ppm.":0.04,"Deltam.z..Da.":0.00002,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2396791.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":70.84481,"Ion.Inject.Time..ms.":22,"RT..min.":7.1403,"First.Scan":5133,"Last.Scan":5133,"Master.Scan.s.":5129,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_06.raw","File.ID":"F30","Quan.Info":"","Peptides.Matched":880,"XCorr":1.6,"X..Protein.Groups":1,"Percolator.q.Value":0.00016,"Percolator.PEP":0.0005,"Percolator.SVMScore":1.418,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (G4)","Search.ID":"G","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"AAEAGETGAATSATEGDNNNNTAAGDKK","Annotated.Sequence":"[K].AAEAGETGAATSATEGDNNNNTAAGDKK.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.7357,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":879.39395,"Contaminant":"False","Aligned.m.z..Da.":879.39386,"MH...Da.":2636.16731,"Theo..MH...Da.":2636.16665,"DeltaM..ppm.":0.25,"Deltam.z..Da.":0.00022,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":4776593.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":5.0213,"First.Scan":2054,"Last.Scan":2054,"Master.Scan.s.":2048,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_06.raw","File.ID":"F30","Quan.Info":"","Peptides.Matched":3606,"XCorr":8.21,"X..Protein.Groups":1,"Percolator.q.Value":0.00016,"Percolator.PEP":5.047e-18,"Percolator.SVMScore":9.532,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (G4)","Search.ID":"G","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"AAEAGETGAATSATEGDNNNNTAAGDKK","Annotated.Sequence":"[K].AAEAGETGAATsATEGDNNNNTAAGDKK.[G]","Modifications":"S12(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0452,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":906.049,"Contaminant":"False","Aligned.m.z..Da.":906.0489,"MH...Da.":2716.13245,"Theo..MH...Da.":2716.13298,"DeltaM..ppm.":-0.2,"Deltam.z..Da.":-0.00018,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":8102747,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":2.44476,"Ion.Inject.Time..ms.":18.926,"RT..min.":5.0815,"First.Scan":2118,"Last.Scan":2118,"Master.Scan.s.":2113,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_06.raw","File.ID":"F30","Quan.Info":"","Peptides.Matched":2543,"XCorr":5.97,"X..Protein.Groups":1,"Percolator.q.Value":0.00016,"Percolator.PEP":3.235e-13,"Percolator.SVMScore":6.748,"ptmRS..Binomial.Peptide.Score":469.02,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(15): 1300.57; y1+-H(3) O(4) P(18): 1789.79; y1+-H(3) O(4) P(17): 1688.75; y2+-H(3) O(4) P(24): 1138.50; y2+-H(3) O(4) P(18): 895.40; y2+-H(3) O(4) P(17): 844.88","ptmRS..Best.Site.Probabilities":"S12(Phospho): 99.99","ptmRS..Phospho.Site.Probabilities":"T(7): 0.0; T(11): 0.0; S(12): 100.0; T(14): 0.0; T(22): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (G4)","Search.ID":"G","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KSDEPSRESTPVR","Annotated.Sequence":"[K].KSDEPsREsTPVR.[S]","Modifications":"S6(Phospho); S9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q08421","Master.Protein.Descriptions":"Enhancer of translation termination 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ETT1 PE=1 SV=1","Protein.Accessions":"Q08421","Protein.Descriptions":"Enhancer of translation termination 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ETT1 PE=1 SV=1","X..Missed.Cleavages":2,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.053,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":824.34192,"Contaminant":"False","Aligned.m.z..Da.":824.34176,"MH...Da.":1647.67656,"Theo..MH...Da.":1647.67752,"DeltaM..ppm.":-0.58,"Deltam.z..Da.":-0.00048,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":21583788,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":10.76613,"Ion.Inject.Time..ms.":6.289,"RT..min.":4.4296,"First.Scan":1427,"Last.Scan":1427,"Master.Scan.s.":1424,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_06.raw","File.ID":"F30","Quan.Info":"","Peptides.Matched":971,"XCorr":2.83,"X..Protein.Groups":1,"Percolator.q.Value":0.00016,"Percolator.PEP":0.00041,"Percolator.SVMScore":1.469,"Precursor.Area":11240974,"Apex.RT..min.":4.43,"ptmRS..Binomial.Peptide.Score":356.33,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(9): 998.45; b1+-H(3) O(4) P(10): 1179.47; b1+-H(3) O(4) P(12): 1375.59; y1+- -2H(3) O(4) P -2H(3) O(4) P(10): 1121.57; y1+- -2H(3) O(4) P -2H(3) O(4) P(9): 992.53; y1+- -2H(3) O(4) P -2H(3) O(4) P(5): 523.30; y2+- -2H(3) O(4) P -2H(3) O(4) P(10): 561.29; y2+- -2H(3) O(4) P -2H(3) O(4) P(9): 496.77; b1+(8): 929.43","ptmRS..Best.Site.Probabilities":"S9(Phospho): 99.49; T10(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(6): 0.5; S(9): 99.5; T(10): 100.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (G4)","Search.ID":"G","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LQETNPEEVPK","Annotated.Sequence":"[K].LQETNPEEVPK.[F]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5737,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":642.32812,"Contaminant":"False","Aligned.m.z..Da.":642.32817,"MH...Da.":1283.64896,"Theo..MH...Da.":1283.64777,"DeltaM..ppm.":0.93,"Deltam.z..Da.":0.00059,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":4757702,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":14.17918,"Ion.Inject.Time..ms.":22,"RT..min.":7.5947,"First.Scan":5744,"Last.Scan":5744,"Master.Scan.s.":5740,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_06.raw","File.ID":"F30","Quan.Info":"","Peptides.Matched":516,"XCorr":2.51,"X..Protein.Groups":1,"Percolator.q.Value":0.00016,"Percolator.PEP":0.00008,"Percolator.SVMScore":1.897,"Precursor.Area":10982917,"Apex.RT..min.":7.62,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (G4)","Search.ID":"G","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"IGNNELLHR","Annotated.Sequence":"[K].IGNNELLHR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q03558","Master.Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","Protein.Accessions":"Q03558","Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.5381,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":355.86491,"Contaminant":"False","Aligned.m.z..Da.":355.86494,"MH...Da.":1065.58019,"Theo..MH...Da.":1065.57997,"DeltaM..ppm.":0.21,"Deltam.z..Da.":0.00007,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2796253,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":15.00762,"Ion.Inject.Time..ms.":22,"RT..min.":7.5971,"First.Scan":5748,"Last.Scan":5748,"Master.Scan.s.":5740,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_06.raw","File.ID":"F30","Quan.Info":"","Peptides.Matched":300,"XCorr":2.36,"X..Protein.Groups":1,"Percolator.q.Value":0.00016,"Percolator.PEP":0.00043,"Percolator.SVMScore":1.453,"Precursor.Area":1769580,"Apex.RT..min.":7.59,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (G4)","Search.ID":"G","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSSPLHRLSPTGK","Annotated.Sequence":"[R].mSsPLHRLsPTGK.[S]","Modifications":"M1(Oxidation); S3(Phospho); S9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.4152,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":793.84378,"Contaminant":"False","Aligned.m.z..Da.":793.84386,"MH...Da.":1586.68029,"Theo..MH...Da.":1586.67977,"DeltaM..ppm.":0.32,"Deltam.z..Da.":0.00026,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3402534.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":7.6868,"First.Scan":5864,"Last.Scan":5864,"Master.Scan.s.":5857,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_06.raw","File.ID":"F30","Quan.Info":"","Peptides.Matched":982,"XCorr":2.77,"X..Protein.Groups":1,"Percolator.q.Value":0.00016,"Percolator.PEP":0.00036,"Percolator.SVMScore":1.499,"Precursor.Area":4000344,"Apex.RT..min.":7.7,"ptmRS..Binomial.Peptide.Score":380.13,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(8): 877.50; y1+-H(3) O(4) P(5): 471.26; y2+-H(3) O(4) P(11): 627.82; y2+-H(3) O(4) P(10): 544.32; y2+-H(3) O(4) P(8): 439.25; b1+- -2H(3) O(4) P -2H(3) O(4) P(9): 989.50; y1+(4): 402.23; y1+(3): 305.18","ptmRS..Best.Site.Probabilities":"S3(Phospho): 100; S9(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(3): 100.0; S(9): 100.0; T(11): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (G4)","Search.ID":"G","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"DFRHSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[K].DFRHSDsYsENETNHTNVPISSTGGTNNK.[T]","Modifications":"S7(Phospho); S9(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":1,"Charge":4,"Original.Precursor.Charge":4,"DeltaScore":0.0415,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":842.84226,"Contaminant":"False","Aligned.m.z..Da.":842.84241,"MH...Da.":3368.34724,"Theo..MH...Da.":3368.34888,"DeltaM..ppm.":-0.49,"Deltam.z..Da.":-0.00041,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1903378.625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":8.5892,"First.Scan":7021,"Last.Scan":7021,"Master.Scan.s.":7014,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_06.raw","File.ID":"F30","Quan.Info":"","Peptides.Matched":927,"XCorr":6.98,"X..Protein.Groups":1,"Percolator.q.Value":0.00016,"Percolator.PEP":1.686e-08,"Percolator.SVMScore":4.015,"Precursor.Area":2625852.25,"Apex.RT..min.":8.59,"ptmRS..Binomial.Peptide.Score":379.56,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"b2+-H(3) O(4) P(8): 535.69; b2+- -2H(3) O(4) P -2H(3) O(4) P(15): 892.36; b2+- -2H(3) O(4) P -2H(3) O(4) P(16): 942.88; b2+- -2H(3) O(4) P -2H(3) O(4) P(17): 999.90; b2+- -2H(3) O(4) P -2H(3) O(4) P(18): 1049.44","ptmRS..Best.Site.Probabilities":"S5(Phospho): 99.98; S7(Phospho): 98.78","ptmRS..Phospho.Site.Probabilities":"S(5): 100.0; S(7): 98.8; Y(8): 0.0; S(9): 1.2; T(13): 0.0; T(16): 0.0; S(21): 0.0; S(22): 0.0; T(23): 0.0; T(26): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (G4)","Search.ID":"G","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSSPLHRLSPTGK","Annotated.Sequence":"[R].mSsPLHRLsPTGK.[S]","Modifications":"M1(Oxidation); S3(Phospho); S9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.4779,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":529.56492,"Contaminant":"False","Aligned.m.z..Da.":529.56497,"MH...Da.":1586.68023,"Theo..MH...Da.":1586.67977,"DeltaM..ppm.":0.29,"Deltam.z..Da.":0.00015,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2517902.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":43.45491,"Ion.Inject.Time..ms.":22,"RT..min.":7.6691,"First.Scan":5842,"Last.Scan":5842,"Master.Scan.s.":5831,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_06.raw","File.ID":"F30","Quan.Info":"","Peptides.Matched":983,"XCorr":3.39,"X..Protein.Groups":1,"Percolator.q.Value":0.00016,"Percolator.PEP":0.00033,"Percolator.SVMScore":1.525,"ptmRS..Binomial.Peptide.Score":227.92,"ptmRS..Isoform.Confidence.Probability":0.993,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(9): 990.58; y1+-H(3) O(4) P(8): 877.50; y2+-H(3) O(4) P(10): 544.32; y2+-H(3) O(4) P(8): 439.25; y1+(4): 402.23","ptmRS..Best.Site.Probabilities":"S3(Phospho): 99.99; S9(Phospho): 99.28","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(3): 100.0; S(9): 99.3; T(11): 0.7"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (G4)","Search.ID":"G","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"HSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[R].HSDsYsENETNHTNVPISSTGGTNNK.[T]","Modifications":"S4(Phospho); S6(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.1426,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":984.05573,"Contaminant":"False","Aligned.m.z..Da.":984.05585,"MH...Da.":2950.15263,"Theo..MH...Da.":2950.15241,"DeltaM..ppm.":0.08,"Deltam.z..Da.":0.00007,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":4606883,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":7.9372,"First.Scan":6186,"Last.Scan":6186,"Master.Scan.s.":6182,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_06.raw","File.ID":"F30","Quan.Info":"","Peptides.Matched":1123,"XCorr":6.1,"X..Protein.Groups":1,"Percolator.q.Value":0.00016,"Percolator.PEP":5.705e-08,"Percolator.SVMScore":3.708,"Precursor.Area":7396007,"Apex.RT..min.":7.95,"ptmRS..Binomial.Peptide.Score":605.28,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(6): 739.21; y2+-H(3) O(4) P(22): 1173.53; y2+-H(3) O(4) P(21): 1092.00; b1+- -2H(3) O(4) P -2H(3) O(4) P(6): 641.23; b1+- -2H(3) O(4) P -2H(3) O(4) P(11): 1228.45; b2+- -2H(3) O(4) P -2H(3) O(4) P(13): 733.78; b2+- -2H(3) O(4) P -2H(3) O(4) P(14): 790.80; b2+- -2H(3) O(4) P -2H(3) O(4) P(15): 840.34","ptmRS..Best.Site.Probabilities":"S4(Phospho): 100; S6(Phospho): 99.99","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(4): 100.0; Y(5): 0.0; S(6): 100.0; T(10): 0.0; T(13): 0.0; S(18): 0.0; S(19): 0.0; T(20): 0.0; T(23): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (G4)","Search.ID":"G","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"HSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[R].HSDSYsENETNHTNVPISSTGGTNNK.[T]","Modifications":"S6(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0283,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":957.39976,"Contaminant":"False","Aligned.m.z..Da.":957.39982,"MH...Da.":2870.18474,"Theo..MH...Da.":2870.18608,"DeltaM..ppm.":-0.47,"Deltam.z..Da.":-0.00045,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3831392.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":7.485,"First.Scan":5600,"Last.Scan":5600,"Master.Scan.s.":5597,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_06.raw","File.ID":"F30","Quan.Info":"","Peptides.Matched":2478,"XCorr":6.71,"X..Protein.Groups":1,"Percolator.q.Value":0.00016,"Percolator.PEP":1.02e-10,"Percolator.SVMScore":5.3,"Precursor.Area":15525033,"Apex.RT..min.":7.5,"ptmRS..Binomial.Peptide.Score":593.23,"ptmRS..Isoform.Confidence.Probability":0.994,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(6): 659.24; b1+-H(3) O(4) P(9): 1031.37; b1+-H(3) O(4) P(10): 1132.42; b1+-H(3) O(4) P(11): 1246.46; b1+-H(3) O(4) P(12): 1383.52; b1+-H(3) O(4) P(15): 1697.68; b2+-H(3) O(4) P(12): 692.26; b2+-H(3) O(4) P(13): 742.79; b2+-H(3) O(4) P(14): 799.81; b2+-H(3) O(4) P(15): 849.34; y2+-H(3) O(4) P(25): 1318.08; y2+-H(3) O(4) P(24): 1274.56; y2+-H(3) O(4) P(23): 1217.05; y2+-H(3) O(4) P(21): 1092.00; b1+(4): 427.16","ptmRS..Best.Site.Probabilities":"S6(Phospho): 99.43","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(4): 0.6; Y(5): 0.0; S(6): 99.4; T(10): 0.0; T(13): 0.0; S(18): 0.0; S(19): 0.0; T(20): 0.0; T(23): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (G4)","Search.ID":"G","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"IQDPQMTGYVSTR","Annotated.Sequence":"[R].IQDPQmTGyVSTR.[Y]","Modifications":"M6(Oxidation); Y9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P32485","Master.Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","Protein.Accessions":"P32485","Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1203,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":796.34767,"Contaminant":"False","Aligned.m.z..Da.":796.34773,"MH...Da.":1591.68807,"Theo..MH...Da.":1591.6822,"DeltaM..ppm.":3.69,"Deltam.z..Da.":0.00293,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1913397.125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":11.29903,"Ion.Inject.Time..ms.":22,"RT..min.":7.5697,"First.Scan":5713,"Last.Scan":5713,"Master.Scan.s.":5701,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_06.raw","File.ID":"F30","Quan.Info":"","Peptides.Matched":1163,"XCorr":2.91,"X..Protein.Groups":1,"Percolator.q.Value":0.00016,"Percolator.PEP":4.293e-06,"Percolator.SVMScore":2.621,"ptmRS..Binomial.Peptide.Score":286.35,"ptmRS..Isoform.Confidence.Probability":0.333,"ptmRS..Isoform.Group.Report":"y1+(4): 462.27; y1+(3): 363.20","ptmRS..Best.Site.Probabilities":"Y9(Phospho): 33.28; S11(Phospho): 33.28; T12(Phospho): 33.28","ptmRS..Phospho.Site.Probabilities":"T(7): 0.2; Y(9): 33.3; S(11): 33.3; T(12): 33.3"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Y4)","Search.ID":"Y","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].msEGITDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S2(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0679,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":731.31331,"Contaminant":"False","Aligned.m.z..Da.":731.3134,"MH...Da.":2191.9254,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":-1.11,"Deltam.z..Da.":-0.00081,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":289096.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":11.81206,"Ion.Inject.Time..ms.":22,"RT..min.":15.6628,"First.Scan":16125,"Last.Scan":16125,"Master.Scan.s.":16113,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_06.raw","File.ID":"F12","Quan.Info":"","Peptides.Matched":1885,"XCorr":3.83,"X..Protein.Groups":1,"Percolator.q.Value":0.00016,"Percolator.PEP":0.00001,"Percolator.SVMScore":2.054,"Precursor.Area":341172.78125,"Apex.RT..min.":15.67,"ptmRS..Binomial.Peptide.Score":414.81,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(2): 112.04; b1+-H(3) O(4) P(3): 241.08; b1+-H(3) O(4) P(4): 298.10; b1+-H(3) O(4) P(5): 411.19; b1+-H(3) O(4) P(6): 512.24; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+-H(3) O(4) P(9): 869.39; b1+-H(3) O(4) P(10): 998.43","ptmRS..Best.Site.Probabilities":"S2(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 100.0; T(6): 0.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Y4)","Search.ID":"Y","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].msEGITDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S2(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1632,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1096.46751,"Contaminant":"False","Aligned.m.z..Da.":1096.46766,"MH...Da.":2191.92774,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":-0.05,"Deltam.z..Da.":-0.00005,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":424078.375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":15.628,"First.Scan":16077,"Last.Scan":16077,"Master.Scan.s.":16074,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_06.raw","File.ID":"F12","Quan.Info":"","Peptides.Matched":1962,"XCorr":4.35,"X..Protein.Groups":1,"Percolator.q.Value":0.00016,"Percolator.PEP":7.413e-09,"Percolator.SVMScore":3.626,"Precursor.Area":3153747.75,"Apex.RT..min.":15.67,"ptmRS..Binomial.Peptide.Score":476.32,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(2): 112.04; b1+-H(3) O(4) P(3): 241.08; b1+-H(3) O(4) P(4): 298.10; b1+-H(3) O(4) P(5): 411.19; b1+-H(3) O(4) P(6): 512.24; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+-H(3) O(4) P(9): 869.39; b1+-H(3) O(4) P(10): 998.43; b1+(4): 396.08; y1+(14): 1683.77","ptmRS..Best.Site.Probabilities":"S2(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 100.0; T(6): 0.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Y4)","Search.ID":"Y","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KFQDDTLNR","Annotated.Sequence":"[K].KFQDDtLNR.[V]","Modifications":"T6(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6489,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":608.77104,"Contaminant":"False","Aligned.m.z..Da.":608.77097,"MH...Da.":1216.5348,"Theo..MH...Da.":1216.53579,"DeltaM..ppm.":-0.81,"Deltam.z..Da.":-0.00049,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1820163.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":28.38554,"Ion.Inject.Time..ms.":22,"RT..min.":6.234,"First.Scan":3745,"Last.Scan":3745,"Master.Scan.s.":3743,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_06.raw","File.ID":"F12","Quan.Info":"","Peptides.Matched":618,"XCorr":2.25,"X..Protein.Groups":1,"Percolator.q.Value":0.00016,"Percolator.PEP":0.00074,"Percolator.SVMScore":1.178,"Precursor.Area":1123216.625,"Apex.RT..min.":6.23,"ptmRS..Binomial.Peptide.Score":339.81,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"T6(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"T(6): 100.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Y4)","Search.ID":"Y","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DIFSNDELLSDAYDAK","Annotated.Sequence":"[K].DIFSNDELLSDAYDAK.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7042,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":908.41705,"Contaminant":"False","Aligned.m.z..Da.":908.4168,"MH...Da.":1815.82683,"Theo..MH...Da.":1815.82831,"DeltaM..ppm.":-0.82,"Deltam.z..Da.":-0.00074,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":361571.375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":8.90974,"Ion.Inject.Time..ms.":22,"RT..min.":17.0949,"First.Scan":17427,"Last.Scan":17427,"Master.Scan.s.":17423,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_06.raw","File.ID":"F12","Quan.Info":"","Peptides.Matched":1650,"XCorr":3.55,"X..Protein.Groups":1,"Percolator.q.Value":0.00016,"Percolator.PEP":1.314e-10,"Percolator.SVMScore":4.483,"Precursor.Area":1070020.125,"Apex.RT..min.":17.11,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Y4)","Search.ID":"Y","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FDDMELDENLLR","Annotated.Sequence":"[K].FDDmELDENLLR.[G]","Modifications":"M4(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5543,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":763.34604,"Contaminant":"False","Aligned.m.z..Da.":763.34625,"MH...Da.":1525.68481,"Theo..MH...Da.":1525.6839,"DeltaM..ppm.":0.6,"Deltam.z..Da.":0.00046,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":201350.3125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":40.13903,"Ion.Inject.Time..ms.":22,"RT..min.":14.4997,"First.Scan":14771,"Last.Scan":14771,"Master.Scan.s.":14765,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_06.raw","File.ID":"F12","Quan.Info":"","Peptides.Matched":1089,"XCorr":2.67,"X..Protein.Groups":1,"Percolator.q.Value":0.00016,"Percolator.PEP":9.978e-07,"Percolator.SVMScore":2.585,"Precursor.Area":135469.5,"Apex.RT..min.":14.56,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Y4)","Search.ID":"Y","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].mSEGITDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6615,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1056.48523,"Contaminant":"False","Aligned.m.z..Da.":1056.48554,"MH...Da.":2111.96318,"Theo..MH...Da.":2111.96151,"DeltaM..ppm.":0.79,"Deltam.z..Da.":0.00083,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":365094.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":91.82273,"Ion.Inject.Time..ms.":22,"RT..min.":14.6083,"First.Scan":14872,"Last.Scan":14872,"Master.Scan.s.":14868,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_06.raw","File.ID":"F12","Quan.Info":"","Peptides.Matched":2375,"XCorr":3.25,"X..Protein.Groups":1,"Percolator.q.Value":0.00016,"Percolator.PEP":1.726e-09,"Percolator.SVMScore":3.936,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Y4)","Search.ID":"Y","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FDDMELDENLLR","Annotated.Sequence":"[K].FDDmELDENLLR.[G]","Modifications":"M4(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":3,"DeltaScore":0.5093,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":763.3456,"Contaminant":"False","Aligned.m.z..Da.":763.34583,"MH...Da.":1525.68393,"Theo..MH...Da.":1525.6839,"DeltaM..ppm.":0.02,"Deltam.z..Da.":1e-05,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":127613.14062,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":78.70084,"Ion.Inject.Time..ms.":22,"RT..min.":14.5771,"First.Scan":14843,"Last.Scan":14843,"Master.Scan.s.":14834,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_06.raw","File.ID":"F12","Quan.Info":"","Peptides.Matched":1101,"XCorr":1.61,"X..Protein.Groups":1,"Percolator.q.Value":0.00016,"Percolator.PEP":0.00002,"Percolator.SVMScore":1.938,"Precursor.Area":135469.5,"Apex.RT..min.":14.56,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Y4)","Search.ID":"Y","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":2,"DeltaScore":0.3655,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40555,"Contaminant":"False","Aligned.m.z..Da.":967.40588,"MH...Da.":2900.20208,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":0.95,"Deltam.z..Da.":0.00092,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":733258.875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":31.63584,"Ion.Inject.Time..ms.":22,"RT..min.":15.134,"First.Scan":15488,"Last.Scan":15488,"Master.Scan.s.":15484,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_06.raw","File.ID":"F12","Quan.Info":"","Peptides.Matched":2539,"XCorr":4.98,"X..Protein.Groups":1,"Percolator.q.Value":0.00016,"Percolator.PEP":9.201e-08,"Percolator.SVMScore":3.091,"Precursor.Area":6389594,"Apex.RT..min.":15.02,"ptmRS..Binomial.Peptide.Score":328.42,"ptmRS..Isoform.Confidence.Probability":0.5,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"Y12(Phospho): 50; S13(Phospho): 50","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 50.0; S(13): 50.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Y4)","Search.ID":"Y","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":4,"DeltaScore":0.0605,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40488,"Contaminant":"False","Aligned.m.z..Da.":967.40522,"MH...Da.":2900.20009,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":0.26,"Deltam.z..Da.":0.00025,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1426999.375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":31.5188,"Ion.Inject.Time..ms.":22,"RT..min.":15.1039,"First.Scan":15448,"Last.Scan":15448,"Master.Scan.s.":15445,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_06.raw","File.ID":"F12","Quan.Info":"","Peptides.Matched":2493,"XCorr":4.79,"X..Protein.Groups":1,"Percolator.q.Value":0.00016,"Percolator.PEP":2.537e-11,"Percolator.SVMScore":4.832,"Precursor.Area":6389594,"Apex.RT..min.":15.02,"ptmRS..Binomial.Peptide.Score":552.78,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(13): 1413.58; y1+-H(3) O(4) P(15): 1750.78; y1+-H(3) O(4) P(13): 1458.67; b2+-H(3) O(4) P(16): 886.35; b2+-H(3) O(4) P(18): 1014.90; y2+-H(3) O(4) P(14): 811.37; b1+(12): 1344.56; y1+(13): 1556.65","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Y4)","Search.ID":"Y","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MGSINNNFNTNNNSNTDLDRDWK","Annotated.Sequence":"[-].mGSINNNFNTNNNsNTDLDRDWK.[T]","Modifications":"N-Term(Prot)(Met-loss); S14(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P39517","Master.Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","Protein.Accessions":"P39517","Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0222,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":878.36935,"Contaminant":"False","Aligned.m.z..Da.":878.36933,"MH...Da.":2633.09351,"Theo..MH...Da.":2633.10123,"DeltaM..ppm.":-2.93,"Deltam.z..Da.":-0.00257,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":650271.8125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":11.2661,"First.Scan":10671,"Last.Scan":10671,"Master.Scan.s.":10659,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_06.raw","File.ID":"F12","Quan.Info":"","Peptides.Matched":2046,"XCorr":3.61,"X..Protein.Groups":1,"Percolator.q.Value":0.00016,"Percolator.PEP":4.694e-06,"Percolator.SVMScore":2.256,"ptmRS..Binomial.Peptide.Score":383,"ptmRS..Isoform.Confidence.Probability":0.992,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(10): 1231.57; y2+-H(3) O(4) P(17): 1025.45; y2+-H(3) O(4) P(16): 968.43; y2+-H(3) O(4) P(15): 894.90; y2+-H(3) O(4) P(14): 837.88; y2+-H(3) O(4) P(13): 787.35; y2+-H(3) O(4) P(12): 730.33; y2+-H(3) O(4) P(11): 673.31; y2+-H(3) O(4) P(10): 616.29; y1+(9): 1162.55","ptmRS..Best.Site.Probabilities":"S14(Phospho): 99.19","ptmRS..Phospho.Site.Probabilities":"S(3): 0.0; T(10): 0.0; S(14): 99.2; T(16): 0.8"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Y4)","Search.ID":"Y","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0514,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40797,"Contaminant":"False","Aligned.m.z..Da.":967.40821,"MH...Da.":2900.20937,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":3.46,"Deltam.z..Da.":0.00335,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":331961.90625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":27.3141,"Ion.Inject.Time..ms.":22,"RT..min.":15.4374,"First.Scan":15850,"Last.Scan":15850,"Master.Scan.s.":15849,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_06.raw","File.ID":"F12","Quan.Info":"","Peptides.Matched":2634,"XCorr":4.86,"X..Protein.Groups":1,"Percolator.q.Value":0.00016,"Percolator.PEP":5.444e-08,"Percolator.SVMScore":3.203,"ptmRS..Binomial.Peptide.Score":366.38,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(14): 1621.73; b1+(12): 1344.56","ptmRS..Best.Site.Probabilities":"S13(Phospho): 99.99","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Y4)","Search.ID":"Y","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":4,"DeltaScore":0.0422,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40557,"Contaminant":"False","Aligned.m.z..Da.":967.40586,"MH...Da.":2900.20214,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":0.97,"Deltam.z..Da.":0.00094,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":303916.0625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":75.78915,"Ion.Inject.Time..ms.":22,"RT..min.":15.2965,"First.Scan":15685,"Last.Scan":15685,"Master.Scan.s.":15677,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_06.raw","File.ID":"F12","Quan.Info":"","Peptides.Matched":2550,"XCorr":4.98,"X..Protein.Groups":1,"Percolator.q.Value":0.00016,"Percolator.PEP":4.915e-08,"Percolator.SVMScore":3.225,"Precursor.Area":6389594,"Apex.RT..min.":15.02,"ptmRS..Binomial.Peptide.Score":297.58,"ptmRS..Isoform.Confidence.Probability":0.991,"ptmRS..Isoform.Group.Report":"b1+(12): 1344.56","ptmRS..Best.Site.Probabilities":"S13(Phospho): 99.13","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.9; S(13): 99.1; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Y4)","Search.ID":"Y","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":5,"DeltaScore":0.0725,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40894,"Contaminant":"False","Aligned.m.z..Da.":967.40929,"MH...Da.":2900.21227,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":4.46,"Deltam.z..Da.":0.00432,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1033729.8125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":25.81791,"Ion.Inject.Time..ms.":22,"RT..min.":14.9764,"First.Scan":15292,"Last.Scan":15292,"Master.Scan.s.":15287,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_06.raw","File.ID":"F12","Quan.Info":"","Peptides.Matched":2681,"XCorr":5.38,"X..Protein.Groups":1,"Percolator.q.Value":0.00016,"Percolator.PEP":8.629e-10,"Percolator.SVMScore":4.083,"ptmRS..Binomial.Peptide.Score":529.02,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(13): 1413.58; b1+-H(3) O(4) P(16): 1771.69; y1+-H(3) O(4) P(14): 1621.73; y1+-H(3) O(4) P(13): 1458.67; b2+-H(3) O(4) P(16): 886.35; b2+-H(3) O(4) P(18): 1014.90; y2+-H(3) O(4) P(14): 811.37; b1+(12): 1344.56; y1+(13): 1556.65","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Y4)","Search.ID":"Y","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEySDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); Y12(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.4282,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40132,"Contaminant":"False","Aligned.m.z..Da.":967.40167,"MH...Da.":2900.18941,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":-3.42,"Deltam.z..Da.":-0.0033,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":447689,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":14.9596,"First.Scan":15270,"Last.Scan":15270,"Master.Scan.s.":15261,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_06.raw","File.ID":"F12","Quan.Info":"","Peptides.Matched":2145,"XCorr":4.18,"X..Protein.Groups":1,"Percolator.q.Value":0.00016,"Percolator.PEP":5.791e-08,"Percolator.SVMScore":3.19,"ptmRS..Binomial.Peptide.Score":291.57,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(14): 1621.73; b2+-H(3) O(4) P(18): 1014.90; y2+-H(3) O(4) P(14): 811.37; b1+(12): 1344.56","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Y4)","Search.ID":"Y","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FTVSAIYSDLPQQER","Annotated.Sequence":"[K].FTVSAIYSDLPQQER.[D]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.674,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":877.44372,"Contaminant":"False","Aligned.m.z..Da.":877.44404,"MH...Da.":1753.88017,"Theo..MH...Da.":1753.87554,"DeltaM..ppm.":2.64,"Deltam.z..Da.":0.00231,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":177923.92188,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":25.07471,"Ion.Inject.Time..ms.":22,"RT..min.":14.9844,"First.Scan":15302,"Last.Scan":15302,"Master.Scan.s.":15300,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_06.raw","File.ID":"F12","Quan.Info":"","Peptides.Matched":1164,"XCorr":3.62,"X..Protein.Groups":1,"Percolator.q.Value":0.00016,"Percolator.PEP":3.685e-08,"Percolator.SVMScore":3.286,"Precursor.Area":171167.1875,"Apex.RT..min.":14.99,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Y4)","Search.ID":"Y","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"GVFGYGFEEPSAIQQR","Annotated.Sequence":"[R].GVFGYGFEEPSAIQQR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.704,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":892.93507,"Contaminant":"False","Aligned.m.z..Da.":892.93539,"MH...Da.":1784.86287,"Theo..MH...Da.":1784.86022,"DeltaM..ppm.":1.48,"Deltam.z..Da.":0.00132,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":286494.375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":15.64322,"Ion.Inject.Time..ms.":22,"RT..min.":15.0415,"First.Scan":15366,"Last.Scan":15366,"Master.Scan.s.":15363,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_06.raw","File.ID":"F12","Quan.Info":"","Peptides.Matched":1422,"XCorr":3.21,"X..Protein.Groups":1,"Percolator.q.Value":0.00016,"Percolator.PEP":3.481e-08,"Percolator.SVMScore":3.298,"Precursor.Area":308007.0625,"Apex.RT..min.":15.05,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Y4)","Search.ID":"Y","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSSPLHRLSPTGK","Annotated.Sequence":"[R].mSsPLHRLsPTGK.[S]","Modifications":"M1(Oxidation); S3(Phospho); S9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.392,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":529.56485,"Contaminant":"False","Aligned.m.z..Da.":529.56483,"MH...Da.":1586.67999,"Theo..MH...Da.":1586.67977,"DeltaM..ppm.":0.14,"Deltam.z..Da.":0.00007,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2140523.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":55.9787,"Ion.Inject.Time..ms.":22,"RT..min.":7.7009,"First.Scan":5850,"Last.Scan":5850,"Master.Scan.s.":5849,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_06.raw","File.ID":"F12","Quan.Info":"","Peptides.Matched":975,"XCorr":3.24,"X..Protein.Groups":1,"Percolator.q.Value":0.00016,"Percolator.PEP":0.0001,"Percolator.SVMScore":1.599,"ptmRS..Binomial.Peptide.Score":215.41,"ptmRS..Isoform.Confidence.Probability":0.991,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(8): 877.50; y2+-H(3) O(4) P(11): 627.82; y2+-H(3) O(4) P(10): 544.32; y2+-H(3) O(4) P(8): 439.25; y1+(4): 402.23","ptmRS..Best.Site.Probabilities":"S3(Phospho): 100; S9(Phospho): 99.1","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(3): 100.0; S(9): 99.1; T(11): 0.9"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Y4)","Search.ID":"Y","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"HSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[R].HSDsYsENETNHTNVPISSTGGTNNK.[T]","Modifications":"S4(Phospho); S6(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.1271,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":984.05657,"Contaminant":"False","Aligned.m.z..Da.":984.05652,"MH...Da.":2950.15517,"Theo..MH...Da.":2950.15241,"DeltaM..ppm.":0.93,"Deltam.z..Da.":0.00092,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2050030.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":7.958,"First.Scan":6195,"Last.Scan":6195,"Master.Scan.s.":6187,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_06.raw","File.ID":"F12","Quan.Info":"","Peptides.Matched":1119,"XCorr":5.82,"X..Protein.Groups":1,"Percolator.q.Value":0.00016,"Percolator.PEP":2.071e-09,"Percolator.SVMScore":3.897,"Precursor.Area":1872527.875,"Apex.RT..min.":7.95,"ptmRS..Binomial.Peptide.Score":569.05,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(4): 409.15; b1+-H(3) O(4) P(5): 572.21; b1+-H(3) O(4) P(6): 739.21; b1+-H(3) O(4) P(9): 1111.34; b1+-H(3) O(4) P(11): 1326.43; b1+-H(3) O(4) P(15): 1777.64; b2+-H(3) O(4) P(12): 732.25; b2+-H(3) O(4) P(14): 839.79; b2+-H(3) O(4) P(15): 889.33; y2+-H(3) O(4) P(23): 1257.03; b1+(2): 225.10; b1+(3): 340.13","ptmRS..Best.Site.Probabilities":"S4(Phospho): 99.99; S6(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(4): 100.0; Y(5): 0.0; S(6): 100.0; T(10): 0.0; T(13): 0.0; S(18): 0.0; S(19): 0.0; T(20): 0.0; T(23): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Y4)","Search.ID":"Y","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"HSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[R].HSDSYsENETNHTNVPISSTGGTNNK.[T]","Modifications":"S6(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0307,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":957.39805,"Contaminant":"False","Aligned.m.z..Da.":957.39802,"MH...Da.":2870.17961,"Theo..MH...Da.":2870.18608,"DeltaM..ppm.":-2.25,"Deltam.z..Da.":-0.00216,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":4034514.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":7.5166,"First.Scan":5605,"Last.Scan":5605,"Master.Scan.s.":5602,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_06.raw","File.ID":"F12","Quan.Info":"","Peptides.Matched":2277,"XCorr":6.19,"X..Protein.Groups":1,"Percolator.q.Value":0.00016,"Percolator.PEP":9.593e-13,"Percolator.SVMScore":5.528,"Precursor.Area":5427558.5,"Apex.RT..min.":7.53,"ptmRS..Binomial.Peptide.Score":554.26,"ptmRS..Isoform.Confidence.Probability":0.987,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(9): 1031.37; b1+-H(3) O(4) P(11): 1246.46; b1+-H(3) O(4) P(12): 1383.52; b1+-H(3) O(4) P(15): 1697.68; b2+-H(3) O(4) P(12): 692.26; b2+-H(3) O(4) P(13): 742.79; b2+-H(3) O(4) P(14): 799.81; b2+-H(3) O(4) P(15): 849.34; y2+-H(3) O(4) P(25): 1318.08; y2+-H(3) O(4) P(23): 1217.05; y2+-H(3) O(4) P(21): 1092.00; b1+(4): 427.16","ptmRS..Best.Site.Probabilities":"S6(Phospho): 98.69","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(4): 0.7; Y(5): 0.0; S(6): 98.7; T(10): 0.7; T(13): 0.0; S(18): 0.0; S(19): 0.0; T(20): 0.0; T(23): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Y4)","Search.ID":"Y","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LQETNPEEVPK","Annotated.Sequence":"[K].LQETNPEEVPK.[F]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.56,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":642.32738,"Contaminant":"False","Aligned.m.z..Da.":642.32736,"MH...Da.":1283.64748,"Theo..MH...Da.":1283.64777,"DeltaM..ppm.":-0.23,"Deltam.z..Da.":-0.00014,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2020677.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":30.9682,"Ion.Inject.Time..ms.":22,"RT..min.":7.6296,"First.Scan":5757,"Last.Scan":5757,"Master.Scan.s.":5745,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_06.raw","File.ID":"F12","Quan.Info":"","Peptides.Matched":569,"XCorr":2.5,"X..Protein.Groups":1,"Percolator.q.Value":0.00016,"Percolator.PEP":0.00012,"Percolator.SVMScore":1.565,"Precursor.Area":2936433.5,"Apex.RT..min.":7.64,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Y4)","Search.ID":"Y","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LQETNPEEVPK","Annotated.Sequence":"[K].LQETNPEEVPK.[F]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":3,"DeltaScore":0.4854,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":642.32785,"Contaminant":"False","Aligned.m.z..Da.":642.32783,"MH...Da.":1283.64843,"Theo..MH...Da.":1283.64777,"DeltaM..ppm.":0.51,"Deltam.z..Da.":0.00033,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3705886.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":58.87617,"Ion.Inject.Time..ms.":19.231,"RT..min.":7.6551,"First.Scan":5790,"Last.Scan":5790,"Master.Scan.s.":5784,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_06.raw","File.ID":"F12","Quan.Info":"","Peptides.Matched":580,"XCorr":1.71,"X..Protein.Groups":1,"Percolator.q.Value":0.00016,"Percolator.PEP":0.00008,"Percolator.SVMScore":1.647,"Precursor.Area":2936433.5,"Apex.RT..min.":7.64,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Y4)","Search.ID":"Y","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"IQDPQMTGYVSTR","Annotated.Sequence":"[R].IQDPQmTGyVSTR.[Y]","Modifications":"M6(Oxidation); Y9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P32485","Master.Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","Protein.Accessions":"P32485","Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1404,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":796.34628,"Contaminant":"False","Aligned.m.z..Da.":796.34626,"MH...Da.":1591.68529,"Theo..MH...Da.":1591.6822,"DeltaM..ppm.":1.94,"Deltam.z..Da.":0.00155,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1928612.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":7.6101,"First.Scan":5731,"Last.Scan":5731,"Master.Scan.s.":5719,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_06.raw","File.ID":"F12","Quan.Info":"","Peptides.Matched":1161,"XCorr":2.85,"X..Protein.Groups":1,"Percolator.q.Value":0.00016,"Percolator.PEP":4.746e-06,"Percolator.SVMScore":2.254,"Precursor.Area":2733784.25,"Apex.RT..min.":7.62,"ptmRS..Binomial.Peptide.Score":270.5,"ptmRS..Isoform.Confidence.Probability":0.986,"ptmRS..Isoform.Group.Report":"y1+(6): 762.32; y1+(5): 705.30","ptmRS..Best.Site.Probabilities":"Y9(Phospho): 98.64","ptmRS..Phospho.Site.Probabilities":"T(7): 0.5; Y(9): 98.6; S(11): 0.5; T(12): 0.5"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Y4)","Search.ID":"Y","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"APQALMLAPTR","Annotated.Sequence":"[K].APQALmLAPTR.[E]","Modifications":"M6(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6773,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":592.82617,"Contaminant":"False","Aligned.m.z..Da.":592.82615,"MH...Da.":1184.64507,"Theo..MH...Da.":1184.6456,"DeltaM..ppm.":-0.45,"Deltam.z..Da.":-0.00027,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1154311.625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":15.71544,"Ion.Inject.Time..ms.":22,"RT..min.":10.2211,"First.Scan":9262,"Last.Scan":9262,"Master.Scan.s.":9255,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_06.raw","File.ID":"F12","Quan.Info":"","Peptides.Matched":325,"XCorr":2.82,"X..Protein.Groups":1,"Percolator.q.Value":0.00016,"Percolator.PEP":0.00003,"Percolator.SVMScore":1.842,"Precursor.Area":1163078.375,"Apex.RT..min.":10.23,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Y4)","Search.ID":"Y","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KDELTLEGIK","Annotated.Sequence":"[K].KDELTLEGIK.[Q]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5069,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":573.32442,"Contaminant":"False","Aligned.m.z..Da.":573.3244,"MH...Da.":1145.64157,"Theo..MH...Da.":1145.64123,"DeltaM..ppm.":0.3,"Deltam.z..Da.":0.00017,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1542890.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":3.15707,"Ion.Inject.Time..ms.":22,"RT..min.":10.2964,"First.Scan":9364,"Last.Scan":9364,"Master.Scan.s.":9359,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_06.raw","File.ID":"F12","Quan.Info":"","Peptides.Matched":307,"XCorr":3.63,"X..Protein.Groups":1,"Percolator.q.Value":0.00016,"Percolator.PEP":6.536e-08,"Percolator.SVMScore":3.164,"Precursor.Area":920182.1875,"Apex.RT..min.":10.3,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Y4)","Search.ID":"Y","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MGSINNNFNTNNNSNTDLDRDWK","Annotated.Sequence":"[-].mGSINNNFNTNNNsNTDLDRDWK.[T]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S14(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P39517","Master.Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","Protein.Accessions":"P39517","Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0663,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":892.37537,"Contaminant":"False","Aligned.m.z..Da.":892.37524,"MH...Da.":2675.11157,"Theo..MH...Da.":2675.11179,"DeltaM..ppm.":-0.08,"Deltam.z..Da.":-0.00007,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":169327.125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":13.4683,"First.Scan":13584,"Last.Scan":13584,"Master.Scan.s.":13580,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_06.raw","File.ID":"F12","Quan.Info":"","Peptides.Matched":2153,"XCorr":3.77,"X..Protein.Groups":1,"Percolator.q.Value":0.00016,"Percolator.PEP":3.74e-07,"Percolator.SVMScore":2.793,"Precursor.Area":537472.0625,"Apex.RT..min.":13.49,"ptmRS..Binomial.Peptide.Score":390.58,"ptmRS..Isoform.Confidence.Probability":0.5,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(10): 1231.57; y2+-H(3) O(4) P(17): 1025.45; y2+-H(3) O(4) P(16): 968.43; y2+-H(3) O(4) P(15): 894.90; y2+-H(3) O(4) P(14): 837.88; y2+-H(3) O(4) P(12): 730.33; y2+-H(3) O(4) P(11): 673.31; y2+-H(3) O(4) P(10): 616.29; y1+(9): 1162.55","ptmRS..Best.Site.Probabilities":"S14(Phospho): 50; T16(Phospho): 50","ptmRS..Phospho.Site.Probabilities":"S(3): 0.0; T(10): 0.0; S(14): 50.0; T(16): 50.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Y4)","Search.ID":"Y","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"IQDPQMTGYVSTR","Annotated.Sequence":"[R].IQDPQMTGyVSTR.[Y]","Modifications":"Y9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P32485","Master.Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","Protein.Accessions":"P32485","Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.08,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":788.3465,"Contaminant":"False","Aligned.m.z..Da.":788.3465,"MH...Da.":1575.68573,"Theo..MH...Da.":1575.68728,"DeltaM..ppm.":-0.99,"Deltam.z..Da.":-0.00078,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1155707.375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":22.05589,"Ion.Inject.Time..ms.":22,"RT..min.":9.5468,"First.Scan":8340,"Last.Scan":8340,"Master.Scan.s.":8332,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_06.raw","File.ID":"F12","Quan.Info":"","Peptides.Matched":1160,"XCorr":2.5,"X..Protein.Groups":1,"Percolator.q.Value":0.00016,"Percolator.PEP":6.331e-06,"Percolator.SVMScore":2.193,"Precursor.Area":941603.25,"Apex.RT..min.":9.55,"ptmRS..Binomial.Peptide.Score":263.02,"ptmRS..Isoform.Confidence.Probability":0.995,"ptmRS..Isoform.Group.Report":"y1+(6): 762.32; y1+(5): 705.30","ptmRS..Best.Site.Probabilities":"Y9(Phospho): 99.48","ptmRS..Phospho.Site.Probabilities":"T(7): 0.5; Y(9): 99.5; S(11): 0.0; T(12): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Y4)","Search.ID":"Y","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"TGTFSIAALQR","Annotated.Sequence":"[K].TGTFSIAALQR.[I]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":3,"DeltaScore":0.573,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":582.82175,"Contaminant":"False","Aligned.m.z..Da.":582.82166,"MH...Da.":1164.63623,"Theo..MH...Da.":1164.63715,"DeltaM..ppm.":-0.79,"Deltam.z..Da.":-0.00046,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":251284.67188,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":71.79245,"Ion.Inject.Time..ms.":22,"RT..min.":13.4262,"First.Scan":13533,"Last.Scan":13533,"Master.Scan.s.":13528,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_06.raw","File.ID":"F12","Quan.Info":"NoneMonoisotopic","Peptides.Matched":384,"XCorr":1.78,"X..Protein.Groups":1,"Percolator.q.Value":0.00016,"Percolator.PEP":0.00106,"Percolator.SVMScore":1.102,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Y4)","Search.ID":"Y","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"AIMPIIEGHDVLAQAQSGTGK","Annotated.Sequence":"[R].AImPIIEGHDVLAQAQSGTGK.[T]","Modifications":"M3(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.6244,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":718.03699,"Contaminant":"False","Aligned.m.z..Da.":718.03688,"MH...Da.":2152.09643,"Theo..MH...Da.":2152.10668,"DeltaM..ppm.":-4.76,"Deltam.z..Da.":-0.00341,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":317917.96875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":15.06833,"Ion.Inject.Time..ms.":22,"RT..min.":12.6849,"First.Scan":12578,"Last.Scan":12578,"Master.Scan.s.":12567,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_06.raw","File.ID":"F12","Quan.Info":"","Peptides.Matched":1152,"XCorr":3.94,"X..Protein.Groups":1,"Percolator.q.Value":0.00016,"Percolator.PEP":4.718e-08,"Percolator.SVMScore":3.233,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Y4)","Search.ID":"Y","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"AAEAGETGAATSATEGDNNNNTAAGDKK","Annotated.Sequence":"[K].AAEAGETGAATsATEGDNNNNTAAGDKK.[G]","Modifications":"S12(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0356,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":906.0503,"Contaminant":"False","Aligned.m.z..Da.":906.05027,"MH...Da.":2716.13635,"Theo..MH...Da.":2716.13298,"DeltaM..ppm.":1.24,"Deltam.z..Da.":0.00112,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2137097,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":4.41099,"Ion.Inject.Time..ms.":22,"RT..min.":5.0588,"First.Scan":2077,"Last.Scan":2077,"Master.Scan.s.":2066,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_06.raw","File.ID":"F12","Quan.Info":"","Peptides.Matched":2509,"XCorr":4.77,"X..Protein.Groups":1,"Percolator.q.Value":0.00016,"Percolator.PEP":2.132e-15,"Percolator.SVMScore":6.998,"ptmRS..Binomial.Peptide.Score":433.4,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(17): 1688.75; y2+-H(3) O(4) P(21): 994.95; y2+-H(3) O(4) P(18): 895.40; y2+-H(3) O(4) P(17): 844.88","ptmRS..Best.Site.Probabilities":"S12(Phospho): 99.99","ptmRS..Phospho.Site.Probabilities":"T(7): 0.0; T(11): 0.0; S(12): 100.0; T(14): 0.0; T(22): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AG4)","Search.ID":"AG","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"IQDPQMTGYVSTR","Annotated.Sequence":"[R].IQDPQMTGyVSTR.[Y]","Modifications":"Y9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P32485","Master.Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","Protein.Accessions":"P32485","Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1034,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":788.34565,"Contaminant":"False","Aligned.m.z..Da.":788.34576,"MH...Da.":1575.68403,"Theo..MH...Da.":1575.68728,"DeltaM..ppm.":-2.06,"Deltam.z..Da.":-0.00163,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1150127.625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":30.24155,"Ion.Inject.Time..ms.":22,"RT..min.":9.593,"First.Scan":7864,"Last.Scan":7864,"Master.Scan.s.":7862,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y25_04.raw","File.ID":"F4","Quan.Info":"","Peptides.Matched":1117,"XCorr":2.61,"X..Protein.Groups":1,"Percolator.q.Value":0.00016,"Percolator.PEP":0.00002,"Percolator.SVMScore":1.668,"ptmRS..Binomial.Peptide.Score":261.42,"ptmRS..Isoform.Confidence.Probability":0.989,"ptmRS..Isoform.Group.Report":"y1+(6): 762.32; y1+(5): 705.30","ptmRS..Best.Site.Probabilities":"Y9(Phospho): 98.94","ptmRS..Phospho.Site.Probabilities":"T(7): 0.5; Y(9): 98.9; S(11): 0.5; T(12): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AG4)","Search.ID":"AG","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEySDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); Y12(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.398,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40239,"Contaminant":"False","Aligned.m.z..Da.":967.40253,"MH...Da.":2900.19262,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":-2.31,"Deltam.z..Da.":-0.00224,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":373987.53125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":15.0533,"First.Scan":14676,"Last.Scan":14676,"Master.Scan.s.":14672,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y25_04.raw","File.ID":"F4","Quan.Info":"","Peptides.Matched":2163,"XCorr":4.9,"X..Protein.Groups":1,"Percolator.q.Value":0.00016,"Percolator.PEP":2.591e-08,"Percolator.SVMScore":2.915,"Precursor.Area":4244684,"Apex.RT..min.":15.09,"ptmRS..Binomial.Peptide.Score":341.39,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(14): 1621.73; b2+-H(3) O(4) P(18): 1014.90; y2+-H(3) O(4) P(14): 811.37; b1+(12): 1344.56","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AG4)","Search.ID":"AG","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":5,"DeltaScore":0.1018,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40454,"Contaminant":"False","Aligned.m.z..Da.":967.40468,"MH...Da.":2900.19907,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":-0.09,"Deltam.z..Da.":-0.00009,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1754400.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":14.50159,"Ion.Inject.Time..ms.":22,"RT..min.":15.1289,"First.Scan":14772,"Last.Scan":14772,"Master.Scan.s.":14763,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y25_04.raw","File.ID":"F4","Quan.Info":"","Peptides.Matched":2503,"XCorr":6.09,"X..Protein.Groups":1,"Percolator.q.Value":0.00016,"Percolator.PEP":2.17e-13,"Percolator.SVMScore":5.055,"Precursor.Area":4244684,"Apex.RT..min.":15.09,"ptmRS..Binomial.Peptide.Score":601.81,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(13): 1413.58; b1+-H(3) O(4) P(16): 1771.69; y1+-H(3) O(4) P(15): 1750.78; y1+-H(3) O(4) P(14): 1621.73; b2+-H(3) O(4) P(16): 886.35; b2+-H(3) O(4) P(18): 1014.90; y2+-H(3) O(4) P(14): 811.37; b1+(12): 1344.56; y1+(13): 1556.65","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AG4)","Search.ID":"AG","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MGSINNNFNTNNNSNTDLDRDWK","Annotated.Sequence":"[-].mGSINNNFNTNNNsNTDLDRDWK.[T]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S14(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P39517","Master.Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","Protein.Accessions":"P39517","Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.1005,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":892.37203,"Contaminant":"False","Aligned.m.z..Da.":892.37219,"MH...Da.":2675.10154,"Theo..MH...Da.":2675.11179,"DeltaM..ppm.":-3.83,"Deltam.z..Da.":-0.00342,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":362031.90625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":20.2357,"Ion.Inject.Time..ms.":22,"RT..min.":13.5524,"First.Scan":12943,"Last.Scan":12943,"Master.Scan.s.":12937,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y25_04.raw","File.ID":"F4","Quan.Info":"","Peptides.Matched":2002,"XCorr":4.08,"X..Protein.Groups":1,"Percolator.q.Value":0.00016,"Percolator.PEP":3.119e-08,"Percolator.SVMScore":2.881,"Precursor.Area":566633.5,"Apex.RT..min.":13.57,"ptmRS..Binomial.Peptide.Score":405.14,"ptmRS..Isoform.Confidence.Probability":0.994,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(12): 1459.66; y1+-H(3) O(4) P(10): 1231.57; y2+-H(3) O(4) P(19): 1139.50; y2+-H(3) O(4) P(15): 894.90; y2+-H(3) O(4) P(14): 837.88; y2+-H(3) O(4) P(12): 730.33; y2+-H(3) O(4) P(11): 673.31; y2+-H(3) O(4) P(10): 616.29; y1+(9): 1162.55","ptmRS..Best.Site.Probabilities":"S14(Phospho): 99.35","ptmRS..Phospho.Site.Probabilities":"S(3): 0.0; T(10): 0.0; S(14): 99.4; T(16): 0.7"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AG4)","Search.ID":"AG","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MGSINNNFNTNNNSNTDLDRDWK","Annotated.Sequence":"[-].mGSINNNFNTNNNSNtDLDRDWK.[T]","Modifications":"N-Term(Prot)(Met-loss); T16(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P39517","Master.Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","Protein.Accessions":"P39517","Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.1354,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":878.37107,"Contaminant":"False","Aligned.m.z..Da.":878.37108,"MH...Da.":2633.09866,"Theo..MH...Da.":2633.10123,"DeltaM..ppm.":-0.98,"Deltam.z..Da.":-0.00086,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":592184.625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":7.90093,"Ion.Inject.Time..ms.":22,"RT..min.":11.3469,"First.Scan":10119,"Last.Scan":10119,"Master.Scan.s.":10111,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y25_04.raw","File.ID":"F4","Quan.Info":"","Peptides.Matched":2213,"XCorr":2.88,"X..Protein.Groups":1,"Percolator.q.Value":0.00016,"Percolator.PEP":0.00002,"Percolator.SVMScore":1.724,"Precursor.Area":457336.5625,"Apex.RT..min.":11.35,"ptmRS..Binomial.Peptide.Score":334.62,"ptmRS..Isoform.Confidence.Probability":0.009,"ptmRS..Isoform.Group.Report":"y2+-H(3) O(4) P(18): 1082.48; y2+-H(3) O(4) P(16): 968.43; y2+-H(3) O(4) P(15): 894.90; y2+-H(3) O(4) P(14): 837.88; y2+-H(3) O(4) P(12): 730.33; y2+-H(3) O(4) P(11): 673.31; y2+-H(3) O(4) P(10): 616.29; y1+(9): 1162.55","ptmRS..Best.Site.Probabilities":"S14(Phospho): 99.08","ptmRS..Phospho.Site.Probabilities":"S(3): 0.0; T(10): 0.0; S(14): 99.1; T(16): 0.9"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AG4)","Search.ID":"AG","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSSPLHRLSPTGK","Annotated.Sequence":"[R].mSsPLHRLsPTGK.[S]","Modifications":"M1(Oxidation); S3(Phospho); S9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.4788,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":529.56425,"Contaminant":"False","Aligned.m.z..Da.":529.56431,"MH...Da.":1586.6782,"Theo..MH...Da.":1586.67977,"DeltaM..ppm.":-0.99,"Deltam.z..Da.":-0.00052,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1301213.625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":24.64263,"Ion.Inject.Time..ms.":22,"RT..min.":7.7451,"First.Scan":5468,"Last.Scan":5468,"Master.Scan.s.":5457,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y25_04.raw","File.ID":"F4","Quan.Info":"","Peptides.Matched":978,"XCorr":3.07,"X..Protein.Groups":1,"Percolator.q.Value":0.00016,"Percolator.PEP":0.00043,"Percolator.SVMScore":1.135,"ptmRS..Binomial.Peptide.Score":200.67,"ptmRS..Isoform.Confidence.Probability":0.988,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(8): 877.50; y2+-H(3) O(4) P(12): 671.34; y2+-H(3) O(4) P(10): 544.32; y2+-H(3) O(4) P(8): 439.25; y1+(4): 402.23","ptmRS..Best.Site.Probabilities":"S3(Phospho): 100; S9(Phospho): 98.84","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(3): 100.0; S(9): 98.8; T(11): 1.2"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AG4)","Search.ID":"AG","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"HSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[R].HSDSYsENETNHTNVPISSTGGTNNK.[T]","Modifications":"S6(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":0,"Charge":4,"Original.Precursor.Charge":4,"DeltaScore":0.0341,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":718.30112,"Contaminant":"False","Aligned.m.z..Da.":718.30119,"MH...Da.":2870.18268,"Theo..MH...Da.":2870.18608,"DeltaM..ppm.":-1.19,"Deltam.z..Da.":-0.00085,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1080269,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":7.6569,"First.Scan":5333,"Last.Scan":5333,"Master.Scan.s.":5327,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y25_04.raw","File.ID":"F4","Quan.Info":"","Peptides.Matched":2353,"XCorr":4.4,"X..Protein.Groups":1,"Percolator.q.Value":0.00016,"Percolator.PEP":2.485e-06,"Percolator.SVMScore":2.079,"Precursor.Area":883910.4375,"Apex.RT..min.":7.65,"ptmRS..Binomial.Peptide.Score":356.92,"ptmRS..Isoform.Confidence.Probability":0.973,"ptmRS..Isoform.Group.Report":"b2+-H(3) O(4) P(8): 451.67; b2+-H(3) O(4) P(14): 799.81; b2+-H(3) O(4) P(15): 849.34; b2+-H(3) O(4) P(17): 954.41; b1+(4): 427.16","ptmRS..Best.Site.Probabilities":"S6(Phospho): 97.32","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(4): 0.9; Y(5): 0.0; S(6): 97.3; T(10): 0.9; T(13): 0.9; S(18): 0.0; S(19): 0.0; T(20): 0.0; T(23): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AG4)","Search.ID":"AG","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"IQDPQMTGYVSTR","Annotated.Sequence":"[R].IQDPQmTGyVSTR.[Y]","Modifications":"M6(Oxidation); Y9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P32485","Master.Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","Protein.Accessions":"P32485","Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1525,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":796.34551,"Contaminant":"False","Aligned.m.z..Da.":796.34559,"MH...Da.":1591.68375,"Theo..MH...Da.":1591.6822,"DeltaM..ppm.":0.98,"Deltam.z..Da.":0.00078,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1196716,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":7.684,"First.Scan":5375,"Last.Scan":5375,"Master.Scan.s.":5366,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y25_04.raw","File.ID":"F4","Quan.Info":"","Peptides.Matched":1108,"XCorr":2.82,"X..Protein.Groups":1,"Percolator.q.Value":0.00016,"Percolator.PEP":3.367e-06,"Percolator.SVMScore":2.024,"Precursor.Area":944882.75,"Apex.RT..min.":7.68,"ptmRS..Binomial.Peptide.Score":251.73,"ptmRS..Isoform.Confidence.Probability":0.995,"ptmRS..Isoform.Group.Report":"y1+(6): 762.32","ptmRS..Best.Site.Probabilities":"Y9(Phospho): 99.54","ptmRS..Phospho.Site.Probabilities":"T(7): 0.5; Y(9): 99.5; S(11): 0.0; T(12): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AG4)","Search.ID":"AG","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].msEGITDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S2(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0616,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":731.31494,"Contaminant":"False","Aligned.m.z..Da.":731.31501,"MH...Da.":2191.93029,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":1.12,"Deltam.z..Da.":0.00082,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":346302.28125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":7.58345,"Ion.Inject.Time..ms.":22,"RT..min.":15.7653,"First.Scan":15508,"Last.Scan":15508,"Master.Scan.s.":15505,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y25_04.raw","File.ID":"F4","Quan.Info":"","Peptides.Matched":2084,"XCorr":3.57,"X..Protein.Groups":1,"Percolator.q.Value":0.00016,"Percolator.PEP":2.747e-06,"Percolator.SVMScore":2.061,"Precursor.Area":377425.21875,"Apex.RT..min.":15.78,"ptmRS..Binomial.Peptide.Score":388.19,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(2): 112.04; b1+-H(3) O(4) P(3): 241.08; b1+-H(3) O(4) P(4): 298.10; b1+-H(3) O(4) P(5): 411.19; b1+-H(3) O(4) P(6): 512.24; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+-H(3) O(4) P(9): 869.39","ptmRS..Best.Site.Probabilities":"S2(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 100.0; T(6): 0.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AG4)","Search.ID":"AG","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].msEGITDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S2(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1157,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1096.46836,"Contaminant":"False","Aligned.m.z..Da.":1096.46846,"MH...Da.":2191.92946,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":0.74,"Deltam.z..Da.":0.00081,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":240954.15625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":4.96506,"Ion.Inject.Time..ms.":22,"RT..min.":15.7403,"First.Scan":15482,"Last.Scan":15482,"Master.Scan.s.":15478,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y25_04.raw","File.ID":"F4","Quan.Info":"","Peptides.Matched":2010,"XCorr":3.63,"X..Protein.Groups":1,"Percolator.q.Value":0.00016,"Percolator.PEP":3.492e-08,"Percolator.SVMScore":2.86,"Precursor.Area":2539535,"Apex.RT..min.":15.78,"ptmRS..Binomial.Peptide.Score":496.66,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(2): 112.04; b1+-H(3) O(4) P(3): 241.08; b1+-H(3) O(4) P(4): 298.10; b1+-H(3) O(4) P(5): 411.19; b1+-H(3) O(4) P(6): 512.24; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+-H(3) O(4) P(9): 869.39; b1+-H(3) O(4) P(10): 998.43; y1+(14): 1683.77","ptmRS..Best.Site.Probabilities":"S2(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 100.0; T(6): 0.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AG4)","Search.ID":"AG","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":4,"DeltaScore":0.0639,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40681,"Contaminant":"False","Aligned.m.z..Da.":967.40694,"MH...Da.":2900.20587,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":2.26,"Deltam.z..Da.":0.00218,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":714432,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":64.05521,"Ion.Inject.Time..ms.":22,"RT..min.":15.1696,"First.Scan":14823,"Last.Scan":14823,"Master.Scan.s.":14815,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y25_04.raw","File.ID":"F4","Quan.Info":"","Peptides.Matched":2541,"XCorr":3.91,"X..Protein.Groups":1,"Percolator.q.Value":0.00016,"Percolator.PEP":7.638e-08,"Percolator.SVMScore":2.717,"Precursor.Area":4244684,"Apex.RT..min.":15.09,"ptmRS..Binomial.Peptide.Score":358.91,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(14): 1621.73; b2+-H(3) O(4) P(16): 886.35; b2+-H(3) O(4) P(18): 1014.90; y2+-H(3) O(4) P(14): 811.37; b1+(12): 1344.56","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AG4)","Search.ID":"AG","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.3865,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40189,"Contaminant":"False","Aligned.m.z..Da.":967.402,"MH...Da.":2900.19114,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":-2.82,"Deltam.z..Da.":-0.00273,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":153848.71875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":19.85037,"Ion.Inject.Time..ms.":22,"RT..min.":15.5622,"First.Scan":15290,"Last.Scan":15290,"Master.Scan.s.":15289,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y25_04.raw","File.ID":"F4","Quan.Info":"","Peptides.Matched":2141,"XCorr":3.26,"X..Protein.Groups":1,"Percolator.q.Value":0.00016,"Percolator.PEP":0.00001,"Percolator.SVMScore":1.775,"Precursor.Area":1044471.25,"Apex.RT..min.":15.36,"ptmRS..Binomial.Peptide.Score":274.49,"ptmRS..Isoform.Confidence.Probability":0.5,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"Y12(Phospho): 50; S13(Phospho): 50","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 50.0; S(13): 50.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AG4)","Search.ID":"AG","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DIFSNDELLSDAYDAK","Annotated.Sequence":"[K].DIFSNDELLSDAYDAK.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.734,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":908.41653,"Contaminant":"False","Aligned.m.z..Da.":908.41648,"MH...Da.":1815.82579,"Theo..MH...Da.":1815.82831,"DeltaM..ppm.":-1.39,"Deltam.z..Da.":-0.00126,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":282255.90625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":25.11028,"Ion.Inject.Time..ms.":22,"RT..min.":17.0706,"First.Scan":16946,"Last.Scan":16946,"Master.Scan.s.":16941,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y25_04.raw","File.ID":"F4","Quan.Info":"","Peptides.Matched":1551,"XCorr":2.97,"X..Protein.Groups":1,"Percolator.q.Value":0.00016,"Percolator.PEP":4.35e-07,"Percolator.SVMScore":2.398,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (S4)","Search.ID":"S","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"GVFGYGFEEPSAIQQR","Annotated.Sequence":"[R].GVFGYGFEEPSAIQQR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6569,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":892.93446,"Contaminant":"False","Aligned.m.z..Da.":892.93454,"MH...Da.":1784.86164,"Theo..MH...Da.":1784.86022,"DeltaM..ppm.":0.8,"Deltam.z..Da.":0.00071,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":229152.32812,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":15.0348,"First.Scan":15124,"Last.Scan":15124,"Master.Scan.s.":15123,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_06.raw","File.ID":"F18","Quan.Info":"","Peptides.Matched":1421,"XCorr":3.76,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":2.92e-09,"Percolator.SVMScore":3.547,"Precursor.Area":278006.625,"Apex.RT..min.":15.05,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (S4)","Search.ID":"S","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0266,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40697,"Contaminant":"False","Aligned.m.z..Da.":967.40695,"MH...Da.":2900.20636,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":2.42,"Deltam.z..Da.":0.00234,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":271164.90625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":15.4584,"First.Scan":15612,"Last.Scan":15612,"Master.Scan.s.":15610,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_06.raw","File.ID":"F18","Quan.Info":"","Peptides.Matched":2595,"XCorr":4.89,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":3.767e-07,"Percolator.SVMScore":2.58,"Precursor.Area":8694983,"Apex.RT..min.":15.02,"ptmRS..Binomial.Peptide.Score":312.97,"ptmRS..Isoform.Confidence.Probability":0.5,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"Y12(Phospho): 50; S13(Phospho): 50","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 50.0; S(13): 50.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (S4)","Search.ID":"S","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].msEGITDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S2(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0647,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":731.3132,"Contaminant":"False","Aligned.m.z..Da.":731.31316,"MH...Da.":2191.92506,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":-1.27,"Deltam.z..Da.":-0.00093,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":257515.28125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":4.47456,"Ion.Inject.Time..ms.":22,"RT..min.":15.678,"First.Scan":15816,"Last.Scan":15816,"Master.Scan.s.":15813,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_06.raw","File.ID":"F18","Quan.Info":"","Peptides.Matched":1907,"XCorr":3.71,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":6.33e-06,"Percolator.SVMScore":2.018,"Precursor.Area":450236.9375,"Apex.RT..min.":15.7,"ptmRS..Binomial.Peptide.Score":362.17,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(2): 112.04; b1+-H(3) O(4) P(3): 241.08; b1+-H(3) O(4) P(4): 298.10; b1+-H(3) O(4) P(5): 411.19; b1+-H(3) O(4) P(6): 512.24; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+-H(3) O(4) P(9): 869.39; b1+-H(3) O(4) P(10): 998.43","ptmRS..Best.Site.Probabilities":"S2(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 100.0; T(6): 0.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (S4)","Search.ID":"S","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.3967,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40696,"Contaminant":"False","Aligned.m.z..Da.":967.40692,"MH...Da.":2900.20633,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":2.41,"Deltam.z..Da.":0.00233,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":362807.84375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":38.28429,"Ion.Inject.Time..ms.":22,"RT..min.":15.5787,"First.Scan":15698,"Last.Scan":15698,"Master.Scan.s.":15694,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_06.raw","File.ID":"F18","Quan.Info":"","Peptides.Matched":2595,"XCorr":4.21,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":1.174e-08,"Percolator.SVMScore":3.27,"ptmRS..Binomial.Peptide.Score":390.24,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(14): 1621.73; b2+-H(3) O(4) P(18): 1014.90; y2+-H(3) O(4) P(14): 811.37","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (S4)","Search.ID":"S","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":4,"DeltaScore":0.0601,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40492,"Contaminant":"False","Aligned.m.z..Da.":967.40498,"MH...Da.":2900.20021,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":0.3,"Deltam.z..Da.":0.00029,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1374150.375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":10.06368,"Ion.Inject.Time..ms.":22,"RT..min.":15.1264,"First.Scan":15229,"Last.Scan":15229,"Master.Scan.s.":15217,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_06.raw","File.ID":"F18","Quan.Info":"","Peptides.Matched":2490,"XCorr":4.99,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":7.136e-10,"Percolator.SVMScore":3.828,"Precursor.Area":8694983,"Apex.RT..min.":15.02,"ptmRS..Binomial.Peptide.Score":356.45,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(13): 1458.67; b1+(12): 1344.56","ptmRS..Best.Site.Probabilities":"S13(Phospho): 99.99","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (S4)","Search.ID":"S","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":5,"DeltaScore":0.1105,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40249,"Contaminant":"False","Aligned.m.z..Da.":967.40259,"MH...Da.":2900.19292,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":-2.21,"Deltam.z..Da.":-0.00214,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1470157.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":43.26615,"Ion.Inject.Time..ms.":22,"RT..min.":14.9767,"First.Scan":15057,"Last.Scan":15057,"Master.Scan.s.":15054,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_06.raw","File.ID":"F18","Quan.Info":"","Peptides.Matched":2156,"XCorr":5.88,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":1.711e-10,"Percolator.SVMScore":4.112,"Precursor.Area":8694983,"Apex.RT..min.":15.02,"ptmRS..Binomial.Peptide.Score":517.27,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(13): 1413.58; y1+-H(3) O(4) P(14): 1621.73; b2+-H(3) O(4) P(16): 886.35; b2+-H(3) O(4) P(17): 950.38; b2+-H(3) O(4) P(18): 1014.90; b2+-H(3) O(4) P(19): 1071.44; y2+-H(3) O(4) P(14): 811.37; b1+(12): 1344.56; y1+(13): 1556.65","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (S4)","Search.ID":"S","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FTVSAIYSDLPQQER","Annotated.Sequence":"[K].FTVSAIYSDLPQQER.[D]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6917,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":877.44079,"Contaminant":"False","Aligned.m.z..Da.":877.44088,"MH...Da.":1753.8743,"Theo..MH...Da.":1753.87554,"DeltaM..ppm.":-0.71,"Deltam.z..Da.":-0.00062,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":579228.5625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":10.33382,"Ion.Inject.Time..ms.":22,"RT..min.":14.9869,"First.Scan":15070,"Last.Scan":15070,"Master.Scan.s.":15067,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_06.raw","File.ID":"F18","Quan.Info":"","Peptides.Matched":1170,"XCorr":3.73,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":3.991e-09,"Percolator.SVMScore":3.485,"Precursor.Area":374238.09375,"Apex.RT..min.":14.98,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (S4)","Search.ID":"S","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":4,"DeltaScore":0.0607,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40474,"Contaminant":"False","Aligned.m.z..Da.":967.4048,"MH...Da.":2900.19966,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":0.12,"Deltam.z..Da.":0.00011,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1694850.375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":33.34673,"Ion.Inject.Time..ms.":22,"RT..min.":15.1038,"First.Scan":15200,"Last.Scan":15200,"Master.Scan.s.":15191,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_06.raw","File.ID":"F18","Quan.Info":"","Peptides.Matched":2465,"XCorr":5.27,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":2.167e-10,"Percolator.SVMScore":4.065,"Precursor.Area":8694983,"Apex.RT..min.":15.02,"ptmRS..Binomial.Peptide.Score":549.03,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(13): 1413.58; b1+-H(3) O(4) P(14): 1528.61; b1+-H(3) O(4) P(16): 1771.69; y1+-H(3) O(4) P(15): 1750.78; y1+-H(3) O(4) P(14): 1621.73; b2+-H(3) O(4) P(16): 886.35; b2+-H(3) O(4) P(18): 1014.90; y2+-H(3) O(4) P(14): 811.37; b1+(12): 1344.56","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (S4)","Search.ID":"S","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0619,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.4078,"Contaminant":"False","Aligned.m.z..Da.":967.40791,"MH...Da.":2900.20885,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":3.28,"Deltam.z..Da.":0.00317,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1162103.125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":43.86687,"Ion.Inject.Time..ms.":22,"RT..min.":14.9551,"First.Scan":15029,"Last.Scan":15029,"Master.Scan.s.":15028,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_06.raw","File.ID":"F18","Quan.Info":"","Peptides.Matched":2629,"XCorr":4.04,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":3.964e-08,"Percolator.SVMScore":3.028,"ptmRS..Binomial.Peptide.Score":341.98,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b2+-H(3) O(4) P(16): 886.35; y2+-H(3) O(4) P(14): 811.37; b1+(12): 1344.56","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (S4)","Search.ID":"S","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.4534,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40379,"Contaminant":"False","Aligned.m.z..Da.":967.40376,"MH...Da.":2900.19682,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":-0.87,"Deltam.z..Da.":-0.00084,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":214470.46875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":14.46985,"Ion.Inject.Time..ms.":22,"RT..min.":16.0961,"First.Scan":16214,"Last.Scan":16214,"Master.Scan.s.":16212,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_06.raw","File.ID":"F18","Quan.Info":"","Peptides.Matched":2361,"XCorr":3.86,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":1.385e-06,"Percolator.SVMScore":2.32,"Precursor.Area":429217.5,"Apex.RT..min.":16.04,"ptmRS..Binomial.Peptide.Score":286.39,"ptmRS..Isoform.Confidence.Probability":0.5,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"Y12(Phospho): 50; S13(Phospho): 50","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 50.0; S(13): 50.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (S4)","Search.ID":"S","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].msEGITDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S2(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":3,"DeltaScore":0.187,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1096.46763,"Contaminant":"False","Aligned.m.z..Da.":1096.46757,"MH...Da.":2191.92799,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":0.07,"Deltam.z..Da.":0.00007,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":423691.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":62.55585,"Ion.Inject.Time..ms.":22,"RT..min.":15.805,"First.Scan":15912,"Last.Scan":15912,"Master.Scan.s.":15911,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_06.raw","File.ID":"F18","Quan.Info":"","Peptides.Matched":1955,"XCorr":2.46,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":0.00108,"Percolator.SVMScore":0.993,"Precursor.Area":3811862.75,"Apex.RT..min.":15.7,"ptmRS..Binomial.Peptide.Score":191.58,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(2): 112.04; b1+-H(3) O(4) P(3): 241.08; b1+-H(3) O(4) P(4): 298.10; b1+-H(3) O(4) P(5): 411.19; b1+-H(3) O(4) P(6): 512.24; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35","ptmRS..Best.Site.Probabilities":"S2(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 100.0; T(6): 0.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (S4)","Search.ID":"S","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].msEGITDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S2(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":3,"DeltaScore":0.1707,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1096.46458,"Contaminant":"False","Aligned.m.z..Da.":1096.46452,"MH...Da.":2191.92188,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":-2.72,"Deltam.z..Da.":-0.00298,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":182191.35938,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":83.13701,"Ion.Inject.Time..ms.":22,"RT..min.":15.8899,"First.Scan":15998,"Last.Scan":15998,"Master.Scan.s.":15995,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_06.raw","File.ID":"F18","Quan.Info":"","Peptides.Matched":1839,"XCorr":2.05,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":0.00125,"Percolator.SVMScore":0.965,"Precursor.Area":3811862.75,"Apex.RT..min.":15.7,"ptmRS..Binomial.Peptide.Score":248.22,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(2): 112.04; b1+-H(3) O(4) P(3): 241.08; b1+-H(3) O(4) P(4): 298.10; b1+-H(3) O(4) P(5): 411.19; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35","ptmRS..Best.Site.Probabilities":"S2(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 100.0; T(6): 0.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (S4)","Search.ID":"S","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DIFSNDELLSDAYDAK","Annotated.Sequence":"[K].DIFSNDELLSDAYDAK.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6667,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":908.42122,"Contaminant":"False","Aligned.m.z..Da.":908.42147,"MH...Da.":1815.83518,"Theo..MH...Da.":1815.82831,"DeltaM..ppm.":3.78,"Deltam.z..Da.":0.00343,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":213901.70312,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":10.9553,"Ion.Inject.Time..ms.":22,"RT..min.":17.0906,"First.Scan":16999,"Last.Scan":16999,"Master.Scan.s.":16990,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_06.raw","File.ID":"F18","Quan.Info":"","Peptides.Matched":1808,"XCorr":3.45,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":2.756e-08,"Percolator.SVMScore":3.1,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (S4)","Search.ID":"S","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DIFSNDELLSDAYDAK","Annotated.Sequence":"[K].DIFSNDELLSDAYDAK.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.7,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":605.94824,"Contaminant":"False","Aligned.m.z..Da.":605.94841,"MH...Da.":1815.83018,"Theo..MH...Da.":1815.82831,"DeltaM..ppm.":1.03,"Deltam.z..Da.":0.00062,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":148637.48438,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":17.1065,"First.Scan":17014,"Last.Scan":17014,"Master.Scan.s.":17003,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_06.raw","File.ID":"F18","Quan.Info":"","Peptides.Matched":1662,"XCorr":2.8,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":0.00012,"Percolator.SVMScore":1.434,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (S4)","Search.ID":"S","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.358,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40564,"Contaminant":"False","Aligned.m.z..Da.":967.40579,"MH...Da.":2900.20237,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":1.05,"Deltam.z..Da.":0.00102,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":222554.59375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":48.30158,"Ion.Inject.Time..ms.":22,"RT..min.":16.6819,"First.Scan":16645,"Last.Scan":16645,"Master.Scan.s.":16643,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_06.raw","File.ID":"F18","Quan.Info":"","Peptides.Matched":2543,"XCorr":4.05,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":4.019e-08,"Percolator.SVMScore":3.025,"Precursor.Area":325738.78125,"Apex.RT..min.":16.71,"ptmRS..Binomial.Peptide.Score":295,"ptmRS..Isoform.Confidence.Probability":0.5,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"Y12(Phospho): 50; S13(Phospho): 50","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 50.0; S(13): 50.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (S4)","Search.ID":"S","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MFILDEADEMLSSGFK","Annotated.Sequence":"[K].mFILDEADEmLSSGFK.[E]","Modifications":"M1(Oxidation); M10(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6643,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":932.92313,"Contaminant":"False","Aligned.m.z..Da.":932.92327,"MH...Da.":1864.83898,"Theo..MH...Da.":1864.83433,"DeltaM..ppm.":2.5,"Deltam.z..Da.":0.00233,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":308961.4375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":5.72792,"Ion.Inject.Time..ms.":22,"RT..min.":16.6873,"First.Scan":16652,"Last.Scan":16652,"Master.Scan.s.":16643,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_06.raw","File.ID":"F18","Quan.Info":"","Peptides.Matched":1868,"XCorr":2.83,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":3.515e-08,"Percolator.SVMScore":3.052,"Precursor.Area":354802.625,"Apex.RT..min.":16.71,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (S4)","Search.ID":"S","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"HSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[R].HSDsYsENETNHTNVPISSTGGTNNK.[T]","Modifications":"S4(Phospho); S6(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.1975,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":984.05831,"Contaminant":"False","Aligned.m.z..Da.":984.05842,"MH...Da.":2950.16039,"Theo..MH...Da.":2950.15241,"DeltaM..ppm.":2.7,"Deltam.z..Da.":0.00266,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1510522.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":8.99656,"Ion.Inject.Time..ms.":22,"RT..min.":7.9531,"First.Scan":6134,"Last.Scan":6134,"Master.Scan.s.":6125,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_06.raw","File.ID":"F18","Quan.Info":"","Peptides.Matched":1265,"XCorr":6.33,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":2.895e-09,"Percolator.SVMScore":3.549,"Precursor.Area":1896724.375,"Apex.RT..min.":7.96,"ptmRS..Binomial.Peptide.Score":532.39,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(6): 739.21; y2+-H(3) O(4) P(23): 1257.03; y2+-H(3) O(4) P(22): 1173.53; y2+-H(3) O(4) P(21): 1092.00; b1+- -2H(3) O(4) P -2H(3) O(4) P(6): 641.23; b1+- -2H(3) O(4) P -2H(3) O(4) P(11): 1228.45; b2+- -2H(3) O(4) P -2H(3) O(4) P(13): 733.78; b2+- -2H(3) O(4) P -2H(3) O(4) P(14): 790.80; b2+- -2H(3) O(4) P -2H(3) O(4) P(15): 840.34","ptmRS..Best.Site.Probabilities":"S4(Phospho): 100; S6(Phospho): 99.99","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(4): 100.0; Y(5): 0.0; S(6): 100.0; T(10): 0.0; T(13): 0.0; S(18): 0.0; S(19): 0.0; T(20): 0.0; T(23): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (S4)","Search.ID":"S","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSSPLHRLSPTGK","Annotated.Sequence":"[R].mSsPLHRLsPTGK.[S]","Modifications":"M1(Oxidation); S3(Phospho); S9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.4937,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":529.56518,"Contaminant":"False","Aligned.m.z..Da.":529.56523,"MH...Da.":1586.68101,"Theo..MH...Da.":1586.67977,"DeltaM..ppm.":0.78,"Deltam.z..Da.":0.00041,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2661251,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":39.75032,"Ion.Inject.Time..ms.":22,"RT..min.":7.7033,"First.Scan":5803,"Last.Scan":5803,"Master.Scan.s.":5800,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_06.raw","File.ID":"F18","Quan.Info":"","Peptides.Matched":1071,"XCorr":3.16,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":0.00008,"Percolator.SVMScore":1.514,"ptmRS..Binomial.Peptide.Score":228.17,"ptmRS..Isoform.Confidence.Probability":0.991,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(8): 877.50; y2+-H(3) O(4) P(11): 627.82; y2+-H(3) O(4) P(10): 544.32; y2+-H(3) O(4) P(8): 439.25; y1+(4): 402.23","ptmRS..Best.Site.Probabilities":"S3(Phospho): 100; S9(Phospho): 99.06","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(3): 100.0; S(9): 99.1; T(11): 0.9"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (S4)","Search.ID":"S","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LQETNPEEVPK","Annotated.Sequence":"[K].LQETNPEEVPK.[F]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":3,"DeltaScore":0.4813,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":642.32765,"Contaminant":"False","Aligned.m.z..Da.":642.3277,"MH...Da.":1283.64802,"Theo..MH...Da.":1283.64777,"DeltaM..ppm.":0.19,"Deltam.z..Da.":0.00012,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":4065208.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":32.76914,"Ion.Inject.Time..ms.":22,"RT..min.":7.6693,"First.Scan":5760,"Last.Scan":5760,"Master.Scan.s.":5748,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_06.raw","File.ID":"F18","Quan.Info":"","Peptides.Matched":526,"XCorr":1.87,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":0.00021,"Percolator.SVMScore":1.319,"Precursor.Area":4071407.75,"Apex.RT..min.":7.65,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (S4)","Search.ID":"S","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"HSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[R].HSDSYsENETNHTNVPISSTGGTNNK.[T]","Modifications":"S6(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.005,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":957.40001,"Contaminant":"False","Aligned.m.z..Da.":957.40007,"MH...Da.":2870.18548,"Theo..MH...Da.":2870.18608,"DeltaM..ppm.":-0.21,"Deltam.z..Da.":-0.0002,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1698121.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":7.5157,"First.Scan":5558,"Last.Scan":5558,"Master.Scan.s.":5553,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_06.raw","File.ID":"F18","Quan.Info":"","Peptides.Matched":2441,"XCorr":5.99,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":1.981e-15,"Percolator.SVMScore":6.611,"Precursor.Area":6691683.5,"Apex.RT..min.":7.53,"ptmRS..Binomial.Peptide.Score":518.9,"ptmRS..Isoform.Confidence.Probability":0.006,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(4): 409.15; b1+-H(3) O(4) P(5): 572.21; b1+-H(3) O(4) P(6): 659.24; b1+-H(3) O(4) P(10): 1132.42; b1+-H(3) O(4) P(11): 1246.46; b1+-H(3) O(4) P(15): 1697.68; b2+-H(3) O(4) P(12): 692.26; b2+-H(3) O(4) P(13): 742.79; b2+-H(3) O(4) P(14): 799.81; b2+-H(3) O(4) P(15): 849.34; y2+-H(3) O(4) P(25): 1318.08; y2+-H(3) O(4) P(24): 1274.56; y2+-H(3) O(4) P(23): 1217.05","ptmRS..Best.Site.Probabilities":"S4(Phospho): 99.35","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(4): 99.4; Y(5): 0.0; S(6): 0.6; T(10): 0.0; T(13): 0.0; S(18): 0.0; S(19): 0.0; T(20): 0.0; T(23): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (S4)","Search.ID":"S","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"HSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[R].HSDSYsENETNHTNVPISSTGGTNNK.[T]","Modifications":"S6(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":0,"Charge":4,"Original.Precursor.Charge":4,"DeltaScore":0.0041,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":718.30153,"Contaminant":"False","Aligned.m.z..Da.":718.30157,"MH...Da.":2870.1843,"Theo..MH...Da.":2870.18608,"DeltaM..ppm.":-0.62,"Deltam.z..Da.":-0.00045,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3842820,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":62.68911,"Ion.Inject.Time..ms.":6.842,"RT..min.":7.5243,"First.Scan":5569,"Last.Scan":5569,"Master.Scan.s.":5566,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_06.raw","File.ID":"F18","Quan.Info":"","Peptides.Matched":2461,"XCorr":2.42,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":0.00001,"Percolator.SVMScore":1.859,"Precursor.Area":3732950,"Apex.RT..min.":7.53,"ptmRS..Binomial.Peptide.Score":308.48,"ptmRS..Isoform.Confidence.Probability":0.496,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(11): 1246.46; b2+-H(3) O(4) P(14): 799.81; b2+-H(3) O(4) P(15): 849.34; b2+-H(3) O(4) P(17): 954.41","ptmRS..Best.Site.Probabilities":"S6(Phospho): 49.62; T10(Phospho): 49.62","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(4): 0.4; Y(5): 0.0; S(6): 49.6; T(10): 49.6; T(13): 0.4; S(18): 0.0; S(19): 0.0; T(20): 0.0; T(23): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (S4)","Search.ID":"S","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LQETNPEEVPK","Annotated.Sequence":"[K].LQETNPEEVPK.[F]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5056,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":642.32766,"Contaminant":"False","Aligned.m.z..Da.":642.3277,"MH...Da.":1283.64804,"Theo..MH...Da.":1283.64777,"DeltaM..ppm.":0.21,"Deltam.z..Da.":0.00013,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3320078.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":18.96971,"Ion.Inject.Time..ms.":22,"RT..min.":7.6342,"First.Scan":5712,"Last.Scan":5712,"Master.Scan.s.":5709,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_06.raw","File.ID":"F18","Quan.Info":"","Peptides.Matched":526,"XCorr":2.67,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":0.00039,"Percolator.SVMScore":1.198,"Precursor.Area":4071407.75,"Apex.RT..min.":7.65,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (S4)","Search.ID":"S","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"IQDPQMTGYVSTR","Annotated.Sequence":"[R].IQDPQmTGyVSTR.[Y]","Modifications":"M6(Oxidation); Y9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P32485","Master.Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","Protein.Accessions":"P32485","Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1386,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":796.34637,"Contaminant":"False","Aligned.m.z..Da.":796.34643,"MH...Da.":1591.68548,"Theo..MH...Da.":1591.6822,"DeltaM..ppm.":2.06,"Deltam.z..Da.":0.00164,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1513799.875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":5.01715,"Ion.Inject.Time..ms.":22,"RT..min.":7.605,"First.Scan":5674,"Last.Scan":5674,"Master.Scan.s.":5670,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_06.raw","File.ID":"F18","Quan.Info":"","Peptides.Matched":1170,"XCorr":3.03,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":1.417e-06,"Percolator.SVMScore":2.316,"Precursor.Area":3888355.75,"Apex.RT..min.":7.64,"ptmRS..Binomial.Peptide.Score":263.02,"ptmRS..Isoform.Confidence.Probability":0.99,"ptmRS..Isoform.Group.Report":"y1+(6): 762.32; y1+(5): 705.30","ptmRS..Best.Site.Probabilities":"Y9(Phospho): 98.97","ptmRS..Phospho.Site.Probabilities":"T(7): 0.5; Y(9): 99.0; S(11): 0.5; T(12): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (S4)","Search.ID":"S","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LQETNPEEVPKFEK","Annotated.Sequence":"[K].LQETNPEEVPKFEK.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.5672,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":563.29065,"Contaminant":"False","Aligned.m.z..Da.":563.29061,"MH...Da.":1687.85742,"Theo..MH...Da.":1687.85374,"DeltaM..ppm.":2.18,"Deltam.z..Da.":0.00122,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1376958.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":75.14671,"Ion.Inject.Time..ms.":15.794,"RT..min.":9.2998,"First.Scan":7950,"Last.Scan":7950,"Master.Scan.s.":7945,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_06.raw","File.ID":"F18","Quan.Info":"","Peptides.Matched":860,"XCorr":2.01,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":0.00021,"Percolator.SVMScore":1.319,"Precursor.Area":943086,"Apex.RT..min.":9.3,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (S4)","Search.ID":"S","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DFRHSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[K].DFRHSDsYsENETNHTNVPISSTGGTNNK.[T]","Modifications":"S7(Phospho); S9(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":1,"Charge":4,"Original.Precursor.Charge":4,"DeltaScore":0.0395,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":842.843,"Contaminant":"False","Aligned.m.z..Da.":842.84305,"MH...Da.":3368.35017,"Theo..MH...Da.":3368.34888,"DeltaM..ppm.":0.38,"Deltam.z..Da.":0.00032,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1433550.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":8.5937,"First.Scan":6985,"Last.Scan":6985,"Master.Scan.s.":6983,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_06.raw","File.ID":"F18","Quan.Info":"","Peptides.Matched":951,"XCorr":6.08,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":1.612e-08,"Percolator.SVMScore":3.207,"Precursor.Area":1265999.625,"Apex.RT..min.":8.59,"ptmRS..Binomial.Peptide.Score":363.24,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"b2+-H(3) O(4) P(7): 454.16; b2+-H(3) O(4) P(8): 535.69; b2+- -2H(3) O(4) P -2H(3) O(4) P(15): 892.36; b2+- -2H(3) O(4) P -2H(3) O(4) P(17): 999.90; b2+- -2H(3) O(4) P -2H(3) O(4) P(18): 1049.44","ptmRS..Best.Site.Probabilities":"S5(Phospho): 99.98; S7(Phospho): 98.59","ptmRS..Phospho.Site.Probabilities":"S(5): 100.0; S(7): 98.6; Y(8): 0.0; S(9): 1.4; T(13): 0.0; T(16): 0.0; S(21): 0.0; S(22): 0.0; T(23): 0.0; T(26): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (S4)","Search.ID":"S","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"AAEAGETGAATSATEGDNNNNTAAGDKK","Annotated.Sequence":"[K].AAEAGETGAATsATEGDNNNNTAAGDKK.[G]","Modifications":"S12(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0475,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":906.04904,"Contaminant":"False","Aligned.m.z..Da.":906.0491,"MH...Da.":2716.13258,"Theo..MH...Da.":2716.13298,"DeltaM..ppm.":-0.15,"Deltam.z..Da.":-0.00013,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3469168.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":2.29444,"Ion.Inject.Time..ms.":22,"RT..min.":5.1185,"First.Scan":2087,"Last.Scan":2087,"Master.Scan.s.":2082,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_06.raw","File.ID":"F18","Quan.Info":"","Peptides.Matched":2549,"XCorr":6.32,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":7.086e-14,"Percolator.SVMScore":5.663,"ptmRS..Binomial.Peptide.Score":483.6,"ptmRS..Isoform.Confidence.Probability":0.992,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(17): 1688.75; y2+-H(3) O(4) P(22): 1045.47; y2+-H(3) O(4) P(19): 930.92; y2+-H(3) O(4) P(18): 895.40; y2+-H(3) O(4) P(17): 844.88; y2+(21): 1043.94","ptmRS..Best.Site.Probabilities":"S12(Phospho): 99.21","ptmRS..Phospho.Site.Probabilities":"T(7): 0.8; T(11): 0.0; S(12): 99.2; T(14): 0.0; T(22): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (S4)","Search.ID":"S","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KFQDDTLNR","Annotated.Sequence":"[K].KFQDDtLNR.[V]","Modifications":"T6(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6091,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":608.77283,"Contaminant":"False","Aligned.m.z..Da.":608.77282,"MH...Da.":1216.53838,"Theo..MH...Da.":1216.53579,"DeltaM..ppm.":2.13,"Deltam.z..Da.":0.00129,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1182054.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":7.49542,"Ion.Inject.Time..ms.":22,"RT..min.":6.1961,"First.Scan":3588,"Last.Scan":3588,"Master.Scan.s.":3577,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_06.raw","File.ID":"F18","Quan.Info":"","Peptides.Matched":670,"XCorr":2.43,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":0.00056,"Percolator.SVMScore":1.123,"Precursor.Area":1145694.125,"Apex.RT..min.":6.21,"ptmRS..Binomial.Peptide.Score":305.4,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"T6(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"T(6): 100.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (S4)","Search.ID":"S","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"AIMPIIEGHDVLAQAQSGTGK","Annotated.Sequence":"[R].AImPIIEGHDVLAQAQSGTGK.[T]","Modifications":"M3(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.7417,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":718.04232,"Contaminant":"False","Aligned.m.z..Da.":718.04218,"MH...Da.":2152.11241,"Theo..MH...Da.":2152.10668,"DeltaM..ppm.":2.66,"Deltam.z..Da.":0.00191,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":366144.6875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":6.38241,"Ion.Inject.Time..ms.":22,"RT..min.":12.6253,"First.Scan":12421,"Last.Scan":12421,"Master.Scan.s.":12409,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_06.raw","File.ID":"F18","Quan.Info":"","Peptides.Matched":973,"XCorr":3.91,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":6.455e-09,"Percolator.SVMScore":3.389,"Precursor.Area":479499.15625,"Apex.RT..min.":12.65,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (S4)","Search.ID":"S","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].mSEGITDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7219,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1056.48655,"Contaminant":"False","Aligned.m.z..Da.":1056.48675,"MH...Da.":2111.96583,"Theo..MH...Da.":2111.96151,"DeltaM..ppm.":2.05,"Deltam.z..Da.":0.00216,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":327428.34375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":89.17289,"Ion.Inject.Time..ms.":22,"RT..min.":14.6132,"First.Scan":14683,"Last.Scan":14683,"Master.Scan.s.":14678,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_06.raw","File.ID":"F18","Quan.Info":"","Peptides.Matched":2383,"XCorr":3.74,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":5.27e-10,"Percolator.SVMScore":3.888,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (S4)","Search.ID":"S","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FDDMELDENLLR","Annotated.Sequence":"[K].FDDmELDENLLR.[G]","Modifications":"M4(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5892,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":763.34659,"Contaminant":"False","Aligned.m.z..Da.":763.34674,"MH...Da.":1525.6859,"Theo..MH...Da.":1525.6839,"DeltaM..ppm.":1.31,"Deltam.z..Da.":0.001,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":203877.4375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":15.8964,"Ion.Inject.Time..ms.":22,"RT..min.":14.4962,"First.Scan":14590,"Last.Scan":14590,"Master.Scan.s.":14585,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_06.raw","File.ID":"F18","Quan.Info":"","Peptides.Matched":1003,"XCorr":2.97,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":1.323e-06,"Percolator.SVMScore":2.329,"Precursor.Area":110898.3125,"Apex.RT..min.":14.56,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (S4)","Search.ID":"S","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FDDMELDENLLR","Annotated.Sequence":"[K].FDDmELDENLLR.[G]","Modifications":"M4(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":3,"DeltaScore":0.6285,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":763.34701,"Contaminant":"False","Aligned.m.z..Da.":763.34715,"MH...Da.":1525.68673,"Theo..MH...Da.":1525.6839,"DeltaM..ppm.":1.86,"Deltam.z..Da.":0.00142,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":71299.86719,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":75.88554,"Ion.Inject.Time..ms.":22,"RT..min.":14.5794,"First.Scan":14655,"Last.Scan":14655,"Master.Scan.s.":14653,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_06.raw","File.ID":"F18","Quan.Info":"","Peptides.Matched":1016,"XCorr":2.53,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":1.306e-07,"Percolator.SVMScore":2.79,"Precursor.Area":110898.3125,"Apex.RT..min.":14.56,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (S4)","Search.ID":"S","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"TGTFSIAALQR","Annotated.Sequence":"[K].TGTFSIAALQR.[I]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6584,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":582.82196,"Contaminant":"False","Aligned.m.z..Da.":582.82196,"MH...Da.":1164.63665,"Theo..MH...Da.":1164.63715,"DeltaM..ppm.":-0.43,"Deltam.z..Da.":-0.00025,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":518613.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":50.39365,"Ion.Inject.Time..ms.":22,"RT..min.":13.4243,"First.Scan":13458,"Last.Scan":13458,"Master.Scan.s.":13457,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_06.raw","File.ID":"F18","Quan.Info":"","Peptides.Matched":364,"XCorr":2.43,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":7.751e-06,"Percolator.SVMScore":1.977,"Precursor.Area":1391772.125,"Apex.RT..min.":13.4,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (S4)","Search.ID":"S","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"TGTFSIAALQR","Annotated.Sequence":"[K].TGTFSIAALQR.[I]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":3,"DeltaScore":0.5829,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":582.82196,"Contaminant":"False","Aligned.m.z..Da.":582.82196,"MH...Da.":1164.63664,"Theo..MH...Da.":1164.63715,"DeltaM..ppm.":-0.43,"Deltam.z..Da.":-0.00025,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":518613.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":51.95103,"Ion.Inject.Time..ms.":22,"RT..min.":13.4267,"First.Scan":13462,"Last.Scan":13462,"Master.Scan.s.":13457,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_06.raw","File.ID":"F18","Quan.Info":"","Peptides.Matched":364,"XCorr":1.75,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":0.00004,"Percolator.SVMScore":1.639,"Precursor.Area":1391772.125,"Apex.RT..min.":13.4,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (S4)","Search.ID":"S","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"APQALMLAPTR","Annotated.Sequence":"[K].APQALmLAPTR.[E]","Modifications":"M6(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7093,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":592.8263,"Contaminant":"False","Aligned.m.z..Da.":592.82628,"MH...Da.":1184.64532,"Theo..MH...Da.":1184.6456,"DeltaM..ppm.":-0.24,"Deltam.z..Da.":-0.00014,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1034086.8125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":15.28908,"Ion.Inject.Time..ms.":22,"RT..min.":10.2034,"First.Scan":9202,"Last.Scan":9202,"Master.Scan.s.":9193,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_06.raw","File.ID":"F18","Quan.Info":"","Peptides.Matched":323,"XCorr":2.89,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":0.00002,"Percolator.SVMScore":1.784,"Precursor.Area":1292003,"Apex.RT..min.":10.22,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (S4)","Search.ID":"S","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KDELTLEGIK","Annotated.Sequence":"[K].KDELTLEGIK.[Q]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.614,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":382.55195,"Contaminant":"False","Aligned.m.z..Da.":382.55194,"MH...Da.":1145.6413,"Theo..MH...Da.":1145.64123,"DeltaM..ppm.":0.06,"Deltam.z..Da.":0.00002,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1230880.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":2.18956,"Ion.Inject.Time..ms.":22,"RT..min.":10.2756,"First.Scan":9301,"Last.Scan":9301,"Master.Scan.s.":9297,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_06.raw","File.ID":"F18","Quan.Info":"","Peptides.Matched":307,"XCorr":2.85,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":0.00143,"Percolator.SVMScore":0.938,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (S4)","Search.ID":"S","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KDELTLEGIK","Annotated.Sequence":"[K].KDELTLEGIK.[Q]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5095,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":573.32486,"Contaminant":"False","Aligned.m.z..Da.":573.32485,"MH...Da.":1145.64245,"Theo..MH...Da.":1145.64123,"DeltaM..ppm.":1.06,"Deltam.z..Da.":0.00061,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1150290.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":4.26623,"Ion.Inject.Time..ms.":22,"RT..min.":10.2679,"First.Scan":9291,"Last.Scan":9291,"Master.Scan.s.":9284,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_06.raw","File.ID":"F18","Quan.Info":"","Peptides.Matched":301,"XCorr":3.69,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":5.024e-07,"Percolator.SVMScore":2.522,"Precursor.Area":1259027.25,"Apex.RT..min.":10.28,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (S4)","Search.ID":"S","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MGSINNNFNTNNNSNTDLDRDWK","Annotated.Sequence":"[-].mGSINNNFNTNNNsNTDLDRDWK.[T]","Modifications":"N-Term(Prot)(Met-loss); S14(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P39517","Master.Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","Protein.Accessions":"P39517","Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.081,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":878.36976,"Contaminant":"False","Aligned.m.z..Da.":878.3697,"MH...Da.":2633.09474,"Theo..MH...Da.":2633.10123,"DeltaM..ppm.":-2.46,"Deltam.z..Da.":-0.00216,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":653013.625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":11.38289,"Ion.Inject.Time..ms.":22,"RT..min.":11.2435,"First.Scan":10620,"Last.Scan":10620,"Master.Scan.s.":10610,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_06.raw","File.ID":"F18","Quan.Info":"","Peptides.Matched":2114,"XCorr":4.2,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":5.154e-09,"Percolator.SVMScore":3.434,"Precursor.Area":671600.5,"Apex.RT..min.":11.25,"ptmRS..Binomial.Peptide.Score":388.92,"ptmRS..Isoform.Confidence.Probability":0.991,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(11): 1345.61; y1+-H(3) O(4) P(10): 1231.57; y2+-H(3) O(4) P(19): 1139.50; y2+-H(3) O(4) P(16): 968.43; y2+-H(3) O(4) P(15): 894.90; y2+-H(3) O(4) P(14): 837.88; y2+-H(3) O(4) P(12): 730.33; y2+-H(3) O(4) P(10): 616.29; y1+(9): 1162.55","ptmRS..Best.Site.Probabilities":"S14(Phospho): 99.11","ptmRS..Phospho.Site.Probabilities":"S(3): 0.0; T(10): 0.0; S(14): 99.1; T(16): 0.9"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (S4)","Search.ID":"S","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"SSQIHIENESTEDILK","Annotated.Sequence":"[R].SSQIHIENEStEDILK.[I]","Modifications":"T11(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.114,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":641.62976,"Contaminant":"False","Aligned.m.z..Da.":641.62971,"MH...Da.":1922.87474,"Theo..MH...Da.":1922.87429,"DeltaM..ppm.":0.23,"Deltam.z..Da.":0.00015,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":485380.59375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":15.70106,"Ion.Inject.Time..ms.":22,"RT..min.":11.2636,"First.Scan":10647,"Last.Scan":10647,"Master.Scan.s.":10636,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_06.raw","File.ID":"F18","Quan.Info":"","Peptides.Matched":1782,"XCorr":2.72,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":0.00004,"Percolator.SVMScore":1.649,"Precursor.Area":348406.53125,"Apex.RT..min.":11.26,"ptmRS..Binomial.Peptide.Score":272.8,"ptmRS..Isoform.Confidence.Probability":0.992,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(7): 787.42; y1+-H(3) O(4) P(6): 700.39; y2+-H(3) O(4) P(7): 394.21","ptmRS..Best.Site.Probabilities":"T11(Phospho): 99.24","ptmRS..Phospho.Site.Probabilities":"S(1): 0.0; S(2): 0.0; S(10): 0.8; T(11): 99.2"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (H4)","Search.ID":"H","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KDELTLEGIK","Annotated.Sequence":"[K].KDELTLEGIK.[Q]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.4986,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":573.32471,"Contaminant":"False","Aligned.m.z..Da.":573.32469,"MH...Da.":1145.64215,"Theo..MH...Da.":1145.64123,"DeltaM..ppm.":0.8,"Deltam.z..Da.":0.00046,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1517013.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":8.07844,"Ion.Inject.Time..ms.":22,"RT..min.":10.3199,"First.Scan":9402,"Last.Scan":9402,"Master.Scan.s.":9397,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_05.raw","File.ID":"F29","Quan.Info":"","Peptides.Matched":301,"XCorr":3.69,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":6.031e-07,"Percolator.SVMScore":2.817,"Precursor.Area":4007308.5,"Apex.RT..min.":10.34,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (H4)","Search.ID":"H","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KDELTLEGIK","Annotated.Sequence":"[K].KDELTLEGIK.[Q]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.5724,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":382.55189,"Contaminant":"False","Aligned.m.z..Da.":382.55187,"MH...Da.":1145.64112,"Theo..MH...Da.":1145.64123,"DeltaM..ppm.":-0.09,"Deltam.z..Da.":-0.00004,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2024767.625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":42.02265,"Ion.Inject.Time..ms.":22,"RT..min.":10.3295,"First.Scan":9415,"Last.Scan":9415,"Master.Scan.s.":9410,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_05.raw","File.ID":"F29","Quan.Info":"","Peptides.Matched":307,"XCorr":3.04,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":0.00007,"Percolator.SVMScore":1.741,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (H4)","Search.ID":"H","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"AAEAGETGAATSATEGDNNNNTAAGDKK","Annotated.Sequence":"[K].AAEAGETGAATsATEGDNNNNTAAGDKK.[G]","Modifications":"S12(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0434,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":906.04997,"Contaminant":"False","Aligned.m.z..Da.":906.04992,"MH...Da.":2716.13535,"Theo..MH...Da.":2716.13298,"DeltaM..ppm.":0.87,"Deltam.z..Da.":0.00079,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":6609851,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":1.44918,"Ion.Inject.Time..ms.":22,"RT..min.":5.0171,"First.Scan":1924,"Last.Scan":1924,"Master.Scan.s.":1922,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_05.raw","File.ID":"F29","Quan.Info":"","Peptides.Matched":2519,"XCorr":7.15,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":4.021e-13,"Percolator.SVMScore":6.011,"Precursor.Area":4758500.5,"Apex.RT..min.":5.02,"ptmRS..Binomial.Peptide.Score":499.14,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(18): 1789.79; y1+-H(3) O(4) P(17): 1688.75; y2+-H(3) O(4) P(25): 1174.02; y2+-H(3) O(4) P(22): 1045.47; y2+-H(3) O(4) P(18): 895.40; y2+-H(3) O(4) P(17): 844.88","ptmRS..Best.Site.Probabilities":"S12(Phospho): 99.99","ptmRS..Phospho.Site.Probabilities":"T(7): 0.0; T(11): 0.0; S(12): 100.0; T(14): 0.0; T(22): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (H4)","Search.ID":"H","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LQETNPEEVPK","Annotated.Sequence":"[K].LQETNPEEVPK.[F]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":3,"DeltaScore":0.4901,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":642.32767,"Contaminant":"False","Aligned.m.z..Da.":642.32779,"MH...Da.":1283.64805,"Theo..MH...Da.":1283.64777,"DeltaM..ppm.":0.22,"Deltam.z..Da.":0.00014,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":10236838,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":28.12667,"Ion.Inject.Time..ms.":14.439,"RT..min.":7.6424,"First.Scan":5869,"Last.Scan":5869,"Master.Scan.s.":5861,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_05.raw","File.ID":"F29","Quan.Info":"","Peptides.Matched":527,"XCorr":2.53,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":0.00008,"Percolator.SVMScore":1.721,"Precursor.Area":10126521,"Apex.RT..min.":7.65,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (H4)","Search.ID":"H","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"IQDPQMTGYVSTR","Annotated.Sequence":"[R].IQDPQmtGYVsTR.[Y]","Modifications":"M6(Oxidation); T7(Phospho); S11(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P32485","Master.Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","Protein.Accessions":"P32485","Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.0387,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":836.32721,"Contaminant":"False","Aligned.m.z..Da.":836.32737,"MH...Da.":1671.64714,"Theo..MH...Da.":1671.64853,"DeltaM..ppm.":-0.83,"Deltam.z..Da.":-0.0007,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3598734.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":2.50611,"Ion.Inject.Time..ms.":22,"RT..min.":7.6398,"First.Scan":5865,"Last.Scan":5865,"Master.Scan.s.":5861,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_05.raw","File.ID":"F29","Quan.Info":"","Peptides.Matched":743,"XCorr":3.36,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":0.00007,"Percolator.SVMScore":1.746,"Precursor.Area":3355958,"Apex.RT..min.":7.65,"ptmRS..Binomial.Peptide.Score":348.96,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(11): 1332.53; y1+-H(3) O(4) P(10): 1217.50; y1+-H(3) O(4) P(8): 992.39; y1+-H(3) O(4) P(7): 845.36; y2+-H(3) O(4) P(11): 666.77; y2+-H(3) O(4) P(10): 609.25; y1+(4): 462.27; y1+(3): 363.20; y1+(2): 276.17","ptmRS..Best.Site.Probabilities":"T7(Phospho): 100; Y9(Phospho): 99.61","ptmRS..Phospho.Site.Probabilities":"T(7): 100.0; Y(9): 99.6; S(11): 0.0; T(12): 0.4"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (H4)","Search.ID":"H","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"IGNNELLHR","Annotated.Sequence":"[K].IGNNELLHR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q03558","Master.Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","Protein.Accessions":"Q03558","Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.588,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":355.86465,"Contaminant":"False","Aligned.m.z..Da.":355.86472,"MH...Da.":1065.57941,"Theo..MH...Da.":1065.57997,"DeltaM..ppm.":-0.52,"Deltam.z..Da.":-0.00019,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":4011115,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":8.66993,"Ion.Inject.Time..ms.":22,"RT..min.":7.6608,"First.Scan":5891,"Last.Scan":5891,"Master.Scan.s.":5887,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_05.raw","File.ID":"F29","Quan.Info":"","Peptides.Matched":300,"XCorr":2.5,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":0.00004,"Percolator.SVMScore":1.886,"Precursor.Area":2057189.125,"Apex.RT..min.":7.66,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (H4)","Search.ID":"H","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LQETNPEEVPK","Annotated.Sequence":"[K].LQETNPEEVPK.[F]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.536,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":642.32767,"Contaminant":"False","Aligned.m.z..Da.":642.32779,"MH...Da.":1283.64806,"Theo..MH...Da.":1283.64777,"DeltaM..ppm.":0.23,"Deltam.z..Da.":0.00015,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":5385115,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":22.5687,"Ion.Inject.Time..ms.":22,"RT..min.":7.6288,"First.Scan":5851,"Last.Scan":5851,"Master.Scan.s.":5848,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_05.raw","File.ID":"F29","Quan.Info":"","Peptides.Matched":527,"XCorr":2.5,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":0.00018,"Percolator.SVMScore":1.518,"Precursor.Area":10126521,"Apex.RT..min.":7.65,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (H4)","Search.ID":"H","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"IQDPQMTGYVSTR","Annotated.Sequence":"[R].IQDPQmTGyVSTR.[Y]","Modifications":"M6(Oxidation); Y9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P32485","Master.Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","Protein.Accessions":"P32485","Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1297,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":796.34435,"Contaminant":"False","Aligned.m.z..Da.":796.34449,"MH...Da.":1591.68142,"Theo..MH...Da.":1591.6822,"DeltaM..ppm.":-0.49,"Deltam.z..Da.":-0.00039,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3902704.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":7.84759,"Ion.Inject.Time..ms.":22,"RT..min.":7.5683,"First.Scan":5777,"Last.Scan":5777,"Master.Scan.s.":5770,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_05.raw","File.ID":"F29","Quan.Info":"","Peptides.Matched":1285,"XCorr":3.16,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":3.148e-06,"Percolator.SVMScore":2.446,"Precursor.Area":8877059,"Apex.RT..min.":7.6,"ptmRS..Binomial.Peptide.Score":276.29,"ptmRS..Isoform.Confidence.Probability":0.992,"ptmRS..Isoform.Group.Report":"y1+(6): 762.32; y1+(5): 705.30","ptmRS..Best.Site.Probabilities":"Y9(Phospho): 99.17","ptmRS..Phospho.Site.Probabilities":"T(7): 0.4; Y(9): 99.2; S(11): 0.4; T(12): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (H4)","Search.ID":"H","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSSPLHRLSPTGK","Annotated.Sequence":"[R].mSsPLHRLsPTGK.[S]","Modifications":"M1(Oxidation); S3(Phospho); S9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5263,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":793.84286,"Contaminant":"False","Aligned.m.z..Da.":793.84303,"MH...Da.":1586.67845,"Theo..MH...Da.":1586.67977,"DeltaM..ppm.":-0.83,"Deltam.z..Da.":-0.00066,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2503364,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":17.31418,"Ion.Inject.Time..ms.":22,"RT..min.":7.7166,"First.Scan":5962,"Last.Scan":5962,"Master.Scan.s.":5952,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_05.raw","File.ID":"F29","Quan.Info":"","Peptides.Matched":965,"XCorr":2.66,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":0.00009,"Percolator.SVMScore":1.695,"Precursor.Area":3408272,"Apex.RT..min.":7.73,"ptmRS..Binomial.Peptide.Score":273.75,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(3): 304.10; y2+-H(3) O(4) P(11): 627.82; y2+- -2H(3) O(4) P -2H(3) O(4) P(11): 578.83; b1+(2): 235.07","ptmRS..Best.Site.Probabilities":"S3(Phospho): 100; S9(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(3): 100.0; S(9): 100.0; T(11): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (H4)","Search.ID":"H","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSSPLHRLSPTGK","Annotated.Sequence":"[R].mSsPLHRLsPTGK.[S]","Modifications":"M1(Oxidation); S3(Phospho); S9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.4729,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":529.56486,"Contaminant":"False","Aligned.m.z..Da.":529.56497,"MH...Da.":1586.68003,"Theo..MH...Da.":1586.67977,"DeltaM..ppm.":0.16,"Deltam.z..Da.":0.00009,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":4011854.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":7.02314,"Ion.Inject.Time..ms.":22,"RT..min.":7.7049,"First.Scan":5947,"Last.Scan":5947,"Master.Scan.s.":5939,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_05.raw","File.ID":"F29","Quan.Info":"","Peptides.Matched":977,"XCorr":4.42,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":7.362e-06,"Percolator.SVMScore":2.255,"Precursor.Area":8291505,"Apex.RT..min.":7.73,"ptmRS..Binomial.Peptide.Score":296.6,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(9): 990.58; y1+-H(3) O(4) P(8): 877.50; y1+-H(3) O(4) P(5): 471.26; y2+-H(3) O(4) P(11): 627.82; y2+-H(3) O(4) P(10): 544.32; y2+-H(3) O(4) P(8): 439.25; y1+(4): 402.23; y1+(3): 305.18","ptmRS..Best.Site.Probabilities":"S3(Phospho): 100; S9(Phospho): 99.99","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(3): 100.0; S(9): 100.0; T(11): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (H4)","Search.ID":"H","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"AAEAGETGAATSATEGDNNNNTAAGDKK","Annotated.Sequence":"[K].AAEAGETGAATSATEGDNNNNTAAGDKK.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.7531,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":879.39419,"Contaminant":"False","Aligned.m.z..Da.":879.39413,"MH...Da.":2636.16803,"Theo..MH...Da.":2636.16665,"DeltaM..ppm.":0.53,"Deltam.z..Da.":0.00046,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2797656.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":4.9472,"First.Scan":1843,"Last.Scan":1843,"Master.Scan.s.":1831,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_05.raw","File.ID":"F29","Quan.Info":"","Peptides.Matched":3537,"XCorr":8.14,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":1e-17,"Percolator.SVMScore":8.392,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (H4)","Search.ID":"H","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"HSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[R].HSDSYsENETNHTNVPISSTGGTNNK.[T]","Modifications":"S6(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":0,"Charge":4,"Original.Precursor.Charge":2,"DeltaScore":0.0029,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":718.30175,"Contaminant":"False","Aligned.m.z..Da.":718.30188,"MH...Da.":2870.18519,"Theo..MH...Da.":2870.18608,"DeltaM..ppm.":-0.31,"Deltam.z..Da.":-0.00022,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":5640162.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":35.25486,"Ion.Inject.Time..ms.":10.265,"RT..min.":7.5244,"First.Scan":5722,"Last.Scan":5722,"Master.Scan.s.":5718,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_05.raw","File.ID":"F29","Quan.Info":"","Peptides.Matched":2489,"XCorr":3.41,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":1.507e-06,"Percolator.SVMScore":2.611,"Precursor.Area":6139054,"Apex.RT..min.":7.51,"ptmRS..Binomial.Peptide.Score":235.64,"ptmRS..Isoform.Confidence.Probability":0.493,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(11): 1246.46; b2+-H(3) O(4) P(14): 799.81; b2+-H(3) O(4) P(15): 849.34","ptmRS..Best.Site.Probabilities":"S6(Phospho): 49.32; T10(Phospho): 49.32","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(4): 0.7; Y(5): 0.0; S(6): 49.3; T(10): 49.3; T(13): 0.7; S(18): 0.0; S(19): 0.0; T(20): 0.0; T(23): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (H4)","Search.ID":"H","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"HSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[R].HSDSySENETNHTNVPISSTGGTNNK.[T]","Modifications":"Y5(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":0,"Charge":4,"Original.Precursor.Charge":4,"DeltaScore":0.0054,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":718.30298,"Contaminant":"False","Aligned.m.z..Da.":718.30311,"MH...Da.":2870.19011,"Theo..MH...Da.":2870.18608,"DeltaM..ppm.":1.41,"Deltam.z..Da.":0.00101,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1667087.125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":7.4944,"First.Scan":5685,"Last.Scan":5685,"Master.Scan.s.":5679,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_05.raw","File.ID":"F29","Quan.Info":"","Peptides.Matched":2535,"XCorr":5.54,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":2.255e-08,"Percolator.SVMScore":3.555,"Precursor.Area":6139054,"Apex.RT..min.":7.51,"ptmRS..Binomial.Peptide.Score":291.53,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(9): 1031.37; b1+-H(3) O(4) P(11): 1246.46; b1+-H(3) O(4) P(15): 1697.68; b2+-H(3) O(4) P(7): 394.65; b2+-H(3) O(4) P(8): 451.67; b2+-H(3) O(4) P(14): 799.81; b2+-H(3) O(4) P(15): 849.34; b2+-H(3) O(4) P(17): 954.41; b1+(4): 427.16","ptmRS..Best.Site.Probabilities":"S6(Phospho): 99.38","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(4): 0.6; Y(5): 0.0; S(6): 99.4; T(10): 0.0; T(13): 0.0; S(18): 0.0; S(19): 0.0; T(20): 0.0; T(23): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (H4)","Search.ID":"H","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"HSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[R].HSDSySENETNHTNVPISSTGGTNNK.[T]","Modifications":"Y5(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0397,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":957.40032,"Contaminant":"False","Aligned.m.z..Da.":957.40048,"MH...Da.":2870.1864,"Theo..MH...Da.":2870.18608,"DeltaM..ppm.":0.11,"Deltam.z..Da.":0.00011,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3291252,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":7.4931,"First.Scan":5683,"Last.Scan":5683,"Master.Scan.s.":5679,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_05.raw","File.ID":"F29","Quan.Info":"","Peptides.Matched":2512,"XCorr":7.3,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":1.625e-13,"Percolator.SVMScore":6.214,"Precursor.Area":12540091,"Apex.RT..min.":7.51,"ptmRS..Binomial.Peptide.Score":308.66,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(6): 659.24; b1+-H(3) O(4) P(9): 1031.37; b1+-H(3) O(4) P(11): 1246.46; b1+-H(3) O(4) P(12): 1383.52; b1+-H(3) O(4) P(15): 1697.68; b2+-H(3) O(4) P(12): 692.26; b2+-H(3) O(4) P(13): 742.79; b2+-H(3) O(4) P(14): 799.81; b2+-H(3) O(4) P(15): 849.34; y2+-H(3) O(4) P(25): 1318.08; y2+-H(3) O(4) P(24): 1274.56; y2+-H(3) O(4) P(21): 1092.00; b1+(4): 427.16","ptmRS..Best.Site.Probabilities":"S6(Phospho): 99.36","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(4): 0.6; Y(5): 0.0; S(6): 99.4; T(10): 0.0; T(13): 0.0; S(18): 0.0; S(19): 0.0; T(20): 0.0; T(23): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (H4)","Search.ID":"H","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MGSINNNFNTNNNSNTDLDRDWK","Annotated.Sequence":"[-].mGSINNNFNTNNNsNTDLDRDWK.[T]","Modifications":"N-Term(Prot)(Met-loss); S14(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P39517","Master.Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","Protein.Accessions":"P39517","Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0642,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":878.3724,"Contaminant":"False","Aligned.m.z..Da.":878.37226,"MH...Da.":2633.10264,"Theo..MH...Da.":2633.10123,"DeltaM..ppm.":0.54,"Deltam.z..Da.":0.00047,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1124103.625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":11.243,"First.Scan":10661,"Last.Scan":10661,"Master.Scan.s.":10658,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_05.raw","File.ID":"F29","Quan.Info":"","Peptides.Matched":2285,"XCorr":5.14,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":1.222e-09,"Percolator.SVMScore":4.21,"Precursor.Area":2177756.5,"Apex.RT..min.":11.26,"ptmRS..Binomial.Peptide.Score":496.36,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(13): 1573.70; y1+-H(3) O(4) P(12): 1459.66; y1+-H(3) O(4) P(11): 1345.61; y1+-H(3) O(4) P(10): 1231.57; y2+-H(3) O(4) P(19): 1139.50; y2+-H(3) O(4) P(17): 1025.45; y2+-H(3) O(4) P(16): 968.43; y2+-H(3) O(4) P(15): 894.90; y2+-H(3) O(4) P(14): 837.88; y2+-H(3) O(4) P(13): 787.35; y2+-H(3) O(4) P(12): 730.33; y2+-H(3) O(4) P(11): 673.31; y2+-H(3) O(4) P(10): 616.29; y1+(9): 1162.55; y1+(8): 1048.51","ptmRS..Best.Site.Probabilities":"S14(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(3): 0.0; T(10): 0.0; S(14): 100.0; T(16): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (H4)","Search.ID":"H","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"SSQIHIENESTEDILK","Annotated.Sequence":"[R].SSQIHIENEStEDILK.[I]","Modifications":"T11(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0039,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":641.62908,"Contaminant":"False","Aligned.m.z..Da.":641.62898,"MH...Da.":1922.8727,"Theo..MH...Da.":1922.87429,"DeltaM..ppm.":-0.83,"Deltam.z..Da.":-0.00053,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1039871.6875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":29.40701,"Ion.Inject.Time..ms.":22,"RT..min.":11.2758,"First.Scan":10708,"Last.Scan":10708,"Master.Scan.s.":10697,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_05.raw","File.ID":"F29","Quan.Info":"","Peptides.Matched":1830,"XCorr":2.58,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":0.00012,"Percolator.SVMScore":1.611,"Precursor.Area":841533.0625,"Apex.RT..min.":11.28,"ptmRS..Binomial.Peptide.Score":249,"ptmRS..Isoform.Confidence.Probability":0.991,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(7): 787.42; y1+-H(3) O(4) P(6): 700.39; y2+-H(3) O(4) P(7): 394.21","ptmRS..Best.Site.Probabilities":"T11(Phospho): 99.14","ptmRS..Phospho.Site.Probabilities":"S(1): 0.0; S(2): 0.0; S(10): 0.9; T(11): 99.1"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (H4)","Search.ID":"H","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KSDEPSRESTPVR","Annotated.Sequence":"[K].KSDEPsREsTPVR.[S]","Modifications":"S6(Phospho); S9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q08421","Master.Protein.Descriptions":"Enhancer of translation termination 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ETT1 PE=1 SV=1","Protein.Accessions":"Q08421","Protein.Descriptions":"Enhancer of translation termination 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ETT1 PE=1 SV=1","X..Missed.Cleavages":2,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.0874,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":824.3426,"Contaminant":"False","Aligned.m.z..Da.":824.34243,"MH...Da.":1647.67793,"Theo..MH...Da.":1647.67752,"DeltaM..ppm.":0.25,"Deltam.z..Da.":0.0002,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":9735062,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":5.05322,"Ion.Inject.Time..ms.":12.93,"RT..min.":4.5208,"First.Scan":1356,"Last.Scan":1356,"Master.Scan.s.":1350,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_05.raw","File.ID":"F29","Quan.Info":"","Peptides.Matched":968,"XCorr":2.86,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":0.00012,"Percolator.SVMScore":1.608,"Precursor.Area":10487327,"Apex.RT..min.":4.53,"ptmRS..Binomial.Peptide.Score":348.61,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(9): 998.45; y1+-H(3) O(4) P(10): 1219.55; y1+-H(3) O(4) P(9): 1090.50; y1+-H(3) O(4) P(4): 454.28; y2+-H(3) O(4) P(10): 610.28; y2+-H(3) O(4) P(9): 545.76; b1+- -2H(3) O(4) P -2H(3) O(4) P(10): 1081.49","ptmRS..Best.Site.Probabilities":"S6(Phospho): 100; T10(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(6): 100.0; S(9): 0.0; T(10): 100.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (H4)","Search.ID":"H","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LQETNPEEVPKFEK","Annotated.Sequence":"[K].LQETNPEEVPKFEK.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.5235,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":563.29054,"Contaminant":"False","Aligned.m.z..Da.":563.29057,"MH...Da.":1687.85709,"Theo..MH...Da.":1687.85374,"DeltaM..ppm.":1.98,"Deltam.z..Da.":0.00112,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2597454.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":46.48624,"Ion.Inject.Time..ms.":22,"RT..min.":9.3106,"First.Scan":8042,"Last.Scan":8042,"Master.Scan.s.":8032,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_05.raw","File.ID":"F29","Quan.Info":"","Peptides.Matched":847,"XCorr":1.49,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":0.00007,"Percolator.SVMScore":1.74,"Precursor.Area":2400221,"Apex.RT..min.":9.32,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (H4)","Search.ID":"H","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"AAEAGETGAATSATEGDNNNNTAAGDK","Annotated.Sequence":"[K].AAEAGETGAATSATEGDNNNNTAAGDK.[K]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.6864,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":836.69699,"Contaminant":"False","Aligned.m.z..Da.":836.69701,"MH...Da.":2508.07643,"Theo..MH...Da.":2508.07169,"DeltaM..ppm.":1.89,"Deltam.z..Da.":0.00158,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":957184.8125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":5.613,"First.Scan":2742,"Last.Scan":2742,"Master.Scan.s.":2741,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_05.raw","File.ID":"F29","Quan.Info":"","Peptides.Matched":2789,"XCorr":4.72,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":1.337e-08,"Percolator.SVMScore":3.672,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (H4)","Search.ID":"H","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"AGNFALHPEVVR","Annotated.Sequence":"[R].AGNFALHPEVVR.[E]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q03558","Master.Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","Protein.Accessions":"Q03558","Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.7336,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":437.23817,"Contaminant":"False","Aligned.m.z..Da.":437.23816,"MH...Da.":1309.69997,"Theo..MH...Da.":1309.70114,"DeltaM..ppm.":-0.9,"Deltam.z..Da.":-0.00039,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2494191,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":6.23345,"Ion.Inject.Time..ms.":22,"RT..min.":10.086,"First.Scan":9087,"Last.Scan":9087,"Master.Scan.s.":9085,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_05.raw","File.ID":"F29","Quan.Info":"","Peptides.Matched":382,"XCorr":3.04,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":8.82e-07,"Percolator.SVMScore":2.732,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (H4)","Search.ID":"H","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"HSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[R].HSDsYsENETNHTNVPISSTGGTNNK.[T]","Modifications":"S4(Phospho); S6(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.1196,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":984.05587,"Contaminant":"False","Aligned.m.z..Da.":984.0561,"MH...Da.":2950.15307,"Theo..MH...Da.":2950.15241,"DeltaM..ppm.":0.23,"Deltam.z..Da.":0.00022,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":4155173.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":7.9383,"First.Scan":6241,"Last.Scan":6241,"Master.Scan.s.":6238,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_05.raw","File.ID":"F29","Quan.Info":"","Peptides.Matched":1121,"XCorr":6.69,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":8.98e-10,"Percolator.SVMScore":4.279,"Precursor.Area":5818695,"Apex.RT..min.":7.95,"ptmRS..Binomial.Peptide.Score":609.53,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(4): 409.15; b1+-H(3) O(4) P(5): 572.21; b1+-H(3) O(4) P(6): 739.21; b1+-H(3) O(4) P(11): 1326.43; b1+-H(3) O(4) P(15): 1777.64; b2+-H(3) O(4) P(12): 732.25; b2+-H(3) O(4) P(14): 839.79; b2+-H(3) O(4) P(15): 889.33; b1+(2): 225.10; b1+(3): 340.13","ptmRS..Best.Site.Probabilities":"S4(Phospho): 99.99; S6(Phospho): 99.99","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(4): 100.0; Y(5): 0.0; S(6): 100.0; T(10): 0.0; T(13): 0.0; S(18): 0.0; S(19): 0.0; T(20): 0.0; T(23): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (H4)","Search.ID":"H","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"APQALMLAPTR","Annotated.Sequence":"[K].APQALmLAPTR.[E]","Modifications":"M6(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6734,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":592.8269,"Contaminant":"False","Aligned.m.z..Da.":592.82688,"MH...Da.":1184.64653,"Theo..MH...Da.":1184.6456,"DeltaM..ppm.":0.78,"Deltam.z..Da.":0.00046,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1313301.625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":20.98831,"Ion.Inject.Time..ms.":22,"RT..min.":10.217,"First.Scan":9265,"Last.Scan":9265,"Master.Scan.s.":9254,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_05.raw","File.ID":"F29","Quan.Info":"","Peptides.Matched":384,"XCorr":2.97,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":0.00009,"Percolator.SVMScore":1.683,"Precursor.Area":3598662,"Apex.RT..min.":10.24,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (H4)","Search.ID":"H","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"DFRHSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[K].DFRHSDsYsENETNHTNVPISSTGGTNNK.[T]","Modifications":"S7(Phospho); S9(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":1,"Charge":4,"Original.Precursor.Charge":4,"DeltaScore":0.035,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":842.84192,"Contaminant":"False","Aligned.m.z..Da.":842.84209,"MH...Da.":3368.34588,"Theo..MH...Da.":3368.34888,"DeltaM..ppm.":-0.89,"Deltam.z..Da.":-0.00075,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2432199.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":8.5749,"First.Scan":7060,"Last.Scan":7060,"Master.Scan.s.":7057,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_05.raw","File.ID":"F29","Quan.Info":"","Peptides.Matched":912,"XCorr":6.57,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":2.7e-08,"Percolator.SVMScore":3.515,"Precursor.Area":2776538.75,"Apex.RT..min.":8.57,"ptmRS..Binomial.Peptide.Score":433.92,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"b2+-H(3) O(4) P(7): 454.16; b2+-H(3) O(4) P(8): 535.69; b1+- -2H(3) O(4) P -2H(3) O(4) P(12): 1431.56; b2+- -2H(3) O(4) P -2H(3) O(4) P(15): 892.36; b2+- -2H(3) O(4) P -2H(3) O(4) P(17): 999.90; b2+- -2H(3) O(4) P -2H(3) O(4) P(18): 1049.44; b2+- -2H(3) O(4) P -2H(3) O(4) P(20): 1154.50","ptmRS..Best.Site.Probabilities":"S5(Phospho): 100; S7(Phospho): 98.8","ptmRS..Phospho.Site.Probabilities":"S(5): 100.0; S(7): 98.8; Y(8): 0.0; S(9): 1.2; T(13): 0.0; T(16): 0.0; S(21): 0.0; S(22): 0.0; T(23): 0.0; T(26): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (H4)","Search.ID":"H","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"NQQLEDEIEK","Annotated.Sequence":"[K].NQQLEDEIEK.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5623,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":623.30088,"Contaminant":"False","Aligned.m.z..Da.":623.301,"MH...Da.":1245.59449,"Theo..MH...Da.":1245.59574,"DeltaM..ppm.":-1,"Deltam.z..Da.":-0.00062,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3129625.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":25.5476,"Ion.Inject.Time..ms.":22,"RT..min.":8.6093,"First.Scan":7107,"Last.Scan":7107,"Master.Scan.s.":7096,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_05.raw","File.ID":"F29","Quan.Info":"","Peptides.Matched":648,"XCorr":2.65,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":0.00003,"Percolator.SVMScore":1.953,"Precursor.Area":1848199.125,"Apex.RT..min.":8.6,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (H4)","Search.ID":"H","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"AAEAGETGAATSATEGDNNNNTAAGDK","Annotated.Sequence":"[K].AAEAGETGAATsATEGDNNNNTAAGDK.[K]","Modifications":"S12(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0216,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":863.35003,"Contaminant":"False","Aligned.m.z..Da.":863.35005,"MH...Da.":2588.03554,"Theo..MH...Da.":2588.03802,"DeltaM..ppm.":-0.96,"Deltam.z..Da.":-0.00083,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2039462.375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":3.66813,"Ion.Inject.Time..ms.":22,"RT..min.":5.7244,"First.Scan":2924,"Last.Scan":2924,"Master.Scan.s.":2923,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_05.raw","File.ID":"F29","Quan.Info":"","Peptides.Matched":1457,"XCorr":6.02,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":5.05e-09,"Percolator.SVMScore":3.891,"Precursor.Area":1519842,"Apex.RT..min.":5.73,"ptmRS..Binomial.Peptide.Score":428.02,"ptmRS..Isoform.Confidence.Probability":0.497,"ptmRS..Isoform.Group.Report":"b2+-H(3) O(4) P(26): 1172.48; y2+-H(3) O(4) P(25): 1174.50; y2+-H(3) O(4) P(21): 981.43","ptmRS..Best.Site.Probabilities":"T11(Phospho): 49.69; S12(Phospho): 49.69","ptmRS..Phospho.Site.Probabilities":"T(7): 0.3; T(11): 49.7; S(12): 49.7; T(14): 0.3; T(22): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (H4)","Search.ID":"H","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ILISTDLLAR","Annotated.Sequence":"[R].ILISTDLLAR.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.4796,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":557.8456,"Contaminant":"False","Aligned.m.z..Da.":557.84542,"MH...Da.":1114.68393,"Theo..MH...Da.":1114.68304,"DeltaM..ppm.":0.81,"Deltam.z..Da.":0.00045,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":465088.21875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":14.07059,"Ion.Inject.Time..ms.":22,"RT..min.":15.7392,"First.Scan":16468,"Last.Scan":16468,"Master.Scan.s.":16467,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_05.raw","File.ID":"F29","Quan.Info":"","Peptides.Matched":124,"XCorr":2.21,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":0.00015,"Percolator.SVMScore":1.562,"Precursor.Area":2313416,"Apex.RT..min.":15.7,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (H4)","Search.ID":"H","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0389,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40304,"Contaminant":"False","Aligned.m.z..Da.":967.40272,"MH...Da.":2900.19457,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":-1.64,"Deltam.z..Da.":-0.00159,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":330791.3125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":20.16457,"Ion.Inject.Time..ms.":22,"RT..min.":15.7202,"First.Scan":16444,"Last.Scan":16444,"Master.Scan.s.":16442,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_05.raw","File.ID":"F29","Quan.Info":"","Peptides.Matched":2262,"XCorr":4.37,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":2.767e-07,"Percolator.SVMScore":2.992,"Precursor.Area":547002,"Apex.RT..min.":15.81,"ptmRS..Binomial.Peptide.Score":332.19,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y2+-H(3) O(4) P(14): 811.37; b1+(12): 1344.56","ptmRS..Best.Site.Probabilities":"S13(Phospho): 99.99","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (H4)","Search.ID":"H","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].mSEGItDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); T6(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.1506,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":731.31368,"Contaminant":"False","Aligned.m.z..Da.":731.31354,"MH...Da.":2191.92649,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":-0.62,"Deltam.z..Da.":-0.00045,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":377780.1875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":44.01771,"Ion.Inject.Time..ms.":22,"RT..min.":15.3407,"First.Scan":15963,"Last.Scan":15963,"Master.Scan.s.":15955,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_05.raw","File.ID":"F29","Quan.Info":"","Peptides.Matched":1958,"XCorr":2.39,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":0.00014,"Percolator.SVMScore":1.576,"Precursor.Area":315994.65625,"Apex.RT..min.":15.32,"ptmRS..Binomial.Peptide.Score":208.4,"ptmRS..Isoform.Confidence.Probability":0.009,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(2): 112.04; b1+-H(3) O(4) P(3): 241.08; b1+-H(3) O(4) P(4): 298.10; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35","ptmRS..Best.Site.Probabilities":"S2(Phospho): 99.06","ptmRS..Phospho.Site.Probabilities":"S(2): 99.1; T(6): 0.9; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (H4)","Search.ID":"H","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"GVFGYGFEEPSAIQQR","Annotated.Sequence":"[R].GVFGYGFEEPSAIQQR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6641,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":892.93412,"Contaminant":"False","Aligned.m.z..Da.":892.93405,"MH...Da.":1784.86097,"Theo..MH...Da.":1784.86022,"DeltaM..ppm.":0.42,"Deltam.z..Da.":0.00037,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":687362.3125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":15.0516,"First.Scan":15578,"Last.Scan":15578,"Master.Scan.s.":15577,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_05.raw","File.ID":"F29","Quan.Info":"","Peptides.Matched":1404,"XCorr":3.93,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":9.803e-09,"Percolator.SVMScore":3.742,"Precursor.Area":1015693.3125,"Apex.RT..min.":15.07,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (H4)","Search.ID":"H","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FTVSAIYSDLPQQER","Annotated.Sequence":"[K].FTVSAIYSDLPQQER.[D]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6991,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":877.4411,"Contaminant":"False","Aligned.m.z..Da.":877.44105,"MH...Da.":1753.87493,"Theo..MH...Da.":1753.87554,"DeltaM..ppm.":-0.35,"Deltam.z..Da.":-0.00031,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":697826.3125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":8.73838,"Ion.Inject.Time..ms.":22,"RT..min.":14.9847,"First.Scan":15490,"Last.Scan":15490,"Master.Scan.s.":15486,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_05.raw","File.ID":"F29","Quan.Info":"","Peptides.Matched":1124,"XCorr":4.22,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":4.842e-09,"Percolator.SVMScore":3.901,"Precursor.Area":565657.5625,"Apex.RT..min.":15,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (H4)","Search.ID":"H","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":5,"DeltaScore":0.0695,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40438,"Contaminant":"False","Aligned.m.z..Da.":967.40433,"MH...Da.":2900.1986,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":-0.25,"Deltam.z..Da.":-0.00024,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":14491652,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":3.17697,"Ion.Inject.Time..ms.":6.443,"RT..min.":14.9841,"First.Scan":15489,"Last.Scan":15489,"Master.Scan.s.":15486,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_05.raw","File.ID":"F29","Quan.Info":"","Peptides.Matched":2444,"XCorr":7.77,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":3.331e-14,"Percolator.SVMScore":6.57,"Precursor.Area":18717604,"Apex.RT..min.":14.97,"ptmRS..Binomial.Peptide.Score":814.6,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(13): 1413.58; b1+-H(3) O(4) P(14): 1528.61; b1+-H(3) O(4) P(15): 1642.65; b1+-H(3) O(4) P(16): 1771.69; b1+-H(3) O(4) P(18): 2028.79; y1+-H(3) O(4) P(15): 1750.78; y1+-H(3) O(4) P(14): 1621.73; y1+-H(3) O(4) P(13): 1458.67; b2+-H(3) O(4) P(16): 886.35; b2+-H(3) O(4) P(18): 1014.90; y2+-H(3) O(4) P(14): 811.37; b1+(12): 1344.56; y1+(13): 1556.65","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (H4)","Search.ID":"H","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":5,"DeltaScore":0.0691,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40402,"Contaminant":"False","Aligned.m.z..Da.":967.40397,"MH...Da.":2900.1975,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":-0.63,"Deltam.z..Da.":-0.00061,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":16103436,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":2.47977,"Ion.Inject.Time..ms.":8.102,"RT..min.":14.9785,"First.Scan":15483,"Last.Scan":15483,"Master.Scan.s.":15473,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_05.raw","File.ID":"F29","Quan.Info":"","Peptides.Matched":2398,"XCorr":7.38,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":3.357e-15,"Percolator.SVMScore":7.086,"Precursor.Area":18717604,"Apex.RT..min.":14.97,"ptmRS..Binomial.Peptide.Score":886.73,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(13): 1413.58; b1+-H(3) O(4) P(14): 1528.61; b1+-H(3) O(4) P(15): 1642.65; b1+-H(3) O(4) P(16): 1771.69; b1+-H(3) O(4) P(17): 1899.75; b1+-H(3) O(4) P(19): 2141.88; y1+-H(3) O(4) P(15): 1750.78; y1+-H(3) O(4) P(14): 1621.73; y1+-H(3) O(4) P(13): 1458.67; b2+-H(3) O(4) P(16): 886.35; b2+-H(3) O(4) P(17): 950.38; b2+-H(3) O(4) P(18): 1014.90; y2+-H(3) O(4) P(14): 811.37; b1+(12): 1344.56; y1+(13): 1556.65","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (H4)","Search.ID":"H","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"AIMPIIEGHDVLAQAQSGTGK","Annotated.Sequence":"[R].AImPIIEGHDVLAQAQSGTGK.[T]","Modifications":"M3(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.7146,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":718.03952,"Contaminant":"False","Aligned.m.z..Da.":718.03953,"MH...Da.":2152.10401,"Theo..MH...Da.":2152.10668,"DeltaM..ppm.":-1.24,"Deltam.z..Da.":-0.00089,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1166703.875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":4.62931,"Ion.Inject.Time..ms.":22,"RT..min.":12.6569,"First.Scan":12578,"Last.Scan":12578,"Master.Scan.s.":12569,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_05.raw","File.ID":"F29","Quan.Info":"","Peptides.Matched":1029,"XCorr":5.08,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":1.297e-10,"Percolator.SVMScore":4.714,"Precursor.Area":1469456.25,"Apex.RT..min.":12.67,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (H4)","Search.ID":"H","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0684,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40582,"Contaminant":"False","Aligned.m.z..Da.":967.40567,"MH...Da.":2900.2029,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":1.23,"Deltam.z..Da.":0.00119,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":795164.5625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":10.01498,"Ion.Inject.Time..ms.":22,"RT..min.":15.2311,"First.Scan":15814,"Last.Scan":15814,"Master.Scan.s.":15812,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_05.raw","File.ID":"F29","Quan.Info":"","Peptides.Matched":2533,"XCorr":5.12,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":3.853e-07,"Percolator.SVMScore":2.918,"Precursor.Area":18717604,"Apex.RT..min.":14.97,"ptmRS..Binomial.Peptide.Score":396.06,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(13): 1413.58; y1+-H(3) O(4) P(13): 1458.67; b1+(12): 1344.56","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (H4)","Search.ID":"H","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":4,"DeltaScore":0.0671,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40568,"Contaminant":"False","Aligned.m.z..Da.":967.40559,"MH...Da.":2900.20248,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":1.09,"Deltam.z..Da.":0.00105,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1768617.625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":24.91858,"Ion.Inject.Time..ms.":22,"RT..min.":15.0968,"First.Scan":15636,"Last.Scan":15636,"Master.Scan.s.":15631,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_05.raw","File.ID":"F29","Quan.Info":"","Peptides.Matched":2539,"XCorr":5.07,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":1.085e-08,"Percolator.SVMScore":3.719,"Precursor.Area":18717604,"Apex.RT..min.":14.97,"ptmRS..Binomial.Peptide.Score":326.41,"ptmRS..Isoform.Confidence.Probability":0.993,"ptmRS..Isoform.Group.Report":"b1+(12): 1344.56","ptmRS..Best.Site.Probabilities":"S13(Phospho): 99.25","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.8; S(13): 99.3; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (H4)","Search.ID":"H","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEySDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); Y12(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.3166,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40821,"Contaminant":"False","Aligned.m.z..Da.":967.40795,"MH...Da.":2900.21008,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":3.71,"Deltam.z..Da.":0.00358,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":315577.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":16.9639,"First.Scan":17745,"Last.Scan":17745,"Master.Scan.s.":17736,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_05.raw","File.ID":"F29","Quan.Info":"","Peptides.Matched":2668,"XCorr":1.99,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":0.00023,"Percolator.SVMScore":1.464,"ptmRS..Binomial.Peptide.Score":195.28,"ptmRS..Isoform.Confidence.Probability":0.5,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"Y12(Phospho): 50; S13(Phospho): 50","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 50.0; S(13): 50.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (H4)","Search.ID":"H","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MFILDEADEMLSSGFK","Annotated.Sequence":"[K].mFILDEADEmLSSGFK.[E]","Modifications":"M1(Oxidation); M10(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7157,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":932.92367,"Contaminant":"False","Aligned.m.z..Da.":932.92334,"MH...Da.":1864.84007,"Theo..MH...Da.":1864.83433,"DeltaM..ppm.":3.08,"Deltam.z..Da.":0.00287,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":212841.57812,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":23.39945,"Ion.Inject.Time..ms.":22,"RT..min.":16.7006,"First.Scan":17521,"Last.Scan":17521,"Master.Scan.s.":17517,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_05.raw","File.ID":"F29","Quan.Info":"","Peptides.Matched":1856,"XCorr":3.06,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":5.652e-08,"Percolator.SVMScore":3.349,"Precursor.Area":635972.9375,"Apex.RT..min.":16.82,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (H4)","Search.ID":"H","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.3462,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40596,"Contaminant":"False","Aligned.m.z..Da.":967.40556,"MH...Da.":2900.20333,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":1.38,"Deltam.z..Da.":0.00133,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":212537.42188,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":16.2518,"First.Scan":17097,"Last.Scan":17097,"Master.Scan.s.":17092,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_05.raw","File.ID":"F29","Quan.Info":"","Peptides.Matched":2586,"XCorr":4.16,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":1.107e-06,"Percolator.SVMScore":2.681,"Precursor.Area":679853.125,"Apex.RT..min.":16.57,"ptmRS..Binomial.Peptide.Score":330.64,"ptmRS..Isoform.Confidence.Probability":0.991,"ptmRS..Isoform.Group.Report":"y2+-H(3) O(4) P(14): 811.37","ptmRS..Best.Site.Probabilities":"S13(Phospho): 99.14","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.9; S(13): 99.1; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (H4)","Search.ID":"H","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FDDMELDENLLR","Annotated.Sequence":"[K].FDDMELDENLLR.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5527,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":755.34995,"Contaminant":"False","Aligned.m.z..Da.":755.34965,"MH...Da.":1509.69263,"Theo..MH...Da.":1509.68898,"DeltaM..ppm.":2.42,"Deltam.z..Da.":0.00182,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":192936.07812,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":27.80733,"Ion.Inject.Time..ms.":22,"RT..min.":16.0583,"First.Scan":16871,"Last.Scan":16871,"Master.Scan.s.":16860,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_05.raw","File.ID":"F29","Quan.Info":"","Peptides.Matched":1081,"XCorr":2.75,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":0.00001,"Percolator.SVMScore":2.134,"Precursor.Area":233380.03125,"Apex.RT..min.":16.06,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (H4)","Search.ID":"H","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DFLLKPELSR","Annotated.Sequence":"[K].DFLLKPELSR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.624,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":406.56736,"Contaminant":"False","Aligned.m.z..Da.":406.56742,"MH...Da.":1217.68755,"Theo..MH...Da.":1217.68885,"DeltaM..ppm.":-1.07,"Deltam.z..Da.":-0.00043,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":627794.875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":25.11391,"Ion.Inject.Time..ms.":22,"RT..min.":13.2186,"First.Scan":13322,"Last.Scan":13322,"Master.Scan.s.":13310,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_05.raw","File.ID":"F29","Quan.Info":"","Peptides.Matched":285,"XCorr":2.5,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":0.00012,"Percolator.SVMScore":1.61,"Precursor.Area":1069751.625,"Apex.RT..min.":13.23,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (H4)","Search.ID":"H","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"TGTFSIAALQR","Annotated.Sequence":"[K].TGTFSIAALQR.[I]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":3,"DeltaScore":0.7304,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":582.82282,"Contaminant":"False","Aligned.m.z..Da.":582.82292,"MH...Da.":1164.63837,"Theo..MH...Da.":1164.63715,"DeltaM..ppm.":1.05,"Deltam.z..Da.":0.00061,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":427325.03125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":46.14967,"Ion.Inject.Time..ms.":22,"RT..min.":13.421,"First.Scan":13580,"Last.Scan":13580,"Master.Scan.s.":13578,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_05.raw","File.ID":"F29","Quan.Info":"","Peptides.Matched":360,"XCorr":3.19,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":6.108e-06,"Percolator.SVMScore":2.297,"Precursor.Area":974435.625,"Apex.RT..min.":13.44,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (H4)","Search.ID":"H","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MGSINNNFNTNNNSNTDLDRDWK","Annotated.Sequence":"[-].mGSINNNFNTNNNsNTDLDRDWK.[T]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S14(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P39517","Master.Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","Protein.Accessions":"P39517","Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0616,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":892.37472,"Contaminant":"False","Aligned.m.z..Da.":892.37487,"MH...Da.":2675.10963,"Theo..MH...Da.":2675.11179,"DeltaM..ppm.":-0.81,"Deltam.z..Da.":-0.00072,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":297051.8125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":7.68571,"Ion.Inject.Time..ms.":22,"RT..min.":13.4318,"First.Scan":13594,"Last.Scan":13594,"Master.Scan.s.":13591,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_05.raw","File.ID":"F29","Quan.Info":"","Peptides.Matched":2120,"XCorr":4.87,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":2.29e-10,"Percolator.SVMScore":4.586,"Precursor.Area":2013826.875,"Apex.RT..min.":13.47,"ptmRS..Binomial.Peptide.Score":564.53,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(12): 1459.66; y1+-H(3) O(4) P(11): 1345.61; y1+-H(3) O(4) P(10): 1231.57; b2+-H(3) O(4) P(21): 1122.98; y2+-H(3) O(4) P(17): 1025.45; y2+-H(3) O(4) P(16): 968.43; y2+-H(3) O(4) P(15): 894.90; y2+-H(3) O(4) P(14): 837.88; y2+-H(3) O(4) P(13): 787.35; y2+-H(3) O(4) P(12): 730.33; y2+-H(3) O(4) P(11): 673.31; y2+-H(3) O(4) P(10): 616.29; y1+(9): 1162.55; y1+(8): 1048.51","ptmRS..Best.Site.Probabilities":"S14(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(3): 0.0; T(10): 0.0; S(14): 100.0; T(16): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (H4)","Search.ID":"H","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"MGSINNNFNTNNNSNTDLDRDWK","Annotated.Sequence":"[-].mGSINNNFNTNNNsNTDLDRDWK.[T]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S14(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P39517","Master.Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","Protein.Accessions":"P39517","Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.0497,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1338.05468,"Contaminant":"False","Aligned.m.z..Da.":1338.05491,"MH...Da.":2675.10209,"Theo..MH...Da.":2675.11179,"DeltaM..ppm.":-3.63,"Deltam.z..Da.":-0.00485,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":276474.8125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":13.4643,"First.Scan":13636,"Last.Scan":13636,"Master.Scan.s.":13630,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_05.raw","File.ID":"F29","Quan.Info":"","Peptides.Matched":2000,"XCorr":3.82,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":2.622e-07,"Percolator.SVMScore":3.004,"Precursor.Area":226585.125,"Apex.RT..min.":13.47,"ptmRS..Binomial.Peptide.Score":421.55,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(12): 1459.66; y1+-H(3) O(4) P(10): 1231.57; y2+-H(3) O(4) P(18): 1082.48; y2+-H(3) O(4) P(15): 894.90; y1+(9): 1162.55; y1+(8): 1048.51","ptmRS..Best.Site.Probabilities":"S14(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(3): 0.0; T(10): 0.0; S(14): 100.0; T(16): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (H4)","Search.ID":"H","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"TGTFSIAALQR","Annotated.Sequence":"[K].TGTFSIAALQR.[I]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7297,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":582.82299,"Contaminant":"False","Aligned.m.z..Da.":582.82309,"MH...Da.":1164.63871,"Theo..MH...Da.":1164.63715,"DeltaM..ppm.":1.34,"Deltam.z..Da.":0.00078,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":393064.15625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":31.49536,"Ion.Inject.Time..ms.":22,"RT..min.":13.4751,"First.Scan":13650,"Last.Scan":13650,"Master.Scan.s.":13643,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_05.raw","File.ID":"F29","Quan.Info":"","Peptides.Matched":360,"XCorr":2.22,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":0.00015,"Percolator.SVMScore":1.56,"Precursor.Area":974435.625,"Apex.RT..min.":13.44,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (H4)","Search.ID":"H","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEySDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); Y12(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.3736,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40454,"Contaminant":"False","Aligned.m.z..Da.":967.40457,"MH...Da.":2900.19909,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":-0.08,"Deltam.z..Da.":-0.00008,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":302781.0625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":7.41821,"Ion.Inject.Time..ms.":22,"RT..min.":14.7358,"First.Scan":15168,"Last.Scan":15168,"Master.Scan.s.":15163,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_05.raw","File.ID":"F29","Quan.Info":"","Peptides.Matched":2503,"XCorr":4.55,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":4.9e-07,"Percolator.SVMScore":2.864,"Precursor.Area":18717604,"Apex.RT..min.":14.97,"ptmRS..Binomial.Peptide.Score":352.47,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(14): 1621.73; y2+-H(3) O(4) P(14): 811.37; b1+(12): 1344.56","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (H4)","Search.ID":"H","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].mSEGITDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6394,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1056.4847,"Contaminant":"False","Aligned.m.z..Da.":1056.48478,"MH...Da.":2111.96213,"Theo..MH...Da.":2111.96151,"DeltaM..ppm.":0.29,"Deltam.z..Da.":0.00031,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":522176.09375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":88.84383,"Ion.Inject.Time..ms.":22,"RT..min.":14.5928,"First.Scan":15006,"Last.Scan":15006,"Master.Scan.s.":14996,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_05.raw","File.ID":"F29","Quan.Info":"","Peptides.Matched":2330,"XCorr":3.55,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":2.335e-08,"Percolator.SVMScore":3.547,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (H4)","Search.ID":"H","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].mSEGITDIEEsQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S11(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1398,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1096.46509,"Contaminant":"False","Aligned.m.z..Da.":1096.46507,"MH...Da.":2191.92291,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":-2.25,"Deltam.z..Da.":-0.00247,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":401547.78125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":14.887,"First.Scan":15362,"Last.Scan":15362,"Master.Scan.s.":15360,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_05.raw","File.ID":"F29","Quan.Info":"","Peptides.Matched":1912,"XCorr":2.36,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":0.00022,"Percolator.SVMScore":1.466,"ptmRS..Binomial.Peptide.Score":260.67,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(14): 1665.76; y1+-H(3) O(4) P(11): 1336.60; y1+-H(3) O(4) P(10): 1207.56; b1+(6): 530.25; b1+(8): 758.36","ptmRS..Best.Site.Probabilities":"S11(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; T(6): 0.0; S(11): 100.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (H4)","Search.ID":"H","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FDDMELDENLLR","Annotated.Sequence":"[K].FDDmELDENLLR.[G]","Modifications":"M4(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5246,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":763.34462,"Contaminant":"False","Aligned.m.z..Da.":763.34469,"MH...Da.":1525.68196,"Theo..MH...Da.":1525.6839,"DeltaM..ppm.":-1.27,"Deltam.z..Da.":-0.00097,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":390368.625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":33.39305,"Ion.Inject.Time..ms.":22,"RT..min.":14.495,"First.Scan":14882,"Last.Scan":14882,"Master.Scan.s.":14877,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_05.raw","File.ID":"F29","Quan.Info":"","Peptides.Matched":971,"XCorr":2.84,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":8.165e-06,"Percolator.SVMScore":2.231,"Precursor.Area":1755925,"Apex.RT..min.":14.56,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (H4)","Search.ID":"H","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FDDMELDENLLR","Annotated.Sequence":"[K].FDDmELDENLLR.[G]","Modifications":"M4(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":3,"DeltaScore":0.6311,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":763.34535,"Contaminant":"False","Aligned.m.z..Da.":763.34542,"MH...Da.":1525.68343,"Theo..MH...Da.":1525.6839,"DeltaM..ppm.":-0.31,"Deltam.z..Da.":-0.00024,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2012627.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":41.06075,"Ion.Inject.Time..ms.":22,"RT..min.":14.5607,"First.Scan":14964,"Last.Scan":14964,"Master.Scan.s.":14957,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_05.raw","File.ID":"F29","Quan.Info":"","Peptides.Matched":1072,"XCorr":3.28,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":8.455e-07,"Percolator.SVMScore":2.741,"Precursor.Area":1755925,"Apex.RT..min.":14.56,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (H4)","Search.ID":"H","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KFQDDTLNR","Annotated.Sequence":"[K].KFQDDtLNR.[V]","Modifications":"T6(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7148,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":608.77085,"Contaminant":"False","Aligned.m.z..Da.":608.77085,"MH...Da.":1216.53442,"Theo..MH...Da.":1216.53579,"DeltaM..ppm.":-1.13,"Deltam.z..Da.":-0.00069,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2014859.625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":17.0054,"Ion.Inject.Time..ms.":22,"RT..min.":6.2474,"First.Scan":3868,"Last.Scan":3868,"Master.Scan.s.":3859,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_05.raw","File.ID":"F29","Quan.Info":"","Peptides.Matched":628,"XCorr":2.91,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":0.00024,"Percolator.SVMScore":1.452,"Precursor.Area":3158792.75,"Apex.RT..min.":6.26,"ptmRS..Binomial.Peptide.Score":332.38,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"T6(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"T(6): 100.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (H4)","Search.ID":"H","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DIFSNDELLSDAYDAK","Annotated.Sequence":"[K].DIFSNDELLSDAYDAK.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6998,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":908.41794,"Contaminant":"False","Aligned.m.z..Da.":908.41773,"MH...Da.":1815.82861,"Theo..MH...Da.":1815.82831,"DeltaM..ppm.":0.16,"Deltam.z..Da.":0.00015,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1962281.875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":17.0904,"First.Scan":17857,"Last.Scan":17857,"Master.Scan.s.":17853,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_05.raw","File.ID":"F29","Quan.Info":"","Peptides.Matched":1552,"XCorr":4.13,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":4.039e-09,"Percolator.SVMScore":3.941,"Precursor.Area":2294384,"Apex.RT..min.":17.1,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AI4)","Search.ID":"AI","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"HSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[R].HSDSySENETNHTNVPISSTGGTNNK.[T]","Modifications":"Y5(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0145,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":957.40099,"Contaminant":"False","Aligned.m.z..Da.":957.40108,"MH...Da.":2870.18841,"Theo..MH...Da.":2870.18608,"DeltaM..ppm.":0.81,"Deltam.z..Da.":0.00078,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1417558.375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":7.6517,"First.Scan":5319,"Last.Scan":5319,"Master.Scan.s.":5316,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y25_02.raw","File.ID":"F2","Quan.Info":"","Peptides.Matched":2537,"XCorr":5.53,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":1.482e-12,"Percolator.SVMScore":4.994,"Precursor.Area":1647423,"Apex.RT..min.":7.66,"ptmRS..Binomial.Peptide.Score":279.8,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(4): 409.15; b1+-H(3) O(4) P(5): 572.21; b1+-H(3) O(4) P(6): 659.24; b1+-H(3) O(4) P(11): 1246.46; b1+-H(3) O(4) P(15): 1697.68; b2+-H(3) O(4) P(12): 692.26; b2+-H(3) O(4) P(13): 742.79; b2+-H(3) O(4) P(14): 799.81; b2+-H(3) O(4) P(15): 849.34; y2+-H(3) O(4) P(25): 1318.08; y2+-H(3) O(4) P(23): 1217.05","ptmRS..Best.Site.Probabilities":"S4(Phospho): 99.33","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(4): 99.3; Y(5): 0.0; S(6): 0.7; T(10): 0.0; T(13): 0.0; S(18): 0.0; S(19): 0.0; T(20): 0.0; T(23): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AI4)","Search.ID":"AI","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"HSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[R].HSDSySENETNHTNVPISSTGGTNNK.[T]","Modifications":"Y5(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":0,"Charge":4,"Original.Precursor.Charge":4,"DeltaScore":0.0082,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":718.30235,"Contaminant":"False","Aligned.m.z..Da.":718.30242,"MH...Da.":2870.18759,"Theo..MH...Da.":2870.18608,"DeltaM..ppm.":0.53,"Deltam.z..Da.":0.00038,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1208309.625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":6.68749,"Ion.Inject.Time..ms.":22,"RT..min.":7.6592,"First.Scan":5330,"Last.Scan":5330,"Master.Scan.s.":5329,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y25_02.raw","File.ID":"F2","Quan.Info":"","Peptides.Matched":2487,"XCorr":4.88,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":5.332e-09,"Percolator.SVMScore":3.407,"Precursor.Area":893140.9375,"Apex.RT..min.":7.66,"ptmRS..Binomial.Peptide.Score":230.88,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(5): 572.21; b2+-H(3) O(4) P(7): 394.65; b2+-H(3) O(4) P(13): 742.79; b2+-H(3) O(4) P(14): 799.81; b2+-H(3) O(4) P(15): 849.34","ptmRS..Best.Site.Probabilities":"S4(Phospho): 49.43; S6(Phospho): 49.43","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(4): 49.4; Y(5): 0.0; S(6): 49.4; T(10): 0.6; T(13): 0.6; S(18): 0.0; S(19): 0.0; T(20): 0.0; T(23): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AI4)","Search.ID":"AI","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"IQDPQMTGYVSTR","Annotated.Sequence":"[R].IQDPQmTGyVSTR.[Y]","Modifications":"M6(Oxidation); Y9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P32485","Master.Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","Protein.Accessions":"P32485","Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1319,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":796.34511,"Contaminant":"False","Aligned.m.z..Da.":796.34519,"MH...Da.":1591.68296,"Theo..MH...Da.":1591.6822,"DeltaM..ppm.":0.47,"Deltam.z..Da.":0.00038,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1045653.875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":5.62389,"Ion.Inject.Time..ms.":22,"RT..min.":7.6808,"First.Scan":5365,"Last.Scan":5365,"Master.Scan.s.":5355,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y25_02.raw","File.ID":"F2","Quan.Info":"","Peptides.Matched":1253,"XCorr":2.35,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":7.691e-06,"Percolator.SVMScore":1.997,"Precursor.Area":701424.6875,"Apex.RT..min.":7.68,"ptmRS..Binomial.Peptide.Score":242.84,"ptmRS..Isoform.Confidence.Probability":0.499,"ptmRS..Isoform.Group.Report":"b2+-H(3) O(4) P(11): 609.78","ptmRS..Best.Site.Probabilities":"T7(Phospho): 49.87; Y9(Phospho): 49.87","ptmRS..Phospho.Site.Probabilities":"T(7): 49.9; Y(9): 49.9; S(11): 0.3; T(12): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AI4)","Search.ID":"AI","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"TGTFSIAALQR","Annotated.Sequence":"[K].TGTFSIAALQR.[I]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7358,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":582.82235,"Contaminant":"False","Aligned.m.z..Da.":582.82239,"MH...Da.":1164.63742,"Theo..MH...Da.":1164.63715,"DeltaM..ppm.":0.24,"Deltam.z..Da.":0.00014,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":217490.21875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":34.50904,"Ion.Inject.Time..ms.":22,"RT..min.":13.3738,"First.Scan":12649,"Last.Scan":12649,"Master.Scan.s.":12639,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y25_02.raw","File.ID":"F2","Quan.Info":"","Peptides.Matched":361,"XCorr":2.12,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":0.00003,"Percolator.SVMScore":1.707,"Precursor.Area":143772.5,"Apex.RT..min.":13.37,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AI4)","Search.ID":"AI","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"GVFGYGFEEPSAIQQR","Annotated.Sequence":"[R].GVFGYGFEEPSAIQQR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5714,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":892.93168,"Contaminant":"False","Aligned.m.z..Da.":892.93171,"MH...Da.":1784.85609,"Theo..MH...Da.":1784.86022,"DeltaM..ppm.":-2.31,"Deltam.z..Da.":-0.00207,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":290003.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":15.0265,"First.Scan":14456,"Last.Scan":14456,"Master.Scan.s.":14450,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y25_02.raw","File.ID":"F2","Quan.Info":"","Peptides.Matched":1339,"XCorr":2.24,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":4.001e-06,"Percolator.SVMScore":2.123,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AI4)","Search.ID":"AI","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEySDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); Y12(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.4173,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40392,"Contaminant":"False","Aligned.m.z..Da.":967.40395,"MH...Da.":2900.19721,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":-0.73,"Deltam.z..Da.":-0.00071,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":46895.29297,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":20.08773,"Ion.Inject.Time..ms.":22,"RT..min.":15.0384,"First.Scan":14471,"Last.Scan":14471,"Master.Scan.s.":14463,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y25_02.raw","File.ID":"F2","Quan.Info":"","Peptides.Matched":2388,"XCorr":3.81,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":4.055e-09,"Percolator.SVMScore":3.46,"Precursor.Area":3490870,"Apex.RT..min.":15.08,"ptmRS..Binomial.Peptide.Score":364.01,"ptmRS..Isoform.Confidence.Probability":0.004,"ptmRS..Isoform.Group.Report":"y2+-H(3) O(4) P(14): 811.37","ptmRS..Best.Site.Probabilities":"S13(Phospho): 99.55","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.5; S(13): 99.6; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AI4)","Search.ID":"AI","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.398,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40475,"Contaminant":"False","Aligned.m.z..Da.":967.40478,"MH...Da.":2900.19972,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":0.13,"Deltam.z..Da.":0.00013,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":139208.64062,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":19.887,"Ion.Inject.Time..ms.":22,"RT..min.":15.5553,"First.Scan":15071,"Last.Scan":15071,"Master.Scan.s.":15070,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y25_02.raw","File.ID":"F2","Quan.Info":"","Peptides.Matched":2465,"XCorr":3.04,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":2.909e-07,"Percolator.SVMScore":2.632,"Precursor.Area":769312.125,"Apex.RT..min.":15.37,"ptmRS..Binomial.Peptide.Score":321.11,"ptmRS..Isoform.Confidence.Probability":0.994,"ptmRS..Isoform.Group.Report":"b1+(12): 1344.56","ptmRS..Best.Site.Probabilities":"S13(Phospho): 99.35","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.7; S(13): 99.4; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AI4)","Search.ID":"AI","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEySDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); Y12(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":4,"DeltaScore":0.3577,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40565,"Contaminant":"False","Aligned.m.z..Da.":967.40568,"MH...Da.":2900.2024,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":1.06,"Deltam.z..Da.":0.00102,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":619044.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":66.89127,"Ion.Inject.Time..ms.":22,"RT..min.":15.1788,"First.Scan":14643,"Last.Scan":14643,"Master.Scan.s.":14641,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y25_02.raw","File.ID":"F2","Quan.Info":"","Peptides.Matched":2543,"XCorr":3.55,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":8.097e-09,"Percolator.SVMScore":3.326,"Precursor.Area":3490870,"Apex.RT..min.":15.08,"ptmRS..Binomial.Peptide.Score":304.44,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"b2+-H(3) O(4) P(18): 1014.90; y2+-H(3) O(4) P(14): 811.37","ptmRS..Best.Site.Probabilities":"S13(Phospho): 99.99","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AI4)","Search.ID":"AI","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":5,"DeltaScore":0.0802,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40515,"Contaminant":"False","Aligned.m.z..Da.":967.40519,"MH...Da.":2900.20091,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":0.54,"Deltam.z..Da.":0.00053,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1786290.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":16.91906,"Ion.Inject.Time..ms.":22,"RT..min.":15.1206,"First.Scan":14572,"Last.Scan":14572,"Master.Scan.s.":14567,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y25_02.raw","File.ID":"F2","Quan.Info":"","Peptides.Matched":2507,"XCorr":4.74,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":1.604e-13,"Percolator.SVMScore":5.425,"Precursor.Area":3490870,"Apex.RT..min.":15.08,"ptmRS..Binomial.Peptide.Score":678.03,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(13): 1413.58; b1+-H(3) O(4) P(16): 1771.69; y1+-H(3) O(4) P(15): 1750.78; y1+-H(3) O(4) P(14): 1621.73; y1+-H(3) O(4) P(13): 1458.67; b2+-H(3) O(4) P(16): 886.35; b2+-H(3) O(4) P(18): 1014.90; y2+-H(3) O(4) P(14): 811.37; b1+(12): 1344.56; y1+(13): 1556.65","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AI4)","Search.ID":"AI","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].msEGITDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S2(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1064,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1096.46861,"Contaminant":"False","Aligned.m.z..Da.":1096.46864,"MH...Da.":2191.92995,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":0.96,"Deltam.z..Da.":0.00105,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":420020.21875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":8.45353,"Ion.Inject.Time..ms.":22,"RT..min.":15.7316,"First.Scan":15245,"Last.Scan":15245,"Master.Scan.s.":15244,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y25_02.raw","File.ID":"F2","Quan.Info":"","Peptides.Matched":2040,"XCorr":4.23,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":1.01e-09,"Percolator.SVMScore":3.729,"Precursor.Area":2237462.75,"Apex.RT..min.":15.77,"ptmRS..Binomial.Peptide.Score":508.27,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(2): 112.04; b1+-H(3) O(4) P(3): 241.08; b1+-H(3) O(4) P(4): 298.10; b1+-H(3) O(4) P(5): 411.19; b1+-H(3) O(4) P(6): 512.24; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+-H(3) O(4) P(9): 869.39; b1+-H(3) O(4) P(10): 998.43; b1+(4): 396.08; y1+(14): 1683.77","ptmRS..Best.Site.Probabilities":"S2(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 100.0; T(6): 0.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AI4)","Search.ID":"AI","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DIFSNDELLSDAYDAK","Annotated.Sequence":"[K].DIFSNDELLSDAYDAK.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.763,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":908.42151,"Contaminant":"False","Aligned.m.z..Da.":908.42154,"MH...Da.":1815.83576,"Theo..MH...Da.":1815.82831,"DeltaM..ppm.":4.1,"Deltam.z..Da.":0.00372,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":353857.09375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":14.17491,"Ion.Inject.Time..ms.":22,"RT..min.":17.0821,"First.Scan":16649,"Last.Scan":16649,"Master.Scan.s.":16645,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y25_02.raw","File.ID":"F2","Quan.Info":"","Peptides.Matched":1731,"XCorr":3.46,"X..Protein.Groups":1,"Percolator.q.Value":0.00017,"Percolator.PEP":1.685e-08,"Percolator.SVMScore":3.184,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AH4)","Search.ID":"AH","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DIFSNDELLSDAYDAK","Annotated.Sequence":"[K].DIFSNDELLSDAYDAK.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.724,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":908.4149,"Contaminant":"False","Aligned.m.z..Da.":908.41497,"MH...Da.":1815.82252,"Theo..MH...Da.":1815.82831,"DeltaM..ppm.":-3.19,"Deltam.z..Da.":-0.0029,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":230811.39062,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":18.1435,"Ion.Inject.Time..ms.":22,"RT..min.":17.0748,"First.Scan":16813,"Last.Scan":16813,"Master.Scan.s.":16812,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y25_03.raw","File.ID":"F3","Quan.Info":"","Peptides.Matched":1579,"XCorr":3.37,"X..Protein.Groups":1,"Percolator.q.Value":0.00018,"Percolator.PEP":6.166e-08,"Percolator.SVMScore":3.256,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AH4)","Search.ID":"AH","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MGSINNNFNTNNNSNTDLDRDWK","Annotated.Sequence":"[-].mGSINNNFNTNNNsNTDLDRDWK.[T]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S14(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P39517","Master.Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","Protein.Accessions":"P39517","Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0933,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":892.37778,"Contaminant":"False","Aligned.m.z..Da.":892.37779,"MH...Da.":2675.11881,"Theo..MH...Da.":2675.11179,"DeltaM..ppm.":2.62,"Deltam.z..Da.":0.00234,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":208170.26562,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":7.50892,"Ion.Inject.Time..ms.":22,"RT..min.":13.54,"First.Scan":12922,"Last.Scan":12922,"Master.Scan.s.":12912,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y25_03.raw","File.ID":"F3","Quan.Info":"","Peptides.Matched":2393,"XCorr":3,"X..Protein.Groups":1,"Percolator.q.Value":0.00018,"Percolator.PEP":0.00006,"Percolator.SVMScore":1.707,"Precursor.Area":579073.75,"Apex.RT..min.":13.56,"ptmRS..Binomial.Peptide.Score":351.57,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(12): 1459.66; b2+-H(3) O(4) P(21): 1122.98; y2+-H(3) O(4) P(16): 968.43; y2+-H(3) O(4) P(15): 894.90; y2+-H(3) O(4) P(14): 837.88; y2+-H(3) O(4) P(13): 787.35; y2+-H(3) O(4) P(12): 730.33; y2+-H(3) O(4) P(10): 616.29; y1+(9): 1162.55; y1+(8): 1048.51","ptmRS..Best.Site.Probabilities":"S14(Phospho): 99.99","ptmRS..Phospho.Site.Probabilities":"S(3): 0.0; T(10): 0.0; S(14): 100.0; T(16): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AH4)","Search.ID":"AH","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":4,"DeltaScore":0.4237,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40486,"Contaminant":"False","Aligned.m.z..Da.":967.40473,"MH...Da.":2900.20004,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":0.24,"Deltam.z..Da.":0.00024,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":181311.85938,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":77.33665,"Ion.Inject.Time..ms.":22,"RT..min.":15.4661,"First.Scan":15133,"Last.Scan":15133,"Master.Scan.s.":15128,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y25_03.raw","File.ID":"F3","Quan.Info":"","Peptides.Matched":2476,"XCorr":2.36,"X..Protein.Groups":1,"Percolator.q.Value":0.00018,"Percolator.PEP":0.00015,"Percolator.SVMScore":1.474,"Precursor.Area":793973.1875,"Apex.RT..min.":15.37,"ptmRS..Binomial.Peptide.Score":214.17,"ptmRS..Isoform.Confidence.Probability":0.5,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"Y12(Phospho): 50; S13(Phospho): 50","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 50.0; S(13): 50.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AH4)","Search.ID":"AH","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEySDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); Y12(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.3889,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40506,"Contaminant":"False","Aligned.m.z..Da.":967.40493,"MH...Da.":2900.20064,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":0.45,"Deltam.z..Da.":0.00044,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":149539.10938,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":44.92915,"Ion.Inject.Time..ms.":22,"RT..min.":15.6152,"First.Scan":15278,"Last.Scan":15278,"Master.Scan.s.":15271,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y25_03.raw","File.ID":"F3","Quan.Info":"","Peptides.Matched":2486,"XCorr":2.34,"X..Protein.Groups":1,"Percolator.q.Value":0.00018,"Percolator.PEP":3.135e-06,"Percolator.SVMScore":2.363,"Precursor.Area":3550079,"Apex.RT..min.":15.08,"ptmRS..Binomial.Peptide.Score":272.31,"ptmRS..Isoform.Confidence.Probability":0.5,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"Y12(Phospho): 50; S13(Phospho): 50","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 50.0; S(13): 50.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AH4)","Search.ID":"AH","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LQETNPEEVPK","Annotated.Sequence":"[K].LQETNPEEVPK.[F]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.4583,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":642.32823,"Contaminant":"False","Aligned.m.z..Da.":642.3283,"MH...Da.":1283.64918,"Theo..MH...Da.":1283.64777,"DeltaM..ppm.":1.1,"Deltam.z..Da.":0.00071,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1447911.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":3.91719,"Ion.Inject.Time..ms.":22,"RT..min.":7.6494,"First.Scan":5366,"Last.Scan":5366,"Master.Scan.s.":5357,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y25_03.raw","File.ID":"F3","Quan.Info":"","Peptides.Matched":521,"XCorr":2.4,"X..Protein.Groups":1,"Percolator.q.Value":0.00018,"Percolator.PEP":0.00011,"Percolator.SVMScore":1.546,"Precursor.Area":1137655.5,"Apex.RT..min.":7.65,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AH4)","Search.ID":"AH","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"HSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[R].HSDSYsENETNHTNVPISSTGGTNNK.[T]","Modifications":"S6(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":0,"Charge":4,"Original.Precursor.Charge":4,"DeltaScore":0.0026,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":718.30142,"Contaminant":"False","Aligned.m.z..Da.":718.3015,"MH...Da.":2870.18385,"Theo..MH...Da.":2870.18608,"DeltaM..ppm.":-0.78,"Deltam.z..Da.":-0.00056,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":882007.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":6.06184,"Ion.Inject.Time..ms.":22,"RT..min.":7.6483,"First.Scan":5364,"Last.Scan":5364,"Master.Scan.s.":5357,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y25_03.raw","File.ID":"F3","Quan.Info":"","Peptides.Matched":2449,"XCorr":3.85,"X..Protein.Groups":1,"Percolator.q.Value":0.00018,"Percolator.PEP":3.469e-07,"Percolator.SVMScore":2.863,"ptmRS..Binomial.Peptide.Score":327.05,"ptmRS..Isoform.Confidence.Probability":0.332,"ptmRS..Isoform.Group.Report":"b2+-H(3) O(4) P(14): 799.81; b2+-H(3) O(4) P(15): 849.34; b2+-H(3) O(4) P(17): 954.41","ptmRS..Best.Site.Probabilities":"S6(Phospho): 33.23; T10(Phospho): 33.23; T13(Phospho): 33.23","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(4): 0.3; Y(5): 0.0; S(6): 33.2; T(10): 33.2; T(13): 33.2; S(18): 0.0; S(19): 0.0; T(20): 0.0; T(23): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AH4)","Search.ID":"AH","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"HSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[R].HSDSySENETNHTNVPISSTGGTNNK.[T]","Modifications":"Y5(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0129,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":957.40088,"Contaminant":"False","Aligned.m.z..Da.":957.40098,"MH...Da.":2870.18809,"Theo..MH...Da.":2870.18608,"DeltaM..ppm.":0.7,"Deltam.z..Da.":0.00067,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1959219.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":7.6384,"First.Scan":5349,"Last.Scan":5349,"Master.Scan.s.":5344,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y25_03.raw","File.ID":"F3","Quan.Info":"","Peptides.Matched":2527,"XCorr":6.18,"X..Protein.Groups":1,"Percolator.q.Value":0.00018,"Percolator.PEP":7.35e-13,"Percolator.SVMScore":5.834,"Precursor.Area":1882202.875,"Apex.RT..min.":7.64,"ptmRS..Binomial.Peptide.Score":294.64,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(4): 409.15; b1+-H(3) O(4) P(5): 572.21; b1+-H(3) O(4) P(6): 659.24; b1+-H(3) O(4) P(9): 1031.37; b1+-H(3) O(4) P(11): 1246.46; b1+-H(3) O(4) P(15): 1697.68; b2+-H(3) O(4) P(13): 742.79; b2+-H(3) O(4) P(14): 799.81; b2+-H(3) O(4) P(15): 849.34; y2+-H(3) O(4) P(25): 1318.08; y2+-H(3) O(4) P(24): 1274.56; y2+-H(3) O(4) P(23): 1217.05","ptmRS..Best.Site.Probabilities":"S4(Phospho): 99.36","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(4): 99.4; Y(5): 0.0; S(6): 0.6; T(10): 0.0; T(13): 0.0; S(18): 0.0; S(19): 0.0; T(20): 0.0; T(23): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AH4)","Search.ID":"AH","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEySDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); Y12(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":4,"DeltaScore":0.3442,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40513,"Contaminant":"False","Aligned.m.z..Da.":967.40502,"MH...Da.":2900.20084,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":0.52,"Deltam.z..Da.":0.0005,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":728213.9375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":67.21894,"Ion.Inject.Time..ms.":22,"RT..min.":15.1724,"First.Scan":14782,"Last.Scan":14782,"Master.Scan.s.":14777,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y25_03.raw","File.ID":"F3","Quan.Info":"","Peptides.Matched":2521,"XCorr":3.37,"X..Protein.Groups":1,"Percolator.q.Value":0.00018,"Percolator.PEP":2.559e-07,"Percolator.SVMScore":2.933,"Precursor.Area":3550079,"Apex.RT..min.":15.08,"ptmRS..Binomial.Peptide.Score":262.34,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"y2+-H(3) O(4) P(14): 811.37; b1+(12): 1344.56","ptmRS..Best.Site.Probabilities":"S13(Phospho): 99.99","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AH4)","Search.ID":"AH","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":5,"DeltaScore":0.0931,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40493,"Contaminant":"False","Aligned.m.z..Da.":967.40483,"MH...Da.":2900.20025,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":0.32,"Deltam.z..Da.":0.00031,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1190383.125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":20.9019,"Ion.Inject.Time..ms.":22,"RT..min.":15.1414,"First.Scan":14743,"Last.Scan":14743,"Master.Scan.s.":14738,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y25_03.raw","File.ID":"F3","Quan.Info":"","Peptides.Matched":2490,"XCorr":6.34,"X..Protein.Groups":1,"Percolator.q.Value":0.00018,"Percolator.PEP":1.154e-12,"Percolator.SVMScore":5.732,"Precursor.Area":3550079,"Apex.RT..min.":15.08,"ptmRS..Binomial.Peptide.Score":616.84,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(13): 1413.58; b1+-H(3) O(4) P(14): 1528.61; b1+-H(3) O(4) P(16): 1771.69; y1+-H(3) O(4) P(15): 1750.78; y1+-H(3) O(4) P(14): 1621.73; b2+-H(3) O(4) P(16): 886.35; b2+-H(3) O(4) P(18): 1014.90; y2+-H(3) O(4) P(14): 811.37; b1+(12): 1344.56; y1+(13): 1556.65","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AH4)","Search.ID":"AH","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.4253,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40665,"Contaminant":"False","Aligned.m.z..Da.":967.40656,"MH...Da.":2900.2054,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":2.09,"Deltam.z..Da.":0.00202,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":221338.85938,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":15.033,"First.Scan":14610,"Last.Scan":14610,"Master.Scan.s.":14608,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y25_03.raw","File.ID":"F3","Quan.Info":"","Peptides.Matched":2573,"XCorr":3.88,"X..Protein.Groups":1,"Percolator.q.Value":0.00018,"Percolator.PEP":1.12e-06,"Percolator.SVMScore":2.597,"Precursor.Area":3550079,"Apex.RT..min.":15.08,"ptmRS..Binomial.Peptide.Score":309.14,"ptmRS..Isoform.Confidence.Probability":0.5,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"Y12(Phospho): 50; S13(Phospho): 50","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 50.0; S(13): 50.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AH4)","Search.ID":"AH","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MGSINNNFNTNNNSNTDLDRDWK","Annotated.Sequence":"[-].mGSINNNFNTNNNsNTDLDRDWK.[T]","Modifications":"N-Term(Prot)(Met-loss); S14(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P39517","Master.Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","Protein.Accessions":"P39517","Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0168,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":878.37029,"Contaminant":"False","Aligned.m.z..Da.":878.37037,"MH...Da.":2633.09632,"Theo..MH...Da.":2633.10123,"DeltaM..ppm.":-1.86,"Deltam.z..Da.":-0.00163,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":695942.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":8.30047,"Ion.Inject.Time..ms.":22,"RT..min.":11.3212,"First.Scan":10093,"Last.Scan":10093,"Master.Scan.s.":10089,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y25_03.raw","File.ID":"F3","Quan.Info":"","Peptides.Matched":2162,"XCorr":4.17,"X..Protein.Groups":1,"Percolator.q.Value":0.00018,"Percolator.PEP":1.814e-08,"Percolator.SVMScore":3.534,"Precursor.Area":586946.9375,"Apex.RT..min.":11.33,"ptmRS..Binomial.Peptide.Score":415.71,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(11): 1345.61; y1+-H(3) O(4) P(10): 1231.57; b2+-H(3) O(4) P(15): 744.32; y2+-H(3) O(4) P(17): 1025.45; y2+-H(3) O(4) P(16): 968.43; y2+-H(3) O(4) P(15): 894.90; y2+-H(3) O(4) P(14): 837.88; y2+-H(3) O(4) P(13): 787.35; y2+-H(3) O(4) P(12): 730.33; y2+-H(3) O(4) P(11): 673.31; y1+(9): 1162.55","ptmRS..Best.Site.Probabilities":"S14(Phospho): 99.99","ptmRS..Phospho.Site.Probabilities":"S(3): 0.0; T(10): 0.0; S(14): 100.0; T(16): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AH4)","Search.ID":"AH","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"IQDPQMTGYVSTR","Annotated.Sequence":"[R].IQDPQMTGyVSTR.[Y]","Modifications":"Y9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P32485","Master.Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","Protein.Accessions":"P32485","Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1107,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":788.34589,"Contaminant":"False","Aligned.m.z..Da.":788.34587,"MH...Da.":1575.6845,"Theo..MH...Da.":1575.68728,"DeltaM..ppm.":-1.76,"Deltam.z..Da.":-0.00139,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1050602.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":22.91183,"Ion.Inject.Time..ms.":22,"RT..min.":9.5699,"First.Scan":7828,"Last.Scan":7828,"Master.Scan.s.":7827,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y25_03.raw","File.ID":"F3","Quan.Info":"","Peptides.Matched":1146,"XCorr":2.44,"X..Protein.Groups":1,"Percolator.q.Value":0.00018,"Percolator.PEP":6.001e-06,"Percolator.SVMScore":2.215,"Precursor.Area":808400.8125,"Apex.RT..min.":9.56,"ptmRS..Binomial.Peptide.Score":264.44,"ptmRS..Isoform.Confidence.Probability":0.985,"ptmRS..Isoform.Group.Report":"y1+(6): 762.32; y1+(5): 705.30","ptmRS..Best.Site.Probabilities":"Y9(Phospho): 98.5","ptmRS..Phospho.Site.Probabilities":"T(7): 0.5; Y(9): 98.5; S(11): 0.5; T(12): 0.5"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AH4)","Search.ID":"AH","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].msEGITDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S2(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":3,"DeltaScore":0.1421,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1096.46428,"Contaminant":"False","Aligned.m.z..Da.":1096.46413,"MH...Da.":2191.92127,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":-3,"Deltam.z..Da.":-0.00329,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":840596.125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":26.3469,"Ion.Inject.Time..ms.":22,"RT..min.":15.8179,"First.Scan":15513,"Last.Scan":15513,"Master.Scan.s.":15503,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y25_03.raw","File.ID":"F3","Quan.Info":"","Peptides.Matched":1876,"XCorr":3.66,"X..Protein.Groups":1,"Percolator.q.Value":0.00018,"Percolator.PEP":2.794e-08,"Percolator.SVMScore":3.436,"Precursor.Area":2286270.25,"Apex.RT..min.":15.77,"ptmRS..Binomial.Peptide.Score":388.8,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(2): 112.04; b1+-H(3) O(4) P(3): 241.08; b1+-H(3) O(4) P(4): 298.10; b1+-H(3) O(4) P(5): 411.19; b1+-H(3) O(4) P(6): 512.24; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+-H(3) O(4) P(9): 869.39; b1+-H(3) O(4) P(10): 998.43","ptmRS..Best.Site.Probabilities":"S2(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 100.0; T(6): 0.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AH4)","Search.ID":"AH","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].msEGITDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S2(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.087,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":731.31459,"Contaminant":"False","Aligned.m.z..Da.":731.31449,"MH...Da.":2191.92921,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":0.63,"Deltam.z..Da.":0.00046,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":228120.84375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":4.68065,"Ion.Inject.Time..ms.":22,"RT..min.":15.7642,"First.Scan":15445,"Last.Scan":15445,"Master.Scan.s.":15438,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y25_03.raw","File.ID":"F3","Quan.Info":"","Peptides.Matched":1986,"XCorr":2.99,"X..Protein.Groups":1,"Percolator.q.Value":0.00018,"Percolator.PEP":0.00021,"Percolator.SVMScore":1.402,"Precursor.Area":346186.8125,"Apex.RT..min.":15.77,"ptmRS..Binomial.Peptide.Score":314.65,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(2): 112.04; b1+-H(3) O(4) P(3): 241.08; b1+-H(3) O(4) P(4): 298.10; b1+-H(3) O(4) P(5): 411.19; b1+-H(3) O(4) P(6): 512.24; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+-H(3) O(4) P(9): 869.39","ptmRS..Best.Site.Probabilities":"S2(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 100.0; T(6): 0.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AH4)","Search.ID":"AH","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].msEGITDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S2(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1737,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1096.46892,"Contaminant":"False","Aligned.m.z..Da.":1096.46877,"MH...Da.":2191.93057,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":1.25,"Deltam.z..Da.":0.00136,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":416601.40625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":15.7374,"First.Scan":15416,"Last.Scan":15416,"Master.Scan.s.":15413,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y25_03.raw","File.ID":"F3","Quan.Info":"","Peptides.Matched":2096,"XCorr":4.03,"X..Protein.Groups":1,"Percolator.q.Value":0.00018,"Percolator.PEP":6.731e-08,"Percolator.SVMScore":3.236,"Precursor.Area":2286270.25,"Apex.RT..min.":15.77,"ptmRS..Binomial.Peptide.Score":508.27,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(2): 112.04; b1+-H(3) O(4) P(3): 241.08; b1+-H(3) O(4) P(4): 298.10; b1+-H(3) O(4) P(5): 411.19; b1+-H(3) O(4) P(6): 512.24; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+-H(3) O(4) P(9): 869.39; b1+-H(3) O(4) P(10): 998.43; b1+(4): 396.08; y1+(14): 1683.77","ptmRS..Best.Site.Probabilities":"S2(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 100.0; T(6): 0.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AH4)","Search.ID":"AH","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"GVFGYGFEEPSAIQQR","Annotated.Sequence":"[R].GVFGYGFEEPSAIQQR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5889,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":892.93218,"Contaminant":"False","Aligned.m.z..Da.":892.9321,"MH...Da.":1784.85709,"Theo..MH...Da.":1784.86022,"DeltaM..ppm.":-1.76,"Deltam.z..Da.":-0.00157,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":170211.71875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":37.93135,"Ion.Inject.Time..ms.":22,"RT..min.":15.0246,"First.Scan":14600,"Last.Scan":14600,"Master.Scan.s.":14595,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y25_03.raw","File.ID":"F3","Quan.Info":"","Peptides.Matched":1388,"XCorr":2.7,"X..Protein.Groups":1,"Percolator.q.Value":0.00018,"Percolator.PEP":0.00002,"Percolator.SVMScore":1.963,"Precursor.Area":119779.25781,"Apex.RT..min.":15.03,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AH4)","Search.ID":"AH","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"APQALMLAPTR","Annotated.Sequence":"[K].APQALMLAPTR.[E]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":3,"DeltaScore":0.6609,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":584.83013,"Contaminant":"False","Aligned.m.z..Da.":584.83016,"MH...Da.":1168.65299,"Theo..MH...Da.":1168.65069,"DeltaM..ppm.":1.97,"Deltam.z..Da.":0.00115,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":214684.60938,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":88.118,"Ion.Inject.Time..ms.":22,"RT..min.":11.6283,"First.Scan":10499,"Last.Scan":10499,"Master.Scan.s.":10492,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y25_03.raw","File.ID":"F3","Quan.Info":"","Peptides.Matched":211,"XCorr":1.74,"X..Protein.Groups":1,"Percolator.q.Value":0.00018,"Percolator.PEP":0.00008,"Percolator.SVMScore":1.639,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AA4)","Search.ID":"AA","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LQETNPEEVPK","Annotated.Sequence":"[K].LQETNPEEVPK.[F]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5475,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":642.32843,"Contaminant":"False","Aligned.m.z..Da.":642.32845,"MH...Da.":1283.64958,"Theo..MH...Da.":1283.64777,"DeltaM..ppm.":1.41,"Deltam.z..Da.":0.0009,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1840931.625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":5.86549,"Ion.Inject.Time..ms.":22,"RT..min.":7.6465,"First.Scan":5492,"Last.Scan":5492,"Master.Scan.s.":5483,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_04.raw","File.ID":"F10","Quan.Info":"","Peptides.Matched":510,"XCorr":2.63,"X..Protein.Groups":1,"Percolator.q.Value":0.0002,"Percolator.PEP":0.00002,"Percolator.SVMScore":1.77,"Precursor.Area":2387921,"Apex.RT..min.":7.65,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AA4)","Search.ID":"AA","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"IQDPQMTGYVSTR","Annotated.Sequence":"[R].IQDPQmTGyVSTR.[Y]","Modifications":"M6(Oxidation); Y9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P32485","Master.Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","Protein.Accessions":"P32485","Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1308,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":796.34566,"Contaminant":"False","Aligned.m.z..Da.":796.34569,"MH...Da.":1591.68404,"Theo..MH...Da.":1591.6822,"DeltaM..ppm.":1.15,"Deltam.z..Da.":0.00092,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1776690.375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":7.6927,"First.Scan":5558,"Last.Scan":5558,"Master.Scan.s.":5548,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_04.raw","File.ID":"F10","Quan.Info":"","Peptides.Matched":1113,"XCorr":3.21,"X..Protein.Groups":1,"Percolator.q.Value":0.0002,"Percolator.PEP":3.47e-07,"Percolator.SVMScore":2.492,"Precursor.Area":1816521.125,"Apex.RT..min.":7.68,"ptmRS..Binomial.Peptide.Score":241.49,"ptmRS..Isoform.Confidence.Probability":0.989,"ptmRS..Isoform.Group.Report":"y1+(6): 762.32; y1+(5): 705.30","ptmRS..Best.Site.Probabilities":"Y9(Phospho): 98.9","ptmRS..Phospho.Site.Probabilities":"T(7): 0.6; Y(9): 98.9; S(11): 0.6; T(12): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AA4)","Search.ID":"AA","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"IQDPQMTGYVSTR","Annotated.Sequence":"[R].IQDPQMTGyVSTR.[Y]","Modifications":"Y9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P32485","Master.Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","Protein.Accessions":"P32485","Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1084,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":788.34536,"Contaminant":"False","Aligned.m.z..Da.":788.34529,"MH...Da.":1575.68344,"Theo..MH...Da.":1575.68728,"DeltaM..ppm.":-2.44,"Deltam.z..Da.":-0.00192,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2296416.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":8.15484,"Ion.Inject.Time..ms.":22,"RT..min.":9.5638,"First.Scan":7788,"Last.Scan":7788,"Master.Scan.s.":7784,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_04.raw","File.ID":"F10","Quan.Info":"","Peptides.Matched":1106,"XCorr":2.86,"X..Protein.Groups":1,"Percolator.q.Value":0.0002,"Percolator.PEP":0.00001,"Percolator.SVMScore":1.804,"Precursor.Area":1488068.125,"Apex.RT..min.":9.56,"ptmRS..Binomial.Peptide.Score":279.05,"ptmRS..Isoform.Confidence.Probability":0.988,"ptmRS..Isoform.Group.Report":"y1+(6): 762.32; y1+(5): 705.30","ptmRS..Best.Site.Probabilities":"Y9(Phospho): 98.82","ptmRS..Phospho.Site.Probabilities":"T(7): 0.4; Y(9): 98.8; S(11): 0.4; T(12): 0.4"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AA4)","Search.ID":"AA","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"APQALMLAPTR","Annotated.Sequence":"[K].APQALmLAPTR.[E]","Modifications":"M6(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6557,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":592.82626,"Contaminant":"False","Aligned.m.z..Da.":592.82622,"MH...Da.":1184.64525,"Theo..MH...Da.":1184.6456,"DeltaM..ppm.":-0.3,"Deltam.z..Da.":-0.00018,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1224669.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":19.41327,"Ion.Inject.Time..ms.":22,"RT..min.":10.2053,"First.Scan":8566,"Last.Scan":8566,"Master.Scan.s.":8564,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_04.raw","File.ID":"F10","Quan.Info":"","Peptides.Matched":325,"XCorr":2.44,"X..Protein.Groups":1,"Percolator.q.Value":0.0002,"Percolator.PEP":0.00005,"Percolator.SVMScore":1.539,"Precursor.Area":982761.5625,"Apex.RT..min.":10.19,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AA4)","Search.ID":"AA","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"HSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[R].HSDSySENETNHTNVPISSTGGTNNK.[T]","Modifications":"Y5(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":0,"Charge":4,"Original.Precursor.Charge":4,"DeltaScore":0.006,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":718.30305,"Contaminant":"False","Aligned.m.z..Da.":718.30308,"MH...Da.":2870.1904,"Theo..MH...Da.":2870.18608,"DeltaM..ppm.":1.5,"Deltam.z..Da.":0.00108,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1368267.375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":7.6459,"First.Scan":5491,"Last.Scan":5491,"Master.Scan.s.":5483,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_04.raw","File.ID":"F10","Quan.Info":"","Peptides.Matched":2558,"XCorr":5.03,"X..Protein.Groups":1,"Percolator.q.Value":0.0002,"Percolator.PEP":6.38e-09,"Percolator.SVMScore":3.253,"Precursor.Area":2579523,"Apex.RT..min.":7.65,"ptmRS..Binomial.Peptide.Score":269.15,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(11): 1246.46; b2+-H(3) O(4) P(8): 451.67; b2+-H(3) O(4) P(13): 742.79; b2+-H(3) O(4) P(14): 799.81; b2+-H(3) O(4) P(15): 849.34; b2+-H(3) O(4) P(17): 954.41; b1+(4): 427.16","ptmRS..Best.Site.Probabilities":"S6(Phospho): 98.53","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(4): 0.7; Y(5): 0.0; S(6): 98.5; T(10): 0.7; T(13): 0.0; S(18): 0.0; S(19): 0.0; T(20): 0.0; T(23): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AA4)","Search.ID":"AA","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"HSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[R].HSDSYsENETNHTNVPISSTGGTNNK.[T]","Modifications":"S6(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0074,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":957.40086,"Contaminant":"False","Aligned.m.z..Da.":957.40089,"MH...Da.":2870.18803,"Theo..MH...Da.":2870.18608,"DeltaM..ppm.":0.68,"Deltam.z..Da.":0.00065,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1838255,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":7.6448,"First.Scan":5489,"Last.Scan":5489,"Master.Scan.s.":5483,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_04.raw","File.ID":"F10","Quan.Info":"","Peptides.Matched":2519,"XCorr":6.77,"X..Protein.Groups":1,"Percolator.q.Value":0.0002,"Percolator.PEP":1.396e-13,"Percolator.SVMScore":5.297,"Precursor.Area":3591533.5,"Apex.RT..min.":7.65,"ptmRS..Binomial.Peptide.Score":533.46,"ptmRS..Isoform.Confidence.Probability":0.993,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(6): 659.24; b1+-H(3) O(4) P(9): 1031.37; b1+-H(3) O(4) P(11): 1246.46; b1+-H(3) O(4) P(12): 1383.52; b1+-H(3) O(4) P(15): 1697.68; b2+-H(3) O(4) P(12): 692.26; b2+-H(3) O(4) P(13): 742.79; b2+-H(3) O(4) P(14): 799.81; b2+-H(3) O(4) P(15): 849.34; y2+-H(3) O(4) P(25): 1318.08; y2+-H(3) O(4) P(23): 1217.05; y2+-H(3) O(4) P(21): 1092.00; b1+(4): 427.16","ptmRS..Best.Site.Probabilities":"S6(Phospho): 99.32","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(4): 0.7; Y(5): 0.0; S(6): 99.3; T(10): 0.0; T(13): 0.0; S(18): 0.0; S(19): 0.0; T(20): 0.0; T(23): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AA4)","Search.ID":"AA","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KDELTLEGIK","Annotated.Sequence":"[K].KDELTLEGIK.[Q]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.4959,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":573.32486,"Contaminant":"False","Aligned.m.z..Da.":573.32483,"MH...Da.":1145.64245,"Theo..MH...Da.":1145.64123,"DeltaM..ppm.":1.07,"Deltam.z..Da.":0.00061,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1683177.375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":6.32438,"Ion.Inject.Time..ms.":22,"RT..min.":10.2604,"First.Scan":8635,"Last.Scan":8635,"Master.Scan.s.":8629,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_04.raw","File.ID":"F10","Quan.Info":"","Peptides.Matched":301,"XCorr":3.63,"X..Protein.Groups":1,"Percolator.q.Value":0.0002,"Percolator.PEP":4.88e-09,"Percolator.SVMScore":3.304,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AA4)","Search.ID":"AA","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":5,"DeltaScore":0.0388,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40428,"Contaminant":"False","Aligned.m.z..Da.":967.40462,"MH...Da.":2900.19829,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":-0.36,"Deltam.z..Da.":-0.00035,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3232055.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":9.93028,"Ion.Inject.Time..ms.":22,"RT..min.":15.1472,"First.Scan":14870,"Last.Scan":14870,"Master.Scan.s.":14862,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_04.raw","File.ID":"F10","Quan.Info":"","Peptides.Matched":2428,"XCorr":6.19,"X..Protein.Groups":1,"Percolator.q.Value":0.0002,"Percolator.PEP":7.942e-13,"Percolator.SVMScore":4.966,"Precursor.Area":10036044,"Apex.RT..min.":15.09,"ptmRS..Binomial.Peptide.Score":622.31,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(13): 1413.58; b1+-H(3) O(4) P(14): 1528.61; b1+-H(3) O(4) P(16): 1771.69; y1+-H(3) O(4) P(15): 1750.78; y1+-H(3) O(4) P(14): 1621.73; b2+-H(3) O(4) P(16): 886.35; b2+-H(3) O(4) P(18): 1014.90; y2+-H(3) O(4) P(14): 811.37; b1+(12): 1344.56","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AA4)","Search.ID":"AA","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"GVFGYGFEEPSAIQQR","Annotated.Sequence":"[R].GVFGYGFEEPSAIQQR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6941,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":892.93428,"Contaminant":"False","Aligned.m.z..Da.":892.93461,"MH...Da.":1784.86129,"Theo..MH...Da.":1784.86022,"DeltaM..ppm.":0.6,"Deltam.z..Da.":0.00053,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":366840.84375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":20.2915,"Ion.Inject.Time..ms.":22,"RT..min.":15.0332,"First.Scan":14725,"Last.Scan":14725,"Master.Scan.s.":14719,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_04.raw","File.ID":"F10","Quan.Info":"","Peptides.Matched":1385,"XCorr":3.53,"X..Protein.Groups":1,"Percolator.q.Value":0.0002,"Percolator.PEP":6.531e-11,"Percolator.SVMScore":4.126,"Precursor.Area":310923.5625,"Apex.RT..min.":15.04,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AA4)","Search.ID":"AA","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEySDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); Y12(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.4022,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40608,"Contaminant":"False","Aligned.m.z..Da.":967.40644,"MH...Da.":2900.20371,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":1.51,"Deltam.z..Da.":0.00146,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":293923.71875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":15.0417,"First.Scan":14735,"Last.Scan":14735,"Master.Scan.s.":14732,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_04.raw","File.ID":"F10","Quan.Info":"","Peptides.Matched":2599,"XCorr":4.6,"X..Protein.Groups":1,"Percolator.q.Value":0.0002,"Percolator.PEP":9.957e-10,"Percolator.SVMScore":3.607,"Precursor.Area":10036044,"Apex.RT..min.":15.09,"ptmRS..Binomial.Peptide.Score":339.46,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(13): 1458.67; y2+-H(3) O(4) P(14): 811.37; b1+(12): 1344.56","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AA4)","Search.ID":"AA","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FTVSAIYSDLPQQER","Annotated.Sequence":"[K].FTVSAIYSDLPQQER.[D]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6854,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":877.44065,"Contaminant":"False","Aligned.m.z..Da.":877.44097,"MH...Da.":1753.87403,"Theo..MH...Da.":1753.87554,"DeltaM..ppm.":-0.86,"Deltam.z..Da.":-0.00076,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":381558.875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":15.0429,"First.Scan":14737,"Last.Scan":14737,"Master.Scan.s.":14732,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_04.raw","File.ID":"F10","Quan.Info":"","Peptides.Matched":1173,"XCorr":3.02,"X..Protein.Groups":1,"Percolator.q.Value":0.0002,"Percolator.PEP":2.074e-07,"Percolator.SVMScore":2.59,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AA4)","Search.ID":"AA","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0285,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40357,"Contaminant":"False","Aligned.m.z..Da.":967.4038,"MH...Da.":2900.19617,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":-1.09,"Deltam.z..Da.":-0.00105,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":213513.6875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":10.31126,"Ion.Inject.Time..ms.":22,"RT..min.":15.5499,"First.Scan":15359,"Last.Scan":15359,"Master.Scan.s.":15358,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_04.raw","File.ID":"F10","Quan.Info":"","Peptides.Matched":2331,"XCorr":4.21,"X..Protein.Groups":1,"Percolator.q.Value":0.0002,"Percolator.PEP":6.703e-08,"Percolator.SVMScore":2.805,"Precursor.Area":1998847.25,"Apex.RT..min.":15.37,"ptmRS..Binomial.Peptide.Score":309.28,"ptmRS..Isoform.Confidence.Probability":0.5,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"Y12(Phospho): 50; S13(Phospho): 50","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 50.0; S(13): 50.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AA4)","Search.ID":"AA","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].msEGITDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S2(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0912,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":731.31312,"Contaminant":"False","Aligned.m.z..Da.":731.31323,"MH...Da.":2191.92481,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":-1.38,"Deltam.z..Da.":-0.00101,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":203523.54688,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":15.7535,"First.Scan":15595,"Last.Scan":15595,"Master.Scan.s.":15587,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_04.raw","File.ID":"F10","Quan.Info":"","Peptides.Matched":1886,"XCorr":2.96,"X..Protein.Groups":1,"Percolator.q.Value":0.0002,"Percolator.PEP":0.00002,"Percolator.SVMScore":1.682,"Precursor.Area":684508.9375,"Apex.RT..min.":15.78,"ptmRS..Binomial.Peptide.Score":385.69,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(2): 112.04; b1+-H(3) O(4) P(3): 241.08; b1+-H(3) O(4) P(4): 298.10; b1+-H(3) O(4) P(5): 411.19; b1+-H(3) O(4) P(6): 512.24; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+-H(3) O(4) P(9): 869.39; b1+-H(3) O(4) P(10): 998.43","ptmRS..Best.Site.Probabilities":"S2(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 100.0; T(6): 0.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AA4)","Search.ID":"AA","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].msEGITDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S2(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.107,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1096.46919,"Contaminant":"False","Aligned.m.z..Da.":1096.46936,"MH...Da.":2191.93111,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":1.49,"Deltam.z..Da.":0.00163,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":226293.65625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":15.7335,"First.Scan":15570,"Last.Scan":15570,"Master.Scan.s.":15565,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_04.raw","File.ID":"F10","Quan.Info":"","Peptides.Matched":2052,"XCorr":3.83,"X..Protein.Groups":1,"Percolator.q.Value":0.0002,"Percolator.PEP":1.902e-10,"Percolator.SVMScore":3.923,"Precursor.Area":4131245.75,"Apex.RT..min.":15.78,"ptmRS..Binomial.Peptide.Score":508.27,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(2): 112.04; b1+-H(3) O(4) P(3): 241.08; b1+-H(3) O(4) P(4): 298.10; b1+-H(3) O(4) P(5): 411.19; b1+-H(3) O(4) P(6): 512.24; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+-H(3) O(4) P(9): 869.39; b1+-H(3) O(4) P(10): 998.43; b1+(4): 396.08; y1+(14): 1683.77","ptmRS..Best.Site.Probabilities":"S2(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 100.0; T(6): 0.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AA4)","Search.ID":"AA","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"AHNVSTSINSPSTDNDSISK","Annotated.Sequence":"[R].AHnVSTsInsPSTDNDSISK.[S]","Modifications":"N3(Deamidated); S7(Phospho); N9(Deamidated); S10(Phospho)","X..Proteins":2,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"Q12441; Q99231","Protein.Descriptions":"Transposon Ty1-DR3 Gag polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1A-DR3 PE=1 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.1333,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":745.9592,"Contaminant":"False","Aligned.m.z..Da.":745.95923,"MH...Da.":2235.86305,"Theo..MH...Da.":2235.86902,"DeltaM..ppm.":-2.67,"Deltam.z..Da.":-0.00199,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":958325,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":55.70444,"Ion.Inject.Time..ms.":22,"RT..min.":5.7987,"First.Scan":2638,"Last.Scan":2638,"Master.Scan.s.":2636,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_04.raw","File.ID":"F10","Quan.Info":"","Peptides.Matched":775,"XCorr":3,"X..Protein.Groups":1,"Percolator.q.Value":0.0002,"Percolator.PEP":0.00002,"Percolator.SVMScore":1.717,"ptmRS..Binomial.Peptide.Score":173.86,"ptmRS..Isoform.Confidence.Probability":0.017,"ptmRS..Isoform.Group.Report":"y2+(15): 823.84","ptmRS..Best.Site.Probabilities":"S5(Phospho): 96.6; S10(Phospho): 96.6","ptmRS..Phospho.Site.Probabilities":"S(5): 96.6; T(6): 3.4; S(7): 3.4; S(10): 96.6; S(12): 0.0; T(13): 0.0; S(17): 0.0; S(19): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AA4)","Search.ID":"AA","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].mSEGITDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5863,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1056.48414,"Contaminant":"False","Aligned.m.z..Da.":1056.48453,"MH...Da.":2111.96101,"Theo..MH...Da.":2111.96151,"DeltaM..ppm.":-0.24,"Deltam.z..Da.":-0.00025,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":439027.03125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":93.91922,"Ion.Inject.Time..ms.":22,"RT..min.":14.6693,"First.Scan":14298,"Last.Scan":14298,"Master.Scan.s.":14288,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_04.raw","File.ID":"F10","Quan.Info":"","Peptides.Matched":2254,"XCorr":2.78,"X..Protein.Groups":1,"Percolator.q.Value":0.0002,"Percolator.PEP":2.89e-10,"Percolator.SVMScore":3.843,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AA4)","Search.ID":"AA","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MFILDEADEMLSSGFK","Annotated.Sequence":"[K].mFILDEADEmLSSGFK.[E]","Modifications":"M1(Oxidation); M10(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.4372,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":932.92317,"Contaminant":"False","Aligned.m.z..Da.":932.9229,"MH...Da.":1864.83906,"Theo..MH...Da.":1864.83433,"DeltaM..ppm.":2.54,"Deltam.z..Da.":0.00236,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":209753.34375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":10.29717,"Ion.Inject.Time..ms.":22,"RT..min.":16.7104,"First.Scan":16709,"Last.Scan":16709,"Master.Scan.s.":16698,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_04.raw","File.ID":"F10","Quan.Info":"","Peptides.Matched":1870,"XCorr":1.83,"X..Protein.Groups":1,"Percolator.q.Value":0.0002,"Percolator.PEP":0.00002,"Percolator.SVMScore":1.735,"Precursor.Area":130460.63281,"Apex.RT..min.":16.69,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AA4)","Search.ID":"AA","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MGSINNNFNTNNNSNTDLDRDWK","Annotated.Sequence":"[-].mGSINNNFNTNNNsNTDLDRDWK.[T]","Modifications":"N-Term(Prot)(Met-loss); S14(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P39517","Master.Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","Protein.Accessions":"P39517","Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0653,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":878.36988,"Contaminant":"False","Aligned.m.z..Da.":878.37004,"MH...Da.":2633.0951,"Theo..MH...Da.":2633.10123,"DeltaM..ppm.":-2.33,"Deltam.z..Da.":-0.00204,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2235993,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":11.3253,"First.Scan":10011,"Last.Scan":10011,"Master.Scan.s.":10007,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_04.raw","File.ID":"F10","Quan.Info":"","Peptides.Matched":2131,"XCorr":3.98,"X..Protein.Groups":1,"Percolator.q.Value":0.0002,"Percolator.PEP":2.442e-10,"Percolator.SVMScore":3.875,"Precursor.Area":1375919.875,"Apex.RT..min.":11.32,"ptmRS..Binomial.Peptide.Score":500.03,"ptmRS..Isoform.Confidence.Probability":0.995,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(12): 1459.66; y1+-H(3) O(4) P(11): 1345.61; y1+-H(3) O(4) P(10): 1231.57; y2+-H(3) O(4) P(16): 968.43; y2+-H(3) O(4) P(15): 894.90; y2+-H(3) O(4) P(14): 837.88; y2+-H(3) O(4) P(13): 787.35; y2+-H(3) O(4) P(12): 730.33; y2+-H(3) O(4) P(11): 673.31; y2+-H(3) O(4) P(10): 616.29; y1+(8): 1048.51","ptmRS..Best.Site.Probabilities":"S14(Phospho): 99.49","ptmRS..Phospho.Site.Probabilities":"S(3): 0.0; T(10): 0.0; S(14): 99.5; T(16): 0.5"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AA4)","Search.ID":"AA","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"AIMPIIEGHDVLAQAQSGTGK","Annotated.Sequence":"[R].AImPIIEGHDVLAQAQSGTGK.[T]","Modifications":"M3(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.6847,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":718.04018,"Contaminant":"False","Aligned.m.z..Da.":718.04029,"MH...Da.":2152.10599,"Theo..MH...Da.":2152.10668,"DeltaM..ppm.":-0.32,"Deltam.z..Da.":-0.00023,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":358436.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":18.08374,"Ion.Inject.Time..ms.":22,"RT..min.":12.808,"First.Scan":12050,"Last.Scan":12050,"Master.Scan.s.":12048,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_04.raw","File.ID":"F10","Quan.Info":"","Peptides.Matched":1019,"XCorr":3.33,"X..Protein.Groups":1,"Percolator.q.Value":0.0002,"Percolator.PEP":1.974e-09,"Percolator.SVMScore":3.477,"Precursor.Area":604284,"Apex.RT..min.":12.8,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"RLAFVHLVEPR","Annotated.Sequence":"[K].RLAFVHLVEPR.[V]","Modifications":"","X..Proteins":2,"Master.Protein.Accessions":"Q03558","Master.Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","Protein.Accessions":"Q03558; P41816","Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3; NADPH dehydrogenase 3 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE3 PE=1 SV=2","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.6028,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":446.26709,"Contaminant":"False","Aligned.m.z..Da.":446.26709,"MH...Da.":1336.78671,"Theo..MH...Da.":1336.78482,"DeltaM..ppm.":1.42,"Deltam.z..Da.":0.00063,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1901124.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":28.55417,"Ion.Inject.Time..ms.":22,"RT..min.":12.0698,"First.Scan":11206,"Last.Scan":11206,"Master.Scan.s.":11195,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":74,"XCorr":2.82,"X..Protein.Groups":1,"Percolator.q.Value":0.00021,"Percolator.PEP":0.00204,"Percolator.SVMScore":0.823,"Precursor.Area":1280266.875,"Apex.RT..min.":12.07,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LQQTAFDKK","Annotated.Sequence":"[R].LQQTAFDKK.[S]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5281,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":539.79962,"Contaminant":"False","Aligned.m.z..Da.":539.79962,"MH...Da.":1078.59195,"Theo..MH...Da.":1078.58914,"DeltaM..ppm.":2.61,"Deltam.z..Da.":0.00141,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":12065993,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":21.36282,"Ion.Inject.Time..ms.":7.686,"RT..min.":5.5273,"First.Scan":2631,"Last.Scan":2631,"Master.Scan.s.":2628,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":384,"XCorr":2.31,"X..Protein.Groups":1,"Percolator.q.Value":0.00021,"Percolator.PEP":0.00185,"Percolator.SVMScore":0.841,"Precursor.Area":16370569,"Apex.RT..min.":5.54,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"HSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[R].HSDSYsENETNHTNVPISSTGGTNNK.[T]","Modifications":"S6(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":0,"Charge":4,"Original.Precursor.Charge":2,"DeltaScore":0.019,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":718.29964,"Contaminant":"False","Aligned.m.z..Da.":718.29964,"MH...Da.":2870.17672,"Theo..MH...Da.":2870.18608,"DeltaM..ppm.":-3.26,"Deltam.z..Da.":-0.00234,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":4349808,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":64.22885,"Ion.Inject.Time..ms.":22,"RT..min.":7.6162,"First.Scan":5379,"Last.Scan":5379,"Master.Scan.s.":5371,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":2206,"XCorr":1.58,"X..Protein.Groups":1,"Percolator.q.Value":0.00021,"Percolator.PEP":0.00159,"Percolator.SVMScore":0.868,"Precursor.Area":10104933,"Apex.RT..min.":7.59,"ptmRS..Binomial.Peptide.Score":93.93,"ptmRS..Isoform.Confidence.Probability":0.2,"ptmRS..Isoform.Group.Report":"b2+-H(3) O(4) P(14): 799.81; b2+-H(3) O(4) P(15): 849.34","ptmRS..Best.Site.Probabilities":"S2(Phospho): 20; S4(Phospho): 20; S6(Phospho): 20; T10(Phospho): 20; T13(Phospho): 20","ptmRS..Phospho.Site.Probabilities":"S(2): 20.0; S(4): 20.0; Y(5): 0.0; S(6): 20.0; T(10): 20.0; T(13): 20.0; S(18): 0.0; S(19): 0.0; T(20): 0.0; T(23): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"NHQLEEEIEK","Annotated.Sequence":"[K].NHQLEEEIEK.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.4413,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":423.54167,"Contaminant":"False","Aligned.m.z..Da.":423.54167,"MH...Da.":1268.61046,"Theo..MH...Da.":1268.61172,"DeltaM..ppm.":-0.99,"Deltam.z..Da.":-0.00042,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3346546,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":68.71042,"Ion.Inject.Time..ms.":9.652,"RT..min.":7.1913,"First.Scan":4821,"Last.Scan":4821,"Master.Scan.s.":4812,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":928,"XCorr":2.47,"X..Protein.Groups":1,"Percolator.q.Value":0.00021,"Percolator.PEP":0.00265,"Percolator.SVMScore":0.775,"Precursor.Area":4592155.5,"Apex.RT..min.":7.2,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"YDRDTFYK","Annotated.Sequence":"[K].YDRDTFYK.[M]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q03558","Master.Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","Protein.Accessions":"Q03558","Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5537,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":554.25895,"Contaminant":"False","Aligned.m.z..Da.":554.25895,"MH...Da.":1107.51062,"Theo..MH...Da.":1107.51055,"DeltaM..ppm.":0.06,"Deltam.z..Da.":0.00003,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":4005672,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":6.946,"Ion.Inject.Time..ms.":22,"RT..min.":7.3458,"First.Scan":5023,"Last.Scan":5023,"Master.Scan.s.":5020,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":557,"XCorr":2.42,"X..Protein.Groups":1,"Percolator.q.Value":0.00021,"Percolator.PEP":0.00164,"Percolator.SVMScore":0.863,"Precursor.Area":2767058.5,"Apex.RT..min.":7.35,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (X4)","Search.ID":"X","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MGSINNNFNTNNNSNTDLDRDWK","Annotated.Sequence":"[-].mGSINNNFNTNNNsNTDLDRDWK.[T]","Modifications":"N-Term(Prot)(Met-loss); S14(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P39517","Master.Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","Protein.Accessions":"P39517","Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0525,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":878.37205,"Contaminant":"False","Aligned.m.z..Da.":878.37205,"MH...Da.":2633.10161,"Theo..MH...Da.":2633.10123,"DeltaM..ppm.":0.14,"Deltam.z..Da.":0.00013,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":499644.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":11.287,"First.Scan":9879,"Last.Scan":9879,"Master.Scan.s.":9875,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_01.raw","File.ID":"F13","Quan.Info":"","Peptides.Matched":2184,"XCorr":3.81,"X..Protein.Groups":1,"Percolator.q.Value":0.00021,"Percolator.PEP":6.415e-10,"Percolator.SVMScore":4.139,"Precursor.Area":2671132.75,"Apex.RT..min.":11.31,"ptmRS..Binomial.Peptide.Score":422.08,"ptmRS..Isoform.Confidence.Probability":0.995,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(11): 1345.61; y1+-H(3) O(4) P(10): 1231.57; y2+-H(3) O(4) P(19): 1139.50; y2+-H(3) O(4) P(18): 1082.48; y2+-H(3) O(4) P(17): 1025.45; y2+-H(3) O(4) P(16): 968.43; y2+-H(3) O(4) P(15): 894.90; y2+-H(3) O(4) P(14): 837.88; y2+-H(3) O(4) P(12): 730.33; y2+-H(3) O(4) P(11): 673.31; y2+-H(3) O(4) P(10): 616.29; y1+(9): 1162.55","ptmRS..Best.Site.Probabilities":"S14(Phospho): 99.45","ptmRS..Phospho.Site.Probabilities":"S(3): 0.0; T(10): 0.0; S(14): 99.5; T(16): 0.6"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (X4)","Search.ID":"X","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"APQALMLAPTR","Annotated.Sequence":"[K].APQALMLAPTR.[E]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5843,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":584.82896,"Contaminant":"False","Aligned.m.z..Da.":584.82895,"MH...Da.":1168.65065,"Theo..MH...Da.":1168.65069,"DeltaM..ppm.":-0.03,"Deltam.z..Da.":-0.00002,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":676739.875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":78.41093,"Ion.Inject.Time..ms.":22,"RT..min.":11.6248,"First.Scan":10337,"Last.Scan":10337,"Master.Scan.s.":10330,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_01.raw","File.ID":"F13","Quan.Info":"","Peptides.Matched":162,"XCorr":2.55,"X..Protein.Groups":1,"Percolator.q.Value":0.00021,"Percolator.PEP":1.715e-06,"Percolator.SVMScore":2.464,"Precursor.Area":560648.25,"Apex.RT..min.":11.63,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (X4)","Search.ID":"X","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KDELTLEGIK","Annotated.Sequence":"[K].KDELTLEGIK.[Q]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.4589,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":573.32401,"Contaminant":"False","Aligned.m.z..Da.":573.32399,"MH...Da.":1145.64074,"Theo..MH...Da.":1145.64123,"DeltaM..ppm.":-0.43,"Deltam.z..Da.":-0.00024,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":974903.375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":24.04841,"Ion.Inject.Time..ms.":22,"RT..min.":10.2355,"First.Scan":8532,"Last.Scan":8532,"Master.Scan.s.":8523,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_01.raw","File.ID":"F13","Quan.Info":"","Peptides.Matched":314,"XCorr":3.53,"X..Protein.Groups":1,"Percolator.q.Value":0.00021,"Percolator.PEP":5.149e-07,"Percolator.SVMScore":2.719,"Precursor.Area":1359598.875,"Apex.RT..min.":10.25,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (X4)","Search.ID":"X","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"APQALMLAPTR","Annotated.Sequence":"[K].APQALmLAPTR.[E]","Modifications":"M6(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":592.82621,"Contaminant":"False","Aligned.m.z..Da.":592.82619,"MH...Da.":1184.64514,"Theo..MH...Da.":1184.6456,"DeltaM..ppm.":-0.39,"Deltam.z..Da.":-0.00023,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1796962.875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":8.27226,"Ion.Inject.Time..ms.":22,"RT..min.":10.1805,"First.Scan":8463,"Last.Scan":8463,"Master.Scan.s.":8458,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_01.raw","File.ID":"F13","Quan.Info":"","Peptides.Matched":325,"XCorr":2.7,"X..Protein.Groups":1,"Percolator.q.Value":0.00021,"Percolator.PEP":0.00003,"Percolator.SVMScore":1.89,"Precursor.Area":1465376.25,"Apex.RT..min.":10.19,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (X4)","Search.ID":"X","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"TGTFSIAALQR","Annotated.Sequence":"[K].TGTFSIAALQR.[I]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7781,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":582.82219,"Contaminant":"False","Aligned.m.z..Da.":582.82227,"MH...Da.":1164.6371,"Theo..MH...Da.":1164.63715,"DeltaM..ppm.":-0.04,"Deltam.z..Da.":-0.00002,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":237334.6875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":17.86536,"Ion.Inject.Time..ms.":22,"RT..min.":13.3639,"First.Scan":12636,"Last.Scan":12636,"Master.Scan.s.":12627,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_01.raw","File.ID":"F13","Quan.Info":"","Peptides.Matched":361,"XCorr":3.11,"X..Protein.Groups":1,"Percolator.q.Value":0.00021,"Percolator.PEP":6.342e-06,"Percolator.SVMScore":2.186,"Precursor.Area":414428.15625,"Apex.RT..min.":13.38,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (X4)","Search.ID":"X","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"AIMPIIEGHDVLAQAQSGTGK","Annotated.Sequence":"[R].AImPIIEGHDVLAQAQSGTGK.[T]","Modifications":"M3(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.6774,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":718.04211,"Contaminant":"False","Aligned.m.z..Da.":718.04213,"MH...Da.":2152.1118,"Theo..MH...Da.":2152.10668,"DeltaM..ppm.":2.38,"Deltam.z..Da.":0.00171,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":365260.96875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":5.16378,"Ion.Inject.Time..ms.":22,"RT..min.":12.7373,"First.Scan":11846,"Last.Scan":11846,"Master.Scan.s.":11838,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_01.raw","File.ID":"F13","Quan.Info":"","Peptides.Matched":979,"XCorr":3.72,"X..Protein.Groups":1,"Percolator.q.Value":0.00021,"Percolator.PEP":4.352e-13,"Percolator.SVMScore":5.689,"Precursor.Area":506871.34375,"Apex.RT..min.":12.76,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (X4)","Search.ID":"X","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].msEGITDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S2(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.1088,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":731.31587,"Contaminant":"False","Aligned.m.z..Da.":731.31581,"MH...Da.":2191.93306,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":2.38,"Deltam.z..Da.":0.00174,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":218601.32812,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":6.97425,"Ion.Inject.Time..ms.":22,"RT..min.":15.7664,"First.Scan":15261,"Last.Scan":15261,"Master.Scan.s.":15251,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_01.raw","File.ID":"F13","Quan.Info":"","Peptides.Matched":2177,"XCorr":3.4,"X..Protein.Groups":1,"Percolator.q.Value":0.00021,"Percolator.PEP":8.774e-06,"Percolator.SVMScore":2.117,"ptmRS..Binomial.Peptide.Score":405,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(2): 112.04; b1+-H(3) O(4) P(3): 241.08; b1+-H(3) O(4) P(4): 298.10; b1+-H(3) O(4) P(5): 411.19; b1+-H(3) O(4) P(6): 512.24; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+-H(3) O(4) P(9): 869.39; b1+-H(3) O(4) P(10): 998.43; b2+-H(3) O(4) P(9): 435.20","ptmRS..Best.Site.Probabilities":"S2(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 100.0; T(6): 0.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (X4)","Search.ID":"X","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].msEGITDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S2(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":3,"DeltaScore":0.1914,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1096.46856,"Contaminant":"False","Aligned.m.z..Da.":1096.46847,"MH...Da.":2191.92983,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":0.91,"Deltam.z..Da.":0.00099,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":168918.82812,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":71.32434,"Ion.Inject.Time..ms.":22,"RT..min.":15.7439,"First.Scan":15233,"Last.Scan":15233,"Master.Scan.s.":15232,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_01.raw","File.ID":"F13","Quan.Info":"","Peptides.Matched":2028,"XCorr":2.56,"X..Protein.Groups":1,"Percolator.q.Value":0.00021,"Percolator.PEP":9.484e-06,"Percolator.SVMScore":2.1,"Precursor.Area":7025261,"Apex.RT..min.":15.8,"ptmRS..Binomial.Peptide.Score":302.59,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(2): 112.04; b1+-H(3) O(4) P(3): 241.08; b1+-H(3) O(4) P(4): 298.10; b1+-H(3) O(4) P(5): 411.19; b1+-H(3) O(4) P(6): 512.24; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+-H(3) O(4) P(9): 869.39","ptmRS..Best.Site.Probabilities":"S2(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 100.0; T(6): 0.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (X4)","Search.ID":"X","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0481,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40513,"Contaminant":"False","Aligned.m.z..Da.":967.40509,"MH...Da.":2900.20086,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":0.53,"Deltam.z..Da.":0.00051,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":361981.125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":3.82989,"Ion.Inject.Time..ms.":22,"RT..min.":15.5356,"First.Scan":15011,"Last.Scan":15011,"Master.Scan.s.":15010,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_01.raw","File.ID":"F13","Quan.Info":"","Peptides.Matched":2511,"XCorr":5.2,"X..Protein.Groups":1,"Percolator.q.Value":0.00021,"Percolator.PEP":9.708e-09,"Percolator.SVMScore":3.562,"Precursor.Area":8301051,"Apex.RT..min.":15.09,"ptmRS..Binomial.Peptide.Score":398.88,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(14): 1621.73; b1+(12): 1344.56","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (X4)","Search.ID":"X","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DFRHSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[K].DFRHsDsYSENETNHTNVPISSTGGTNNK.[T]","Modifications":"S5(Phospho); S7(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":1,"Charge":4,"Original.Precursor.Charge":4,"DeltaScore":0.0221,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":842.84433,"Contaminant":"False","Aligned.m.z..Da.":842.84442,"MH...Da.":3368.3555,"Theo..MH...Da.":3368.34888,"DeltaM..ppm.":1.97,"Deltam.z..Da.":0.00166,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":999781.0625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":8.6642,"First.Scan":6665,"Last.Scan":6665,"Master.Scan.s.":6664,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_01.raw","File.ID":"F13","Quan.Info":"","Peptides.Matched":1148,"XCorr":4.97,"X..Protein.Groups":1,"Percolator.q.Value":0.00021,"Percolator.PEP":3.528e-07,"Percolator.SVMScore":2.799,"ptmRS..Binomial.Peptide.Score":294.8,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b2+-H(3) O(4) P(7): 454.16; b2+-H(3) O(4) P(8): 535.69; b2+- -2H(3) O(4) P -2H(3) O(4) P(18): 1049.44; b2+- -2H(3) O(4) P -2H(3) O(4) P(20): 1154.50","ptmRS..Best.Site.Probabilities":"S5(Phospho): 100; S7(Phospho): 99.96","ptmRS..Phospho.Site.Probabilities":"S(5): 100.0; S(7): 100.0; Y(8): 0.0; S(9): 0.0; T(13): 0.0; T(16): 0.0; S(21): 0.0; S(22): 0.0; T(23): 0.0; T(26): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (X4)","Search.ID":"X","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FDDMELDENLLR","Annotated.Sequence":"[K].FDDMELDENLLR.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.4316,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":755.34686,"Contaminant":"False","Aligned.m.z..Da.":755.34678,"MH...Da.":1509.68645,"Theo..MH...Da.":1509.68898,"DeltaM..ppm.":-1.68,"Deltam.z..Da.":-0.00127,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":390029.78125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":68.70753,"Ion.Inject.Time..ms.":22,"RT..min.":16.0433,"First.Scan":15581,"Last.Scan":15581,"Master.Scan.s.":15574,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_01.raw","File.ID":"F13","Quan.Info":"","Peptides.Matched":1007,"XCorr":2.34,"X..Protein.Groups":1,"Percolator.q.Value":0.00021,"Percolator.PEP":5.425e-06,"Percolator.SVMScore":2.219,"Precursor.Area":392956.5,"Apex.RT..min.":16.05,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (X4)","Search.ID":"X","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":5,"DeltaScore":0.0896,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40466,"Contaminant":"False","Aligned.m.z..Da.":967.40473,"MH...Da.":2900.19942,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":0.03,"Deltam.z..Da.":0.00003,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":6619831,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":2.82478,"Ion.Inject.Time..ms.":22,"RT..min.":15.1094,"First.Scan":14526,"Last.Scan":14526,"Master.Scan.s.":14521,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_01.raw","File.ID":"F13","Quan.Info":"","Peptides.Matched":2487,"XCorr":7.37,"X..Protein.Groups":1,"Percolator.q.Value":0.00021,"Percolator.PEP":2.486e-16,"Percolator.SVMScore":8.483,"Precursor.Area":8301051,"Apex.RT..min.":15.09,"ptmRS..Binomial.Peptide.Score":776.7,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(13): 1413.58; b1+-H(3) O(4) P(14): 1528.61; b1+-H(3) O(4) P(15): 1642.65; b1+-H(3) O(4) P(16): 1771.69; b1+-H(3) O(4) P(18): 2028.79; y1+-H(3) O(4) P(15): 1750.78; y1+-H(3) O(4) P(14): 1621.73; y1+-H(3) O(4) P(13): 1458.67; b2+-H(3) O(4) P(16): 886.35; b2+-H(3) O(4) P(18): 1014.90; y2+-H(3) O(4) P(14): 811.37; b1+(12): 1344.56; y1+(13): 1556.65","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (X4)","Search.ID":"X","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FTVSAIYSDLPQQER","Annotated.Sequence":"[K].FTVSAIYSDLPQQER.[D]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6872,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":877.44424,"Contaminant":"False","Aligned.m.z..Da.":877.44433,"MH...Da.":1753.8812,"Theo..MH...Da.":1753.87554,"DeltaM..ppm.":3.23,"Deltam.z..Da.":0.00283,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":378057.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":36.24354,"Ion.Inject.Time..ms.":22,"RT..min.":15.0055,"First.Scan":14397,"Last.Scan":14397,"Master.Scan.s.":14391,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_01.raw","File.ID":"F13","Quan.Info":"","Peptides.Matched":1175,"XCorr":3.58,"X..Protein.Groups":1,"Percolator.q.Value":0.00021,"Percolator.PEP":1.242e-08,"Percolator.SVMScore":3.51,"Precursor.Area":405276.71875,"Apex.RT..min.":15.01,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (X4)","Search.ID":"X","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"GVFGYGFEEPSAIQQR","Annotated.Sequence":"[R].GVFGYGFEEPSAIQQR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.613,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":892.93342,"Contaminant":"False","Aligned.m.z..Da.":892.93352,"MH...Da.":1784.85956,"Theo..MH...Da.":1784.86022,"DeltaM..ppm.":-0.37,"Deltam.z..Da.":-0.00033,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":400418.84375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":6.6167,"Ion.Inject.Time..ms.":22,"RT..min.":15.0049,"First.Scan":14396,"Last.Scan":14396,"Master.Scan.s.":14391,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_01.raw","File.ID":"F13","Quan.Info":"","Peptides.Matched":1300,"XCorr":3.23,"X..Protein.Groups":1,"Percolator.q.Value":0.00021,"Percolator.PEP":5.739e-08,"Percolator.SVMScore":3.185,"Precursor.Area":581383.5625,"Apex.RT..min.":15.02,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (X4)","Search.ID":"X","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":4,"DeltaScore":0.0027,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40489,"Contaminant":"False","Aligned.m.z..Da.":967.40488,"MH...Da.":2900.20013,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":0.28,"Deltam.z..Da.":0.00027,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":855222.4375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":47.67994,"Ion.Inject.Time..ms.":22,"RT..min.":15.4307,"First.Scan":14901,"Last.Scan":14901,"Master.Scan.s.":14892,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_01.raw","File.ID":"F13","Quan.Info":"","Peptides.Matched":2493,"XCorr":3.77,"X..Protein.Groups":1,"Percolator.q.Value":0.00021,"Percolator.PEP":4.464e-07,"Percolator.SVMScore":2.749,"Precursor.Area":1814811.125,"Apex.RT..min.":15.38,"ptmRS..Binomial.Peptide.Score":286,"ptmRS..Isoform.Confidence.Probability":0.5,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"Y12(Phospho): 50; S13(Phospho): 50","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 50.0; S(13): 50.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (X4)","Search.ID":"X","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":5,"DeltaScore":0.0614,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40441,"Contaminant":"False","Aligned.m.z..Da.":967.40444,"MH...Da.":2900.19867,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":-0.23,"Deltam.z..Da.":-0.00022,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":926203.375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":39.38094,"Ion.Inject.Time..ms.":22,"RT..min.":15.251,"First.Scan":14683,"Last.Scan":14683,"Master.Scan.s.":14681,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_01.raw","File.ID":"F13","Quan.Info":"","Peptides.Matched":2458,"XCorr":5.7,"X..Protein.Groups":1,"Percolator.q.Value":0.00021,"Percolator.PEP":1.925e-13,"Percolator.SVMScore":5.862,"Precursor.Area":8301051,"Apex.RT..min.":15.09,"ptmRS..Binomial.Peptide.Score":567.57,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(13): 1413.58; b1+-H(3) O(4) P(14): 1528.61; y1+-H(3) O(4) P(14): 1621.73; y1+-H(3) O(4) P(13): 1458.67; b2+-H(3) O(4) P(16): 886.35; b2+-H(3) O(4) P(18): 1014.90; y2+-H(3) O(4) P(14): 811.37; b1+(12): 1344.56","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (X4)","Search.ID":"X","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FFISNPDLVDRLEK","Annotated.Sequence":"[R].FFISNPDLVDRLEK.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q03558","Master.Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","Protein.Accessions":"Q03558","Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.6328,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":564.96943,"Contaminant":"False","Aligned.m.z..Da.":564.96944,"MH...Da.":1692.89375,"Theo..MH...Da.":1692.89555,"DeltaM..ppm.":-1.06,"Deltam.z..Da.":-0.0006,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":268017.84375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":54.98687,"Ion.Inject.Time..ms.":22,"RT..min.":15.3054,"First.Scan":14747,"Last.Scan":14747,"Master.Scan.s.":14736,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_01.raw","File.ID":"F13","Quan.Info":"","Peptides.Matched":535,"XCorr":1.77,"X..Protein.Groups":1,"Percolator.q.Value":0.00021,"Percolator.PEP":7.343e-06,"Percolator.SVMScore":2.155,"Precursor.Area":182005.125,"Apex.RT..min.":15.31,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (X4)","Search.ID":"X","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].mSEGITDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7008,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1056.48565,"Contaminant":"False","Aligned.m.z..Da.":1056.48588,"MH...Da.":2111.96403,"Theo..MH...Da.":2111.96151,"DeltaM..ppm.":1.19,"Deltam.z..Da.":0.00126,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":289063.5625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":93.91547,"Ion.Inject.Time..ms.":22,"RT..min.":14.6495,"First.Scan":14016,"Last.Scan":14016,"Master.Scan.s.":14011,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_01.raw","File.ID":"F13","Quan.Info":"","Peptides.Matched":2349,"XCorr":3.81,"X..Protein.Groups":1,"Percolator.q.Value":0.00021,"Percolator.PEP":8.171e-10,"Percolator.SVMScore":4.088,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (M4)","Search.ID":"M","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSSPLHRLSPTGK","Annotated.Sequence":"[R].mSsPLHRLsPTGK.[S]","Modifications":"M1(Oxidation); S3(Phospho); S9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":793.84356,"Contaminant":"False","Aligned.m.z..Da.":793.84361,"MH...Da.":1586.67984,"Theo..MH...Da.":1586.67977,"DeltaM..ppm.":0.05,"Deltam.z..Da.":0.00004,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2510567.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":10.44735,"Ion.Inject.Time..ms.":22,"RT..min.":7.7044,"First.Scan":5827,"Last.Scan":5827,"Master.Scan.s.":5817,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_06.raw","File.ID":"F24","Quan.Info":"","Peptides.Matched":975,"XCorr":2.72,"X..Protein.Groups":1,"Percolator.q.Value":0.00021,"Percolator.PEP":0.00003,"Percolator.SVMScore":1.719,"Precursor.Area":2232934.5,"Apex.RT..min.":7.7,"ptmRS..Binomial.Peptide.Score":364.72,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(8): 877.50; y1+-H(3) O(4) P(5): 471.26; y2+-H(3) O(4) P(11): 627.82; y2+-H(3) O(4) P(10): 544.32; y2+-H(3) O(4) P(8): 439.25; b1+- -2H(3) O(4) P -2H(3) O(4) P(9): 989.50; y1+(4): 402.23; y1+(3): 305.18","ptmRS..Best.Site.Probabilities":"S3(Phospho): 100; S9(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(3): 100.0; S(9): 100.0; T(11): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (M4)","Search.ID":"M","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"IQDPQMTGYVSTR","Annotated.Sequence":"[R].IQDPQmTGyVSTR.[Y]","Modifications":"M6(Oxidation); Y9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P32485","Master.Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","Protein.Accessions":"P32485","Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1284,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":796.34591,"Contaminant":"False","Aligned.m.z..Da.":796.34596,"MH...Da.":1591.68455,"Theo..MH...Da.":1591.6822,"DeltaM..ppm.":1.48,"Deltam.z..Da.":0.00118,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2022683.125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":8.53758,"Ion.Inject.Time..ms.":22,"RT..min.":7.5881,"First.Scan":5666,"Last.Scan":5666,"Master.Scan.s.":5661,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_06.raw","File.ID":"F24","Quan.Info":"","Peptides.Matched":1113,"XCorr":2.96,"X..Protein.Groups":1,"Percolator.q.Value":0.00021,"Percolator.PEP":3.607e-06,"Percolator.SVMScore":2.115,"Precursor.Area":7056280,"Apex.RT..min.":7.63,"ptmRS..Binomial.Peptide.Score":274.68,"ptmRS..Isoform.Confidence.Probability":0.992,"ptmRS..Isoform.Group.Report":"y1+(6): 762.32; y1+(5): 705.30","ptmRS..Best.Site.Probabilities":"Y9(Phospho): 99.15","ptmRS..Phospho.Site.Probabilities":"T(7): 0.4; Y(9): 99.2; S(11): 0.4; T(12): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (M4)","Search.ID":"M","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"HSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[R].HSDSYsENETNHTNVPISSTGGTNNK.[T]","Modifications":"S6(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0135,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":957.39908,"Contaminant":"False","Aligned.m.z..Da.":957.39913,"MH...Da.":2870.1827,"Theo..MH...Da.":2870.18608,"DeltaM..ppm.":-1.18,"Deltam.z..Da.":-0.00113,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3026524.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":7.4941,"First.Scan":5536,"Last.Scan":5536,"Master.Scan.s.":5531,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_06.raw","File.ID":"F24","Quan.Info":"","Peptides.Matched":2353,"XCorr":6.66,"X..Protein.Groups":1,"Percolator.q.Value":0.00021,"Percolator.PEP":1.274e-13,"Percolator.SVMScore":5.466,"Precursor.Area":11324681,"Apex.RT..min.":7.51,"ptmRS..Binomial.Peptide.Score":522.71,"ptmRS..Isoform.Confidence.Probability":0.498,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(11): 1246.46; b1+-H(3) O(4) P(12): 1383.52; b1+-H(3) O(4) P(15): 1697.68; b2+-H(3) O(4) P(12): 692.26; b2+-H(3) O(4) P(13): 742.79; b2+-H(3) O(4) P(14): 799.81; b2+-H(3) O(4) P(15): 849.34; b2+-H(3) O(4) P(17): 954.41; y2+-H(3) O(4) P(25): 1318.08; y2+-H(3) O(4) P(23): 1217.05; y2+-H(3) O(4) P(21): 1092.00","ptmRS..Best.Site.Probabilities":"S6(Phospho): 49.84; T10(Phospho): 49.84","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(4): 0.3; Y(5): 0.0; S(6): 49.8; T(10): 49.8; T(13): 0.0; S(18): 0.0; S(19): 0.0; T(20): 0.0; T(23): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (M4)","Search.ID":"M","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"AAEAGETGAATSATEGDNNNNTAAGDKK","Annotated.Sequence":"[K].AAEAGETGAATsATEGDNNNNTAAGDKK.[G]","Modifications":"S12(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0294,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":906.05014,"Contaminant":"False","Aligned.m.z..Da.":906.05004,"MH...Da.":2716.13586,"Theo..MH...Da.":2716.13298,"DeltaM..ppm.":1.06,"Deltam.z..Da.":0.00096,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":4280107.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":4.78188,"Ion.Inject.Time..ms.":22,"RT..min.":5.0958,"First.Scan":2120,"Last.Scan":2120,"Master.Scan.s.":2112,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_06.raw","File.ID":"F24","Quan.Info":"","Peptides.Matched":2506,"XCorr":6.46,"X..Protein.Groups":1,"Percolator.q.Value":0.00021,"Percolator.PEP":1.347e-15,"Percolator.SVMScore":6.671,"ptmRS..Binomial.Peptide.Score":503.12,"ptmRS..Isoform.Confidence.Probability":0.992,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(18): 1789.79; y1+-H(3) O(4) P(17): 1688.75; y2+-H(3) O(4) P(25): 1174.02; y2+-H(3) O(4) P(24): 1138.50; y2+-H(3) O(4) P(22): 1045.47; y2+-H(3) O(4) P(18): 895.40; y2+-H(3) O(4) P(17): 844.88","ptmRS..Best.Site.Probabilities":"S12(Phospho): 99.23","ptmRS..Phospho.Site.Probabilities":"T(7): 0.8; T(11): 0.0; S(12): 99.2; T(14): 0.0; T(22): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (X4)","Search.ID":"X","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"AAEAGETGAATSATEGDNNNNTAAGDKK","Annotated.Sequence":"[K].AAEAGETGAATsATEGDNNNNTAAGDKK.[G]","Modifications":"S12(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0236,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":906.04948,"Contaminant":"False","Aligned.m.z..Da.":906.04949,"MH...Da.":2716.13388,"Theo..MH...Da.":2716.13298,"DeltaM..ppm.":0.33,"Deltam.z..Da.":0.0003,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3815897.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":5.0306,"First.Scan":1767,"Last.Scan":1767,"Master.Scan.s.":1763,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_01.raw","File.ID":"F13","Quan.Info":"","Peptides.Matched":2558,"XCorr":7.21,"X..Protein.Groups":1,"Percolator.q.Value":0.00021,"Percolator.PEP":1.601e-15,"Percolator.SVMScore":7.214,"ptmRS..Binomial.Peptide.Score":508.19,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(15): 1300.57; y1+-H(3) O(4) P(18): 1789.79; y1+-H(3) O(4) P(17): 1688.75; y2+-H(3) O(4) P(21): 994.95; y2+-H(3) O(4) P(18): 895.40; y2+-H(3) O(4) P(17): 844.88","ptmRS..Best.Site.Probabilities":"S12(Phospho): 99.99","ptmRS..Phospho.Site.Probabilities":"T(7): 0.0; T(11): 0.0; S(12): 100.0; T(14): 0.0; T(22): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (X4)","Search.ID":"X","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DIFSNDELLSDAYDAK","Annotated.Sequence":"[K].DIFSNDELLSDAYDAK.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7216,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":908.42087,"Contaminant":"False","Aligned.m.z..Da.":908.42076,"MH...Da.":1815.83448,"Theo..MH...Da.":1815.82831,"DeltaM..ppm.":3.39,"Deltam.z..Da.":0.00308,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":363368.15625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":11.39845,"Ion.Inject.Time..ms.":22,"RT..min.":17.0557,"First.Scan":16693,"Last.Scan":16693,"Master.Scan.s.":16689,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_01.raw","File.ID":"F13","Quan.Info":"","Peptides.Matched":1826,"XCorr":3.7,"X..Protein.Groups":1,"Percolator.q.Value":0.00021,"Percolator.PEP":3.56e-09,"Percolator.SVMScore":3.775,"Precursor.Area":1442239.75,"Apex.RT..min.":17.08,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (X4)","Search.ID":"X","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0036,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.4042,"Contaminant":"False","Aligned.m.z..Da.":967.40404,"MH...Da.":2900.19806,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":-0.44,"Deltam.z..Da.":-0.00042,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":123250.70312,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":35.3271,"Ion.Inject.Time..ms.":22,"RT..min.":17.3945,"First.Scan":17095,"Last.Scan":17095,"Master.Scan.s.":17094,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_01.raw","File.ID":"F13","Quan.Info":"","Peptides.Matched":2439,"XCorr":2.78,"X..Protein.Groups":1,"Percolator.q.Value":0.00021,"Percolator.PEP":7.207e-06,"Percolator.SVMScore":2.159,"ptmRS..Binomial.Peptide.Score":282.08,"ptmRS..Isoform.Confidence.Probability":0.5,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"Y12(Phospho): 50; S13(Phospho): 50","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 50.0; S(13): 50.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (X4)","Search.ID":"X","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.4045,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40538,"Contaminant":"False","Aligned.m.z..Da.":967.40527,"MH...Da.":2900.20159,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":0.78,"Deltam.z..Da.":0.00076,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":93857.26562,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":5.5805,"Ion.Inject.Time..ms.":22,"RT..min.":16.5697,"First.Scan":16144,"Last.Scan":16144,"Master.Scan.s.":16142,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_01.raw","File.ID":"F13","Quan.Info":"","Peptides.Matched":2522,"XCorr":3.56,"X..Protein.Groups":1,"Percolator.q.Value":0.00021,"Percolator.PEP":1.817e-07,"Percolator.SVMScore":2.94,"ptmRS..Binomial.Peptide.Score":328.86,"ptmRS..Isoform.Confidence.Probability":0.993,"ptmRS..Isoform.Group.Report":"y2+-H(3) O(4) P(14): 811.37","ptmRS..Best.Site.Probabilities":"S13(Phospho): 99.28","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.7; S(13): 99.3; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (X4)","Search.ID":"X","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MFILDEADEMLSSGFK","Annotated.Sequence":"[K].mFILDEADEmLSSGFK.[E]","Modifications":"M1(Oxidation); M10(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.668,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":932.91901,"Contaminant":"False","Aligned.m.z..Da.":932.9189,"MH...Da.":1864.83074,"Theo..MH...Da.":1864.83433,"DeltaM..ppm.":-1.92,"Deltam.z..Da.":-0.00179,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":430733.09375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":16.6914,"First.Scan":16274,"Last.Scan":16274,"Master.Scan.s.":16268,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_01.raw","File.ID":"F13","Quan.Info":"","Peptides.Matched":1833,"XCorr":2.41,"X..Protein.Groups":1,"Percolator.q.Value":0.00021,"Percolator.PEP":1.575e-06,"Percolator.SVMScore":2.482,"Precursor.Area":251416.1875,"Apex.RT..min.":16.68,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (X4)","Search.ID":"X","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LQETNPEEVPK","Annotated.Sequence":"[K].LQETNPEEVPK.[F]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5404,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":642.32828,"Contaminant":"False","Aligned.m.z..Da.":642.32834,"MH...Da.":1283.64929,"Theo..MH...Da.":1283.64777,"DeltaM..ppm.":1.18,"Deltam.z..Da.":0.00076,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2158131,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":5.25799,"Ion.Inject.Time..ms.":22,"RT..min.":7.6501,"First.Scan":5435,"Last.Scan":5435,"Master.Scan.s.":5429,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_01.raw","File.ID":"F13","Quan.Info":"","Peptides.Matched":521,"XCorr":2.72,"X..Protein.Groups":1,"Percolator.q.Value":0.00021,"Percolator.PEP":6.344e-06,"Percolator.SVMScore":2.186,"Precursor.Area":4009836.75,"Apex.RT..min.":7.67,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (X4)","Search.ID":"X","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"HSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[R].HSDSYsENETNHTNVPISSTGGTNNK.[T]","Modifications":"S6(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0063,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":957.39945,"Contaminant":"False","Aligned.m.z..Da.":957.39954,"MH...Da.":2870.1838,"Theo..MH...Da.":2870.18608,"DeltaM..ppm.":-0.79,"Deltam.z..Da.":-0.00076,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1048220.125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":7.6507,"First.Scan":5436,"Last.Scan":5436,"Master.Scan.s.":5429,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_01.raw","File.ID":"F13","Quan.Info":"","Peptides.Matched":2446,"XCorr":6.37,"X..Protein.Groups":1,"Percolator.q.Value":0.00021,"Percolator.PEP":2.005e-14,"Percolator.SVMScore":6.342,"Precursor.Area":6440471,"Apex.RT..min.":7.66,"ptmRS..Binomial.Peptide.Score":553.45,"ptmRS..Isoform.Confidence.Probability":0.006,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(4): 409.15; b1+-H(3) O(4) P(5): 572.21; b1+-H(3) O(4) P(6): 659.24; b1+-H(3) O(4) P(9): 1031.37; b1+-H(3) O(4) P(10): 1132.42; b1+-H(3) O(4) P(11): 1246.46; b1+-H(3) O(4) P(12): 1383.52; b1+-H(3) O(4) P(15): 1697.68; b2+-H(3) O(4) P(12): 692.26; b2+-H(3) O(4) P(13): 742.79; b2+-H(3) O(4) P(14): 799.81; b2+-H(3) O(4) P(15): 849.34; y2+-H(3) O(4) P(25): 1318.08; y2+-H(3) O(4) P(24): 1274.56; y2+-H(3) O(4) P(23): 1217.05","ptmRS..Best.Site.Probabilities":"S4(Phospho): 99.36","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(4): 99.4; Y(5): 0.0; S(6): 0.6; T(10): 0.0; T(13): 0.0; S(18): 0.0; S(19): 0.0; T(20): 0.0; T(23): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (X4)","Search.ID":"X","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"HSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[R].HSDSySENETNHTNVPISSTGGTNNK.[T]","Modifications":"Y5(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":0,"Charge":4,"Original.Precursor.Charge":4,"DeltaScore":0.0098,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":718.30225,"Contaminant":"False","Aligned.m.z..Da.":718.30232,"MH...Da.":2870.18719,"Theo..MH...Da.":2870.18608,"DeltaM..ppm.":0.39,"Deltam.z..Da.":0.00028,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":4376434,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":7.6578,"First.Scan":5445,"Last.Scan":5445,"Master.Scan.s.":5442,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_01.raw","File.ID":"F13","Quan.Info":"","Peptides.Matched":2546,"XCorr":6.14,"X..Protein.Groups":1,"Percolator.q.Value":0.00021,"Percolator.PEP":4.251e-09,"Percolator.SVMScore":3.738,"Precursor.Area":4175719.75,"Apex.RT..min.":7.66,"ptmRS..Binomial.Peptide.Score":260.87,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(9): 1031.37; b1+-H(3) O(4) P(10): 1132.42; b1+-H(3) O(4) P(11): 1246.46; b1+-H(3) O(4) P(12): 1383.52; b2+-H(3) O(4) P(7): 394.65; b2+-H(3) O(4) P(13): 742.79; b2+-H(3) O(4) P(14): 799.81; b2+-H(3) O(4) P(15): 849.34; b2+-H(3) O(4) P(17): 954.41; b1+(4): 427.16","ptmRS..Best.Site.Probabilities":"S6(Phospho): 99.29","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(4): 0.7; Y(5): 0.0; S(6): 99.3; T(10): 0.0; T(13): 0.0; S(18): 0.0; S(19): 0.0; T(20): 0.0; T(23): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (X4)","Search.ID":"X","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"IQDPQMTGYVSTR","Annotated.Sequence":"[R].IQDPQmTGyVSTR.[Y]","Modifications":"M6(Oxidation); Y9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P32485","Master.Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","Protein.Accessions":"P32485","Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1404,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":796.34451,"Contaminant":"False","Aligned.m.z..Da.":796.34459,"MH...Da.":1591.68175,"Theo..MH...Da.":1591.6822,"DeltaM..ppm.":-0.28,"Deltam.z..Da.":-0.00023,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2647602.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":7.6788,"First.Scan":5475,"Last.Scan":5475,"Master.Scan.s.":5468,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_01.raw","File.ID":"F13","Quan.Info":"","Peptides.Matched":1265,"XCorr":2.85,"X..Protein.Groups":1,"Percolator.q.Value":0.00021,"Percolator.PEP":9.746e-07,"Percolator.SVMScore":2.584,"Precursor.Area":3210760.25,"Apex.RT..min.":7.69,"ptmRS..Binomial.Peptide.Score":277.24,"ptmRS..Isoform.Confidence.Probability":0.988,"ptmRS..Isoform.Group.Report":"y1+(6): 762.32; y1+(5): 705.30","ptmRS..Best.Site.Probabilities":"Y9(Phospho): 98.79","ptmRS..Phospho.Site.Probabilities":"T(7): 0.4; Y(9): 98.8; S(11): 0.4; T(12): 0.4"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (M4)","Search.ID":"M","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0536,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40298,"Contaminant":"False","Aligned.m.z..Da.":967.40298,"MH...Da.":2900.19441,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":-1.7,"Deltam.z..Da.":-0.00164,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":170293.48438,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":7.06103,"Ion.Inject.Time..ms.":22,"RT..min.":14.9283,"First.Scan":15329,"Last.Scan":15329,"Master.Scan.s.":15318,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_06.raw","File.ID":"F24","Quan.Info":"","Peptides.Matched":2254,"XCorr":4.48,"X..Protein.Groups":1,"Percolator.q.Value":0.00021,"Percolator.PEP":1.45e-08,"Percolator.SVMScore":3.192,"Precursor.Area":25301892,"Apex.RT..min.":15.01,"ptmRS..Binomial.Peptide.Score":370.32,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(14): 1621.73; y2+-H(3) O(4) P(14): 811.37; b1+(12): 1344.56","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (M4)","Search.ID":"M","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FTVSAIYSDLPQQER","Annotated.Sequence":"[K].FTVSAIYSDLPQQER.[D]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.684,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":877.43891,"Contaminant":"False","Aligned.m.z..Da.":877.43889,"MH...Da.":1753.87055,"Theo..MH...Da.":1753.87554,"DeltaM..ppm.":-2.85,"Deltam.z..Da.":-0.0025,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":689952.875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":17.30646,"Ion.Inject.Time..ms.":22,"RT..min.":14.9562,"First.Scan":15365,"Last.Scan":15365,"Master.Scan.s.":15357,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_06.raw","File.ID":"F24","Quan.Info":"","Peptides.Matched":1255,"XCorr":4.05,"X..Protein.Groups":1,"Percolator.q.Value":0.00021,"Percolator.PEP":8.204e-10,"Percolator.SVMScore":3.753,"Precursor.Area":882769,"Apex.RT..min.":14.96,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (M4)","Search.ID":"M","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"GVFGYGFEEPSAIQQR","Annotated.Sequence":"[R].GVFGYGFEEPSAIQQR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6981,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":892.93358,"Contaminant":"False","Aligned.m.z..Da.":892.93352,"MH...Da.":1784.8599,"Theo..MH...Da.":1784.86022,"DeltaM..ppm.":-0.18,"Deltam.z..Da.":-0.00016,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":491359.0625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":5.29762,"Ion.Inject.Time..ms.":22,"RT..min.":15.0132,"First.Scan":15438,"Last.Scan":15438,"Master.Scan.s.":15435,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_06.raw","File.ID":"F24","Quan.Info":"","Peptides.Matched":1263,"XCorr":3.61,"X..Protein.Groups":1,"Percolator.q.Value":0.00021,"Percolator.PEP":1.816e-10,"Percolator.SVMScore":4.048,"Precursor.Area":730169.5,"Apex.RT..min.":15.03,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (M4)","Search.ID":"M","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":4,"DeltaScore":0.1049,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40571,"Contaminant":"False","Aligned.m.z..Da.":967.40559,"MH...Da.":2900.20258,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":1.12,"Deltam.z..Da.":0.00108,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3662925.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":35.93751,"Ion.Inject.Time..ms.":22,"RT..min.":15.0978,"First.Scan":15546,"Last.Scan":15546,"Master.Scan.s.":15541,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_06.raw","File.ID":"F24","Quan.Info":"","Peptides.Matched":2558,"XCorr":6.1,"X..Protein.Groups":1,"Percolator.q.Value":0.00021,"Percolator.PEP":3.412e-12,"Percolator.SVMScore":4.824,"Precursor.Area":25301892,"Apex.RT..min.":15.01,"ptmRS..Binomial.Peptide.Score":574.68,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(14): 1528.61; b1+-H(3) O(4) P(16): 1771.69; y1+-H(3) O(4) P(14): 1621.73; y1+-H(3) O(4) P(13): 1458.67; b2+-H(3) O(4) P(16): 886.35; b2+-H(3) O(4) P(18): 1014.90; y2+-H(3) O(4) P(14): 811.37; b1+(12): 1344.56; y1+(13): 1556.65","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (M4)","Search.ID":"M","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"AIMPIIEGHDVLAQAQSGTGK","Annotated.Sequence":"[R].AImPIIEGHDVLAQAQSGTGK.[T]","Modifications":"M3(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.7236,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":718.04006,"Contaminant":"False","Aligned.m.z..Da.":718.04,"MH...Da.":2152.10563,"Theo..MH...Da.":2152.10668,"DeltaM..ppm.":-0.49,"Deltam.z..Da.":-0.00035,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":540914.5625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":18.33809,"Ion.Inject.Time..ms.":22,"RT..min.":12.6188,"First.Scan":12449,"Last.Scan":12449,"Master.Scan.s.":12447,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_06.raw","File.ID":"F24","Quan.Info":"","Peptides.Matched":963,"XCorr":4.74,"X..Protein.Groups":1,"Percolator.q.Value":0.00021,"Percolator.PEP":5.888e-11,"Percolator.SVMScore":4.268,"Precursor.Area":940969.125,"Apex.RT..min.":12.64,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (M4)","Search.ID":"M","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"TGTFSIAALQR","Annotated.Sequence":"[K].TGTFSIAALQR.[I]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7261,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":582.82181,"Contaminant":"False","Aligned.m.z..Da.":582.8219,"MH...Da.":1164.63634,"Theo..MH...Da.":1164.63715,"DeltaM..ppm.":-0.69,"Deltam.z..Da.":-0.0004,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1173300,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":40.49512,"Ion.Inject.Time..ms.":22,"RT..min.":13.4256,"First.Scan":13514,"Last.Scan":13514,"Master.Scan.s.":13513,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_06.raw","File.ID":"F24","Quan.Info":"","Peptides.Matched":380,"XCorr":3.03,"X..Protein.Groups":1,"Percolator.q.Value":0.00021,"Percolator.PEP":1.253e-06,"Percolator.SVMScore":2.321,"Precursor.Area":2726012.25,"Apex.RT..min.":13.4,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (M4)","Search.ID":"M","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].msEGITDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S2(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1888,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1096.46348,"Contaminant":"False","Aligned.m.z..Da.":1096.46289,"MH...Da.":2191.91969,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":-3.72,"Deltam.z..Da.":-0.00408,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":255568.60938,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":11.49956,"Ion.Inject.Time..ms.":22,"RT..min.":15.9497,"First.Scan":16569,"Last.Scan":16569,"Master.Scan.s.":16567,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_06.raw","File.ID":"F24","Quan.Info":"","Peptides.Matched":1887,"XCorr":3.92,"X..Protein.Groups":1,"Percolator.q.Value":0.00021,"Percolator.PEP":6.685e-08,"Percolator.SVMScore":2.894,"Precursor.Area":8129497.5,"Apex.RT..min.":15.7,"ptmRS..Binomial.Peptide.Score":476.32,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(2): 112.04; b1+-H(3) O(4) P(3): 241.08; b1+-H(3) O(4) P(4): 298.10; b1+-H(3) O(4) P(5): 411.19; b1+-H(3) O(4) P(6): 512.24; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+-H(3) O(4) P(9): 869.39; b1+-H(3) O(4) P(10): 998.43; b1+(4): 396.08; y1+(14): 1683.77","ptmRS..Best.Site.Probabilities":"S2(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 100.0; T(6): 0.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (M4)","Search.ID":"M","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0502,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.4065,"Contaminant":"False","Aligned.m.z..Da.":967.40598,"MH...Da.":2900.20495,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":1.94,"Deltam.z..Da.":0.00187,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":251495.64062,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":11.55781,"Ion.Inject.Time..ms.":22,"RT..min.":15.9152,"First.Scan":16529,"Last.Scan":16529,"Master.Scan.s.":16528,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_06.raw","File.ID":"F24","Quan.Info":"","Peptides.Matched":2539,"XCorr":4.98,"X..Protein.Groups":1,"Percolator.q.Value":0.00021,"Percolator.PEP":3.468e-09,"Percolator.SVMScore":3.472,"ptmRS..Binomial.Peptide.Score":362.6,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y2+-H(3) O(4) P(14): 811.37; b1+(12): 1344.56","ptmRS..Best.Site.Probabilities":"S13(Phospho): 99.99","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (M4)","Search.ID":"M","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].mSEGItDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); T6(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.208,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":731.31284,"Contaminant":"False","Aligned.m.z..Da.":731.31257,"MH...Da.":2191.92398,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":-1.76,"Deltam.z..Da.":-0.00129,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":317966.0625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":4.44075,"Ion.Inject.Time..ms.":22,"RT..min.":15.4615,"First.Scan":16020,"Last.Scan":16020,"Master.Scan.s.":16011,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_06.raw","File.ID":"F24","Quan.Info":"","Peptides.Matched":1841,"XCorr":3.27,"X..Protein.Groups":1,"Percolator.q.Value":0.00021,"Percolator.PEP":4.072e-07,"Percolator.SVMScore":2.541,"Precursor.Area":224221.78125,"Apex.RT..min.":15.45,"ptmRS..Binomial.Peptide.Score":297.38,"ptmRS..Isoform.Confidence.Probability":0.995,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+-H(3) O(4) P(9): 869.39; b1+(3): 259.09; b1+(4): 316.11; b1+(5): 429.20","ptmRS..Best.Site.Probabilities":"T6(Phospho): 99.45","ptmRS..Phospho.Site.Probabilities":"S(2): 0.6; T(6): 99.5; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (M4)","Search.ID":"M","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":4,"DeltaScore":0.0525,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40468,"Contaminant":"False","Aligned.m.z..Da.":967.40454,"MH...Da.":2900.19949,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":0.06,"Deltam.z..Da.":0.00005,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2311319.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":17.65677,"Ion.Inject.Time..ms.":22,"RT..min.":15.1303,"First.Scan":15589,"Last.Scan":15589,"Master.Scan.s.":15580,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_06.raw","File.ID":"F24","Quan.Info":"","Peptides.Matched":2481,"XCorr":5.14,"X..Protein.Groups":1,"Percolator.q.Value":0.00021,"Percolator.PEP":6.608e-11,"Percolator.SVMScore":4.245,"Precursor.Area":25301892,"Apex.RT..min.":15.01,"ptmRS..Binomial.Peptide.Score":342.98,"ptmRS..Isoform.Confidence.Probability":0.993,"ptmRS..Isoform.Group.Report":"b1+(12): 1344.56","ptmRS..Best.Site.Probabilities":"S13(Phospho): 99.26","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.7; S(13): 99.3; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (M4)","Search.ID":"M","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0255,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.4055,"Contaminant":"False","Aligned.m.z..Da.":967.40516,"MH...Da.":2900.20195,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":0.9,"Deltam.z..Da.":0.00087,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1048500,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":6.22413,"Ion.Inject.Time..ms.":22,"RT..min.":15.4177,"First.Scan":15960,"Last.Scan":15960,"Master.Scan.s.":15959,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_06.raw","File.ID":"F24","Quan.Info":"","Peptides.Matched":2539,"XCorr":5.1,"X..Protein.Groups":1,"Percolator.q.Value":0.00021,"Percolator.PEP":5.529e-09,"Percolator.SVMScore":3.381,"Precursor.Area":25301892,"Apex.RT..min.":15.01,"ptmRS..Binomial.Peptide.Score":456.8,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(14): 1528.61; b1+-H(3) O(4) P(16): 1771.69; y1+-H(3) O(4) P(14): 1621.73; y1+-H(3) O(4) P(13): 1458.67; b1+(12): 1344.56","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (M4)","Search.ID":"M","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].mSEGItDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); T6(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1118,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1096.46697,"Contaminant":"False","Aligned.m.z..Da.":1096.46657,"MH...Da.":2191.92668,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":-0.53,"Deltam.z..Da.":-0.00058,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":389618.96875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":15.4476,"First.Scan":16000,"Last.Scan":16000,"Master.Scan.s.":15998,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_06.raw","File.ID":"F24","Quan.Info":"","Peptides.Matched":1944,"XCorr":5.01,"X..Protein.Groups":1,"Percolator.q.Value":0.00021,"Percolator.PEP":2.629e-09,"Percolator.SVMScore":3.526,"Precursor.Area":380613.4375,"Apex.RT..min.":15.45,"ptmRS..Binomial.Peptide.Score":478.66,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(6): 512.24; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+-H(3) O(4) P(9): 869.39; b1+-H(3) O(4) P(10): 998.43; y1+-H(3) O(4) P(14): 1665.76; y2+-H(3) O(4) P(14): 833.38; b1+(3): 259.09; b1+(4): 316.11; b1+(5): 429.20","ptmRS..Best.Site.Probabilities":"T6(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; T(6): 100.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (M4)","Search.ID":"M","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DIFSNDELLSDAYDAK","Annotated.Sequence":"[K].DIFSNDELLSDAYDAK.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6869,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":908.41939,"Contaminant":"False","Aligned.m.z..Da.":908.41909,"MH...Da.":1815.83151,"Theo..MH...Da.":1815.82831,"DeltaM..ppm.":1.76,"Deltam.z..Da.":0.0016,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":868853.1875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":21.63408,"Ion.Inject.Time..ms.":22,"RT..min.":17.0639,"First.Scan":17673,"Last.Scan":17673,"Master.Scan.s.":17670,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_06.raw","File.ID":"F24","Quan.Info":"","Peptides.Matched":1695,"XCorr":3.96,"X..Protein.Groups":1,"Percolator.q.Value":0.00021,"Percolator.PEP":4.46e-10,"Percolator.SVMScore":3.872,"Precursor.Area":2203297.75,"Apex.RT..min.":17.09,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (M4)","Search.ID":"M","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0272,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40536,"Contaminant":"False","Aligned.m.z..Da.":967.40506,"MH...Da.":2900.20153,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":0.76,"Deltam.z..Da.":0.00073,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":95802.96094,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":9.29683,"Ion.Inject.Time..ms.":22,"RT..min.":17.1727,"First.Scan":17773,"Last.Scan":17773,"Master.Scan.s.":17761,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_06.raw","File.ID":"F24","Quan.Info":"","Peptides.Matched":2519,"XCorr":3.67,"X..Protein.Groups":1,"Percolator.q.Value":0.00021,"Percolator.PEP":3.776e-08,"Percolator.SVMScore":3.005,"Precursor.Area":463439.65625,"Apex.RT..min.":16.69,"ptmRS..Binomial.Peptide.Score":325.08,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b2+-H(3) O(4) P(17): 950.38; b2+-H(3) O(4) P(18): 1014.90","ptmRS..Best.Site.Probabilities":"S13(Phospho): 99.99","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (M4)","Search.ID":"M","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0025,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.4065,"Contaminant":"False","Aligned.m.z..Da.":967.40603,"MH...Da.":2900.20495,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":1.94,"Deltam.z..Da.":0.00187,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":127014.82031,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":12.62895,"Ion.Inject.Time..ms.":22,"RT..min.":16.4695,"First.Scan":17115,"Last.Scan":17115,"Master.Scan.s.":17112,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_06.raw","File.ID":"F24","Quan.Info":"","Peptides.Matched":2539,"XCorr":3.98,"X..Protein.Groups":1,"Percolator.q.Value":0.00021,"Percolator.PEP":2.462e-07,"Percolator.SVMScore":2.639,"Precursor.Area":463439.65625,"Apex.RT..min.":16.69,"ptmRS..Binomial.Peptide.Score":338.75,"ptmRS..Isoform.Confidence.Probability":0.5,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"Y12(Phospho): 50; S13(Phospho): 50","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 50.0; S(13): 50.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (M4)","Search.ID":"M","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MFILDEADEMLSSGFK","Annotated.Sequence":"[K].mFILDEADEmLSSGFK.[E]","Modifications":"M1(Oxidation); M10(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5228,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":932.92208,"Contaminant":"False","Aligned.m.z..Da.":932.92167,"MH...Da.":1864.83688,"Theo..MH...Da.":1864.83433,"DeltaM..ppm.":1.37,"Deltam.z..Da.":0.00128,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":329443,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":24.77125,"Ion.Inject.Time..ms.":22,"RT..min.":16.6685,"First.Scan":17303,"Last.Scan":17303,"Master.Scan.s.":17296,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_06.raw","File.ID":"F24","Quan.Info":"","Peptides.Matched":1899,"XCorr":2.85,"X..Protein.Groups":1,"Percolator.q.Value":0.00021,"Percolator.PEP":6.741e-08,"Percolator.SVMScore":2.892,"Precursor.Area":822176,"Apex.RT..min.":16.7,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (M4)","Search.ID":"M","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"HSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[R].HSDsYsENETNHTNVPISSTGGTNNK.[T]","Modifications":"S4(Phospho); S6(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.1455,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":984.05562,"Contaminant":"False","Aligned.m.z..Da.":984.05569,"MH...Da.":2950.15231,"Theo..MH...Da.":2950.15241,"DeltaM..ppm.":-0.03,"Deltam.z..Da.":-0.00003,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3303375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":7.9473,"First.Scan":6158,"Last.Scan":6158,"Master.Scan.s.":6155,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_06.raw","File.ID":"F24","Quan.Info":"","Peptides.Matched":1116,"XCorr":6.39,"X..Protein.Groups":1,"Percolator.q.Value":0.00021,"Percolator.PEP":4.49e-10,"Percolator.SVMScore":3.871,"Precursor.Area":4563262.5,"Apex.RT..min.":7.96,"ptmRS..Binomial.Peptide.Score":611.7,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(6): 739.21; b1+-H(3) O(4) P(9): 1111.34; y2+-H(3) O(4) P(25): 1358.06; y2+-H(3) O(4) P(23): 1257.03; b1+- -2H(3) O(4) P -2H(3) O(4) P(6): 641.23; b1+- -2H(3) O(4) P -2H(3) O(4) P(11): 1228.45; b1+- -2H(3) O(4) P -2H(3) O(4) P(15): 1679.67; b2+- -2H(3) O(4) P -2H(3) O(4) P(13): 733.78; b2+- -2H(3) O(4) P -2H(3) O(4) P(14): 790.80; b2+- -2H(3) O(4) P -2H(3) O(4) P(15): 840.34","ptmRS..Best.Site.Probabilities":"S4(Phospho): 100; S6(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(4): 100.0; Y(5): 0.0; S(6): 100.0; T(10): 0.0; T(13): 0.0; S(18): 0.0; S(19): 0.0; T(20): 0.0; T(23): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (M4)","Search.ID":"M","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"DFRHSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[K].DFRHSDsYsENETNHTNVPISSTGGTNNK.[T]","Modifications":"S7(Phospho); S9(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":1,"Charge":4,"Original.Precursor.Charge":4,"DeltaScore":0.0152,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":842.84063,"Contaminant":"False","Aligned.m.z..Da.":842.84066,"MH...Da.":3368.34072,"Theo..MH...Da.":3368.34888,"DeltaM..ppm.":-2.42,"Deltam.z..Da.":-0.00204,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1079228.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":33.59562,"Ion.Inject.Time..ms.":22,"RT..min.":8.6095,"First.Scan":7041,"Last.Scan":7041,"Master.Scan.s.":7039,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_06.raw","File.ID":"F24","Quan.Info":"","Peptides.Matched":904,"XCorr":6.58,"X..Protein.Groups":1,"Percolator.q.Value":0.00021,"Percolator.PEP":3.784e-08,"Percolator.SVMScore":3.005,"Precursor.Area":2567552.25,"Apex.RT..min.":8.62,"ptmRS..Binomial.Peptide.Score":407.45,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"b2+-H(3) O(4) P(7): 454.16; b2+-H(3) O(4) P(8): 535.69; b2+- -2H(3) O(4) P -2H(3) O(4) P(15): 892.36; b2+- -2H(3) O(4) P -2H(3) O(4) P(17): 999.90; b2+- -2H(3) O(4) P -2H(3) O(4) P(18): 1049.44","ptmRS..Best.Site.Probabilities":"S5(Phospho): 100; S7(Phospho): 98.82","ptmRS..Phospho.Site.Probabilities":"S(5): 100.0; S(7): 98.8; Y(8): 0.0; S(9): 1.2; T(13): 0.0; T(16): 0.0; S(21): 0.0; S(22): 0.0; T(23): 0.0; T(26): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (M4)","Search.ID":"M","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DFRHSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[K].DFRHsDsYSENETNHTNVPISSTGGTNNK.[T]","Modifications":"S5(Phospho); S7(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.1266,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1123.45421,"Contaminant":"False","Aligned.m.z..Da.":1123.45425,"MH...Da.":3368.34809,"Theo..MH...Da.":3368.34888,"DeltaM..ppm.":-0.23,"Deltam.z..Da.":-0.00026,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1837015.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":24.22469,"Ion.Inject.Time..ms.":22,"RT..min.":8.6291,"First.Scan":7067,"Last.Scan":7067,"Master.Scan.s.":7065,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_06.raw","File.ID":"F24","Quan.Info":"","Peptides.Matched":927,"XCorr":4.66,"X..Protein.Groups":1,"Percolator.q.Value":0.00021,"Percolator.PEP":5.309e-09,"Percolator.SVMScore":3.388,"Precursor.Area":1276332,"Apex.RT..min.":8.62,"ptmRS..Binomial.Peptide.Score":444.66,"ptmRS..Isoform.Confidence.Probability":0.333,"ptmRS..Isoform.Group.Report":"y2+-H(3) O(4) P(24): 1274.56; y2+-H(3) O(4) P(23): 1217.05; b2+- -2H(3) O(4) P -2H(3) O(4) P(15): 892.36; b2+- -2H(3) O(4) P -2H(3) O(4) P(17): 999.90; b2+- -2H(3) O(4) P -2H(3) O(4) P(18): 1049.44; b2+- -2H(3) O(4) P -2H(3) O(4) P(20): 1154.50","ptmRS..Best.Site.Probabilities":"S5(Phospho): 100; S7(Phospho): 33.33; S9(Phospho): 33.33; T13(Phospho): 33.33","ptmRS..Phospho.Site.Probabilities":"S(5): 100.0; S(7): 33.3; Y(8): 0.0; S(9): 33.3; T(13): 33.3; T(16): 0.0; S(21): 0.0; S(22): 0.0; T(23): 0.0; T(26): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (M4)","Search.ID":"M","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MGSINNNFNTNNNSNTDLDRDWK","Annotated.Sequence":"[-].mGSINNNFNTNNNsNTDLDRDWK.[T]","Modifications":"N-Term(Prot)(Met-loss); S14(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P39517","Master.Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","Protein.Accessions":"P39517","Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0828,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":878.37009,"Contaminant":"False","Aligned.m.z..Da.":878.36996,"MH...Da.":2633.09574,"Theo..MH...Da.":2633.10123,"DeltaM..ppm.":-2.08,"Deltam.z..Da.":-0.00183,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1490485.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":11.2375,"First.Scan":10604,"Last.Scan":10604,"Master.Scan.s.":10601,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_06.raw","File.ID":"F24","Quan.Info":"","Peptides.Matched":2160,"XCorr":4.95,"X..Protein.Groups":1,"Percolator.q.Value":0.00021,"Percolator.PEP":1.443e-10,"Percolator.SVMScore":4.093,"Precursor.Area":1739103.25,"Apex.RT..min.":11.24,"ptmRS..Binomial.Peptide.Score":515.03,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(12): 1459.66; y1+-H(3) O(4) P(11): 1345.61; y1+-H(3) O(4) P(10): 1231.57; y2+-H(3) O(4) P(17): 1025.45; y2+-H(3) O(4) P(16): 968.43; y2+-H(3) O(4) P(15): 894.90; y2+-H(3) O(4) P(14): 837.88; y2+-H(3) O(4) P(12): 730.33; y2+-H(3) O(4) P(11): 673.31; y2+-H(3) O(4) P(10): 616.29; y1+(9): 1162.55; y1+(8): 1048.51; y2+(9): 581.78","ptmRS..Best.Site.Probabilities":"S14(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(3): 0.0; T(10): 0.0; S(14): 100.0; T(16): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (M4)","Search.ID":"M","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KDELTLEGIK","Annotated.Sequence":"[K].KDELTLEGIK.[Q]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.6186,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":382.552,"Contaminant":"False","Aligned.m.z..Da.":382.55202,"MH...Da.":1145.64146,"Theo..MH...Da.":1145.64123,"DeltaM..ppm.":0.2,"Deltam.z..Da.":0.00008,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1606646.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":34.33363,"Ion.Inject.Time..ms.":22,"RT..min.":10.265,"First.Scan":9256,"Last.Scan":9256,"Master.Scan.s.":9249,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_06.raw","File.ID":"F24","Quan.Info":"","Peptides.Matched":307,"XCorr":2.91,"X..Protein.Groups":1,"Percolator.q.Value":0.00021,"Percolator.PEP":0.00003,"Percolator.SVMScore":1.715,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (M4)","Search.ID":"M","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KDELTLEGIK","Annotated.Sequence":"[K].KDELTLEGIK.[Q]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":573.32454,"Contaminant":"False","Aligned.m.z..Da.":573.32456,"MH...Da.":1145.64181,"Theo..MH...Da.":1145.64123,"DeltaM..ppm.":0.51,"Deltam.z..Da.":0.00029,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2407468.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":8.2639,"Ion.Inject.Time..ms.":22,"RT..min.":10.2627,"First.Scan":9252,"Last.Scan":9252,"Master.Scan.s.":9249,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_06.raw","File.ID":"F24","Quan.Info":"","Peptides.Matched":302,"XCorr":3.82,"X..Protein.Groups":1,"Percolator.q.Value":0.00021,"Percolator.PEP":6.774e-08,"Percolator.SVMScore":2.891,"Precursor.Area":2536595,"Apex.RT..min.":10.27,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (M4)","Search.ID":"M","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"AGNFALHPEVVR","Annotated.Sequence":"[R].AGNFALHPEVVR.[E]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q03558","Master.Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","Protein.Accessions":"Q03558","Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.7,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":437.23755,"Contaminant":"False","Aligned.m.z..Da.":437.23757,"MH...Da.":1309.6981,"Theo..MH...Da.":1309.70114,"DeltaM..ppm.":-2.33,"Deltam.z..Da.":-0.00102,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1703412.875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":11.36625,"Ion.Inject.Time..ms.":22,"RT..min.":10.0353,"First.Scan":8944,"Last.Scan":8944,"Master.Scan.s.":8937,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_06.raw","File.ID":"F24","Quan.Info":"","Peptides.Matched":436,"XCorr":2.9,"X..Protein.Groups":1,"Percolator.q.Value":0.00021,"Percolator.PEP":1.904e-07,"Percolator.SVMScore":2.689,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (M4)","Search.ID":"M","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"APQALMLAPTR","Annotated.Sequence":"[K].APQALmLAPTR.[E]","Modifications":"M6(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7492,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":592.82636,"Contaminant":"False","Aligned.m.z..Da.":592.82638,"MH...Da.":1184.64545,"Theo..MH...Da.":1184.6456,"DeltaM..ppm.":-0.13,"Deltam.z..Da.":-0.00008,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1734662.125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":16.42075,"Ion.Inject.Time..ms.":22,"RT..min.":10.1905,"First.Scan":9155,"Last.Scan":9155,"Master.Scan.s.":9145,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_06.raw","File.ID":"F24","Quan.Info":"","Peptides.Matched":307,"XCorr":3.07,"X..Protein.Groups":1,"Percolator.q.Value":0.00021,"Percolator.PEP":2.137e-06,"Percolator.SVMScore":2.217,"Precursor.Area":2685666,"Apex.RT..min.":10.21,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (X4)","Search.ID":"X","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FDDMELDENLLR","Annotated.Sequence":"[K].FDDmELDENLLR.[G]","Modifications":"M4(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":3,"DeltaScore":0.6069,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":763.34499,"Contaminant":"False","Aligned.m.z..Da.":763.34518,"MH...Da.":1525.6827,"Theo..MH...Da.":1525.6839,"DeltaM..ppm.":-0.79,"Deltam.z..Da.":-0.0006,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":988268.6875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":29.85094,"Ion.Inject.Time..ms.":22,"RT..min.":14.4867,"First.Scan":13875,"Last.Scan":13875,"Master.Scan.s.":13864,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_01.raw","File.ID":"F13","Quan.Info":"","Peptides.Matched":1109,"XCorr":2.9,"X..Protein.Groups":1,"Percolator.q.Value":0.00021,"Percolator.PEP":9.561e-07,"Percolator.SVMScore":2.588,"Precursor.Area":718749.5625,"Apex.RT..min.":14.48,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (M4)","Search.ID":"M","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"AAEAGETGAATSATEGDNNNNTAAGDKK","Annotated.Sequence":"[K].AAEAGETGAATSATEGDNNNNTAAGDKK.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.7481,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":879.39358,"Contaminant":"False","Aligned.m.z..Da.":879.39348,"MH...Da.":2636.16621,"Theo..MH...Da.":2636.16665,"DeltaM..ppm.":-0.17,"Deltam.z..Da.":-0.00015,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3477933.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":1.75982,"Ion.Inject.Time..ms.":22,"RT..min.":5.023,"First.Scan":2040,"Last.Scan":2040,"Master.Scan.s.":2034,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_06.raw","File.ID":"F24","Quan.Info":"","Peptides.Matched":3592,"XCorr":7.7,"X..Protein.Groups":1,"Percolator.q.Value":0.00021,"Percolator.PEP":7.04783e-18,"Percolator.SVMScore":9.65498,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (K4)","Search.ID":"K","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"AAEAGETGAATSATEGDNNNNTAAGDKK","Annotated.Sequence":"[K].AAEAGETGAATSATEGDNNNNTAAGDKK.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.7549,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":879.39388,"Contaminant":"False","Aligned.m.z..Da.":879.39382,"MH...Da.":2636.16708,"Theo..MH...Da.":2636.16665,"DeltaM..ppm.":0.16,"Deltam.z..Da.":0.00014,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3821623,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":4.9135,"First.Scan":1682,"Last.Scan":1682,"Master.Scan.s.":1681,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_02.raw","File.ID":"F26","Quan.Info":"","Peptides.Matched":3587,"XCorr":8.24,"X..Protein.Groups":1,"Percolator.q.Value":0.00022,"Percolator.PEP":6.30512e-16,"Percolator.SVMScore":11.75871,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (K4)","Search.ID":"K","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].mSEGItDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); T6(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.1922,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":731.31317,"Contaminant":"False","Aligned.m.z..Da.":731.31309,"MH...Da.":2191.92496,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":-1.32,"Deltam.z..Da.":-0.00096,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":418796.71875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":4.10006,"Ion.Inject.Time..ms.":22,"RT..min.":15.5151,"First.Scan":15481,"Last.Scan":15481,"Master.Scan.s.":15474,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_02.raw","File.ID":"F26","Quan.Info":"","Peptides.Matched":1913,"XCorr":3.59,"X..Protein.Groups":1,"Percolator.q.Value":0.00022,"Percolator.PEP":6.041e-08,"Percolator.SVMScore":2.986,"Precursor.Area":407274.4375,"Apex.RT..min.":15.53,"ptmRS..Binomial.Peptide.Score":338.93,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+-H(3) O(4) P(9): 869.39; b1+(2): 130.05; b1+(3): 259.09; b1+(4): 316.11; b1+(5): 429.20; b2+(3): 130.05","ptmRS..Best.Site.Probabilities":"T6(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; T(6): 100.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (K4)","Search.ID":"K","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].mSEGItDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); T6(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.0972,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1096.46734,"Contaminant":"False","Aligned.m.z..Da.":1096.46723,"MH...Da.":2191.92742,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":-0.2,"Deltam.z..Da.":-0.00021,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":622723.0625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":15.5121,"First.Scan":15476,"Last.Scan":15476,"Master.Scan.s.":15474,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_02.raw","File.ID":"F26","Quan.Info":"","Peptides.Matched":1941,"XCorr":5.04,"X..Protein.Groups":1,"Percolator.q.Value":0.00022,"Percolator.PEP":1.346e-11,"Percolator.SVMScore":4.646,"Precursor.Area":639012.8125,"Apex.RT..min.":15.53,"ptmRS..Binomial.Peptide.Score":483.49,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(6): 512.24; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+-H(3) O(4) P(9): 869.39; b1+-H(3) O(4) P(10): 998.43; y2+-H(3) O(4) P(14): 833.38; b1+(3): 259.09; b1+(4): 316.11; b1+(5): 429.20","ptmRS..Best.Site.Probabilities":"T6(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; T(6): 100.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (K4)","Search.ID":"K","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0041,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40638,"Contaminant":"False","Aligned.m.z..Da.":967.40629,"MH...Da.":2900.2046,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":1.82,"Deltam.z..Da.":0.00176,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":632677.1875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":8.29447,"Ion.Inject.Time..ms.":22,"RT..min.":15.4917,"First.Scan":15450,"Last.Scan":15450,"Master.Scan.s.":15448,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_02.raw","File.ID":"F26","Quan.Info":"","Peptides.Matched":2546,"XCorr":4.9,"X..Protein.Groups":1,"Percolator.q.Value":0.00022,"Percolator.PEP":1.299e-08,"Percolator.SVMScore":3.289,"ptmRS..Binomial.Peptide.Score":351.36,"ptmRS..Isoform.Confidence.Probability":0.993,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(15): 1642.65","ptmRS..Best.Site.Probabilities":"S13(Phospho): 99.32","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.7; S(13): 99.3; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (K4)","Search.ID":"K","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":5,"DeltaScore":0.075,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40449,"Contaminant":"False","Aligned.m.z..Da.":967.40444,"MH...Da.":2900.19893,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":-0.14,"Deltam.z..Da.":-0.00013,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":9960128,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":7.76672,"Ion.Inject.Time..ms.":19.214,"RT..min.":15.1351,"First.Scan":14996,"Last.Scan":14996,"Master.Scan.s.":14993,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_02.raw","File.ID":"F26","Quan.Info":"","Peptides.Matched":2483,"XCorr":6,"X..Protein.Groups":1,"Percolator.q.Value":0.00022,"Percolator.PEP":6.305e-16,"Percolator.SVMScore":6.67,"Precursor.Area":42622500,"Apex.RT..min.":15.09,"ptmRS..Binomial.Peptide.Score":729.94,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(13): 1413.58; b1+-H(3) O(4) P(14): 1528.61; b1+-H(3) O(4) P(16): 1771.69; y1+-H(3) O(4) P(14): 1621.73; y1+-H(3) O(4) P(13): 1458.67; b2+-H(3) O(4) P(16): 886.35; b2+-H(3) O(4) P(18): 1014.90; y2+-H(3) O(4) P(14): 811.37; b1+(12): 1344.56; y1+(13): 1556.65","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (K4)","Search.ID":"K","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].mSEGITDIEEsQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S11(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1455,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1096.46774,"Contaminant":"False","Aligned.m.z..Da.":1096.46771,"MH...Da.":2191.92821,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":0.17,"Deltam.z..Da.":0.00018,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":718186.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":13.95242,"Ion.Inject.Time..ms.":22,"RT..min.":15.0047,"First.Scan":14828,"Last.Scan":14828,"Master.Scan.s.":14824,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_02.raw","File.ID":"F26","Quan.Info":"","Peptides.Matched":2009,"XCorr":3.85,"X..Protein.Groups":1,"Percolator.q.Value":0.00022,"Percolator.PEP":9.347e-10,"Percolator.SVMScore":3.809,"Precursor.Area":329984.84375,"Apex.RT..min.":15,"ptmRS..Binomial.Peptide.Score":407.46,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(14): 1665.76; y1+-H(3) O(4) P(13): 1564.71; y1+-H(3) O(4) P(12): 1449.69; y1+-H(3) O(4) P(11): 1336.60; y1+-H(3) O(4) P(10): 1207.56; y1+-H(3) O(4) P(9): 1078.52; y2+-H(3) O(4) P(18): 1026.47; b1+(6): 530.25; b1+(8): 758.36","ptmRS..Best.Site.Probabilities":"S11(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; T(6): 0.0; S(11): 100.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (K4)","Search.ID":"K","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"GVFGYGFEEPSAIQQR","Annotated.Sequence":"[R].GVFGYGFEEPSAIQQR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6785,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":892.93365,"Contaminant":"False","Aligned.m.z..Da.":892.93362,"MH...Da.":1784.86003,"Theo..MH...Da.":1784.86022,"DeltaM..ppm.":-0.11,"Deltam.z..Da.":-0.0001,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":947793.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":6.14435,"Ion.Inject.Time..ms.":22,"RT..min.":15.0147,"First.Scan":14841,"Last.Scan":14841,"Master.Scan.s.":14837,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_02.raw","File.ID":"F26","Quan.Info":"","Peptides.Matched":1263,"XCorr":4.23,"X..Protein.Groups":1,"Percolator.q.Value":0.00022,"Percolator.PEP":1.775e-12,"Percolator.SVMScore":5.047,"Precursor.Area":1405837.25,"Apex.RT..min.":15.03,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (K4)","Search.ID":"K","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FTVSAIYSDLPQQER","Annotated.Sequence":"[K].FTVSAIYSDLPQQER.[D]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6777,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":877.44117,"Contaminant":"False","Aligned.m.z..Da.":877.44114,"MH...Da.":1753.87508,"Theo..MH...Da.":1753.87554,"DeltaM..ppm.":-0.26,"Deltam.z..Da.":-0.00023,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":733259.1875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":11.51238,"Ion.Inject.Time..ms.":22,"RT..min.":15.0153,"First.Scan":14842,"Last.Scan":14842,"Master.Scan.s.":14837,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_02.raw","File.ID":"F26","Quan.Info":"","Peptides.Matched":1095,"XCorr":3.63,"X..Protein.Groups":1,"Percolator.q.Value":0.00022,"Percolator.PEP":2.849e-11,"Percolator.SVMScore":4.498,"Precursor.Area":1016111.125,"Apex.RT..min.":15.03,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (K4)","Search.ID":"K","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FFISNPDLVDRLEK","Annotated.Sequence":"[R].FFISNPDLVDRLEK.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q03558","Master.Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","Protein.Accessions":"Q03558","Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.7474,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":564.97002,"Contaminant":"False","Aligned.m.z..Da.":564.96998,"MH...Da.":1692.89553,"Theo..MH...Da.":1692.89555,"DeltaM..ppm.":-0.01,"Deltam.z..Da.":-1e-05,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":335811.375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":25.70128,"Ion.Inject.Time..ms.":22,"RT..min.":15.3231,"First.Scan":15237,"Last.Scan":15237,"Master.Scan.s.":15227,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_02.raw","File.ID":"F26","Quan.Info":"","Peptides.Matched":551,"XCorr":2.85,"X..Protein.Groups":1,"Percolator.q.Value":0.00022,"Percolator.PEP":5.254e-07,"Percolator.SVMScore":2.559,"Precursor.Area":394672.78125,"Apex.RT..min.":15.33,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (K4)","Search.ID":"K","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":2,"DeltaScore":0.0584,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.4078,"Contaminant":"False","Aligned.m.z..Da.":967.40774,"MH...Da.":2900.20884,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":3.28,"Deltam.z..Da.":0.00317,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":4329243.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":30.10837,"Ion.Inject.Time..ms.":22,"RT..min.":15.192,"First.Scan":15070,"Last.Scan":15070,"Master.Scan.s.":15058,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_02.raw","File.ID":"F26","Quan.Info":"","Peptides.Matched":2619,"XCorr":5.31,"X..Protein.Groups":1,"Percolator.q.Value":0.00022,"Percolator.PEP":5.534e-08,"Percolator.SVMScore":3.003,"Precursor.Area":742251.4375,"Apex.RT..min.":14.83,"ptmRS..Binomial.Peptide.Score":450.75,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(13): 1413.58; b1+-H(3) O(4) P(14): 1528.61; y1+-H(3) O(4) P(15): 1750.78; y1+-H(3) O(4) P(14): 1621.73; y1+-H(3) O(4) P(13): 1458.67; b1+(12): 1344.56","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (K4)","Search.ID":"K","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].msEGITDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S2(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1505,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1096.46508,"Contaminant":"False","Aligned.m.z..Da.":1096.46493,"MH...Da.":2191.92289,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":-2.26,"Deltam.z..Da.":-0.00248,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":403170.4375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":16.0231,"First.Scan":16126,"Last.Scan":16126,"Master.Scan.s.":16124,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_02.raw","File.ID":"F26","Quan.Info":"","Peptides.Matched":1912,"XCorr":4.12,"X..Protein.Groups":1,"Percolator.q.Value":0.00022,"Percolator.PEP":6.355e-09,"Percolator.SVMScore":3.43,"Precursor.Area":21676970,"Apex.RT..min.":15.78,"ptmRS..Binomial.Peptide.Score":478.66,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(2): 112.04; b1+-H(3) O(4) P(3): 241.08; b1+-H(3) O(4) P(4): 298.10; b1+-H(3) O(4) P(5): 411.19; b1+-H(3) O(4) P(6): 512.24; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+-H(3) O(4) P(9): 869.39; b1+-H(3) O(4) P(10): 998.43; b1+(4): 396.08; y1+(14): 1683.77","ptmRS..Best.Site.Probabilities":"S2(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 100.0; T(6): 0.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (K4)","Search.ID":"K","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.3883,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40412,"Contaminant":"False","Aligned.m.z..Da.":967.40398,"MH...Da.":2900.19781,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":-0.52,"Deltam.z..Da.":-0.0005,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":320372.375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":24.01814,"Ion.Inject.Time..ms.":22,"RT..min.":16.0807,"First.Scan":16201,"Last.Scan":16201,"Master.Scan.s.":16189,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_02.raw","File.ID":"F26","Quan.Info":"","Peptides.Matched":2423,"XCorr":3.94,"X..Protein.Groups":1,"Percolator.q.Value":0.00022,"Percolator.PEP":3.464e-08,"Percolator.SVMScore":3.096,"Precursor.Area":554507.6875,"Apex.RT..min.":15.99,"ptmRS..Binomial.Peptide.Score":313.74,"ptmRS..Isoform.Confidence.Probability":0.991,"ptmRS..Isoform.Group.Report":"y2+-H(3) O(4) P(14): 811.37","ptmRS..Best.Site.Probabilities":"S13(Phospho): 99.13","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.9; S(13): 99.1; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (K4)","Search.ID":"K","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEySDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); Y12(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.3705,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.4043,"Contaminant":"False","Aligned.m.z..Da.":967.40416,"MH...Da.":2900.19835,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":-0.34,"Deltam.z..Da.":-0.00033,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":539527.125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":18.70664,"Ion.Inject.Time..ms.":22,"RT..min.":16.0027,"First.Scan":16100,"Last.Scan":16100,"Master.Scan.s.":16098,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_02.raw","File.ID":"F26","Quan.Info":"","Peptides.Matched":2434,"XCorr":4.75,"X..Protein.Groups":1,"Percolator.q.Value":0.00022,"Percolator.PEP":2.45e-08,"Percolator.SVMScore":3.164,"Precursor.Area":554507.6875,"Apex.RT..min.":15.99,"ptmRS..Binomial.Peptide.Score":315.18,"ptmRS..Isoform.Confidence.Probability":0.5,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"Y12(Phospho): 50; S13(Phospho): 50","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 50.0; S(13): 50.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (K4)","Search.ID":"K","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"HSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[R].HSDSYsENETNHTNVPISSTGGTNNK.[T]","Modifications":"S6(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0106,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":957.40043,"Contaminant":"False","Aligned.m.z..Da.":957.40057,"MH...Da.":2870.18676,"Theo..MH...Da.":2870.18608,"DeltaM..ppm.":0.24,"Deltam.z..Da.":0.00023,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1693206.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":7.6561,"First.Scan":5458,"Last.Scan":5458,"Master.Scan.s.":5451,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_02.raw","File.ID":"F26","Quan.Info":"","Peptides.Matched":2517,"XCorr":7.53,"X..Protein.Groups":1,"Percolator.q.Value":0.00022,"Percolator.PEP":6.305e-16,"Percolator.SVMScore":7.044,"Precursor.Area":12872731,"Apex.RT..min.":7.67,"ptmRS..Binomial.Peptide.Score":559.54,"ptmRS..Isoform.Confidence.Probability":0.006,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(4): 409.15; b1+-H(3) O(4) P(5): 572.21; b1+-H(3) O(4) P(6): 659.24; b1+-H(3) O(4) P(9): 1031.37; b1+-H(3) O(4) P(10): 1132.42; b1+-H(3) O(4) P(11): 1246.46; b1+-H(3) O(4) P(12): 1383.52; b1+-H(3) O(4) P(15): 1697.68; b2+-H(3) O(4) P(12): 692.26; b2+-H(3) O(4) P(13): 742.79; b2+-H(3) O(4) P(14): 799.81; b2+-H(3) O(4) P(15): 849.34; y2+-H(3) O(4) P(25): 1318.08; y2+-H(3) O(4) P(24): 1274.56; y2+-H(3) O(4) P(23): 1217.05","ptmRS..Best.Site.Probabilities":"S4(Phospho): 99.39","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(4): 99.4; Y(5): 0.0; S(6): 0.6; T(10): 0.0; T(13): 0.0; S(18): 0.0; S(19): 0.0; T(20): 0.0; T(23): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (K4)","Search.ID":"K","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"IQDPQMTGYVSTR","Annotated.Sequence":"[R].IQDPQmTGyVSTR.[Y]","Modifications":"M6(Oxidation); Y9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P32485","Master.Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","Protein.Accessions":"P32485","Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1111,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":796.34442,"Contaminant":"False","Aligned.m.z..Da.":796.34454,"MH...Da.":1591.68157,"Theo..MH...Da.":1591.6822,"DeltaM..ppm.":-0.4,"Deltam.z..Da.":-0.00031,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2505820.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":7.6572,"First.Scan":5460,"Last.Scan":5460,"Master.Scan.s.":5451,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_02.raw","File.ID":"F26","Quan.Info":"","Peptides.Matched":1281,"XCorr":3.24,"X..Protein.Groups":1,"Percolator.q.Value":0.00022,"Percolator.PEP":4.708e-08,"Percolator.SVMScore":3.035,"Precursor.Area":6462827,"Apex.RT..min.":7.69,"ptmRS..Binomial.Peptide.Score":277.95,"ptmRS..Isoform.Confidence.Probability":0.992,"ptmRS..Isoform.Group.Report":"y1+(6): 762.32; y1+(5): 705.30","ptmRS..Best.Site.Probabilities":"Y9(Phospho): 99.2","ptmRS..Phospho.Site.Probabilities":"T(7): 0.4; Y(9): 99.2; S(11): 0.4; T(12): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (K4)","Search.ID":"K","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"HSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[R].HSDSySENETNHTNVPISSTGGTNNK.[T]","Modifications":"Y5(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":0,"Charge":4,"Original.Precursor.Charge":4,"DeltaScore":0.0048,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":718.30121,"Contaminant":"False","Aligned.m.z..Da.":718.30131,"MH...Da.":2870.18301,"Theo..MH...Da.":2870.18608,"DeltaM..ppm.":-1.07,"Deltam.z..Da.":-0.00077,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":6472833.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":13.527,"RT..min.":7.6633,"First.Scan":5468,"Last.Scan":5468,"Master.Scan.s.":5464,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_02.raw","File.ID":"F26","Quan.Info":"","Peptides.Matched":2345,"XCorr":6.2,"X..Protein.Groups":1,"Percolator.q.Value":0.00022,"Percolator.PEP":1.582e-10,"Percolator.SVMScore":4.16,"Precursor.Area":6726867,"Apex.RT..min.":7.67,"ptmRS..Binomial.Peptide.Score":286.83,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(9): 1031.37; b1+-H(3) O(4) P(10): 1132.42; b1+-H(3) O(4) P(11): 1246.46; b2+-H(3) O(4) P(7): 394.65; b2+-H(3) O(4) P(8): 451.67; b2+-H(3) O(4) P(9): 516.19; b2+-H(3) O(4) P(13): 742.79; b2+-H(3) O(4) P(14): 799.81; b2+-H(3) O(4) P(15): 849.34; b2+-H(3) O(4) P(17): 954.41; b1+(4): 427.16","ptmRS..Best.Site.Probabilities":"S6(Phospho): 99.4","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(4): 0.6; Y(5): 0.0; S(6): 99.4; T(10): 0.0; T(13): 0.0; S(18): 0.0; S(19): 0.0; T(20): 0.0; T(23): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (K4)","Search.ID":"K","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FDDMELDENLLR","Annotated.Sequence":"[K].FDDmELDENLLR.[G]","Modifications":"M4(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.4542,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":763.34492,"Contaminant":"False","Aligned.m.z..Da.":763.34497,"MH...Da.":1525.68257,"Theo..MH...Da.":1525.6839,"DeltaM..ppm.":-0.87,"Deltam.z..Da.":-0.00067,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":626176.5625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":90.8504,"Ion.Inject.Time..ms.":22,"RT..min.":14.4697,"First.Scan":14146,"Last.Scan":14146,"Master.Scan.s.":14135,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_02.raw","File.ID":"F26","Quan.Info":"","Peptides.Matched":1153,"XCorr":2.62,"X..Protein.Groups":1,"Percolator.q.Value":0.00022,"Percolator.PEP":1.723e-07,"Percolator.SVMScore":2.779,"Precursor.Area":1650101.5,"Apex.RT..min.":14.49,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (K4)","Search.ID":"K","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"HSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[R].HSDsYsENETNHTNVPISSTGGTNNK.[T]","Modifications":"S4(Phospho); S6(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.1553,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":984.05598,"Contaminant":"False","Aligned.m.z..Da.":984.05618,"MH...Da.":2950.1534,"Theo..MH...Da.":2950.15241,"DeltaM..ppm.":0.34,"Deltam.z..Da.":0.00033,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2480439,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":8.0323,"First.Scan":5979,"Last.Scan":5979,"Master.Scan.s.":5971,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_02.raw","File.ID":"F26","Quan.Info":"","Peptides.Matched":1171,"XCorr":6.57,"X..Protein.Groups":1,"Percolator.q.Value":0.00022,"Percolator.PEP":3.01e-12,"Percolator.SVMScore":4.942,"Precursor.Area":6209161,"Apex.RT..min.":8.04,"ptmRS..Binomial.Peptide.Score":635.38,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(6): 739.21; y2+-H(3) O(4) P(23): 1257.03; y2+-H(3) O(4) P(22): 1173.53; y2+-H(3) O(4) P(21): 1092.00; b1+- -2H(3) O(4) P -2H(3) O(4) P(6): 641.23; b1+- -2H(3) O(4) P -2H(3) O(4) P(11): 1228.45; b1+- -2H(3) O(4) P -2H(3) O(4) P(15): 1679.67; b2+- -2H(3) O(4) P -2H(3) O(4) P(13): 733.78; b2+- -2H(3) O(4) P -2H(3) O(4) P(14): 790.80; b2+- -2H(3) O(4) P -2H(3) O(4) P(15): 840.34","ptmRS..Best.Site.Probabilities":"S4(Phospho): 100; S6(Phospho): 99.99","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(4): 100.0; Y(5): 0.0; S(6): 100.0; T(10): 0.0; T(13): 0.0; S(18): 0.0; S(19): 0.0; T(20): 0.0; T(23): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (K4)","Search.ID":"K","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.3355,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40099,"Contaminant":"False","Aligned.m.z..Da.":967.40096,"MH...Da.":2900.18844,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":-3.76,"Deltam.z..Da.":-0.00363,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":270370.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":14.985,"First.Scan":14803,"Last.Scan":14803,"Master.Scan.s.":14798,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_02.raw","File.ID":"F26","Quan.Info":"","Peptides.Matched":2147,"XCorr":4.56,"X..Protein.Groups":1,"Percolator.q.Value":0.00022,"Percolator.PEP":1.424e-08,"Percolator.SVMScore":3.271,"Precursor.Area":42622500,"Apex.RT..min.":15.09,"ptmRS..Binomial.Peptide.Score":344.79,"ptmRS..Isoform.Confidence.Probability":0.993,"ptmRS..Isoform.Group.Report":"y2+-H(3) O(4) P(14): 811.37","ptmRS..Best.Site.Probabilities":"S13(Phospho): 99.27","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.7; S(13): 99.3; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (K4)","Search.ID":"K","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FFISNPDLVDR","Annotated.Sequence":"[R].FFISNPDLVDR.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q03558","Master.Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","Protein.Accessions":"Q03558","Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.749,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":661.84059,"Contaminant":"False","Aligned.m.z..Da.":661.84061,"MH...Da.":1322.67391,"Theo..MH...Da.":1322.67393,"DeltaM..ppm.":-0.01,"Deltam.z..Da.":-1e-05,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":701990,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":22.53537,"Ion.Inject.Time..ms.":22,"RT..min.":14.6609,"First.Scan":14389,"Last.Scan":14389,"Master.Scan.s.":14382,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_02.raw","File.ID":"F26","Quan.Info":"","Peptides.Matched":632,"XCorr":2.55,"X..Protein.Groups":1,"Percolator.q.Value":0.00022,"Percolator.PEP":1.037e-06,"Percolator.SVMScore":2.424,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (K4)","Search.ID":"K","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"AAEAGETGAATSATEGDNNNNTAAGDKK","Annotated.Sequence":"[K].AAEAGETGAATsATEGDNNNNTAAGDKK.[G]","Modifications":"S12(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0076,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":906.04899,"Contaminant":"False","Aligned.m.z..Da.":906.04896,"MH...Da.":2716.13243,"Theo..MH...Da.":2716.13298,"DeltaM..ppm.":-0.2,"Deltam.z..Da.":-0.00018,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":6732860,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":2.86438,"Ion.Inject.Time..ms.":22,"RT..min.":4.9989,"First.Scan":1763,"Last.Scan":1763,"Master.Scan.s.":1759,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_02.raw","File.ID":"F26","Quan.Info":"","Peptides.Matched":2548,"XCorr":6.55,"X..Protein.Groups":1,"Percolator.q.Value":0.00022,"Percolator.PEP":6.305e-16,"Percolator.SVMScore":7.837,"ptmRS..Binomial.Peptide.Score":515.29,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(15): 1300.57; y1+-H(3) O(4) P(18): 1789.79; y1+-H(3) O(4) P(17): 1688.75; y2+-H(3) O(4) P(18): 895.40; y2+-H(3) O(4) P(17): 844.88; b1+(7): 630.27","ptmRS..Best.Site.Probabilities":"S12(Phospho): 99.99","ptmRS..Phospho.Site.Probabilities":"T(7): 0.0; T(11): 0.0; S(12): 100.0; T(14): 0.0; T(22): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (K4)","Search.ID":"K","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DIFSNDELLSDAYDAK","Annotated.Sequence":"[K].DIFSNDELLSDAYDAK.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":3,"DeltaScore":0.7463,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":908.41871,"Contaminant":"False","Aligned.m.z..Da.":908.41843,"MH...Da.":1815.83014,"Theo..MH...Da.":1815.82831,"DeltaM..ppm.":1,"Deltam.z..Da.":0.00091,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":5702532,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":1.09879,"Ion.Inject.Time..ms.":22,"RT..min.":17.0908,"First.Scan":17475,"Last.Scan":17475,"Master.Scan.s.":17473,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_02.raw","File.ID":"F26","Quan.Info":"","Peptides.Matched":1662,"XCorr":4.77,"X..Protein.Groups":1,"Percolator.q.Value":0.00022,"Percolator.PEP":2.05e-13,"Percolator.SVMScore":5.473,"Precursor.Area":3625942,"Apex.RT..min.":17.09,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (K4)","Search.ID":"K","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DIFSNDELLSDAYDAK","Annotated.Sequence":"[K].DIFSNDELLSDAYDAK.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6794,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":908.41961,"Contaminant":"False","Aligned.m.z..Da.":908.41934,"MH...Da.":1815.83195,"Theo..MH...Da.":1815.82831,"DeltaM..ppm.":2,"Deltam.z..Da.":0.00182,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1510172.625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":7.47664,"Ion.Inject.Time..ms.":22,"RT..min.":17.071,"First.Scan":17450,"Last.Scan":17450,"Master.Scan.s.":17447,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_02.raw","File.ID":"F26","Quan.Info":"","Peptides.Matched":1676,"XCorr":4.18,"X..Protein.Groups":1,"Percolator.q.Value":0.00022,"Percolator.PEP":4.396e-12,"Percolator.SVMScore":4.867,"Precursor.Area":3625942,"Apex.RT..min.":17.09,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (K4)","Search.ID":"K","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEySDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); Y12(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.4202,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40607,"Contaminant":"False","Aligned.m.z..Da.":967.40574,"MH...Da.":2900.20366,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":1.49,"Deltam.z..Da.":0.00144,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":238551.20312,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":17.3426,"First.Scan":17800,"Last.Scan":17800,"Master.Scan.s.":17798,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_02.raw","File.ID":"F26","Quan.Info":"","Peptides.Matched":2592,"XCorr":3.57,"X..Protein.Groups":1,"Percolator.q.Value":0.00022,"Percolator.PEP":4.794e-07,"Percolator.SVMScore":2.577,"ptmRS..Binomial.Peptide.Score":325.22,"ptmRS..Isoform.Confidence.Probability":0.007,"ptmRS..Isoform.Group.Report":"y2+-H(3) O(4) P(14): 811.37","ptmRS..Best.Site.Probabilities":"S13(Phospho): 99.29","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.7; S(13): 99.3; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (K4)","Search.ID":"K","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MFILDEADEMLSSGFK","Annotated.Sequence":"[K].mFILDEADEmLSSGFK.[E]","Modifications":"M1(Oxidation); M10(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6947,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":932.91737,"Contaminant":"False","Aligned.m.z..Da.":932.91716,"MH...Da.":1864.82748,"Theo..MH...Da.":1864.83433,"DeltaM..ppm.":-3.68,"Deltam.z..Da.":-0.00343,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":483824.0625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":5.56924,"Ion.Inject.Time..ms.":22,"RT..min.":16.6865,"First.Scan":16959,"Last.Scan":16959,"Master.Scan.s.":16953,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_02.raw","File.ID":"F26","Quan.Info":"","Peptides.Matched":1671,"XCorr":3.21,"X..Protein.Groups":1,"Percolator.q.Value":0.00022,"Percolator.PEP":1.954e-09,"Percolator.SVMScore":3.663,"Precursor.Area":695747,"Apex.RT..min.":16.71,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (K4)","Search.ID":"K","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"APQALMLAPTR","Annotated.Sequence":"[K].APQALMLAPTR.[E]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6401,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":584.82943,"Contaminant":"False","Aligned.m.z..Da.":584.82941,"MH...Da.":1168.65159,"Theo..MH...Da.":1168.65069,"DeltaM..ppm.":0.77,"Deltam.z..Da.":0.00045,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1031341.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":71.2653,"Ion.Inject.Time..ms.":22,"RT..min.":11.6148,"First.Scan":10413,"Last.Scan":10413,"Master.Scan.s.":10404,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_02.raw","File.ID":"F26","Quan.Info":"","Peptides.Matched":203,"XCorr":3.14,"X..Protein.Groups":1,"Percolator.q.Value":0.00022,"Percolator.PEP":9.571e-08,"Percolator.SVMScore":2.895,"Precursor.Area":877288.9375,"Apex.RT..min.":11.62,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (K4)","Search.ID":"K","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LQETNPEEVPKFEK","Annotated.Sequence":"[K].LQETNPEEVPKFEK.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.5939,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":563.28935,"Contaminant":"False","Aligned.m.z..Da.":563.28934,"MH...Da.":1687.85351,"Theo..MH...Da.":1687.85374,"DeltaM..ppm.":-0.14,"Deltam.z..Da.":-0.00008,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2270098.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":42.02548,"Ion.Inject.Time..ms.":22,"RT..min.":9.321,"First.Scan":7499,"Last.Scan":7499,"Master.Scan.s.":7492,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_02.raw","File.ID":"F26","Quan.Info":"","Peptides.Matched":925,"XCorr":2.29,"X..Protein.Groups":1,"Percolator.q.Value":0.00022,"Percolator.PEP":6.96e-07,"Percolator.SVMScore":2.503,"Precursor.Area":1754203.5,"Apex.RT..min.":9.32,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (K4)","Search.ID":"K","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"AGNFALHPEVVR","Annotated.Sequence":"[R].AGNFALHPEVVR.[E]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q03558","Master.Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","Protein.Accessions":"Q03558","Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.6987,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":437.23758,"Contaminant":"False","Aligned.m.z..Da.":437.23756,"MH...Da.":1309.6982,"Theo..MH...Da.":1309.70114,"DeltaM..ppm.":-2.25,"Deltam.z..Da.":-0.00098,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2718458,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":9.52888,"Ion.Inject.Time..ms.":22,"RT..min.":10.0491,"First.Scan":8373,"Last.Scan":8373,"Master.Scan.s.":8363,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_02.raw","File.ID":"F26","Quan.Info":"","Peptides.Matched":452,"XCorr":3.02,"X..Protein.Groups":1,"Percolator.q.Value":0.00022,"Percolator.PEP":2.609e-08,"Percolator.SVMScore":3.152,"Precursor.Area":1535504.625,"Apex.RT..min.":10.04,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (K4)","Search.ID":"K","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"APQALMLAPTR","Annotated.Sequence":"[K].APQALmLAPTR.[E]","Modifications":"M6(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7579,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":592.82658,"Contaminant":"False","Aligned.m.z..Da.":592.82656,"MH...Da.":1184.64589,"Theo..MH...Da.":1184.6456,"DeltaM..ppm.":0.24,"Deltam.z..Da.":0.00014,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2151056,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":25.16548,"Ion.Inject.Time..ms.":22,"RT..min.":10.1735,"First.Scan":8525,"Last.Scan":8525,"Master.Scan.s.":8519,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_02.raw","File.ID":"F26","Quan.Info":"","Peptides.Matched":304,"XCorr":3.18,"X..Protein.Groups":1,"Percolator.q.Value":0.00022,"Percolator.PEP":3.19e-07,"Percolator.SVMScore":2.657,"Precursor.Area":3356030.5,"Apex.RT..min.":10.2,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (K4)","Search.ID":"K","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KDELTLEGIK","Annotated.Sequence":"[K].KDELTLEGIK.[Q]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5179,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":573.32444,"Contaminant":"False","Aligned.m.z..Da.":573.32442,"MH...Da.":1145.64161,"Theo..MH...Da.":1145.64123,"DeltaM..ppm.":0.33,"Deltam.z..Da.":0.00019,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":4257306.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":1.40552,"Ion.Inject.Time..ms.":22,"RT..min.":10.2562,"First.Scan":8626,"Last.Scan":8626,"Master.Scan.s.":8623,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_02.raw","File.ID":"F26","Quan.Info":"","Peptides.Matched":307,"XCorr":3.9,"X..Protein.Groups":1,"Percolator.q.Value":0.00022,"Percolator.PEP":7.092e-09,"Percolator.SVMScore":3.409,"Precursor.Area":3685926.75,"Apex.RT..min.":10.26,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (K4)","Search.ID":"K","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"AIMPIIEGHDVLAQAQSGTGK","Annotated.Sequence":"[R].AImPIIEGHDVLAQAQSGTGK.[T]","Modifications":"M3(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.7094,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":718.04041,"Contaminant":"False","Aligned.m.z..Da.":718.04047,"MH...Da.":2152.10667,"Theo..MH...Da.":2152.10668,"DeltaM..ppm.":0,"Deltam.z..Da.":0,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":803632.625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":8.44958,"Ion.Inject.Time..ms.":22,"RT..min.":12.7589,"First.Scan":12005,"Last.Scan":12005,"Master.Scan.s.":12003,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_02.raw","File.ID":"F26","Quan.Info":"","Peptides.Matched":989,"XCorr":4.68,"X..Protein.Groups":1,"Percolator.q.Value":0.00022,"Percolator.PEP":2.029e-12,"Percolator.SVMScore":5.02,"Precursor.Area":1523420.375,"Apex.RT..min.":12.78,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (K4)","Search.ID":"K","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MGSINNNFNTNNNSNTDLDRDWK","Annotated.Sequence":"[-].mGSINNNFNTNNNsNTDLDRDWK.[T]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S14(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P39517","Master.Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","Protein.Accessions":"P39517","Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.057,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1338.05537,"Contaminant":"False","Aligned.m.z..Da.":1338.05561,"MH...Da.":2675.10346,"Theo..MH...Da.":2675.11179,"DeltaM..ppm.":-3.11,"Deltam.z..Da.":-0.00416,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1173322.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":13.5577,"First.Scan":13009,"Last.Scan":13009,"Master.Scan.s.":13004,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_02.raw","File.ID":"F26","Quan.Info":"","Peptides.Matched":2087,"XCorr":4.74,"X..Protein.Groups":1,"Percolator.q.Value":0.00022,"Percolator.PEP":9.054e-11,"Percolator.SVMScore":4.27,"Precursor.Area":743609.375,"Apex.RT..min.":13.56,"ptmRS..Binomial.Peptide.Score":697.94,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(16): 1935.86; y1+-H(3) O(4) P(15): 1788.79; y1+-H(3) O(4) P(14): 1674.75; y1+-H(3) O(4) P(13): 1573.70; y1+-H(3) O(4) P(12): 1459.66; y1+-H(3) O(4) P(11): 1345.61; y1+-H(3) O(4) P(10): 1231.57; b2+-H(3) O(4) P(19): 987.41; b2+-H(3) O(4) P(21): 1122.98; y2+-H(3) O(4) P(19): 1139.50; y2+-H(3) O(4) P(17): 1025.45; y1+(9): 1162.55; y1+(8): 1048.51","ptmRS..Best.Site.Probabilities":"S14(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(3): 0.0; T(10): 0.0; S(14): 100.0; T(16): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (K4)","Search.ID":"K","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DFLLKPELSR","Annotated.Sequence":"[K].DFLLKPELSR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.6123,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":406.56796,"Contaminant":"False","Aligned.m.z..Da.":406.56802,"MH...Da.":1217.68934,"Theo..MH...Da.":1217.68885,"DeltaM..ppm.":0.4,"Deltam.z..Da.":0.00016,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":791425,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":4.28876,"Ion.Inject.Time..ms.":22,"RT..min.":13.148,"First.Scan":12504,"Last.Scan":12504,"Master.Scan.s.":12497,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_02.raw","File.ID":"F26","Quan.Info":"","Peptides.Matched":265,"XCorr":2.76,"X..Protein.Groups":1,"Percolator.q.Value":0.00022,"Percolator.PEP":2.746e-07,"Percolator.SVMScore":2.687,"Precursor.Area":635858.1875,"Apex.RT..min.":13.15,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (K4)","Search.ID":"K","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"TGTFSIAALQR","Annotated.Sequence":"[K].TGTFSIAALQR.[I]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7781,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":582.82198,"Contaminant":"False","Aligned.m.z..Da.":582.82208,"MH...Da.":1164.63668,"Theo..MH...Da.":1164.63715,"DeltaM..ppm.":-0.4,"Deltam.z..Da.":-0.00023,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1413752,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":5.27103,"Ion.Inject.Time..ms.":22,"RT..min.":13.3458,"First.Scan":12748,"Last.Scan":12748,"Master.Scan.s.":12744,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_02.raw","File.ID":"F26","Quan.Info":"","Peptides.Matched":364,"XCorr":3.74,"X..Protein.Groups":1,"Percolator.q.Value":0.00022,"Percolator.PEP":2.043e-07,"Percolator.SVMScore":2.745,"Precursor.Area":2585077.25,"Apex.RT..min.":13.36,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (I4)","Search.ID":"I","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"TGTFSIAALQR","Annotated.Sequence":"[K].TGTFSIAALQR.[I]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7462,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":582.82208,"Contaminant":"False","Aligned.m.z..Da.":582.82207,"MH...Da.":1164.63689,"Theo..MH...Da.":1164.63715,"DeltaM..ppm.":-0.22,"Deltam.z..Da.":-0.00013,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1522301.375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":14.30954,"Ion.Inject.Time..ms.":22,"RT..min.":13.3556,"First.Scan":12791,"Last.Scan":12791,"Master.Scan.s.":12788,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_04.raw","File.ID":"F28","Quan.Info":"","Peptides.Matched":364,"XCorr":3.31,"X..Protein.Groups":1,"Percolator.q.Value":0.00023,"Percolator.PEP":0.00006,"Percolator.SVMScore":2.259,"Precursor.Area":2631902,"Apex.RT..min.":13.37,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (I4)","Search.ID":"I","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0018,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":953.40444,"Contaminant":"False","Aligned.m.z..Da.":953.40443,"MH...Da.":2858.19879,"Theo..MH...Da.":2858.18876,"DeltaM..ppm.":3.51,"Deltam.z..Da.":0.00334,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1070014.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":30.6795,"Ion.Inject.Time..ms.":22,"RT..min.":13.8329,"First.Scan":13400,"Last.Scan":13400,"Master.Scan.s.":13399,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_04.raw","File.ID":"F28","Quan.Info":"","Peptides.Matched":2621,"XCorr":5.63,"X..Protein.Groups":1,"Percolator.q.Value":0.00023,"Percolator.PEP":9.27e-07,"Percolator.SVMScore":3.785,"ptmRS..Binomial.Peptide.Score":445.01,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(15): 1750.78; y1+-H(3) O(4) P(14): 1621.73; y1+-H(3) O(4) P(13): 1458.67; b2+-H(3) O(4) P(18): 993.90; b2+-H(3) O(4) P(21): 1171.01; y2+-H(3) O(4) P(14): 811.37; b1+(12): 1302.55","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (I4)","Search.ID":"I","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEySDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss); Y12(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":4,"DeltaScore":0.0101,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":953.4003,"Contaminant":"False","Aligned.m.z..Da.":953.40028,"MH...Da.":2858.18634,"Theo..MH...Da.":2858.18876,"DeltaM..ppm.":-0.85,"Deltam.z..Da.":-0.00081,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":921843,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":71.15585,"Ion.Inject.Time..ms.":22,"RT..min.":13.8276,"First.Scan":13395,"Last.Scan":13395,"Master.Scan.s.":13386,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_04.raw","File.ID":"F28","Quan.Info":"","Peptides.Matched":2357,"XCorr":3.95,"X..Protein.Groups":1,"Percolator.q.Value":0.00023,"Percolator.PEP":0.00001,"Percolator.SVMScore":2.91,"ptmRS..Binomial.Peptide.Score":243.1,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(14): 1621.73; b2+-H(3) O(4) P(21): 1171.01; y2+-H(3) O(4) P(14): 811.37; b1+(12): 1302.55","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (I4)","Search.ID":"I","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].mSEGITDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7695,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1056.48638,"Contaminant":"False","Aligned.m.z..Da.":1056.4864,"MH...Da.":2111.96549,"Theo..MH...Da.":2111.96151,"DeltaM..ppm.":1.88,"Deltam.z..Da.":0.00199,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":480616.4375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":19.05321,"Ion.Inject.Time..ms.":22,"RT..min.":14.6456,"First.Scan":14435,"Last.Scan":14435,"Master.Scan.s.":14426,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_04.raw","File.ID":"F28","Quan.Info":"","Peptides.Matched":2286,"XCorr":4.99,"X..Protein.Groups":1,"Percolator.q.Value":0.00023,"Percolator.PEP":3.592e-07,"Percolator.SVMScore":4.124,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (I4)","Search.ID":"I","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"GVFGYGFEEPSAIQQR","Annotated.Sequence":"[R].GVFGYGFEEPSAIQQR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7493,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":892.93313,"Contaminant":"False","Aligned.m.z..Da.":892.93315,"MH...Da.":1784.85899,"Theo..MH...Da.":1784.86022,"DeltaM..ppm.":-0.69,"Deltam.z..Da.":-0.00062,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":558849.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":39.41919,"Ion.Inject.Time..ms.":22,"RT..min.":15.0145,"First.Scan":14899,"Last.Scan":14899,"Master.Scan.s.":14894,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_04.raw","File.ID":"F28","Quan.Info":"","Peptides.Matched":1284,"XCorr":3.83,"X..Protein.Groups":1,"Percolator.q.Value":0.00023,"Percolator.PEP":1.589e-06,"Percolator.SVMScore":3.593,"Precursor.Area":1478444.875,"Apex.RT..min.":15.04,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (I4)","Search.ID":"I","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FTVSAIYSDLPQQER","Annotated.Sequence":"[K].FTVSAIYSDLPQQER.[D]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7021,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":877.44297,"Contaminant":"False","Aligned.m.z..Da.":877.44299,"MH...Da.":1753.87866,"Theo..MH...Da.":1753.87554,"DeltaM..ppm.":1.78,"Deltam.z..Da.":0.00156,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":646865.4375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":11.10694,"Ion.Inject.Time..ms.":22,"RT..min.":15.0253,"First.Scan":14913,"Last.Scan":14913,"Master.Scan.s.":14907,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_04.raw","File.ID":"F28","Quan.Info":"","Peptides.Matched":1115,"XCorr":3.86,"X..Protein.Groups":1,"Percolator.q.Value":0.00023,"Percolator.PEP":2.395e-06,"Percolator.SVMScore":3.446,"Precursor.Area":1119738.75,"Apex.RT..min.":15.05,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (I4)","Search.ID":"I","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KDELTLEGIK","Annotated.Sequence":"[K].KDELTLEGIK.[Q]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5359,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":573.32394,"Contaminant":"False","Aligned.m.z..Da.":573.32389,"MH...Da.":1145.64061,"Theo..MH...Da.":1145.64123,"DeltaM..ppm.":-0.54,"Deltam.z..Da.":-0.00031,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2568619.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":11.26641,"Ion.Inject.Time..ms.":22,"RT..min.":10.2463,"First.Scan":8647,"Last.Scan":8647,"Master.Scan.s.":8641,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_04.raw","File.ID":"F28","Quan.Info":"","Peptides.Matched":314,"XCorr":3.9,"X..Protein.Groups":1,"Percolator.q.Value":0.00023,"Percolator.PEP":6.555e-06,"Percolator.SVMScore":3.086,"Precursor.Area":4063837,"Apex.RT..min.":10.26,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (I4)","Search.ID":"I","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"AIMPIIEGHDVLAQAQSGTGK","Annotated.Sequence":"[R].AImPIIEGHDVLAQAQSGTGK.[T]","Modifications":"M3(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.6943,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":718.0408,"Contaminant":"False","Aligned.m.z..Da.":718.04078,"MH...Da.":2152.10784,"Theo..MH...Da.":2152.10668,"DeltaM..ppm.":0.54,"Deltam.z..Da.":0.00039,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":795629.1875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":7.72252,"Ion.Inject.Time..ms.":22,"RT..min.":12.7558,"First.Scan":12017,"Last.Scan":12017,"Master.Scan.s.":12008,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_04.raw","File.ID":"F28","Quan.Info":"","Peptides.Matched":985,"XCorr":5.07,"X..Protein.Groups":1,"Percolator.q.Value":0.00023,"Percolator.PEP":4.38e-07,"Percolator.SVMScore":4.053,"Precursor.Area":1399497,"Apex.RT..min.":12.77,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (I4)","Search.ID":"I","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"APQALMLAPTR","Annotated.Sequence":"[K].APQALMLAPTR.[E]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6497,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":584.82965,"Contaminant":"False","Aligned.m.z..Da.":584.82964,"MH...Da.":1168.65204,"Theo..MH...Da.":1168.65069,"DeltaM..ppm.":1.15,"Deltam.z..Da.":0.00067,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":953403.4375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":73.02573,"Ion.Inject.Time..ms.":22,"RT..min.":11.6062,"First.Scan":10455,"Last.Scan":10455,"Master.Scan.s.":10448,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_04.raw","File.ID":"F28","Quan.Info":"","Peptides.Matched":202,"XCorr":2.94,"X..Protein.Groups":1,"Percolator.q.Value":0.00023,"Percolator.PEP":0.00009,"Percolator.SVMScore":2.121,"Precursor.Area":905291.5625,"Apex.RT..min.":11.62,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (I4)","Search.ID":"I","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"LTESTVNHTNHSDDELPGHLLLDSGASR","Annotated.Sequence":"[K].LTEsTVNHTNHSDDELPGHLLLDSGASR.[T]","Modifications":"S4(Phospho)","X..Proteins":15,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"Q99337; P0C2I9; P47100; P0C2J0; Q03619; P0C2I2; Q99231; Q12088; Q07793; Q04711; Q12316; O13535; Q04670; Q92393; Q12193","Protein.Descriptions":"Transposon Ty1-NL2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-NL2 PE=3 SV=1; Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-ER2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER2 PE=3 SV=1; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-LR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR1 PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-GR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR3 PE=3 SV=3; Transposon Ty1-H Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-H PE=3 SV=1; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-BR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-BR PE=3 SV=1","X..Missed.Cleavages":0,"Charge":4,"Original.Precursor.Charge":4,"DeltaScore":0.0028,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":774.60674,"Contaminant":"False","Aligned.m.z..Da.":774.60672,"MH...Da.":3095.40512,"Theo..MH...Da.":3095.40658,"DeltaM..ppm.":-0.47,"Deltam.z..Da.":-0.00036,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":306209.0625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":27.07602,"Ion.Inject.Time..ms.":22,"RT..min.":12.2567,"First.Scan":11349,"Last.Scan":11349,"Master.Scan.s.":11345,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_04.raw","File.ID":"F28","Quan.Info":"","Peptides.Matched":4594,"XCorr":3.55,"X..Protein.Groups":1,"Percolator.q.Value":0.00023,"Percolator.PEP":0.0001,"Percolator.SVMScore":2.098,"Precursor.Area":802034.875,"Apex.RT..min.":12.28,"ptmRS..Binomial.Peptide.Score":209.6,"ptmRS..Isoform.Confidence.Probability":0.33,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(2): 197.13; b2+-H(3) O(4) P(18): 965.43","ptmRS..Best.Site.Probabilities":"T2(Phospho): 33.04; S4(Phospho): 33.04; T5(Phospho): 33.04","ptmRS..Phospho.Site.Probabilities":"T(2): 33.0; S(4): 33.0; T(5): 33.0; T(9): 0.4; S(12): 0.4; S(24): 0.0; S(27): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (I4)","Search.ID":"I","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MGSINNNFNTNNNSNTDLDRDWK","Annotated.Sequence":"[-].mGSINNNFNTNNNsNTDLDRDWK.[T]","Modifications":"N-Term(Prot)(Met-loss); S14(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P39517","Master.Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","Protein.Accessions":"P39517","Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0763,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":878.36989,"Contaminant":"False","Aligned.m.z..Da.":878.36985,"MH...Da.":2633.09512,"Theo..MH...Da.":2633.10123,"DeltaM..ppm.":-2.32,"Deltam.z..Da.":-0.00204,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3641647.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":52.91454,"Ion.Inject.Time..ms.":22,"RT..min.":11.3101,"First.Scan":10048,"Last.Scan":10048,"Master.Scan.s.":10045,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_04.raw","File.ID":"F28","Quan.Info":"","Peptides.Matched":2131,"XCorr":6.16,"X..Protein.Groups":1,"Percolator.q.Value":0.00023,"Percolator.PEP":4.691e-08,"Percolator.SVMScore":4.852,"Precursor.Area":4151848,"Apex.RT..min.":11.32,"ptmRS..Binomial.Peptide.Score":645.91,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(14): 1674.75; y1+-H(3) O(4) P(12): 1459.66; y1+-H(3) O(4) P(11): 1345.61; y1+-H(3) O(4) P(10): 1231.57; y2+-H(3) O(4) P(19): 1139.50; y2+-H(3) O(4) P(18): 1082.48; y2+-H(3) O(4) P(17): 1025.45; y2+-H(3) O(4) P(16): 968.43; y2+-H(3) O(4) P(15): 894.90; y2+-H(3) O(4) P(14): 837.88; y2+-H(3) O(4) P(13): 787.35; y2+-H(3) O(4) P(12): 730.33; y2+-H(3) O(4) P(11): 673.31; y2+-H(3) O(4) P(10): 616.29; y1+(9): 1162.55; y1+(8): 1048.51","ptmRS..Best.Site.Probabilities":"S14(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(3): 0.0; T(10): 0.0; S(14): 100.0; T(16): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (I4)","Search.ID":"I","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DIFSNDELLSDAYDAK","Annotated.Sequence":"[K].DIFSNDELLSDAYDAK.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.7283,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":605.94815,"Contaminant":"False","Aligned.m.z..Da.":605.94803,"MH...Da.":1815.8299,"Theo..MH...Da.":1815.82831,"DeltaM..ppm.":0.87,"Deltam.z..Da.":0.00053,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":732959.4375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":12.90162,"Ion.Inject.Time..ms.":22,"RT..min.":17.0881,"First.Scan":17524,"Last.Scan":17524,"Master.Scan.s.":17520,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_04.raw","File.ID":"F28","Quan.Info":"","Peptides.Matched":1618,"XCorr":2.76,"X..Protein.Groups":1,"Percolator.q.Value":0.00023,"Percolator.PEP":0.00003,"Percolator.SVMScore":2.58,"Precursor.Area":819916.6875,"Apex.RT..min.":17.1,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (I4)","Search.ID":"I","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DIFSNDELLSDAYDAK","Annotated.Sequence":"[K].DIFSNDELLSDAYDAK.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6676,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":908.41852,"Contaminant":"False","Aligned.m.z..Da.":908.41834,"MH...Da.":1815.82977,"Theo..MH...Da.":1815.82831,"DeltaM..ppm.":0.8,"Deltam.z..Da.":0.00073,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":698931.3125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":12.3221,"Ion.Inject.Time..ms.":22,"RT..min.":17.0775,"First.Scan":17510,"Last.Scan":17510,"Master.Scan.s.":17507,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_04.raw","File.ID":"F28","Quan.Info":"","Peptides.Matched":1573,"XCorr":3.61,"X..Protein.Groups":1,"Percolator.q.Value":0.00023,"Percolator.PEP":1.017e-06,"Percolator.SVMScore":3.752,"Precursor.Area":3683971.75,"Apex.RT..min.":17.1,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (I4)","Search.ID":"I","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MFILDEADEMLSSGFK","Annotated.Sequence":"[K].mFILDEADEmLSSGFK.[E]","Modifications":"M1(Oxidation); M10(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7155,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":932.91861,"Contaminant":"False","Aligned.m.z..Da.":932.91853,"MH...Da.":1864.82995,"Theo..MH...Da.":1864.83433,"DeltaM..ppm.":-2.35,"Deltam.z..Da.":-0.00219,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":659460.8125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":16.686,"First.Scan":17008,"Last.Scan":17008,"Master.Scan.s.":17000,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_04.raw","File.ID":"F28","Quan.Info":"","Peptides.Matched":1823,"XCorr":3.41,"X..Protein.Groups":1,"Percolator.q.Value":0.00023,"Percolator.PEP":0.00001,"Percolator.SVMScore":2.895,"Precursor.Area":645336.5625,"Apex.RT..min.":16.7,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (I4)","Search.ID":"I","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"HSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[R].HSDsYsENETNHTNVPISSTGGTNNK.[T]","Modifications":"S4(Phospho); S6(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":0,"Charge":4,"Original.Precursor.Charge":4,"DeltaScore":0.092,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":738.29352,"Contaminant":"False","Aligned.m.z..Da.":738.2936,"MH...Da.":2950.15226,"Theo..MH...Da.":2950.15241,"DeltaM..ppm.":-0.05,"Deltam.z..Da.":-0.00004,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3398778.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":73.65778,"Ion.Inject.Time..ms.":22,"RT..min.":8.0463,"First.Scan":6012,"Last.Scan":6012,"Master.Scan.s.":6002,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_04.raw","File.ID":"F28","Quan.Info":"","Peptides.Matched":1116,"XCorr":4.35,"X..Protein.Groups":1,"Percolator.q.Value":0.00023,"Percolator.PEP":0.0001,"Percolator.SVMScore":2.077,"ptmRS..Binomial.Peptide.Score":263.64,"ptmRS..Isoform.Confidence.Probability":0.012,"ptmRS..Isoform.Group.Report":"b2+-H(3) O(4) P(14): 839.79; b2+-H(3) O(4) P(15): 889.33; b2+-H(3) O(4) P(17): 994.39; y2+- -2H(3) O(4) P -2H(3) O(4) P(20): 1039.48","ptmRS..Best.Site.Probabilities":"T10(Phospho): 92.95; T13(Phospho): 92.95","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(4): 4.7; Y(5): 4.7; S(6): 4.7; T(10): 93.0; T(13): 93.0; S(18): 0.0; S(19): 0.0; T(20): 0.0; T(23): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (I4)","Search.ID":"I","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"AAEAGETGAATSATEGDNNNNTAAGDK","Annotated.Sequence":"[K].AAEAGETGAATsATEGDNNNNTAAGDK.[K]","Modifications":"S12(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0038,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":863.35031,"Contaminant":"False","Aligned.m.z..Da.":863.35033,"MH...Da.":2588.03638,"Theo..MH...Da.":2588.03802,"DeltaM..ppm.":-0.63,"Deltam.z..Da.":-0.00055,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2548607,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":5.7924,"First.Scan":2604,"Last.Scan":2604,"Master.Scan.s.":2596,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_04.raw","File.ID":"F28","Quan.Info":"","Peptides.Matched":1505,"XCorr":5.2,"X..Protein.Groups":1,"Percolator.q.Value":0.00023,"Percolator.PEP":1.896e-07,"Percolator.SVMScore":4.353,"ptmRS..Binomial.Peptide.Score":376.06,"ptmRS..Isoform.Confidence.Probability":0.496,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(13): 1070.47","ptmRS..Best.Site.Probabilities":"T11(Phospho): 49.63; S12(Phospho): 49.63","ptmRS..Phospho.Site.Probabilities":"T(7): 0.4; T(11): 49.6; S(12): 49.6; T(14): 0.4; T(22): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (I4)","Search.ID":"I","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"HSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[R].HSDsYsENETNHTNVPISSTGGTNNK.[T]","Modifications":"S4(Phospho); S6(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.1382,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":984.05565,"Contaminant":"False","Aligned.m.z..Da.":984.05576,"MH...Da.":2950.15241,"Theo..MH...Da.":2950.15241,"DeltaM..ppm.":0,"Deltam.z..Da.":0,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":6308233,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":8.0312,"First.Scan":5992,"Last.Scan":5992,"Master.Scan.s.":5989,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_04.raw","File.ID":"F28","Quan.Info":"","Peptides.Matched":1118,"XCorr":7.02,"X..Protein.Groups":1,"Percolator.q.Value":0.00023,"Percolator.PEP":1.383e-06,"Percolator.SVMScore":3.642,"Precursor.Area":6223342,"Apex.RT..min.":8.04,"ptmRS..Binomial.Peptide.Score":637.44,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(4): 409.15; b1+-H(3) O(4) P(5): 572.21; b1+-H(3) O(4) P(6): 739.21; b1+-H(3) O(4) P(9): 1111.34; b1+-H(3) O(4) P(11): 1326.43; b1+-H(3) O(4) P(15): 1777.64; b2+-H(3) O(4) P(12): 732.25; b2+-H(3) O(4) P(14): 839.79; b2+-H(3) O(4) P(15): 889.33; y2+-H(3) O(4) P(23): 1257.03; y2+- -2H(3) O(4) P -2H(3) O(4) P(24): 1265.56; b1+(2): 225.10; b1+(3): 340.13","ptmRS..Best.Site.Probabilities":"S4(Phospho): 100; S6(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(4): 100.0; Y(5): 0.0; S(6): 100.0; T(10): 0.0; T(13): 0.0; S(18): 0.0; S(19): 0.0; T(20): 0.0; T(23): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (I4)","Search.ID":"I","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LQETNPEEVPK","Annotated.Sequence":"[K].LQETNPEEVPK.[F]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5207,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":642.328,"Contaminant":"False","Aligned.m.z..Da.":642.32804,"MH...Da.":1283.64873,"Theo..MH...Da.":1283.64777,"DeltaM..ppm.":0.75,"Deltam.z..Da.":0.00048,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3000253,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":10.96866,"Ion.Inject.Time..ms.":22,"RT..min.":7.6359,"First.Scan":5476,"Last.Scan":5476,"Master.Scan.s.":5469,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_04.raw","File.ID":"F28","Quan.Info":"","Peptides.Matched":547,"XCorr":2.9,"X..Protein.Groups":1,"Percolator.q.Value":0.00023,"Percolator.PEP":0.00004,"Percolator.SVMScore":2.42,"Precursor.Area":9414349,"Apex.RT..min.":7.65,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (I4)","Search.ID":"I","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0515,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40535,"Contaminant":"False","Aligned.m.z..Da.":967.40537,"MH...Da.":2900.20152,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":0.76,"Deltam.z..Da.":0.00073,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":845403.375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":14.12798,"Ion.Inject.Time..ms.":22,"RT..min.":15.5625,"First.Scan":15584,"Last.Scan":15584,"Master.Scan.s.":15583,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_04.raw","File.ID":"F28","Quan.Info":"","Peptides.Matched":2519,"XCorr":4.85,"X..Protein.Groups":1,"Percolator.q.Value":0.00023,"Percolator.PEP":1.719e-06,"Percolator.SVMScore":3.564,"Precursor.Area":54447288,"Apex.RT..min.":15.1,"ptmRS..Binomial.Peptide.Score":379,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(13): 1413.58; b1+(12): 1344.56","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (I4)","Search.ID":"I","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"AAEAGETGAATSATEGDNNNNTAAGDKK","Annotated.Sequence":"[K].AAEAGETGAATSATEGDNNNNTAAGDKK.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.726,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":879.39439,"Contaminant":"False","Aligned.m.z..Da.":879.39437,"MH...Da.":2636.16863,"Theo..MH...Da.":2636.16665,"DeltaM..ppm.":0.75,"Deltam.z..Da.":0.00066,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1418460.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":4.9176,"First.Scan":1658,"Last.Scan":1658,"Master.Scan.s.":1647,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_04.raw","File.ID":"F28","Quan.Info":"","Peptides.Matched":3467,"XCorr":8.14,"X..Protein.Groups":1,"Percolator.q.Value":0.00023,"Percolator.PEP":4.411e-12,"Percolator.SVMScore":8.166,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (I4)","Search.ID":"I","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].mSEGItDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); T6(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.1714,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":731.31571,"Contaminant":"False","Aligned.m.z..Da.":731.31572,"MH...Da.":2191.93258,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":2.16,"Deltam.z..Da.":0.00158,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":601106.5625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":6.32608,"Ion.Inject.Time..ms.":22,"RT..min.":15.5351,"First.Scan":15551,"Last.Scan":15551,"Master.Scan.s.":15544,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_04.raw","File.ID":"F28","Quan.Info":"","Peptides.Matched":2120,"XCorr":4.2,"X..Protein.Groups":1,"Percolator.q.Value":0.00023,"Percolator.PEP":0.00003,"Percolator.SVMScore":2.528,"Precursor.Area":687694.375,"Apex.RT..min.":15.55,"ptmRS..Binomial.Peptide.Score":399.56,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(6): 512.24; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+-H(3) O(4) P(9): 869.39; b1+-H(3) O(4) P(10): 998.43; y2+-H(3) O(4) P(14): 833.38; b1+(2): 130.05; b1+(3): 259.09; b1+(4): 316.11; b1+(5): 429.20; b2+(3): 130.05","ptmRS..Best.Site.Probabilities":"T6(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; T(6): 100.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (I4)","Search.ID":"I","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FFISNPDLVDRLEK","Annotated.Sequence":"[R].FFISNPDLVDRLEK.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q03558","Master.Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","Protein.Accessions":"Q03558","Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.7669,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":564.96952,"Contaminant":"False","Aligned.m.z..Da.":564.96953,"MH...Da.":1692.894,"Theo..MH...Da.":1692.89555,"DeltaM..ppm.":-0.91,"Deltam.z..Da.":-0.00051,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":862086.625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":48.94717,"Ion.Inject.Time..ms.":22,"RT..min.":15.3459,"First.Scan":15314,"Last.Scan":15314,"Master.Scan.s.":15310,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_04.raw","File.ID":"F28","Quan.Info":"","Peptides.Matched":538,"XCorr":2.66,"X..Protein.Groups":1,"Percolator.q.Value":0.00023,"Percolator.PEP":0.00004,"Percolator.SVMScore":2.477,"Precursor.Area":495093.5625,"Apex.RT..min.":15.35,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (I4)","Search.ID":"I","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"HSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[R].HSDSySENETNHTNVPISSTGGTNNK.[T]","Modifications":"Y5(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":0,"Charge":4,"Original.Precursor.Charge":4,"DeltaScore":0.005,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":718.30166,"Contaminant":"False","Aligned.m.z..Da.":718.3017,"MH...Da.":2870.1848,"Theo..MH...Da.":2870.18608,"DeltaM..ppm.":-0.45,"Deltam.z..Da.":-0.00032,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":4899633.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":1.3305,"Ion.Inject.Time..ms.":22,"RT..min.":7.6531,"First.Scan":5499,"Last.Scan":5499,"Master.Scan.s.":5495,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_04.raw","File.ID":"F28","Quan.Info":"","Peptides.Matched":2460,"XCorr":6.04,"X..Protein.Groups":1,"Percolator.q.Value":0.00023,"Percolator.PEP":2.508e-07,"Percolator.SVMScore":4.253,"Precursor.Area":6787289.5,"Apex.RT..min.":7.66,"ptmRS..Binomial.Peptide.Score":302.19,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(9): 1031.37; b1+-H(3) O(4) P(11): 1246.46; b1+-H(3) O(4) P(12): 1383.52; b1+-H(3) O(4) P(15): 1697.68; b2+-H(3) O(4) P(7): 394.65; b2+-H(3) O(4) P(13): 742.79; b2+-H(3) O(4) P(14): 799.81; b2+-H(3) O(4) P(15): 849.34; b2+-H(3) O(4) P(17): 954.41; b1+(4): 427.16","ptmRS..Best.Site.Probabilities":"S6(Phospho): 99.36","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(4): 0.6; Y(5): 0.0; S(6): 99.4; T(10): 0.0; T(13): 0.0; S(18): 0.0; S(19): 0.0; T(20): 0.0; T(23): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (I4)","Search.ID":"I","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"HSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[R].HSDSySENETNHTNVPISSTGGTNNK.[T]","Modifications":"Y5(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0045,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":957.39966,"Contaminant":"False","Aligned.m.z..Da.":957.39972,"MH...Da.":2870.18445,"Theo..MH...Da.":2870.18608,"DeltaM..ppm.":-0.57,"Deltam.z..Da.":-0.00054,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":8378850,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":11.729,"RT..min.":7.6525,"First.Scan":5498,"Last.Scan":5498,"Master.Scan.s.":5495,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_04.raw","File.ID":"F28","Quan.Info":"","Peptides.Matched":2473,"XCorr":6.72,"X..Protein.Groups":1,"Percolator.q.Value":0.00023,"Percolator.PEP":8.717e-10,"Percolator.SVMScore":6.276,"Precursor.Area":12732608,"Apex.RT..min.":7.66,"ptmRS..Binomial.Peptide.Score":308.66,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(6): 659.24; b1+-H(3) O(4) P(9): 1031.37; b1+-H(3) O(4) P(11): 1246.46; b1+-H(3) O(4) P(12): 1383.52; b1+-H(3) O(4) P(15): 1697.68; b2+-H(3) O(4) P(9): 516.19; b2+-H(3) O(4) P(12): 692.26; b2+-H(3) O(4) P(13): 742.79; b2+-H(3) O(4) P(14): 799.81; b2+-H(3) O(4) P(15): 849.34; y2+-H(3) O(4) P(25): 1318.08; y2+-H(3) O(4) P(24): 1274.56; y2+-H(3) O(4) P(23): 1217.05; y2+-H(3) O(4) P(21): 1092.00; b1+(4): 427.16","ptmRS..Best.Site.Probabilities":"S6(Phospho): 99.41","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(4): 0.6; Y(5): 0.0; S(6): 99.4; T(10): 0.0; T(13): 0.0; S(18): 0.0; S(19): 0.0; T(20): 0.0; T(23): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (I4)","Search.ID":"I","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].mSEGItDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); T6(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1008,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1096.47238,"Contaminant":"False","Aligned.m.z..Da.":1096.4724,"MH...Da.":2191.93748,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":4.4,"Deltam.z..Da.":0.00482,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":446670.15625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":15.5274,"First.Scan":15542,"Last.Scan":15542,"Master.Scan.s.":15531,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_04.raw","File.ID":"F28","Quan.Info":"","Peptides.Matched":2167,"XCorr":4.86,"X..Protein.Groups":1,"Percolator.q.Value":0.00023,"Percolator.PEP":8.244e-07,"Percolator.SVMScore":3.827,"ptmRS..Binomial.Peptide.Score":498.43,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(6): 512.24; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+-H(3) O(4) P(9): 869.39; b1+-H(3) O(4) P(10): 998.43; y1+-H(3) O(4) P(14): 1665.76; b1+(3): 259.09; b1+(4): 316.11; b1+(5): 429.20","ptmRS..Best.Site.Probabilities":"T6(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; T(6): 100.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (I4)","Search.ID":"I","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"AGNFALHPEVVR","Annotated.Sequence":"[R].AGNFALHPEVVR.[E]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q03558","Master.Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","Protein.Accessions":"Q03558","Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.6295,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":437.23817,"Contaminant":"False","Aligned.m.z..Da.":437.23813,"MH...Da.":1309.69995,"Theo..MH...Da.":1309.70114,"DeltaM..ppm.":-0.91,"Deltam.z..Da.":-0.0004,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2873430.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":14.4502,"Ion.Inject.Time..ms.":22,"RT..min.":10.0451,"First.Scan":8391,"Last.Scan":8391,"Master.Scan.s.":8381,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_04.raw","File.ID":"F28","Quan.Info":"","Peptides.Matched":382,"XCorr":2.78,"X..Protein.Groups":1,"Percolator.q.Value":0.00023,"Percolator.PEP":0.00002,"Percolator.SVMScore":2.689,"Precursor.Area":1688341.125,"Apex.RT..min.":10.04,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (I4)","Search.ID":"I","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEySDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); Y12(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.2967,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40492,"Contaminant":"False","Aligned.m.z..Da.":967.40491,"MH...Da.":2900.20021,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":0.3,"Deltam.z..Da.":0.00029,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":367618.625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":73.63503,"Ion.Inject.Time..ms.":22,"RT..min.":16.1354,"First.Scan":16308,"Last.Scan":16308,"Master.Scan.s.":16298,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_04.raw","File.ID":"F28","Quan.Info":"","Peptides.Matched":2490,"XCorr":4.18,"X..Protein.Groups":1,"Percolator.q.Value":0.00023,"Percolator.PEP":8.708e-06,"Percolator.SVMScore":2.984,"ptmRS..Binomial.Peptide.Score":325.22,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"y2+-H(3) O(4) P(14): 811.37; b1+(12): 1344.56","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (I4)","Search.ID":"I","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].msEGITDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S2(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1126,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1096.47194,"Contaminant":"False","Aligned.m.z..Da.":1096.47193,"MH...Da.":2191.9366,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":3.99,"Deltam.z..Da.":0.00438,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":338950.875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":16.0545,"First.Scan":16206,"Last.Scan":16206,"Master.Scan.s.":16194,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_04.raw","File.ID":"F28","Quan.Info":"","Peptides.Matched":2169,"XCorr":3.64,"X..Protein.Groups":1,"Percolator.q.Value":0.00023,"Percolator.PEP":1.432e-06,"Percolator.SVMScore":3.63,"ptmRS..Binomial.Peptide.Score":491.59,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(2): 112.04; b1+-H(3) O(4) P(3): 241.08; b1+-H(3) O(4) P(4): 298.10; b1+-H(3) O(4) P(5): 411.19; b1+-H(3) O(4) P(6): 512.24; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+-H(3) O(4) P(9): 869.39; b1+-H(3) O(4) P(11): 1085.46; b1+(4): 396.08; b1+(5): 509.16; y1+(14): 1683.77","ptmRS..Best.Site.Probabilities":"S2(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 100.0; T(6): 0.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (I4)","Search.ID":"I","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FDDMELDENLLR","Annotated.Sequence":"[K].FDDMELDENLLR.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5252,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":755.34985,"Contaminant":"False","Aligned.m.z..Da.":755.34984,"MH...Da.":1509.69242,"Theo..MH...Da.":1509.68898,"DeltaM..ppm.":2.28,"Deltam.z..Da.":0.00172,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":631572.4375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":8.32334,"Ion.Inject.Time..ms.":22,"RT..min.":16.0501,"First.Scan":16199,"Last.Scan":16199,"Master.Scan.s.":16194,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_04.raw","File.ID":"F28","Quan.Info":"","Peptides.Matched":1094,"XCorr":3.18,"X..Protein.Groups":1,"Percolator.q.Value":0.00023,"Percolator.PEP":0.00009,"Percolator.SVMScore":2.152,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (I4)","Search.ID":"I","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0023,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40431,"Contaminant":"False","Aligned.m.z..Da.":967.4043,"MH...Da.":2900.19839,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":-0.32,"Deltam.z..Da.":-0.00031,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":364243.28125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":16.0783,"First.Scan":16234,"Last.Scan":16234,"Master.Scan.s.":16233,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_04.raw","File.ID":"F28","Quan.Info":"","Peptides.Matched":2434,"XCorr":4.41,"X..Protein.Groups":1,"Percolator.q.Value":0.00023,"Percolator.PEP":0.00001,"Percolator.SVMScore":2.864,"Precursor.Area":1154990.875,"Apex.RT..min.":15.81,"ptmRS..Binomial.Peptide.Score":308.22,"ptmRS..Isoform.Confidence.Probability":0.5,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"Y12(Phospho): 50; S13(Phospho): 50","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 50.0; S(13): 50.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (I4)","Search.ID":"I","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":5,"DeltaScore":0.087,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40397,"Contaminant":"False","Aligned.m.z..Da.":967.40399,"MH...Da.":2900.19736,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":-0.68,"Deltam.z..Da.":-0.00066,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":10086378,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":10.02882,"Ion.Inject.Time..ms.":17.334,"RT..min.":15.1603,"First.Scan":15083,"Last.Scan":15083,"Master.Scan.s.":15076,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_04.raw","File.ID":"F28","Quan.Info":"","Peptides.Matched":2405,"XCorr":7.36,"X..Protein.Groups":1,"Percolator.q.Value":0.00023,"Percolator.PEP":5.548e-10,"Percolator.SVMScore":6.438,"Precursor.Area":54447288,"Apex.RT..min.":15.1,"ptmRS..Binomial.Peptide.Score":776.7,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(13): 1413.58; b1+-H(3) O(4) P(14): 1528.61; b1+-H(3) O(4) P(15): 1642.65; b1+-H(3) O(4) P(16): 1771.69; b1+-H(3) O(4) P(18): 2028.79; y1+-H(3) O(4) P(15): 1750.78; y1+-H(3) O(4) P(14): 1621.73; y1+-H(3) O(4) P(13): 1458.67; b2+-H(3) O(4) P(16): 886.35; b2+-H(3) O(4) P(17): 950.38; b2+-H(3) O(4) P(18): 1014.90; y2+-H(3) O(4) P(14): 811.37; b1+(12): 1344.56; y1+(13): 1556.65","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (I4)","Search.ID":"I","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"AAEAGETGAATSATEGDNNNNTAAGDKK","Annotated.Sequence":"[K].AAEAGETGAATsATEGDNNNNTAAGDKK.[G]","Modifications":"S12(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0419,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":906.04949,"Contaminant":"False","Aligned.m.z..Da.":906.04948,"MH...Da.":2716.13391,"Theo..MH...Da.":2716.13298,"DeltaM..ppm.":0.34,"Deltam.z..Da.":0.00031,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":4300744,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":5.0095,"First.Scan":1743,"Last.Scan":1743,"Master.Scan.s.":1738,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_04.raw","File.ID":"F28","Quan.Info":"","Peptides.Matched":2558,"XCorr":7.16,"X..Protein.Groups":1,"Percolator.q.Value":0.00023,"Percolator.PEP":6.51e-10,"Percolator.SVMScore":6.381,"Precursor.Area":4620420,"Apex.RT..min.":5.02,"ptmRS..Binomial.Peptide.Score":519.39,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(15): 1300.57; y1+-H(3) O(4) P(18): 1789.79; y1+-H(3) O(4) P(17): 1688.75; y2+-H(3) O(4) P(25): 1174.02; y2+-H(3) O(4) P(24): 1138.50; y2+-H(3) O(4) P(22): 1045.47; y2+-H(3) O(4) P(21): 994.95; y2+-H(3) O(4) P(18): 895.40; y2+-H(3) O(4) P(17): 844.88","ptmRS..Best.Site.Probabilities":"S12(Phospho): 99.99","ptmRS..Phospho.Site.Probabilities":"T(7): 0.0; T(11): 0.0; S(12): 100.0; T(14): 0.0; T(22): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AD4)","Search.ID":"AD","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"AAEAGETGAATSATEGDNNNNTAAGDKK","Annotated.Sequence":"[K].AAEAGETGAATsATEGDNNNNTAAGDKK.[G]","Modifications":"S12(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0207,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":906.04953,"Contaminant":"False","Aligned.m.z..Da.":906.04946,"MH...Da.":2716.13403,"Theo..MH...Da.":2716.13298,"DeltaM..ppm.":0.39,"Deltam.z..Da.":0.00035,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2940458.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":5.035,"First.Scan":1780,"Last.Scan":1780,"Master.Scan.s.":1774,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_01.raw","File.ID":"F7","Quan.Info":"","Peptides.Matched":2560,"XCorr":6.77,"X..Protein.Groups":1,"Percolator.q.Value":0.00024,"Percolator.PEP":4.618e-13,"Percolator.SVMScore":6.055,"ptmRS..Binomial.Peptide.Score":472.37,"ptmRS..Isoform.Confidence.Probability":0.985,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(15): 1300.57; y2+-H(3) O(4) P(24): 1138.50; y2+-H(3) O(4) P(21): 994.95; y2+-H(3) O(4) P(18): 895.40; y2+-H(3) O(4) P(17): 844.88","ptmRS..Best.Site.Probabilities":"S12(Phospho): 98.5","ptmRS..Phospho.Site.Probabilities":"T(7): 0.8; T(11): 0.8; S(12): 98.5; T(14): 0.0; T(22): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AD4)","Search.ID":"AD","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"HSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[R].HSDSySENETNHTNVPISSTGGTNNK.[T]","Modifications":"Y5(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":0,"Charge":4,"Original.Precursor.Charge":4,"DeltaScore":0.0053,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":718.30246,"Contaminant":"False","Aligned.m.z..Da.":718.30251,"MH...Da.":2870.18802,"Theo..MH...Da.":2870.18608,"DeltaM..ppm.":0.68,"Deltam.z..Da.":0.00049,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2087816.125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":12.47157,"Ion.Inject.Time..ms.":22,"RT..min.":7.6672,"First.Scan":5433,"Last.Scan":5433,"Master.Scan.s.":5427,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_01.raw","File.ID":"F7","Quan.Info":"","Peptides.Matched":2519,"XCorr":5.64,"X..Protein.Groups":1,"Percolator.q.Value":0.00024,"Percolator.PEP":1.197e-07,"Percolator.SVMScore":3.153,"Precursor.Area":1546690.375,"Apex.RT..min.":7.67,"ptmRS..Binomial.Peptide.Score":275.11,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(6): 659.24; b1+-H(3) O(4) P(9): 1031.37; b1+-H(3) O(4) P(11): 1246.46; b2+-H(3) O(4) P(7): 394.65; b2+-H(3) O(4) P(8): 451.67; b2+-H(3) O(4) P(13): 742.79; b2+-H(3) O(4) P(14): 799.81; b2+-H(3) O(4) P(15): 849.34; b2+-H(3) O(4) P(17): 954.41; b1+(4): 427.16","ptmRS..Best.Site.Probabilities":"S6(Phospho): 99.27","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(4): 0.7; Y(5): 0.0; S(6): 99.3; T(10): 0.0; T(13): 0.0; S(18): 0.0; S(19): 0.0; T(20): 0.0; T(23): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AD4)","Search.ID":"AD","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"IQDPQMTGYVSTR","Annotated.Sequence":"[R].IQDPQmTGyVSTR.[Y]","Modifications":"M6(Oxidation); Y9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P32485","Master.Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","Protein.Accessions":"P32485","Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1635,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":796.34637,"Contaminant":"False","Aligned.m.z..Da.":796.34643,"MH...Da.":1591.68547,"Theo..MH...Da.":1591.6822,"DeltaM..ppm.":2.06,"Deltam.z..Da.":0.00164,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1231941.125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":7.7174,"First.Scan":5507,"Last.Scan":5507,"Master.Scan.s.":5505,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_01.raw","File.ID":"F7","Quan.Info":"","Peptides.Matched":1170,"XCorr":2.63,"X..Protein.Groups":1,"Percolator.q.Value":0.00024,"Percolator.PEP":0.00007,"Percolator.SVMScore":1.665,"Precursor.Area":1504729.25,"Apex.RT..min.":7.7,"ptmRS..Binomial.Peptide.Score":267.4,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+(6): 762.32; y1+(5): 705.30","ptmRS..Best.Site.Probabilities":"Y9(Phospho): 99.99","ptmRS..Phospho.Site.Probabilities":"T(7): 0.0; Y(9): 100.0; S(11): 0.0; T(12): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AD4)","Search.ID":"AD","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"AIMPIIEGHDVLAQAQSGTGK","Annotated.Sequence":"[R].AImPIIEGHDVLAQAQSGTGK.[T]","Modifications":"M3(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.7031,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":718.04134,"Contaminant":"False","Aligned.m.z..Da.":718.04134,"MH...Da.":2152.10947,"Theo..MH...Da.":2152.10668,"DeltaM..ppm.":1.3,"Deltam.z..Da.":0.00093,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":201184.09375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":10.57221,"Ion.Inject.Time..ms.":22,"RT..min.":12.7699,"First.Scan":11777,"Last.Scan":11777,"Master.Scan.s.":11770,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_01.raw","File.ID":"F7","Quan.Info":"","Peptides.Matched":1003,"XCorr":3.57,"X..Protein.Groups":1,"Percolator.q.Value":0.00024,"Percolator.PEP":2.598e-08,"Percolator.SVMScore":3.509,"Precursor.Area":236982.67188,"Apex.RT..min.":12.77,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AD4)","Search.ID":"AD","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DIFSNDELLSDAYDAK","Annotated.Sequence":"[K].DIFSNDELLSDAYDAK.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7478,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":908.41849,"Contaminant":"False","Aligned.m.z..Da.":908.41814,"MH...Da.":1815.82971,"Theo..MH...Da.":1815.82831,"DeltaM..ppm.":0.77,"Deltam.z..Da.":0.0007,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":305103.53125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":19.88335,"Ion.Inject.Time..ms.":22,"RT..min.":17.0541,"First.Scan":15760,"Last.Scan":15760,"Master.Scan.s.":15757,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_01.raw","File.ID":"F7","Quan.Info":"","Peptides.Matched":1570,"XCorr":3.41,"X..Protein.Groups":1,"Percolator.q.Value":0.00024,"Percolator.PEP":2.031e-07,"Percolator.SVMScore":3.03,"Precursor.Area":692044.625,"Apex.RT..min.":17.08,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AD4)","Search.ID":"AD","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DIFSNDELLSDAYDAK","Annotated.Sequence":"[K].DIFSNDELLSDAYDAK.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.6776,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":605.94816,"Contaminant":"False","Aligned.m.z..Da.":605.94793,"MH...Da.":1815.82995,"Theo..MH...Da.":1815.82831,"DeltaM..ppm.":0.9,"Deltam.z..Da.":0.00054,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":245094.84375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":7.47628,"Ion.Inject.Time..ms.":22,"RT..min.":17.0716,"First.Scan":15777,"Last.Scan":15777,"Master.Scan.s.":15773,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_01.raw","File.ID":"F7","Quan.Info":"","Peptides.Matched":1620,"XCorr":2.14,"X..Protein.Groups":1,"Percolator.q.Value":0.00024,"Percolator.PEP":0.00023,"Percolator.SVMScore":1.378,"Precursor.Area":153228.4375,"Apex.RT..min.":17.08,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AD4)","Search.ID":"AD","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ILISTDLLAR","Annotated.Sequence":"[R].ILISTDLLAR.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5636,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":557.84553,"Contaminant":"False","Aligned.m.z..Da.":557.84541,"MH...Da.":1114.68379,"Theo..MH...Da.":1114.68304,"DeltaM..ppm.":0.67,"Deltam.z..Da.":0.00038,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":347142.0625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":37.03157,"Ion.Inject.Time..ms.":22,"RT..min.":15.6099,"First.Scan":14407,"Last.Scan":14407,"Master.Scan.s.":14405,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_01.raw","File.ID":"F7","Quan.Info":"","Peptides.Matched":124,"XCorr":2.2,"X..Protein.Groups":1,"Percolator.q.Value":0.00024,"Percolator.PEP":0.00013,"Percolator.SVMScore":1.526,"Precursor.Area":256008.40625,"Apex.RT..min.":15.6,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AD4)","Search.ID":"AD","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"GVFGYGFEEPSAIQQR","Annotated.Sequence":"[R].GVFGYGFEEPSAIQQR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7422,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":892.93435,"Contaminant":"False","Aligned.m.z..Da.":892.93422,"MH...Da.":1784.86142,"Theo..MH...Da.":1784.86022,"DeltaM..ppm.":0.67,"Deltam.z..Da.":0.0006,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":250339.3125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":15.0099,"First.Scan":13817,"Last.Scan":13817,"Master.Scan.s.":13808,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_01.raw","File.ID":"F7","Quan.Info":"","Peptides.Matched":1410,"XCorr":3.22,"X..Protein.Groups":1,"Percolator.q.Value":0.00024,"Percolator.PEP":3.553e-07,"Percolator.SVMScore":2.9,"Precursor.Area":298630.1875,"Apex.RT..min.":15.02,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AD4)","Search.ID":"AD","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.3982,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40464,"Contaminant":"False","Aligned.m.z..Da.":967.4045,"MH...Da.":2900.19937,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":0.01,"Deltam.z..Da.":1e-05,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":142755.90625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":26.06104,"Ion.Inject.Time..ms.":22,"RT..min.":15.0158,"First.Scan":13823,"Last.Scan":13823,"Master.Scan.s.":13821,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_01.raw","File.ID":"F7","Quan.Info":"","Peptides.Matched":2489,"XCorr":3.39,"X..Protein.Groups":1,"Percolator.q.Value":0.00024,"Percolator.PEP":0.00004,"Percolator.SVMScore":1.823,"Precursor.Area":1520574.125,"Apex.RT..min.":15.09,"ptmRS..Binomial.Peptide.Score":331.38,"ptmRS..Isoform.Confidence.Probability":0.993,"ptmRS..Isoform.Group.Report":"y2+-H(3) O(4) P(14): 811.37","ptmRS..Best.Site.Probabilities":"S13(Phospho): 99.3","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.7; S(13): 99.3; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AD4)","Search.ID":"AD","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FTVSAIYSDLPQQER","Annotated.Sequence":"[K].FTVSAIYSDLPQQER.[D]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":877.44016,"Contaminant":"False","Aligned.m.z..Da.":877.44004,"MH...Da.":1753.87305,"Theo..MH...Da.":1753.87554,"DeltaM..ppm.":-1.42,"Deltam.z..Da.":-0.00125,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":379613.78125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":18.34935,"Ion.Inject.Time..ms.":22,"RT..min.":15.0165,"First.Scan":13824,"Last.Scan":13824,"Master.Scan.s.":13821,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_01.raw","File.ID":"F7","Quan.Info":"","Peptides.Matched":1146,"XCorr":3.1,"X..Protein.Groups":1,"Percolator.q.Value":0.00024,"Percolator.PEP":0.00002,"Percolator.SVMScore":1.926,"Precursor.Area":191550.98438,"Apex.RT..min.":15.02,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AD4)","Search.ID":"AD","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MFILDEADEMLSSGFK","Annotated.Sequence":"[K].mFILDEADEmLSSGFK.[E]","Modifications":"M1(Oxidation); M10(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.4798,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":932.92298,"Contaminant":"False","Aligned.m.z..Da.":932.92279,"MH...Da.":1864.83868,"Theo..MH...Da.":1864.83433,"DeltaM..ppm.":2.33,"Deltam.z..Da.":0.00218,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":148965.0625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":12.45903,"Ion.Inject.Time..ms.":22,"RT..min.":16.6823,"First.Scan":15430,"Last.Scan":15430,"Master.Scan.s.":15427,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_01.raw","File.ID":"F7","Quan.Info":"","Peptides.Matched":1796,"XCorr":1.98,"X..Protein.Groups":1,"Percolator.q.Value":0.00024,"Percolator.PEP":0.00013,"Percolator.SVMScore":1.515,"Precursor.Area":138539.90625,"Apex.RT..min.":16.67,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AD4)","Search.ID":"AD","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].msEGITDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S2(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1604,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1096.46445,"Contaminant":"False","Aligned.m.z..Da.":1096.46425,"MH...Da.":2191.92164,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":-2.83,"Deltam.z..Da.":-0.0031,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":224552.28125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":15.7387,"First.Scan":14529,"Last.Scan":14529,"Master.Scan.s.":14528,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_01.raw","File.ID":"F7","Quan.Info":"","Peptides.Matched":1878,"XCorr":3.99,"X..Protein.Groups":1,"Percolator.q.Value":0.00024,"Percolator.PEP":1.809e-06,"Percolator.SVMScore":2.521,"Precursor.Area":2129410.5,"Apex.RT..min.":15.8,"ptmRS..Binomial.Peptide.Score":463.79,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(2): 112.04; b1+-H(3) O(4) P(3): 241.08; b1+-H(3) O(4) P(4): 298.10; b1+-H(3) O(4) P(5): 411.19; b1+-H(3) O(4) P(6): 512.24; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+-H(3) O(4) P(9): 869.39; b1+-H(3) O(4) P(10): 998.43; y1+(14): 1683.77","ptmRS..Best.Site.Probabilities":"S2(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 100.0; T(6): 0.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AD4)","Search.ID":"AD","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FDDMELDENLLR","Annotated.Sequence":"[K].FDDMELDENLLR.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5764,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":755.3465,"Contaminant":"False","Aligned.m.z..Da.":755.34645,"MH...Da.":1509.68572,"Theo..MH...Da.":1509.68898,"DeltaM..ppm.":-2.16,"Deltam.z..Da.":-0.00163,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":250811.76562,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":35.06099,"Ion.Inject.Time..ms.":22,"RT..min.":16.0582,"First.Scan":14854,"Last.Scan":14854,"Master.Scan.s.":14845,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_01.raw","File.ID":"F7","Quan.Info":"","Peptides.Matched":1023,"XCorr":2.29,"X..Protein.Groups":1,"Percolator.q.Value":0.00024,"Percolator.PEP":0.00009,"Percolator.SVMScore":1.612,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AD4)","Search.ID":"AD","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEySDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); Y12(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.4458,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40932,"Contaminant":"False","Aligned.m.z..Da.":967.40927,"MH...Da.":2900.21342,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":4.86,"Deltam.z..Da.":0.0047,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":105001.40625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":10.56929,"Ion.Inject.Time..ms.":22,"RT..min.":16.085,"First.Scan":14886,"Last.Scan":14886,"Master.Scan.s.":14884,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_01.raw","File.ID":"F7","Quan.Info":"","Peptides.Matched":2756,"XCorr":3.23,"X..Protein.Groups":1,"Percolator.q.Value":0.00024,"Percolator.PEP":0.00003,"Percolator.SVMScore":1.904,"Precursor.Area":143246.60938,"Apex.RT..min.":16.05,"ptmRS..Binomial.Peptide.Score":267.7,"ptmRS..Isoform.Confidence.Probability":0.008,"ptmRS..Isoform.Group.Report":"y2+-H(3) O(4) P(14): 811.37","ptmRS..Best.Site.Probabilities":"S13(Phospho): 99.2","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.8; S(13): 99.2; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AD4)","Search.ID":"AD","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"APQALMLAPTR","Annotated.Sequence":"[K].APQALmLAPTR.[E]","Modifications":"M6(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6802,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":592.82706,"Contaminant":"False","Aligned.m.z..Da.":592.82702,"MH...Da.":1184.64685,"Theo..MH...Da.":1184.6456,"DeltaM..ppm.":1.05,"Deltam.z..Da.":0.00062,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":642511.375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":11.47999,"Ion.Inject.Time..ms.":22,"RT..min.":10.1968,"First.Scan":8457,"Last.Scan":8457,"Master.Scan.s.":8456,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_01.raw","File.ID":"F7","Quan.Info":"","Peptides.Matched":375,"XCorr":2.47,"X..Protein.Groups":1,"Percolator.q.Value":0.00024,"Percolator.PEP":0.00023,"Percolator.SVMScore":1.379,"Precursor.Area":565434.4375,"Apex.RT..min.":10.2,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AD4)","Search.ID":"AD","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KDELTLEGIK","Annotated.Sequence":"[K].KDELTLEGIK.[Q]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.4924,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":573.32476,"Contaminant":"False","Aligned.m.z..Da.":573.32472,"MH...Da.":1145.64225,"Theo..MH...Da.":1145.64123,"DeltaM..ppm.":0.89,"Deltam.z..Da.":0.00051,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1091412.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":2.82467,"Ion.Inject.Time..ms.":22,"RT..min.":10.2538,"First.Scan":8527,"Last.Scan":8527,"Master.Scan.s.":8521,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_01.raw","File.ID":"F7","Quan.Info":"","Peptides.Matched":301,"XCorr":3.29,"X..Protein.Groups":1,"Percolator.q.Value":0.00024,"Percolator.PEP":1.039e-06,"Percolator.SVMScore":2.65,"Precursor.Area":643273.1875,"Apex.RT..min.":10.25,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AD4)","Search.ID":"AD","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"IQDPQMTGYVSTR","Annotated.Sequence":"[R].IQDPQMTGyVSTR.[Y]","Modifications":"Y9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P32485","Master.Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","Protein.Accessions":"P32485","Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1349,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":788.34511,"Contaminant":"False","Aligned.m.z..Da.":788.34509,"MH...Da.":1575.68295,"Theo..MH...Da.":1575.68728,"DeltaM..ppm.":-2.75,"Deltam.z..Da.":-0.00217,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1025977.1875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":9.5694,"First.Scan":7714,"Last.Scan":7714,"Master.Scan.s.":7702,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_01.raw","File.ID":"F7","Quan.Info":"","Peptides.Matched":1147,"XCorr":2.52,"X..Protein.Groups":1,"Percolator.q.Value":0.00024,"Percolator.PEP":0.00004,"Percolator.SVMScore":1.782,"Precursor.Area":1102646.75,"Apex.RT..min.":9.57,"ptmRS..Binomial.Peptide.Score":287.77,"ptmRS..Isoform.Confidence.Probability":0.991,"ptmRS..Isoform.Group.Report":"y1+(4): 462.27; y1+(3): 363.20","ptmRS..Best.Site.Probabilities":"Y9(Phospho): 99.08","ptmRS..Phospho.Site.Probabilities":"T(7): 0.0; Y(9): 99.1; S(11): 0.5; T(12): 0.5"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (G4)","Search.ID":"G","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LEAGLSDSK","Annotated.Sequence":"[K].LEAGLsDSK.[Q]","Modifications":"S6(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.0815,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":500.22345,"Contaminant":"False","Aligned.m.z..Da.":500.2235,"MH...Da.":999.43963,"Theo..MH...Da.":999.43943,"DeltaM..ppm.":0.2,"Deltam.z..Da.":0.0001,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":4457859.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":12.23582,"Ion.Inject.Time..ms.":22,"RT..min.":7.6849,"First.Scan":5861,"Last.Scan":5861,"Master.Scan.s.":5857,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_06.raw","File.ID":"F30","Quan.Info":"","Peptides.Matched":652,"XCorr":2.7,"X..Protein.Groups":1,"Percolator.q.Value":0.00026,"Percolator.PEP":0.00071,"Percolator.SVMScore":1.324,"Precursor.Area":3264099.75,"Apex.RT..min.":7.69,"ptmRS..Binomial.Peptide.Score":259.25,"ptmRS..Isoform.Confidence.Probability":0.996,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(8): 788.38; y1+-H(3) O(4) P(7): 659.34; y1+-H(3) O(4) P(6): 588.30; y1+-H(3) O(4) P(5): 531.28; y1+-H(3) O(4) P(4): 418.19; y2+-H(3) O(4) P(6): 294.65; y1+(3): 349.17; y1+(2): 234.14","ptmRS..Best.Site.Probabilities":"S6(Phospho): 99.62","ptmRS..Phospho.Site.Probabilities":"S(6): 99.6; S(8): 0.4"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (G4)","Search.ID":"G","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"NQQLEDEIEK","Annotated.Sequence":"[K].NQQLEDEIEK.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.3636,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":623.30115,"Contaminant":"False","Aligned.m.z..Da.":623.30126,"MH...Da.":1245.59504,"Theo..MH...Da.":1245.59574,"DeltaM..ppm.":-0.56,"Deltam.z..Da.":-0.00035,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3566363,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":60.65531,"Ion.Inject.Time..ms.":19.57,"RT..min.":8.5997,"First.Scan":7035,"Last.Scan":7035,"Master.Scan.s.":7027,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_06.raw","File.ID":"F30","Quan.Info":"","Peptides.Matched":641,"XCorr":1.65,"X..Protein.Groups":1,"Percolator.q.Value":0.00026,"Percolator.PEP":0.00087,"Percolator.SVMScore":1.27,"Precursor.Area":2124567,"Apex.RT..min.":8.59,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (G4)","Search.ID":"G","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DFLLKPELSR","Annotated.Sequence":"[K].DFLLKPELSR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5954,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":609.34759,"Contaminant":"False","Aligned.m.z..Da.":609.34752,"MH...Da.":1217.68791,"Theo..MH...Da.":1217.68885,"DeltaM..ppm.":-0.77,"Deltam.z..Da.":-0.00047,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":838118,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":38.44209,"Ion.Inject.Time..ms.":22,"RT..min.":13.1743,"First.Scan":13165,"Last.Scan":13165,"Master.Scan.s.":13163,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_06.raw","File.ID":"F30","Quan.Info":"","Peptides.Matched":285,"XCorr":1.73,"X..Protein.Groups":1,"Percolator.q.Value":0.00026,"Percolator.PEP":0.00057,"Percolator.SVMScore":1.379,"Precursor.Area":684213.5,"Apex.RT..min.":13.17,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (G4)","Search.ID":"G","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"NFASLEHSR","Annotated.Sequence":"[K].NFASLEHsR.[S]","Modifications":"S8(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.365,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":570.74581,"Contaminant":"False","Aligned.m.z..Da.":570.7459,"MH...Da.":1140.48434,"Theo..MH...Da.":1140.48336,"DeltaM..ppm.":0.86,"Deltam.z..Da.":0.00049,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3512017.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":2.43893,"Ion.Inject.Time..ms.":22,"RT..min.":8.356,"First.Scan":6721,"Last.Scan":6721,"Master.Scan.s.":6715,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_06.raw","File.ID":"F30","Quan.Info":"","Peptides.Matched":460,"XCorr":2.74,"X..Protein.Groups":1,"Percolator.q.Value":0.00026,"Percolator.PEP":0.00122,"Percolator.SVMScore":1.18,"ptmRS..Binomial.Peptide.Score":337.88,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(8): 928.46; y1+-H(3) O(4) P(7): 781.40; y1+-H(3) O(4) P(6): 710.36; y1+-H(3) O(4) P(5): 623.33; y1+-H(3) O(4) P(4): 510.24; y1+-H(3) O(4) P(3): 381.20; y1+-H(3) O(4) P(2): 244.14; y2+-H(3) O(4) P(7): 391.20; y2+-H(3) O(4) P(6): 355.68; y2+-H(3) O(4) P(5): 312.17; y2+-H(3) O(4) P(3): 191.10; y1+(5): 721.30","ptmRS..Best.Site.Probabilities":"S8(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(4): 0.0; S(8): 100.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (G4)","Search.ID":"G","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LEAGLSDSK","Annotated.Sequence":"[K].LEAGLSDSK.[Q]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.3098,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":460.23981,"Contaminant":"False","Aligned.m.z..Da.":460.23979,"MH...Da.":919.47235,"Theo..MH...Da.":919.4731,"DeltaM..ppm.":-0.82,"Deltam.z..Da.":-0.00038,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1777382.375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":33.75974,"Ion.Inject.Time..ms.":22,"RT..min.":6.595,"First.Scan":4304,"Last.Scan":4304,"Master.Scan.s.":4297,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_06.raw","File.ID":"F30","Quan.Info":"","Peptides.Matched":400,"XCorr":1.84,"X..Protein.Groups":1,"Percolator.q.Value":0.00026,"Percolator.PEP":0.00142,"Percolator.SVMScore":1.14,"Precursor.Area":1527902.625,"Apex.RT..min.":6.6,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (T4)","Search.ID":"T","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"AAEAGETGAATSATEGDNNNNTAAGDKK","Annotated.Sequence":"[K].AAEAGETGAATSATEGDNNNNTAAGDKK.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.7353,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":879.39385,"Contaminant":"False","Aligned.m.z..Da.":879.39384,"MH...Da.":2636.167,"Theo..MH...Da.":2636.16665,"DeltaM..ppm.":0.13,"Deltam.z..Da.":0.00012,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1972195.125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":4.9757,"First.Scan":1991,"Last.Scan":1991,"Master.Scan.s.":1990,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_05.raw","File.ID":"F17","Quan.Info":"","Peptides.Matched":3566,"XCorr":7.63,"X..Protein.Groups":1,"Percolator.q.Value":0.00027,"Percolator.PEP":1.50358e-21,"Percolator.SVMScore":9.15919,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (T4)","Search.ID":"T","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"AIMPIIEGHDVLAQAQSGTGK","Annotated.Sequence":"[R].AImPIIEGHDVLAQAQSGTGK.[T]","Modifications":"M3(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.7383,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":718.04257,"Contaminant":"False","Aligned.m.z..Da.":718.04231,"MH...Da.":2152.11319,"Theo..MH...Da.":2152.10668,"DeltaM..ppm.":3.02,"Deltam.z..Da.":0.00217,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":317550.78125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":4.77866,"Ion.Inject.Time..ms.":22,"RT..min.":12.65,"First.Scan":11958,"Last.Scan":11958,"Master.Scan.s.":11947,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_05.raw","File.ID":"F17","Quan.Info":"","Peptides.Matched":951,"XCorr":4.7,"X..Protein.Groups":1,"Percolator.q.Value":0.00027,"Percolator.PEP":6.566e-13,"Percolator.SVMScore":5.205,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (T4)","Search.ID":"T","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"AAEAGETGAATSATEGDNNNNTAAGDKK","Annotated.Sequence":"[K].AAEAGETGAATsATEGDNNNNTAAGDKK.[G]","Modifications":"S12(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0452,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":906.04906,"Contaminant":"False","Aligned.m.z..Da.":906.04908,"MH...Da.":2716.13263,"Theo..MH...Da.":2716.13298,"DeltaM..ppm.":-0.13,"Deltam.z..Da.":-0.00012,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2347020.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":3.14193,"Ion.Inject.Time..ms.":22,"RT..min.":5.0458,"First.Scan":2084,"Last.Scan":2084,"Master.Scan.s.":2081,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_05.raw","File.ID":"F17","Quan.Info":"","Peptides.Matched":2553,"XCorr":6.64,"X..Protein.Groups":1,"Percolator.q.Value":0.00027,"Percolator.PEP":1.434e-16,"Percolator.SVMScore":6.88,"ptmRS..Binomial.Peptide.Score":544.16,"ptmRS..Isoform.Confidence.Probability":0.993,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(15): 1300.57; y1+-H(3) O(4) P(18): 1789.79; y1+-H(3) O(4) P(17): 1688.75; y2+-H(3) O(4) P(25): 1174.02; y2+-H(3) O(4) P(24): 1138.50; y2+-H(3) O(4) P(22): 1045.47; y2+-H(3) O(4) P(18): 895.40; y2+-H(3) O(4) P(17): 844.88","ptmRS..Best.Site.Probabilities":"S12(Phospho): 99.28","ptmRS..Phospho.Site.Probabilities":"T(7): 0.7; T(11): 0.0; S(12): 99.3; T(14): 0.0; T(22): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (T4)","Search.ID":"T","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LQETNPEEVPKFEK","Annotated.Sequence":"[K].LQETNPEEVPKFEK.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.6089,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":563.29018,"Contaminant":"False","Aligned.m.z..Da.":563.29021,"MH...Da.":1687.85599,"Theo..MH...Da.":1687.85374,"DeltaM..ppm.":1.33,"Deltam.z..Da.":0.00075,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1032871.5625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":73.41158,"Ion.Inject.Time..ms.":22,"RT..min.":9.323,"First.Scan":8119,"Last.Scan":8119,"Master.Scan.s.":8113,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_05.raw","File.ID":"F17","Quan.Info":"","Peptides.Matched":836,"XCorr":2.25,"X..Protein.Groups":1,"Percolator.q.Value":0.00027,"Percolator.PEP":0.00014,"Percolator.SVMScore":1.394,"Precursor.Area":645557.5625,"Apex.RT..min.":9.32,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (T4)","Search.ID":"T","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"IQDPQMTGYVSTR","Annotated.Sequence":"[R].IQDPQMTGyVSTR.[Y]","Modifications":"Y9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P32485","Master.Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","Protein.Accessions":"P32485","Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1613,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":788.34503,"Contaminant":"False","Aligned.m.z..Da.":788.34508,"MH...Da.":1575.68279,"Theo..MH...Da.":1575.68728,"DeltaM..ppm.":-2.85,"Deltam.z..Da.":-0.00225,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":597498.5625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":33.41161,"Ion.Inject.Time..ms.":22,"RT..min.":9.483,"First.Scan":8344,"Last.Scan":8344,"Master.Scan.s.":8334,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_05.raw","File.ID":"F17","Quan.Info":"","Peptides.Matched":1151,"XCorr":2.48,"X..Protein.Groups":1,"Percolator.q.Value":0.00027,"Percolator.PEP":0.00006,"Percolator.SVMScore":1.546,"Precursor.Area":881881.875,"Apex.RT..min.":9.5,"ptmRS..Binomial.Peptide.Score":259.96,"ptmRS..Isoform.Confidence.Probability":0.497,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(10): 1121.54; b1+(7): 894.34","ptmRS..Best.Site.Probabilities":"T7(Phospho): 49.73; Y9(Phospho): 49.73","ptmRS..Phospho.Site.Probabilities":"T(7): 49.7; Y(9): 49.7; S(11): 0.3; T(12): 0.3"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (T4)","Search.ID":"T","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"HSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[R].HSDSySENETNHTNVPISSTGGTNNK.[T]","Modifications":"Y5(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":0,"Charge":4,"Original.Precursor.Charge":2,"DeltaScore":0.008,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":718.30252,"Contaminant":"False","Aligned.m.z..Da.":718.30261,"MH...Da.":2870.18823,"Theo..MH...Da.":2870.18608,"DeltaM..ppm.":0.75,"Deltam.z..Da.":0.00054,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1777976.125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":29.73102,"Ion.Inject.Time..ms.":22,"RT..min.":7.5084,"First.Scan":5505,"Last.Scan":5505,"Master.Scan.s.":5500,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_05.raw","File.ID":"F17","Quan.Info":"","Peptides.Matched":2529,"XCorr":3.75,"X..Protein.Groups":1,"Percolator.q.Value":0.00027,"Percolator.PEP":3.18e-06,"Percolator.SVMScore":2.146,"Precursor.Area":2044317.125,"Apex.RT..min.":7.5,"ptmRS..Binomial.Peptide.Score":186.56,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(9): 1031.37; b1+-H(3) O(4) P(11): 1246.46; b2+-H(3) O(4) P(14): 799.81; b2+-H(3) O(4) P(15): 849.34; b1+(4): 427.16","ptmRS..Best.Site.Probabilities":"S6(Phospho): 97.72","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(4): 1.1; Y(5): 0.0; S(6): 97.7; T(10): 1.1; T(13): 0.0; S(18): 0.0; S(19): 0.0; T(20): 0.0; T(23): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (T4)","Search.ID":"T","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"TAVFYGGTPISK","Annotated.Sequence":"[K].TAVFYGGTPISK.[D]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":4,"DeltaScore":0.4576,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":620.83191,"Contaminant":"False","Aligned.m.z..Da.":620.83195,"MH...Da.":1240.65655,"Theo..MH...Da.":1240.65721,"DeltaM..ppm.":-0.53,"Deltam.z..Da.":-0.00033,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":224345.8125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":76.57969,"Ion.Inject.Time..ms.":22,"RT..min.":11.1991,"First.Scan":10532,"Last.Scan":10532,"Master.Scan.s.":10522,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_05.raw","File.ID":"F17","Quan.Info":"","Peptides.Matched":414,"XCorr":2.36,"X..Protein.Groups":1,"Percolator.q.Value":0.00027,"Percolator.PEP":0.00036,"Percolator.SVMScore":1.203,"Precursor.Area":181909.57812,"Apex.RT..min.":11.2,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (T4)","Search.ID":"T","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KFQDDTLNR","Annotated.Sequence":"[K].KFQDDtLNR.[V]","Modifications":"T6(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6391,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":608.77213,"Contaminant":"False","Aligned.m.z..Da.":608.77221,"MH...Da.":1216.53699,"Theo..MH...Da.":1216.53579,"DeltaM..ppm.":0.98,"Deltam.z..Da.":0.0006,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":988899.375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":23.88488,"Ion.Inject.Time..ms.":22,"RT..min.":6.2221,"First.Scan":3844,"Last.Scan":3844,"Master.Scan.s.":3836,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_05.raw","File.ID":"F17","Quan.Info":"","Peptides.Matched":614,"XCorr":2.66,"X..Protein.Groups":1,"Percolator.q.Value":0.00027,"Percolator.PEP":0.00023,"Percolator.SVMScore":1.299,"Precursor.Area":1075115.375,"Apex.RT..min.":6.23,"ptmRS..Binomial.Peptide.Score":327.24,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"T6(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"T(6): 100.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (T4)","Search.ID":"T","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"APQALMLAPTR","Annotated.Sequence":"[K].APQALmLAPTR.[E]","Modifications":"M6(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.722,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":592.82671,"Contaminant":"False","Aligned.m.z..Da.":592.82675,"MH...Da.":1184.64615,"Theo..MH...Da.":1184.6456,"DeltaM..ppm.":0.46,"Deltam.z..Da.":0.00027,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":314500.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":29.14813,"Ion.Inject.Time..ms.":22,"RT..min.":10.2074,"First.Scan":9359,"Last.Scan":9359,"Master.Scan.s.":9351,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_05.raw","File.ID":"F17","Quan.Info":"","Peptides.Matched":304,"XCorr":2.59,"X..Protein.Groups":1,"Percolator.q.Value":0.00027,"Percolator.PEP":0.00006,"Percolator.SVMScore":1.554,"Precursor.Area":266898.25,"Apex.RT..min.":10.22,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (T4)","Search.ID":"T","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FDDMELDENLLR","Annotated.Sequence":"[K].FDDmELDENLLR.[G]","Modifications":"M4(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5678,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":763.34485,"Contaminant":"False","Aligned.m.z..Da.":763.34499,"MH...Da.":1525.68242,"Theo..MH...Da.":1525.6839,"DeltaM..ppm.":-0.97,"Deltam.z..Da.":-0.00074,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":443832.21875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":14.5025,"First.Scan":13214,"Last.Scan":13214,"Master.Scan.s.":13211,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_05.raw","File.ID":"F17","Quan.Info":"","Peptides.Matched":1169,"XCorr":2.73,"X..Protein.Groups":1,"Percolator.q.Value":0.00027,"Percolator.PEP":5.954e-06,"Percolator.SVMScore":2.021,"Precursor.Area":179932.875,"Apex.RT..min.":14.5,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (T4)","Search.ID":"T","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"GVFGYGFEEPSAIQQR","Annotated.Sequence":"[R].GVFGYGFEEPSAIQQR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.721,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":892.93328,"Contaminant":"False","Aligned.m.z..Da.":892.93327,"MH...Da.":1784.85929,"Theo..MH...Da.":1784.86022,"DeltaM..ppm.":-0.52,"Deltam.z..Da.":-0.00047,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":386827.8125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":15.0033,"First.Scan":13596,"Last.Scan":13596,"Master.Scan.s.":13595,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_05.raw","File.ID":"F17","Quan.Info":"","Peptides.Matched":1278,"XCorr":3.62,"X..Protein.Groups":1,"Percolator.q.Value":0.00027,"Percolator.PEP":1.066e-09,"Percolator.SVMScore":3.736,"Precursor.Area":160668.14062,"Apex.RT..min.":15.01,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (T4)","Search.ID":"T","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KDELTLEGIK","Annotated.Sequence":"[K].KDELTLEGIK.[Q]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.6406,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":382.55181,"Contaminant":"False","Aligned.m.z..Da.":382.55184,"MH...Da.":1145.64088,"Theo..MH...Da.":1145.64123,"DeltaM..ppm.":-0.3,"Deltam.z..Da.":-0.00012,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":263031.8125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":10.3162,"First.Scan":9470,"Last.Scan":9470,"Master.Scan.s.":9462,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_05.raw","File.ID":"F17","Quan.Info":"","Peptides.Matched":311,"XCorr":2.81,"X..Protein.Groups":1,"Percolator.q.Value":0.00027,"Percolator.PEP":0.00038,"Percolator.SVMScore":1.193,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (T4)","Search.ID":"T","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KDELTLEGIK","Annotated.Sequence":"[K].KDELTLEGIK.[Q]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5053,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":573.32474,"Contaminant":"False","Aligned.m.z..Da.":573.32478,"MH...Da.":1145.64221,"Theo..MH...Da.":1145.64123,"DeltaM..ppm.":0.86,"Deltam.z..Da.":0.00049,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":218198.42188,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":15.84249,"Ion.Inject.Time..ms.":22,"RT..min.":10.3075,"First.Scan":9458,"Last.Scan":9458,"Master.Scan.s.":9449,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_05.raw","File.ID":"F17","Quan.Info":"","Peptides.Matched":301,"XCorr":3.8,"X..Protein.Groups":1,"Percolator.q.Value":0.00027,"Percolator.PEP":2.944e-09,"Percolator.SVMScore":3.534,"Precursor.Area":226960.60938,"Apex.RT..min.":10.31,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (T4)","Search.ID":"T","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"AGNFALHPEVVR","Annotated.Sequence":"[R].AGNFALHPEVVR.[E]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q03558","Master.Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","Protein.Accessions":"Q03558","Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.6577,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":437.23762,"Contaminant":"False","Aligned.m.z..Da.":437.23765,"MH...Da.":1309.69831,"Theo..MH...Da.":1309.70114,"DeltaM..ppm.":-2.16,"Deltam.z..Da.":-0.00094,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":657848.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":13.48313,"Ion.Inject.Time..ms.":22,"RT..min.":10.0785,"First.Scan":9177,"Last.Scan":9177,"Master.Scan.s.":9169,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_05.raw","File.ID":"F17","Quan.Info":"","Peptides.Matched":456,"XCorr":2.6,"X..Protein.Groups":1,"Percolator.q.Value":0.00027,"Percolator.PEP":1.29e-06,"Percolator.SVMScore":2.325,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (T4)","Search.ID":"T","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FTVSAIYSDLPQQER","Annotated.Sequence":"[K].FTVSAIYSDLPQQER.[D]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6667,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":877.4406,"Contaminant":"False","Aligned.m.z..Da.":877.44062,"MH...Da.":1753.87394,"Theo..MH...Da.":1753.87554,"DeltaM..ppm.":-0.91,"Deltam.z..Da.":-0.0008,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":271436.6875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":15.32937,"Ion.Inject.Time..ms.":22,"RT..min.":14.938,"First.Scan":13552,"Last.Scan":13552,"Master.Scan.s.":13550,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_05.raw","File.ID":"F17","Quan.Info":"","Peptides.Matched":1155,"XCorr":3.18,"X..Protein.Groups":1,"Percolator.q.Value":0.00027,"Percolator.PEP":1.058e-07,"Percolator.SVMScore":2.822,"Precursor.Area":81574.1875,"Apex.RT..min.":14.92,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (T4)","Search.ID":"T","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSSPLHRLSPTGK","Annotated.Sequence":"[R].mSsPLHRLsPTGK.[S]","Modifications":"M1(Oxidation); S3(Phospho); S9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.4806,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":529.56443,"Contaminant":"False","Aligned.m.z..Da.":529.56449,"MH...Da.":1586.67874,"Theo..MH...Da.":1586.67977,"DeltaM..ppm.":-0.65,"Deltam.z..Da.":-0.00034,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1690140.875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":12.94447,"Ion.Inject.Time..ms.":22,"RT..min.":7.7055,"First.Scan":5777,"Last.Scan":5777,"Master.Scan.s.":5773,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_05.raw","File.ID":"F17","Quan.Info":"","Peptides.Matched":979,"XCorr":3.35,"X..Protein.Groups":1,"Percolator.q.Value":0.00027,"Percolator.PEP":0.00042,"Percolator.SVMScore":1.173,"Precursor.Area":2181355.25,"Apex.RT..min.":7.73,"ptmRS..Binomial.Peptide.Score":237.97,"ptmRS..Isoform.Confidence.Probability":0.992,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(8): 877.50; y1+-H(3) O(4) P(7): 740.44; y1+-H(3) O(4) P(6): 584.34; y2+-H(3) O(4) P(11): 627.82; y2+-H(3) O(4) P(10): 544.32; y2+-H(3) O(4) P(8): 439.25; y1+(4): 402.23","ptmRS..Best.Site.Probabilities":"S3(Phospho): 100; S9(Phospho): 99.2","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(3): 100.0; S(9): 99.2; T(11): 0.8"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (T4)","Search.ID":"T","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"IQDPQMTGYVSTR","Annotated.Sequence":"[R].IQDPQmTGyVSTR.[Y]","Modifications":"M6(Oxidation); Y9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P32485","Master.Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","Protein.Accessions":"P32485","Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1502,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":796.34417,"Contaminant":"False","Aligned.m.z..Da.":796.34427,"MH...Da.":1591.68107,"Theo..MH...Da.":1591.6822,"DeltaM..ppm.":-0.71,"Deltam.z..Da.":-0.00057,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1734886.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":3.786,"Ion.Inject.Time..ms.":22,"RT..min.":7.5835,"First.Scan":5606,"Last.Scan":5606,"Master.Scan.s.":5604,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_05.raw","File.ID":"F17","Quan.Info":"","Peptides.Matched":1190,"XCorr":2.73,"X..Protein.Groups":1,"Percolator.q.Value":0.00027,"Percolator.PEP":0.00005,"Percolator.SVMScore":1.611,"Precursor.Area":2131723.25,"Apex.RT..min.":7.61,"ptmRS..Binomial.Peptide.Score":275.48,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+(6): 762.32; y1+(5): 705.30","ptmRS..Best.Site.Probabilities":"Y9(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"T(7): 0.0; Y(9): 100.0; S(11): 0.0; T(12): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (T4)","Search.ID":"T","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DIFSNDELLSDAYDAK","Annotated.Sequence":"[K].DIFSNDELLSDAYDAK.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7214,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":908.41491,"Contaminant":"False","Aligned.m.z..Da.":908.41462,"MH...Da.":1815.82255,"Theo..MH...Da.":1815.82831,"DeltaM..ppm.":-3.17,"Deltam.z..Da.":-0.00288,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":390564.53125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":17.0803,"First.Scan":14814,"Last.Scan":14814,"Master.Scan.s.":14811,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_05.raw","File.ID":"F17","Quan.Info":"","Peptides.Matched":1576,"XCorr":3.59,"X..Protein.Groups":1,"Percolator.q.Value":0.00027,"Percolator.PEP":1.897e-09,"Percolator.SVMScore":3.621,"Precursor.Area":915880.0625,"Apex.RT..min.":17.11,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (T4)","Search.ID":"T","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LQETNPEEVPK","Annotated.Sequence":"[K].LQETNPEEVPK.[F]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":3,"DeltaScore":0.4977,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":642.32788,"Contaminant":"False","Aligned.m.z..Da.":642.32796,"MH...Da.":1283.64848,"Theo..MH...Da.":1283.64777,"DeltaM..ppm.":0.55,"Deltam.z..Da.":0.00036,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":4333220.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":59.05811,"Ion.Inject.Time..ms.":17.756,"RT..min.":7.6395,"First.Scan":5683,"Last.Scan":5683,"Master.Scan.s.":5682,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_05.raw","File.ID":"F17","Quan.Info":"","Peptides.Matched":580,"XCorr":2.21,"X..Protein.Groups":1,"Percolator.q.Value":0.00027,"Percolator.PEP":0.0002,"Percolator.SVMScore":1.322,"Precursor.Area":3062216.75,"Apex.RT..min.":7.64,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (T4)","Search.ID":"T","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LQETNPEEVPK","Annotated.Sequence":"[K].LQETNPEEVPK.[F]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5217,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":642.32771,"Contaminant":"False","Aligned.m.z..Da.":642.32779,"MH...Da.":1283.64814,"Theo..MH...Da.":1283.64777,"DeltaM..ppm.":0.29,"Deltam.z..Da.":0.00018,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":928686.9375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":29.85703,"Ion.Inject.Time..ms.":22,"RT..min.":7.6237,"First.Scan":5662,"Last.Scan":5662,"Master.Scan.s.":5656,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_05.raw","File.ID":"F17","Quan.Info":"","Peptides.Matched":558,"XCorr":2.3,"X..Protein.Groups":1,"Percolator.q.Value":0.00027,"Percolator.PEP":0.00024,"Percolator.SVMScore":1.29,"Precursor.Area":3062216.75,"Apex.RT..min.":7.64,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (T4)","Search.ID":"T","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSSPLHRLSPTGK","Annotated.Sequence":"[R].mSsPLHRLsPTGK.[S]","Modifications":"M1(Oxidation); S3(Phospho); S9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5858,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":793.84374,"Contaminant":"False","Aligned.m.z..Da.":793.84384,"MH...Da.":1586.6802,"Theo..MH...Da.":1586.67977,"DeltaM..ppm.":0.27,"Deltam.z..Da.":0.00022,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":822345.375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":60.22176,"Ion.Inject.Time..ms.":22,"RT..min.":7.7255,"First.Scan":5806,"Last.Scan":5806,"Master.Scan.s.":5799,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_05.raw","File.ID":"F17","Quan.Info":"","Peptides.Matched":983,"XCorr":2.39,"X..Protein.Groups":1,"Percolator.q.Value":0.00027,"Percolator.PEP":0.00005,"Percolator.SVMScore":1.592,"Precursor.Area":790506.5625,"Apex.RT..min.":7.73,"ptmRS..Binomial.Peptide.Score":247.23,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(3): 304.10; y2+-H(3) O(4) P(11): 627.82; y2+- -2H(3) O(4) P -2H(3) O(4) P(11): 578.83","ptmRS..Best.Site.Probabilities":"S3(Phospho): 99.99; S9(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(3): 100.0; S(9): 100.0; T(11): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (W4)","Search.ID":"W","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LQETNPEEVPKFEK","Annotated.Sequence":"[K].LQETNPEEVPKFEK.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.4786,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":563.29093,"Contaminant":"False","Aligned.m.z..Da.":563.29098,"MH...Da.":1687.85824,"Theo..MH...Da.":1687.85374,"DeltaM..ppm.":2.66,"Deltam.z..Da.":0.0015,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1405956.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":67.36179,"Ion.Inject.Time..ms.":11.895,"RT..min.":9.326,"First.Scan":7494,"Last.Scan":7494,"Master.Scan.s.":7489,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_02.raw","File.ID":"F14","Quan.Info":"","Peptides.Matched":833,"XCorr":1.17,"X..Protein.Groups":1,"Percolator.q.Value":0.00027,"Percolator.PEP":0.0021,"Percolator.SVMScore":0.926,"Precursor.Area":856834.875,"Apex.RT..min.":9.32,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"IQDPQMTGYVSTR","Annotated.Sequence":"[R].IQDPQMtGYVsTR.[Y]","Modifications":"T7(Phospho); S11(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P32485","Master.Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","Protein.Accessions":"P32485","Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.0393,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":828.3302,"Contaminant":"False","Aligned.m.z..Da.":828.33025,"MH...Da.":1655.65313,"Theo..MH...Da.":1655.65362,"DeltaM..ppm.":-0.3,"Deltam.z..Da.":-0.00025,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3612028.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":19.00005,"Ion.Inject.Time..ms.":22,"RT..min.":9.8654,"First.Scan":8370,"Last.Scan":8370,"Master.Scan.s.":8366,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":752,"XCorr":2.8,"X..Protein.Groups":1,"Percolator.q.Value":0.00028,"Percolator.PEP":0.00094,"Percolator.SVMScore":1.147,"Precursor.Area":4938312.5,"Apex.RT..min.":9.84,"ptmRS..Binomial.Peptide.Score":373.83,"ptmRS..Isoform.Confidence.Probability":0.005,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(11): 1316.53; y1+-H(3) O(4) P(10): 1201.51; y1+-H(3) O(4) P(8): 976.40; y1+-H(3) O(4) P(7): 845.36; y2+-H(3) O(4) P(11): 658.77; y2+-H(3) O(4) P(10): 601.26; y2+-H(3) O(4) P(8): 488.70; y1+(4): 462.27; y1+(3): 363.20","ptmRS..Best.Site.Probabilities":"T7(Phospho): 100; Y9(Phospho): 99.53","ptmRS..Phospho.Site.Probabilities":"T(7): 100.0; Y(9): 99.5; S(11): 0.5; T(12): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LEAGLSDSK","Annotated.Sequence":"[K].LEAGLSDSK.[Q]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.4346,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":460.24015,"Contaminant":"False","Aligned.m.z..Da.":460.24015,"MH...Da.":919.47302,"Theo..MH...Da.":919.4731,"DeltaM..ppm.":-0.09,"Deltam.z..Da.":-0.00004,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":7431305,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":20.69536,"Ion.Inject.Time..ms.":13.119,"RT..min.":6.6143,"First.Scan":4002,"Last.Scan":4002,"Master.Scan.s.":3998,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":418,"XCorr":2.37,"X..Protein.Groups":1,"Percolator.q.Value":0.00028,"Percolator.PEP":0.0006,"Percolator.SVMScore":1.264,"Precursor.Area":3942674.75,"Apex.RT..min.":6.62,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LEAELAESK","Annotated.Sequence":"[K].LEAELAESK.[Q]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.4217,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":495.26061,"Contaminant":"False","Aligned.m.z..Da.":495.26063,"MH...Da.":989.51395,"Theo..MH...Da.":989.51497,"DeltaM..ppm.":-1.03,"Deltam.z..Da.":-0.00051,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":7916575,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":52.88607,"Ion.Inject.Time..ms.":22,"RT..min.":7.7753,"First.Scan":5448,"Last.Scan":5448,"Master.Scan.s.":5441,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":369,"XCorr":1.66,"X..Protein.Groups":1,"Percolator.q.Value":0.00028,"Percolator.PEP":0.00106,"Percolator.SVMScore":1.115,"Precursor.Area":5810614,"Apex.RT..min.":7.76,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"IAEFPEEGIDPSTYLNN","Annotated.Sequence":"[K].IAEFPEEGIDPSTYLNN.[-]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6353,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":954.94821,"Contaminant":"False","Aligned.m.z..Da.":954.9482,"MH...Da.":1908.88914,"Theo..MH...Da.":1908.88616,"DeltaM..ppm.":1.56,"Deltam.z..Da.":0.00149,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":450895.40625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":17.5247,"First.Scan":18179,"Last.Scan":18179,"Master.Scan.s.":18168,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":1654,"XCorr":2.55,"X..Protein.Groups":1,"Percolator.q.Value":0.00028,"Percolator.PEP":0.00126,"Percolator.SVMScore":1.069,"Precursor.Area":352750.03125,"Apex.RT..min.":17.53,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"KSDEPSRESTPVR","Annotated.Sequence":"[K].KSDEPsRESTPVR.[S]","Modifications":"S6(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q08421","Master.Protein.Descriptions":"Enhancer of translation termination 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ETT1 PE=1 SV=1","Protein.Accessions":"Q08421","Protein.Descriptions":"Enhancer of translation termination 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ETT1 PE=1 SV=1","X..Missed.Cleavages":2,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0469,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":523.2422,"Contaminant":"False","Aligned.m.z..Da.":523.24222,"MH...Da.":1567.71206,"Theo..MH...Da.":1567.71119,"DeltaM..ppm.":0.55,"Deltam.z..Da.":0.00029,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":4695711.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":60.35645,"Ion.Inject.Time..ms.":10.563,"RT..min.":4.7047,"First.Scan":1796,"Last.Scan":1796,"Master.Scan.s.":1788,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":1131,"XCorr":2.56,"X..Protein.Groups":1,"Percolator.q.Value":0.00028,"Percolator.PEP":0.00131,"Percolator.SVMScore":1.059,"Precursor.Area":4707908,"Apex.RT..min.":4.71,"ptmRS..Binomial.Peptide.Score":175.08,"ptmRS..Isoform.Confidence.Probability":0.985,"ptmRS..Isoform.Group.Report":"y2+-H(3) O(4) P(12): 671.32; y2+-H(3) O(4) P(11): 627.81; y2+-H(3) O(4) P(10): 570.29; y2+-H(3) O(4) P(9): 505.77; y2+(7): 422.74","ptmRS..Best.Site.Probabilities":"S6(Phospho): 98.51","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(6): 98.5; S(9): 0.8; T(10): 0.8"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (V4)","Search.ID":"V","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSSPLHRLSPTGK","Annotated.Sequence":"[R].mSsPLHRLsPTGK.[S]","Modifications":"M1(Oxidation); S3(Phospho); S9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5385,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":793.84304,"Contaminant":"False","Aligned.m.z..Da.":793.8432,"MH...Da.":1586.67881,"Theo..MH...Da.":1586.67977,"DeltaM..ppm.":-0.6,"Deltam.z..Da.":-0.00048,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1800179.625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":7.33989,"Ion.Inject.Time..ms.":22,"RT..min.":7.7414,"First.Scan":5588,"Last.Scan":5588,"Master.Scan.s.":5582,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_03.raw","File.ID":"F15","Quan.Info":"","Peptides.Matched":979,"XCorr":2.21,"X..Protein.Groups":1,"Percolator.q.Value":0.00028,"Percolator.PEP":0.00016,"Percolator.SVMScore":1.272,"Precursor.Area":1353394.125,"Apex.RT..min.":7.74,"ptmRS..Binomial.Peptide.Score":267.35,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(3): 304.10; y2+-H(3) O(4) P(11): 627.82; y2+- -2H(3) O(4) P -2H(3) O(4) P(11): 578.83","ptmRS..Best.Site.Probabilities":"S3(Phospho): 100; S9(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(3): 100.0; S(9): 100.0; T(11): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (V4)","Search.ID":"V","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSSPLHRLSPTGK","Annotated.Sequence":"[R].mSsPLHRLsPTGK.[S]","Modifications":"M1(Oxidation); S3(Phospho); S9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.3841,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":529.56474,"Contaminant":"False","Aligned.m.z..Da.":529.56484,"MH...Da.":1586.67967,"Theo..MH...Da.":1586.67977,"DeltaM..ppm.":-0.06,"Deltam.z..Da.":-0.00003,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2487375.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":27.45494,"Ion.Inject.Time..ms.":22,"RT..min.":7.7228,"First.Scan":5562,"Last.Scan":5562,"Master.Scan.s.":5556,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_03.raw","File.ID":"F15","Quan.Info":"","Peptides.Matched":984,"XCorr":3.28,"X..Protein.Groups":1,"Percolator.q.Value":0.00028,"Percolator.PEP":0.00006,"Percolator.SVMScore":1.455,"Precursor.Area":3342271.75,"Apex.RT..min.":7.74,"ptmRS..Binomial.Peptide.Score":273.19,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(8): 877.50; y2+-H(3) O(4) P(11): 627.82; y2+-H(3) O(4) P(10): 544.32; y2+-H(3) O(4) P(9): 495.80; y2+-H(3) O(4) P(8): 439.25; y1+(4): 402.23; y1+(3): 305.18","ptmRS..Best.Site.Probabilities":"S3(Phospho): 100; S9(Phospho): 99.99","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(3): 100.0; S(9): 100.0; T(11): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (V4)","Search.ID":"V","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"IQDPQMTGYVSTR","Annotated.Sequence":"[R].IQDPQmtGYVsTR.[Y]","Modifications":"M6(Oxidation); T7(Phospho); S11(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P32485","Master.Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","Protein.Accessions":"P32485","Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.0532,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":836.32745,"Contaminant":"False","Aligned.m.z..Da.":836.32762,"MH...Da.":1671.64762,"Theo..MH...Da.":1671.64853,"DeltaM..ppm.":-0.55,"Deltam.z..Da.":-0.00046,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2121527.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":17.97764,"Ion.Inject.Time..ms.":22,"RT..min.":7.7612,"First.Scan":5616,"Last.Scan":5616,"Master.Scan.s.":5608,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_03.raw","File.ID":"F15","Quan.Info":"","Peptides.Matched":753,"XCorr":2.63,"X..Protein.Groups":1,"Percolator.q.Value":0.00028,"Percolator.PEP":0.00006,"Percolator.SVMScore":1.454,"Precursor.Area":1500608.75,"Apex.RT..min.":7.75,"ptmRS..Binomial.Peptide.Score":336.65,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(11): 1332.53; y1+-H(3) O(4) P(10): 1217.50; y1+-H(3) O(4) P(8): 992.39; y1+-H(3) O(4) P(7): 845.36; y2+-H(3) O(4) P(11): 666.77; y2+-H(3) O(4) P(10): 609.25; y1+(4): 462.27; y1+(3): 363.20","ptmRS..Best.Site.Probabilities":"T7(Phospho): 100; Y9(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"T(7): 100.0; Y(9): 100.0; S(11): 0.0; T(12): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (V4)","Search.ID":"V","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"HSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[R].HSDsYsENETNHTNVPISSTGGTNNK.[T]","Modifications":"S4(Phospho); S6(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.181,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":984.0555,"Contaminant":"False","Aligned.m.z..Da.":984.05571,"MH...Da.":2950.15196,"Theo..MH...Da.":2950.15241,"DeltaM..ppm.":-0.15,"Deltam.z..Da.":-0.00015,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1535899.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":8.0244,"First.Scan":5967,"Last.Scan":5967,"Master.Scan.s.":5959,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_03.raw","File.ID":"F15","Quan.Info":"","Peptides.Matched":1106,"XCorr":6.63,"X..Protein.Groups":1,"Percolator.q.Value":0.00028,"Percolator.PEP":2.382e-10,"Percolator.SVMScore":3.713,"Precursor.Area":2819132.5,"Apex.RT..min.":8.03,"ptmRS..Binomial.Peptide.Score":575.44,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(4): 409.15; b1+-H(3) O(4) P(5): 572.21; b1+-H(3) O(4) P(6): 739.21; b1+-H(3) O(4) P(9): 1111.34; b1+-H(3) O(4) P(11): 1326.43; b2+-H(3) O(4) P(12): 732.25; b2+-H(3) O(4) P(14): 839.79; b2+-H(3) O(4) P(15): 889.33; y2+- -2H(3) O(4) P -2H(3) O(4) P(24): 1265.56; b1+(2): 225.10; b1+(3): 340.13","ptmRS..Best.Site.Probabilities":"S4(Phospho): 100; S6(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(4): 100.0; Y(5): 0.0; S(6): 100.0; T(10): 0.0; T(13): 0.0; S(18): 0.0; S(19): 0.0; T(20): 0.0; T(23): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (V4)","Search.ID":"V","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"HSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[R].HSDSySENETNHTNVPISSTGGTNNK.[T]","Modifications":"Y5(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":0,"Charge":4,"Original.Precursor.Charge":4,"DeltaScore":0.0068,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":718.30159,"Contaminant":"False","Aligned.m.z..Da.":718.30173,"MH...Da.":2870.18454,"Theo..MH...Da.":2870.18608,"DeltaM..ppm.":-0.54,"Deltam.z..Da.":-0.00038,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2890653.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":2.29257,"Ion.Inject.Time..ms.":22,"RT..min.":7.6578,"First.Scan":5468,"Last.Scan":5468,"Master.Scan.s.":5465,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_03.raw","File.ID":"F15","Quan.Info":"","Peptides.Matched":2484,"XCorr":5.86,"X..Protein.Groups":1,"Percolator.q.Value":0.00028,"Percolator.PEP":1.635e-10,"Percolator.SVMScore":3.781,"Precursor.Area":3345615.75,"Apex.RT..min.":7.66,"ptmRS..Binomial.Peptide.Score":307.03,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(10): 1132.42; b1+-H(3) O(4) P(11): 1246.46; b1+-H(3) O(4) P(12): 1383.52; b2+-H(3) O(4) P(7): 394.65; b2+-H(3) O(4) P(13): 742.79; b2+-H(3) O(4) P(14): 799.81; b2+-H(3) O(4) P(15): 849.34; b2+-H(3) O(4) P(17): 954.41; b1+(4): 427.16","ptmRS..Best.Site.Probabilities":"S6(Phospho): 99.37","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(4): 0.6; Y(5): 0.0; S(6): 99.4; T(10): 0.0; T(13): 0.0; S(18): 0.0; S(19): 0.0; T(20): 0.0; T(23): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (V4)","Search.ID":"V","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"HSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[R].HSDSYsENETNHTNVPISSTGGTNNK.[T]","Modifications":"S6(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.006,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":957.4005,"Contaminant":"False","Aligned.m.z..Da.":957.40068,"MH...Da.":2870.18695,"Theo..MH...Da.":2870.18608,"DeltaM..ppm.":0.3,"Deltam.z..Da.":0.00029,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1195900.625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":7.651,"First.Scan":5459,"Last.Scan":5459,"Master.Scan.s.":5452,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_03.raw","File.ID":"F15","Quan.Info":"","Peptides.Matched":2506,"XCorr":6.67,"X..Protein.Groups":1,"Percolator.q.Value":0.00028,"Percolator.PEP":6.093e-14,"Percolator.SVMScore":5.216,"Precursor.Area":7766172,"Apex.RT..min.":7.66,"ptmRS..Binomial.Peptide.Score":544.61,"ptmRS..Isoform.Confidence.Probability":0.006,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(4): 409.15; b1+-H(3) O(4) P(5): 572.21; b1+-H(3) O(4) P(6): 659.24; b1+-H(3) O(4) P(9): 1031.37; b1+-H(3) O(4) P(11): 1246.46; b1+-H(3) O(4) P(12): 1383.52; b1+-H(3) O(4) P(15): 1697.68; b2+-H(3) O(4) P(12): 692.26; b2+-H(3) O(4) P(13): 742.79; b2+-H(3) O(4) P(14): 799.81; b2+-H(3) O(4) P(15): 849.34; y2+-H(3) O(4) P(25): 1318.08; y2+-H(3) O(4) P(24): 1274.56; y2+-H(3) O(4) P(23): 1217.05","ptmRS..Best.Site.Probabilities":"S4(Phospho): 99.4","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(4): 99.4; Y(5): 0.0; S(6): 0.6; T(10): 0.0; T(13): 0.0; S(18): 0.0; S(19): 0.0; T(20): 0.0; T(23): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (V4)","Search.ID":"V","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LQETNPEEVPK","Annotated.Sequence":"[K].LQETNPEEVPK.[F]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5647,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":642.32798,"Contaminant":"False","Aligned.m.z..Da.":642.32811,"MH...Da.":1283.64869,"Theo..MH...Da.":1283.64777,"DeltaM..ppm.":0.72,"Deltam.z..Da.":0.00046,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2361306.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":3.64296,"Ion.Inject.Time..ms.":22,"RT..min.":7.6403,"First.Scan":5443,"Last.Scan":5443,"Master.Scan.s.":5439,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_03.raw","File.ID":"F15","Quan.Info":"","Peptides.Matched":573,"XCorr":2.32,"X..Protein.Groups":1,"Percolator.q.Value":0.00028,"Percolator.PEP":0.00007,"Percolator.SVMScore":1.412,"Precursor.Area":4396616.5,"Apex.RT..min.":7.66,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (V4)","Search.ID":"V","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"IQDPQMTGYVSTR","Annotated.Sequence":"[R].IQDPQmTGyVSTR.[Y]","Modifications":"M6(Oxidation); Y9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P32485","Master.Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","Protein.Accessions":"P32485","Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1313,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":796.34367,"Contaminant":"False","Aligned.m.z..Da.":796.34383,"MH...Da.":1591.68008,"Theo..MH...Da.":1591.6822,"DeltaM..ppm.":-1.33,"Deltam.z..Da.":-0.00106,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2285656.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":7.6685,"First.Scan":5484,"Last.Scan":5484,"Master.Scan.s.":5478,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_03.raw","File.ID":"F15","Quan.Info":"","Peptides.Matched":1149,"XCorr":2.97,"X..Protein.Groups":1,"Percolator.q.Value":0.00028,"Percolator.PEP":1.496e-06,"Percolator.SVMScore":2.123,"Precursor.Area":3628071.25,"Apex.RT..min.":7.69,"ptmRS..Binomial.Peptide.Score":279.66,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+(6): 762.32; y1+(5): 705.30","ptmRS..Best.Site.Probabilities":"Y9(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"T(7): 0.0; Y(9): 100.0; S(11): 0.0; T(12): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (V4)","Search.ID":"V","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"IGNNELLHR","Annotated.Sequence":"[K].IGNNELLHR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q03558","Master.Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","Protein.Accessions":"Q03558","Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.3497,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":355.86487,"Contaminant":"False","Aligned.m.z..Da.":355.86494,"MH...Da.":1065.58007,"Theo..MH...Da.":1065.57997,"DeltaM..ppm.":0.09,"Deltam.z..Da.":0.00003,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1254819.125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":12.30877,"Ion.Inject.Time..ms.":22,"RT..min.":7.5815,"First.Scan":5358,"Last.Scan":5358,"Master.Scan.s.":5348,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_03.raw","File.ID":"F15","Quan.Info":"","Peptides.Matched":300,"XCorr":1.63,"X..Protein.Groups":1,"Percolator.q.Value":0.00028,"Percolator.PEP":0.00048,"Percolator.SVMScore":1.072,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (V4)","Search.ID":"V","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"AAEAGETGAATSATEGDNNNNTAAGDKK","Annotated.Sequence":"[K].AAEAGETGAATsATEGDNNNNTAAGDKK.[G]","Modifications":"S12(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0119,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":906.04951,"Contaminant":"False","Aligned.m.z..Da.":906.04946,"MH...Da.":2716.13398,"Theo..MH...Da.":2716.13298,"DeltaM..ppm.":0.37,"Deltam.z..Da.":0.00033,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2861315.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":3.66282,"Ion.Inject.Time..ms.":22,"RT..min.":5.0227,"First.Scan":1792,"Last.Scan":1792,"Master.Scan.s.":1786,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_03.raw","File.ID":"F15","Quan.Info":"","Peptides.Matched":2560,"XCorr":5.88,"X..Protein.Groups":1,"Percolator.q.Value":0.00028,"Percolator.PEP":9.753e-16,"Percolator.SVMScore":6.397,"ptmRS..Binomial.Peptide.Score":539.02,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(17): 1688.75; y2+-H(3) O(4) P(24): 1138.50; y2+-H(3) O(4) P(22): 1045.47; y2+-H(3) O(4) P(18): 895.40; y2+-H(3) O(4) P(17): 844.88; y1+(17): 1786.72","ptmRS..Best.Site.Probabilities":"S12(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"T(7): 0.0; T(11): 0.0; S(12): 100.0; T(14): 0.0; T(22): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (V4)","Search.ID":"V","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MFILDEADEMLSSGFK","Annotated.Sequence":"[K].mFILDEADEmLSSGFK.[E]","Modifications":"M1(Oxidation); M10(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6849,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":932.92021,"Contaminant":"False","Aligned.m.z..Da.":932.92013,"MH...Da.":1864.83315,"Theo..MH...Da.":1864.83433,"DeltaM..ppm.":-0.63,"Deltam.z..Da.":-0.00059,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":472771.71875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":11.58726,"Ion.Inject.Time..ms.":22,"RT..min.":16.6905,"First.Scan":16826,"Last.Scan":16826,"Master.Scan.s.":16823,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_03.raw","File.ID":"F15","Quan.Info":"","Peptides.Matched":1892,"XCorr":3.11,"X..Protein.Groups":1,"Percolator.q.Value":0.00028,"Percolator.PEP":3.542e-08,"Percolator.SVMScore":2.803,"Precursor.Area":387111.09375,"Apex.RT..min.":16.7,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (V4)","Search.ID":"V","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ILISTDLLAR","Annotated.Sequence":"[R].ILISTDLLAR.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5129,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":557.84566,"Contaminant":"False","Aligned.m.z..Da.":557.84565,"MH...Da.":1114.68404,"Theo..MH...Da.":1114.68304,"DeltaM..ppm.":0.9,"Deltam.z..Da.":0.0005,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1326413,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":16.45105,"Ion.Inject.Time..ms.":22,"RT..min.":15.5924,"First.Scan":15466,"Last.Scan":15466,"Master.Scan.s.":15456,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_03.raw","File.ID":"F15","Quan.Info":"","Peptides.Matched":124,"XCorr":2.71,"X..Protein.Groups":1,"Percolator.q.Value":0.00028,"Percolator.PEP":0.00007,"Percolator.SVMScore":1.427,"Precursor.Area":1382029.375,"Apex.RT..min.":15.6,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (V4)","Search.ID":"V","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MGSINNNFNTNNNSNTDLDRDWK","Annotated.Sequence":"[-].mGSINNNFNTNNNsNTDLDRDWK.[T]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S14(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P39517","Master.Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","Protein.Accessions":"P39517","Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0571,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":892.37261,"Contaminant":"False","Aligned.m.z..Da.":892.3727,"MH...Da.":2675.10327,"Theo..MH...Da.":2675.11179,"DeltaM..ppm.":-3.18,"Deltam.z..Da.":-0.00284,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":231117.65625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":13.37536,"Ion.Inject.Time..ms.":22,"RT..min.":13.5502,"First.Scan":12983,"Last.Scan":12983,"Master.Scan.s.":12979,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_03.raw","File.ID":"F15","Quan.Info":"","Peptides.Matched":2089,"XCorr":4.38,"X..Protein.Groups":1,"Percolator.q.Value":0.00028,"Percolator.PEP":8.572e-08,"Percolator.SVMScore":2.643,"Precursor.Area":3279502.25,"Apex.RT..min.":13.58,"ptmRS..Binomial.Peptide.Score":439.44,"ptmRS..Isoform.Confidence.Probability":0.994,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(12): 1459.66; y1+-H(3) O(4) P(11): 1345.61; y1+-H(3) O(4) P(10): 1231.57; y2+-H(3) O(4) P(17): 1025.45; y2+-H(3) O(4) P(16): 968.43; y2+-H(3) O(4) P(15): 894.90; y2+-H(3) O(4) P(14): 837.88; y2+-H(3) O(4) P(13): 787.35; y2+-H(3) O(4) P(12): 730.33; y2+-H(3) O(4) P(10): 616.29; y1+(9): 1162.55","ptmRS..Best.Site.Probabilities":"S14(Phospho): 99.36","ptmRS..Phospho.Site.Probabilities":"S(3): 0.0; T(10): 0.0; S(14): 99.4; T(16): 0.6"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (V4)","Search.ID":"V","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].mSEGItDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); T6(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.115,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1096.46754,"Contaminant":"False","Aligned.m.z..Da.":1096.46753,"MH...Da.":2191.92782,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":-0.01,"Deltam.z..Da.":-1e-05,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":277228.0625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":15.5332,"First.Scan":15399,"Last.Scan":15399,"Master.Scan.s.":15388,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_03.raw","File.ID":"F15","Quan.Info":"","Peptides.Matched":1962,"XCorr":4.61,"X..Protein.Groups":1,"Percolator.q.Value":0.00028,"Percolator.PEP":1.706e-09,"Percolator.SVMScore":3.355,"Precursor.Area":256361.5625,"Apex.RT..min.":15.53,"ptmRS..Binomial.Peptide.Score":434.61,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(6): 512.24; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+-H(3) O(4) P(9): 869.39; b1+-H(3) O(4) P(10): 998.43; y1+-H(3) O(4) P(14): 1665.76; b1+(3): 259.09; b1+(4): 316.11; b1+(5): 429.20","ptmRS..Best.Site.Probabilities":"T6(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; T(6): 100.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (V4)","Search.ID":"V","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].mSEGItDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); T6(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0921,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":731.31452,"Contaminant":"False","Aligned.m.z..Da.":731.31451,"MH...Da.":2191.92902,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":0.54,"Deltam.z..Da.":0.00039,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":240297.59375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":16.18196,"Ion.Inject.Time..ms.":22,"RT..min.":15.5378,"First.Scan":15403,"Last.Scan":15403,"Master.Scan.s.":15401,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_03.raw","File.ID":"F15","Quan.Info":"","Peptides.Matched":1989,"XCorr":2.28,"X..Protein.Groups":1,"Percolator.q.Value":0.00028,"Percolator.PEP":0.00004,"Percolator.SVMScore":1.532,"Precursor.Area":145105.8125,"Apex.RT..min.":15.54,"ptmRS..Binomial.Peptide.Score":243.67,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+(2): 130.05; b1+(3): 259.09; b1+(4): 316.11; b2+(3): 130.05","ptmRS..Best.Site.Probabilities":"T6(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; T(6): 100.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (V4)","Search.ID":"V","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEySDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); Y12(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.3858,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40713,"Contaminant":"False","Aligned.m.z..Da.":967.40712,"MH...Da.":2900.20686,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":2.6,"Deltam.z..Da.":0.00251,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":365882.90625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":6.94648,"Ion.Inject.Time..ms.":22,"RT..min.":15.5372,"First.Scan":15402,"Last.Scan":15402,"Master.Scan.s.":15401,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_03.raw","File.ID":"F15","Quan.Info":"","Peptides.Matched":2587,"XCorr":4.38,"X..Protein.Groups":1,"Percolator.q.Value":0.00028,"Percolator.PEP":4.855e-08,"Percolator.SVMScore":2.746,"ptmRS..Binomial.Peptide.Score":321.73,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(14): 1621.73; y2+-H(3) O(4) P(14): 811.37; b1+(12): 1344.56","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (V4)","Search.ID":"V","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"TGTFSIAALQR","Annotated.Sequence":"[K].TGTFSIAALQR.[I]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7699,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":582.82227,"Contaminant":"False","Aligned.m.z..Da.":582.82233,"MH...Da.":1164.63727,"Theo..MH...Da.":1164.63715,"DeltaM..ppm.":0.1,"Deltam.z..Da.":0.00006,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1012197.1875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":9.32344,"Ion.Inject.Time..ms.":22,"RT..min.":13.3711,"First.Scan":12760,"Last.Scan":12760,"Master.Scan.s.":12758,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_03.raw","File.ID":"F15","Quan.Info":"","Peptides.Matched":361,"XCorr":3.39,"X..Protein.Groups":1,"Percolator.q.Value":0.00028,"Percolator.PEP":6.162e-07,"Percolator.SVMScore":2.284,"Precursor.Area":1105150.125,"Apex.RT..min.":13.38,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (V4)","Search.ID":"V","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FDDMELDENLLR","Annotated.Sequence":"[K].FDDMELDENLLR.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7087,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":755.34755,"Contaminant":"False","Aligned.m.z..Da.":755.34754,"MH...Da.":1509.68784,"Theo..MH...Da.":1509.68898,"DeltaM..ppm.":-0.76,"Deltam.z..Da.":-0.00057,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":318874.875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":24.22959,"Ion.Inject.Time..ms.":22,"RT..min.":16.042,"First.Scan":16027,"Last.Scan":16027,"Master.Scan.s.":16015,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_03.raw","File.ID":"F15","Quan.Info":"","Peptides.Matched":1081,"XCorr":2.3,"X..Protein.Groups":1,"Percolator.q.Value":0.00028,"Percolator.PEP":0.00002,"Percolator.SVMScore":1.684,"Precursor.Area":354365.15625,"Apex.RT..min.":16.05,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (V4)","Search.ID":"V","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.4274,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40745,"Contaminant":"False","Aligned.m.z..Da.":967.40743,"MH...Da.":2900.2078,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":2.92,"Deltam.z..Da.":0.00282,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":180201.57812,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":16.0453,"First.Scan":16029,"Last.Scan":16029,"Master.Scan.s.":16028,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_03.raw","File.ID":"F15","Quan.Info":"","Peptides.Matched":2624,"XCorr":3.72,"X..Protein.Groups":1,"Percolator.q.Value":0.00028,"Percolator.PEP":2.504e-06,"Percolator.SVMScore":2.029,"ptmRS..Binomial.Peptide.Score":338.31,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b2+-H(3) O(4) P(18): 1014.90; y2+-H(3) O(4) P(14): 811.37","ptmRS..Best.Site.Probabilities":"S13(Phospho): 99.99","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (V4)","Search.ID":"V","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"AIMPIIEGHDVLAQAQSGTGK","Annotated.Sequence":"[R].AImPIIEGHDVLAQAQSGTGK.[T]","Modifications":"M3(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.7136,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":718.04096,"Contaminant":"False","Aligned.m.z..Da.":718.041,"MH...Da.":2152.10833,"Theo..MH...Da.":2152.10668,"DeltaM..ppm.":0.77,"Deltam.z..Da.":0.00055,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":493387.625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":3.80312,"Ion.Inject.Time..ms.":22,"RT..min.":12.7642,"First.Scan":11993,"Last.Scan":11993,"Master.Scan.s.":11991,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_03.raw","File.ID":"F15","Quan.Info":"","Peptides.Matched":1001,"XCorr":4.05,"X..Protein.Groups":1,"Percolator.q.Value":0.00028,"Percolator.PEP":5.898e-11,"Percolator.SVMScore":3.966,"Precursor.Area":686256,"Apex.RT..min.":12.77,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (V4)","Search.ID":"V","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"APQALMLAPTR","Annotated.Sequence":"[K].APQALMLAPTR.[E]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6418,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":584.82909,"Contaminant":"False","Aligned.m.z..Da.":584.8291,"MH...Da.":1168.65091,"Theo..MH...Da.":1168.65069,"DeltaM..ppm.":0.19,"Deltam.z..Da.":0.00011,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":467615.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":62.87975,"Ion.Inject.Time..ms.":22,"RT..min.":11.6327,"First.Scan":10455,"Last.Scan":10455,"Master.Scan.s.":10444,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_03.raw","File.ID":"F15","Quan.Info":"","Peptides.Matched":162,"XCorr":2.82,"X..Protein.Groups":1,"Percolator.q.Value":0.00028,"Percolator.PEP":4.991e-07,"Percolator.SVMScore":2.322,"Precursor.Area":381674.9375,"Apex.RT..min.":11.63,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (V4)","Search.ID":"V","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FDDMELDENLLR","Annotated.Sequence":"[K].FDDmELDENLLR.[G]","Modifications":"M4(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6135,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":763.34389,"Contaminant":"False","Aligned.m.z..Da.":763.34391,"MH...Da.":1525.68051,"Theo..MH...Da.":1525.6839,"DeltaM..ppm.":-2.22,"Deltam.z..Da.":-0.0017,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":572145.375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":80.21812,"Ion.Inject.Time..ms.":22,"RT..min.":14.4759,"First.Scan":14130,"Last.Scan":14130,"Master.Scan.s.":14124,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_03.raw","File.ID":"F15","Quan.Info":"","Peptides.Matched":965,"XCorr":2.07,"X..Protein.Groups":1,"Percolator.q.Value":0.00028,"Percolator.PEP":3.512e-06,"Percolator.SVMScore":1.968,"Precursor.Area":760644.875,"Apex.RT..min.":14.49,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (V4)","Search.ID":"V","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"APQALMLAPTR","Annotated.Sequence":"[K].APQALMLAPTR.[E]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":3,"DeltaScore":0.3043,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":584.82757,"Contaminant":"False","Aligned.m.z..Da.":584.82758,"MH...Da.":1168.64786,"Theo..MH...Da.":1168.65069,"DeltaM..ppm.":-2.42,"Deltam.z..Da.":-0.00141,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":446479.59375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":79.30086,"Ion.Inject.Time..ms.":22,"RT..min.":11.6511,"First.Scan":10481,"Last.Scan":10481,"Master.Scan.s.":10470,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_03.raw","File.ID":"F15","Quan.Info":"","Peptides.Matched":225,"XCorr":1.38,"X..Protein.Groups":1,"Percolator.q.Value":0.00028,"Percolator.PEP":0.00017,"Percolator.SVMScore":1.261,"Precursor.Area":381674.9375,"Apex.RT..min.":11.63,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (V4)","Search.ID":"V","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KFQDDTLNR","Annotated.Sequence":"[K].KFQDDtLNR.[V]","Modifications":"T6(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6797,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":608.77248,"Contaminant":"False","Aligned.m.z..Da.":608.77247,"MH...Da.":1216.53768,"Theo..MH...Da.":1216.53579,"DeltaM..ppm.":1.55,"Deltam.z..Da.":0.00094,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3059766.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":17.94352,"Ion.Inject.Time..ms.":22,"RT..min.":6.2094,"First.Scan":3140,"Last.Scan":3140,"Master.Scan.s.":3138,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_03.raw","File.ID":"F15","Quan.Info":"","Peptides.Matched":612,"XCorr":3.06,"X..Protein.Groups":1,"Percolator.q.Value":0.00028,"Percolator.PEP":0.00022,"Percolator.SVMScore":1.212,"Precursor.Area":1540967.5,"Apex.RT..min.":6.21,"ptmRS..Binomial.Peptide.Score":369.67,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"T6(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"T(6): 100.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (V4)","Search.ID":"V","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"APQALMLAPTR","Annotated.Sequence":"[K].APQALMLAPTR.[E]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":3,"DeltaScore":0.6094,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":584.83037,"Contaminant":"False","Aligned.m.z..Da.":584.83038,"MH...Da.":1168.65347,"Theo..MH...Da.":1168.65069,"DeltaM..ppm.":2.38,"Deltam.z..Da.":0.00139,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":296296.40625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":70.98322,"Ion.Inject.Time..ms.":22,"RT..min.":11.6148,"First.Scan":10430,"Last.Scan":10430,"Master.Scan.s.":10418,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_03.raw","File.ID":"F15","Quan.Info":"","Peptides.Matched":178,"XCorr":2.97,"X..Protein.Groups":1,"Percolator.q.Value":0.00028,"Percolator.PEP":1.768e-06,"Percolator.SVMScore":2.092,"Precursor.Area":381674.9375,"Apex.RT..min.":11.63,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (V4)","Search.ID":"V","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MGSINNNFNTNNNSNTDLDRDWK","Annotated.Sequence":"[-].mGSINNnFNTNNNsNTDLDRDWK.[T]","Modifications":"N-Term(Prot)(Met-loss); N7(Deamidated); S14(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P39517","Master.Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","Protein.Accessions":"P39517","Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0231,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":878.70385,"Contaminant":"False","Aligned.m.z..Da.":878.70388,"MH...Da.":2634.09699,"Theo..MH...Da.":2634.08524,"DeltaM..ppm.":4.46,"Deltam.z..Da.":0.00392,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":381870.1875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":11.3082,"First.Scan":9999,"Last.Scan":9999,"Master.Scan.s.":9989,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_03.raw","File.ID":"F15","Quan.Info":"NoneMonoisotopic","Peptides.Matched":2174,"XCorr":4.32,"X..Protein.Groups":1,"Percolator.q.Value":0.00028,"Percolator.PEP":1.719e-08,"Percolator.SVMScore":2.935,"ptmRS..Binomial.Peptide.Score":365.11,"ptmRS..Isoform.Confidence.Probability":0.992,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(11): 1345.61; y1+-H(3) O(4) P(10): 1231.57; y2+-H(3) O(4) P(19): 1139.99; y2+-H(3) O(4) P(18): 1082.97; y2+-H(3) O(4) P(17): 1025.95; y2+-H(3) O(4) P(16): 968.43; y2+-H(3) O(4) P(15): 894.90; y2+-H(3) O(4) P(13): 787.35; y2+-H(3) O(4) P(12): 730.33; y2+-H(3) O(4) P(11): 673.31; y1+(9): 1162.55","ptmRS..Best.Site.Probabilities":"S14(Phospho): 99.17","ptmRS..Phospho.Site.Probabilities":"S(3): 0.0; T(10): 0.0; S(14): 99.2; T(16): 0.8"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (V4)","Search.ID":"V","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].msEGITDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S2(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":3,"DeltaScore":0.134,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1096.46703,"Contaminant":"False","Aligned.m.z..Da.":1096.46702,"MH...Da.":2191.92679,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":-0.48,"Deltam.z..Da.":-0.00053,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":4290054,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":69.37107,"Ion.Inject.Time..ms.":22,"RT..min.":15.7677,"First.Scan":15681,"Last.Scan":15681,"Master.Scan.s.":15672,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_03.raw","File.ID":"F15","Quan.Info":"","Peptides.Matched":1964,"XCorr":3.73,"X..Protein.Groups":1,"Percolator.q.Value":0.00028,"Percolator.PEP":2.188e-07,"Percolator.SVMScore":2.472,"Precursor.Area":8483639,"Apex.RT..min.":15.79,"ptmRS..Binomial.Peptide.Score":326.06,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(2): 112.04; b1+-H(3) O(4) P(3): 241.08; b1+-H(3) O(4) P(4): 298.10; b1+-H(3) O(4) P(5): 411.19; b1+-H(3) O(4) P(6): 512.24; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+-H(3) O(4) P(9): 869.39","ptmRS..Best.Site.Probabilities":"S2(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 100.0; T(6): 0.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (V4)","Search.ID":"V","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"TAVFYGGTPISK","Annotated.Sequence":"[K].TAVFYGGTPISK.[D]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5259,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":620.83163,"Contaminant":"False","Aligned.m.z..Da.":620.83166,"MH...Da.":1240.65598,"Theo..MH...Da.":1240.65721,"DeltaM..ppm.":-1,"Deltam.z..Da.":-0.00062,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":353916.3125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":71.79472,"Ion.Inject.Time..ms.":22,"RT..min.":11.1177,"First.Scan":9731,"Last.Scan":9731,"Master.Scan.s.":9729,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_03.raw","File.ID":"F15","Quan.Info":"","Peptides.Matched":417,"XCorr":2.51,"X..Protein.Groups":1,"Percolator.q.Value":0.00028,"Percolator.PEP":0.00026,"Percolator.SVMScore":1.184,"Precursor.Area":326990.5625,"Apex.RT..min.":11.13,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (V4)","Search.ID":"V","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KDELTLEGIK","Annotated.Sequence":"[K].KDELTLEGIK.[Q]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.5441,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":382.55204,"Contaminant":"False","Aligned.m.z..Da.":382.55207,"MH...Da.":1145.64159,"Theo..MH...Da.":1145.64123,"DeltaM..ppm.":0.31,"Deltam.z..Da.":0.00012,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1713657.875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":43.35657,"Ion.Inject.Time..ms.":22,"RT..min.":10.2634,"First.Scan":8607,"Last.Scan":8607,"Master.Scan.s.":8598,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_03.raw","File.ID":"F15","Quan.Info":"","Peptides.Matched":307,"XCorr":2.72,"X..Protein.Groups":1,"Percolator.q.Value":0.00028,"Percolator.PEP":0.00008,"Percolator.SVMScore":1.397,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (V4)","Search.ID":"V","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0545,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40774,"Contaminant":"False","Aligned.m.z..Da.":967.40773,"MH...Da.":2900.20867,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":3.22,"Deltam.z..Da.":0.00311,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":256667.35938,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":15.0222,"First.Scan":14762,"Last.Scan":14762,"Master.Scan.s.":14756,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_03.raw","File.ID":"F15","Quan.Info":"","Peptides.Matched":2626,"XCorr":4.59,"X..Protein.Groups":1,"Percolator.q.Value":0.00028,"Percolator.PEP":2.889e-10,"Percolator.SVMScore":3.678,"Precursor.Area":365911.125,"Apex.RT..min.":14.82,"ptmRS..Binomial.Peptide.Score":454.61,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b2+-H(3) O(4) P(18): 1014.90; y2+-H(3) O(4) P(14): 811.37; b1+(12): 1344.56","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (V4)","Search.ID":"V","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":5,"DeltaScore":0.058,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40512,"Contaminant":"False","Aligned.m.z..Da.":967.40511,"MH...Da.":2900.2008,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":0.51,"Deltam.z..Da.":0.00049,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":5298504.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":11.04195,"Ion.Inject.Time..ms.":22,"RT..min.":15.1235,"First.Scan":14889,"Last.Scan":14889,"Master.Scan.s.":14886,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_03.raw","File.ID":"F15","Quan.Info":"","Peptides.Matched":2509,"XCorr":7.42,"X..Protein.Groups":1,"Percolator.q.Value":0.00028,"Percolator.PEP":1.199e-15,"Percolator.SVMScore":6.2,"Precursor.Area":21028214,"Apex.RT..min.":15.08,"ptmRS..Binomial.Peptide.Score":717.65,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(13): 1413.58; b1+-H(3) O(4) P(14): 1528.61; b1+-H(3) O(4) P(15): 1642.65; y1+-H(3) O(4) P(15): 1750.78; y1+-H(3) O(4) P(14): 1621.73; b2+-H(3) O(4) P(16): 886.35; b2+-H(3) O(4) P(18): 1014.90; y2+-H(3) O(4) P(14): 811.37; y2+-H(3) O(4) P(13): 729.84; b1+(12): 1344.56; y1+(13): 1556.65","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (V4)","Search.ID":"V","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ILISTDLLAR","Annotated.Sequence":"[R].ILISTDLLAR.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.545,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":557.84578,"Contaminant":"False","Aligned.m.z..Da.":557.84577,"MH...Da.":1114.68428,"Theo..MH...Da.":1114.68304,"DeltaM..ppm.":1.12,"Deltam.z..Da.":0.00062,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":389624.09375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":42.48408,"Ion.Inject.Time..ms.":22,"RT..min.":15.6314,"First.Scan":15511,"Last.Scan":15511,"Master.Scan.s.":15510,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_03.raw","File.ID":"F15","Quan.Info":"","Peptides.Matched":124,"XCorr":2.11,"X..Protein.Groups":1,"Percolator.q.Value":0.00028,"Percolator.PEP":0.00038,"Percolator.SVMScore":1.113,"Precursor.Area":1382029.375,"Apex.RT..min.":15.6,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (V4)","Search.ID":"V","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"APQALMLAPTR","Annotated.Sequence":"[K].APQALmLAPTR.[E]","Modifications":"M6(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7094,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":592.82658,"Contaminant":"False","Aligned.m.z..Da.":592.82663,"MH...Da.":1184.6459,"Theo..MH...Da.":1184.6456,"DeltaM..ppm.":0.25,"Deltam.z..Da.":0.00015,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1198519.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":26.99041,"Ion.Inject.Time..ms.":22,"RT..min.":10.1796,"First.Scan":8502,"Last.Scan":8502,"Master.Scan.s.":8494,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_03.raw","File.ID":"F15","Quan.Info":"","Peptides.Matched":304,"XCorr":2.65,"X..Protein.Groups":1,"Percolator.q.Value":0.00028,"Percolator.PEP":0.00001,"Percolator.SVMScore":1.747,"Precursor.Area":1717311.625,"Apex.RT..min.":10.2,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (V4)","Search.ID":"V","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DIFSNDELLSDAYDAK","Annotated.Sequence":"[K].DIFSNDELLSDAYDAK.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.6234,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":605.94761,"Contaminant":"False","Aligned.m.z..Da.":605.94751,"MH...Da.":1815.82828,"Theo..MH...Da.":1815.82831,"DeltaM..ppm.":-0.02,"Deltam.z..Da.":-1e-05,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":218172.15625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":17.0821,"First.Scan":17314,"Last.Scan":17314,"Master.Scan.s.":17303,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_03.raw","File.ID":"F15","Quan.Info":"","Peptides.Matched":1560,"XCorr":2.31,"X..Protein.Groups":1,"Percolator.q.Value":0.00028,"Percolator.PEP":0.00008,"Percolator.SVMScore":1.392,"Precursor.Area":393907.9375,"Apex.RT..min.":17.09,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (V4)","Search.ID":"V","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KDELTLEGIK","Annotated.Sequence":"[K].KDELTLEGIK.[Q]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5084,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":573.32439,"Contaminant":"False","Aligned.m.z..Da.":573.32443,"MH...Da.":1145.64151,"Theo..MH...Da.":1145.64123,"DeltaM..ppm.":0.24,"Deltam.z..Da.":0.00014,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3073305.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":4.16211,"Ion.Inject.Time..ms.":22,"RT..min.":10.2589,"First.Scan":8600,"Last.Scan":8600,"Master.Scan.s.":8598,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_03.raw","File.ID":"F15","Quan.Info":"","Peptides.Matched":307,"XCorr":3.56,"X..Protein.Groups":1,"Percolator.q.Value":0.00028,"Percolator.PEP":1.598e-08,"Percolator.SVMScore":2.948,"Precursor.Area":1846269.375,"Apex.RT..min.":10.26,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (V4)","Search.ID":"V","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DIFSNDELLSDAYDAK","Annotated.Sequence":"[K].DIFSNDELLSDAYDAK.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7437,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":908.41356,"Contaminant":"False","Aligned.m.z..Da.":908.41342,"MH...Da.":1815.81986,"Theo..MH...Da.":1815.82831,"DeltaM..ppm.":-4.66,"Deltam.z..Da.":-0.00423,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":319513.3125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":16.40364,"Ion.Inject.Time..ms.":22,"RT..min.":17.0662,"First.Scan":17295,"Last.Scan":17295,"Master.Scan.s.":17291,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_03.raw","File.ID":"F15","Quan.Info":"","Peptides.Matched":1558,"XCorr":3.94,"X..Protein.Groups":1,"Percolator.q.Value":0.00028,"Percolator.PEP":1.174e-08,"Percolator.SVMScore":3.004,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (V4)","Search.ID":"V","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].mSEGITDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7406,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1056.47922,"Contaminant":"False","Aligned.m.z..Da.":1056.47923,"MH...Da.":2111.95116,"Theo..MH...Da.":2111.96151,"DeltaM..ppm.":-4.9,"Deltam.z..Da.":-0.00518,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":237825.85938,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":92.55212,"Ion.Inject.Time..ms.":22,"RT..min.":14.6497,"First.Scan":14324,"Last.Scan":14324,"Master.Scan.s.":14312,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_03.raw","File.ID":"F15","Quan.Info":"","Peptides.Matched":2198,"XCorr":4.01,"X..Protein.Groups":1,"Percolator.q.Value":0.00028,"Percolator.PEP":2.626e-11,"Percolator.SVMScore":4.114,"Precursor.Area":1154318.625,"Apex.RT..min.":14.66,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (V4)","Search.ID":"V","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FTVSAIYSDLPQQER","Annotated.Sequence":"[K].FTVSAIYSDLPQQER.[D]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6858,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":877.44087,"Contaminant":"False","Aligned.m.z..Da.":877.44087,"MH...Da.":1753.87447,"Theo..MH...Da.":1753.87554,"DeltaM..ppm.":-0.61,"Deltam.z..Da.":-0.00053,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":438634.28125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":21.05324,"Ion.Inject.Time..ms.":22,"RT..min.":15.0119,"First.Scan":14749,"Last.Scan":14749,"Master.Scan.s.":14743,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_03.raw","File.ID":"F15","Quan.Info":"","Peptides.Matched":1160,"XCorr":3.66,"X..Protein.Groups":1,"Percolator.q.Value":0.00028,"Percolator.PEP":7.479e-10,"Percolator.SVMScore":3.505,"Precursor.Area":514837.03125,"Apex.RT..min.":15.02,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (V4)","Search.ID":"V","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"GVFGYGFEEPSAIQQR","Annotated.Sequence":"[R].GVFGYGFEEPSAIQQR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7032,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":892.93237,"Contaminant":"False","Aligned.m.z..Da.":892.93236,"MH...Da.":1784.85746,"Theo..MH...Da.":1784.86022,"DeltaM..ppm.":-1.55,"Deltam.z..Da.":-0.00138,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":513644.78125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":39.37102,"Ion.Inject.Time..ms.":22,"RT..min.":15.0113,"First.Scan":14748,"Last.Scan":14748,"Master.Scan.s.":14743,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_03.raw","File.ID":"F15","Quan.Info":"","Peptides.Matched":1410,"XCorr":3.74,"X..Protein.Groups":1,"Percolator.q.Value":0.00028,"Percolator.PEP":3.062e-09,"Percolator.SVMScore":3.248,"Precursor.Area":659225.75,"Apex.RT..min.":15.02,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (V4)","Search.ID":"V","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].mSEGITDIEEsQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S11(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.131,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1096.46424,"Contaminant":"False","Aligned.m.z..Da.":1096.46423,"MH...Da.":2191.9212,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":-3.03,"Deltam.z..Da.":-0.00332,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":357431.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":14.9944,"First.Scan":14728,"Last.Scan":14728,"Master.Scan.s.":14717,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_03.raw","File.ID":"F15","Quan.Info":"","Peptides.Matched":1893,"XCorr":3.13,"X..Protein.Groups":1,"Percolator.q.Value":0.00028,"Percolator.PEP":8.638e-07,"Percolator.SVMScore":2.223,"ptmRS..Binomial.Peptide.Score":306.73,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(14): 1665.76; y1+-H(3) O(4) P(12): 1449.69; y1+-H(3) O(4) P(11): 1336.60; y1+-H(3) O(4) P(10): 1207.56; y1+-H(3) O(4) P(9): 1078.52; b1+(8): 758.36","ptmRS..Best.Site.Probabilities":"S11(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; T(6): 0.0; S(11): 100.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (H4)","Search.ID":"H","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"TAVFYGGTPISK","Annotated.Sequence":"[K].TAVFYGGTPISK.[D]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5943,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":620.83145,"Contaminant":"False","Aligned.m.z..Da.":620.83135,"MH...Da.":1240.65562,"Theo..MH...Da.":1240.65721,"DeltaM..ppm.":-1.28,"Deltam.z..Da.":-0.0008,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":797418.9375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":56.38699,"Ion.Inject.Time..ms.":22,"RT..min.":11.2191,"First.Scan":10630,"Last.Scan":10630,"Master.Scan.s.":10619,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_05.raw","File.ID":"F29","Quan.Info":"","Peptides.Matched":397,"XCorr":2.44,"X..Protein.Groups":1,"Percolator.q.Value":0.00028,"Percolator.PEP":0.00049,"Percolator.SVMScore":1.255,"Precursor.Area":681135.875,"Apex.RT..min.":11.22,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (H4)","Search.ID":"H","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MGSINNNFNTNNNSNTDLDR","Annotated.Sequence":"[-].mGSINNNFNTNNNsNTDLDR.[D]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S14(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P39517","Master.Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","Protein.Accessions":"P39517","Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0565,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":749.31117,"Contaminant":"False","Aligned.m.z..Da.":749.31106,"MH...Da.":2245.91897,"Theo..MH...Da.":2245.91057,"DeltaM..ppm.":3.74,"Deltam.z..Da.":0.0028,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":728751.3125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":36.389,"Ion.Inject.Time..ms.":22,"RT..min.":11.9086,"First.Scan":11571,"Last.Scan":11571,"Master.Scan.s.":11568,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_05.raw","File.ID":"F29","Quan.Info":"","Peptides.Matched":1457,"XCorr":3.01,"X..Protein.Groups":1,"Percolator.q.Value":0.00028,"Percolator.PEP":0.00066,"Percolator.SVMScore":1.166,"Precursor.Area":451860.96875,"Apex.RT..min.":11.91,"ptmRS..Binomial.Peptide.Score":217.98,"ptmRS..Isoform.Confidence.Probability":0.991,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(11): 1245.55; y1+-H(3) O(4) P(10): 1144.50; y1+-H(3) O(4) P(9): 1030.45; y1+-H(3) O(4) P(8): 916.41; y1+-H(3) O(4) P(7): 802.37; y2+-H(3) O(4) P(7): 401.69; y1+(5): 619.30","ptmRS..Best.Site.Probabilities":"S14(Phospho): 99.13","ptmRS..Phospho.Site.Probabilities":"S(3): 0.0; T(10): 0.0; S(14): 99.1; T(16): 0.9"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (H4)","Search.ID":"H","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LQQTAFDKK","Annotated.Sequence":"[R].LQQTAFDKK.[S]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.3835,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":360.20098,"Contaminant":"False","Aligned.m.z..Da.":360.20099,"MH...Da.":1078.58841,"Theo..MH...Da.":1078.58914,"DeltaM..ppm.":-0.68,"Deltam.z..Da.":-0.00024,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2791070.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":50.30458,"Ion.Inject.Time..ms.":18.986,"RT..min.":5.561,"First.Scan":2662,"Last.Scan":2662,"Master.Scan.s.":2650,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_05.raw","File.ID":"F29","Quan.Info":"","Peptides.Matched":383,"XCorr":2.66,"X..Protein.Groups":1,"Percolator.q.Value":0.00028,"Percolator.PEP":0.00048,"Percolator.SVMScore":1.256,"Precursor.Area":3554037.25,"Apex.RT..min.":5.57,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (H4)","Search.ID":"H","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"VKSPLDDK","Annotated.Sequence":"[K].VKsPLDDK.[L]","Modifications":"S3(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5606,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":491.23658,"Contaminant":"False","Aligned.m.z..Da.":491.23657,"MH...Da.":981.46589,"Theo..MH...Da.":981.46525,"DeltaM..ppm.":0.65,"Deltam.z..Da.":0.00032,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2458660.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":37.13239,"Ion.Inject.Time..ms.":22,"RT..min.":5.0881,"First.Scan":2012,"Last.Scan":2012,"Master.Scan.s.":2000,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_05.raw","File.ID":"F29","Quan.Info":"","Peptides.Matched":496,"XCorr":1.98,"X..Protein.Groups":1,"Percolator.q.Value":0.00028,"Percolator.PEP":0.00111,"Percolator.SVMScore":1.025,"Precursor.Area":1790322.875,"Apex.RT..min.":5.08,"ptmRS..Binomial.Peptide.Score":239.78,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"S3(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(3): 100.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (H4)","Search.ID":"H","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FFISNPDLVDRLEK","Annotated.Sequence":"[R].FFISNPDLVDRLEK.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q03558","Master.Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","Protein.Accessions":"Q03558","Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.6471,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":564.96807,"Contaminant":"False","Aligned.m.z..Da.":564.96797,"MH...Da.":1692.88967,"Theo..MH...Da.":1692.89555,"DeltaM..ppm.":-3.47,"Deltam.z..Da.":-0.00196,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":668337.0625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":51.57784,"Ion.Inject.Time..ms.":22,"RT..min.":15.291,"First.Scan":15895,"Last.Scan":15895,"Master.Scan.s.":15890,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_05.raw","File.ID":"F29","Quan.Info":"","Peptides.Matched":633,"XCorr":1.7,"X..Protein.Groups":1,"Percolator.q.Value":0.00028,"Percolator.PEP":0.00058,"Percolator.SVMScore":1.203,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (H4)","Search.ID":"H","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"GVFGYGFEEPSAIQQR","Annotated.Sequence":"[R].GVFGYGFEEPSAIQQR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.4551,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":595.62474,"Contaminant":"False","Aligned.m.z..Da.":595.62469,"MH...Da.":1784.85968,"Theo..MH...Da.":1784.86022,"DeltaM..ppm.":-0.31,"Deltam.z..Da.":-0.00018,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":352270.21875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":32.29951,"Ion.Inject.Time..ms.":22,"RT..min.":15.0801,"First.Scan":15615,"Last.Scan":15615,"Master.Scan.s.":15605,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_05.raw","File.ID":"F29","Quan.Info":"","Peptides.Matched":1293,"XCorr":1.78,"X..Protein.Groups":1,"Percolator.q.Value":0.00028,"Percolator.PEP":0.00116,"Percolator.SVMScore":1.011,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (H4)","Search.ID":"H","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].msHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss); S2(Phospho); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.4272,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":980.05281,"Contaminant":"False","Aligned.m.z..Da.":980.05277,"MH...Da.":2938.14388,"Theo..MH...Da.":2938.15509,"DeltaM..ppm.":-3.82,"Deltam.z..Da.":-0.00374,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":461834.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":74.53309,"Ion.Inject.Time..ms.":22,"RT..min.":14.9446,"First.Scan":15437,"Last.Scan":15437,"Master.Scan.s.":15434,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_05.raw","File.ID":"F29","Quan.Info":"","Peptides.Matched":1087,"XCorr":2.06,"X..Protein.Groups":1,"Percolator.q.Value":0.00028,"Percolator.PEP":0.0009,"Percolator.SVMScore":1.078,"Precursor.Area":921074.375,"Apex.RT..min.":14.97,"ptmRS..Binomial.Peptide.Score":189.28,"ptmRS..Isoform.Confidence.Probability":0.5,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"S2(Phospho): 99.98; Y12(Phospho): 50.01; S13(Phospho): 50.01","ptmRS..Phospho.Site.Probabilities":"S(2): 100.0; Y(12): 50.0; S(13): 50.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (H4)","Search.ID":"H","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MFILDEADEMLSSGFK","Annotated.Sequence":"[K].mFILDEADEmLSSGFK.[E]","Modifications":"M1(Oxidation); M10(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.6514,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":622.28404,"Contaminant":"False","Aligned.m.z..Da.":622.28384,"MH...Da.":1864.83758,"Theo..MH...Da.":1864.83433,"DeltaM..ppm.":1.75,"Deltam.z..Da.":0.00108,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":129496.82031,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":16.7606,"First.Scan":17556,"Last.Scan":17556,"Master.Scan.s.":17554,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_05.raw","File.ID":"F29","Quan.Info":"","Peptides.Matched":1873,"XCorr":2.18,"X..Protein.Groups":1,"Percolator.q.Value":0.00028,"Percolator.PEP":0.00044,"Percolator.SVMScore":1.281,"Precursor.Area":367821.15625,"Apex.RT..min.":16.82,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (H4)","Search.ID":"H","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LEAGLSDSK","Annotated.Sequence":"[K].LEAGLsDSK.[Q]","Modifications":"S6(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":3,"DeltaScore":0.0702,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":500.22339,"Contaminant":"False","Aligned.m.z..Da.":500.22349,"MH...Da.":999.43951,"Theo..MH...Da.":999.43943,"DeltaM..ppm.":0.07,"Deltam.z..Da.":0.00004,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":4622327.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":50.96643,"Ion.Inject.Time..ms.":17.179,"RT..min.":7.6906,"First.Scan":5928,"Last.Scan":5928,"Master.Scan.s.":5926,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_05.raw","File.ID":"F29","Quan.Info":"","Peptides.Matched":608,"XCorr":2.42,"X..Protein.Groups":1,"Percolator.q.Value":0.00028,"Percolator.PEP":0.00106,"Percolator.SVMScore":1.035,"Precursor.Area":3070416.5,"Apex.RT..min.":7.69,"ptmRS..Binomial.Peptide.Score":225.56,"ptmRS..Isoform.Confidence.Probability":0.996,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(8): 788.38; y1+-H(3) O(4) P(7): 659.34; y1+-H(3) O(4) P(6): 588.30; y1+(3): 349.17; y1+(2): 234.14","ptmRS..Best.Site.Probabilities":"S6(Phospho): 99.65","ptmRS..Phospho.Site.Probabilities":"S(6): 99.7; S(8): 0.4"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AA4)","Search.ID":"AA","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ILISTDLLAR","Annotated.Sequence":"[R].ILISTDLLAR.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":557.84538,"Contaminant":"False","Aligned.m.z..Da.":557.8455,"MH...Da.":1114.68349,"Theo..MH...Da.":1114.68304,"DeltaM..ppm.":0.41,"Deltam.z..Da.":0.00023,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":568952.1875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":30.50175,"Ion.Inject.Time..ms.":22,"RT..min.":15.6246,"First.Scan":15439,"Last.Scan":15439,"Master.Scan.s.":15429,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_04.raw","File.ID":"F10","Quan.Info":"","Peptides.Matched":124,"XCorr":2.16,"X..Protein.Groups":1,"Percolator.q.Value":0.00029,"Percolator.PEP":0.00028,"Percolator.SVMScore":1.214,"Precursor.Area":582590.1875,"Apex.RT..min.":15.6,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AA4)","Search.ID":"AA","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].mSEGITDIEESQIQTnYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); N16(Deamidated)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.583,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1056.97633,"Contaminant":"False","Aligned.m.z..Da.":1056.97671,"MH...Da.":2112.94538,"Theo..MH...Da.":2112.94553,"DeltaM..ppm.":-0.07,"Deltam.z..Da.":-0.00008,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1598278.375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":77.86292,"Ion.Inject.Time..ms.":22,"RT..min.":14.6693,"First.Scan":14298,"Last.Scan":14298,"Master.Scan.s.":14288,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_04.raw","File.ID":"F10","Quan.Info":"","Peptides.Matched":2091,"XCorr":2.23,"X..Protein.Groups":1,"Percolator.q.Value":0.00029,"Percolator.PEP":0.0001,"Percolator.SVMScore":1.41,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AA4)","Search.ID":"AA","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSSPLHRLSPTGK","Annotated.Sequence":"[R].mSsPLHRLsPTGK.[S]","Modifications":"M1(Oxidation); S3(Phospho); S9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.4123,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":529.56519,"Contaminant":"False","Aligned.m.z..Da.":529.56521,"MH...Da.":1586.68102,"Theo..MH...Da.":1586.67977,"DeltaM..ppm.":0.79,"Deltam.z..Da.":0.00042,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1682737.625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":9.85832,"Ion.Inject.Time..ms.":22,"RT..min.":7.7285,"First.Scan":5609,"Last.Scan":5609,"Master.Scan.s.":5600,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_04.raw","File.ID":"F10","Quan.Info":"","Peptides.Matched":1071,"XCorr":3.25,"X..Protein.Groups":1,"Percolator.q.Value":0.00029,"Percolator.PEP":0.00012,"Percolator.SVMScore":1.38,"Precursor.Area":1745190.125,"Apex.RT..min.":7.73,"ptmRS..Binomial.Peptide.Score":259.25,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(9): 990.58; y1+-H(3) O(4) P(8): 877.50; y2+-H(3) O(4) P(12): 671.34; y2+-H(3) O(4) P(11): 627.82; y2+-H(3) O(4) P(10): 544.32; y2+-H(3) O(4) P(8): 439.25; y1+(4): 402.23; y1+(3): 305.18","ptmRS..Best.Site.Probabilities":"S3(Phospho): 100; S9(Phospho): 99.99","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(3): 100.0; S(9): 100.0; T(11): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AF4)","Search.ID":"AF","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ELALQIQK","Annotated.Sequence":"[R].ELALQIQK.[V]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.2746,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":471.78468,"Contaminant":"False","Aligned.m.z..Da.":471.78474,"MH...Da.":942.56209,"Theo..MH...Da.":942.56186,"DeltaM..ppm.":0.24,"Deltam.z..Da.":0.00011,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":486804,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":11.72944,"Ion.Inject.Time..ms.":22,"RT..min.":10.579,"First.Scan":9114,"Last.Scan":9114,"Master.Scan.s.":9103,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y25_05.raw","File.ID":"F5","Quan.Info":"","Peptides.Matched":292,"XCorr":1.93,"X..Protein.Groups":1,"Percolator.q.Value":0.00029,"Percolator.PEP":0.00381,"Percolator.SVMScore":0.814,"Precursor.Area":407516,"Apex.RT..min.":10.58,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (S4)","Search.ID":"S","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LEAGLSDSK","Annotated.Sequence":"[K].LEAGLsDSK.[Q]","Modifications":"S6(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.088,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":500.22377,"Contaminant":"False","Aligned.m.z..Da.":500.22382,"MH...Da.":999.44027,"Theo..MH...Da.":999.43943,"DeltaM..ppm.":0.84,"Deltam.z..Da.":0.00042,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1747187.375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":27.00287,"Ion.Inject.Time..ms.":22,"RT..min.":7.707,"First.Scan":5809,"Last.Scan":5809,"Master.Scan.s.":5800,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_06.raw","File.ID":"F18","Quan.Info":"","Peptides.Matched":721,"XCorr":2.16,"X..Protein.Groups":1,"Percolator.q.Value":0.0003,"Percolator.PEP":0.00336,"Percolator.SVMScore":0.767,"Precursor.Area":1339170.625,"Apex.RT..min.":7.71,"ptmRS..Binomial.Peptide.Score":238.53,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(8): 788.38; y1+-H(3) O(4) P(7): 659.34; y1+-H(3) O(4) P(6): 588.30; y1+-H(3) O(4) P(5): 531.28; y1+-H(3) O(4) P(4): 418.19; y2+-H(3) O(4) P(6): 294.65; y1+(3): 349.17; y1+(2): 234.14","ptmRS..Best.Site.Probabilities":"S6(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(6): 100.0; S(8): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (S4)","Search.ID":"S","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"IQDPQMTGYVSTR","Annotated.Sequence":"[R].IQDPQmtGYVsTR.[Y]","Modifications":"M6(Oxidation); T7(Phospho); S11(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P32485","Master.Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","Protein.Accessions":"P32485","Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.064,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":836.32774,"Contaminant":"False","Aligned.m.z..Da.":836.32782,"MH...Da.":1671.64822,"Theo..MH...Da.":1671.64853,"DeltaM..ppm.":-0.19,"Deltam.z..Da.":-0.00016,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1699824.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":46.79555,"Ion.Inject.Time..ms.":22,"RT..min.":7.7151,"First.Scan":5819,"Last.Scan":5819,"Master.Scan.s.":5813,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_06.raw","File.ID":"F18","Quan.Info":"","Peptides.Matched":764,"XCorr":2.03,"X..Protein.Groups":1,"Percolator.q.Value":0.0003,"Percolator.PEP":0.00351,"Percolator.SVMScore":0.758,"Precursor.Area":1215691.625,"Apex.RT..min.":7.71,"ptmRS..Binomial.Peptide.Score":225.23,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(11): 1332.53; y1+-H(3) O(4) P(10): 1217.50; y1+-H(3) O(4) P(8): 992.39; y1+-H(3) O(4) P(7): 845.36; y2+-H(3) O(4) P(10): 609.25; y1+(4): 462.27; y1+(3): 363.20","ptmRS..Best.Site.Probabilities":"T7(Phospho): 100; Y9(Phospho): 99.99","ptmRS..Phospho.Site.Probabilities":"T(7): 100.0; Y(9): 100.0; S(11): 0.0; T(12): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (S4)","Search.ID":"S","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DFLLKPELSR","Annotated.Sequence":"[K].DFLLKPELSR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.6111,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":406.56721,"Contaminant":"False","Aligned.m.z..Da.":406.56718,"MH...Da.":1217.68708,"Theo..MH...Da.":1217.68885,"DeltaM..ppm.":-1.45,"Deltam.z..Da.":-0.00059,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":314617.90625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":32.28327,"Ion.Inject.Time..ms.":22,"RT..min.":13.1824,"First.Scan":13155,"Last.Scan":13155,"Master.Scan.s.":13154,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_06.raw","File.ID":"F18","Quan.Info":"","Peptides.Matched":283,"XCorr":1.62,"X..Protein.Groups":1,"Percolator.q.Value":0.0003,"Percolator.PEP":0.00171,"Percolator.SVMScore":0.902,"Precursor.Area":231144.42188,"Apex.RT..min.":13.17,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (S4)","Search.ID":"S","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KSDEPSRESTPVR","Annotated.Sequence":"[K].KSDEPsREsTPVR.[S]","Modifications":"S6(Phospho); S9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q08421","Master.Protein.Descriptions":"Enhancer of translation termination 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ETT1 PE=1 SV=1","Protein.Accessions":"Q08421","Protein.Descriptions":"Enhancer of translation termination 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ETT1 PE=1 SV=1","X..Missed.Cleavages":2,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.166,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":824.34234,"Contaminant":"False","Aligned.m.z..Da.":824.34223,"MH...Da.":1647.67741,"Theo..MH...Da.":1647.67752,"DeltaM..ppm.":-0.07,"Deltam.z..Da.":-0.00006,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":8781620,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":39.83472,"Ion.Inject.Time..ms.":16.424,"RT..min.":4.5053,"First.Scan":1403,"Last.Scan":1403,"Master.Scan.s.":1393,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_06.raw","File.ID":"F18","Quan.Info":"","Peptides.Matched":966,"XCorr":2.35,"X..Protein.Groups":1,"Percolator.q.Value":0.0003,"Percolator.PEP":0.00447,"Percolator.SVMScore":0.709,"Precursor.Area":4421046.5,"Apex.RT..min.":4.5,"ptmRS..Binomial.Peptide.Score":363.8,"ptmRS..Isoform.Confidence.Probability":0.993,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(10): 1219.55; y1+-H(3) O(4) P(9): 1090.50; y2+-H(3) O(4) P(10): 610.28; y2+-H(3) O(4) P(9): 545.76; b1+- -2H(3) O(4) P -2H(3) O(4) P(9): 980.44; b1+- -2H(3) O(4) P -2H(3) O(4) P(10): 1081.49","ptmRS..Best.Site.Probabilities":"S6(Phospho): 100; S9(Phospho): 99.32","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(6): 100.0; S(9): 99.3; T(10): 0.7"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (N4)","Search.ID":"N","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].msHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss); S2(Phospho); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.5543,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":980.0521,"Contaminant":"False","Aligned.m.z..Da.":980.05209,"MH...Da.":2938.14177,"Theo..MH...Da.":2938.15509,"DeltaM..ppm.":-4.53,"Deltam.z..Da.":-0.00444,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":249512.20312,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":24.62004,"Ion.Inject.Time..ms.":22,"RT..min.":14.9464,"First.Scan":15157,"Last.Scan":15157,"Master.Scan.s.":15153,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_05.raw","File.ID":"F23","Quan.Info":"","Peptides.Matched":951,"XCorr":2.58,"X..Protein.Groups":1,"Percolator.q.Value":0.0003,"Percolator.PEP":0.00185,"Percolator.SVMScore":0.906,"Precursor.Area":352751.59375,"Apex.RT..min.":14.96,"ptmRS..Binomial.Peptide.Score":177.64,"ptmRS..Isoform.Confidence.Probability":0.495,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"S2(Phospho): 99.09; Y12(Phospho): 50.46; S13(Phospho): 50.46","ptmRS..Phospho.Site.Probabilities":"S(2): 99.1; Y(12): 50.5; S(13): 50.5; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AJ4)","Search.ID":"AJ","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"IQDPQMTGYVSTR","Annotated.Sequence":"[R].IQDPQMTGyVSTR.[Y]","Modifications":"Y9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P32485","Master.Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","Protein.Accessions":"P32485","Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.0973,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":788.34434,"Contaminant":"False","Aligned.m.z..Da.":788.34439,"MH...Da.":1575.68141,"Theo..MH...Da.":1575.68728,"DeltaM..ppm.":-3.73,"Deltam.z..Da.":-0.00294,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":408092,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":49.63621,"Ion.Inject.Time..ms.":22,"RT..min.":9.5914,"First.Scan":7922,"Last.Scan":7922,"Master.Scan.s.":7914,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y25_01.raw","File.ID":"F1","Quan.Info":"","Peptides.Matched":1122,"XCorr":1.85,"X..Protein.Groups":1,"Percolator.q.Value":0.0003,"Percolator.PEP":0.00021,"Percolator.SVMScore":1.164,"Precursor.Area":251539.21875,"Apex.RT..min.":9.58,"ptmRS..Binomial.Peptide.Score":240.16,"ptmRS..Isoform.Confidence.Probability":0.989,"ptmRS..Isoform.Group.Report":"y1+(6): 762.32","ptmRS..Best.Site.Probabilities":"Y9(Phospho): 98.87","ptmRS..Phospho.Site.Probabilities":"T(7): 0.6; Y(9): 98.9; S(11): 0.0; T(12): 0.6"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AJ4)","Search.ID":"AJ","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DIFSNDELLSDAYDAK","Annotated.Sequence":"[K].DIFSNDELLSDAYDAK.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.8012,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":908.4182,"Contaminant":"False","Aligned.m.z..Da.":908.41812,"MH...Da.":1815.82912,"Theo..MH...Da.":1815.82831,"DeltaM..ppm.":0.44,"Deltam.z..Da.":0.0004,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":247229.10938,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":8.60356,"Ion.Inject.Time..ms.":22,"RT..min.":17.0846,"First.Scan":13972,"Last.Scan":13972,"Master.Scan.s.":13971,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y25_01.raw","File.ID":"F1","Quan.Info":"","Peptides.Matched":1570,"XCorr":3.37,"X..Protein.Groups":1,"Percolator.q.Value":0.0003,"Percolator.PEP":6.085e-09,"Percolator.SVMScore":3.208,"Precursor.Area":240489.42188,"Apex.RT..min.":17.09,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AJ4)","Search.ID":"AJ","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"GVFGYGFEEPSAIQQR","Annotated.Sequence":"[R].GVFGYGFEEPSAIQQR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6759,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":892.93262,"Contaminant":"False","Aligned.m.z..Da.":892.9327,"MH...Da.":1784.85796,"Theo..MH...Da.":1784.86022,"DeltaM..ppm.":-1.27,"Deltam.z..Da.":-0.00113,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":209930.96875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":17.88199,"Ion.Inject.Time..ms.":22,"RT..min.":15.0379,"First.Scan":12596,"Last.Scan":12596,"Master.Scan.s.":12591,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y25_01.raw","File.ID":"F1","Quan.Info":"","Peptides.Matched":1375,"XCorr":2.53,"X..Protein.Groups":1,"Percolator.q.Value":0.0003,"Percolator.PEP":7.739e-07,"Percolator.SVMScore":2.263,"Precursor.Area":144523.03125,"Apex.RT..min.":15.04,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AJ4)","Search.ID":"AJ","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"IQDPQMTGYVSTR","Annotated.Sequence":"[R].IQDPQmTGyVSTR.[Y]","Modifications":"M6(Oxidation); Y9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P32485","Master.Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","Protein.Accessions":"P32485","Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1987,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":796.34509,"Contaminant":"False","Aligned.m.z..Da.":796.34526,"MH...Da.":1591.6829,"Theo..MH...Da.":1591.6822,"DeltaM..ppm.":0.44,"Deltam.z..Da.":0.00035,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":373161.84375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":7.6719,"First.Scan":5166,"Last.Scan":5166,"Master.Scan.s.":5155,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y25_01.raw","File.ID":"F1","Quan.Info":"","Peptides.Matched":1255,"XCorr":1.51,"X..Protein.Groups":1,"Percolator.q.Value":0.0003,"Percolator.PEP":0.00013,"Percolator.SVMScore":1.268,"Precursor.Area":388303.34375,"Apex.RT..min.":7.67,"ptmRS..Binomial.Peptide.Score":216.05,"ptmRS..Isoform.Confidence.Probability":0.991,"ptmRS..Isoform.Group.Report":"y1+(6): 762.32","ptmRS..Best.Site.Probabilities":"Y9(Phospho): 99.1","ptmRS..Phospho.Site.Probabilities":"T(7): 0.5; Y(9): 99.1; S(11): 0.0; T(12): 0.5"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AJ4)","Search.ID":"AJ","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LQETNPEEVPK","Annotated.Sequence":"[K].LQETNPEEVPK.[F]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.4836,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":642.32744,"Contaminant":"False","Aligned.m.z..Da.":642.32757,"MH...Da.":1283.64761,"Theo..MH...Da.":1283.64777,"DeltaM..ppm.":-0.13,"Deltam.z..Da.":-0.00008,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":581761.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":23.86811,"Ion.Inject.Time..ms.":22,"RT..min.":7.5986,"First.Scan":5047,"Last.Scan":5047,"Master.Scan.s.":5042,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y25_01.raw","File.ID":"F1","Quan.Info":"","Peptides.Matched":551,"XCorr":2.13,"X..Protein.Groups":1,"Percolator.q.Value":0.0003,"Percolator.PEP":0.00008,"Percolator.SVMScore":1.361,"Precursor.Area":639265.875,"Apex.RT..min.":7.61,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AJ4)","Search.ID":"AJ","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"HSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[R].HSDSySENETNHTNVPISSTGGTNNK.[T]","Modifications":"Y5(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0276,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":957.40194,"Contaminant":"False","Aligned.m.z..Da.":957.40212,"MH...Da.":2870.19126,"Theo..MH...Da.":2870.18608,"DeltaM..ppm.":1.81,"Deltam.z..Da.":0.00173,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":184047.95312,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":7.5743,"First.Scan":5008,"Last.Scan":5008,"Master.Scan.s.":5003,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y25_01.raw","File.ID":"F1","Quan.Info":"","Peptides.Matched":2554,"XCorr":3.99,"X..Protein.Groups":1,"Percolator.q.Value":0.0003,"Percolator.PEP":4.034e-08,"Percolator.SVMScore":2.839,"Precursor.Area":559291.6875,"Apex.RT..min.":7.59,"ptmRS..Binomial.Peptide.Score":267.24,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(5): 572.21; b1+-H(3) O(4) P(6): 659.24; b1+-H(3) O(4) P(11): 1246.46; b2+-H(3) O(4) P(12): 692.26; b2+-H(3) O(4) P(13): 742.79; b2+-H(3) O(4) P(14): 799.81; b2+-H(3) O(4) P(15): 849.34; y2+-H(3) O(4) P(25): 1318.08; y2+-H(3) O(4) P(24): 1274.56","ptmRS..Best.Site.Probabilities":"S4(Phospho): 50; S6(Phospho): 50","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(4): 50.0; Y(5): 0.0; S(6): 50.0; T(10): 0.0; T(13): 0.0; S(18): 0.0; S(19): 0.0; T(20): 0.0; T(23): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AH4)","Search.ID":"AH","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ELALQIQK","Annotated.Sequence":"[R].ELALQIQK.[V]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.2291,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":471.7847,"Contaminant":"False","Aligned.m.z..Da.":471.78474,"MH...Da.":942.56213,"Theo..MH...Da.":942.56186,"DeltaM..ppm.":0.28,"Deltam.z..Da.":0.00013,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":455939.65625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":10.85138,"Ion.Inject.Time..ms.":22,"RT..min.":10.5958,"First.Scan":9139,"Last.Scan":9139,"Master.Scan.s.":9127,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y25_03.raw","File.ID":"F3","Quan.Info":"","Peptides.Matched":292,"XCorr":1.79,"X..Protein.Groups":1,"Percolator.q.Value":0.00031,"Percolator.PEP":0.00146,"Percolator.SVMScore":0.934,"Precursor.Area":346545.0625,"Apex.RT..min.":10.59,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (I4)","Search.ID":"I","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"APQALMLAPTR","Annotated.Sequence":"[K].APQALmLAPTR.[E]","Modifications":"M6(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7213,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":592.82657,"Contaminant":"False","Aligned.m.z..Da.":592.82652,"MH...Da.":1184.64587,"Theo..MH...Da.":1184.6456,"DeltaM..ppm.":0.22,"Deltam.z..Da.":0.00013,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2074994.625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":12.73422,"Ion.Inject.Time..ms.":22,"RT..min.":10.165,"First.Scan":8543,"Last.Scan":8543,"Master.Scan.s.":8537,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_04.raw","File.ID":"F28","Quan.Info":"","Peptides.Matched":304,"XCorr":3.05,"X..Protein.Groups":1,"Percolator.q.Value":0.00031,"Percolator.PEP":0.00022,"Percolator.SVMScore":1.767,"Precursor.Area":3925899.5,"Apex.RT..min.":10.19,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (I4)","Search.ID":"I","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DFLLKPELSR","Annotated.Sequence":"[K].DFLLKPELSR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.7012,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":406.56809,"Contaminant":"False","Aligned.m.z..Da.":406.56808,"MH...Da.":1217.68971,"Theo..MH...Da.":1217.68885,"DeltaM..ppm.":0.71,"Deltam.z..Da.":0.00029,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":692770.625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":3.61995,"Ion.Inject.Time..ms.":22,"RT..min.":13.1527,"First.Scan":12533,"Last.Scan":12533,"Master.Scan.s.":12528,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_04.raw","File.ID":"F28","Quan.Info":"","Peptides.Matched":265,"XCorr":2.51,"X..Protein.Groups":1,"Percolator.q.Value":0.00031,"Percolator.PEP":0.00014,"Percolator.SVMScore":1.946,"Precursor.Area":643738.75,"Apex.RT..min.":13.16,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (I4)","Search.ID":"I","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"TAVFYGGTPISK","Annotated.Sequence":"[K].TAVFYGGTPISK.[D]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5492,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":620.83222,"Contaminant":"False","Aligned.m.z..Da.":620.83219,"MH...Da.":1240.65717,"Theo..MH...Da.":1240.65721,"DeltaM..ppm.":-0.04,"Deltam.z..Da.":-0.00002,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1261544.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":59.27612,"Ion.Inject.Time..ms.":22,"RT..min.":11.1435,"First.Scan":9823,"Last.Scan":9823,"Master.Scan.s.":9811,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_04.raw","File.ID":"F28","Quan.Info":"","Peptides.Matched":385,"XCorr":2.44,"X..Protein.Groups":1,"Percolator.q.Value":0.00031,"Percolator.PEP":0.00061,"Percolator.SVMScore":1.35,"Precursor.Area":767543.75,"Apex.RT..min.":11.14,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (I4)","Search.ID":"I","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"SSQIHIENESTEDILK","Annotated.Sequence":"[R].SSQIHIENEStEDILK.[I]","Modifications":"T11(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.5117,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":641.62753,"Contaminant":"False","Aligned.m.z..Da.":641.62751,"MH...Da.":1922.86806,"Theo..MH...Da.":1922.87429,"DeltaM..ppm.":-3.24,"Deltam.z..Da.":-0.00208,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1772512,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":11.3141,"First.Scan":10055,"Last.Scan":10055,"Master.Scan.s.":10045,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_04.raw","File.ID":"F28","Quan.Info":"","Peptides.Matched":1740,"XCorr":2.99,"X..Protein.Groups":1,"Percolator.q.Value":0.00031,"Percolator.PEP":0.00022,"Percolator.SVMScore":1.765,"Precursor.Area":575299.4375,"Apex.RT..min.":11.41,"ptmRS..Binomial.Peptide.Score":306.83,"ptmRS..Isoform.Confidence.Probability":0.994,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(7): 787.42; y1+-H(3) O(4) P(6): 700.39; y2+-H(3) O(4) P(7): 394.21","ptmRS..Best.Site.Probabilities":"T11(Phospho): 99.4","ptmRS..Phospho.Site.Probabilities":"S(1): 0.0; S(2): 0.0; S(10): 0.6; T(11): 99.4"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (I4)","Search.ID":"I","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MFILDEADEMLSSGFK","Annotated.Sequence":"[K].mFILDEADEmLSSGFK.[E]","Modifications":"M1(Oxidation); M10(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.6284,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":622.28288,"Contaminant":"False","Aligned.m.z..Da.":622.28282,"MH...Da.":1864.8341,"Theo..MH...Da.":1864.83433,"DeltaM..ppm.":-0.12,"Deltam.z..Da.":-0.00008,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":685163.6875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":10.24022,"Ion.Inject.Time..ms.":22,"RT..min.":16.7049,"First.Scan":17032,"Last.Scan":17032,"Master.Scan.s.":17026,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_04.raw","File.ID":"F28","Quan.Info":"","Peptides.Matched":1861,"XCorr":2.61,"X..Protein.Groups":1,"Percolator.q.Value":0.00031,"Percolator.PEP":0.00055,"Percolator.SVMScore":1.39,"Precursor.Area":508310.625,"Apex.RT..min.":16.79,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (I4)","Search.ID":"I","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FDDMELDENLLR","Annotated.Sequence":"[K].FDDmELDENLLR.[G]","Modifications":"M4(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5469,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":763.34683,"Contaminant":"False","Aligned.m.z..Da.":763.34684,"MH...Da.":1525.68639,"Theo..MH...Da.":1525.6839,"DeltaM..ppm.":1.64,"Deltam.z..Da.":0.00125,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1112097.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":82.99718,"Ion.Inject.Time..ms.":22,"RT..min.":14.4692,"First.Scan":14211,"Last.Scan":14211,"Master.Scan.s.":14205,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_04.raw","File.ID":"F28","Quan.Info":"","Peptides.Matched":1033,"XCorr":2.56,"X..Protein.Groups":1,"Percolator.q.Value":0.00031,"Percolator.PEP":0.00019,"Percolator.SVMScore":1.846,"Precursor.Area":1519419.375,"Apex.RT..min.":14.49,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (I4)","Search.ID":"I","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LQQTAFDKK","Annotated.Sequence":"[R].LQQTAFDKK.[S]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.4697,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":360.20149,"Contaminant":"False","Aligned.m.z..Da.":360.20151,"MH...Da.":1078.58993,"Theo..MH...Da.":1078.58914,"DeltaM..ppm.":0.73,"Deltam.z..Da.":0.00026,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":5450713.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":15.39115,"Ion.Inject.Time..ms.":16.802,"RT..min.":5.4911,"First.Scan":2236,"Last.Scan":2236,"Master.Scan.s.":2232,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_04.raw","File.ID":"F28","Quan.Info":"","Peptides.Matched":392,"XCorr":2.64,"X..Protein.Groups":1,"Percolator.q.Value":0.00031,"Percolator.PEP":0.00051,"Percolator.SVMScore":1.425,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (I4)","Search.ID":"I","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KSDEPSRESTPVR","Annotated.Sequence":"[K].KSDEPsREsTPVR.[S]","Modifications":"S6(Phospho); S9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q08421","Master.Protein.Descriptions":"Enhancer of translation termination 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ETT1 PE=1 SV=1","Protein.Accessions":"Q08421","Protein.Descriptions":"Enhancer of translation termination 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ETT1 PE=1 SV=1","X..Missed.Cleavages":2,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1404,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":824.34219,"Contaminant":"False","Aligned.m.z..Da.":824.3421,"MH...Da.":1647.67712,"Theo..MH...Da.":1647.67752,"DeltaM..ppm.":-0.25,"Deltam.z..Da.":-0.0002,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":9761808,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":4.5627,"First.Scan":1323,"Last.Scan":1323,"Master.Scan.s.":1322,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_04.raw","File.ID":"F28","Quan.Info":"","Peptides.Matched":973,"XCorr":2.92,"X..Protein.Groups":1,"Percolator.q.Value":0.00031,"Percolator.PEP":0.00017,"Percolator.SVMScore":1.879,"Precursor.Area":6988928.5,"Apex.RT..min.":4.55,"ptmRS..Binomial.Peptide.Score":369.65,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(9): 998.45; y1+-H(3) O(4) P(10): 1219.55; y1+-H(3) O(4) P(9): 1090.50; y1+-H(3) O(4) P(4): 454.28; y2+-H(3) O(4) P(10): 610.28; y2+-H(3) O(4) P(9): 545.76; b1+- -2H(3) O(4) P -2H(3) O(4) P(10): 1081.49; b1+- -2H(3) O(4) P -2H(3) O(4) P(12): 1277.61","ptmRS..Best.Site.Probabilities":"S6(Phospho): 100; T10(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(6): 100.0; S(9): 0.0; T(10): 100.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (I4)","Search.ID":"I","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"IGNNELLHR","Annotated.Sequence":"[K].IGNNELLHR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q03558","Master.Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","Protein.Accessions":"Q03558","Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.5898,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":355.86487,"Contaminant":"False","Aligned.m.z..Da.":355.86489,"MH...Da.":1065.58007,"Theo..MH...Da.":1065.57997,"DeltaM..ppm.":0.09,"Deltam.z..Da.":0.00003,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1899644.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":17.16881,"Ion.Inject.Time..ms.":22,"RT..min.":7.5831,"First.Scan":5403,"Last.Scan":5403,"Master.Scan.s.":5391,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_04.raw","File.ID":"F28","Quan.Info":"","Peptides.Matched":300,"XCorr":2.56,"X..Protein.Groups":1,"Percolator.q.Value":0.00031,"Percolator.PEP":0.0004,"Percolator.SVMScore":1.527,"Precursor.Area":1872468.125,"Apex.RT..min.":7.58,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (I4)","Search.ID":"I","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"IQDPQMTGYVSTR","Annotated.Sequence":"[R].IQDPQmtGYVsTR.[Y]","Modifications":"M6(Oxidation); T7(Phospho); S11(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P32485","Master.Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","Protein.Accessions":"P32485","Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.0345,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":836.32831,"Contaminant":"False","Aligned.m.z..Da.":836.32838,"MH...Da.":1671.64935,"Theo..MH...Da.":1671.64853,"DeltaM..ppm.":0.49,"Deltam.z..Da.":0.00041,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3337032,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":4.69742,"Ion.Inject.Time..ms.":22,"RT..min.":7.7532,"First.Scan":5637,"Last.Scan":5637,"Master.Scan.s.":5625,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_04.raw","File.ID":"F28","Quan.Info":"","Peptides.Matched":756,"XCorr":3.19,"X..Protein.Groups":1,"Percolator.q.Value":0.00031,"Percolator.PEP":0.00025,"Percolator.SVMScore":1.715,"Precursor.Area":3168291.75,"Apex.RT..min.":7.76,"ptmRS..Binomial.Peptide.Score":338.57,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(11): 1332.53; y1+-H(3) O(4) P(10): 1217.50; y1+-H(3) O(4) P(8): 992.39; y1+-H(3) O(4) P(7): 845.36; y2+-H(3) O(4) P(11): 666.77; y2+-H(3) O(4) P(10): 609.25; y1+(4): 462.27; y1+(3): 363.20","ptmRS..Best.Site.Probabilities":"T7(Phospho): 100; Y9(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"T(7): 100.0; Y(9): 100.0; S(11): 0.0; T(12): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (I4)","Search.ID":"I","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSSPLHRLSPTGK","Annotated.Sequence":"[R].mSsPLHRLsPTGK.[S]","Modifications":"M1(Oxidation); S3(Phospho); S9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.3993,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":793.84333,"Contaminant":"False","Aligned.m.z..Da.":793.84339,"MH...Da.":1586.67939,"Theo..MH...Da.":1586.67977,"DeltaM..ppm.":-0.24,"Deltam.z..Da.":-0.00019,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3329151,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":18.4016,"Ion.Inject.Time..ms.":22,"RT..min.":7.7415,"First.Scan":5621,"Last.Scan":5621,"Master.Scan.s.":5612,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_04.raw","File.ID":"F28","Quan.Info":"","Peptides.Matched":1026,"XCorr":2.93,"X..Protein.Groups":1,"Percolator.q.Value":0.00031,"Percolator.PEP":0.00031,"Percolator.SVMScore":1.626,"Precursor.Area":2750690.25,"Apex.RT..min.":7.74,"ptmRS..Binomial.Peptide.Score":310.93,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(3): 304.10; b1+-H(3) O(4) P(8): 920.48; y2+-H(3) O(4) P(11): 627.82; y2+- -2H(3) O(4) P -2H(3) O(4) P(11): 578.83; b1+(2): 235.07","ptmRS..Best.Site.Probabilities":"S3(Phospho): 100; S9(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(3): 100.0; S(9): 100.0; T(11): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (I4)","Search.ID":"I","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSSPLHRLSPTGK","Annotated.Sequence":"[R].mSsPLHRLsPTGK.[S]","Modifications":"M1(Oxidation); S3(Phospho); S9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.4527,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":529.56463,"Contaminant":"False","Aligned.m.z..Da.":529.56466,"MH...Da.":1586.67934,"Theo..MH...Da.":1586.67977,"DeltaM..ppm.":-0.27,"Deltam.z..Da.":-0.00015,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":4926483.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":7.7174,"First.Scan":5587,"Last.Scan":5587,"Master.Scan.s.":5586,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_04.raw","File.ID":"F28","Quan.Info":"","Peptides.Matched":1007,"XCorr":4.02,"X..Protein.Groups":1,"Percolator.q.Value":0.00031,"Percolator.PEP":0.00042,"Percolator.SVMScore":1.51,"Precursor.Area":6885107.5,"Apex.RT..min.":7.74,"ptmRS..Binomial.Peptide.Score":259.25,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(8): 877.50; y1+-H(3) O(4) P(7): 740.44; y2+-H(3) O(4) P(11): 627.82; y2+-H(3) O(4) P(10): 544.32; y2+-H(3) O(4) P(8): 439.25; y1+(4): 402.23; y1+(3): 305.18","ptmRS..Best.Site.Probabilities":"S3(Phospho): 100; S9(Phospho): 99.99","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(3): 100.0; S(9): 100.0; T(11): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (L4)","Search.ID":"L","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"AAEAGETGAATSATEGDNNNNTAAGDKK","Annotated.Sequence":"[K].AAEAGETGAATSATEGDNNNNTAAGDKK.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.7346,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":879.39355,"Contaminant":"False","Aligned.m.z..Da.":879.39353,"MH...Da.":2636.16609,"Theo..MH...Da.":2636.16665,"DeltaM..ppm.":-0.21,"Deltam.z..Da.":-0.00019,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":4006443.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":2.94011,"Ion.Inject.Time..ms.":22,"RT..min.":4.9355,"First.Scan":1697,"Last.Scan":1697,"Master.Scan.s.":1686,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_01.raw","File.ID":"F25","Quan.Info":"","Peptides.Matched":3597,"XCorr":6.33,"X..Protein.Groups":1,"Percolator.q.Value":0.00033,"Percolator.PEP":9.07512e-17,"Percolator.SVMScore":9.67925,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (L4)","Search.ID":"L","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"AIMPIIEGHDVLAQAQSGTGK","Annotated.Sequence":"[R].AImPIIEGHDVLAQAQSGTGK.[T]","Modifications":"M3(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.7483,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":718.04023,"Contaminant":"False","Aligned.m.z..Da.":718.0403,"MH...Da.":2152.10614,"Theo..MH...Da.":2152.10668,"DeltaM..ppm.":-0.25,"Deltam.z..Da.":-0.00018,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1146196.125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":8.60981,"Ion.Inject.Time..ms.":22,"RT..min.":12.746,"First.Scan":11831,"Last.Scan":11831,"Master.Scan.s.":11826,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_01.raw","File.ID":"F25","Quan.Info":"","Peptides.Matched":1010,"XCorr":4.45,"X..Protein.Groups":1,"Percolator.q.Value":0.00033,"Percolator.PEP":8.242e-11,"Percolator.SVMScore":4.302,"Precursor.Area":1311025.5,"Apex.RT..min.":12.76,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (L4)","Search.ID":"L","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"MGSINNNFNTNNNSNTDLDRDWK","Annotated.Sequence":"[-].mGSINNNFNTNNNsNTDLDRDWK.[T]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S14(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P39517","Master.Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","Protein.Accessions":"P39517","Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.0409,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1338.06227,"Contaminant":"False","Aligned.m.z..Da.":1338.06262,"MH...Da.":2675.11727,"Theo..MH...Da.":2675.11179,"DeltaM..ppm.":2.05,"Deltam.z..Da.":0.00274,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":465055.625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":13.5577,"First.Scan":12888,"Last.Scan":12888,"Master.Scan.s.":12879,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_01.raw","File.ID":"F25","Quan.Info":"","Peptides.Matched":2380,"XCorr":5.14,"X..Protein.Groups":1,"Percolator.q.Value":0.00033,"Percolator.PEP":1.139e-11,"Percolator.SVMScore":4.688,"Precursor.Area":789926.5,"Apex.RT..min.":13.57,"ptmRS..Binomial.Peptide.Score":622.69,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(20): 2129.92; y1+-H(3) O(4) P(15): 1788.79; y1+-H(3) O(4) P(14): 1674.75; y1+-H(3) O(4) P(13): 1573.70; y1+-H(3) O(4) P(12): 1459.66; y1+-H(3) O(4) P(10): 1231.57; b2+-H(3) O(4) P(19): 987.41; b2+-H(3) O(4) P(21): 1122.98; y2+-H(3) O(4) P(17): 1025.45; y2+-H(3) O(4) P(15): 894.90; y1+(9): 1162.55; y1+(8): 1048.51","ptmRS..Best.Site.Probabilities":"S14(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(3): 0.0; T(10): 0.0; S(14): 100.0; T(16): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (L4)","Search.ID":"L","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"MGSINNNFNTNNNSNTDLDRDWK","Annotated.Sequence":"[-].mGSINNNFNTNNNsNTDLDRDWK.[T]","Modifications":"N-Term(Prot)(Met-loss); S14(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P39517","Master.Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","Protein.Accessions":"P39517","Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0284,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":878.37164,"Contaminant":"False","Aligned.m.z..Da.":878.37162,"MH...Da.":2633.10036,"Theo..MH...Da.":2633.10123,"DeltaM..ppm.":-0.33,"Deltam.z..Da.":-0.00029,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":736581.875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":11.2741,"First.Scan":9802,"Last.Scan":9802,"Master.Scan.s.":9798,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_01.raw","File.ID":"F25","Quan.Info":"","Peptides.Matched":2173,"XCorr":5.28,"X..Protein.Groups":1,"Percolator.q.Value":0.00033,"Percolator.PEP":2.071e-13,"Percolator.SVMScore":5.471,"Precursor.Area":5246860,"Apex.RT..min.":11.3,"ptmRS..Binomial.Peptide.Score":541.14,"ptmRS..Isoform.Confidence.Probability":0.994,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(12): 1459.66; y1+-H(3) O(4) P(11): 1345.61; y1+-H(3) O(4) P(10): 1231.57; y2+-H(3) O(4) P(17): 1025.45; y2+-H(3) O(4) P(16): 968.43; y2+-H(3) O(4) P(15): 894.90; y2+-H(3) O(4) P(14): 837.88; y2+-H(3) O(4) P(13): 787.35; y2+-H(3) O(4) P(12): 730.33; y2+-H(3) O(4) P(11): 673.31; y2+-H(3) O(4) P(10): 616.29; y1+(9): 1162.55","ptmRS..Best.Site.Probabilities":"S14(Phospho): 99.44","ptmRS..Phospho.Site.Probabilities":"S(3): 0.0; T(10): 0.0; S(14): 99.4; T(16): 0.6"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (L4)","Search.ID":"L","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].msEGITDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S2(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":3,"DeltaScore":0.1584,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1096.46611,"Contaminant":"False","Aligned.m.z..Da.":1096.46597,"MH...Da.":2191.92495,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":-1.32,"Deltam.z..Da.":-0.00145,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":19536346,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":8.00233,"Ion.Inject.Time..ms.":22,"RT..min.":15.7929,"First.Scan":15649,"Last.Scan":15649,"Master.Scan.s.":15642,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_01.raw","File.ID":"F25","Quan.Info":"","Peptides.Matched":1913,"XCorr":3.85,"X..Protein.Groups":1,"Percolator.q.Value":0.00033,"Percolator.PEP":9.005e-08,"Percolator.SVMScore":2.935,"Precursor.Area":16847080,"Apex.RT..min.":15.79,"ptmRS..Binomial.Peptide.Score":417.78,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(2): 112.04; b1+-H(3) O(4) P(3): 241.08; b1+-H(3) O(4) P(4): 298.10; b1+-H(3) O(4) P(5): 411.19; b1+-H(3) O(4) P(6): 512.24; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+-H(3) O(4) P(9): 869.39; b1+-H(3) O(4) P(10): 998.43; b1+-H(3) O(4) P(16): 1669.76; y1+(14): 1683.77","ptmRS..Best.Site.Probabilities":"S2(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 100.0; T(6): 0.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (L4)","Search.ID":"L","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0588,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40391,"Contaminant":"False","Aligned.m.z..Da.":967.40376,"MH...Da.":2900.19718,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":-0.74,"Deltam.z..Da.":-0.00072,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":248719.35938,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":16.5611,"First.Scan":16589,"Last.Scan":16589,"Master.Scan.s.":16585,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_01.raw","File.ID":"F25","Quan.Info":"","Peptides.Matched":2388,"XCorr":4.25,"X..Protein.Groups":1,"Percolator.q.Value":0.00033,"Percolator.PEP":1.776e-09,"Percolator.SVMScore":3.702,"ptmRS..Binomial.Peptide.Score":360.65,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y2+-H(3) O(4) P(14): 811.37; b1+(12): 1344.56","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (L4)","Search.ID":"L","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DIFSNDELLSDAYDAK","Annotated.Sequence":"[K].DIFSNDELLSDAYDAK.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7264,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":908.42038,"Contaminant":"False","Aligned.m.z..Da.":908.42031,"MH...Da.":1815.8335,"Theo..MH...Da.":1815.82831,"DeltaM..ppm.":2.85,"Deltam.z..Da.":0.00259,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":743642.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":6.02901,"Ion.Inject.Time..ms.":22,"RT..min.":17.0518,"First.Scan":17237,"Last.Scan":17237,"Master.Scan.s.":17234,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_01.raw","File.ID":"F25","Quan.Info":"","Peptides.Matched":1743,"XCorr":4.35,"X..Protein.Groups":1,"Percolator.q.Value":0.00033,"Percolator.PEP":3.935e-10,"Percolator.SVMScore":3.996,"Precursor.Area":3175823,"Apex.RT..min.":17.08,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (L4)","Search.ID":"L","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.4282,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40871,"Contaminant":"False","Aligned.m.z..Da.":967.40863,"MH...Da.":2900.21159,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":4.23,"Deltam.z..Da.":0.00409,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":257459.17188,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":7.41627,"Ion.Inject.Time..ms.":22,"RT..min.":17.0512,"First.Scan":17236,"Last.Scan":17236,"Master.Scan.s.":17234,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_01.raw","File.ID":"F25","Quan.Info":"","Peptides.Matched":2677,"XCorr":3.62,"X..Protein.Groups":1,"Percolator.q.Value":0.00033,"Percolator.PEP":1.249e-07,"Percolator.SVMScore":2.871,"ptmRS..Binomial.Peptide.Score":335.69,"ptmRS..Isoform.Confidence.Probability":0.5,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"Y12(Phospho): 50; S13(Phospho): 50","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 50.0; S(13): 50.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (L4)","Search.ID":"L","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.4108,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40362,"Contaminant":"False","Aligned.m.z..Da.":967.40371,"MH...Da.":2900.19631,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":-1.04,"Deltam.z..Da.":-0.00101,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":226109.59375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":13.93659,"Ion.Inject.Time..ms.":22,"RT..min.":17.5139,"First.Scan":17876,"Last.Scan":17876,"Master.Scan.s.":17874,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_01.raw","File.ID":"F25","Quan.Info":"","Peptides.Matched":2333,"XCorr":4.26,"X..Protein.Groups":1,"Percolator.q.Value":0.00033,"Percolator.PEP":5.374e-08,"Percolator.SVMScore":3.036,"ptmRS..Binomial.Peptide.Score":317.3,"ptmRS..Isoform.Confidence.Probability":0.5,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"Y12(Phospho): 50; S13(Phospho): 50","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 50.0; S(13): 50.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (L4)","Search.ID":"L","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].mSEGITDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7656,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1056.48349,"Contaminant":"False","Aligned.m.z..Da.":1056.48365,"MH...Da.":2111.95971,"Theo..MH...Da.":2111.96151,"DeltaM..ppm.":-0.85,"Deltam.z..Da.":-0.0009,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":396673.9375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":89.19552,"Ion.Inject.Time..ms.":22,"RT..min.":14.6432,"First.Scan":14258,"Last.Scan":14258,"Master.Scan.s.":14250,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_01.raw","File.ID":"F25","Quan.Info":"","Peptides.Matched":2289,"XCorr":4.65,"X..Protein.Groups":1,"Percolator.q.Value":0.00033,"Percolator.PEP":7.382e-13,"Percolator.SVMScore":5.223,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (L4)","Search.ID":"L","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":2,"DeltaScore":0.0022,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40597,"Contaminant":"False","Aligned.m.z..Da.":967.40591,"MH...Da.":2900.20336,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":1.39,"Deltam.z..Da.":0.00134,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":984883.4375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":28.8882,"Ion.Inject.Time..ms.":22,"RT..min.":15.4379,"First.Scan":15223,"Last.Scan":15223,"Master.Scan.s.":15212,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_01.raw","File.ID":"F25","Quan.Info":"","Peptides.Matched":2580,"XCorr":4.59,"X..Protein.Groups":1,"Percolator.q.Value":0.00033,"Percolator.PEP":2.941e-07,"Percolator.SVMScore":2.703,"Precursor.Area":32549064,"Apex.RT..min.":15.09,"ptmRS..Binomial.Peptide.Score":289.49,"ptmRS..Isoform.Confidence.Probability":0.5,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"Y12(Phospho): 50; S13(Phospho): 50","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 50.0; S(13): 50.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (L4)","Search.ID":"L","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0238,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40465,"Contaminant":"False","Aligned.m.z..Da.":967.40457,"MH...Da.":2900.1994,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":0.03,"Deltam.z..Da.":0.00002,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":957398.4375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":9.09385,"Ion.Inject.Time..ms.":22,"RT..min.":15.5174,"First.Scan":15317,"Last.Scan":15317,"Master.Scan.s.":15316,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_01.raw","File.ID":"F25","Quan.Info":"","Peptides.Matched":2477,"XCorr":5.05,"X..Protein.Groups":1,"Percolator.q.Value":0.00033,"Percolator.PEP":2.021e-10,"Percolator.SVMScore":4.126,"Precursor.Area":32549064,"Apex.RT..min.":15.09,"ptmRS..Binomial.Peptide.Score":361.17,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(16): 1771.69; b1+(12): 1344.56","ptmRS..Best.Site.Probabilities":"S13(Phospho): 99.99","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (L4)","Search.ID":"L","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FFISNPDLVDRLEK","Annotated.Sequence":"[R].FFISNPDLVDRLEK.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q03558","Master.Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","Protein.Accessions":"Q03558","Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.7491,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":564.97022,"Contaminant":"False","Aligned.m.z..Da.":564.9702,"MH...Da.":1692.89611,"Theo..MH...Da.":1692.89555,"DeltaM..ppm.":0.33,"Deltam.z..Da.":0.00019,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":446995.34375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":29.48943,"Ion.Inject.Time..ms.":22,"RT..min.":15.3094,"First.Scan":15066,"Last.Scan":15066,"Master.Scan.s.":15056,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_01.raw","File.ID":"F25","Quan.Info":"","Peptides.Matched":541,"XCorr":2.79,"X..Protein.Groups":1,"Percolator.q.Value":0.00033,"Percolator.PEP":8.605e-09,"Percolator.SVMScore":3.393,"Precursor.Area":330203.46875,"Apex.RT..min.":15.31,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (L4)","Search.ID":"L","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].mSEGItDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); T6(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.161,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":731.31509,"Contaminant":"False","Aligned.m.z..Da.":731.31503,"MH...Da.":2191.93073,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":1.32,"Deltam.z..Da.":0.00096,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":268258.71875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":15.5505,"First.Scan":15357,"Last.Scan":15357,"Master.Scan.s.":15355,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_01.raw","File.ID":"F25","Quan.Info":"","Peptides.Matched":2086,"XCorr":3.54,"X..Protein.Groups":1,"Percolator.q.Value":0.00033,"Percolator.PEP":1.945e-07,"Percolator.SVMScore":2.784,"Precursor.Area":227705.76562,"Apex.RT..min.":15.54,"ptmRS..Binomial.Peptide.Score":390.77,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(6): 512.24; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+-H(3) O(4) P(9): 869.39; b1+-H(3) O(4) P(10): 998.43; b1+(2): 130.05; b1+(3): 259.09; b1+(4): 316.11; b1+(5): 429.20; b2+(3): 130.05","ptmRS..Best.Site.Probabilities":"T6(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; T(6): 100.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (L4)","Search.ID":"L","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].mSEGItDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); T6(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.0943,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1096.468,"Contaminant":"False","Aligned.m.z..Da.":1096.4679,"MH...Da.":2191.92872,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":0.4,"Deltam.z..Da.":0.00044,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":432046.84375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":15.5287,"First.Scan":15331,"Last.Scan":15331,"Master.Scan.s.":15329,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_01.raw","File.ID":"F25","Quan.Info":"","Peptides.Matched":1965,"XCorr":5.3,"X..Protein.Groups":1,"Percolator.q.Value":0.00033,"Percolator.PEP":1.648e-11,"Percolator.SVMScore":4.616,"Precursor.Area":362621.84375,"Apex.RT..min.":15.53,"ptmRS..Binomial.Peptide.Score":478.66,"ptmRS..Isoform.Confidence.Probability":0.996,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(6): 512.24; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+-H(3) O(4) P(9): 869.39; b1+-H(3) O(4) P(10): 998.43; b1+-H(3) O(4) P(11): 1085.46; b1+(3): 259.09; b1+(4): 316.11; b1+(5): 429.20","ptmRS..Best.Site.Probabilities":"T6(Phospho): 99.63","ptmRS..Phospho.Site.Probabilities":"S(2): 0.4; T(6): 99.6; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (L4)","Search.ID":"L","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].mSEGITDIEEsQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S11(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1253,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1096.4657,"Contaminant":"False","Aligned.m.z..Da.":1096.46576,"MH...Da.":2191.92412,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":-1.7,"Deltam.z..Da.":-0.00186,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":338382.8125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":14.9976,"First.Scan":14681,"Last.Scan":14681,"Master.Scan.s.":14679,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_01.raw","File.ID":"F25","Quan.Info":"","Peptides.Matched":1867,"XCorr":3.91,"X..Protein.Groups":1,"Percolator.q.Value":0.00033,"Percolator.PEP":2.95e-08,"Percolator.SVMScore":3.153,"ptmRS..Binomial.Peptide.Score":297.5,"ptmRS..Isoform.Confidence.Probability":0.995,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(14): 1665.76; y1+-H(3) O(4) P(11): 1336.60; y1+-H(3) O(4) P(10): 1207.56; b1+(6): 530.25; b1+(8): 758.36","ptmRS..Best.Site.Probabilities":"S11(Phospho): 99.46","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; T(6): 0.5; S(11): 99.5; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (L4)","Search.ID":"L","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FTVSAIYSDLPQQER","Annotated.Sequence":"[K].FTVSAIYSDLPQQER.[D]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7051,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":877.44187,"Contaminant":"False","Aligned.m.z..Da.":877.44191,"MH...Da.":1753.87647,"Theo..MH...Da.":1753.87554,"DeltaM..ppm.":0.53,"Deltam.z..Da.":0.00046,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":494616.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":28.38132,"Ion.Inject.Time..ms.":22,"RT..min.":15.0093,"First.Scan":14696,"Last.Scan":14696,"Master.Scan.s.":14692,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_01.raw","File.ID":"F25","Quan.Info":"","Peptides.Matched":1063,"XCorr":3.9,"X..Protein.Groups":1,"Percolator.q.Value":0.00033,"Percolator.PEP":1.138e-09,"Percolator.SVMScore":3.789,"Precursor.Area":984173.75,"Apex.RT..min.":15.03,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (L4)","Search.ID":"L","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"GVFGYGFEEPSAIQQR","Annotated.Sequence":"[R].GVFGYGFEEPSAIQQR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7268,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":892.93331,"Contaminant":"False","Aligned.m.z..Da.":892.93335,"MH...Da.":1784.85935,"Theo..MH...Da.":1784.86022,"DeltaM..ppm.":-0.49,"Deltam.z..Da.":-0.00044,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":569987.6875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":16.20172,"Ion.Inject.Time..ms.":22,"RT..min.":15.01,"First.Scan":14697,"Last.Scan":14697,"Master.Scan.s.":14692,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_01.raw","File.ID":"F25","Quan.Info":"","Peptides.Matched":1278,"XCorr":3.99,"X..Protein.Groups":1,"Percolator.q.Value":0.00033,"Percolator.PEP":9.383e-11,"Percolator.SVMScore":4.276,"Precursor.Area":1276361.25,"Apex.RT..min.":15.03,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (L4)","Search.ID":"L","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":4,"DeltaScore":0.0691,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40519,"Contaminant":"False","Aligned.m.z..Da.":967.40519,"MH...Da.":2900.20102,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":0.58,"Deltam.z..Da.":0.00056,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":6942714,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":41.62066,"Ion.Inject.Time..ms.":22,"RT..min.":15.177,"First.Scan":14903,"Last.Scan":14903,"Master.Scan.s.":14900,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_01.raw","File.ID":"F25","Quan.Info":"","Peptides.Matched":2518,"XCorr":5.5,"X..Protein.Groups":1,"Percolator.q.Value":0.00033,"Percolator.PEP":4.256e-11,"Percolator.SVMScore":4.431,"Precursor.Area":32549064,"Apex.RT..min.":15.09,"ptmRS..Binomial.Peptide.Score":655.36,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(13): 1413.58; b1+-H(3) O(4) P(14): 1528.61; b1+-H(3) O(4) P(15): 1642.65; b1+-H(3) O(4) P(16): 1771.69; y1+-H(3) O(4) P(15): 1750.78; y1+-H(3) O(4) P(14): 1621.73; y1+-H(3) O(4) P(13): 1458.67; b2+-H(3) O(4) P(16): 886.35; b2+-H(3) O(4) P(18): 1014.90; y2+-H(3) O(4) P(14): 811.37; b1+(12): 1344.56; y1+(13): 1556.65","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (L4)","Search.ID":"L","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":5,"DeltaScore":0.07,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40427,"Contaminant":"False","Aligned.m.z..Da.":967.40429,"MH...Da.":2900.19825,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":-0.37,"Deltam.z..Da.":-0.00036,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":20379588,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":3.48133,"Ion.Inject.Time..ms.":7.569,"RT..min.":15.1135,"First.Scan":14825,"Last.Scan":14825,"Master.Scan.s.":14822,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_01.raw","File.ID":"F25","Quan.Info":"","Peptides.Matched":2452,"XCorr":7,"X..Protein.Groups":1,"Percolator.q.Value":0.00033,"Percolator.PEP":2.402e-14,"Percolator.SVMScore":5.892,"Precursor.Area":32549064,"Apex.RT..min.":15.09,"ptmRS..Binomial.Peptide.Score":745.82,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(13): 1413.58; b1+-H(3) O(4) P(14): 1528.61; b1+-H(3) O(4) P(15): 1642.65; b1+-H(3) O(4) P(16): 1771.69; y1+-H(3) O(4) P(15): 1750.78; y1+-H(3) O(4) P(14): 1621.73; y1+-H(3) O(4) P(13): 1458.67; b2+-H(3) O(4) P(16): 886.35; b2+-H(3) O(4) P(18): 1014.90; y2+-H(3) O(4) P(14): 811.37; b1+(12): 1344.56; y1+(13): 1556.65","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (L4)","Search.ID":"L","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"HSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[R].HSDsYsENETNHTNVPISSTGGTNNK.[T]","Modifications":"S4(Phospho); S6(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.1503,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":984.05598,"Contaminant":"False","Aligned.m.z..Da.":984.05613,"MH...Da.":2950.1534,"Theo..MH...Da.":2950.15241,"DeltaM..ppm.":0.33,"Deltam.z..Da.":0.00033,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":4091257.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":8.0259,"First.Scan":5954,"Last.Scan":5954,"Master.Scan.s.":5950,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_01.raw","File.ID":"F25","Quan.Info":"","Peptides.Matched":1171,"XCorr":6.92,"X..Protein.Groups":1,"Percolator.q.Value":0.00033,"Percolator.PEP":1.423e-09,"Percolator.SVMScore":3.745,"Precursor.Area":4701722.5,"Apex.RT..min.":8.03,"ptmRS..Binomial.Peptide.Score":622.39,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(6): 739.21; y2+-H(3) O(4) P(22): 1173.53; y2+-H(3) O(4) P(21): 1092.00; b1+- -2H(3) O(4) P -2H(3) O(4) P(6): 641.23; b1+- -2H(3) O(4) P -2H(3) O(4) P(11): 1228.45; b1+- -2H(3) O(4) P -2H(3) O(4) P(15): 1679.67; b2+- -2H(3) O(4) P -2H(3) O(4) P(13): 733.78; b2+- -2H(3) O(4) P -2H(3) O(4) P(14): 790.80; b2+- -2H(3) O(4) P -2H(3) O(4) P(15): 840.34","ptmRS..Best.Site.Probabilities":"S4(Phospho): 100; S6(Phospho): 99.99","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(4): 100.0; Y(5): 0.0; S(6): 100.0; T(10): 0.0; T(13): 0.0; S(18): 0.0; S(19): 0.0; T(20): 0.0; T(23): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (L4)","Search.ID":"L","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"HSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[R].HSDSySENETNHTNVPISSTGGTNNK.[T]","Modifications":"Y5(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":0,"Charge":4,"Original.Precursor.Charge":4,"DeltaScore":0.0065,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":718.30161,"Contaminant":"False","Aligned.m.z..Da.":718.30168,"MH...Da.":2870.1846,"Theo..MH...Da.":2870.18608,"DeltaM..ppm.":-0.51,"Deltam.z..Da.":-0.00037,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":5889340,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":7.6676,"First.Scan":5460,"Last.Scan":5460,"Master.Scan.s.":5456,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_01.raw","File.ID":"F25","Quan.Info":"","Peptides.Matched":2477,"XCorr":6.13,"X..Protein.Groups":1,"Percolator.q.Value":0.00033,"Percolator.PEP":7.536e-10,"Percolator.SVMScore":3.869,"Precursor.Area":7406350.5,"Apex.RT..min.":7.67,"ptmRS..Binomial.Peptide.Score":300.88,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(9): 1031.37; b1+-H(3) O(4) P(10): 1132.42; b1+-H(3) O(4) P(11): 1246.46; b1+-H(3) O(4) P(15): 1697.68; b2+-H(3) O(4) P(7): 394.65; b2+-H(3) O(4) P(13): 742.79; b2+-H(3) O(4) P(14): 799.81; b2+-H(3) O(4) P(15): 849.34; b2+-H(3) O(4) P(17): 954.41; b1+(4): 427.16","ptmRS..Best.Site.Probabilities":"S6(Phospho): 99.35","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(4): 0.7; Y(5): 0.0; S(6): 99.4; T(10): 0.0; T(13): 0.0; S(18): 0.0; S(19): 0.0; T(20): 0.0; T(23): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (L4)","Search.ID":"L","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"AAEAGETGAATSATEGDNNNNTAAGDKK","Annotated.Sequence":"[K].AAEAGETGAATsATEGDNNNNTAAGDKK.[G]","Modifications":"S12(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0216,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":906.04945,"Contaminant":"False","Aligned.m.z..Da.":906.04946,"MH...Da.":2716.1338,"Theo..MH...Da.":2716.13298,"DeltaM..ppm.":0.3,"Deltam.z..Da.":0.00027,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":8130335,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":1.42039,"Ion.Inject.Time..ms.":21.487,"RT..min.":5.0262,"First.Scan":1780,"Last.Scan":1780,"Master.Scan.s.":1777,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_01.raw","File.ID":"F25","Quan.Info":"","Peptides.Matched":2558,"XCorr":7.41,"X..Protein.Groups":1,"Percolator.q.Value":0.00033,"Percolator.PEP":8.382e-16,"Percolator.SVMScore":6.997,"ptmRS..Binomial.Peptide.Score":583.85,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(15): 1300.57; y1+-H(3) O(4) P(18): 1789.79; y1+-H(3) O(4) P(17): 1688.75; y2+-H(3) O(4) P(25): 1174.02; y2+-H(3) O(4) P(24): 1138.50; y2+-H(3) O(4) P(22): 1045.47; y2+-H(3) O(4) P(18): 895.40; y2+-H(3) O(4) P(17): 844.88","ptmRS..Best.Site.Probabilities":"S12(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"T(7): 0.0; T(11): 0.0; S(12): 100.0; T(14): 0.0; T(22): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (L4)","Search.ID":"L","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KDELTLEGIK","Annotated.Sequence":"[K].KDELTLEGIK.[Q]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.53,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":573.32428,"Contaminant":"False","Aligned.m.z..Da.":573.3242,"MH...Da.":1145.64128,"Theo..MH...Da.":1145.64123,"DeltaM..ppm.":0.04,"Deltam.z..Da.":0.00002,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3114047,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":4.74222,"Ion.Inject.Time..ms.":22,"RT..min.":10.2351,"First.Scan":8504,"Last.Scan":8504,"Master.Scan.s.":8498,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_01.raw","File.ID":"F25","Quan.Info":"","Peptides.Matched":307,"XCorr":4,"X..Protein.Groups":1,"Percolator.q.Value":0.00033,"Percolator.PEP":1.198e-08,"Percolator.SVMScore":3.329,"Precursor.Area":3387242.25,"Apex.RT..min.":10.25,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (L4)","Search.ID":"L","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"APQALMLAPTR","Annotated.Sequence":"[K].APQALmLAPTR.[E]","Modifications":"M6(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.731,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":592.82654,"Contaminant":"False","Aligned.m.z..Da.":592.82646,"MH...Da.":1184.64581,"Theo..MH...Da.":1184.6456,"DeltaM..ppm.":0.17,"Deltam.z..Da.":0.0001,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2243858.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":13.3586,"Ion.Inject.Time..ms.":22,"RT..min.":10.1725,"First.Scan":8430,"Last.Scan":8430,"Master.Scan.s.":8420,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_01.raw","File.ID":"F25","Quan.Info":"","Peptides.Matched":304,"XCorr":3.16,"X..Protein.Groups":1,"Percolator.q.Value":0.00033,"Percolator.PEP":3.914e-07,"Percolator.SVMScore":2.648,"Precursor.Area":3412768.5,"Apex.RT..min.":10.19,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (L4)","Search.ID":"L","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"DFRHSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[K].DFRHsDsYSENETNHTNVPISSTGGTNNK.[T]","Modifications":"S5(Phospho); S7(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0441,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1123.4552,"Contaminant":"False","Aligned.m.z..Da.":1123.45533,"MH...Da.":3368.35106,"Theo..MH...Da.":3368.34888,"DeltaM..ppm.":0.65,"Deltam.z..Da.":0.00073,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1243423.875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":8.6609,"First.Scan":6701,"Last.Scan":6701,"Master.Scan.s.":6691,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_01.raw","File.ID":"F25","Quan.Info":"","Peptides.Matched":983,"XCorr":5.89,"X..Protein.Groups":1,"Percolator.q.Value":0.00033,"Percolator.PEP":1.34e-08,"Percolator.SVMScore":3.307,"ptmRS..Binomial.Peptide.Score":578.71,"ptmRS..Isoform.Confidence.Probability":0.5,"ptmRS..Isoform.Group.Report":"y2+-H(3) O(4) P(24): 1274.56; y2+-H(3) O(4) P(23): 1217.05; b1+- -2H(3) O(4) P -2H(3) O(4) P(12): 1431.56; b1+- -2H(3) O(4) P -2H(3) O(4) P(13): 1532.60; b1+- -2H(3) O(4) P -2H(3) O(4) P(14): 1646.65; b2+- -2H(3) O(4) P -2H(3) O(4) P(15): 892.36; b2+- -2H(3) O(4) P -2H(3) O(4) P(16): 942.88; b2+- -2H(3) O(4) P -2H(3) O(4) P(17): 999.90; b2+- -2H(3) O(4) P -2H(3) O(4) P(18): 1049.44; b2+- -2H(3) O(4) P -2H(3) O(4) P(20): 1154.50","ptmRS..Best.Site.Probabilities":"S5(Phospho): 100; S7(Phospho): 50; S9(Phospho): 50","ptmRS..Phospho.Site.Probabilities":"S(5): 100.0; S(7): 50.0; Y(8): 0.0; S(9): 50.0; T(13): 0.0; T(16): 0.0; S(21): 0.0; S(22): 0.0; T(23): 0.0; T(26): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AB4)","Search.ID":"AB","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"AIMPIIEGHDVLAQAQSGTGK","Annotated.Sequence":"[R].AImPIIEGHDVLAQAQSGTGK.[T]","Modifications":"M3(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.7175,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":718.04099,"Contaminant":"False","Aligned.m.z..Da.":718.04101,"MH...Da.":2152.10844,"Theo..MH...Da.":2152.10668,"DeltaM..ppm.":0.82,"Deltam.z..Da.":0.00059,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":386736.28125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":3.83783,"Ion.Inject.Time..ms.":22,"RT..min.":12.7935,"First.Scan":12014,"Last.Scan":12014,"Master.Scan.s.":12005,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_03.raw","File.ID":"F9","Quan.Info":"","Peptides.Matched":1001,"XCorr":3.08,"X..Protein.Groups":1,"Percolator.q.Value":0.00034,"Percolator.PEP":2.908e-08,"Percolator.SVMScore":2.867,"Precursor.Area":387695.46875,"Apex.RT..min.":12.79,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AB4)","Search.ID":"AB","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KFQDDTLNR","Annotated.Sequence":"[K].KFQDDtLNR.[V]","Modifications":"T6(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6606,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":608.77178,"Contaminant":"False","Aligned.m.z..Da.":608.77175,"MH...Da.":1216.5363,"Theo..MH...Da.":1216.53579,"DeltaM..ppm.":0.41,"Deltam.z..Da.":0.00025,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1348619.375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":24.75276,"Ion.Inject.Time..ms.":22,"RT..min.":6.2105,"First.Scan":3175,"Last.Scan":3175,"Master.Scan.s.":3165,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_03.raw","File.ID":"F9","Quan.Info":"","Peptides.Matched":622,"XCorr":2.18,"X..Protein.Groups":1,"Percolator.q.Value":0.00034,"Percolator.PEP":0.00021,"Percolator.SVMScore":1.225,"Precursor.Area":797555.5,"Apex.RT..min.":6.2,"ptmRS..Binomial.Peptide.Score":303.8,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"T6(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"T(6): 100.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AB4)","Search.ID":"AB","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"IQDPQMTGYVSTR","Annotated.Sequence":"[R].IQDPQMTGyVSTR.[Y]","Modifications":"Y9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P32485","Master.Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","Protein.Accessions":"P32485","Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1066,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":788.34512,"Contaminant":"False","Aligned.m.z..Da.":788.34515,"MH...Da.":1575.68297,"Theo..MH...Da.":1575.68728,"DeltaM..ppm.":-2.74,"Deltam.z..Da.":-0.00216,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1631682,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":9.5652,"First.Scan":7778,"Last.Scan":7778,"Master.Scan.s.":7767,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_03.raw","File.ID":"F9","Quan.Info":"","Peptides.Matched":1147,"XCorr":2.72,"X..Protein.Groups":1,"Percolator.q.Value":0.00034,"Percolator.PEP":0.00001,"Percolator.SVMScore":1.745,"Precursor.Area":1408306,"Apex.RT..min.":9.57,"ptmRS..Binomial.Peptide.Score":283.23,"ptmRS..Isoform.Confidence.Probability":0.989,"ptmRS..Isoform.Group.Report":"y1+(6): 762.32; y1+(5): 705.30","ptmRS..Best.Site.Probabilities":"Y9(Phospho): 98.9","ptmRS..Phospho.Site.Probabilities":"T(7): 0.4; Y(9): 98.9; S(11): 0.4; T(12): 0.4"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AB4)","Search.ID":"AB","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MGSINNNFNTNNNSNTDLDRDWK","Annotated.Sequence":"[-].mGSINNNFNTNNNSNtDLDRDWK.[T]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); T16(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P39517","Master.Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","Protein.Accessions":"P39517","Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0604,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":892.37691,"Contaminant":"False","Aligned.m.z..Da.":892.37691,"MH...Da.":2675.1162,"Theo..MH...Da.":2675.11179,"DeltaM..ppm.":1.65,"Deltam.z..Da.":0.00147,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":586423.5625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":9.2126,"Ion.Inject.Time..ms.":22,"RT..min.":13.588,"First.Scan":13033,"Last.Scan":13033,"Master.Scan.s.":13030,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_03.raw","File.ID":"F9","Quan.Info":"","Peptides.Matched":2374,"XCorr":2.65,"X..Protein.Groups":1,"Percolator.q.Value":0.00034,"Percolator.PEP":0.00046,"Percolator.SVMScore":1.086,"Precursor.Area":1594136,"Apex.RT..min.":13.56,"ptmRS..Binomial.Peptide.Score":289.36,"ptmRS..Isoform.Confidence.Probability":0.5,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(12): 1459.66; y2+-H(3) O(4) P(18): 1082.48; y2+-H(3) O(4) P(17): 1025.45; y2+-H(3) O(4) P(16): 968.43; y2+-H(3) O(4) P(15): 894.90; y2+-H(3) O(4) P(14): 837.88; y2+-H(3) O(4) P(12): 730.33; y2+-H(3) O(4) P(10): 616.29","ptmRS..Best.Site.Probabilities":"S14(Phospho): 50; T16(Phospho): 50","ptmRS..Phospho.Site.Probabilities":"S(3): 0.0; T(10): 0.0; S(14): 50.0; T(16): 50.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AB4)","Search.ID":"AB","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"AAEAGETGAATSATEGDNNNNTAAGDKK","Annotated.Sequence":"[K].AAEAGETGAATsATEGDNNNNTAAGDKK.[G]","Modifications":"S12(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0101,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":906.04961,"Contaminant":"False","Aligned.m.z..Da.":906.04952,"MH...Da.":2716.1343,"Theo..MH...Da.":2716.13298,"DeltaM..ppm.":0.48,"Deltam.z..Da.":0.00044,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1867818.125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":3.96919,"Ion.Inject.Time..ms.":22,"RT..min.":5.0314,"First.Scan":1793,"Last.Scan":1793,"Master.Scan.s.":1787,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_03.raw","File.ID":"F9","Quan.Info":"","Peptides.Matched":2561,"XCorr":5.97,"X..Protein.Groups":1,"Percolator.q.Value":0.00034,"Percolator.PEP":1.691e-14,"Percolator.SVMScore":5.512,"ptmRS..Binomial.Peptide.Score":490.27,"ptmRS..Isoform.Confidence.Probability":0.985,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(15): 1300.57; y1+-H(3) O(4) P(18): 1789.79; y2+-H(3) O(4) P(25): 1174.02; y2+-H(3) O(4) P(24): 1138.50; y2+-H(3) O(4) P(22): 1045.47; y2+-H(3) O(4) P(18): 895.40; y2+-H(3) O(4) P(17): 844.88","ptmRS..Best.Site.Probabilities":"S12(Phospho): 98.52","ptmRS..Phospho.Site.Probabilities":"T(7): 0.7; T(11): 0.7; S(12): 98.5; T(14): 0.0; T(22): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AB4)","Search.ID":"AB","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"IQDPQMTGYVSTR","Annotated.Sequence":"[R].IQDPQmTGyVSTR.[Y]","Modifications":"M6(Oxidation); Y9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P32485","Master.Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","Protein.Accessions":"P32485","Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1307,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":796.34622,"Contaminant":"False","Aligned.m.z..Da.":796.34627,"MH...Da.":1591.68516,"Theo..MH...Da.":1591.6822,"DeltaM..ppm.":1.86,"Deltam.z..Da.":0.00148,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2015113.875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":7.6879,"First.Scan":5562,"Last.Scan":5562,"Master.Scan.s.":5557,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_03.raw","File.ID":"F9","Quan.Info":"","Peptides.Matched":1151,"XCorr":2.83,"X..Protein.Groups":1,"Percolator.q.Value":0.00034,"Percolator.PEP":0.00001,"Percolator.SVMScore":1.75,"Precursor.Area":1570433.25,"Apex.RT..min.":7.69,"ptmRS..Binomial.Peptide.Score":247.11,"ptmRS..Isoform.Confidence.Probability":0.498,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(10): 1137.54","ptmRS..Best.Site.Probabilities":"T7(Phospho): 49.75; Y9(Phospho): 49.75","ptmRS..Phospho.Site.Probabilities":"T(7): 49.8; Y(9): 49.8; S(11): 0.3; T(12): 0.3"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AB4)","Search.ID":"AB","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSSPLHRLSPTGK","Annotated.Sequence":"[R].mSsPLHRLsPTGK.[S]","Modifications":"M1(Oxidation); S3(Phospho); S9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.4746,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":529.56519,"Contaminant":"False","Aligned.m.z..Da.":529.56523,"MH...Da.":1586.68103,"Theo..MH...Da.":1586.67977,"DeltaM..ppm.":0.79,"Deltam.z..Da.":0.00042,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1561564.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":55.55368,"Ion.Inject.Time..ms.":22,"RT..min.":7.7364,"First.Scan":5632,"Last.Scan":5632,"Master.Scan.s.":5622,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_03.raw","File.ID":"F9","Quan.Info":"","Peptides.Matched":1071,"XCorr":3.54,"X..Protein.Groups":1,"Percolator.q.Value":0.00034,"Percolator.PEP":0.00016,"Percolator.SVMScore":1.278,"Precursor.Area":1505443.875,"Apex.RT..min.":7.75,"ptmRS..Binomial.Peptide.Score":308.62,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(8): 877.50; y1+-H(3) O(4) P(7): 740.44; y2+-H(3) O(4) P(12): 671.34; y2+-H(3) O(4) P(11): 627.82; y2+-H(3) O(4) P(10): 544.32; y2+-H(3) O(4) P(9): 495.80; y2+-H(3) O(4) P(8): 439.25; y1+(4): 402.23; y1+(3): 305.18","ptmRS..Best.Site.Probabilities":"S3(Phospho): 100; S9(Phospho): 99.99","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(3): 100.0; S(9): 100.0; T(11): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AB4)","Search.ID":"AB","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"HSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[R].HSDSySENETNHTNVPISSTGGTNNK.[T]","Modifications":"Y5(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":0,"Charge":4,"Original.Precursor.Charge":4,"DeltaScore":0.015,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":718.30127,"Contaminant":"False","Aligned.m.z..Da.":718.30131,"MH...Da.":2870.18324,"Theo..MH...Da.":2870.18608,"DeltaM..ppm.":-0.99,"Deltam.z..Da.":-0.00071,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2237954.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":7.6705,"First.Scan":5537,"Last.Scan":5537,"Master.Scan.s.":5531,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_03.raw","File.ID":"F9","Quan.Info":"","Peptides.Matched":2388,"XCorr":5.32,"X..Protein.Groups":1,"Percolator.q.Value":0.00034,"Percolator.PEP":4.227e-10,"Percolator.SVMScore":3.646,"Precursor.Area":1927199,"Apex.RT..min.":7.67,"ptmRS..Binomial.Peptide.Score":303.02,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(11): 1246.46; b2+-H(3) O(4) P(7): 394.65; b2+-H(3) O(4) P(13): 742.79; b2+-H(3) O(4) P(14): 799.81; b2+-H(3) O(4) P(15): 849.34; b2+-H(3) O(4) P(17): 954.41; b1+(4): 427.16","ptmRS..Best.Site.Probabilities":"S6(Phospho): 99.41","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(4): 0.6; Y(5): 0.0; S(6): 99.4; T(10): 0.0; T(13): 0.0; S(18): 0.0; S(19): 0.0; T(20): 0.0; T(23): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AB4)","Search.ID":"AB","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"HSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[R].HSDSySENETNHTNVPISSTGGTNNK.[T]","Modifications":"Y5(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0031,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":957.3994,"Contaminant":"False","Aligned.m.z..Da.":957.39945,"MH...Da.":2870.18364,"Theo..MH...Da.":2870.18608,"DeltaM..ppm.":-0.85,"Deltam.z..Da.":-0.00081,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2402519.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":7.6604,"First.Scan":5522,"Last.Scan":5522,"Master.Scan.s.":5518,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_03.raw","File.ID":"F9","Quan.Info":"","Peptides.Matched":2442,"XCorr":6.44,"X..Protein.Groups":1,"Percolator.q.Value":0.00034,"Percolator.PEP":1.925e-13,"Percolator.SVMScore":5.064,"Precursor.Area":4229503,"Apex.RT..min.":7.67,"ptmRS..Binomial.Peptide.Score":345.96,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(4): 409.15; b1+-H(3) O(4) P(5): 572.21; b1+-H(3) O(4) P(6): 659.24; b1+-H(3) O(4) P(10): 1132.42; b1+-H(3) O(4) P(11): 1246.46; b1+-H(3) O(4) P(14): 1598.61; b1+-H(3) O(4) P(15): 1697.68; b2+-H(3) O(4) P(12): 692.26; b2+-H(3) O(4) P(13): 742.79; b2+-H(3) O(4) P(14): 799.81; b2+-H(3) O(4) P(15): 849.34; y2+-H(3) O(4) P(25): 1318.08; y2+-H(3) O(4) P(24): 1274.56","ptmRS..Best.Site.Probabilities":"S4(Phospho): 99.39","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(4): 99.4; Y(5): 0.0; S(6): 0.6; T(10): 0.0; T(13): 0.0; S(18): 0.0; S(19): 0.0; T(20): 0.0; T(23): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AB4)","Search.ID":"AB","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DIFSNDELLSDAYDAK","Annotated.Sequence":"[K].DIFSNDELLSDAYDAK.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.4607,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":605.94705,"Contaminant":"False","Aligned.m.z..Da.":605.9467,"MH...Da.":1815.82661,"Theo..MH...Da.":1815.82831,"DeltaM..ppm.":-0.94,"Deltam.z..Da.":-0.00057,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":257399.07812,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":5.42981,"Ion.Inject.Time..ms.":22,"RT..min.":17.0909,"First.Scan":17074,"Last.Scan":17074,"Master.Scan.s.":17070,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_03.raw","File.ID":"F9","Quan.Info":"","Peptides.Matched":1621,"XCorr":1.78,"X..Protein.Groups":1,"Percolator.q.Value":0.00034,"Percolator.PEP":0.00015,"Percolator.SVMScore":1.294,"Precursor.Area":186187.26562,"Apex.RT..min.":17.09,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AB4)","Search.ID":"AB","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MFILDEADEMLSSGFK","Annotated.Sequence":"[K].mFILDEADEmLSSGFK.[E]","Modifications":"M1(Oxidation); M10(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6278,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":932.91738,"Contaminant":"False","Aligned.m.z..Da.":932.9169,"MH...Da.":1864.82749,"Theo..MH...Da.":1864.83433,"DeltaM..ppm.":-3.67,"Deltam.z..Da.":-0.00342,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":181923.90625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":32.68987,"Ion.Inject.Time..ms.":22,"RT..min.":16.6851,"First.Scan":16634,"Last.Scan":16634,"Master.Scan.s.":16628,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_03.raw","File.ID":"F9","Quan.Info":"","Peptides.Matched":1671,"XCorr":2.23,"X..Protein.Groups":1,"Percolator.q.Value":0.00034,"Percolator.PEP":1.487e-07,"Percolator.SVMScore":2.566,"Precursor.Area":194833.92188,"Apex.RT..min.":16.69,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AB4)","Search.ID":"AB","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FTVSAIYSDLPQQER","Annotated.Sequence":"[K].FTVSAIYSDLPQQER.[D]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.689,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":877.4415,"Contaminant":"False","Aligned.m.z..Da.":877.44159,"MH...Da.":1753.87572,"Theo..MH...Da.":1753.87554,"DeltaM..ppm.":0.11,"Deltam.z..Da.":0.00009,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":345816.625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":14.34948,"Ion.Inject.Time..ms.":22,"RT..min.":15.0387,"First.Scan":14716,"Last.Scan":14716,"Master.Scan.s.":14711,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_03.raw","File.ID":"F9","Quan.Info":"","Peptides.Matched":1047,"XCorr":3.44,"X..Protein.Groups":1,"Percolator.q.Value":0.00034,"Percolator.PEP":4.329e-07,"Percolator.SVMScore":2.369,"Precursor.Area":231396.28125,"Apex.RT..min.":15.04,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AB4)","Search.ID":"AB","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEySDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); Y12(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.4167,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.4031,"Contaminant":"False","Aligned.m.z..Da.":967.4032,"MH...Da.":2900.19476,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":-1.57,"Deltam.z..Da.":-0.00152,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":240999.46875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":15.0282,"First.Scan":14703,"Last.Scan":14703,"Master.Scan.s.":14698,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_03.raw","File.ID":"F9","Quan.Info":"","Peptides.Matched":2260,"XCorr":4.08,"X..Protein.Groups":1,"Percolator.q.Value":0.00034,"Percolator.PEP":5.032e-08,"Percolator.SVMScore":2.766,"Precursor.Area":9448331,"Apex.RT..min.":15.08,"ptmRS..Binomial.Peptide.Score":345.31,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(14): 1621.73; y2+-H(3) O(4) P(14): 811.37","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AB4)","Search.ID":"AB","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"GVFGYGFEEPSAIQQR","Annotated.Sequence":"[R].GVFGYGFEEPSAIQQR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6635,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":892.93427,"Contaminant":"False","Aligned.m.z..Da.":892.93437,"MH...Da.":1784.86127,"Theo..MH...Da.":1784.86022,"DeltaM..ppm.":0.59,"Deltam.z..Da.":0.00052,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":436333.5625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":16.18982,"Ion.Inject.Time..ms.":22,"RT..min.":15.0268,"First.Scan":14701,"Last.Scan":14701,"Master.Scan.s.":14698,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_03.raw","File.ID":"F9","Quan.Info":"","Peptides.Matched":1395,"XCorr":3.15,"X..Protein.Groups":1,"Percolator.q.Value":0.00034,"Percolator.PEP":2.988e-09,"Percolator.SVMScore":3.286,"Precursor.Area":332715.5,"Apex.RT..min.":15.04,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AB4)","Search.ID":"AB","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":5,"DeltaScore":0.0861,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40446,"Contaminant":"False","Aligned.m.z..Da.":967.40456,"MH...Da.":2900.19884,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":-0.17,"Deltam.z..Da.":-0.00016,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3313943.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":9.89118,"Ion.Inject.Time..ms.":22,"RT..min.":15.133,"First.Scan":14832,"Last.Scan":14832,"Master.Scan.s.":14828,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_03.raw","File.ID":"F9","Quan.Info":"","Peptides.Matched":2486,"XCorr":5.92,"X..Protein.Groups":1,"Percolator.q.Value":0.00034,"Percolator.PEP":1.526e-13,"Percolator.SVMScore":5.107,"Precursor.Area":9448331,"Apex.RT..min.":15.08,"ptmRS..Binomial.Peptide.Score":713.49,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(13): 1413.58; b1+-H(3) O(4) P(14): 1528.61; b1+-H(3) O(4) P(15): 1642.65; b1+-H(3) O(4) P(16): 1771.69; b1+-H(3) O(4) P(18): 2028.79; y1+-H(3) O(4) P(15): 1750.78; y1+-H(3) O(4) P(14): 1621.73; y1+-H(3) O(4) P(13): 1458.67; b2+-H(3) O(4) P(16): 886.35; b2+-H(3) O(4) P(17): 950.38; b2+-H(3) O(4) P(18): 1014.90; y2+-H(3) O(4) P(14): 811.37; b1+(3): 267.11; b1+(4): 396.15; b1+(5): 453.17; b1+(6): 582.22; b1+(7): 711.26; b1+(8): 826.28; b1+(9): 939.37; b1+(10): 1052.45; b1+(11): 1181.50; b1+(12): 1344.56; y1+(13): 1556.65; b2+(9): 470.19; b2+(10): 526.73","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AB4)","Search.ID":"AB","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KDELTLEGIK","Annotated.Sequence":"[K].KDELTLEGIK.[Q]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.516,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":573.32455,"Contaminant":"False","Aligned.m.z..Da.":573.32456,"MH...Da.":1145.64182,"Theo..MH...Da.":1145.64123,"DeltaM..ppm.":0.52,"Deltam.z..Da.":0.0003,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1119780.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":15.08332,"Ion.Inject.Time..ms.":22,"RT..min.":10.2659,"First.Scan":8648,"Last.Scan":8648,"Master.Scan.s.":8638,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_03.raw","File.ID":"F9","Quan.Info":"","Peptides.Matched":302,"XCorr":3.74,"X..Protein.Groups":1,"Percolator.q.Value":0.00034,"Percolator.PEP":1.295e-09,"Percolator.SVMScore":3.44,"Precursor.Area":801224.3125,"Apex.RT..min.":10.27,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AB4)","Search.ID":"AB","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"APQALMLAPTR","Annotated.Sequence":"[K].APQALmLAPTR.[E]","Modifications":"M6(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7032,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":592.82685,"Contaminant":"False","Aligned.m.z..Da.":592.82686,"MH...Da.":1184.64642,"Theo..MH...Da.":1184.6456,"DeltaM..ppm.":0.69,"Deltam.z..Da.":0.00041,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1067575.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":12.88016,"Ion.Inject.Time..ms.":22,"RT..min.":10.2036,"First.Scan":8569,"Last.Scan":8569,"Master.Scan.s.":8560,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_03.raw","File.ID":"F9","Quan.Info":"","Peptides.Matched":382,"XCorr":2.83,"X..Protein.Groups":1,"Percolator.q.Value":0.00034,"Percolator.PEP":5.434e-06,"Percolator.SVMScore":1.903,"Precursor.Area":819905.9375,"Apex.RT..min.":10.2,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AB4)","Search.ID":"AB","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].msEGITDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S2(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0789,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":731.31492,"Contaminant":"False","Aligned.m.z..Da.":731.3149,"MH...Da.":2191.93021,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":1.08,"Deltam.z..Da.":0.00079,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":222657.125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":39.25729,"Ion.Inject.Time..ms.":22,"RT..min.":15.7562,"First.Scan":15554,"Last.Scan":15554,"Master.Scan.s.":15546,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_03.raw","File.ID":"F9","Quan.Info":"","Peptides.Matched":2066,"XCorr":3.17,"X..Protein.Groups":1,"Percolator.q.Value":0.00034,"Percolator.PEP":7.835e-06,"Percolator.SVMScore":1.836,"Precursor.Area":414757.78125,"Apex.RT..min.":15.78,"ptmRS..Binomial.Peptide.Score":356.87,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(2): 112.04; b1+-H(3) O(4) P(3): 241.08; b1+-H(3) O(4) P(4): 298.10; b1+-H(3) O(4) P(5): 411.19; b1+-H(3) O(4) P(6): 512.24; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+-H(3) O(4) P(9): 869.39; b1+-H(3) O(4) P(10): 998.43","ptmRS..Best.Site.Probabilities":"S2(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 100.0; T(6): 0.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AB4)","Search.ID":"AB","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].msEGITDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S2(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1275,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1096.46713,"Contaminant":"False","Aligned.m.z..Da.":1096.4671,"MH...Da.":2191.92699,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":-0.39,"Deltam.z..Da.":-0.00043,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1978106.625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":15.7531,"First.Scan":15549,"Last.Scan":15549,"Master.Scan.s.":15546,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_03.raw","File.ID":"F9","Quan.Info":"","Peptides.Matched":1966,"XCorr":5.02,"X..Protein.Groups":1,"Percolator.q.Value":0.00034,"Percolator.PEP":3.171e-08,"Percolator.SVMScore":2.851,"Precursor.Area":4475345.5,"Apex.RT..min.":15.78,"ptmRS..Binomial.Peptide.Score":520.62,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(2): 112.04; b1+-H(3) O(4) P(3): 241.08; b1+-H(3) O(4) P(4): 298.10; b1+-H(3) O(4) P(5): 411.19; b1+-H(3) O(4) P(6): 512.24; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+-H(3) O(4) P(9): 869.39; b1+-H(3) O(4) P(10): 998.43; b1+-H(3) O(4) P(13): 1326.61; b1+(4): 396.08; y1+(14): 1683.77","ptmRS..Best.Site.Probabilities":"S2(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 100.0; T(6): 0.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AB4)","Search.ID":"AB","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FDDMELDENLLR","Annotated.Sequence":"[K].FDDMELDENLLR.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5097,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":755.34897,"Contaminant":"False","Aligned.m.z..Da.":755.34886,"MH...Da.":1509.69067,"Theo..MH...Da.":1509.68898,"DeltaM..ppm.":1.12,"Deltam.z..Da.":0.00084,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":381731.78125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":33.81253,"Ion.Inject.Time..ms.":22,"RT..min.":16.052,"First.Scan":15910,"Last.Scan":15910,"Master.Scan.s.":15905,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_03.raw","File.ID":"F9","Quan.Info":"","Peptides.Matched":1004,"XCorr":2.06,"X..Protein.Groups":1,"Percolator.q.Value":0.00034,"Percolator.PEP":0.0001,"Percolator.SVMScore":1.375,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AB4)","Search.ID":"AB","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0604,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40409,"Contaminant":"False","Aligned.m.z..Da.":967.40412,"MH...Da.":2900.19773,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":-0.55,"Deltam.z..Da.":-0.00053,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":305543.71875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":35.77915,"Ion.Inject.Time..ms.":22,"RT..min.":15.5514,"First.Scan":15325,"Last.Scan":15325,"Master.Scan.s.":15324,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_03.raw","File.ID":"F9","Quan.Info":"","Peptides.Matched":2423,"XCorr":4.14,"X..Protein.Groups":1,"Percolator.q.Value":0.00034,"Percolator.PEP":1.342e-08,"Percolator.SVMScore":3.009,"Precursor.Area":1598615.5,"Apex.RT..min.":15.36,"ptmRS..Binomial.Peptide.Score":348.54,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b2+-H(3) O(4) P(15): 821.83; b1+(12): 1344.56","ptmRS..Best.Site.Probabilities":"S13(Phospho): 99.99","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AB4)","Search.ID":"AB","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ILISTDLLAR","Annotated.Sequence":"[R].ILISTDLLAR.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.4462,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":557.84527,"Contaminant":"False","Aligned.m.z..Da.":557.84527,"MH...Da.":1114.68325,"Theo..MH...Da.":1114.68304,"DeltaM..ppm.":0.2,"Deltam.z..Da.":0.00011,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":518592.3125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":33.74769,"Ion.Inject.Time..ms.":22,"RT..min.":15.6275,"First.Scan":15398,"Last.Scan":15398,"Master.Scan.s.":15393,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_03.raw","File.ID":"F9","Quan.Info":"","Peptides.Matched":124,"XCorr":1.95,"X..Protein.Groups":1,"Percolator.q.Value":0.00034,"Percolator.PEP":0.00027,"Percolator.SVMScore":1.181,"Precursor.Area":591617.5,"Apex.RT..min.":15.6,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (X4)","Search.ID":"X","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ILISTDLLAR","Annotated.Sequence":"[R].ILISTDLLAR.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.4955,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":557.84532,"Contaminant":"False","Aligned.m.z..Da.":557.84529,"MH...Da.":1114.68336,"Theo..MH...Da.":1114.68304,"DeltaM..ppm.":0.29,"Deltam.z..Da.":0.00016,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":885934.875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":28.64263,"Ion.Inject.Time..ms.":22,"RT..min.":15.6048,"First.Scan":15072,"Last.Scan":15072,"Master.Scan.s.":15070,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_01.raw","File.ID":"F13","Quan.Info":"","Peptides.Matched":124,"XCorr":2.24,"X..Protein.Groups":1,"Percolator.q.Value":0.00035,"Percolator.PEP":0.00012,"Percolator.SVMScore":1.566,"Precursor.Area":671347.25,"Apex.RT..min.":15.6,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (X4)","Search.ID":"X","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"SSQIHIENESTEDILK","Annotated.Sequence":"[R].SSQIHIENEsTEDILK.[I]","Modifications":"S10(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.008,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":641.62974,"Contaminant":"False","Aligned.m.z..Da.":641.62974,"MH...Da.":1922.87469,"Theo..MH...Da.":1922.87429,"DeltaM..ppm.":0.21,"Deltam.z..Da.":0.00013,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":540334.1875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":47.64759,"Ion.Inject.Time..ms.":22,"RT..min.":11.2894,"First.Scan":9883,"Last.Scan":9883,"Master.Scan.s.":9875,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_01.raw","File.ID":"F13","Quan.Info":"","Peptides.Matched":1782,"XCorr":2.51,"X..Protein.Groups":1,"Percolator.q.Value":0.00035,"Percolator.PEP":0.00011,"Percolator.SVMScore":1.578,"Precursor.Area":336147.3125,"Apex.RT..min.":11.29,"ptmRS..Binomial.Peptide.Score":203.16,"ptmRS..Isoform.Confidence.Probability":0.008,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(7): 787.42; y1+-H(3) O(4) P(6): 700.39","ptmRS..Best.Site.Probabilities":"T11(Phospho): 99.24","ptmRS..Phospho.Site.Probabilities":"S(1): 0.0; S(2): 0.0; S(10): 0.8; T(11): 99.2"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (X4)","Search.ID":"X","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KDELTLEGIK","Annotated.Sequence":"[K].KDELTLEGIK.[Q]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.5966,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":382.55154,"Contaminant":"False","Aligned.m.z..Da.":382.55153,"MH...Da.":1145.64008,"Theo..MH...Da.":1145.64123,"DeltaM..ppm.":-1,"Deltam.z..Da.":-0.00038,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1388867,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":3.22685,"Ion.Inject.Time..ms.":22,"RT..min.":10.2466,"First.Scan":8546,"Last.Scan":8546,"Master.Scan.s.":8536,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_01.raw","File.ID":"F13","Quan.Info":"","Peptides.Matched":369,"XCorr":2.9,"X..Protein.Groups":1,"Percolator.q.Value":0.00035,"Percolator.PEP":0.00038,"Percolator.SVMScore":1.319,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (X4)","Search.ID":"X","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DIFSNDELLSDAYDAK","Annotated.Sequence":"[K].DIFSNDELLSDAYDAK.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.6394,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":605.94768,"Contaminant":"False","Aligned.m.z..Da.":605.9476,"MH...Da.":1815.82849,"Theo..MH...Da.":1815.82831,"DeltaM..ppm.":0.1,"Deltam.z..Da.":0.00006,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":214099.98438,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":27.79671,"Ion.Inject.Time..ms.":22,"RT..min.":17.0643,"First.Scan":16704,"Last.Scan":16704,"Master.Scan.s.":16698,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_01.raw","File.ID":"F13","Quan.Info":"","Peptides.Matched":1558,"XCorr":2.08,"X..Protein.Groups":1,"Percolator.q.Value":0.00035,"Percolator.PEP":0.00022,"Percolator.SVMScore":1.428,"Precursor.Area":323007.9375,"Apex.RT..min.":17.07,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (X4)","Search.ID":"X","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSSPLHRLSPTGK","Annotated.Sequence":"[R].mSsPLHRLsPTGK.[S]","Modifications":"M1(Oxidation); S3(Phospho); S9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.5132,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":529.56452,"Contaminant":"False","Aligned.m.z..Da.":529.56457,"MH...Da.":1586.679,"Theo..MH...Da.":1586.67977,"DeltaM..ppm.":-0.49,"Deltam.z..Da.":-0.00026,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3674253.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":10.87912,"Ion.Inject.Time..ms.":22,"RT..min.":7.7315,"First.Scan":5547,"Last.Scan":5547,"Master.Scan.s.":5546,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_01.raw","File.ID":"F13","Quan.Info":"","Peptides.Matched":979,"XCorr":3.41,"X..Protein.Groups":1,"Percolator.q.Value":0.00035,"Percolator.PEP":0.00023,"Percolator.SVMScore":1.424,"Precursor.Area":3406560.5,"Apex.RT..min.":7.74,"ptmRS..Binomial.Peptide.Score":265.23,"ptmRS..Isoform.Confidence.Probability":0.993,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(8): 877.50; y1+-H(3) O(4) P(7): 740.44; y2+-H(3) O(4) P(11): 627.82; y2+-H(3) O(4) P(10): 544.32; y2+-H(3) O(4) P(8): 439.25; y1+(4): 402.23","ptmRS..Best.Site.Probabilities":"S3(Phospho): 100; S9(Phospho): 99.33","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(3): 100.0; S(9): 99.3; T(11): 0.7"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (X4)","Search.ID":"X","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"IGNNELLHR","Annotated.Sequence":"[K].IGNNELLHR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q03558","Master.Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","Protein.Accessions":"Q03558","Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.5286,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":355.86505,"Contaminant":"False","Aligned.m.z..Da.":355.86508,"MH...Da.":1065.58061,"Theo..MH...Da.":1065.57997,"DeltaM..ppm.":0.61,"Deltam.z..Da.":0.00022,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1561498.875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":9.83502,"Ion.Inject.Time..ms.":22,"RT..min.":7.5764,"First.Scan":5330,"Last.Scan":5330,"Master.Scan.s.":5325,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_01.raw","File.ID":"F13","Quan.Info":"","Peptides.Matched":300,"XCorr":2.1,"X..Protein.Groups":1,"Percolator.q.Value":0.00035,"Percolator.PEP":0.00018,"Percolator.SVMScore":1.481,"Precursor.Area":916017.5,"Apex.RT..min.":7.58,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (X4)","Search.ID":"X","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].msEGITDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S2(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1542,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1096.46719,"Contaminant":"False","Aligned.m.z..Da.":1096.46707,"MH...Da.":2191.92712,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":-0.33,"Deltam.z..Da.":-0.00036,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":61233.38672,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":16.3035,"First.Scan":15834,"Last.Scan":15834,"Master.Scan.s.":15831,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_01.raw","File.ID":"F13","Quan.Info":"","Peptides.Matched":1941,"XCorr":2.27,"X..Protein.Groups":1,"Percolator.q.Value":0.00035,"Percolator.PEP":0.00014,"Percolator.SVMScore":1.523,"Precursor.Area":203461.96875,"Apex.RT..min.":16.35,"ptmRS..Binomial.Peptide.Score":421.91,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(2): 112.04; b1+-H(3) O(4) P(3): 241.08; b1+-H(3) O(4) P(4): 298.10; b1+-H(3) O(4) P(5): 411.19; b1+-H(3) O(4) P(6): 512.24; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+-H(3) O(4) P(9): 869.39; b1+(4): 396.08","ptmRS..Best.Site.Probabilities":"S2(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 100.0; T(6): 0.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AD4)","Search.ID":"AD","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LQETNPEEVPK","Annotated.Sequence":"[K].LQETNPEEVPK.[F]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5308,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":642.32773,"Contaminant":"False","Aligned.m.z..Da.":642.32777,"MH...Da.":1283.64819,"Theo..MH...Da.":1283.64777,"DeltaM..ppm.":0.32,"Deltam.z..Da.":0.00021,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1585764.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":11.85795,"Ion.Inject.Time..ms.":22,"RT..min.":7.6577,"First.Scan":5419,"Last.Scan":5419,"Master.Scan.s.":5414,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_01.raw","File.ID":"F7","Quan.Info":"","Peptides.Matched":558,"XCorr":2.6,"X..Protein.Groups":1,"Percolator.q.Value":0.00035,"Percolator.PEP":0.00049,"Percolator.SVMScore":1.18,"Precursor.Area":1907243.875,"Apex.RT..min.":7.67,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AD4)","Search.ID":"AD","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSSPLHRLSPTGK","Annotated.Sequence":"[R].mSsPLHRLsPTGK.[S]","Modifications":"M1(Oxidation); S3(Phospho); S9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.4872,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":529.5647,"Contaminant":"False","Aligned.m.z..Da.":529.56473,"MH...Da.":1586.67954,"Theo..MH...Da.":1586.67977,"DeltaM..ppm.":-0.15,"Deltam.z..Da.":-0.00008,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1985970.125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":55.91613,"Ion.Inject.Time..ms.":22,"RT..min.":7.7374,"First.Scan":5537,"Last.Scan":5537,"Master.Scan.s.":5531,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_01.raw","File.ID":"F7","Quan.Info":"","Peptides.Matched":988,"XCorr":3.12,"X..Protein.Groups":1,"Percolator.q.Value":0.00035,"Percolator.PEP":0.00053,"Percolator.SVMScore":1.161,"Precursor.Area":1502574.625,"Apex.RT..min.":7.74,"ptmRS..Binomial.Peptide.Score":204.92,"ptmRS..Isoform.Confidence.Probability":0.989,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(8): 877.50; y1+-H(3) O(4) P(7): 740.44; y2+-H(3) O(4) P(11): 627.82; y2+-H(3) O(4) P(10): 544.32; y2+-H(3) O(4) P(8): 439.25; y1+(4): 402.23","ptmRS..Best.Site.Probabilities":"S3(Phospho): 100; S9(Phospho): 98.92","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(3): 100.0; S(9): 98.9; T(11): 1.1"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AD4)","Search.ID":"AD","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].msEGITDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S2(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0565,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":731.31413,"Contaminant":"False","Aligned.m.z..Da.":731.314,"MH...Da.":2191.92784,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":0,"Deltam.z..Da.":0,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":157740.17188,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":25.88204,"Ion.Inject.Time..ms.":22,"RT..min.":15.7721,"First.Scan":14562,"Last.Scan":14562,"Master.Scan.s.":14552,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_01.raw","File.ID":"F7","Quan.Info":"","Peptides.Matched":1961,"XCorr":2.83,"X..Protein.Groups":1,"Percolator.q.Value":0.00035,"Percolator.PEP":0.00028,"Percolator.SVMScore":1.328,"Precursor.Area":367118.1875,"Apex.RT..min.":15.8,"ptmRS..Binomial.Peptide.Score":329.2,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(2): 112.04; b1+-H(3) O(4) P(3): 241.08; b1+-H(3) O(4) P(4): 298.10; b1+-H(3) O(4) P(5): 411.19; b1+-H(3) O(4) P(6): 512.24; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+-H(3) O(4) P(9): 869.39","ptmRS..Best.Site.Probabilities":"S2(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 100.0; T(6): 0.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AD4)","Search.ID":"AD","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].msEGITDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S2(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":3,"DeltaScore":0.1573,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1096.46775,"Contaminant":"False","Aligned.m.z..Da.":1096.46755,"MH...Da.":2191.92822,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":0.17,"Deltam.z..Da.":0.00019,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":755432.125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":36.9553,"Ion.Inject.Time..ms.":22,"RT..min.":15.7591,"First.Scan":14545,"Last.Scan":14545,"Master.Scan.s.":14544,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_01.raw","File.ID":"F7","Quan.Info":"","Peptides.Matched":2009,"XCorr":2.67,"X..Protein.Groups":1,"Percolator.q.Value":0.00035,"Percolator.PEP":0.00076,"Percolator.SVMScore":1.069,"Precursor.Area":2129410.5,"Apex.RT..min.":15.8,"ptmRS..Binomial.Peptide.Score":219.27,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(2): 112.04; b1+-H(3) O(4) P(3): 241.08; b1+-H(3) O(4) P(4): 298.10; b1+-H(3) O(4) P(5): 411.19; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+-H(3) O(4) P(9): 869.39","ptmRS..Best.Site.Probabilities":"S2(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 100.0; T(6): 0.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AD4)","Search.ID":"AD","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KDELTLEGIK","Annotated.Sequence":"[K].KDELTLEGIK.[Q]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.5154,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":382.55169,"Contaminant":"False","Aligned.m.z..Da.":382.55166,"MH...Da.":1145.64052,"Theo..MH...Da.":1145.64123,"DeltaM..ppm.":-0.62,"Deltam.z..Da.":-0.00024,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":985377.625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":33.45793,"Ion.Inject.Time..ms.":22,"RT..min.":10.2658,"First.Scan":8542,"Last.Scan":8542,"Master.Scan.s.":8534,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_01.raw","File.ID":"F7","Quan.Info":"","Peptides.Matched":314,"XCorr":2.6,"X..Protein.Groups":1,"Percolator.q.Value":0.00035,"Percolator.PEP":0.00045,"Percolator.SVMScore":1.205,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AD4)","Search.ID":"AD","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"APQALMLAPTR","Annotated.Sequence":"[K].APQALMLAPTR.[E]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":3,"DeltaScore":0.3094,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":584.82703,"Contaminant":"False","Aligned.m.z..Da.":584.82701,"MH...Da.":1168.64678,"Theo..MH...Da.":1168.65069,"DeltaM..ppm.":-3.35,"Deltam.z..Da.":-0.00196,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":75256.79688,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":96.03938,"Ion.Inject.Time..ms.":22,"RT..min.":11.6188,"First.Scan":10286,"Last.Scan":10286,"Master.Scan.s.":10282,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_01.raw","File.ID":"F7","Quan.Info":"","Peptides.Matched":221,"XCorr":1.39,"X..Protein.Groups":1,"Percolator.q.Value":0.00035,"Percolator.PEP":0.00055,"Percolator.SVMScore":1.149,"Precursor.Area":206288.70312,"Apex.RT..min.":11.64,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (J4)","Search.ID":"J","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LQETNPEEVPK","Annotated.Sequence":"[K].LQETNPEEVPK.[F]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5487,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":642.32805,"Contaminant":"False","Aligned.m.z..Da.":642.32804,"MH...Da.":1283.64884,"Theo..MH...Da.":1283.64777,"DeltaM..ppm.":0.83,"Deltam.z..Da.":0.00053,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3617565.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":9.91416,"Ion.Inject.Time..ms.":22,"RT..min.":7.6319,"First.Scan":5432,"Last.Scan":5432,"Master.Scan.s.":5427,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_03.raw","File.ID":"F27","Quan.Info":"","Peptides.Matched":559,"XCorr":2.77,"X..Protein.Groups":1,"Percolator.q.Value":0.00035,"Percolator.PEP":0.00006,"Percolator.SVMScore":2.022,"Precursor.Area":9215759,"Apex.RT..min.":7.65,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (J4)","Search.ID":"J","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"HSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[R].HSDSySENETNHTNVPISSTGGTNNK.[T]","Modifications":"Y5(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0136,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":957.40008,"Contaminant":"False","Aligned.m.z..Da.":957.40007,"MH...Da.":2870.1857,"Theo..MH...Da.":2870.18608,"DeltaM..ppm.":-0.13,"Deltam.z..Da.":-0.00013,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2401247,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":7.6503,"First.Scan":5459,"Last.Scan":5459,"Master.Scan.s.":5453,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_03.raw","File.ID":"F27","Quan.Info":"","Peptides.Matched":2478,"XCorr":5.87,"X..Protein.Groups":1,"Percolator.q.Value":0.00035,"Percolator.PEP":1.633e-09,"Percolator.SVMScore":5.028,"Precursor.Area":12232695,"Apex.RT..min.":7.66,"ptmRS..Binomial.Peptide.Score":315.71,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(4): 409.15; b1+-H(3) O(4) P(5): 572.21; b1+-H(3) O(4) P(6): 659.24; b1+-H(3) O(4) P(11): 1246.46; b1+-H(3) O(4) P(12): 1383.52; b1+-H(3) O(4) P(15): 1697.68; b2+-H(3) O(4) P(12): 692.26; b2+-H(3) O(4) P(13): 742.79; b2+-H(3) O(4) P(14): 799.81; b2+-H(3) O(4) P(15): 849.34; y2+-H(3) O(4) P(25): 1318.08; y2+-H(3) O(4) P(24): 1274.56; y2+-H(3) O(4) P(23): 1217.05","ptmRS..Best.Site.Probabilities":"S4(Phospho): 99.41","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(4): 99.4; Y(5): 0.0; S(6): 0.6; T(10): 0.0; T(13): 0.0; S(18): 0.0; S(19): 0.0; T(20): 0.0; T(23): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (J4)","Search.ID":"J","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"HSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[R].HSDSySENETNHTNVPISSTGGTNNK.[T]","Modifications":"Y5(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":0,"Charge":4,"Original.Precursor.Charge":4,"DeltaScore":0.0051,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":718.30173,"Contaminant":"False","Aligned.m.z..Da.":718.30172,"MH...Da.":2870.18512,"Theo..MH...Da.":2870.18608,"DeltaM..ppm.":-0.33,"Deltam.z..Da.":-0.00024,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":6392720,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":20.737,"RT..min.":7.658,"First.Scan":5470,"Last.Scan":5470,"Master.Scan.s.":5466,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_03.raw","File.ID":"F27","Quan.Info":"","Peptides.Matched":2458,"XCorr":5.88,"X..Protein.Groups":1,"Percolator.q.Value":0.00035,"Percolator.PEP":4.48e-08,"Percolator.SVMScore":4.083,"Precursor.Area":5850293,"Apex.RT..min.":7.66,"ptmRS..Binomial.Peptide.Score":282.25,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(9): 1031.37; b1+-H(3) O(4) P(10): 1132.42; b1+-H(3) O(4) P(11): 1246.46; b1+-H(3) O(4) P(12): 1383.52; b1+-H(3) O(4) P(15): 1697.68; b2+-H(3) O(4) P(7): 394.65; b2+-H(3) O(4) P(8): 451.67; b2+-H(3) O(4) P(13): 742.79; b2+-H(3) O(4) P(14): 799.81; b2+-H(3) O(4) P(15): 849.34; b2+-H(3) O(4) P(17): 954.41; b1+(4): 427.16","ptmRS..Best.Site.Probabilities":"S6(Phospho): 99.39","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(4): 0.6; Y(5): 0.0; S(6): 99.4; T(10): 0.0; T(13): 0.0; S(18): 0.0; S(19): 0.0; T(20): 0.0; T(23): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (J4)","Search.ID":"J","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"IGNNELLHR","Annotated.Sequence":"[K].IGNNELLHR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q03558","Master.Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","Protein.Accessions":"Q03558","Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.5348,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":355.86501,"Contaminant":"False","Aligned.m.z..Da.":355.865,"MH...Da.":1065.58047,"Theo..MH...Da.":1065.57997,"DeltaM..ppm.":0.48,"Deltam.z..Da.":0.00017,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2094192.875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":15.59835,"Ion.Inject.Time..ms.":22,"RT..min.":7.5799,"First.Scan":5355,"Last.Scan":5355,"Master.Scan.s.":5349,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_03.raw","File.ID":"F27","Quan.Info":"","Peptides.Matched":300,"XCorr":2.3,"X..Protein.Groups":1,"Percolator.q.Value":0.00035,"Percolator.PEP":0.00013,"Percolator.SVMScore":1.793,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (J4)","Search.ID":"J","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"AAEAGETGAATSATEGDNNNNTAAGDKK","Annotated.Sequence":"[K].AAEAGETGAATsATEGDNNNNTAAGDKK.[G]","Modifications":"S12(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0102,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":906.04928,"Contaminant":"False","Aligned.m.z..Da.":906.04918,"MH...Da.":2716.1333,"Theo..MH...Da.":2716.13298,"DeltaM..ppm.":0.12,"Deltam.z..Da.":0.00011,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":6180360.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":2.38623,"Ion.Inject.Time..ms.":21.879,"RT..min.":5.0217,"First.Scan":1790,"Last.Scan":1790,"Master.Scan.s.":1787,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_03.raw","File.ID":"F27","Quan.Info":"","Peptides.Matched":2525,"XCorr":6.85,"X..Protein.Groups":1,"Percolator.q.Value":0.00035,"Percolator.PEP":1.013e-12,"Percolator.SVMScore":7.135,"Precursor.Area":4024744.75,"Apex.RT..min.":5.02,"ptmRS..Binomial.Peptide.Score":562.2,"ptmRS..Isoform.Confidence.Probability":0.993,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(15): 1300.57; y1+-H(3) O(4) P(18): 1789.79; y2+-H(3) O(4) P(25): 1174.02; y2+-H(3) O(4) P(24): 1138.50; y2+-H(3) O(4) P(22): 1045.47; y2+-H(3) O(4) P(18): 895.40; y2+-H(3) O(4) P(17): 844.88","ptmRS..Best.Site.Probabilities":"S12(Phospho): 99.29","ptmRS..Phospho.Site.Probabilities":"T(7): 0.0; T(11): 0.7; S(12): 99.3; T(14): 0.0; T(22): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (J4)","Search.ID":"J","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"AAEAGETGAATSATEGDNNNNTAAGDKK","Annotated.Sequence":"[K].AAEAGETGAATSATEGDNNNNTAAGDKK.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.7905,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":879.3931,"Contaminant":"False","Aligned.m.z..Da.":879.39299,"MH...Da.":2636.16474,"Theo..MH...Da.":2636.16665,"DeltaM..ppm.":-0.72,"Deltam.z..Da.":-0.00064,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3549524,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":1.93693,"Ion.Inject.Time..ms.":22,"RT..min.":4.933,"First.Scan":1708,"Last.Scan":1708,"Master.Scan.s.":1696,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_03.raw","File.ID":"F27","Quan.Info":"","Peptides.Matched":3709,"XCorr":8.02,"X..Protein.Groups":1,"Percolator.q.Value":0.00035,"Percolator.PEP":3.989e-15,"Percolator.SVMScore":8.715,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (J4)","Search.ID":"J","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KFQDDTLNR","Annotated.Sequence":"[K].KFQDDtLNR.[V]","Modifications":"T6(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6823,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":608.77196,"Contaminant":"False","Aligned.m.z..Da.":608.7719,"MH...Da.":1216.53665,"Theo..MH...Da.":1216.53579,"DeltaM..ppm.":0.7,"Deltam.z..Da.":0.00043,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2032785.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":25.26912,"Ion.Inject.Time..ms.":22,"RT..min.":6.2227,"First.Scan":3186,"Last.Scan":3186,"Master.Scan.s.":3178,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_03.raw","File.ID":"F27","Quan.Info":"","Peptides.Matched":615,"XCorr":2.99,"X..Protein.Groups":1,"Percolator.q.Value":0.00035,"Percolator.PEP":0.00023,"Percolator.SVMScore":1.63,"Precursor.Area":3145892,"Apex.RT..min.":6.23,"ptmRS..Binomial.Peptide.Score":336.03,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"T6(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"T(6): 100.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (J4)","Search.ID":"J","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"MGSINNNFNTNNNSNTDLDRDWK","Annotated.Sequence":"[-].mGSINNNFNTNNNsNTDLDRDWK.[T]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S14(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P39517","Master.Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","Protein.Accessions":"P39517","Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.011,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1338.06031,"Contaminant":"False","Aligned.m.z..Da.":1338.06071,"MH...Da.":2675.11335,"Theo..MH...Da.":2675.11179,"DeltaM..ppm.":0.58,"Deltam.z..Da.":0.00078,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":957760.3125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":13.5731,"First.Scan":13033,"Last.Scan":13033,"Master.Scan.s.":13032,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_03.raw","File.ID":"F27","Quan.Info":"","Peptides.Matched":2261,"XCorr":4.56,"X..Protein.Groups":1,"Percolator.q.Value":0.00035,"Percolator.PEP":1.099e-06,"Percolator.SVMScore":3.17,"Precursor.Area":868758.5625,"Apex.RT..min.":13.58,"ptmRS..Binomial.Peptide.Score":513.67,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(17): 2049.90; y1+-H(3) O(4) P(14): 1674.75; y1+-H(3) O(4) P(13): 1573.70; y1+-H(3) O(4) P(12): 1459.66; y1+-H(3) O(4) P(10): 1231.57; b2+-H(3) O(4) P(19): 987.41; y2+-H(3) O(4) P(19): 1139.50; y1+(9): 1162.55; y1+(8): 1048.51","ptmRS..Best.Site.Probabilities":"S14(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(3): 0.0; T(10): 0.0; S(14): 100.0; T(16): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (J4)","Search.ID":"J","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FDDMELDENLLR","Annotated.Sequence":"[K].FDDmELDENLLR.[G]","Modifications":"M4(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5591,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":763.34486,"Contaminant":"False","Aligned.m.z..Da.":763.34503,"MH...Da.":1525.68245,"Theo..MH...Da.":1525.6839,"DeltaM..ppm.":-0.95,"Deltam.z..Da.":-0.00073,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1648252.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":70.79895,"Ion.Inject.Time..ms.":22,"RT..min.":14.4757,"First.Scan":14181,"Last.Scan":14181,"Master.Scan.s.":14176,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_03.raw","File.ID":"F27","Quan.Info":"","Peptides.Matched":1169,"XCorr":2.79,"X..Protein.Groups":1,"Percolator.q.Value":0.00035,"Percolator.PEP":0.00001,"Percolator.SVMScore":2.438,"Precursor.Area":1631650.625,"Apex.RT..min.":14.49,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (J4)","Search.ID":"J","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].mSEGITDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.668,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1056.48087,"Contaminant":"False","Aligned.m.z..Da.":1056.48106,"MH...Da.":2111.95447,"Theo..MH...Da.":2111.96151,"DeltaM..ppm.":-3.33,"Deltam.z..Da.":-0.00352,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":349466.03125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":16.23282,"Ion.Inject.Time..ms.":22,"RT..min.":14.6533,"First.Scan":14409,"Last.Scan":14409,"Master.Scan.s.":14397,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_03.raw","File.ID":"F27","Quan.Info":"","Peptides.Matched":2200,"XCorr":5.15,"X..Protein.Groups":1,"Percolator.q.Value":0.00035,"Percolator.PEP":2.708e-08,"Percolator.SVMScore":4.227,"Precursor.Area":1239719.25,"Apex.RT..min.":14.66,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (J4)","Search.ID":"J","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DFLLKPELSR","Annotated.Sequence":"[K].DFLLKPELSR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.6057,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":406.56773,"Contaminant":"False","Aligned.m.z..Da.":406.56782,"MH...Da.":1217.68866,"Theo..MH...Da.":1217.68885,"DeltaM..ppm.":-0.16,"Deltam.z..Da.":-0.00006,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":532858.625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":2.3646,"Ion.Inject.Time..ms.":22,"RT..min.":13.1596,"First.Scan":12508,"Last.Scan":12508,"Master.Scan.s.":12499,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_03.raw","File.ID":"F27","Quan.Info":"","Peptides.Matched":265,"XCorr":2.46,"X..Protein.Groups":1,"Percolator.q.Value":0.00035,"Percolator.PEP":0.00007,"Percolator.SVMScore":1.987,"Precursor.Area":486944.09375,"Apex.RT..min.":13.15,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (J4)","Search.ID":"J","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"AIMPIIEGHDVLAQAQSGTGK","Annotated.Sequence":"[R].AImPIIEGHDVLAQAQSGTGK.[T]","Modifications":"M3(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.7476,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":718.0398,"Contaminant":"False","Aligned.m.z..Da.":718.03988,"MH...Da.":2152.10484,"Theo..MH...Da.":2152.10668,"DeltaM..ppm.":-0.85,"Deltam.z..Da.":-0.00061,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":825173.4375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":8.63024,"Ion.Inject.Time..ms.":22,"RT..min.":12.7638,"First.Scan":11991,"Last.Scan":11991,"Master.Scan.s.":11979,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_03.raw","File.ID":"F27","Quan.Info":"","Peptides.Matched":996,"XCorr":5.19,"X..Protein.Groups":1,"Percolator.q.Value":0.00035,"Percolator.PEP":4.983e-09,"Percolator.SVMScore":4.71,"Precursor.Area":1543087.125,"Apex.RT..min.":12.78,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (J4)","Search.ID":"J","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DIFSNDELLSDAYDAK","Annotated.Sequence":"[K].DIFSNDELLSDAYDAK.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6976,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":908.42058,"Contaminant":"False","Aligned.m.z..Da.":908.42021,"MH...Da.":1815.83389,"Theo..MH...Da.":1815.82831,"DeltaM..ppm.":3.07,"Deltam.z..Da.":0.00279,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":755480,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":14.61745,"Ion.Inject.Time..ms.":22,"RT..min.":17.0709,"First.Scan":17480,"Last.Scan":17480,"Master.Scan.s.":17477,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_03.raw","File.ID":"F27","Quan.Info":"","Peptides.Matched":1791,"XCorr":3.77,"X..Protein.Groups":1,"Percolator.q.Value":0.00035,"Percolator.PEP":6.471e-08,"Percolator.SVMScore":3.978,"Precursor.Area":3461139,"Apex.RT..min.":17.1,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (J4)","Search.ID":"J","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.4096,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40421,"Contaminant":"False","Aligned.m.z..Da.":967.40403,"MH...Da.":2900.19808,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":-0.43,"Deltam.z..Da.":-0.00042,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":351638.375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":17.88046,"Ion.Inject.Time..ms.":22,"RT..min.":16.4177,"First.Scan":16650,"Last.Scan":16650,"Master.Scan.s.":16645,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_03.raw","File.ID":"F27","Quan.Info":"","Peptides.Matched":2443,"XCorr":3.76,"X..Protein.Groups":1,"Percolator.q.Value":0.00035,"Percolator.PEP":0.00002,"Percolator.SVMScore":2.292,"Precursor.Area":423718.65625,"Apex.RT..min.":16.43,"ptmRS..Binomial.Peptide.Score":306.95,"ptmRS..Isoform.Confidence.Probability":0.992,"ptmRS..Isoform.Group.Report":"b1+(12): 1344.56","ptmRS..Best.Site.Probabilities":"S13(Phospho): 99.22","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.8; S(13): 99.2; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (J4)","Search.ID":"J","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MFILDEADEMLSSGFK","Annotated.Sequence":"[K].mFILDEADEmLSSGFK.[E]","Modifications":"M1(Oxidation); M10(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7382,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":932.91903,"Contaminant":"False","Aligned.m.z..Da.":932.91879,"MH...Da.":1864.83078,"Theo..MH...Da.":1864.83433,"DeltaM..ppm.":-1.9,"Deltam.z..Da.":-0.00178,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":580207.125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":16.6843,"First.Scan":16987,"Last.Scan":16987,"Master.Scan.s.":16983,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_03.raw","File.ID":"F27","Quan.Info":"","Peptides.Matched":1829,"XCorr":3.82,"X..Protein.Groups":1,"Percolator.q.Value":0.00035,"Percolator.PEP":6.505e-08,"Percolator.SVMScore":3.977,"Precursor.Area":697506.25,"Apex.RT..min.":16.7,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (J4)","Search.ID":"J","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MFILDEADEMLSSGFK","Annotated.Sequence":"[K].mFILDEADEmLSSGFK.[E]","Modifications":"M1(Oxidation); M10(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.6277,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":622.28291,"Contaminant":"False","Aligned.m.z..Da.":622.28275,"MH...Da.":1864.8342,"Theo..MH...Da.":1864.83433,"DeltaM..ppm.":-0.07,"Deltam.z..Da.":-0.00004,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":473646.53125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":16.7098,"First.Scan":17021,"Last.Scan":17021,"Master.Scan.s.":17009,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_03.raw","File.ID":"F27","Quan.Info":"","Peptides.Matched":1838,"XCorr":2.74,"X..Protein.Groups":1,"Percolator.q.Value":0.00035,"Percolator.PEP":0.00018,"Percolator.SVMScore":1.695,"Precursor.Area":292503.09375,"Apex.RT..min.":16.7,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (J4)","Search.ID":"J","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":4,"DeltaScore":0.0613,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40888,"Contaminant":"False","Aligned.m.z..Da.":967.4089,"MH...Da.":2900.21209,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":4.4,"Deltam.z..Da.":0.00425,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2778951,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":76.6156,"Ion.Inject.Time..ms.":22,"RT..min.":15.2173,"First.Scan":15128,"Last.Scan":15128,"Master.Scan.s.":15125,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_03.raw","File.ID":"F27","Quan.Info":"","Peptides.Matched":2665,"XCorr":4.08,"X..Protein.Groups":1,"Percolator.q.Value":0.00035,"Percolator.PEP":0.00001,"Percolator.SVMScore":2.475,"ptmRS..Binomial.Peptide.Score":390.65,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(13): 1413.58; b1+-H(3) O(4) P(16): 1771.69; y1+-H(3) O(4) P(14): 1621.73; b2+-H(3) O(4) P(18): 1014.90; y2+-H(3) O(4) P(14): 811.37; b1+(12): 1344.56","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (J4)","Search.ID":"J","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":2,"DeltaScore":0.0393,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40875,"Contaminant":"False","Aligned.m.z..Da.":967.40878,"MH...Da.":2900.21171,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":4.27,"Deltam.z..Da.":0.00413,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":4428164,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":39.37547,"Ion.Inject.Time..ms.":22,"RT..min.":15.2008,"First.Scan":15109,"Last.Scan":15109,"Master.Scan.s.":15099,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_03.raw","File.ID":"F27","Quan.Info":"","Peptides.Matched":2676,"XCorr":4.84,"X..Protein.Groups":1,"Percolator.q.Value":0.00035,"Percolator.PEP":7.815e-06,"Percolator.SVMScore":2.611,"ptmRS..Binomial.Peptide.Score":426.57,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(13): 1413.58; b1+-H(3) O(4) P(14): 1528.61; y1+-H(3) O(4) P(15): 1750.78; y1+-H(3) O(4) P(14): 1621.73; y1+-H(3) O(4) P(13): 1458.67; b1+(12): 1344.56","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (J4)","Search.ID":"J","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":5,"DeltaScore":0.0864,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40429,"Contaminant":"False","Aligned.m.z..Da.":967.40433,"MH...Da.":2900.19831,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":-0.35,"Deltam.z..Da.":-0.00034,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":8947954,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":9.39206,"Ion.Inject.Time..ms.":12.589,"RT..min.":15.1552,"First.Scan":15050,"Last.Scan":15050,"Master.Scan.s.":15047,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_03.raw","File.ID":"F27","Quan.Info":"","Peptides.Matched":2428,"XCorr":6.48,"X..Protein.Groups":1,"Percolator.q.Value":0.00035,"Percolator.PEP":4.93e-11,"Percolator.SVMScore":6.027,"Precursor.Area":49675996,"Apex.RT..min.":15.09,"ptmRS..Binomial.Peptide.Score":786.89,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(13): 1413.58; b1+-H(3) O(4) P(14): 1528.61; b1+-H(3) O(4) P(15): 1642.65; b1+-H(3) O(4) P(16): 1771.69; y1+-H(3) O(4) P(15): 1750.78; y1+-H(3) O(4) P(14): 1621.73; y1+-H(3) O(4) P(13): 1458.67; b2+-H(3) O(4) P(16): 886.35; b2+-H(3) O(4) P(17): 950.38; b2+-H(3) O(4) P(18): 1014.90; y2+-H(3) O(4) P(14): 811.37; y2+-H(3) O(4) P(13): 729.84; b1+(12): 1344.56; y1+(13): 1556.65","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (J4)","Search.ID":"J","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0548,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.4061,"Contaminant":"False","Aligned.m.z..Da.":967.40609,"MH...Da.":2900.20376,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":1.53,"Deltam.z..Da.":0.00148,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2101269.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":7.2009,"Ion.Inject.Time..ms.":22,"RT..min.":15.3785,"First.Scan":15334,"Last.Scan":15334,"Master.Scan.s.":15333,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_03.raw","File.ID":"F27","Quan.Info":"","Peptides.Matched":2579,"XCorr":5.11,"X..Protein.Groups":1,"Percolator.q.Value":0.00035,"Percolator.PEP":8.306e-07,"Percolator.SVMScore":3.25,"Precursor.Area":49675996,"Apex.RT..min.":15.09,"ptmRS..Binomial.Peptide.Score":410.01,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(13): 1413.58; b1+-H(3) O(4) P(14): 1528.61; b1+-H(3) O(4) P(15): 1642.65; b1+-H(3) O(4) P(16): 1771.69; b1+(12): 1344.56","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (J4)","Search.ID":"J","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FFISNPDLVDRLEK","Annotated.Sequence":"[R].FFISNPDLVDRLEK.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q03558","Master.Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","Protein.Accessions":"Q03558","Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.7843,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":564.96994,"Contaminant":"False","Aligned.m.z..Da.":564.96994,"MH...Da.":1692.89529,"Theo..MH...Da.":1692.89555,"DeltaM..ppm.":-0.15,"Deltam.z..Da.":-0.00009,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":746749.4375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":49.01893,"Ion.Inject.Time..ms.":22,"RT..min.":15.3409,"First.Scan":15287,"Last.Scan":15287,"Master.Scan.s.":15281,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_03.raw","File.ID":"F27","Quan.Info":"","Peptides.Matched":554,"XCorr":3.06,"X..Protein.Groups":1,"Percolator.q.Value":0.00035,"Percolator.PEP":5.71e-07,"Percolator.SVMScore":3.357,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (J4)","Search.ID":"J","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.3629,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40691,"Contaminant":"False","Aligned.m.z..Da.":967.40703,"MH...Da.":2900.20619,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":2.37,"Deltam.z..Da.":0.00229,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":416569.59375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":14.8745,"First.Scan":14692,"Last.Scan":14692,"Master.Scan.s.":14683,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_03.raw","File.ID":"F27","Quan.Info":"","Peptides.Matched":2616,"XCorr":3.83,"X..Protein.Groups":1,"Percolator.q.Value":0.00035,"Percolator.PEP":0.00002,"Percolator.SVMScore":2.402,"Precursor.Area":801343.3125,"Apex.RT..min.":14.84,"ptmRS..Binomial.Peptide.Score":331.77,"ptmRS..Isoform.Confidence.Probability":0.992,"ptmRS..Isoform.Group.Report":"b2+-H(3) O(4) P(17): 950.38","ptmRS..Best.Site.Probabilities":"S13(Phospho): 99.15","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.9; S(13): 99.2; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (J4)","Search.ID":"J","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FTVSAIYSDLPQQER","Annotated.Sequence":"[K].FTVSAIYSDLPQQER.[D]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6953,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":877.44177,"Contaminant":"False","Aligned.m.z..Da.":877.44183,"MH...Da.":1753.87626,"Theo..MH...Da.":1753.87554,"DeltaM..ppm.":0.41,"Deltam.z..Da.":0.00036,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":732325.1875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":7.36717,"Ion.Inject.Time..ms.":22,"RT..min.":15.0259,"First.Scan":14885,"Last.Scan":14885,"Master.Scan.s.":14878,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_03.raw","File.ID":"F27","Quan.Info":"","Peptides.Matched":1082,"XCorr":3.84,"X..Protein.Groups":1,"Percolator.q.Value":0.00035,"Percolator.PEP":2.138e-08,"Percolator.SVMScore":4.294,"Precursor.Area":1171758.125,"Apex.RT..min.":15.04,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (J4)","Search.ID":"J","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"GVFGYGFEEPSAIQQR","Annotated.Sequence":"[R].GVFGYGFEEPSAIQQR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7179,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":892.93296,"Contaminant":"False","Aligned.m.z..Da.":892.93303,"MH...Da.":1784.85864,"Theo..MH...Da.":1784.86022,"DeltaM..ppm.":-0.89,"Deltam.z..Da.":-0.00079,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":446669.6875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":15.63441,"Ion.Inject.Time..ms.":22,"RT..min.":15.0164,"First.Scan":14873,"Last.Scan":14873,"Master.Scan.s.":14865,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_03.raw","File.ID":"F27","Quan.Info":"","Peptides.Matched":1270,"XCorr":3.58,"X..Protein.Groups":1,"Percolator.q.Value":0.00035,"Percolator.PEP":4.049e-07,"Percolator.SVMScore":3.455,"Precursor.Area":1662576.5,"Apex.RT..min.":15.04,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (J4)","Search.ID":"J","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].mSEGITDIEEsQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S11(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1279,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1096.46976,"Contaminant":"False","Aligned.m.z..Da.":1096.46985,"MH...Da.":2191.93225,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":2.01,"Deltam.z..Da.":0.0022,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":496173.46875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":17.25567,"Ion.Inject.Time..ms.":22,"RT..min.":15.0075,"First.Scan":14862,"Last.Scan":14862,"Master.Scan.s.":14852,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_03.raw","File.ID":"F27","Quan.Info":"","Peptides.Matched":2160,"XCorr":3.05,"X..Protein.Groups":1,"Percolator.q.Value":0.00035,"Percolator.PEP":0.00005,"Percolator.SVMScore":2.09,"Precursor.Area":306146.0625,"Apex.RT..min.":15,"ptmRS..Binomial.Peptide.Score":348.83,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(13): 1564.71; y1+-H(3) O(4) P(12): 1449.69; y1+-H(3) O(4) P(11): 1336.60; y1+-H(3) O(4) P(10): 1207.56; y1+-H(3) O(4) P(9): 1078.52; y1+(8): 1009.49; y1+(7): 881.44; y1+(6): 768.35; y1+(5): 640.29","ptmRS..Best.Site.Probabilities":"S11(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; T(6): 0.0; S(11): 100.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (J4)","Search.ID":"J","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].msEGITDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S2(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1356,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1096.46916,"Contaminant":"False","Aligned.m.z..Da.":1096.46902,"MH...Da.":2191.93105,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":1.46,"Deltam.z..Da.":0.0016,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":524476.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":16.0611,"First.Scan":16202,"Last.Scan":16202,"Master.Scan.s.":16191,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_03.raw","File.ID":"F27","Quan.Info":"","Peptides.Matched":2083,"XCorr":3.54,"X..Protein.Groups":1,"Percolator.q.Value":0.00035,"Percolator.PEP":6.568e-07,"Percolator.SVMScore":3.317,"Precursor.Area":23399554,"Apex.RT..min.":15.78,"ptmRS..Binomial.Peptide.Score":468.9,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(2): 112.04; b1+-H(3) O(4) P(3): 241.08; b1+-H(3) O(4) P(4): 298.10; b1+-H(3) O(4) P(5): 411.19; b1+-H(3) O(4) P(6): 512.24; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+-H(3) O(4) P(9): 869.39; b1+-H(3) O(4) P(10): 998.43; y1+(14): 1683.77","ptmRS..Best.Site.Probabilities":"S2(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 100.0; T(6): 0.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (J4)","Search.ID":"J","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FDDMELDENLLR","Annotated.Sequence":"[K].FDDMELDENLLR.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5882,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":755.34647,"Contaminant":"False","Aligned.m.z..Da.":755.34637,"MH...Da.":1509.68566,"Theo..MH...Da.":1509.68898,"DeltaM..ppm.":-2.2,"Deltam.z..Da.":-0.00166,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":993686.375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":8.15278,"Ion.Inject.Time..ms.":22,"RT..min.":16.0366,"First.Scan":16170,"Last.Scan":16170,"Master.Scan.s.":16165,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_03.raw","File.ID":"F27","Quan.Info":"","Peptides.Matched":1027,"XCorr":3.23,"X..Protein.Groups":1,"Percolator.q.Value":0.00035,"Percolator.PEP":2.688e-06,"Percolator.SVMScore":2.915,"Precursor.Area":687120.125,"Apex.RT..min.":16.04,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (J4)","Search.ID":"J","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].mSEGItDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); T6(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.114,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1096.46962,"Contaminant":"False","Aligned.m.z..Da.":1096.46958,"MH...Da.":2191.93197,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":1.88,"Deltam.z..Da.":0.00207,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":394496.03125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":15.518,"First.Scan":15513,"Last.Scan":15513,"Master.Scan.s.":15502,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_03.raw","File.ID":"F27","Quan.Info":"","Peptides.Matched":2068,"XCorr":5.35,"X..Protein.Groups":1,"Percolator.q.Value":0.00035,"Percolator.PEP":5.483e-08,"Percolator.SVMScore":4.026,"Precursor.Area":751018.1875,"Apex.RT..min.":15.54,"ptmRS..Binomial.Peptide.Score":454.3,"ptmRS..Isoform.Confidence.Probability":0.996,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(6): 512.24; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+-H(3) O(4) P(9): 869.39; b1+-H(3) O(4) P(10): 998.43; b1+(3): 259.09; b1+(4): 316.11; b1+(5): 429.20","ptmRS..Best.Site.Probabilities":"T6(Phospho): 99.61","ptmRS..Phospho.Site.Probabilities":"S(2): 0.4; T(6): 99.6; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (J4)","Search.ID":"J","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].mSEGItDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); T6(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.176,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":731.31544,"Contaminant":"False","Aligned.m.z..Da.":731.31541,"MH...Da.":2191.93178,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":1.79,"Deltam.z..Da.":0.00131,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":446749.65625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":19.27756,"Ion.Inject.Time..ms.":22,"RT..min.":15.5174,"First.Scan":15512,"Last.Scan":15512,"Master.Scan.s.":15502,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_03.raw","File.ID":"F27","Quan.Info":"","Peptides.Matched":2035,"XCorr":3.75,"X..Protein.Groups":1,"Percolator.q.Value":0.00035,"Percolator.PEP":8.71e-06,"Percolator.SVMScore":2.581,"Precursor.Area":579752.25,"Apex.RT..min.":15.53,"ptmRS..Binomial.Peptide.Score":390.42,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+-H(3) O(4) P(9): 869.39; b1+-H(3) O(4) P(10): 998.43; b1+(2): 130.05; b1+(3): 259.09; b1+(4): 316.11; b1+(5): 429.20; b2+(3): 130.05","ptmRS..Best.Site.Probabilities":"T6(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; T(6): 100.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (J4)","Search.ID":"J","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0478,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40932,"Contaminant":"False","Aligned.m.z..Da.":967.40922,"MH...Da.":2900.2134,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":4.85,"Deltam.z..Da.":0.00469,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":510400.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":15.8906,"First.Scan":15985,"Last.Scan":15985,"Master.Scan.s.":15983,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_03.raw","File.ID":"F27","Quan.Info":"","Peptides.Matched":2750,"XCorr":5.02,"X..Protein.Groups":1,"Percolator.q.Value":0.00035,"Percolator.PEP":1.356e-06,"Percolator.SVMScore":3.11,"ptmRS..Binomial.Peptide.Score":374.44,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y2+-H(3) O(4) P(14): 811.37; b1+(12): 1344.56","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (J4)","Search.ID":"J","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LQETNPEEVPKFEK","Annotated.Sequence":"[K].LQETNPEEVPKFEK.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.5838,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":563.29002,"Contaminant":"False","Aligned.m.z..Da.":563.29003,"MH...Da.":1687.85553,"Theo..MH...Da.":1687.85374,"DeltaM..ppm.":1.06,"Deltam.z..Da.":0.0006,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3918406.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":30.03778,"Ion.Inject.Time..ms.":22,"RT..min.":9.2993,"First.Scan":7449,"Last.Scan":7449,"Master.Scan.s.":7442,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_03.raw","File.ID":"F27","Quan.Info":"","Peptides.Matched":852,"XCorr":1.85,"X..Protein.Groups":1,"Percolator.q.Value":0.00035,"Percolator.PEP":0.00005,"Percolator.SVMScore":2.11,"Precursor.Area":2404492,"Apex.RT..min.":9.3,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (J4)","Search.ID":"J","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KDELTLEGIK","Annotated.Sequence":"[K].KDELTLEGIK.[Q]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5193,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":573.32385,"Contaminant":"False","Aligned.m.z..Da.":573.32386,"MH...Da.":1145.64042,"Theo..MH...Da.":1145.64123,"DeltaM..ppm.":-0.71,"Deltam.z..Da.":-0.00041,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2546524,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":11.80076,"Ion.Inject.Time..ms.":22,"RT..min.":10.2519,"First.Scan":8607,"Last.Scan":8607,"Master.Scan.s.":8599,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_03.raw","File.ID":"F27","Quan.Info":"","Peptides.Matched":339,"XCorr":3.89,"X..Protein.Groups":1,"Percolator.q.Value":0.00035,"Percolator.PEP":1.717e-06,"Percolator.SVMScore":3.043,"Precursor.Area":3722124.25,"Apex.RT..min.":10.26,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (J4)","Search.ID":"J","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"APQALMLAPTR","Annotated.Sequence":"[K].APQALmLAPTR.[E]","Modifications":"M6(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7338,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":592.82569,"Contaminant":"False","Aligned.m.z..Da.":592.82571,"MH...Da.":1184.64411,"Theo..MH...Da.":1184.6456,"DeltaM..ppm.":-1.26,"Deltam.z..Da.":-0.00075,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1799973.125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":21.33843,"Ion.Inject.Time..ms.":22,"RT..min.":10.172,"First.Scan":8506,"Last.Scan":8506,"Master.Scan.s.":8495,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_03.raw","File.ID":"F27","Quan.Info":"","Peptides.Matched":380,"XCorr":3.08,"X..Protein.Groups":1,"Percolator.q.Value":0.00035,"Percolator.PEP":9.853e-06,"Percolator.SVMScore":2.545,"Precursor.Area":3645501.75,"Apex.RT..min.":10.19,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (J4)","Search.ID":"J","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"AGNFALHPEVVR","Annotated.Sequence":"[R].AGNFALHPEVVR.[E]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q03558","Master.Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","Protein.Accessions":"Q03558","Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.6733,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":437.23801,"Contaminant":"False","Aligned.m.z..Da.":437.23802,"MH...Da.":1309.69948,"Theo..MH...Da.":1309.70114,"DeltaM..ppm.":-1.27,"Deltam.z..Da.":-0.00055,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2847503.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":7.28845,"Ion.Inject.Time..ms.":22,"RT..min.":10.0573,"First.Scan":8361,"Last.Scan":8361,"Master.Scan.s.":8352,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_03.raw","File.ID":"F27","Quan.Info":"","Peptides.Matched":464,"XCorr":2.51,"X..Protein.Groups":1,"Percolator.q.Value":0.00035,"Percolator.PEP":7.566e-06,"Percolator.SVMScore":2.621,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (J4)","Search.ID":"J","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"HSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[R].HSDsYsENETNHTNVPISSTGGTNNK.[T]","Modifications":"S4(Phospho); S6(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":0,"Charge":4,"Original.Precursor.Charge":4,"DeltaScore":0.0663,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":738.29253,"Contaminant":"False","Aligned.m.z..Da.":738.29256,"MH...Da.":2950.1483,"Theo..MH...Da.":2950.15241,"DeltaM..ppm.":-1.39,"Deltam.z..Da.":-0.00103,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2545589.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":71.2906,"Ion.Inject.Time..ms.":22,"RT..min.":8.0468,"First.Scan":6000,"Last.Scan":6000,"Master.Scan.s.":5999,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_03.raw","File.ID":"F27","Quan.Info":"","Peptides.Matched":973,"XCorr":3.62,"X..Protein.Groups":1,"Percolator.q.Value":0.00035,"Percolator.PEP":0.00003,"Percolator.SVMScore":2.27,"ptmRS..Binomial.Peptide.Score":282.09,"ptmRS..Isoform.Confidence.Probability":0.009,"ptmRS..Isoform.Group.Report":"b2+-H(3) O(4) P(14): 839.79; b2+-H(3) O(4) P(15): 889.33; b2+-H(3) O(4) P(17): 994.39; y2+- -2H(3) O(4) P -2H(3) O(4) P(20): 1039.48","ptmRS..Best.Site.Probabilities":"T10(Phospho): 94.32; T13(Phospho): 94.32","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(4): 3.8; Y(5): 3.8; S(6): 3.8; T(10): 94.3; T(13): 94.3; S(18): 0.0; S(19): 0.0; T(20): 0.0; T(23): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (J4)","Search.ID":"J","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"HSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[R].HSDsYsENETNHTNVPISSTGGTNNK.[T]","Modifications":"S4(Phospho); S6(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.132,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":984.05628,"Contaminant":"False","Aligned.m.z..Da.":984.05632,"MH...Da.":2950.1543,"Theo..MH...Da.":2950.15241,"DeltaM..ppm.":0.64,"Deltam.z..Da.":0.00063,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2260827,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":8.0324,"First.Scan":5984,"Last.Scan":5984,"Master.Scan.s.":5973,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_03.raw","File.ID":"F27","Quan.Info":"","Peptides.Matched":1144,"XCorr":6.82,"X..Protein.Groups":1,"Percolator.q.Value":0.00035,"Percolator.PEP":1.83e-08,"Percolator.SVMScore":4.339,"Precursor.Area":7059719.5,"Apex.RT..min.":8.04,"ptmRS..Binomial.Peptide.Score":693.94,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(6): 739.21; y2+-H(3) O(4) P(25): 1358.06; y2+-H(3) O(4) P(23): 1257.03; y2+-H(3) O(4) P(22): 1173.53; y2+-H(3) O(4) P(21): 1092.00; b1+- -2H(3) O(4) P -2H(3) O(4) P(6): 641.23; b1+- -2H(3) O(4) P -2H(3) O(4) P(11): 1228.45; b1+- -2H(3) O(4) P -2H(3) O(4) P(15): 1679.67; b2+- -2H(3) O(4) P -2H(3) O(4) P(13): 733.78; b2+- -2H(3) O(4) P -2H(3) O(4) P(14): 790.80; b2+- -2H(3) O(4) P -2H(3) O(4) P(15): 840.34","ptmRS..Best.Site.Probabilities":"S4(Phospho): 100; S6(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(4): 100.0; Y(5): 0.0; S(6): 100.0; T(10): 0.0; T(13): 0.0; S(18): 0.0; S(19): 0.0; T(20): 0.0; T(23): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (J4)","Search.ID":"J","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"APQALMLAPTR","Annotated.Sequence":"[K].APQALMLAPTR.[E]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6311,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":584.82916,"Contaminant":"False","Aligned.m.z..Da.":584.82916,"MH...Da.":1168.65104,"Theo..MH...Da.":1168.65069,"DeltaM..ppm.":0.3,"Deltam.z..Da.":0.00018,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":888949.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":63.62227,"Ion.Inject.Time..ms.":22,"RT..min.":11.6287,"First.Scan":10440,"Last.Scan":10440,"Master.Scan.s.":10432,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_03.raw","File.ID":"F27","Quan.Info":"","Peptides.Matched":162,"XCorr":3.09,"X..Protein.Groups":1,"Percolator.q.Value":0.00035,"Percolator.PEP":0.00002,"Percolator.SVMScore":2.372,"Precursor.Area":696956.5,"Apex.RT..min.":11.62,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (J4)","Search.ID":"J","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MGSINNNFNTNNNSNTDLDRDWK","Annotated.Sequence":"[-].mGSINNNFNTNNNsNTDLDRDWK.[T]","Modifications":"N-Term(Prot)(Met-loss); S14(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P39517","Master.Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","Protein.Accessions":"P39517","Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.067,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":878.36914,"Contaminant":"False","Aligned.m.z..Da.":878.36915,"MH...Da.":2633.09286,"Theo..MH...Da.":2633.10123,"DeltaM..ppm.":-3.18,"Deltam.z..Da.":-0.00279,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1606048.625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":18.98072,"Ion.Inject.Time..ms.":22,"RT..min.":11.2961,"First.Scan":9984,"Last.Scan":9984,"Master.Scan.s.":9977,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_03.raw","File.ID":"F27","Quan.Info":"","Peptides.Matched":2009,"XCorr":5.37,"X..Protein.Groups":1,"Percolator.q.Value":0.00035,"Percolator.PEP":7.735e-09,"Percolator.SVMScore":4.584,"Precursor.Area":4063140.75,"Apex.RT..min.":11.31,"ptmRS..Binomial.Peptide.Score":498.03,"ptmRS..Isoform.Confidence.Probability":0.994,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(13): 1573.70; y1+-H(3) O(4) P(12): 1459.66; y1+-H(3) O(4) P(11): 1345.61; y1+-H(3) O(4) P(10): 1231.57; y2+-H(3) O(4) P(19): 1139.50; y2+-H(3) O(4) P(18): 1082.48; y2+-H(3) O(4) P(16): 968.43; y2+-H(3) O(4) P(15): 894.90; y2+-H(3) O(4) P(14): 837.88; y2+-H(3) O(4) P(13): 787.35; y2+-H(3) O(4) P(12): 730.33; y2+-H(3) O(4) P(11): 673.31; y2+-H(3) O(4) P(10): 616.29; y1+(9): 1162.55","ptmRS..Best.Site.Probabilities":"S14(Phospho): 99.4","ptmRS..Phospho.Site.Probabilities":"S(3): 0.0; T(10): 0.0; S(14): 99.4; T(16): 0.6"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Q4)","Search.ID":"Q","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MGSINNNFNTNNNSNTDLDRDWK","Annotated.Sequence":"[-].mGSINNNFNTNNNsNTDLDRDWK.[T]","Modifications":"N-Term(Prot)(Met-loss); S14(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P39517","Master.Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","Protein.Accessions":"P39517","Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0191,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":878.37232,"Contaminant":"False","Aligned.m.z..Da.":878.37231,"MH...Da.":2633.10242,"Theo..MH...Da.":2633.10123,"DeltaM..ppm.":0.46,"Deltam.z..Da.":0.0004,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":862036.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":11.2803,"First.Scan":9932,"Last.Scan":9932,"Master.Scan.s.":9924,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_02.raw","File.ID":"F20","Quan.Info":"","Peptides.Matched":2279,"XCorr":5.23,"X..Protein.Groups":1,"Percolator.q.Value":0.00036,"Percolator.PEP":7.291e-11,"Percolator.SVMScore":4.407,"Precursor.Area":4169471.75,"Apex.RT..min.":11.3,"ptmRS..Binomial.Peptide.Score":528.52,"ptmRS..Isoform.Confidence.Probability":0.995,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(11): 1345.61; y1+-H(3) O(4) P(10): 1231.57; y2+-H(3) O(4) P(19): 1139.50; y2+-H(3) O(4) P(18): 1082.48; y2+-H(3) O(4) P(16): 968.43; y2+-H(3) O(4) P(15): 894.90; y2+-H(3) O(4) P(14): 837.88; y2+-H(3) O(4) P(13): 787.35; y2+-H(3) O(4) P(12): 730.33; y2+-H(3) O(4) P(11): 673.31; y2+-H(3) O(4) P(10): 616.29; y1+(9): 1162.55","ptmRS..Best.Site.Probabilities":"S14(Phospho): 99.46","ptmRS..Phospho.Site.Probabilities":"S(3): 0.0; T(10): 0.0; S(14): 99.5; T(16): 0.5"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Q4)","Search.ID":"Q","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DFLLKPELSR","Annotated.Sequence":"[K].DFLLKPELSR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.6342,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":406.56773,"Contaminant":"False","Aligned.m.z..Da.":406.56787,"MH...Da.":1217.68864,"Theo..MH...Da.":1217.68885,"DeltaM..ppm.":-0.17,"Deltam.z..Da.":-0.00007,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":782204.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":2.60665,"Ion.Inject.Time..ms.":22,"RT..min.":13.1519,"First.Scan":12462,"Last.Scan":12462,"Master.Scan.s.":12459,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_02.raw","File.ID":"F20","Quan.Info":"","Peptides.Matched":265,"XCorr":2.57,"X..Protein.Groups":1,"Percolator.q.Value":0.00036,"Percolator.PEP":9.28e-06,"Percolator.SVMScore":2.046,"Precursor.Area":477382.125,"Apex.RT..min.":13.15,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Q4)","Search.ID":"Q","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"APQALMLAPTR","Annotated.Sequence":"[K].APQALMLAPTR.[E]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":3,"DeltaScore":0.6864,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":584.82885,"Contaminant":"False","Aligned.m.z..Da.":584.82884,"MH...Da.":1168.65043,"Theo..MH...Da.":1168.65069,"DeltaM..ppm.":-0.23,"Deltam.z..Da.":-0.00013,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":661279.625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":84.45444,"Ion.Inject.Time..ms.":22,"RT..min.":11.6227,"First.Scan":10396,"Last.Scan":10396,"Master.Scan.s.":10392,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_02.raw","File.ID":"F20","Quan.Info":"","Peptides.Matched":164,"XCorr":1.69,"X..Protein.Groups":1,"Percolator.q.Value":0.00036,"Percolator.PEP":5.465e-06,"Percolator.SVMScore":2.152,"Precursor.Area":537662.375,"Apex.RT..min.":11.63,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Q4)","Search.ID":"Q","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"TAVFYGGTPISK","Annotated.Sequence":"[K].TAVFYGGTPISK.[D]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.4363,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":620.83114,"Contaminant":"False","Aligned.m.z..Da.":620.83114,"MH...Da.":1240.655,"Theo..MH...Da.":1240.65721,"DeltaM..ppm.":-1.79,"Deltam.z..Da.":-0.00111,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":732036.4375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":62.12711,"Ion.Inject.Time..ms.":22,"RT..min.":11.1313,"First.Scan":9731,"Last.Scan":9731,"Master.Scan.s.":9729,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_02.raw","File.ID":"F20","Quan.Info":"","Peptides.Matched":393,"XCorr":2.59,"X..Protein.Groups":1,"Percolator.q.Value":0.00036,"Percolator.PEP":0.00004,"Percolator.SVMScore":1.739,"Precursor.Area":552978.0625,"Apex.RT..min.":11.13,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Q4)","Search.ID":"Q","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"AIMPIIEGHDVLAQAQSGTGK","Annotated.Sequence":"[R].AImPIIEGHDVLAQAQSGTGK.[T]","Modifications":"M3(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.7107,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":718.03918,"Contaminant":"False","Aligned.m.z..Da.":718.03936,"MH...Da.":2152.103,"Theo..MH...Da.":2152.10668,"DeltaM..ppm.":-1.71,"Deltam.z..Da.":-0.00123,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":713841.8125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":17.15128,"Ion.Inject.Time..ms.":22,"RT..min.":12.7574,"First.Scan":11945,"Last.Scan":11945,"Master.Scan.s.":11939,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_02.raw","File.ID":"F20","Quan.Info":"","Peptides.Matched":1027,"XCorr":4.84,"X..Protein.Groups":1,"Percolator.q.Value":0.00036,"Percolator.PEP":3.442e-12,"Percolator.SVMScore":5.021,"Precursor.Area":1087808.125,"Apex.RT..min.":12.77,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Q4)","Search.ID":"Q","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.4116,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40367,"Contaminant":"False","Aligned.m.z..Da.":967.40343,"MH...Da.":2900.19647,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":-0.99,"Deltam.z..Da.":-0.00095,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":143403.64062,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":7.19554,"Ion.Inject.Time..ms.":22,"RT..min.":16.54,"First.Scan":16701,"Last.Scan":16701,"Master.Scan.s.":16693,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_02.raw","File.ID":"F20","Quan.Info":"","Peptides.Matched":2357,"XCorr":3.96,"X..Protein.Groups":1,"Percolator.q.Value":0.00036,"Percolator.PEP":2.095e-07,"Percolator.SVMScore":2.808,"ptmRS..Binomial.Peptide.Score":331.38,"ptmRS..Isoform.Confidence.Probability":0.5,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"Y12(Phospho): 50; S13(Phospho): 50","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 50.0; S(13): 50.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Q4)","Search.ID":"Q","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MFILDEADEMLSSGFK","Annotated.Sequence":"[K].mFILDEADEmLSSGFK.[E]","Modifications":"M1(Oxidation); M10(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7677,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":932.91805,"Contaminant":"False","Aligned.m.z..Da.":932.91778,"MH...Da.":1864.82884,"Theo..MH...Da.":1864.83433,"DeltaM..ppm.":-2.95,"Deltam.z..Da.":-0.00275,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":560063.4375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":13.54069,"Ion.Inject.Time..ms.":22,"RT..min.":16.6931,"First.Scan":16893,"Last.Scan":16893,"Master.Scan.s.":16890,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_02.raw","File.ID":"F20","Quan.Info":"","Peptides.Matched":1754,"XCorr":3.1,"X..Protein.Groups":1,"Percolator.q.Value":0.00036,"Percolator.PEP":4.194e-09,"Percolator.SVMScore":3.593,"Precursor.Area":423627.1875,"Apex.RT..min.":16.7,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Q4)","Search.ID":"Q","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].msEGITDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S2(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":3,"DeltaScore":0.1554,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1096.46612,"Contaminant":"False","Aligned.m.z..Da.":1096.4662,"MH...Da.":2191.92497,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":-1.31,"Deltam.z..Da.":-0.00144,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":13606298,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":9.20141,"Ion.Inject.Time..ms.":22,"RT..min.":15.776,"First.Scan":15749,"Last.Scan":15749,"Master.Scan.s.":15744,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_02.raw","File.ID":"F20","Quan.Info":"","Peptides.Matched":1913,"XCorr":3.99,"X..Protein.Groups":1,"Percolator.q.Value":0.00036,"Percolator.PEP":9.406e-09,"Percolator.SVMScore":3.431,"Precursor.Area":14209928,"Apex.RT..min.":15.78,"ptmRS..Binomial.Peptide.Score":347.66,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(2): 112.04; b1+-H(3) O(4) P(3): 241.08; b1+-H(3) O(4) P(4): 298.10; b1+-H(3) O(4) P(5): 411.19; b1+-H(3) O(4) P(6): 512.24; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+-H(3) O(4) P(9): 869.39","ptmRS..Best.Site.Probabilities":"S2(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 100.0; T(6): 0.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Q4)","Search.ID":"Q","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ILISTDLLAR","Annotated.Sequence":"[R].ILISTDLLAR.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5143,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":557.84551,"Contaminant":"False","Aligned.m.z..Da.":557.84558,"MH...Da.":1114.68374,"Theo..MH...Da.":1114.68304,"DeltaM..ppm.":0.63,"Deltam.z..Da.":0.00035,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1140291,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":19.60695,"Ion.Inject.Time..ms.":22,"RT..min.":15.5896,"First.Scan":15518,"Last.Scan":15518,"Master.Scan.s.":15508,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_02.raw","File.ID":"F20","Quan.Info":"","Peptides.Matched":124,"XCorr":2.8,"X..Protein.Groups":1,"Percolator.q.Value":0.00036,"Percolator.PEP":0.00007,"Percolator.SVMScore":1.651,"Precursor.Area":1887916.625,"Apex.RT..min.":15.6,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Q4)","Search.ID":"Q","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].mSEGItDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); T6(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.1778,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":731.31421,"Contaminant":"False","Aligned.m.z..Da.":731.31433,"MH...Da.":2191.92809,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":0.11,"Deltam.z..Da.":0.00008,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":312176.125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":15.5268,"First.Scan":15439,"Last.Scan":15439,"Master.Scan.s.":15433,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_02.raw","File.ID":"F20","Quan.Info":"","Peptides.Matched":1980,"XCorr":3.88,"X..Protein.Groups":1,"Percolator.q.Value":0.00036,"Percolator.PEP":9.111e-09,"Percolator.SVMScore":3.437,"Precursor.Area":260364.1875,"Apex.RT..min.":15.53,"ptmRS..Binomial.Peptide.Score":389.18,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+-H(3) O(4) P(9): 869.39; b1+(2): 130.05; b1+(3): 259.09; b1+(4): 316.11; b1+(5): 429.20; b2+(3): 130.05","ptmRS..Best.Site.Probabilities":"T6(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; T(6): 100.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Q4)","Search.ID":"Q","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].mSEGItDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); T6(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1101,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1096.4676,"Contaminant":"False","Aligned.m.z..Da.":1096.46777,"MH...Da.":2191.92792,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":0.04,"Deltam.z..Da.":0.00004,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":310158.5625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":27.71749,"Ion.Inject.Time..ms.":22,"RT..min.":15.5197,"First.Scan":15431,"Last.Scan":15431,"Master.Scan.s.":15420,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_02.raw","File.ID":"F20","Quan.Info":"","Peptides.Matched":1964,"XCorr":4.54,"X..Protein.Groups":1,"Percolator.q.Value":0.00036,"Percolator.PEP":2.265e-09,"Percolator.SVMScore":3.717,"Precursor.Area":354136.0625,"Apex.RT..min.":15.53,"ptmRS..Binomial.Peptide.Score":461.33,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(6): 512.24; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+-H(3) O(4) P(9): 869.39; b1+-H(3) O(4) P(10): 998.43; y2+-H(3) O(4) P(14): 833.38; b1+(3): 259.09; b1+(4): 316.11; b1+(5): 429.20","ptmRS..Best.Site.Probabilities":"T6(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; T(6): 100.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Q4)","Search.ID":"Q","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.3932,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40643,"Contaminant":"False","Aligned.m.z..Da.":967.40635,"MH...Da.":2900.20474,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":1.87,"Deltam.z..Da.":0.0018,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":213670.79688,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":29.69371,"Ion.Inject.Time..ms.":22,"RT..min.":16.1505,"First.Scan":16218,"Last.Scan":16218,"Master.Scan.s.":16217,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_02.raw","File.ID":"F20","Quan.Info":"","Peptides.Matched":2545,"XCorr":3.84,"X..Protein.Groups":1,"Percolator.q.Value":0.00036,"Percolator.PEP":1.726e-06,"Percolator.SVMScore":2.384,"ptmRS..Binomial.Peptide.Score":321.73,"ptmRS..Isoform.Confidence.Probability":0.992,"ptmRS..Isoform.Group.Report":"y2+-H(3) O(4) P(14): 811.37","ptmRS..Best.Site.Probabilities":"S13(Phospho): 99.21","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.8; S(13): 99.2; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Q4)","Search.ID":"Q","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].msEGITDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S2(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1133,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1096.46598,"Contaminant":"False","Aligned.m.z..Da.":1096.46595,"MH...Da.":2191.92469,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":-1.44,"Deltam.z..Da.":-0.00157,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":382438.9375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":16.0339,"First.Scan":16073,"Last.Scan":16073,"Master.Scan.s.":16072,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_02.raw","File.ID":"F20","Quan.Info":"","Peptides.Matched":1843,"XCorr":3.62,"X..Protein.Groups":1,"Percolator.q.Value":0.00036,"Percolator.PEP":8.991e-08,"Percolator.SVMScore":2.977,"Precursor.Area":14209928,"Apex.RT..min.":15.78,"ptmRS..Binomial.Peptide.Score":436.91,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(2): 112.04; b1+-H(3) O(4) P(3): 241.08; b1+-H(3) O(4) P(4): 298.10; b1+-H(3) O(4) P(5): 411.19; b1+-H(3) O(4) P(6): 512.24; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+-H(3) O(4) P(9): 869.39; b1+-H(3) O(4) P(10): 998.43","ptmRS..Best.Site.Probabilities":"S2(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 100.0; T(6): 0.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Q4)","Search.ID":"Q","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FDDMELDENLLR","Annotated.Sequence":"[K].FDDMELDENLLR.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.586,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":755.34771,"Contaminant":"False","Aligned.m.z..Da.":755.34768,"MH...Da.":1509.68814,"Theo..MH...Da.":1509.68898,"DeltaM..ppm.":-0.56,"Deltam.z..Da.":-0.00042,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":649687.3125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":28.64731,"Ion.Inject.Time..ms.":22,"RT..min.":16.0274,"First.Scan":16066,"Last.Scan":16066,"Master.Scan.s.":16059,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_02.raw","File.ID":"F20","Quan.Info":"","Peptides.Matched":1050,"XCorr":3.43,"X..Protein.Groups":1,"Percolator.q.Value":0.00036,"Percolator.PEP":5.711e-08,"Percolator.SVMScore":3.069,"Precursor.Area":499595.5,"Apex.RT..min.":16.03,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Q4)","Search.ID":"Q","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.3349,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40801,"Contaminant":"False","Aligned.m.z..Da.":967.40767,"MH...Da.":2900.20948,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":3.5,"Deltam.z..Da.":0.00338,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":210367.40625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":7.14775,"Ion.Inject.Time..ms.":22,"RT..min.":17.0462,"First.Scan":17338,"Last.Scan":17338,"Master.Scan.s.":17337,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_02.raw","File.ID":"F20","Quan.Info":"","Peptides.Matched":2644,"XCorr":4.18,"X..Protein.Groups":1,"Percolator.q.Value":0.00036,"Percolator.PEP":9.773e-07,"Percolator.SVMScore":2.498,"ptmRS..Binomial.Peptide.Score":315.18,"ptmRS..Isoform.Confidence.Probability":0.5,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"Y12(Phospho): 50; S13(Phospho): 50","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 50.0; S(13): 50.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Q4)","Search.ID":"Q","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DIFSNDELLSDAYDAK","Annotated.Sequence":"[K].DIFSNDELLSDAYDAK.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7606,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":908.41818,"Contaminant":"False","Aligned.m.z..Da.":908.41786,"MH...Da.":1815.82909,"Theo..MH...Da.":1815.82831,"DeltaM..ppm.":0.43,"Deltam.z..Da.":0.00039,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":428429.34375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":19.37436,"Ion.Inject.Time..ms.":22,"RT..min.":17.0704,"First.Scan":17367,"Last.Scan":17367,"Master.Scan.s.":17361,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_02.raw","File.ID":"F20","Quan.Info":"","Peptides.Matched":1570,"XCorr":4.47,"X..Protein.Groups":1,"Percolator.q.Value":0.00036,"Percolator.PEP":2.891e-10,"Percolator.SVMScore":4.131,"Precursor.Area":2146169.75,"Apex.RT..min.":17.09,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Q4)","Search.ID":"Q","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0192,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40426,"Contaminant":"False","Aligned.m.z..Da.":967.40441,"MH...Da.":2900.19823,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":-0.38,"Deltam.z..Da.":-0.00037,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":712225.5625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":6.78852,"Ion.Inject.Time..ms.":22,"RT..min.":15.5033,"First.Scan":15408,"Last.Scan":15408,"Master.Scan.s.":15407,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_02.raw","File.ID":"F20","Quan.Info":"","Peptides.Matched":2452,"XCorr":4.68,"X..Protein.Groups":1,"Percolator.q.Value":0.00036,"Percolator.PEP":8.39e-09,"Percolator.SVMScore":3.454,"Precursor.Area":32169858,"Apex.RT..min.":15.09,"ptmRS..Binomial.Peptide.Score":370.78,"ptmRS..Isoform.Confidence.Probability":0.992,"ptmRS..Isoform.Group.Report":"b1+(12): 1344.56","ptmRS..Best.Site.Probabilities":"S13(Phospho): 99.22","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.8; S(13): 99.2; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Q4)","Search.ID":"Q","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MGSINNNFNTNNNSNTDLDRDWK","Annotated.Sequence":"[-].mGSINNNFNTNNNsNTDLDRDWK.[T]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S14(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P39517","Master.Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","Protein.Accessions":"P39517","Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.0631,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1338.0613,"Contaminant":"False","Aligned.m.z..Da.":1338.06178,"MH...Da.":2675.11533,"Theo..MH...Da.":2675.11179,"DeltaM..ppm.":1.32,"Deltam.z..Da.":0.00177,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":465906.71875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":13.5628,"First.Scan":12989,"Last.Scan":12989,"Master.Scan.s.":12979,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_02.raw","File.ID":"F20","Quan.Info":"","Peptides.Matched":2342,"XCorr":4.44,"X..Protein.Groups":1,"Percolator.q.Value":0.00036,"Percolator.PEP":2.045e-08,"Percolator.SVMScore":3.275,"ptmRS..Binomial.Peptide.Score":560.31,"ptmRS..Isoform.Confidence.Probability":0.996,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(16): 1935.86; y1+-H(3) O(4) P(14): 1674.75; y1+-H(3) O(4) P(13): 1573.70; y1+-H(3) O(4) P(12): 1459.66; y1+-H(3) O(4) P(10): 1231.57; b2+-H(3) O(4) P(19): 987.41; y2+-H(3) O(4) P(19): 1139.50; y2+-H(3) O(4) P(17): 1025.45; y1+(9): 1162.55; y1+(8): 1048.51","ptmRS..Best.Site.Probabilities":"S14(Phospho): 99.64","ptmRS..Phospho.Site.Probabilities":"S(3): 0.0; T(10): 0.0; S(14): 99.6; T(16): 0.4"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Q4)","Search.ID":"Q","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"TGTFSIAALQR","Annotated.Sequence":"[K].TGTFSIAALQR.[I]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.737,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":582.82205,"Contaminant":"False","Aligned.m.z..Da.":582.82227,"MH...Da.":1164.63683,"Theo..MH...Da.":1164.63715,"DeltaM..ppm.":-0.27,"Deltam.z..Da.":-0.00016,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1018051.875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":19.97178,"Ion.Inject.Time..ms.":22,"RT..min.":13.3569,"First.Scan":12725,"Last.Scan":12725,"Master.Scan.s.":12719,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_02.raw","File.ID":"F20","Quan.Info":"","Peptides.Matched":364,"XCorr":2.89,"X..Protein.Groups":1,"Percolator.q.Value":0.00036,"Percolator.PEP":0.00001,"Percolator.SVMScore":1.997,"Precursor.Area":1821428.5,"Apex.RT..min.":13.37,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Q4)","Search.ID":"Q","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.079,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":953.40265,"Contaminant":"False","Aligned.m.z..Da.":953.40294,"MH...Da.":2858.1934,"Theo..MH...Da.":2858.18876,"DeltaM..ppm.":1.62,"Deltam.z..Da.":0.00155,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":631836.125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":33.94739,"Ion.Inject.Time..ms.":22,"RT..min.":13.8438,"First.Scan":13344,"Last.Scan":13344,"Master.Scan.s.":13343,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_02.raw","File.ID":"F20","Quan.Info":"","Peptides.Matched":2515,"XCorr":5.57,"X..Protein.Groups":1,"Percolator.q.Value":0.00036,"Percolator.PEP":7.721e-09,"Percolator.SVMScore":3.471,"ptmRS..Binomial.Peptide.Score":355.5,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b2+-H(3) O(4) P(18): 993.90; b2+-H(3) O(4) P(21): 1171.01; y2+-H(3) O(4) P(14): 811.37; b1+(12): 1302.55","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Q4)","Search.ID":"Q","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":5,"DeltaScore":0.0943,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40468,"Contaminant":"False","Aligned.m.z..Da.":967.4049,"MH...Da.":2900.19948,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":0.05,"Deltam.z..Da.":0.00005,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":8493611,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":12.34766,"Ion.Inject.Time..ms.":19.119,"RT..min.":15.1346,"First.Scan":14951,"Last.Scan":14951,"Master.Scan.s.":14948,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_02.raw","File.ID":"F20","Quan.Info":"","Peptides.Matched":2478,"XCorr":7.21,"X..Protein.Groups":1,"Percolator.q.Value":0.00036,"Percolator.PEP":1.089e-15,"Percolator.SVMScore":6.804,"Precursor.Area":32169858,"Apex.RT..min.":15.09,"ptmRS..Binomial.Peptide.Score":813.2,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(13): 1413.58; b1+-H(3) O(4) P(14): 1528.61; b1+-H(3) O(4) P(15): 1642.65; b1+-H(3) O(4) P(17): 1899.75; b1+-H(3) O(4) P(18): 2028.79; y1+-H(3) O(4) P(15): 1750.78; y1+-H(3) O(4) P(14): 1621.73; y1+-H(3) O(4) P(13): 1458.67; b2+-H(3) O(4) P(16): 886.35; b2+-H(3) O(4) P(17): 950.38; b2+-H(3) O(4) P(18): 1014.90; y2+-H(3) O(4) P(14): 811.37; y2+-H(3) O(4) P(13): 729.84; b1+(12): 1344.56; y1+(13): 1556.65","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Q4)","Search.ID":"Q","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"GVFGYGFEEPSAIQQR","Annotated.Sequence":"[R].GVFGYGFEEPSAIQQR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6204,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":892.9322,"Contaminant":"False","Aligned.m.z..Da.":892.93241,"MH...Da.":1784.85712,"Theo..MH...Da.":1784.86022,"DeltaM..ppm.":-1.74,"Deltam.z..Da.":-0.00155,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":824485.5625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":39.49807,"Ion.Inject.Time..ms.":22,"RT..min.":15.0514,"First.Scan":14846,"Last.Scan":14846,"Master.Scan.s.":14844,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_02.raw","File.ID":"F20","Quan.Info":"","Peptides.Matched":1388,"XCorr":2.74,"X..Protein.Groups":1,"Percolator.q.Value":0.00036,"Percolator.PEP":1.125e-06,"Percolator.SVMScore":2.47,"Precursor.Area":981976.625,"Apex.RT..min.":15.03,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Q4)","Search.ID":"Q","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"GVFGYGFEEPSAIQQR","Annotated.Sequence":"[R].GVFGYGFEEPSAIQQR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6887,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":892.93281,"Contaminant":"False","Aligned.m.z..Da.":892.93302,"MH...Da.":1784.85834,"Theo..MH...Da.":1784.86022,"DeltaM..ppm.":-1.06,"Deltam.z..Da.":-0.00094,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":994381.4375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":18.16594,"Ion.Inject.Time..ms.":22,"RT..min.":15.0214,"First.Scan":14808,"Last.Scan":14808,"Master.Scan.s.":14805,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_02.raw","File.ID":"F20","Quan.Info":"","Peptides.Matched":1341,"XCorr":4.53,"X..Protein.Groups":1,"Percolator.q.Value":0.00036,"Percolator.PEP":9.889e-11,"Percolator.SVMScore":4.346,"Precursor.Area":981976.625,"Apex.RT..min.":15.03,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Q4)","Search.ID":"Q","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].mSEGITDIEEsQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S11(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1583,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1096.46612,"Contaminant":"False","Aligned.m.z..Da.":1096.46638,"MH...Da.":2191.92497,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":-1.31,"Deltam.z..Da.":-0.00144,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":363687.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":19.06848,"Ion.Inject.Time..ms.":22,"RT..min.":14.9942,"First.Scan":14775,"Last.Scan":14775,"Master.Scan.s.":14766,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_02.raw","File.ID":"F20","Quan.Info":"","Peptides.Matched":1913,"XCorr":2.78,"X..Protein.Groups":1,"Percolator.q.Value":0.00036,"Percolator.PEP":0.00003,"Percolator.SVMScore":1.825,"Precursor.Area":203220.71875,"Apex.RT..min.":15,"ptmRS..Binomial.Peptide.Score":295.79,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(14): 1665.76; y1+-H(3) O(4) P(13): 1564.71; y1+-H(3) O(4) P(10): 1207.56; y1+-H(3) O(4) P(9): 1078.52; y1+(7): 881.44; y1+(6): 768.35; y1+(5): 640.29","ptmRS..Best.Site.Probabilities":"S11(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; T(6): 0.0; S(11): 100.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Q4)","Search.ID":"Q","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0569,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40376,"Contaminant":"False","Aligned.m.z..Da.":967.40399,"MH...Da.":2900.19673,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":-0.9,"Deltam.z..Da.":-0.00087,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":237342.07812,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":14.9923,"First.Scan":14772,"Last.Scan":14772,"Master.Scan.s.":14766,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_02.raw","File.ID":"F20","Quan.Info":"","Peptides.Matched":2347,"XCorr":4.39,"X..Protein.Groups":1,"Percolator.q.Value":0.00036,"Percolator.PEP":6.5e-08,"Percolator.SVMScore":3.043,"Precursor.Area":32169858,"Apex.RT..min.":15.09,"ptmRS..Binomial.Peptide.Score":367.81,"ptmRS..Isoform.Confidence.Probability":0.994,"ptmRS..Isoform.Group.Report":"b1+(12): 1344.56","ptmRS..Best.Site.Probabilities":"S13(Phospho): 99.44","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.6; S(13): 99.4; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Q4)","Search.ID":"Q","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FTVSAIYSDLPQQER","Annotated.Sequence":"[K].FTVSAIYSDLPQQER.[D]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6925,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":877.4435,"Contaminant":"False","Aligned.m.z..Da.":877.44371,"MH...Da.":1753.87973,"Theo..MH...Da.":1753.87554,"DeltaM..ppm.":2.39,"Deltam.z..Da.":0.00209,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":379286.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":28.88765,"Ion.Inject.Time..ms.":22,"RT..min.":15.0161,"First.Scan":14803,"Last.Scan":14803,"Master.Scan.s.":14792,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_02.raw","File.ID":"F20","Quan.Info":"","Peptides.Matched":1088,"XCorr":3.74,"X..Protein.Groups":1,"Percolator.q.Value":0.00036,"Percolator.PEP":1.909e-09,"Percolator.SVMScore":3.751,"Precursor.Area":747950.125,"Apex.RT..min.":15.03,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Q4)","Search.ID":"Q","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FFISNPDLVDRLEK","Annotated.Sequence":"[R].FFISNPDLVDRLEK.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q03558","Master.Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","Protein.Accessions":"Q03558","Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.6647,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":564.96968,"Contaminant":"False","Aligned.m.z..Da.":564.96979,"MH...Da.":1692.8945,"Theo..MH...Da.":1692.89555,"DeltaM..ppm.":-0.62,"Deltam.z..Da.":-0.00035,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":436436.53125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":49.77108,"Ion.Inject.Time..ms.":22,"RT..min.":15.3434,"First.Scan":15210,"Last.Scan":15210,"Master.Scan.s.":15199,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_02.raw","File.ID":"F20","Quan.Info":"","Peptides.Matched":556,"XCorr":1.7,"X..Protein.Groups":1,"Percolator.q.Value":0.00036,"Percolator.PEP":0.00004,"Percolator.SVMScore":1.773,"Precursor.Area":252817.125,"Apex.RT..min.":15.33,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Q4)","Search.ID":"Q","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0212,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40583,"Contaminant":"False","Aligned.m.z..Da.":967.40605,"MH...Da.":2900.20293,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":1.24,"Deltam.z..Da.":0.0012,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":206054.34375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":51.97127,"Ion.Inject.Time..ms.":22,"RT..min.":14.8603,"First.Scan":14619,"Last.Scan":14619,"Master.Scan.s.":14614,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_02.raw","File.ID":"F20","Quan.Info":"","Peptides.Matched":2567,"XCorr":4.24,"X..Protein.Groups":1,"Percolator.q.Value":0.00036,"Percolator.PEP":9.883e-09,"Percolator.SVMScore":3.421,"Precursor.Area":472653.40625,"Apex.RT..min.":14.83,"ptmRS..Binomial.Peptide.Score":432.52,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(14): 1621.73; b2+-H(3) O(4) P(18): 1014.90; y2+-H(3) O(4) P(14): 811.37; y2+-H(3) O(4) P(13): 729.84","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Q4)","Search.ID":"Q","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].mSEGITDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.687,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1056.48363,"Contaminant":"False","Aligned.m.z..Da.":1056.48386,"MH...Da.":2111.96,"Theo..MH...Da.":2111.96151,"DeltaM..ppm.":-0.72,"Deltam.z..Da.":-0.00076,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1057454.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":60.52768,"Ion.Inject.Time..ms.":22,"RT..min.":14.6726,"First.Scan":14386,"Last.Scan":14386,"Master.Scan.s.":14382,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_02.raw","File.ID":"F20","Quan.Info":"","Peptides.Matched":2325,"XCorr":3.61,"X..Protein.Groups":1,"Percolator.q.Value":0.00036,"Percolator.PEP":1.635e-09,"Percolator.SVMScore":3.783,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Q4)","Search.ID":"Q","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].mSEGITDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":3,"DeltaScore":0.7529,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1056.48168,"Contaminant":"False","Aligned.m.z..Da.":1056.48191,"MH...Da.":2111.95608,"Theo..MH...Da.":2111.96151,"DeltaM..ppm.":-2.57,"Deltam.z..Da.":-0.00271,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":338626.125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":84.11342,"Ion.Inject.Time..ms.":22,"RT..min.":14.6404,"First.Scan":14344,"Last.Scan":14344,"Master.Scan.s.":14343,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_02.raw","File.ID":"F20","Quan.Info":"","Peptides.Matched":2243,"XCorr":4.25,"X..Protein.Groups":1,"Percolator.q.Value":0.00036,"Percolator.PEP":1.019e-11,"Percolator.SVMScore":4.803,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Q4)","Search.ID":"Q","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LQETNPEEVPKFEK","Annotated.Sequence":"[K].LQETNPEEVPKFEK.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.6229,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":563.29059,"Contaminant":"False","Aligned.m.z..Da.":563.29063,"MH...Da.":1687.85723,"Theo..MH...Da.":1687.85374,"DeltaM..ppm.":2.07,"Deltam.z..Da.":0.00116,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2434990,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":48.02594,"Ion.Inject.Time..ms.":22,"RT..min.":9.304,"First.Scan":7463,"Last.Scan":7463,"Master.Scan.s.":7454,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_02.raw","File.ID":"F20","Quan.Info":"","Peptides.Matched":851,"XCorr":1.75,"X..Protein.Groups":1,"Percolator.q.Value":0.00036,"Percolator.PEP":0.0001,"Percolator.SVMScore":1.574,"Precursor.Area":1334552.25,"Apex.RT..min.":9.3,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Q4)","Search.ID":"Q","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"HSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[R].HSDsYsENETNHTNVPISSTGGTNNK.[T]","Modifications":"S4(Phospho); S6(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.1492,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":984.0561,"Contaminant":"False","Aligned.m.z..Da.":984.05626,"MH...Da.":2950.15375,"Theo..MH...Da.":2950.15241,"DeltaM..ppm.":0.45,"Deltam.z..Da.":0.00045,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":4066738,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":8.0321,"First.Scan":5976,"Last.Scan":5976,"Master.Scan.s.":5972,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_02.raw","File.ID":"F20","Quan.Info":"","Peptides.Matched":1174,"XCorr":6.57,"X..Protein.Groups":1,"Percolator.q.Value":0.00036,"Percolator.PEP":5.154e-11,"Percolator.SVMScore":4.477,"Precursor.Area":4127615.75,"Apex.RT..min.":8.04,"ptmRS..Binomial.Peptide.Score":633.25,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(4): 409.15; b1+-H(3) O(4) P(5): 572.21; b1+-H(3) O(4) P(6): 739.21; b1+-H(3) O(4) P(9): 1111.34; b1+-H(3) O(4) P(11): 1326.43; b2+-H(3) O(4) P(12): 732.25; b2+-H(3) O(4) P(14): 839.79; b2+-H(3) O(4) P(15): 889.33; y2+- -2H(3) O(4) P -2H(3) O(4) P(24): 1265.56; b1+(2): 225.10; b1+(3): 340.13","ptmRS..Best.Site.Probabilities":"S4(Phospho): 99.99; S6(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(4): 100.0; Y(5): 0.0; S(6): 100.0; T(10): 0.0; T(13): 0.0; S(18): 0.0; S(19): 0.0; T(20): 0.0; T(23): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Q4)","Search.ID":"Q","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"IQDPQMTGYVSTR","Annotated.Sequence":"[R].IQDPQmtGYVsTR.[Y]","Modifications":"M6(Oxidation); T7(Phospho); S11(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P32485","Master.Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","Protein.Accessions":"P32485","Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.0505,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":836.32694,"Contaminant":"False","Aligned.m.z..Da.":836.32706,"MH...Da.":1671.64661,"Theo..MH...Da.":1671.64853,"DeltaM..ppm.":-1.15,"Deltam.z..Da.":-0.00096,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2020962.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":10.29678,"Ion.Inject.Time..ms.":22,"RT..min.":7.7361,"First.Scan":5580,"Last.Scan":5580,"Master.Scan.s.":5569,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_02.raw","File.ID":"F20","Quan.Info":"","Peptides.Matched":742,"XCorr":2.97,"X..Protein.Groups":1,"Percolator.q.Value":0.00036,"Percolator.PEP":0.00002,"Percolator.SVMScore":1.869,"Precursor.Area":1959545.25,"Apex.RT..min.":7.74,"ptmRS..Binomial.Peptide.Score":336.65,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(11): 1332.53; y1+-H(3) O(4) P(10): 1217.50; y1+-H(3) O(4) P(8): 992.39; y1+-H(3) O(4) P(7): 845.36; y2+-H(3) O(4) P(11): 666.77; y2+-H(3) O(4) P(10): 609.25; y1+(4): 462.27; y1+(3): 363.20","ptmRS..Best.Site.Probabilities":"T7(Phospho): 100; Y9(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"T(7): 100.0; Y(9): 100.0; S(11): 0.0; T(12): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Q4)","Search.ID":"Q","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LQETNPEEVPK","Annotated.Sequence":"[K].LQETNPEEVPK.[F]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5017,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":642.32791,"Contaminant":"False","Aligned.m.z..Da.":642.32798,"MH...Da.":1283.64854,"Theo..MH...Da.":1283.64777,"DeltaM..ppm.":0.6,"Deltam.z..Da.":0.00039,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3999104,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":6.32302,"Ion.Inject.Time..ms.":22,"RT..min.":7.6334,"First.Scan":5430,"Last.Scan":5430,"Master.Scan.s.":5426,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_02.raw","File.ID":"F20","Quan.Info":"","Peptides.Matched":579,"XCorr":2.89,"X..Protein.Groups":1,"Percolator.q.Value":0.00036,"Percolator.PEP":0.00001,"Percolator.SVMScore":2.023,"Precursor.Area":6521421,"Apex.RT..min.":7.65,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Q4)","Search.ID":"Q","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"HSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[R].HSDSySENETNHTNVPISSTGGTNNK.[T]","Modifications":"Y5(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":0,"Charge":4,"Original.Precursor.Charge":4,"DeltaScore":0.0016,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":718.30244,"Contaminant":"False","Aligned.m.z..Da.":718.30252,"MH...Da.":2870.18793,"Theo..MH...Da.":2870.18608,"DeltaM..ppm.":0.65,"Deltam.z..Da.":0.00046,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1718155,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":1.49035,"Ion.Inject.Time..ms.":22,"RT..min.":7.6428,"First.Scan":5444,"Last.Scan":5444,"Master.Scan.s.":5439,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_02.raw","File.ID":"F20","Quan.Info":"","Peptides.Matched":2519,"XCorr":6.34,"X..Protein.Groups":1,"Percolator.q.Value":0.00036,"Percolator.PEP":3.917e-10,"Percolator.SVMScore":4.07,"Precursor.Area":5618344.5,"Apex.RT..min.":7.65,"ptmRS..Binomial.Peptide.Score":285.44,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(9): 1031.37; b1+-H(3) O(4) P(11): 1246.46; b1+-H(3) O(4) P(12): 1383.52; b2+-H(3) O(4) P(7): 394.65; b2+-H(3) O(4) P(8): 451.67; b2+-H(3) O(4) P(13): 742.79; b2+-H(3) O(4) P(14): 799.81; b2+-H(3) O(4) P(15): 849.34; b2+-H(3) O(4) P(17): 954.41; b1+(4): 427.16","ptmRS..Best.Site.Probabilities":"S6(Phospho): 99.36","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(4): 0.6; Y(5): 0.0; S(6): 99.4; T(10): 0.0; T(13): 0.0; S(18): 0.0; S(19): 0.0; T(20): 0.0; T(23): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Q4)","Search.ID":"Q","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"HSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[R].HSDSYsENETNHTNVPISSTGGTNNK.[T]","Modifications":"S6(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.003,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":957.39971,"Contaminant":"False","Aligned.m.z..Da.":957.39983,"MH...Da.":2870.18459,"Theo..MH...Da.":2870.18608,"DeltaM..ppm.":-0.52,"Deltam.z..Da.":-0.0005,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3783103,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":7.6417,"First.Scan":5442,"Last.Scan":5442,"Master.Scan.s.":5439,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_02.raw","File.ID":"F20","Quan.Info":"","Peptides.Matched":2477,"XCorr":6.56,"X..Protein.Groups":1,"Percolator.q.Value":0.00036,"Percolator.PEP":1.022e-11,"Percolator.SVMScore":4.802,"Precursor.Area":9813656,"Apex.RT..min.":7.65,"ptmRS..Binomial.Peptide.Score":464.61,"ptmRS..Isoform.Confidence.Probability":0.007,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(4): 409.15; b1+-H(3) O(4) P(5): 572.21; b1+-H(3) O(4) P(6): 659.24; b1+-H(3) O(4) P(11): 1246.46; b1+-H(3) O(4) P(12): 1383.52; b1+-H(3) O(4) P(15): 1697.68; b2+-H(3) O(4) P(13): 742.79; b2+-H(3) O(4) P(14): 799.81; b2+-H(3) O(4) P(15): 849.34","ptmRS..Best.Site.Probabilities":"S4(Phospho): 99.32","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(4): 99.3; Y(5): 0.0; S(6): 0.7; T(10): 0.0; T(13): 0.0; S(18): 0.0; S(19): 0.0; T(20): 0.0; T(23): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Q4)","Search.ID":"Q","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSSPLHRLSPTGK","Annotated.Sequence":"[R].mSsPLHRLsPTGK.[S]","Modifications":"M1(Oxidation); S3(Phospho); S9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.4913,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":529.56446,"Contaminant":"False","Aligned.m.z..Da.":529.56453,"MH...Da.":1586.67882,"Theo..MH...Da.":1586.67977,"DeltaM..ppm.":-0.6,"Deltam.z..Da.":-0.00032,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3182360,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":20.13834,"Ion.Inject.Time..ms.":22,"RT..min.":7.7057,"First.Scan":5535,"Last.Scan":5535,"Master.Scan.s.":5530,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_02.raw","File.ID":"F20","Quan.Info":"","Peptides.Matched":979,"XCorr":3.46,"X..Protein.Groups":1,"Percolator.q.Value":0.00036,"Percolator.PEP":0.00006,"Percolator.SVMScore":1.66,"Precursor.Area":5224876,"Apex.RT..min.":7.73,"ptmRS..Binomial.Peptide.Score":227.92,"ptmRS..Isoform.Confidence.Probability":0.993,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(8): 877.50; y2+-H(3) O(4) P(12): 671.34; y2+-H(3) O(4) P(10): 544.32; y2+-H(3) O(4) P(8): 439.25; y1+(4): 402.23","ptmRS..Best.Site.Probabilities":"S3(Phospho): 99.99; S9(Phospho): 99.28","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(3): 100.0; S(9): 99.3; T(11): 0.7"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Q4)","Search.ID":"Q","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"AAEAGETGAATSATEGDNNNNTAAGDKK","Annotated.Sequence":"[K].AAEAGETGAATSATEGDNNNNTAAGDKK.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.7371,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":879.39326,"Contaminant":"False","Aligned.m.z..Da.":879.39329,"MH...Da.":2636.16524,"Theo..MH...Da.":2636.16665,"DeltaM..ppm.":-0.53,"Deltam.z..Da.":-0.00047,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2021664.375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":10.05367,"Ion.Inject.Time..ms.":22,"RT..min.":4.9369,"First.Scan":1722,"Last.Scan":1722,"Master.Scan.s.":1721,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_02.raw","File.ID":"F20","Quan.Info":"","Peptides.Matched":3684,"XCorr":6.39,"X..Protein.Groups":1,"Percolator.q.Value":0.00036,"Percolator.PEP":5.398e-17,"Percolator.SVMScore":7.657,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Q4)","Search.ID":"Q","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KDELTLEGIK","Annotated.Sequence":"[K].KDELTLEGIK.[Q]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5104,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":573.32422,"Contaminant":"False","Aligned.m.z..Da.":573.32427,"MH...Da.":1145.64116,"Theo..MH...Da.":1145.64123,"DeltaM..ppm.":-0.06,"Deltam.z..Da.":-0.00003,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2005258.375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":12.42063,"Ion.Inject.Time..ms.":22,"RT..min.":10.251,"First.Scan":8606,"Last.Scan":8606,"Master.Scan.s.":8598,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_02.raw","File.ID":"F20","Quan.Info":"","Peptides.Matched":307,"XCorr":3.84,"X..Protein.Groups":1,"Percolator.q.Value":0.00036,"Percolator.PEP":1.992e-08,"Percolator.SVMScore":3.28,"Precursor.Area":2546017.75,"Apex.RT..min.":10.26,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Q4)","Search.ID":"Q","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"APQALMLAPTR","Annotated.Sequence":"[K].APQALmLAPTR.[E]","Modifications":"M6(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7096,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":592.8269,"Contaminant":"False","Aligned.m.z..Da.":592.82695,"MH...Da.":1184.64652,"Theo..MH...Da.":1184.6456,"DeltaM..ppm.":0.78,"Deltam.z..Da.":0.00046,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2304188.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":10.41533,"Ion.Inject.Time..ms.":22,"RT..min.":10.1843,"First.Scan":8522,"Last.Scan":8522,"Master.Scan.s.":8520,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_02.raw","File.ID":"F20","Quan.Info":"","Peptides.Matched":384,"XCorr":3.03,"X..Protein.Groups":1,"Percolator.q.Value":0.00036,"Percolator.PEP":3.017e-06,"Percolator.SVMScore":2.272,"Precursor.Area":2233673.5,"Apex.RT..min.":10.2,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Q4)","Search.ID":"Q","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"AAEAGETGAATSATEGDNNNNTAAGDKK","Annotated.Sequence":"[K].AAEAGETGAATsATEGDNNNNTAAGDKK.[G]","Modifications":"S12(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0087,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":906.04965,"Contaminant":"False","Aligned.m.z..Da.":906.04969,"MH...Da.":2716.13439,"Theo..MH...Da.":2716.13298,"DeltaM..ppm.":0.52,"Deltam.z..Da.":0.00047,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3940657.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":4.09739,"Ion.Inject.Time..ms.":22,"RT..min.":5.0282,"First.Scan":1804,"Last.Scan":1804,"Master.Scan.s.":1799,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_02.raw","File.ID":"F20","Quan.Info":"","Peptides.Matched":2540,"XCorr":6.87,"X..Protein.Groups":1,"Percolator.q.Value":0.00036,"Percolator.PEP":6.305e-16,"Percolator.SVMScore":6.959,"ptmRS..Binomial.Peptide.Score":480.39,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(15): 1300.57; y1+-H(3) O(4) P(18): 1789.79; y1+-H(3) O(4) P(17): 1688.75; y2+-H(3) O(4) P(24): 1138.50; y2+-H(3) O(4) P(22): 1045.47; y2+-H(3) O(4) P(19): 930.92; y2+-H(3) O(4) P(18): 895.40; y2+-H(3) O(4) P(17): 844.88","ptmRS..Best.Site.Probabilities":"S12(Phospho): 99.99","ptmRS..Phospho.Site.Probabilities":"T(7): 0.0; T(11): 0.0; S(12): 100.0; T(14): 0.0; T(22): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (R4)","Search.ID":"R","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LQETNPEEVPKFEK","Annotated.Sequence":"[K].LQETNPEEVPKFEK.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.582,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":563.29048,"Contaminant":"False","Aligned.m.z..Da.":563.29046,"MH...Da.":1687.85689,"Theo..MH...Da.":1687.85374,"DeltaM..ppm.":1.87,"Deltam.z..Da.":0.00105,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1901858.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":63.79973,"Ion.Inject.Time..ms.":22,"RT..min.":9.3209,"First.Scan":7447,"Last.Scan":7447,"Master.Scan.s.":7439,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_01.raw","File.ID":"F19","Quan.Info":"","Peptides.Matched":878,"XCorr":1.89,"X..Protein.Groups":1,"Percolator.q.Value":0.00036,"Percolator.PEP":0.00014,"Percolator.SVMScore":1.399,"Precursor.Area":1255273.25,"Apex.RT..min.":9.32,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (R4)","Search.ID":"R","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DFRHSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[K].DFRHSDsYsENETNHTNVPISSTGGTNNK.[T]","Modifications":"S7(Phospho); S9(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":1,"Charge":4,"Original.Precursor.Charge":4,"DeltaScore":0.0111,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":842.84191,"Contaminant":"False","Aligned.m.z..Da.":842.84195,"MH...Da.":3368.34581,"Theo..MH...Da.":3368.34888,"DeltaM..ppm.":-0.91,"Deltam.z..Da.":-0.00077,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":705206.3125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":14.43367,"Ion.Inject.Time..ms.":22,"RT..min.":8.6579,"First.Scan":6691,"Last.Scan":6691,"Master.Scan.s.":6685,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_01.raw","File.ID":"F19","Quan.Info":"","Peptides.Matched":917,"XCorr":6.31,"X..Protein.Groups":1,"Percolator.q.Value":0.00036,"Percolator.PEP":3.91e-08,"Percolator.SVMScore":2.936,"ptmRS..Binomial.Peptide.Score":330.21,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"b2+-H(3) O(4) P(8): 535.69; b2+- -2H(3) O(4) P -2H(3) O(4) P(16): 942.88; b2+- -2H(3) O(4) P -2H(3) O(4) P(17): 999.90; b2+- -2H(3) O(4) P -2H(3) O(4) P(18): 1049.44; b2+- -2H(3) O(4) P -2H(3) O(4) P(20): 1154.50","ptmRS..Best.Site.Probabilities":"S5(Phospho): 99.98; S7(Phospho): 98.49","ptmRS..Phospho.Site.Probabilities":"S(5): 100.0; S(7): 98.5; Y(8): 0.0; S(9): 1.5; T(13): 0.0; T(16): 0.0; S(21): 0.0; S(22): 0.0; T(23): 0.0; T(26): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (R4)","Search.ID":"R","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"APQALMLAPTR","Annotated.Sequence":"[K].APQALmLAPTR.[E]","Modifications":"M6(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7128,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":592.82612,"Contaminant":"False","Aligned.m.z..Da.":592.82616,"MH...Da.":1184.64498,"Theo..MH...Da.":1184.6456,"DeltaM..ppm.":-0.53,"Deltam.z..Da.":-0.00031,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2215809.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":16.44023,"Ion.Inject.Time..ms.":22,"RT..min.":10.176,"First.Scan":8461,"Last.Scan":8461,"Master.Scan.s.":8453,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_01.raw","File.ID":"F19","Quan.Info":"","Peptides.Matched":327,"XCorr":2.96,"X..Protein.Groups":1,"Percolator.q.Value":0.00036,"Percolator.PEP":3.722e-06,"Percolator.SVMScore":2.078,"Precursor.Area":2334760.5,"Apex.RT..min.":10.19,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (R4)","Search.ID":"R","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KDELTLEGIK","Annotated.Sequence":"[K].KDELTLEGIK.[Q]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5101,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":573.32429,"Contaminant":"False","Aligned.m.z..Da.":573.32433,"MH...Da.":1145.64131,"Theo..MH...Da.":1145.64123,"DeltaM..ppm.":0.07,"Deltam.z..Da.":0.00004,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2765010.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":9.94752,"Ion.Inject.Time..ms.":22,"RT..min.":10.2379,"First.Scan":8535,"Last.Scan":8535,"Master.Scan.s.":8531,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_01.raw","File.ID":"F19","Quan.Info":"","Peptides.Matched":307,"XCorr":3.96,"X..Protein.Groups":1,"Percolator.q.Value":0.00036,"Percolator.PEP":1.408e-08,"Percolator.SVMScore":3.129,"Precursor.Area":2138422.25,"Apex.RT..min.":10.25,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (R4)","Search.ID":"R","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"IQDPQMTGYVSTR","Annotated.Sequence":"[R].IQDPQMTGyVSTR.[Y]","Modifications":"Y9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P32485","Master.Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","Protein.Accessions":"P32485","Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1191,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":788.34532,"Contaminant":"False","Aligned.m.z..Da.":788.34529,"MH...Da.":1575.68337,"Theo..MH...Da.":1575.68728,"DeltaM..ppm.":-2.48,"Deltam.z..Da.":-0.00196,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3848635,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":9.5731,"First.Scan":7741,"Last.Scan":7741,"Master.Scan.s.":7738,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_01.raw","File.ID":"F19","Quan.Info":"","Peptides.Matched":1099,"XCorr":3.19,"X..Protein.Groups":1,"Percolator.q.Value":0.00036,"Percolator.PEP":3.136e-06,"Percolator.SVMScore":2.11,"Precursor.Area":3678908.5,"Apex.RT..min.":9.58,"ptmRS..Binomial.Peptide.Score":273.11,"ptmRS..Isoform.Confidence.Probability":0.333,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(11): 1236.57; y1+-H(3) O(4) P(10): 1121.54","ptmRS..Best.Site.Probabilities":"T7(Phospho): 33.28; Y9(Phospho): 33.28; S11(Phospho): 33.28","ptmRS..Phospho.Site.Probabilities":"T(7): 33.3; Y(9): 33.3; S(11): 33.3; T(12): 0.2"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (R4)","Search.ID":"R","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"AGNFALHPEVVR","Annotated.Sequence":"[R].AGNFALHPEVVR.[E]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q03558","Master.Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","Protein.Accessions":"Q03558","Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.5942,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":437.23893,"Contaminant":"False","Aligned.m.z..Da.":437.23894,"MH...Da.":1309.70225,"Theo..MH...Da.":1309.70114,"DeltaM..ppm.":0.84,"Deltam.z..Da.":0.00037,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1575892.375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":14.91664,"Ion.Inject.Time..ms.":22,"RT..min.":10.0276,"First.Scan":8283,"Last.Scan":8283,"Master.Scan.s.":8271,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_01.raw","File.ID":"F19","Quan.Info":"","Peptides.Matched":400,"XCorr":3.13,"X..Protein.Groups":1,"Percolator.q.Value":0.00036,"Percolator.PEP":1.995e-06,"Percolator.SVMScore":2.195,"Precursor.Area":909179,"Apex.RT..min.":10.03,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (R4)","Search.ID":"R","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"AAEAGETGAATSATEGDNNNNTAAGDKK","Annotated.Sequence":"[K].AAEAGETGAATsATEGDNNNNTAAGDKK.[G]","Modifications":"S12(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0503,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":906.05007,"Contaminant":"False","Aligned.m.z..Da.":906.05006,"MH...Da.":2716.13568,"Theo..MH...Da.":2716.13298,"DeltaM..ppm.":0.99,"Deltam.z..Da.":0.0009,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3653360.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":2.77464,"Ion.Inject.Time..ms.":22,"RT..min.":5.0473,"First.Scan":1880,"Last.Scan":1880,"Master.Scan.s.":1875,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_01.raw","File.ID":"F19","Quan.Info":"","Peptides.Matched":2509,"XCorr":7.15,"X..Protein.Groups":1,"Percolator.q.Value":0.00036,"Percolator.PEP":8.788e-17,"Percolator.SVMScore":6.709,"ptmRS..Binomial.Peptide.Score":544.16,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(15): 1300.57; y1+-H(3) O(4) P(18): 1789.79; y1+-H(3) O(4) P(17): 1688.75; y2+-H(3) O(4) P(21): 994.95; y2+-H(3) O(4) P(19): 930.92; y2+-H(3) O(4) P(18): 895.40; y2+-H(3) O(4) P(17): 844.88","ptmRS..Best.Site.Probabilities":"S12(Phospho): 99.99","ptmRS..Phospho.Site.Probabilities":"T(7): 0.0; T(11): 0.0; S(12): 100.0; T(14): 0.0; T(22): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (R4)","Search.ID":"R","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"HSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[R].HSDSYsENETNHTNVPISSTGGTNNK.[T]","Modifications":"S6(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0081,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":957.40101,"Contaminant":"False","Aligned.m.z..Da.":957.40106,"MH...Da.":2870.18849,"Theo..MH...Da.":2870.18608,"DeltaM..ppm.":0.84,"Deltam.z..Da.":0.00081,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2830162.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":7.6479,"First.Scan":5415,"Last.Scan":5415,"Master.Scan.s.":5411,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_01.raw","File.ID":"F19","Quan.Info":"","Peptides.Matched":2545,"XCorr":6.16,"X..Protein.Groups":1,"Percolator.q.Value":0.00036,"Percolator.PEP":4.241e-11,"Percolator.SVMScore":4.223,"Precursor.Area":9625762,"Apex.RT..min.":7.66,"ptmRS..Binomial.Peptide.Score":595.57,"ptmRS..Isoform.Confidence.Probability":0.006,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(4): 409.15; b1+-H(3) O(4) P(5): 572.21; b1+-H(3) O(4) P(6): 659.24; b1+-H(3) O(4) P(9): 1031.37; b1+-H(3) O(4) P(10): 1132.42; b1+-H(3) O(4) P(11): 1246.46; b1+-H(3) O(4) P(12): 1383.52; b1+-H(3) O(4) P(15): 1697.68; b2+-H(3) O(4) P(12): 692.26; b2+-H(3) O(4) P(13): 742.79; b2+-H(3) O(4) P(14): 799.81; b2+-H(3) O(4) P(15): 849.34; y2+-H(3) O(4) P(25): 1318.08; y2+-H(3) O(4) P(24): 1274.56; y2+-H(3) O(4) P(23): 1217.05","ptmRS..Best.Site.Probabilities":"S4(Phospho): 99.4","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(4): 99.4; Y(5): 0.0; S(6): 0.6; T(10): 0.0; T(13): 0.0; S(18): 0.0; S(19): 0.0; T(20): 0.0; T(23): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (R4)","Search.ID":"R","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"HSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[R].HSDSySENETNHTNVPISSTGGTNNK.[T]","Modifications":"Y5(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":0,"Charge":4,"Original.Precursor.Charge":4,"DeltaScore":0.0033,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":718.30254,"Contaminant":"False","Aligned.m.z..Da.":718.30258,"MH...Da.":2870.18836,"Theo..MH...Da.":2870.18608,"DeltaM..ppm.":0.79,"Deltam.z..Da.":0.00057,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2419509.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":3.46852,"Ion.Inject.Time..ms.":22,"RT..min.":7.6502,"First.Scan":5419,"Last.Scan":5419,"Master.Scan.s.":5411,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_01.raw","File.ID":"F19","Quan.Info":"","Peptides.Matched":2539,"XCorr":5.98,"X..Protein.Groups":1,"Percolator.q.Value":0.00036,"Percolator.PEP":2.707e-10,"Percolator.SVMScore":3.873,"Precursor.Area":6688940,"Apex.RT..min.":7.66,"ptmRS..Binomial.Peptide.Score":286.09,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(9): 1031.37; b1+-H(3) O(4) P(10): 1132.42; b1+-H(3) O(4) P(11): 1246.46; b2+-H(3) O(4) P(7): 394.65; b2+-H(3) O(4) P(13): 742.79; b2+-H(3) O(4) P(14): 799.81; b2+-H(3) O(4) P(15): 849.34; b2+-H(3) O(4) P(17): 954.41; b1+(4): 427.16","ptmRS..Best.Site.Probabilities":"S6(Phospho): 99.33","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(4): 0.7; Y(5): 0.0; S(6): 99.3; T(10): 0.0; T(13): 0.0; S(18): 0.0; S(19): 0.0; T(20): 0.0; T(23): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (R4)","Search.ID":"R","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"IQDPQMTGYVSTR","Annotated.Sequence":"[R].IQDPQmTGyVSTR.[Y]","Modifications":"M6(Oxidation); Y9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P32485","Master.Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","Protein.Accessions":"P32485","Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1318,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":796.34548,"Contaminant":"False","Aligned.m.z..Da.":796.34552,"MH...Da.":1591.68369,"Theo..MH...Da.":1591.6822,"DeltaM..ppm.":0.94,"Deltam.z..Da.":0.00074,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2207360.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":7.6579,"First.Scan":5430,"Last.Scan":5430,"Master.Scan.s.":5424,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_01.raw","File.ID":"F19","Quan.Info":"","Peptides.Matched":1114,"XCorr":3.11,"X..Protein.Groups":1,"Percolator.q.Value":0.00036,"Percolator.PEP":8.119e-07,"Percolator.SVMScore":2.365,"Precursor.Area":4999126,"Apex.RT..min.":7.69,"ptmRS..Binomial.Peptide.Score":299.6,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+(6): 762.32; y1+(5): 705.30","ptmRS..Best.Site.Probabilities":"Y9(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"T(7): 0.0; Y(9): 100.0; S(11): 0.0; T(12): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (R4)","Search.ID":"R","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"IQDPQMTGYVSTR","Annotated.Sequence":"[R].IQDPQmtGYVsTR.[Y]","Modifications":"M6(Oxidation); T7(Phospho); S11(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P32485","Master.Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","Protein.Accessions":"P32485","Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.0547,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":836.32904,"Contaminant":"False","Aligned.m.z..Da.":836.32909,"MH...Da.":1671.65081,"Theo..MH...Da.":1671.64853,"DeltaM..ppm.":1.36,"Deltam.z..Da.":0.00114,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2249475.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":11.46216,"Ion.Inject.Time..ms.":22,"RT..min.":7.7675,"First.Scan":5591,"Last.Scan":5591,"Master.Scan.s.":5580,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_01.raw","File.ID":"F19","Quan.Info":"","Peptides.Matched":713,"XCorr":2.74,"X..Protein.Groups":1,"Percolator.q.Value":0.00036,"Percolator.PEP":0.00012,"Percolator.SVMScore":1.431,"Precursor.Area":1841725.5,"Apex.RT..min.":7.75,"ptmRS..Binomial.Peptide.Score":315.58,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(11): 1332.53; y1+-H(3) O(4) P(10): 1217.50; y1+-H(3) O(4) P(8): 992.39; y1+-H(3) O(4) P(7): 845.36; y2+-H(3) O(4) P(11): 666.77; y2+-H(3) O(4) P(10): 609.25; y1+(4): 462.27; y1+(3): 363.20","ptmRS..Best.Site.Probabilities":"T7(Phospho): 100; Y9(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"T(7): 100.0; Y(9): 100.0; S(11): 0.0; T(12): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (R4)","Search.ID":"R","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LQETNPEEVPK","Annotated.Sequence":"[K].LQETNPEEVPK.[F]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5576,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":642.32796,"Contaminant":"False","Aligned.m.z..Da.":642.32799,"MH...Da.":1283.64865,"Theo..MH...Da.":1283.64777,"DeltaM..ppm.":0.68,"Deltam.z..Da.":0.00044,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2981399.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":2.01036,"Ion.Inject.Time..ms.":22,"RT..min.":7.6388,"First.Scan":5401,"Last.Scan":5401,"Master.Scan.s.":5398,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_01.raw","File.ID":"F19","Quan.Info":"","Peptides.Matched":573,"XCorr":2.78,"X..Protein.Groups":1,"Percolator.q.Value":0.00036,"Percolator.PEP":0.00003,"Percolator.SVMScore":1.662,"Precursor.Area":6344100.5,"Apex.RT..min.":7.66,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (R4)","Search.ID":"R","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"HSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[R].HSDsYsENETNHTNVPISSTGGTNNK.[T]","Modifications":"S4(Phospho); S6(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.1515,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":984.05625,"Contaminant":"False","Aligned.m.z..Da.":984.05634,"MH...Da.":2950.15419,"Theo..MH...Da.":2950.15241,"DeltaM..ppm.":0.61,"Deltam.z..Da.":0.0006,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2384647.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":8.0331,"First.Scan":5950,"Last.Scan":5950,"Master.Scan.s.":5944,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_01.raw","File.ID":"F19","Quan.Info":"","Peptides.Matched":1153,"XCorr":6.27,"X..Protein.Groups":1,"Percolator.q.Value":0.00036,"Percolator.PEP":2.526e-10,"Percolator.SVMScore":3.886,"Precursor.Area":3022073.5,"Apex.RT..min.":8.04,"ptmRS..Binomial.Peptide.Score":633.35,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(6): 739.21; y2+-H(3) O(4) P(25): 1358.06; y2+-H(3) O(4) P(22): 1173.53; y2+-H(3) O(4) P(21): 1092.00; b1+- -2H(3) O(4) P -2H(3) O(4) P(6): 641.23; b1+- -2H(3) O(4) P -2H(3) O(4) P(11): 1228.45; b2+- -2H(3) O(4) P -2H(3) O(4) P(13): 733.78; b2+- -2H(3) O(4) P -2H(3) O(4) P(14): 790.80; b2+- -2H(3) O(4) P -2H(3) O(4) P(15): 840.34","ptmRS..Best.Site.Probabilities":"S4(Phospho): 100; S6(Phospho): 99.99","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(4): 100.0; Y(5): 0.0; S(6): 100.0; T(10): 0.0; T(13): 0.0; S(18): 0.0; S(19): 0.0; T(20): 0.0; T(23): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (R4)","Search.ID":"R","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":5,"DeltaScore":0.0586,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40308,"Contaminant":"False","Aligned.m.z..Da.":967.40321,"MH...Da.":2900.19468,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":-1.6,"Deltam.z..Da.":-0.00155,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":22101948,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":4.01829,"Ion.Inject.Time..ms.":7.906,"RT..min.":15.1013,"First.Scan":14729,"Last.Scan":14729,"Master.Scan.s.":14722,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_01.raw","File.ID":"F19","Quan.Info":"","Peptides.Matched":2261,"XCorr":7.51,"X..Protein.Groups":1,"Percolator.q.Value":0.00036,"Percolator.PEP":7.967e-16,"Percolator.SVMScore":6.286,"Precursor.Area":22237282,"Apex.RT..min.":15.09,"ptmRS..Binomial.Peptide.Score":906.55,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(13): 1413.58; b1+-H(3) O(4) P(14): 1528.61; b1+-H(3) O(4) P(15): 1642.65; b1+-H(3) O(4) P(16): 1771.69; b1+-H(3) O(4) P(19): 2141.88; y1+-H(3) O(4) P(15): 1750.78; y1+-H(3) O(4) P(14): 1621.73; y1+-H(3) O(4) P(13): 1458.67; b2+-H(3) O(4) P(16): 886.35; b2+-H(3) O(4) P(17): 950.38; b2+-H(3) O(4) P(18): 1014.90; y2+-H(3) O(4) P(14): 811.37; b1+(12): 1344.56; y1+(13): 1556.65","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (R4)","Search.ID":"R","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FFISNPDLVDRLEK","Annotated.Sequence":"[R].FFISNPDLVDRLEK.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q03558","Master.Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","Protein.Accessions":"Q03558","Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.659,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":564.96988,"Contaminant":"False","Aligned.m.z..Da.":564.96992,"MH...Da.":1692.8951,"Theo..MH...Da.":1692.89555,"DeltaM..ppm.":-0.27,"Deltam.z..Da.":-0.00015,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":318593.53125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":29.0122,"Ion.Inject.Time..ms.":22,"RT..min.":15.3193,"First.Scan":14987,"Last.Scan":14987,"Master.Scan.s.":14978,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_01.raw","File.ID":"F19","Quan.Info":"","Peptides.Matched":560,"XCorr":2.17,"X..Protein.Groups":1,"Percolator.q.Value":0.00036,"Percolator.PEP":0.00007,"Percolator.SVMScore":1.533,"Precursor.Area":251189.4375,"Apex.RT..min.":15.31,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (R4)","Search.ID":"R","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":5,"DeltaScore":0.1026,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40601,"Contaminant":"False","Aligned.m.z..Da.":967.40609,"MH...Da.":2900.20347,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":1.43,"Deltam.z..Da.":0.00138,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1627129.875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":17.74039,"Ion.Inject.Time..ms.":22,"RT..min.":15.2354,"First.Scan":14891,"Last.Scan":14891,"Master.Scan.s.":14889,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_01.raw","File.ID":"F19","Quan.Info":"","Peptides.Matched":2586,"XCorr":6.92,"X..Protein.Groups":1,"Percolator.q.Value":0.00036,"Percolator.PEP":2.4e-13,"Percolator.SVMScore":5.198,"Precursor.Area":22237282,"Apex.RT..min.":15.09,"ptmRS..Binomial.Peptide.Score":670.42,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(13): 1413.58; b1+-H(3) O(4) P(14): 1528.61; b1+-H(3) O(4) P(16): 1771.69; y1+-H(3) O(4) P(14): 1621.73; y1+-H(3) O(4) P(13): 1458.67; b2+-H(3) O(4) P(16): 886.35; b2+-H(3) O(4) P(18): 1014.90; y2+-H(3) O(4) P(14): 811.37; b1+(12): 1344.56; y1+(13): 1556.65","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (R4)","Search.ID":"R","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FTVSAIYSDLPQQER","Annotated.Sequence":"[K].FTVSAIYSDLPQQER.[D]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6831,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":877.44058,"Contaminant":"False","Aligned.m.z..Da.":877.44072,"MH...Da.":1753.87389,"Theo..MH...Da.":1753.87554,"DeltaM..ppm.":-0.94,"Deltam.z..Da.":-0.00083,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":508435.34375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":37.13152,"Ion.Inject.Time..ms.":22,"RT..min.":15.0051,"First.Scan":14609,"Last.Scan":14609,"Master.Scan.s.":14605,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_01.raw","File.ID":"F19","Quan.Info":"","Peptides.Matched":1155,"XCorr":3.85,"X..Protein.Groups":1,"Percolator.q.Value":0.00036,"Percolator.PEP":5.042e-09,"Percolator.SVMScore":3.322,"Precursor.Area":667729.75,"Apex.RT..min.":15.02,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (R4)","Search.ID":"R","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"GVFGYGFEEPSAIQQR","Annotated.Sequence":"[R].GVFGYGFEEPSAIQQR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6727,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":892.93495,"Contaminant":"False","Aligned.m.z..Da.":892.93509,"MH...Da.":1784.86263,"Theo..MH...Da.":1784.86022,"DeltaM..ppm.":1.35,"Deltam.z..Da.":0.0012,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":819787.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":15.0045,"First.Scan":14608,"Last.Scan":14608,"Master.Scan.s.":14605,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_01.raw","File.ID":"F19","Quan.Info":"","Peptides.Matched":1390,"XCorr":3.88,"X..Protein.Groups":1,"Percolator.q.Value":0.00036,"Percolator.PEP":1.391e-10,"Percolator.SVMScore":3.999,"Precursor.Area":934604.8125,"Apex.RT..min.":15.02,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (R4)","Search.ID":"R","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].msEGITDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S2(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.1147,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":731.31496,"Contaminant":"False","Aligned.m.z..Da.":731.31491,"MH...Da.":2191.93033,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":1.14,"Deltam.z..Da.":0.00083,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":606985.375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":12.00897,"Ion.Inject.Time..ms.":22,"RT..min.":15.7615,"First.Scan":15509,"Last.Scan":15509,"Master.Scan.s.":15506,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_01.raw","File.ID":"F19","Quan.Info":"","Peptides.Matched":2083,"XCorr":3.4,"X..Protein.Groups":1,"Percolator.q.Value":0.00036,"Percolator.PEP":0.00004,"Percolator.SVMScore":1.623,"Precursor.Area":1946641.5,"Apex.RT..min.":15.8,"ptmRS..Binomial.Peptide.Score":439.32,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(2): 112.04; b1+-H(3) O(4) P(3): 241.08; b1+-H(3) O(4) P(4): 298.10; b1+-H(3) O(4) P(5): 411.19; b1+-H(3) O(4) P(6): 512.24; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+-H(3) O(4) P(9): 869.39; b1+-H(3) O(4) P(10): 998.43","ptmRS..Best.Site.Probabilities":"S2(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 100.0; T(6): 0.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (R4)","Search.ID":"R","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].msEGITDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S2(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":3,"DeltaScore":0.1583,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1096.46643,"Contaminant":"False","Aligned.m.z..Da.":1096.46635,"MH...Da.":2191.92559,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":-1.03,"Deltam.z..Da.":-0.00113,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":13774242,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":21.88993,"Ion.Inject.Time..ms.":22,"RT..min.":15.7986,"First.Scan":15556,"Last.Scan":15556,"Master.Scan.s.":15545,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_01.raw","File.ID":"F19","Quan.Info":"","Peptides.Matched":1883,"XCorr":3.79,"X..Protein.Groups":1,"Percolator.q.Value":0.00036,"Percolator.PEP":4.38e-06,"Percolator.SVMScore":2.047,"Precursor.Area":11819790,"Apex.RT..min.":15.8,"ptmRS..Binomial.Peptide.Score":369.52,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(2): 112.04; b1+-H(3) O(4) P(3): 241.08; b1+-H(3) O(4) P(4): 298.10; b1+-H(3) O(4) P(5): 411.19; b1+-H(3) O(4) P(6): 512.24; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+-H(3) O(4) P(9): 869.39; b1+-H(3) O(4) P(10): 998.43; b1+-H(3) O(4) P(16): 1669.76","ptmRS..Best.Site.Probabilities":"S2(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 100.0; T(6): 0.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (R4)","Search.ID":"R","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":4,"DeltaScore":0.0141,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.4067,"Contaminant":"False","Aligned.m.z..Da.":967.40672,"MH...Da.":2900.20553,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":2.14,"Deltam.z..Da.":0.00207,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":975745.5625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":19.53768,"Ion.Inject.Time..ms.":22,"RT..min.":15.4476,"First.Scan":15144,"Last.Scan":15144,"Master.Scan.s.":15134,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_01.raw","File.ID":"F19","Quan.Info":"","Peptides.Matched":2575,"XCorr":4.95,"X..Protein.Groups":1,"Percolator.q.Value":0.00036,"Percolator.PEP":1.382e-09,"Percolator.SVMScore":3.566,"Precursor.Area":22237282,"Apex.RT..min.":15.09,"ptmRS..Binomial.Peptide.Score":331.07,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y2+-H(3) O(4) P(22): 1268.56; b1+(12): 1344.56","ptmRS..Best.Site.Probabilities":"S13(Phospho): 99.99","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (R4)","Search.ID":"R","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ILISTDLLAR","Annotated.Sequence":"[R].ILISTDLLAR.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.524,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":557.84558,"Contaminant":"False","Aligned.m.z..Da.":557.84556,"MH...Da.":1114.68388,"Theo..MH...Da.":1114.68304,"DeltaM..ppm.":0.76,"Deltam.z..Da.":0.00042,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1339394.125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":19.32347,"Ion.Inject.Time..ms.":22,"RT..min.":15.6002,"First.Scan":15315,"Last.Scan":15315,"Master.Scan.s.":15309,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_01.raw","File.ID":"F19","Quan.Info":"","Peptides.Matched":124,"XCorr":2.71,"X..Protein.Groups":1,"Percolator.q.Value":0.00036,"Percolator.PEP":0.00008,"Percolator.SVMScore":1.495,"Precursor.Area":1218944.625,"Apex.RT..min.":15.61,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (R4)","Search.ID":"R","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].mSEGItDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); T6(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1092,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1096.46979,"Contaminant":"False","Aligned.m.z..Da.":1096.46978,"MH...Da.":2191.9323,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":2.03,"Deltam.z..Da.":0.00223,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":230188.89062,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":15.5331,"First.Scan":15248,"Last.Scan":15248,"Master.Scan.s.":15238,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_01.raw","File.ID":"F19","Quan.Info":"","Peptides.Matched":2162,"XCorr":4.67,"X..Protein.Groups":1,"Percolator.q.Value":0.00036,"Percolator.PEP":6.383e-11,"Percolator.SVMScore":4.146,"Precursor.Area":196509.90625,"Apex.RT..min.":15.53,"ptmRS..Binomial.Peptide.Score":478.66,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(6): 512.24; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+-H(3) O(4) P(9): 869.39; b1+-H(3) O(4) P(10): 998.43; y2+-H(3) O(4) P(16): 918.44; b1+(3): 259.09; b1+(4): 316.11; b1+(5): 429.20","ptmRS..Best.Site.Probabilities":"T6(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; T(6): 100.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (R4)","Search.ID":"R","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0562,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40758,"Contaminant":"False","Aligned.m.z..Da.":967.40757,"MH...Da.":2900.2082,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":3.06,"Deltam.z..Da.":0.00296,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":498525.78125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":6.1911,"Ion.Inject.Time..ms.":22,"RT..min.":15.5379,"First.Scan":15252,"Last.Scan":15252,"Master.Scan.s.":15251,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_01.raw","File.ID":"F19","Quan.Info":"","Peptides.Matched":2633,"XCorr":4.45,"X..Protein.Groups":1,"Percolator.q.Value":0.00036,"Percolator.PEP":1.403e-08,"Percolator.SVMScore":3.129,"ptmRS..Binomial.Peptide.Score":411.9,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(13): 1458.67; b2+-H(3) O(4) P(18): 1014.90; b1+(12): 1344.56","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (R4)","Search.ID":"R","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].mSEGITDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7105,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1056.48241,"Contaminant":"False","Aligned.m.z..Da.":1056.48266,"MH...Da.":2111.95755,"Theo..MH...Da.":2111.96151,"DeltaM..ppm.":-1.88,"Deltam.z..Da.":-0.00198,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":206201.29688,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":92.45267,"Ion.Inject.Time..ms.":22,"RT..min.":14.6465,"First.Scan":14209,"Last.Scan":14209,"Master.Scan.s.":14208,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_01.raw","File.ID":"F19","Quan.Info":"","Peptides.Matched":2204,"XCorr":4.18,"X..Protein.Groups":1,"Percolator.q.Value":0.00036,"Percolator.PEP":2.127e-10,"Percolator.SVMScore":3.919,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (R4)","Search.ID":"R","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FDDMELDENLLR","Annotated.Sequence":"[K].FDDmELDENLLR.[G]","Modifications":"M4(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5871,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":763.34278,"Contaminant":"False","Aligned.m.z..Da.":763.34298,"MH...Da.":1525.6783,"Theo..MH...Da.":1525.6839,"DeltaM..ppm.":-3.67,"Deltam.z..Da.":-0.0028,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":794809.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":72.95291,"Ion.Inject.Time..ms.":22,"RT..min.":14.4634,"First.Scan":14031,"Last.Scan":14031,"Master.Scan.s.":14028,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_01.raw","File.ID":"F19","Quan.Info":"","Peptides.Matched":1022,"XCorr":2.64,"X..Protein.Groups":1,"Percolator.q.Value":0.00036,"Percolator.PEP":5.253e-06,"Percolator.SVMScore":2.013,"Precursor.Area":1065919.75,"Apex.RT..min.":14.48,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (R4)","Search.ID":"R","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0546,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40384,"Contaminant":"False","Aligned.m.z..Da.":967.40365,"MH...Da.":2900.19697,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":-0.81,"Deltam.z..Da.":-0.00079,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":157069.17188,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":21.23838,"Ion.Inject.Time..ms.":22,"RT..min.":16.5786,"First.Scan":16485,"Last.Scan":16485,"Master.Scan.s.":16483,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_01.raw","File.ID":"F19","Quan.Info":"","Peptides.Matched":2400,"XCorr":4.58,"X..Protein.Groups":1,"Percolator.q.Value":0.00036,"Percolator.PEP":4.385e-07,"Percolator.SVMScore":2.481,"Precursor.Area":456655.28125,"Apex.RT..min.":16.65,"ptmRS..Binomial.Peptide.Score":363.14,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b2+-H(3) O(4) P(17): 950.38; b1+(12): 1344.56","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (R4)","Search.ID":"R","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0828,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40411,"Contaminant":"False","Aligned.m.z..Da.":967.40398,"MH...Da.":2900.19779,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":-0.53,"Deltam.z..Da.":-0.00051,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":488739.46875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":21.63047,"Ion.Inject.Time..ms.":22,"RT..min.":16.0687,"First.Scan":15884,"Last.Scan":15884,"Master.Scan.s.":15883,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_01.raw","File.ID":"F19","Quan.Info":"","Peptides.Matched":2423,"XCorr":4.47,"X..Protein.Groups":1,"Percolator.q.Value":0.00036,"Percolator.PEP":2.931e-09,"Percolator.SVMScore":3.424,"Precursor.Area":503346.25,"Apex.RT..min.":16.06,"ptmRS..Binomial.Peptide.Score":410.25,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(14): 1621.73; y2+-H(3) O(4) P(14): 811.37; b1+(12): 1344.56","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (R4)","Search.ID":"R","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].msEGITDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S2(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1199,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1096.47097,"Contaminant":"False","Aligned.m.z..Da.":1096.47082,"MH...Da.":2191.93467,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":3.11,"Deltam.z..Da.":0.00341,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":334336.1875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":51.80921,"Ion.Inject.Time..ms.":22,"RT..min.":16.0724,"First.Scan":15890,"Last.Scan":15890,"Master.Scan.s.":15883,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_01.raw","File.ID":"F19","Quan.Info":"","Peptides.Matched":2213,"XCorr":3.42,"X..Protein.Groups":1,"Percolator.q.Value":0.00036,"Percolator.PEP":3.34e-07,"Percolator.SVMScore":2.532,"ptmRS..Binomial.Peptide.Score":458.93,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(2): 112.04; b1+-H(3) O(4) P(3): 241.08; b1+-H(3) O(4) P(4): 298.10; b1+-H(3) O(4) P(5): 411.19; b1+-H(3) O(4) P(6): 512.24; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+-H(3) O(4) P(9): 869.39; b1+-H(3) O(4) P(10): 998.43; b1+(4): 396.08; y1+(14): 1683.77","ptmRS..Best.Site.Probabilities":"S2(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 100.0; T(6): 0.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (R4)","Search.ID":"R","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FDDMELDENLLR","Annotated.Sequence":"[K].FDDMELDENLLR.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5677,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":755.34783,"Contaminant":"False","Aligned.m.z..Da.":755.34773,"MH...Da.":1509.68838,"Theo..MH...Da.":1509.68898,"DeltaM..ppm.":-0.4,"Deltam.z..Da.":-0.0003,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":548842.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":41.29422,"Ion.Inject.Time..ms.":22,"RT..min.":16.0284,"First.Scan":15835,"Last.Scan":15835,"Master.Scan.s.":15831,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_01.raw","File.ID":"F19","Quan.Info":"","Peptides.Matched":1061,"XCorr":3.1,"X..Protein.Groups":1,"Percolator.q.Value":0.00036,"Percolator.PEP":3.326e-06,"Percolator.SVMScore":2.099,"Precursor.Area":566131.4375,"Apex.RT..min.":16.04,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (R4)","Search.ID":"R","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].msEGITDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S2(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1391,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1096.46657,"Contaminant":"False","Aligned.m.z..Da.":1096.46641,"MH...Da.":2191.92588,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":-0.9,"Deltam.z..Da.":-0.00098,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":175776.23438,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":30.73558,"Ion.Inject.Time..ms.":22,"RT..min.":16.1271,"First.Scan":15958,"Last.Scan":15958,"Master.Scan.s.":15948,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_01.raw","File.ID":"F19","Quan.Info":"","Peptides.Matched":1845,"XCorr":3.02,"X..Protein.Groups":1,"Percolator.q.Value":0.00036,"Percolator.PEP":0.00001,"Percolator.SVMScore":1.847,"Precursor.Area":11819790,"Apex.RT..min.":15.8,"ptmRS..Binomial.Peptide.Score":412.73,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(2): 112.04; b1+-H(3) O(4) P(3): 241.08; b1+-H(3) O(4) P(4): 298.10; b1+-H(3) O(4) P(5): 411.19; b1+-H(3) O(4) P(6): 512.24; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+-H(3) O(4) P(9): 869.39","ptmRS..Best.Site.Probabilities":"S2(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 100.0; T(6): 0.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (R4)","Search.ID":"R","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0647,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40274,"Contaminant":"False","Aligned.m.z..Da.":967.40258,"MH...Da.":2900.19367,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":-1.95,"Deltam.z..Da.":-0.00189,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":110079.89062,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":17.0718,"First.Scan":17119,"Last.Scan":17119,"Master.Scan.s.":17111,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_01.raw","File.ID":"F19","Quan.Info":"","Peptides.Matched":2203,"XCorr":4.48,"X..Protein.Groups":1,"Percolator.q.Value":0.00036,"Percolator.PEP":8.096e-08,"Percolator.SVMScore":2.799,"ptmRS..Binomial.Peptide.Score":385.65,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(14): 1621.73; b1+(12): 1344.56","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (R4)","Search.ID":"R","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEySDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); Y12(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":2,"DeltaScore":0.4121,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40516,"Contaminant":"False","Aligned.m.z..Da.":967.40508,"MH...Da.":2900.20092,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":0.55,"Deltam.z..Da.":0.00053,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":288445.5625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":20.08809,"Ion.Inject.Time..ms.":22,"RT..min.":17.368,"First.Scan":17500,"Last.Scan":17500,"Master.Scan.s.":17498,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_01.raw","File.ID":"F19","Quan.Info":"","Peptides.Matched":2507,"XCorr":1.99,"X..Protein.Groups":1,"Percolator.q.Value":0.00036,"Percolator.PEP":0.00003,"Percolator.SVMScore":1.669,"ptmRS..Binomial.Peptide.Score":259.58,"ptmRS..Isoform.Confidence.Probability":0.5,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"Y12(Phospho): 50; S13(Phospho): 50","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 50.0; S(13): 50.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (R4)","Search.ID":"R","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DIFSNDELLSDAYDAK","Annotated.Sequence":"[K].DIFSNDELLSDAYDAK.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.6583,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":605.94795,"Contaminant":"False","Aligned.m.z..Da.":605.94784,"MH...Da.":1815.82929,"Theo..MH...Da.":1815.82831,"DeltaM..ppm.":0.54,"Deltam.z..Da.":0.00033,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":416166.4375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":26.58219,"Ion.Inject.Time..ms.":22,"RT..min.":17.0601,"First.Scan":17103,"Last.Scan":17103,"Master.Scan.s.":17100,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_01.raw","File.ID":"F19","Quan.Info":"","Peptides.Matched":1560,"XCorr":2.4,"X..Protein.Groups":1,"Percolator.q.Value":0.00036,"Percolator.PEP":0.00012,"Percolator.SVMScore":1.42,"Precursor.Area":472220.53125,"Apex.RT..min.":17.07,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (R4)","Search.ID":"R","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DIFSNDELLSDAYDAK","Annotated.Sequence":"[K].DIFSNDELLSDAYDAK.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6954,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":908.41935,"Contaminant":"False","Aligned.m.z..Da.":908.4192,"MH...Da.":1815.83144,"Theo..MH...Da.":1815.82831,"DeltaM..ppm.":1.72,"Deltam.z..Da.":0.00156,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":532717.6875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":8.55717,"Ion.Inject.Time..ms.":22,"RT..min.":17.0517,"First.Scan":17092,"Last.Scan":17092,"Master.Scan.s.":17087,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_01.raw","File.ID":"F19","Quan.Info":"","Peptides.Matched":1697,"XCorr":4.17,"X..Protein.Groups":1,"Percolator.q.Value":0.00036,"Percolator.PEP":8.837e-11,"Percolator.SVMScore":4.084,"Precursor.Area":1947693.625,"Apex.RT..min.":17.08,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (R4)","Search.ID":"R","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"APQALMLAPTR","Annotated.Sequence":"[K].APQALMLAPTR.[E]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6318,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":584.82831,"Contaminant":"False","Aligned.m.z..Da.":584.82835,"MH...Da.":1168.64936,"Theo..MH...Da.":1168.65069,"DeltaM..ppm.":-1.14,"Deltam.z..Da.":-0.00067,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":803023.8125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":68.23862,"Ion.Inject.Time..ms.":22,"RT..min.":11.6224,"First.Scan":10292,"Last.Scan":10292,"Master.Scan.s.":10286,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_01.raw","File.ID":"F19","Quan.Info":"","Peptides.Matched":182,"XCorr":2.96,"X..Protein.Groups":1,"Percolator.q.Value":0.00036,"Percolator.PEP":6.804e-06,"Percolator.SVMScore":1.964,"Precursor.Area":613091.9375,"Apex.RT..min.":11.62,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (R4)","Search.ID":"R","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"APQALMLAPTR","Annotated.Sequence":"[K].APQALMLAPTR.[E]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":3,"DeltaScore":0.6524,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":584.82831,"Contaminant":"False","Aligned.m.z..Da.":584.82835,"MH...Da.":1168.64935,"Theo..MH...Da.":1168.65069,"DeltaM..ppm.":-1.15,"Deltam.z..Da.":-0.00067,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":803023.8125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":74.7778,"Ion.Inject.Time..ms.":22,"RT..min.":11.6236,"First.Scan":10294,"Last.Scan":10294,"Master.Scan.s.":10286,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_01.raw","File.ID":"F19","Quan.Info":"","Peptides.Matched":182,"XCorr":2.33,"X..Protein.Groups":1,"Percolator.q.Value":0.00036,"Percolator.PEP":7.425e-07,"Percolator.SVMScore":2.381,"Precursor.Area":613091.9375,"Apex.RT..min.":11.62,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (R4)","Search.ID":"R","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"TAVFYGGTPISK","Annotated.Sequence":"[K].TAVFYGGTPISK.[D]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5529,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":620.83159,"Contaminant":"False","Aligned.m.z..Da.":620.83166,"MH...Da.":1240.6559,"Theo..MH...Da.":1240.65721,"DeltaM..ppm.":-1.06,"Deltam.z..Da.":-0.00066,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":640512.8125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":52.78812,"Ion.Inject.Time..ms.":22,"RT..min.":11.1394,"First.Scan":9650,"Last.Scan":9650,"Master.Scan.s.":9649,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_01.raw","File.ID":"F19","Quan.Info":"","Peptides.Matched":408,"XCorr":2.55,"X..Protein.Groups":1,"Percolator.q.Value":0.00036,"Percolator.PEP":0.00009,"Percolator.SVMScore":1.479,"Precursor.Area":579283.8125,"Apex.RT..min.":11.13,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (R4)","Search.ID":"R","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MGSINNNFNTNNNSNTDLDRDWK","Annotated.Sequence":"[-].mGSINNNFNTNNNsNTDLDRDWK.[T]","Modifications":"N-Term(Prot)(Met-loss); S14(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P39517","Master.Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","Protein.Accessions":"P39517","Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0664,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":878.37096,"Contaminant":"False","Aligned.m.z..Da.":878.37105,"MH...Da.":2633.09834,"Theo..MH...Da.":2633.10123,"DeltaM..ppm.":-1.1,"Deltam.z..Da.":-0.00096,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":930298,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":11.2718,"First.Scan":9824,"Last.Scan":9824,"Master.Scan.s.":9818,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_01.raw","File.ID":"F19","Quan.Info":"","Peptides.Matched":2182,"XCorr":5.57,"X..Protein.Groups":1,"Percolator.q.Value":0.00036,"Percolator.PEP":4.824e-11,"Percolator.SVMScore":4.198,"Precursor.Area":3777489.25,"Apex.RT..min.":11.29,"ptmRS..Binomial.Peptide.Score":507.26,"ptmRS..Isoform.Confidence.Probability":0.994,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(12): 1459.66; y1+-H(3) O(4) P(11): 1345.61; y1+-H(3) O(4) P(10): 1231.57; y2+-H(3) O(4) P(19): 1139.50; y2+-H(3) O(4) P(17): 1025.45; y2+-H(3) O(4) P(16): 968.43; y2+-H(3) O(4) P(15): 894.90; y2+-H(3) O(4) P(14): 837.88; y2+-H(3) O(4) P(13): 787.35; y2+-H(3) O(4) P(12): 730.33; y2+-H(3) O(4) P(11): 673.31; y1+(9): 1162.55","ptmRS..Best.Site.Probabilities":"S14(Phospho): 99.45","ptmRS..Phospho.Site.Probabilities":"S(3): 0.0; T(10): 0.0; S(14): 99.5; T(16): 0.6"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (R4)","Search.ID":"R","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"SSQIHIENESTEDILK","Annotated.Sequence":"[R].SSQIHIENEStEDILK.[I]","Modifications":"T11(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0661,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":641.62985,"Contaminant":"False","Aligned.m.z..Da.":641.62992,"MH...Da.":1922.87502,"Theo..MH...Da.":1922.87429,"DeltaM..ppm.":0.38,"Deltam.z..Da.":0.00024,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":786291.0625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":11.09522,"Ion.Inject.Time..ms.":22,"RT..min.":11.2748,"First.Scan":9829,"Last.Scan":9829,"Master.Scan.s.":9818,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_01.raw","File.ID":"F19","Quan.Info":"","Peptides.Matched":1714,"XCorr":3.78,"X..Protein.Groups":1,"Percolator.q.Value":0.00036,"Percolator.PEP":7.636e-06,"Percolator.SVMScore":1.942,"Precursor.Area":480089.03125,"Apex.RT..min.":11.28,"ptmRS..Binomial.Peptide.Score":310.8,"ptmRS..Isoform.Confidence.Probability":0.5,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(7): 787.42; b2+-H(3) O(4) P(10): 554.26; y2+-H(3) O(4) P(7): 394.21","ptmRS..Best.Site.Probabilities":"S10(Phospho): 50; T11(Phospho): 50","ptmRS..Phospho.Site.Probabilities":"S(1): 0.0; S(2): 0.0; S(10): 50.0; T(11): 50.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (R4)","Search.ID":"R","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"TGTFSIAALQR","Annotated.Sequence":"[K].TGTFSIAALQR.[I]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7904,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":582.8226,"Contaminant":"False","Aligned.m.z..Da.":582.82278,"MH...Da.":1164.63794,"Theo..MH...Da.":1164.63715,"DeltaM..ppm.":0.68,"Deltam.z..Da.":0.00039,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1074205.375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":9.45199,"Ion.Inject.Time..ms.":22,"RT..min.":13.3631,"First.Scan":12643,"Last.Scan":12643,"Master.Scan.s.":12639,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_01.raw","File.ID":"F19","Quan.Info":"","Peptides.Matched":360,"XCorr":3.34,"X..Protein.Groups":1,"Percolator.q.Value":0.00036,"Percolator.PEP":4.569e-06,"Percolator.SVMScore":2.039,"Precursor.Area":1451202.625,"Apex.RT..min.":13.38,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (R4)","Search.ID":"R","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DFLLKPELSR","Annotated.Sequence":"[K].DFLLKPELSR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.5948,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":406.56765,"Contaminant":"False","Aligned.m.z..Da.":406.56776,"MH...Da.":1217.68841,"Theo..MH...Da.":1217.68885,"DeltaM..ppm.":-0.36,"Deltam.z..Da.":-0.00015,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":577063.6875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":21.16208,"Ion.Inject.Time..ms.":22,"RT..min.":13.1564,"First.Scan":12377,"Last.Scan":12377,"Master.Scan.s.":12366,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_01.raw","File.ID":"F19","Quan.Info":"","Peptides.Matched":274,"XCorr":2.32,"X..Protein.Groups":1,"Percolator.q.Value":0.00036,"Percolator.PEP":0.00001,"Percolator.SVMScore":1.866,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (R4)","Search.ID":"R","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MGSINNNFNTNNNSNTDLDRDWK","Annotated.Sequence":"[-].mGSINNNFNTNNNsNTDLDRDWK.[T]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S14(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P39517","Master.Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","Protein.Accessions":"P39517","Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.0714,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1338.05911,"Contaminant":"False","Aligned.m.z..Da.":1338.05952,"MH...Da.":2675.11094,"Theo..MH...Da.":2675.11179,"DeltaM..ppm.":-0.32,"Deltam.z..Da.":-0.00043,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":492657.625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":13.5775,"First.Scan":12920,"Last.Scan":12920,"Master.Scan.s.":12912,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_01.raw","File.ID":"F19","Quan.Info":"","Peptides.Matched":2149,"XCorr":5.32,"X..Protein.Groups":1,"Percolator.q.Value":0.00036,"Percolator.PEP":6.932e-09,"Percolator.SVMScore":3.262,"ptmRS..Binomial.Peptide.Score":517.92,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(21): 2244.95; y1+-H(3) O(4) P(15): 1788.79; y1+-H(3) O(4) P(14): 1674.75; y1+-H(3) O(4) P(13): 1573.70; y1+-H(3) O(4) P(12): 1459.66; y1+-H(3) O(4) P(10): 1231.57; b2+-H(3) O(4) P(19): 987.41; y2+-H(3) O(4) P(19): 1139.50; y1+(9): 1162.55; y1+(8): 1048.51","ptmRS..Best.Site.Probabilities":"S14(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(3): 0.0; T(10): 0.0; S(14): 100.0; T(16): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (R4)","Search.ID":"R","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"AIMPIIEGHDVLAQAQSGTGK","Annotated.Sequence":"[R].AImPIIEGHDVLAQAQSGTGK.[T]","Modifications":"M3(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.7076,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":718.04031,"Contaminant":"False","Aligned.m.z..Da.":718.04042,"MH...Da.":2152.10637,"Theo..MH...Da.":2152.10668,"DeltaM..ppm.":-0.14,"Deltam.z..Da.":-0.0001,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":649701.6875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":11.17852,"Ion.Inject.Time..ms.":22,"RT..min.":12.7394,"First.Scan":11821,"Last.Scan":11821,"Master.Scan.s.":11820,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_01.raw","File.ID":"F19","Quan.Info":"","Peptides.Matched":998,"XCorr":4.48,"X..Protein.Groups":1,"Percolator.q.Value":0.00036,"Percolator.PEP":5.414e-10,"Percolator.SVMScore":3.743,"Precursor.Area":811112.875,"Apex.RT..min.":12.76,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (K4)","Search.ID":"K","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEySDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); Y12(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.3552,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40591,"Contaminant":"False","Aligned.m.z..Da.":967.40566,"MH...Da.":2900.20318,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":1.33,"Deltam.z..Da.":0.00129,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":155478.21875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":16.8288,"First.Scan":17141,"Last.Scan":17141,"Master.Scan.s.":17135,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_02.raw","File.ID":"F26","Quan.Info":"","Peptides.Matched":2562,"XCorr":2.59,"X..Protein.Groups":1,"Percolator.q.Value":0.00036,"Percolator.PEP":3.642e-06,"Percolator.SVMScore":2.176,"ptmRS..Binomial.Peptide.Score":282.72,"ptmRS..Isoform.Confidence.Probability":0.5,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"Y12(Phospho): 50; S13(Phospho): 50","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 50.0; S(13): 50.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (K4)","Search.ID":"K","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].msEGITDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S2(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":3,"DeltaScore":0.1375,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1096.4674,"Contaminant":"False","Aligned.m.z..Da.":1096.46725,"MH...Da.":2191.92751,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":-0.15,"Deltam.z..Da.":-0.00016,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":432070,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":78.63202,"Ion.Inject.Time..ms.":22,"RT..min.":15.9631,"First.Scan":16050,"Last.Scan":16050,"Master.Scan.s.":16046,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_02.raw","File.ID":"F26","Quan.Info":"","Peptides.Matched":1955,"XCorr":2.91,"X..Protein.Groups":1,"Percolator.q.Value":0.00036,"Percolator.PEP":0.00001,"Percolator.SVMScore":1.903,"Precursor.Area":21676970,"Apex.RT..min.":15.78,"ptmRS..Binomial.Peptide.Score":289.36,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(2): 112.04; b1+-H(3) O(4) P(3): 241.08; b1+-H(3) O(4) P(4): 298.10; b1+-H(3) O(4) P(5): 411.19; b1+-H(3) O(4) P(6): 512.24; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+-H(3) O(4) P(9): 869.39","ptmRS..Best.Site.Probabilities":"S2(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 100.0; T(6): 0.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (K4)","Search.ID":"K","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSSPLHRLSPTGK","Annotated.Sequence":"[R].mSsPLHRLsPTGK.[S]","Modifications":"M1(Oxidation); S3(Phospho); S9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.4894,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":529.56461,"Contaminant":"False","Aligned.m.z..Da.":529.56469,"MH...Da.":1586.67927,"Theo..MH...Da.":1586.67977,"DeltaM..ppm.":-0.31,"Deltam.z..Da.":-0.00017,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3419345.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":12.97035,"Ion.Inject.Time..ms.":22,"RT..min.":7.7177,"First.Scan":5548,"Last.Scan":5548,"Master.Scan.s.":5542,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_02.raw","File.ID":"F26","Quan.Info":"","Peptides.Matched":1009,"XCorr":3.78,"X..Protein.Groups":1,"Percolator.q.Value":0.00036,"Percolator.PEP":0.00001,"Percolator.SVMScore":1.914,"Precursor.Area":6711585.5,"Apex.RT..min.":7.74,"ptmRS..Binomial.Peptide.Score":263.17,"ptmRS..Isoform.Confidence.Probability":0.993,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(8): 877.50; y1+-H(3) O(4) P(7): 740.44; y2+-H(3) O(4) P(12): 671.34; y2+-H(3) O(4) P(10): 544.32; y2+-H(3) O(4) P(8): 439.25; y1+(4): 402.23","ptmRS..Best.Site.Probabilities":"S3(Phospho): 100; S9(Phospho): 99.3","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(3): 100.0; S(9): 99.3; T(11): 0.7"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (K4)","Search.ID":"K","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LQETNPEEVPK","Annotated.Sequence":"[K].LQETNPEEVPK.[F]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5069,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":642.32788,"Contaminant":"False","Aligned.m.z..Da.":642.32797,"MH...Da.":1283.64849,"Theo..MH...Da.":1283.64777,"DeltaM..ppm.":0.56,"Deltam.z..Da.":0.00036,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2688545.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":10.7998,"Ion.Inject.Time..ms.":22,"RT..min.":7.6406,"First.Scan":5436,"Last.Scan":5436,"Master.Scan.s.":5425,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_02.raw","File.ID":"F26","Quan.Info":"","Peptides.Matched":580,"XCorr":2.88,"X..Protein.Groups":1,"Percolator.q.Value":0.00036,"Percolator.PEP":8.302e-06,"Percolator.SVMScore":2.013,"Precursor.Area":9268322,"Apex.RT..min.":7.66,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (K4)","Search.ID":"K","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"IGNNELLHR","Annotated.Sequence":"[K].IGNNELLHR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q03558","Master.Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","Protein.Accessions":"Q03558","Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.5765,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":355.86477,"Contaminant":"False","Aligned.m.z..Da.":355.86482,"MH...Da.":1065.57976,"Theo..MH...Da.":1065.57997,"DeltaM..ppm.":-0.2,"Deltam.z..Da.":-0.00007,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2166069,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":11.47081,"Ion.Inject.Time..ms.":22,"RT..min.":7.585,"First.Scan":5352,"Last.Scan":5352,"Master.Scan.s.":5347,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_02.raw","File.ID":"F26","Quan.Info":"","Peptides.Matched":300,"XCorr":2.55,"X..Protein.Groups":1,"Percolator.q.Value":0.00036,"Percolator.PEP":0.00001,"Percolator.SVMScore":1.973,"Precursor.Area":1517502.5,"Apex.RT..min.":7.59,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KDELTLEGIK","Annotated.Sequence":"[K].KDELTLEGIK.[Q]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.5749,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":382.55142,"Contaminant":"False","Aligned.m.z..Da.":382.55146,"MH...Da.":1145.63971,"Theo..MH...Da.":1145.64123,"DeltaM..ppm.":-1.32,"Deltam.z..Da.":-0.00051,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":5235053.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":16.6,"RT..min.":10.2961,"First.Scan":8930,"Last.Scan":8930,"Master.Scan.s.":8925,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":367,"XCorr":3.27,"X..Protein.Groups":1,"Percolator.q.Value":0.00039,"Percolator.PEP":0.00195,"Percolator.SVMScore":0.954,"Precursor.Area":5329939.5,"Apex.RT..min.":10.3,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DLEQENVEK","Annotated.Sequence":"[K].DLEQENVEK.[E]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5422,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":552.26445,"Contaminant":"False","Aligned.m.z..Da.":552.26446,"MH...Da.":1103.52162,"Theo..MH...Da.":1103.52151,"DeltaM..ppm.":0.1,"Deltam.z..Da.":0.00006,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":7782262.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":3.99901,"Ion.Inject.Time..ms.":16.45,"RT..min.":6.462,"First.Scan":3848,"Last.Scan":3848,"Master.Scan.s.":3842,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":572,"XCorr":2.25,"X..Protein.Groups":1,"Percolator.q.Value":0.00039,"Percolator.PEP":0.00218,"Percolator.SVMScore":0.924,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"IGNNELLHR","Annotated.Sequence":"[K].IGNNELLHR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q03558","Master.Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","Protein.Accessions":"Q03558","Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.4251,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":355.86446,"Contaminant":"False","Aligned.m.z..Da.":355.86447,"MH...Da.":1065.57883,"Theo..MH...Da.":1065.57997,"DeltaM..ppm.":-1.07,"Deltam.z..Da.":-0.00038,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2792861.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":9.69763,"Ion.Inject.Time..ms.":22,"RT..min.":7.5986,"First.Scan":5197,"Last.Scan":5197,"Master.Scan.s.":5194,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":292,"XCorr":2.07,"X..Protein.Groups":1,"Percolator.q.Value":0.00039,"Percolator.PEP":0.00152,"Percolator.SVMScore":1.02,"Precursor.Area":4479578,"Apex.RT..min.":7.62,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].msHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss); S2(Phospho); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.5444,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":980.0569,"Contaminant":"False","Aligned.m.z..Da.":980.05706,"MH...Da.":2938.15615,"Theo..MH...Da.":2938.15509,"DeltaM..ppm.":0.36,"Deltam.z..Da.":0.00035,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":407775.34375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":6.49357,"Ion.Inject.Time..ms.":22,"RT..min.":15.0689,"First.Scan":15033,"Last.Scan":15033,"Master.Scan.s.":15022,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":1411,"XCorr":2.48,"X..Protein.Groups":1,"Percolator.q.Value":0.00039,"Percolator.PEP":0.00184,"Percolator.SVMScore":0.969,"ptmRS..Binomial.Peptide.Score":160.3,"ptmRS..Isoform.Confidence.Probability":0.5,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"S2(Phospho): 99.98; Y12(Phospho): 50.01; S13(Phospho): 50.01","ptmRS..Phospho.Site.Probabilities":"S(2): 100.0; Y(12): 50.0; S(13): 50.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FFISNPDLVDR","Annotated.Sequence":"[R].FFIsNPDLVDR.[L]","Modifications":"S4(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q03558","Master.Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","Protein.Accessions":"Q03558","Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7357,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":701.82136,"Contaminant":"False","Aligned.m.z..Da.":701.82133,"MH...Da.":1402.63544,"Theo..MH...Da.":1402.64026,"DeltaM..ppm.":-3.43,"Deltam.z..Da.":-0.00241,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":544449.0625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":56.61333,"Ion.Inject.Time..ms.":22,"RT..min.":16.9663,"First.Scan":17465,"Last.Scan":17465,"Master.Scan.s.":17453,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":802,"XCorr":2.27,"X..Protein.Groups":1,"Percolator.q.Value":0.00039,"Percolator.PEP":0.00183,"Percolator.SVMScore":0.97,"ptmRS..Binomial.Peptide.Score":306.73,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"S4(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(4): 100.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Z4)","Search.ID":"Z","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ILISTDLLAR","Annotated.Sequence":"[R].ILISTDLLAR.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.4961,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":557.84568,"Contaminant":"False","Aligned.m.z..Da.":557.8457,"MH...Da.":1114.68408,"Theo..MH...Da.":1114.68304,"DeltaM..ppm.":0.94,"Deltam.z..Da.":0.00052,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":324234.96875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":21.2778,"Ion.Inject.Time..ms.":22,"RT..min.":15.6296,"First.Scan":15503,"Last.Scan":15503,"Master.Scan.s.":15502,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_05.raw","File.ID":"F11","Quan.Info":"","Peptides.Matched":124,"XCorr":1.29,"X..Protein.Groups":1,"Percolator.q.Value":0.00039,"Percolator.PEP":0.00263,"Percolator.SVMScore":0.752,"Precursor.Area":606895,"Apex.RT..min.":15.6,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (H4)","Search.ID":"H","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LEAGLSDSK","Annotated.Sequence":"[K].LEAGLsDSK.[Q]","Modifications":"S6(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.0591,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":500.22324,"Contaminant":"False","Aligned.m.z..Da.":500.22334,"MH...Da.":999.43921,"Theo..MH...Da.":999.43943,"DeltaM..ppm.":-0.22,"Deltam.z..Da.":-0.00011,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2606360.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":27.33052,"Ion.Inject.Time..ms.":22,"RT..min.":7.675,"First.Scan":5910,"Last.Scan":5910,"Master.Scan.s.":5900,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_05.raw","File.ID":"F29","Quan.Info":"","Peptides.Matched":608,"XCorr":2.37,"X..Protein.Groups":1,"Percolator.q.Value":0.00039,"Percolator.PEP":0.00129,"Percolator.SVMScore":0.985,"Precursor.Area":3070416.5,"Apex.RT..min.":7.69,"ptmRS..Binomial.Peptide.Score":255.73,"ptmRS..Isoform.Confidence.Probability":0.996,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(8): 788.38; y1+-H(3) O(4) P(7): 659.34; y1+-H(3) O(4) P(6): 588.30; y1+-H(3) O(4) P(5): 531.28; y1+-H(3) O(4) P(4): 418.19; y2+-H(3) O(4) P(6): 294.65; y1+(3): 349.17; y1+(2): 234.14","ptmRS..Best.Site.Probabilities":"S6(Phospho): 99.6","ptmRS..Phospho.Site.Probabilities":"S(6): 99.6; S(8): 0.4"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (H4)","Search.ID":"H","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LEAGLSDSK","Annotated.Sequence":"[K].LEAGLSDSK.[Q]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1852,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":460.23998,"Contaminant":"False","Aligned.m.z..Da.":460.24,"MH...Da.":919.47269,"Theo..MH...Da.":919.4731,"DeltaM..ppm.":-0.44,"Deltam.z..Da.":-0.0002,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2557108.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":42.67237,"Ion.Inject.Time..ms.":22,"RT..min.":6.6445,"First.Scan":4555,"Last.Scan":4555,"Master.Scan.s.":4548,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_05.raw","File.ID":"F29","Quan.Info":"","Peptides.Matched":407,"XCorr":1.62,"X..Protein.Groups":1,"Percolator.q.Value":0.00039,"Percolator.PEP":0.00123,"Percolator.SVMScore":0.997,"Precursor.Area":1478227.75,"Apex.RT..min.":6.64,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (T4)","Search.ID":"T","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LQQTAFDKK","Annotated.Sequence":"[R].LQQTAFDKK.[S]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.3803,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":360.20146,"Contaminant":"False","Aligned.m.z..Da.":360.2015,"MH...Da.":1078.58982,"Theo..MH...Da.":1078.58914,"DeltaM..ppm.":0.64,"Deltam.z..Da.":0.00023,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2094213.625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":25.50028,"Ion.Inject.Time..ms.":22,"RT..min.":5.5112,"First.Scan":2734,"Last.Scan":2734,"Master.Scan.s.":2731,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_05.raw","File.ID":"F17","Quan.Info":"","Peptides.Matched":392,"XCorr":2.34,"X..Protein.Groups":1,"Percolator.q.Value":0.00039,"Percolator.PEP":0.00293,"Percolator.SVMScore":0.789,"Precursor.Area":1489800,"Apex.RT..min.":5.51,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (T4)","Search.ID":"T","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ELALQIQK","Annotated.Sequence":"[R].ELALQIQK.[V]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.2455,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":471.78457,"Contaminant":"False","Aligned.m.z..Da.":471.7846,"MH...Da.":942.56187,"Theo..MH...Da.":942.56186,"DeltaM..ppm.":0.01,"Deltam.z..Da.":1e-05,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":557032.6875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":7.77677,"Ion.Inject.Time..ms.":22,"RT..min.":10.6458,"First.Scan":9866,"Last.Scan":9866,"Master.Scan.s.":9857,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_05.raw","File.ID":"F17","Quan.Info":"","Peptides.Matched":292,"XCorr":2.2,"X..Protein.Groups":1,"Percolator.q.Value":0.00039,"Percolator.PEP":0.00344,"Percolator.SVMScore":0.757,"Precursor.Area":273990.8125,"Apex.RT..min.":10.65,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (T4)","Search.ID":"T","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"TAVFYGGTPISK","Annotated.Sequence":"[K].TAVFYGGTPISK.[D]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.498,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":620.83081,"Contaminant":"False","Aligned.m.z..Da.":620.83085,"MH...Da.":1240.65435,"Theo..MH...Da.":1240.65721,"DeltaM..ppm.":-2.31,"Deltam.z..Da.":-0.00143,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":159090.84375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":84.03062,"Ion.Inject.Time..ms.":22,"RT..min.":11.1891,"First.Scan":10519,"Last.Scan":10519,"Master.Scan.s.":10509,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_05.raw","File.ID":"F17","Quan.Info":"","Peptides.Matched":377,"XCorr":2.45,"X..Protein.Groups":1,"Percolator.q.Value":0.00039,"Percolator.PEP":0.00126,"Percolator.SVMScore":0.957,"Precursor.Area":181909.57812,"Apex.RT..min.":11.2,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (O4)","Search.ID":"O","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"AAEAGETGAATSATEGDNNNNTAAGDKK","Annotated.Sequence":"[K].AAEAGETGAATSATEGDNNNNTAAGDKK.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.7503,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":879.39447,"Contaminant":"False","Aligned.m.z..Da.":879.39433,"MH...Da.":2636.16885,"Theo..MH...Da.":2636.16665,"DeltaM..ppm.":0.83,"Deltam.z..Da.":0.00073,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2190042.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":4.9286,"First.Scan":1693,"Last.Scan":1693,"Master.Scan.s.":1681,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_04.raw","File.ID":"F22","Quan.Info":"","Peptides.Matched":3434,"XCorr":7.57,"X..Protein.Groups":1,"Percolator.q.Value":0.0004,"Percolator.PEP":6.4388e-18,"Percolator.SVMScore":10.1218,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (O4)","Search.ID":"O","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LQETNPEEVPKFEK","Annotated.Sequence":"[K].LQETNPEEVPKFEK.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.6075,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":563.2897,"Contaminant":"False","Aligned.m.z..Da.":563.28972,"MH...Da.":1687.85454,"Theo..MH...Da.":1687.85374,"DeltaM..ppm.":0.47,"Deltam.z..Da.":0.00027,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2005615.125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":43.70299,"Ion.Inject.Time..ms.":22,"RT..min.":9.3233,"First.Scan":7508,"Last.Scan":7508,"Master.Scan.s.":7505,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_04.raw","File.ID":"F22","Quan.Info":"","Peptides.Matched":879,"XCorr":1.86,"X..Protein.Groups":1,"Percolator.q.Value":0.0004,"Percolator.PEP":7.82e-07,"Percolator.SVMScore":2.356,"Precursor.Area":1408072.5,"Apex.RT..min.":9.32,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (O4)","Search.ID":"O","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"AAEAGETGAATSATEGDNNNNTAAGDKK","Annotated.Sequence":"[K].AAEAGETGAATsATEGDNNNNTAAGDKK.[G]","Modifications":"S12(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0426,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":906.04983,"Contaminant":"False","Aligned.m.z..Da.":906.04971,"MH...Da.":2716.13494,"Theo..MH...Da.":2716.13298,"DeltaM..ppm.":0.72,"Deltam.z..Da.":0.00065,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1242335.125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":7.25583,"Ion.Inject.Time..ms.":22,"RT..min.":5.0207,"First.Scan":1783,"Last.Scan":1783,"Master.Scan.s.":1772,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_04.raw","File.ID":"F22","Quan.Info":"","Peptides.Matched":2514,"XCorr":6.81,"X..Protein.Groups":1,"Percolator.q.Value":0.0004,"Percolator.PEP":3.152e-15,"Percolator.SVMScore":6.077,"ptmRS..Binomial.Peptide.Score":516.13,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(15): 1300.57; y1+-H(3) O(4) P(18): 1789.79; y1+-H(3) O(4) P(17): 1688.75; y2+-H(3) O(4) P(25): 1174.02; y2+-H(3) O(4) P(21): 994.95; y2+-H(3) O(4) P(19): 930.92; y2+-H(3) O(4) P(18): 895.40; y2+-H(3) O(4) P(17): 844.88","ptmRS..Best.Site.Probabilities":"S12(Phospho): 99.99","ptmRS..Phospho.Site.Probabilities":"T(7): 0.0; T(11): 0.0; S(12): 100.0; T(14): 0.0; T(22): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (O4)","Search.ID":"O","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"HSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[R].HSDsYsENETNHTNVPISSTGGTNNK.[T]","Modifications":"S4(Phospho); S6(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.1735,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":984.05608,"Contaminant":"False","Aligned.m.z..Da.":984.0562,"MH...Da.":2950.1537,"Theo..MH...Da.":2950.15241,"DeltaM..ppm.":0.44,"Deltam.z..Da.":0.00043,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":4179689.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":8.0418,"First.Scan":5961,"Last.Scan":5961,"Master.Scan.s.":5958,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_04.raw","File.ID":"F22","Quan.Info":"","Peptides.Matched":1168,"XCorr":6.8,"X..Protein.Groups":1,"Percolator.q.Value":0.0004,"Percolator.PEP":5.749e-11,"Percolator.SVMScore":4.148,"Precursor.Area":4295366,"Apex.RT..min.":8.05,"ptmRS..Binomial.Peptide.Score":669.8,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(6): 739.21; y2+-H(3) O(4) P(23): 1257.03; y2+-H(3) O(4) P(22): 1173.53; y2+-H(3) O(4) P(21): 1092.00; b1+- -2H(3) O(4) P -2H(3) O(4) P(6): 641.23; b1+- -2H(3) O(4) P -2H(3) O(4) P(11): 1228.45; b1+- -2H(3) O(4) P -2H(3) O(4) P(15): 1679.67; b2+- -2H(3) O(4) P -2H(3) O(4) P(13): 733.78; b2+- -2H(3) O(4) P -2H(3) O(4) P(14): 790.80; b2+- -2H(3) O(4) P -2H(3) O(4) P(15): 840.34","ptmRS..Best.Site.Probabilities":"S4(Phospho): 100; S6(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(4): 100.0; Y(5): 0.0; S(6): 100.0; T(10): 0.0; T(13): 0.0; S(18): 0.0; S(19): 0.0; T(20): 0.0; T(23): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (O4)","Search.ID":"O","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"MGSINNNFNTNNNSNTDLDRDWK","Annotated.Sequence":"[-].mGSINNNFNTNNNsNTDLDRDWK.[T]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S14(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P39517","Master.Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","Protein.Accessions":"P39517","Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.0297,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1338.05685,"Contaminant":"False","Aligned.m.z..Da.":1338.05737,"MH...Da.":2675.10643,"Theo..MH...Da.":2675.11179,"DeltaM..ppm.":-2,"Deltam.z..Da.":-0.00268,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":441574.3125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":13.5859,"First.Scan":13045,"Last.Scan":13045,"Master.Scan.s.":13043,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_04.raw","File.ID":"F22","Quan.Info":"","Peptides.Matched":2140,"XCorr":4.04,"X..Protein.Groups":1,"Percolator.q.Value":0.0004,"Percolator.PEP":8.187e-09,"Percolator.SVMScore":3.214,"ptmRS..Binomial.Peptide.Score":574.89,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(14): 1674.75; y1+-H(3) O(4) P(13): 1573.70; y1+-H(3) O(4) P(12): 1459.66; y1+-H(3) O(4) P(10): 1231.57; b2+-H(3) O(4) P(19): 987.41; b2+-H(3) O(4) P(20): 1065.47; y2+-H(3) O(4) P(19): 1139.50; y2+-H(3) O(4) P(16): 968.43; y1+(9): 1162.55; y1+(8): 1048.51","ptmRS..Best.Site.Probabilities":"S14(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(3): 0.0; T(10): 0.0; S(14): 100.0; T(16): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (O4)","Search.ID":"O","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"TGTFSIAALQR","Annotated.Sequence":"[K].TGTFSIAALQR.[I]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7701,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":582.82202,"Contaminant":"False","Aligned.m.z..Da.":582.82223,"MH...Da.":1164.63676,"Theo..MH...Da.":1164.63715,"DeltaM..ppm.":-0.33,"Deltam.z..Da.":-0.00019,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1029657.1875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":3.86927,"Ion.Inject.Time..ms.":22,"RT..min.":13.3562,"First.Scan":12747,"Last.Scan":12747,"Master.Scan.s.":12744,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_04.raw","File.ID":"F22","Quan.Info":"","Peptides.Matched":364,"XCorr":3.35,"X..Protein.Groups":1,"Percolator.q.Value":0.0004,"Percolator.PEP":5.154e-07,"Percolator.SVMScore":2.434,"Precursor.Area":1817332.625,"Apex.RT..min.":13.37,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (O4)","Search.ID":"O","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].msEGITDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S2(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1415,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1096.46692,"Contaminant":"False","Aligned.m.z..Da.":1096.46679,"MH...Da.":2191.92657,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":-0.58,"Deltam.z..Da.":-0.00064,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":306472.4375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":16.0414,"First.Scan":16158,"Last.Scan":16158,"Master.Scan.s.":16156,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_04.raw","File.ID":"F22","Quan.Info":"","Peptides.Matched":1954,"XCorr":3.11,"X..Protein.Groups":1,"Percolator.q.Value":0.0004,"Percolator.PEP":2.961e-08,"Percolator.SVMScore":2.972,"Precursor.Area":17286162,"Apex.RT..min.":15.79,"ptmRS..Binomial.Peptide.Score":458.93,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(2): 112.04; b1+-H(3) O(4) P(3): 241.08; b1+-H(3) O(4) P(4): 298.10; b1+-H(3) O(4) P(5): 411.19; b1+-H(3) O(4) P(6): 512.24; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+-H(3) O(4) P(9): 869.39; b1+(4): 396.08; y1+(14): 1683.77","ptmRS..Best.Site.Probabilities":"S2(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 100.0; T(6): 0.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (O4)","Search.ID":"O","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEySDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); Y12(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.3889,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40846,"Contaminant":"False","Aligned.m.z..Da.":967.40834,"MH...Da.":2900.21083,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":3.97,"Deltam.z..Da.":0.00383,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":287624.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":22.02582,"Ion.Inject.Time..ms.":22,"RT..min.":16.0734,"First.Scan":16198,"Last.Scan":16198,"Master.Scan.s.":16195,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_04.raw","File.ID":"F22","Quan.Info":"","Peptides.Matched":2587,"XCorr":4.14,"X..Protein.Groups":1,"Percolator.q.Value":0.0004,"Percolator.PEP":1.449e-06,"Percolator.SVMScore":2.24,"ptmRS..Binomial.Peptide.Score":327.52,"ptmRS..Isoform.Confidence.Probability":0.006,"ptmRS..Isoform.Group.Report":"b1+(12): 1344.56","ptmRS..Best.Site.Probabilities":"S13(Phospho): 99.44","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.6; S(13): 99.4; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (O4)","Search.ID":"O","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].msEGITDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S2(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":3,"DeltaScore":0.1504,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1096.46728,"Contaminant":"False","Aligned.m.z..Da.":1096.46716,"MH...Da.":2191.92729,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":-0.25,"Deltam.z..Da.":-0.00028,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2208658.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":49.42809,"Ion.Inject.Time..ms.":22,"RT..min.":15.8511,"First.Scan":15921,"Last.Scan":15921,"Master.Scan.s.":15909,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_04.raw","File.ID":"F22","Quan.Info":"","Peptides.Matched":1943,"XCorr":2.66,"X..Protein.Groups":1,"Percolator.q.Value":0.0004,"Percolator.PEP":0.00002,"Percolator.SVMScore":1.728,"Precursor.Area":17286162,"Apex.RT..min.":15.79,"ptmRS..Binomial.Peptide.Score":269.5,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(2): 112.04; b1+-H(3) O(4) P(3): 241.08; b1+-H(3) O(4) P(4): 298.10; b1+-H(3) O(4) P(5): 411.19; b1+-H(3) O(4) P(6): 512.24; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+-H(3) O(4) P(9): 869.39","ptmRS..Best.Site.Probabilities":"S2(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 100.0; T(6): 0.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (O4)","Search.ID":"O","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DIFSNDELLSDAYDAK","Annotated.Sequence":"[K].DIFSNDELLSDAYDAK.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6994,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":908.41798,"Contaminant":"False","Aligned.m.z..Da.":908.41785,"MH...Da.":1815.82869,"Theo..MH...Da.":1815.82831,"DeltaM..ppm.":0.21,"Deltam.z..Da.":0.00019,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":478812.46875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":17.65961,"Ion.Inject.Time..ms.":22,"RT..min.":17.0591,"First.Scan":17433,"Last.Scan":17433,"Master.Scan.s.":17429,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_04.raw","File.ID":"F22","Quan.Info":"","Peptides.Matched":1551,"XCorr":3.56,"X..Protein.Groups":1,"Percolator.q.Value":0.0004,"Percolator.PEP":8.02e-09,"Percolator.SVMScore":3.218,"Precursor.Area":2756189,"Apex.RT..min.":17.08,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (O4)","Search.ID":"O","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0515,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40293,"Contaminant":"False","Aligned.m.z..Da.":967.40284,"MH...Da.":2900.19423,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":-1.76,"Deltam.z..Da.":-0.0017,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":477460.1875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":11.04352,"Ion.Inject.Time..ms.":22,"RT..min.":15.5546,"First.Scan":15546,"Last.Scan":15546,"Master.Scan.s.":15545,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_04.raw","File.ID":"F22","Quan.Info":"","Peptides.Matched":2267,"XCorr":4.85,"X..Protein.Groups":1,"Percolator.q.Value":0.0004,"Percolator.PEP":7.737e-09,"Percolator.SVMScore":3.225,"Precursor.Area":3672541.25,"Apex.RT..min.":15.36,"ptmRS..Binomial.Peptide.Score":434.18,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(13): 1413.58; b1+-H(3) O(4) P(14): 1528.61; y1+-H(3) O(4) P(14): 1621.73; y2+-H(3) O(4) P(14): 811.37; b1+(12): 1344.56","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (O4)","Search.ID":"O","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MFILDEADEMLSSGFK","Annotated.Sequence":"[K].mFILDEADEmLSSGFK.[E]","Modifications":"M1(Oxidation); M10(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.705,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":932.91893,"Contaminant":"False","Aligned.m.z..Da.":932.91879,"MH...Da.":1864.8306,"Theo..MH...Da.":1864.83433,"DeltaM..ppm.":-2,"Deltam.z..Da.":-0.00187,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":515360.6875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":16.6936,"First.Scan":16971,"Last.Scan":16971,"Master.Scan.s.":16961,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_04.raw","File.ID":"F22","Quan.Info":"","Peptides.Matched":1848,"XCorr":2.78,"X..Protein.Groups":1,"Percolator.q.Value":0.0004,"Percolator.PEP":1.438e-09,"Percolator.SVMScore":3.541,"Precursor.Area":448376.375,"Apex.RT..min.":16.7,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (O4)","Search.ID":"O","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].mSEGItDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); T6(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.1146,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":731.31489,"Contaminant":"False","Aligned.m.z..Da.":731.31483,"MH...Da.":2191.93012,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":1.04,"Deltam.z..Da.":0.00076,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":391043.96875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":68.24627,"Ion.Inject.Time..ms.":22,"RT..min.":15.5364,"First.Scan":15524,"Last.Scan":15524,"Master.Scan.s.":15519,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_04.raw","File.ID":"F22","Quan.Info":"","Peptides.Matched":2059,"XCorr":4.1,"X..Protein.Groups":1,"Percolator.q.Value":0.0004,"Percolator.PEP":3.046e-08,"Percolator.SVMScore":2.967,"Precursor.Area":369570.875,"Apex.RT..min.":15.55,"ptmRS..Binomial.Peptide.Score":392.91,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+-H(3) O(4) P(9): 869.39; b1+-H(3) O(4) P(10): 998.43; b1+(2): 130.05; b1+(3): 259.09; b1+(4): 316.11; b1+(5): 429.20; b2+(3): 130.05","ptmRS..Best.Site.Probabilities":"T6(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; T(6): 100.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (O4)","Search.ID":"O","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].mSEGItDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); T6(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.109,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1096.46956,"Contaminant":"False","Aligned.m.z..Da.":1096.46946,"MH...Da.":2191.93184,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":1.82,"Deltam.z..Da.":0.002,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":300649.0625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":15.5285,"First.Scan":15515,"Last.Scan":15515,"Master.Scan.s.":15506,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_04.raw","File.ID":"F22","Quan.Info":"","Peptides.Matched":2017,"XCorr":4.68,"X..Protein.Groups":1,"Percolator.q.Value":0.0004,"Percolator.PEP":1.08e-10,"Percolator.SVMScore":4.029,"Precursor.Area":408460.40625,"Apex.RT..min.":15.55,"ptmRS..Binomial.Peptide.Score":478.66,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(6): 512.24; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+-H(3) O(4) P(9): 869.39; b1+-H(3) O(4) P(10): 998.43; y1+-H(3) O(4) P(14): 1665.76; b2+-H(3) O(4) P(16): 835.38; b1+(3): 259.09; b1+(4): 316.11; b1+(5): 429.20","ptmRS..Best.Site.Probabilities":"T6(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; T(6): 100.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (O4)","Search.ID":"O","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEySDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); Y12(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.4534,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40521,"Contaminant":"False","Aligned.m.z..Da.":967.40508,"MH...Da.":2900.2011,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":0.61,"Deltam.z..Da.":0.00059,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":237690.95312,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":66.37341,"Ion.Inject.Time..ms.":22,"RT..min.":17.1148,"First.Scan":17505,"Last.Scan":17505,"Master.Scan.s.":17494,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_04.raw","File.ID":"F22","Quan.Info":"","Peptides.Matched":2508,"XCorr":3.22,"X..Protein.Groups":1,"Percolator.q.Value":0.0004,"Percolator.PEP":1.146e-06,"Percolator.SVMScore":2.284,"ptmRS..Binomial.Peptide.Score":278.46,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"b2+-H(3) O(4) P(18): 1014.90; y2+-H(3) O(4) P(14): 811.37","ptmRS..Best.Site.Probabilities":"S13(Phospho): 99.99","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (O4)","Search.ID":"O","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FDDMELDENLLR","Annotated.Sequence":"[K].FDDmELDENLLR.[G]","Modifications":"M4(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5948,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":763.34456,"Contaminant":"False","Aligned.m.z..Da.":763.34471,"MH...Da.":1525.68185,"Theo..MH...Da.":1525.6839,"DeltaM..ppm.":-1.34,"Deltam.z..Da.":-0.00103,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":989502.625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":72.73725,"Ion.Inject.Time..ms.":22,"RT..min.":14.4786,"First.Scan":14191,"Last.Scan":14191,"Master.Scan.s.":14187,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_04.raw","File.ID":"F22","Quan.Info":"","Peptides.Matched":965,"XCorr":2.69,"X..Protein.Groups":1,"Percolator.q.Value":0.0004,"Percolator.PEP":1.193e-06,"Percolator.SVMScore":2.276,"Precursor.Area":918278.1875,"Apex.RT..min.":14.49,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (O4)","Search.ID":"O","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FFISNPDLVDR","Annotated.Sequence":"[R].FFISNPDLVDR.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q03558","Master.Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","Protein.Accessions":"Q03558","Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.748,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":661.83944,"Contaminant":"False","Aligned.m.z..Da.":661.83953,"MH...Da.":1322.67162,"Theo..MH...Da.":1322.67393,"DeltaM..ppm.":-1.75,"Deltam.z..Da.":-0.00115,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":767266.5625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":28.43949,"Ion.Inject.Time..ms.":22,"RT..min.":14.662,"First.Scan":14421,"Last.Scan":14421,"Master.Scan.s.":14414,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_04.raw","File.ID":"F22","Quan.Info":"","Peptides.Matched":665,"XCorr":2.46,"X..Protein.Groups":1,"Percolator.q.Value":0.0004,"Percolator.PEP":2.705e-06,"Percolator.SVMScore":2.122,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (O4)","Search.ID":"O","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DIFSNDELLSDAYDAK","Annotated.Sequence":"[K].DIFSNDELLSDAYDAK.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.694,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":605.94797,"Contaminant":"False","Aligned.m.z..Da.":605.94788,"MH...Da.":1815.82935,"Theo..MH...Da.":1815.82831,"DeltaM..ppm.":0.57,"Deltam.z..Da.":0.00035,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":558901.1875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":8.34089,"Ion.Inject.Time..ms.":22,"RT..min.":17.0693,"First.Scan":17446,"Last.Scan":17446,"Master.Scan.s.":17442,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_04.raw","File.ID":"F22","Quan.Info":"","Peptides.Matched":1558,"XCorr":2.81,"X..Protein.Groups":1,"Percolator.q.Value":0.0004,"Percolator.PEP":6.646e-06,"Percolator.SVMScore":1.953,"Precursor.Area":671072.375,"Apex.RT..min.":17.08,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (O4)","Search.ID":"O","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FTVSAIYSDLPQQER","Annotated.Sequence":"[K].FTVSAIYSDLPQQER.[D]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6838,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":877.44015,"Contaminant":"False","Aligned.m.z..Da.":877.44016,"MH...Da.":1753.87302,"Theo..MH...Da.":1753.87554,"DeltaM..ppm.":-1.44,"Deltam.z..Da.":-0.00126,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":575886.0625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":15.0307,"First.Scan":14888,"Last.Scan":14888,"Master.Scan.s.":14882,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_04.raw","File.ID":"F22","Quan.Info":"","Peptides.Matched":1146,"XCorr":4.08,"X..Protein.Groups":1,"Percolator.q.Value":0.0004,"Percolator.PEP":1.522e-10,"Percolator.SVMScore":3.964,"Precursor.Area":730676.5,"Apex.RT..min.":15.04,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (O4)","Search.ID":"O","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEySDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); Y12(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.4426,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40338,"Contaminant":"False","Aligned.m.z..Da.":967.4034,"MH...Da.":2900.19561,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":-1.28,"Deltam.z..Da.":-0.00124,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":305044.8125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":15.0422,"First.Scan":14903,"Last.Scan":14903,"Master.Scan.s.":14895,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_04.raw","File.ID":"F22","Quan.Info":"","Peptides.Matched":2273,"XCorr":4.27,"X..Protein.Groups":1,"Percolator.q.Value":0.0004,"Percolator.PEP":3.379e-09,"Percolator.SVMScore":3.381,"Precursor.Area":40354656,"Apex.RT..min.":15.1,"ptmRS..Binomial.Peptide.Score":344.79,"ptmRS..Isoform.Confidence.Probability":0.007,"ptmRS..Isoform.Group.Report":"y2+-H(3) O(4) P(14): 811.37","ptmRS..Best.Site.Probabilities":"S13(Phospho): 99.31","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.7; S(13): 99.3; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (O4)","Search.ID":"O","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"GVFGYGFEEPSAIQQR","Annotated.Sequence":"[R].GVFGYGFEEPSAIQQR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.5505,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":595.62411,"Contaminant":"False","Aligned.m.z..Da.":595.62412,"MH...Da.":1784.85778,"Theo..MH...Da.":1784.86022,"DeltaM..ppm.":-1.37,"Deltam.z..Da.":-0.00081,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":401091.8125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":30.43906,"Ion.Inject.Time..ms.":22,"RT..min.":15.044,"First.Scan":14906,"Last.Scan":14906,"Master.Scan.s.":14895,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_04.raw","File.ID":"F22","Quan.Info":"","Peptides.Matched":1380,"XCorr":1.98,"X..Protein.Groups":1,"Percolator.q.Value":0.0004,"Percolator.PEP":0.00003,"Percolator.SVMScore":1.676,"Precursor.Area":265709.34375,"Apex.RT..min.":15.03,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (O4)","Search.ID":"O","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"GVFGYGFEEPSAIQQR","Annotated.Sequence":"[R].GVFGYGFEEPSAIQQR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6208,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":892.93275,"Contaminant":"False","Aligned.m.z..Da.":892.93276,"MH...Da.":1784.85824,"Theo..MH...Da.":1784.86022,"DeltaM..ppm.":-1.11,"Deltam.z..Da.":-0.00099,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1089655.875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":49.94882,"Ion.Inject.Time..ms.":22,"RT..min.":15.058,"First.Scan":14922,"Last.Scan":14922,"Master.Scan.s.":14921,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_04.raw","File.ID":"F22","Quan.Info":"","Peptides.Matched":1382,"XCorr":2.69,"X..Protein.Groups":1,"Percolator.q.Value":0.0004,"Percolator.PEP":7.274e-08,"Percolator.SVMScore":2.803,"Precursor.Area":974324.125,"Apex.RT..min.":15.04,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (O4)","Search.ID":"O","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"GVFGYGFEEPSAIQQR","Annotated.Sequence":"[R].GVFGYGFEEPSAIQQR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6624,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":892.93195,"Contaminant":"False","Aligned.m.z..Da.":892.93196,"MH...Da.":1784.85662,"Theo..MH...Da.":1784.86022,"DeltaM..ppm.":-2.02,"Deltam.z..Da.":-0.0018,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":523298.46875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":26.96502,"Ion.Inject.Time..ms.":22,"RT..min.":15.0213,"First.Scan":14876,"Last.Scan":14876,"Master.Scan.s.":14869,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_04.raw","File.ID":"F22","Quan.Info":"","Peptides.Matched":1304,"XCorr":3.88,"X..Protein.Groups":1,"Percolator.q.Value":0.0004,"Percolator.PEP":2.115e-10,"Percolator.SVMScore":3.902,"Precursor.Area":974324.125,"Apex.RT..min.":15.04,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (O4)","Search.ID":"O","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":5,"DeltaScore":0.0584,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40581,"Contaminant":"False","Aligned.m.z..Da.":967.40579,"MH...Da.":2900.20287,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":1.22,"Deltam.z..Da.":0.00118,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":7531391.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":29.79997,"Ion.Inject.Time..ms.":22,"RT..min.":15.1622,"First.Scan":15054,"Last.Scan":15054,"Master.Scan.s.":15051,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_04.raw","File.ID":"F22","Quan.Info":"","Peptides.Matched":2537,"XCorr":5.99,"X..Protein.Groups":1,"Percolator.q.Value":0.0004,"Percolator.PEP":3.163e-12,"Percolator.SVMScore":4.694,"Precursor.Area":40354656,"Apex.RT..min.":15.1,"ptmRS..Binomial.Peptide.Score":657.83,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(13): 1413.58; b1+-H(3) O(4) P(14): 1528.61; b1+-H(3) O(4) P(15): 1642.65; b1+-H(3) O(4) P(16): 1771.69; y1+-H(3) O(4) P(15): 1750.78; y1+-H(3) O(4) P(14): 1621.73; y1+-H(3) O(4) P(13): 1458.67; b2+-H(3) O(4) P(16): 886.35; b2+-H(3) O(4) P(17): 950.38; b2+-H(3) O(4) P(18): 1014.90; y2+-H(3) O(4) P(14): 811.37; b1+(12): 1344.56; y1+(13): 1556.65","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (O4)","Search.ID":"O","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":5,"DeltaScore":0.0633,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40373,"Contaminant":"False","Aligned.m.z..Da.":967.40371,"MH...Da.":2900.19662,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":-0.93,"Deltam.z..Da.":-0.0009,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":11061221,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":2.93513,"Ion.Inject.Time..ms.":10.124,"RT..min.":15.1448,"First.Scan":15033,"Last.Scan":15033,"Master.Scan.s.":15025,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_04.raw","File.ID":"F22","Quan.Info":"","Peptides.Matched":2344,"XCorr":7.43,"X..Protein.Groups":1,"Percolator.q.Value":0.0004,"Percolator.PEP":2.574e-15,"Percolator.SVMScore":6.21,"Precursor.Area":40354656,"Apex.RT..min.":15.1,"ptmRS..Binomial.Peptide.Score":735.43,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(13): 1413.58; b1+-H(3) O(4) P(14): 1528.61; b1+-H(3) O(4) P(15): 1642.65; b1+-H(3) O(4) P(16): 1771.69; y1+-H(3) O(4) P(15): 1750.78; y1+-H(3) O(4) P(14): 1621.73; y1+-H(3) O(4) P(13): 1458.67; b2+-H(3) O(4) P(16): 886.35; b2+-H(3) O(4) P(18): 1014.90; y2+-H(3) O(4) P(14): 811.37; b1+(12): 1344.56; y1+(13): 1556.65","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (O4)","Search.ID":"O","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"AIMPIIEGHDVLAQAQSGTGK","Annotated.Sequence":"[R].AImPIIEGHDVLAQAQSGTGK.[T]","Modifications":"M3(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.666,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":718.04075,"Contaminant":"False","Aligned.m.z..Da.":718.04091,"MH...Da.":2152.1077,"Theo..MH...Da.":2152.10668,"DeltaM..ppm.":0.48,"Deltam.z..Da.":0.00034,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":805788.0625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":18.0507,"Ion.Inject.Time..ms.":22,"RT..min.":12.753,"First.Scan":11950,"Last.Scan":11950,"Master.Scan.s.":11938,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_04.raw","File.ID":"F22","Quan.Info":"","Peptides.Matched":971,"XCorr":4.73,"X..Protein.Groups":1,"Percolator.q.Value":0.0004,"Percolator.PEP":2.828e-11,"Percolator.SVMScore":4.281,"Precursor.Area":1102503.5,"Apex.RT..min.":12.77,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (O4)","Search.ID":"O","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"HSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[R].HSDSySENETNHTNVPISSTGGTNNK.[T]","Modifications":"Y5(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":0,"Charge":4,"Original.Precursor.Charge":4,"DeltaScore":0.0035,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":718.30173,"Contaminant":"False","Aligned.m.z..Da.":718.30179,"MH...Da.":2870.18512,"Theo..MH...Da.":2870.18608,"DeltaM..ppm.":-0.33,"Deltam.z..Da.":-0.00024,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":4009143.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":1.52692,"Ion.Inject.Time..ms.":22,"RT..min.":7.6478,"First.Scan":5429,"Last.Scan":5429,"Master.Scan.s.":5425,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_04.raw","File.ID":"F22","Quan.Info":"","Peptides.Matched":2458,"XCorr":5.71,"X..Protein.Groups":1,"Percolator.q.Value":0.0004,"Percolator.PEP":1.093e-08,"Percolator.SVMScore":3.16,"Precursor.Area":5482359,"Apex.RT..min.":7.66,"ptmRS..Binomial.Peptide.Score":307.64,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(9): 1031.37; b1+-H(3) O(4) P(10): 1132.42; b1+-H(3) O(4) P(11): 1246.46; b2+-H(3) O(4) P(7): 394.65; b2+-H(3) O(4) P(13): 742.79; b2+-H(3) O(4) P(14): 799.81; b2+-H(3) O(4) P(15): 849.34; b2+-H(3) O(4) P(17): 954.41; b1+(4): 427.16","ptmRS..Best.Site.Probabilities":"S6(Phospho): 99.38","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(4): 0.6; Y(5): 0.0; S(6): 99.4; T(10): 0.0; T(13): 0.0; S(18): 0.0; S(19): 0.0; T(20): 0.0; T(23): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (O4)","Search.ID":"O","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"HSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[R].HSDSYsENETNHTNVPISSTGGTNNK.[T]","Modifications":"S6(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.008,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":957.39999,"Contaminant":"False","Aligned.m.z..Da.":957.40006,"MH...Da.":2870.18544,"Theo..MH...Da.":2870.18608,"DeltaM..ppm.":-0.22,"Deltam.z..Da.":-0.00021,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":5735898,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":7.6472,"First.Scan":5428,"Last.Scan":5428,"Master.Scan.s.":5425,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_04.raw","File.ID":"F22","Quan.Info":"","Peptides.Matched":2441,"XCorr":6.28,"X..Protein.Groups":1,"Percolator.q.Value":0.0004,"Percolator.PEP":6.796e-13,"Percolator.SVMScore":4.983,"Precursor.Area":9460434,"Apex.RT..min.":7.66,"ptmRS..Binomial.Peptide.Score":579.19,"ptmRS..Isoform.Confidence.Probability":0.006,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(4): 409.15; b1+-H(3) O(4) P(5): 572.21; b1+-H(3) O(4) P(6): 659.24; b1+-H(3) O(4) P(10): 1132.42; b1+-H(3) O(4) P(11): 1246.46; b1+-H(3) O(4) P(12): 1383.52; b1+-H(3) O(4) P(15): 1697.68; b2+-H(3) O(4) P(12): 692.26; b2+-H(3) O(4) P(13): 742.79; b2+-H(3) O(4) P(14): 799.81; b2+-H(3) O(4) P(15): 849.34; b2+-H(3) O(4) P(17): 954.41; y2+-H(3) O(4) P(25): 1318.08; y2+-H(3) O(4) P(24): 1274.56; y2+-H(3) O(4) P(23): 1217.05","ptmRS..Best.Site.Probabilities":"S4(Phospho): 99.4","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(4): 99.4; Y(5): 0.0; S(6): 0.6; T(10): 0.0; T(13): 0.0; S(18): 0.0; S(19): 0.0; T(20): 0.0; T(23): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (O4)","Search.ID":"O","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MGSINNNFNTNNNSNTDLDRDWK","Annotated.Sequence":"[-].mGSINNNFNTNNNsNTDLDRDWK.[T]","Modifications":"N-Term(Prot)(Met-loss); S14(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P39517","Master.Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","Protein.Accessions":"P39517","Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0503,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":878.37119,"Contaminant":"False","Aligned.m.z..Da.":878.37129,"MH...Da.":2633.09901,"Theo..MH...Da.":2633.10123,"DeltaM..ppm.":-0.84,"Deltam.z..Da.":-0.00074,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2563118.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":11.2733,"First.Scan":9940,"Last.Scan":9940,"Master.Scan.s.":9936,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_04.raw","File.ID":"F22","Quan.Info":"","Peptides.Matched":2206,"XCorr":6.56,"X..Protein.Groups":1,"Percolator.q.Value":0.0004,"Percolator.PEP":1.004e-12,"Percolator.SVMScore":4.91,"Precursor.Area":3345656.75,"Apex.RT..min.":11.28,"ptmRS..Binomial.Peptide.Score":613.8,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(14): 1674.75; y1+-H(3) O(4) P(13): 1573.70; y1+-H(3) O(4) P(12): 1459.66; y1+-H(3) O(4) P(11): 1345.61; y1+-H(3) O(4) P(10): 1231.57; y2+-H(3) O(4) P(19): 1139.50; y2+-H(3) O(4) P(17): 1025.45; y2+-H(3) O(4) P(16): 968.43; y2+-H(3) O(4) P(15): 894.90; y2+-H(3) O(4) P(14): 837.88; y2+-H(3) O(4) P(13): 787.35; y2+-H(3) O(4) P(12): 730.33; y2+-H(3) O(4) P(11): 673.31; y2+-H(3) O(4) P(10): 616.29; y1+(9): 1162.55; y2+(9): 581.78","ptmRS..Best.Site.Probabilities":"S14(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(3): 0.0; T(10): 0.0; S(14): 100.0; T(16): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (O4)","Search.ID":"O","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LQETNPEEVPK","Annotated.Sequence":"[K].LQETNPEEVPK.[F]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5451,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":642.32763,"Contaminant":"False","Aligned.m.z..Da.":642.32767,"MH...Da.":1283.64799,"Theo..MH...Da.":1283.64777,"DeltaM..ppm.":0.17,"Deltam.z..Da.":0.00011,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3728487.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":1.70848,"Ion.Inject.Time..ms.":22,"RT..min.":7.6288,"First.Scan":5402,"Last.Scan":5402,"Master.Scan.s.":5399,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_04.raw","File.ID":"F22","Quan.Info":"","Peptides.Matched":526,"XCorr":2.66,"X..Protein.Groups":1,"Percolator.q.Value":0.0004,"Percolator.PEP":0.00003,"Percolator.SVMScore":1.66,"Precursor.Area":6422808.5,"Apex.RT..min.":7.65,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (O4)","Search.ID":"O","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KDELTLEGIK","Annotated.Sequence":"[K].KDELTLEGIK.[Q]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5178,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":573.32416,"Contaminant":"False","Aligned.m.z..Da.":573.32421,"MH...Da.":1145.64105,"Theo..MH...Da.":1145.64123,"DeltaM..ppm.":-0.15,"Deltam.z..Da.":-0.00009,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2434465.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":7.25852,"Ion.Inject.Time..ms.":22,"RT..min.":10.2517,"First.Scan":8654,"Last.Scan":8654,"Master.Scan.s.":8649,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_04.raw","File.ID":"F22","Quan.Info":"","Peptides.Matched":311,"XCorr":3.65,"X..Protein.Groups":1,"Percolator.q.Value":0.0004,"Percolator.PEP":1.113e-08,"Percolator.SVMScore":3.156,"Precursor.Area":2485202.5,"Apex.RT..min.":10.26,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (O4)","Search.ID":"O","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"APQALMLAPTR","Annotated.Sequence":"[K].APQALmLAPTR.[E]","Modifications":"M6(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7184,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":592.82611,"Contaminant":"False","Aligned.m.z..Da.":592.82615,"MH...Da.":1184.64494,"Theo..MH...Da.":1184.6456,"DeltaM..ppm.":-0.56,"Deltam.z..Da.":-0.00033,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1505640.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":10.23062,"Ion.Inject.Time..ms.":22,"RT..min.":10.1723,"First.Scan":8557,"Last.Scan":8557,"Master.Scan.s.":8545,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_04.raw","File.ID":"F22","Quan.Info":"","Peptides.Matched":327,"XCorr":3.09,"X..Protein.Groups":1,"Percolator.q.Value":0.0004,"Percolator.PEP":0.00002,"Percolator.SVMScore":1.772,"Precursor.Area":2654795.75,"Apex.RT..min.":10.19,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (O4)","Search.ID":"O","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"IQDPQMTGYVSTR","Annotated.Sequence":"[R].IQDPQmTGyVSTR.[Y]","Modifications":"M6(Oxidation); Y9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P32485","Master.Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","Protein.Accessions":"P32485","Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1051,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":796.34495,"Contaminant":"False","Aligned.m.z..Da.":796.34502,"MH...Da.":1591.68264,"Theo..MH...Da.":1591.6822,"DeltaM..ppm.":0.27,"Deltam.z..Da.":0.00022,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1519973.875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":38.30914,"Ion.Inject.Time..ms.":22,"RT..min.":7.7289,"First.Scan":5543,"Last.Scan":5543,"Master.Scan.s.":5542,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_04.raw","File.ID":"F22","Quan.Info":"","Peptides.Matched":1236,"XCorr":2.57,"X..Protein.Groups":1,"Percolator.q.Value":0.0004,"Percolator.PEP":4.119e-06,"Percolator.SVMScore":2.043,"Precursor.Area":5383366,"Apex.RT..min.":7.68,"ptmRS..Binomial.Peptide.Score":235.17,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(10): 1137.54; y1+-H(3) O(4) P(8): 912.42; b1+(7): 910.34","ptmRS..Best.Site.Probabilities":"T7(Phospho): 99.99","ptmRS..Phospho.Site.Probabilities":"T(7): 100.0; Y(9): 0.0; S(11): 0.0; T(12): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (O4)","Search.ID":"O","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"APQALMLAPTR","Annotated.Sequence":"[K].APQALMLAPTR.[E]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6894,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":584.82949,"Contaminant":"False","Aligned.m.z..Da.":584.82954,"MH...Da.":1168.6517,"Theo..MH...Da.":1168.65069,"DeltaM..ppm.":0.87,"Deltam.z..Da.":0.00051,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":624273.125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":68.51679,"Ion.Inject.Time..ms.":22,"RT..min.":11.6157,"First.Scan":10393,"Last.Scan":10393,"Master.Scan.s.":10391,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_04.raw","File.ID":"F22","Quan.Info":"","Peptides.Matched":202,"XCorr":2.64,"X..Protein.Groups":1,"Percolator.q.Value":0.0004,"Percolator.PEP":3.39e-06,"Percolator.SVMScore":2.08,"Precursor.Area":539600.9375,"Apex.RT..min.":11.61,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (V4)","Search.ID":"V","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MGSINNNFNTNNNSNTDLDR","Annotated.Sequence":"[-].mGSINNNFNTNNNsNTDLDR.[D]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S14(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P39517","Master.Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","Protein.Accessions":"P39517","Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.1179,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":749.31074,"Contaminant":"False","Aligned.m.z..Da.":749.31074,"MH...Da.":2245.91766,"Theo..MH...Da.":2245.91057,"DeltaM..ppm.":3.16,"Deltam.z..Da.":0.00236,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":358785.4375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":39.37783,"Ion.Inject.Time..ms.":22,"RT..min.":11.9844,"First.Scan":10947,"Last.Scan":10947,"Master.Scan.s.":10938,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_03.raw","File.ID":"F15","Quan.Info":"","Peptides.Matched":1466,"XCorr":2.29,"X..Protein.Groups":1,"Percolator.q.Value":0.0004,"Percolator.PEP":0.0012,"Percolator.SVMScore":0.899,"Precursor.Area":445571.4375,"Apex.RT..min.":11.99,"ptmRS..Binomial.Peptide.Score":199.94,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(9): 1030.45; y1+-H(3) O(4) P(8): 916.41; y1+-H(3) O(4) P(7): 802.37; b2+-H(3) O(4) P(19): 987.41; y2+-H(3) O(4) P(7): 401.69; y1+(6): 733.35; y1+(5): 619.30","ptmRS..Best.Site.Probabilities":"S14(Phospho): 99.98","ptmRS..Phospho.Site.Probabilities":"S(3): 0.0; T(10): 0.0; S(14): 100.0; T(16): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (V4)","Search.ID":"V","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"SFLTYIK","Annotated.Sequence":"[K].SFLTYIK.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6316,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":436.24968,"Contaminant":"False","Aligned.m.z..Da.":436.24972,"MH...Da.":871.49209,"Theo..MH...Da.":871.49238,"DeltaM..ppm.":-0.34,"Deltam.z..Da.":-0.00015,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":386022.1875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":13.6807,"First.Scan":13147,"Last.Scan":13147,"Master.Scan.s.":13135,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_03.raw","File.ID":"F15","Quan.Info":"","Peptides.Matched":273,"XCorr":1.52,"X..Protein.Groups":1,"Percolator.q.Value":0.0004,"Percolator.PEP":0.00149,"Percolator.SVMScore":0.857,"Precursor.Area":4173129.25,"Apex.RT..min.":13.71,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (V4)","Search.ID":"V","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LQQTAFDKK","Annotated.Sequence":"[R].LQQTAFDKK.[S]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":4,"DeltaScore":0.3333,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":360.20131,"Contaminant":"False","Aligned.m.z..Da.":360.20131,"MH...Da.":1078.58939,"Theo..MH...Da.":1078.58914,"DeltaM..ppm.":0.24,"Deltam.z..Da.":0.00009,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1760989.375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":69.09657,"Ion.Inject.Time..ms.":18.422,"RT..min.":5.485,"First.Scan":2239,"Last.Scan":2239,"Master.Scan.s.":2228,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_03.raw","File.ID":"F15","Quan.Info":"","Peptides.Matched":392,"XCorr":2.04,"X..Protein.Groups":1,"Percolator.q.Value":0.0004,"Percolator.PEP":0.00155,"Percolator.SVMScore":0.849,"Precursor.Area":1308901,"Apex.RT..min.":5.49,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AA4)","Search.ID":"AA","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DIFSNDELLSDAYDAK","Annotated.Sequence":"[K].DIFSNDELLSDAYDAK.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.5556,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":605.94892,"Contaminant":"False","Aligned.m.z..Da.":605.94873,"MH...Da.":1815.83222,"Theo..MH...Da.":1815.82831,"DeltaM..ppm.":2.15,"Deltam.z..Da.":0.0013,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":257259.78125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":7.60858,"Ion.Inject.Time..ms.":22,"RT..min.":17.0825,"First.Scan":17129,"Last.Scan":17129,"Master.Scan.s.":17126,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_04.raw","File.ID":"F10","Quan.Info":"","Peptides.Matched":1694,"XCorr":1.71,"X..Protein.Groups":1,"Percolator.q.Value":0.0004,"Percolator.PEP":0.0024,"Percolator.SVMScore":0.807,"Precursor.Area":215329.0625,"Apex.RT..min.":17.08,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AA4)","Search.ID":"AA","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ELALQIQK","Annotated.Sequence":"[R].ELALQIQK.[V]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.2156,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":471.78495,"Contaminant":"False","Aligned.m.z..Da.":471.78495,"MH...Da.":942.56262,"Theo..MH...Da.":942.56186,"DeltaM..ppm.":0.81,"Deltam.z..Da.":0.00038,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1204591,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":2.72485,"Ion.Inject.Time..ms.":22,"RT..min.":10.5802,"First.Scan":9036,"Last.Scan":9036,"Master.Scan.s.":9032,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_04.raw","File.ID":"F10","Quan.Info":"","Peptides.Matched":292,"XCorr":2.18,"X..Protein.Groups":1,"Percolator.q.Value":0.0004,"Percolator.PEP":0.0021,"Percolator.SVMScore":0.833,"Precursor.Area":995107.75,"Apex.RT..min.":10.59,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AA4)","Search.ID":"AA","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"NQQLEDEIEK","Annotated.Sequence":"[K].NQQLEDEIEK.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1083,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":623.30029,"Contaminant":"False","Aligned.m.z..Da.":623.30033,"MH...Da.":1245.5933,"Theo..MH...Da.":1245.59574,"DeltaM..ppm.":-1.96,"Deltam.z..Da.":-0.00122,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":863868.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":82.5757,"Ion.Inject.Time..ms.":22,"RT..min.":8.568,"First.Scan":6643,"Last.Scan":6643,"Master.Scan.s.":6640,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_04.raw","File.ID":"F10","Quan.Info":"NoneMonoisotopic","Peptides.Matched":772,"XCorr":1.2,"X..Protein.Groups":1,"Percolator.q.Value":0.0004,"Percolator.PEP":0.00208,"Percolator.SVMScore":0.835,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (R4)","Search.ID":"R","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"GVFGYGFEEPSAIQQR","Annotated.Sequence":"[R].GVFGYGFEEPSAIQQR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.4142,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":892.93399,"Contaminant":"False","Aligned.m.z..Da.":892.93412,"MH...Da.":1784.86071,"Theo..MH...Da.":1784.86022,"DeltaM..ppm.":0.27,"Deltam.z..Da.":0.00024,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":492094.5625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":61.24783,"Ion.Inject.Time..ms.":22,"RT..min.":15.0516,"First.Scan":14668,"Last.Scan":14668,"Master.Scan.s.":14657,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_01.raw","File.ID":"F19","Quan.Info":"","Peptides.Matched":1370,"XCorr":1.69,"X..Protein.Groups":1,"Percolator.q.Value":0.00041,"Percolator.PEP":0.00033,"Percolator.SVMScore":1.235,"Precursor.Area":934604.8125,"Apex.RT..min.":15.02,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (R4)","Search.ID":"R","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"GVFGYGFEEPSAIQQR","Annotated.Sequence":"[R].GVFGYGFEEPSAIQQR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.5874,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":595.62487,"Contaminant":"False","Aligned.m.z..Da.":595.62496,"MH...Da.":1784.86007,"Theo..MH...Da.":1784.86022,"DeltaM..ppm.":-0.09,"Deltam.z..Da.":-0.00005,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":348944.90625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":18.42775,"Ion.Inject.Time..ms.":22,"RT..min.":15.0286,"First.Scan":14639,"Last.Scan":14639,"Master.Scan.s.":14631,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_01.raw","File.ID":"F19","Quan.Info":"","Peptides.Matched":1260,"XCorr":2.06,"X..Protein.Groups":1,"Percolator.q.Value":0.00041,"Percolator.PEP":0.00022,"Percolator.SVMScore":1.308,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (R4)","Search.ID":"R","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.479,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40857,"Contaminant":"False","Aligned.m.z..Da.":967.40858,"MH...Da.":2900.21116,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":4.08,"Deltam.z..Da.":0.00395,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":94369.60938,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":18.7836,"First.Scan":19410,"Last.Scan":19410,"Master.Scan.s.":19400,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_01.raw","File.ID":"F19","Quan.Info":"","Peptides.Matched":2631,"XCorr":1.67,"X..Protein.Groups":1,"Percolator.q.Value":0.00041,"Percolator.PEP":0.00058,"Percolator.SVMScore":1.125,"Precursor.Area":160019.60938,"Apex.RT..min.":18.86,"ptmRS..Binomial.Peptide.Score":251.21,"ptmRS..Isoform.Confidence.Probability":0.5,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"Y12(Phospho): 50; S13(Phospho): 50","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 50.0; S(13): 50.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (R4)","Search.ID":"R","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSSPLHRLSPTGK","Annotated.Sequence":"[R].mSsPLHRLsPTGK.[S]","Modifications":"M1(Oxidation); S3(Phospho); S9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.4661,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":793.84411,"Contaminant":"False","Aligned.m.z..Da.":793.84416,"MH...Da.":1586.68095,"Theo..MH...Da.":1586.67977,"DeltaM..ppm.":0.75,"Deltam.z..Da.":0.00059,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1551557,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":13.13076,"Ion.Inject.Time..ms.":22,"RT..min.":7.7526,"First.Scan":5568,"Last.Scan":5568,"Master.Scan.s.":5567,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_01.raw","File.ID":"F19","Quan.Info":"","Peptides.Matched":1071,"XCorr":2.21,"X..Protein.Groups":1,"Percolator.q.Value":0.00041,"Percolator.PEP":0.0005,"Percolator.SVMScore":1.154,"Precursor.Area":1634037.75,"Apex.RT..min.":7.74,"ptmRS..Binomial.Peptide.Score":278.87,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(3): 304.10; y2+-H(3) O(4) P(11): 627.82; y2+- -2H(3) O(4) P -2H(3) O(4) P(11): 578.83; b1+(2): 235.07","ptmRS..Best.Site.Probabilities":"S3(Phospho): 100; S9(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(3): 100.0; S(9): 100.0; T(11): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (R4)","Search.ID":"R","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSSPLHRLSPTGK","Annotated.Sequence":"[R].mSsPLHRLsPTGK.[S]","Modifications":"M1(Oxidation); S3(Phospho); S9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.4603,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":529.56464,"Contaminant":"False","Aligned.m.z..Da.":529.56467,"MH...Da.":1586.67938,"Theo..MH...Da.":1586.67977,"DeltaM..ppm.":-0.25,"Deltam.z..Da.":-0.00013,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3313251.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":46.76623,"Ion.Inject.Time..ms.":22,"RT..min.":7.7185,"First.Scan":5519,"Last.Scan":5519,"Master.Scan.s.":5515,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_01.raw","File.ID":"F19","Quan.Info":"","Peptides.Matched":1016,"XCorr":3.65,"X..Protein.Groups":1,"Percolator.q.Value":0.00041,"Percolator.PEP":0.0008,"Percolator.SVMScore":1.066,"Precursor.Area":5320344,"Apex.RT..min.":7.74,"ptmRS..Binomial.Peptide.Score":184.03,"ptmRS..Isoform.Confidence.Probability":0.991,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(8): 877.50; y2+-H(3) O(4) P(10): 544.32; y2+-H(3) O(4) P(8): 439.25; y1+(4): 402.23","ptmRS..Best.Site.Probabilities":"S3(Phospho): 100; S9(Phospho): 99.1","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(3): 100.0; S(9): 99.1; T(11): 0.9"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (R4)","Search.ID":"R","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"IGNNELLHR","Annotated.Sequence":"[K].IGNNELLHR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q03558","Master.Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","Protein.Accessions":"Q03558","Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.5506,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":355.86494,"Contaminant":"False","Aligned.m.z..Da.":355.86496,"MH...Da.":1065.58029,"Theo..MH...Da.":1065.57997,"DeltaM..ppm.":0.3,"Deltam.z..Da.":0.00011,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1545225.125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":16.44045,"Ion.Inject.Time..ms.":22,"RT..min.":7.5821,"First.Scan":5316,"Last.Scan":5316,"Master.Scan.s.":5307,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_01.raw","File.ID":"F19","Quan.Info":"","Peptides.Matched":300,"XCorr":2.47,"X..Protein.Groups":1,"Percolator.q.Value":0.00041,"Percolator.PEP":0.00026,"Percolator.SVMScore":1.281,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (R4)","Search.ID":"R","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"TAVFYGGTPISK","Annotated.Sequence":"[K].TAVFYGGTPISK.[D]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5608,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":620.83183,"Contaminant":"False","Aligned.m.z..Da.":620.8319,"MH...Da.":1240.65638,"Theo..MH...Da.":1240.65721,"DeltaM..ppm.":-0.67,"Deltam.z..Da.":-0.00042,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":887469.125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":66.33852,"Ion.Inject.Time..ms.":22,"RT..min.":11.1337,"First.Scan":9644,"Last.Scan":9644,"Master.Scan.s.":9636,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_01.raw","File.ID":"F19","Quan.Info":"","Peptides.Matched":418,"XCorr":2.55,"X..Protein.Groups":1,"Percolator.q.Value":0.00041,"Percolator.PEP":0.0006,"Percolator.SVMScore":1.121,"Precursor.Area":579283.8125,"Apex.RT..min.":11.13,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (R4)","Search.ID":"R","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KDELTLEGIK","Annotated.Sequence":"[K].KDELTLEGIK.[Q]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.5961,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":382.55169,"Contaminant":"False","Aligned.m.z..Da.":382.55172,"MH...Da.":1145.64054,"Theo..MH...Da.":1145.64123,"DeltaM..ppm.":-0.61,"Deltam.z..Da.":-0.00023,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1835422,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":5.49736,"Ion.Inject.Time..ms.":22,"RT..min.":10.2413,"First.Scan":8540,"Last.Scan":8540,"Master.Scan.s.":8531,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_01.raw","File.ID":"F19","Quan.Info":"","Peptides.Matched":314,"XCorr":3.07,"X..Protein.Groups":1,"Percolator.q.Value":0.00041,"Percolator.PEP":0.00018,"Percolator.SVMScore":1.344,"Precursor.Area":1835538.375,"Apex.RT..min.":10.25,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AB4)","Search.ID":"AB","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ELALQIQK","Annotated.Sequence":"[R].ELALQIQK.[V]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.2301,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":471.78478,"Contaminant":"False","Aligned.m.z..Da.":471.7848,"MH...Da.":942.5623,"Theo..MH...Da.":942.56186,"DeltaM..ppm.":0.47,"Deltam.z..Da.":0.00022,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1004452,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":6.05016,"Ion.Inject.Time..ms.":22,"RT..min.":10.589,"First.Scan":9059,"Last.Scan":9059,"Master.Scan.s.":9054,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_03.raw","File.ID":"F9","Quan.Info":"","Peptides.Matched":292,"XCorr":2.26,"X..Protein.Groups":1,"Percolator.q.Value":0.00042,"Percolator.PEP":0.00148,"Percolator.SVMScore":0.869,"Precursor.Area":765769.375,"Apex.RT..min.":10.59,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AB4)","Search.ID":"AB","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LQETNPEEVPK","Annotated.Sequence":"[K].LQETNPEEVPK.[F]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.4961,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":642.32759,"Contaminant":"False","Aligned.m.z..Da.":642.32763,"MH...Da.":1283.64791,"Theo..MH...Da.":1283.64777,"DeltaM..ppm.":0.11,"Deltam.z..Da.":0.00007,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1631591.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":3.32242,"Ion.Inject.Time..ms.":22,"RT..min.":7.6519,"First.Scan":5510,"Last.Scan":5510,"Master.Scan.s.":5505,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_03.raw","File.ID":"F9","Quan.Info":"","Peptides.Matched":526,"XCorr":2.58,"X..Protein.Groups":1,"Percolator.q.Value":0.00042,"Percolator.PEP":0.00178,"Percolator.SVMScore":0.836,"Precursor.Area":2336846.5,"Apex.RT..min.":7.66,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (N4)","Search.ID":"N","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"LEAGLSDSK","Annotated.Sequence":"[K].LEAGLsDSK.[Q]","Modifications":"S6(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.0094,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":500.22347,"Contaminant":"False","Aligned.m.z..Da.":500.22362,"MH...Da.":999.43967,"Theo..MH...Da.":999.43943,"DeltaM..ppm.":0.24,"Deltam.z..Da.":0.00012,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2880568.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":67.25436,"Ion.Inject.Time..ms.":19.295,"RT..min.":7.6824,"First.Scan":5897,"Last.Scan":5897,"Master.Scan.s.":5892,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_05.raw","File.ID":"F23","Quan.Info":"","Peptides.Matched":652,"XCorr":2.12,"X..Protein.Groups":1,"Percolator.q.Value":0.00042,"Percolator.PEP":0.00463,"Percolator.SVMScore":0.729,"Precursor.Area":2096116.375,"Apex.RT..min.":7.68,"ptmRS..Binomial.Peptide.Score":175.39,"ptmRS..Isoform.Confidence.Probability":0.995,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(8): 788.38; y1+-H(3) O(4) P(7): 659.34; y1+-H(3) O(4) P(6): 588.30; y1+(3): 349.17; y1+(2): 234.14","ptmRS..Best.Site.Probabilities":"S6(Phospho): 99.53","ptmRS..Phospho.Site.Probabilities":"S(6): 99.5; S(8): 0.5"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (N4)","Search.ID":"N","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KFQDDTLNR","Annotated.Sequence":"[K].KFQDDtLNR.[V]","Modifications":"T6(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.62,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":608.77321,"Contaminant":"False","Aligned.m.z..Da.":608.77326,"MH...Da.":1216.53915,"Theo..MH...Da.":1216.53579,"DeltaM..ppm.":2.76,"Deltam.z..Da.":0.00168,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":908374.8125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":43.974,"Ion.Inject.Time..ms.":22,"RT..min.":6.2231,"First.Scan":3811,"Last.Scan":3811,"Master.Scan.s.":3799,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_05.raw","File.ID":"F23","Quan.Info":"","Peptides.Matched":672,"XCorr":2.5,"X..Protein.Groups":1,"Percolator.q.Value":0.00042,"Percolator.PEP":0.00252,"Percolator.SVMScore":0.847,"Precursor.Area":2179926.5,"Apex.RT..min.":6.24,"ptmRS..Binomial.Peptide.Score":322.46,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"T6(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"T(6): 100.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (J4)","Search.ID":"J","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"TAVFYGGTPISK","Annotated.Sequence":"[K].TAVFYGGTPISK.[D]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5144,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":620.83147,"Contaminant":"False","Aligned.m.z..Da.":620.83148,"MH...Da.":1240.65565,"Theo..MH...Da.":1240.65721,"DeltaM..ppm.":-1.26,"Deltam.z..Da.":-0.00078,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":570118.3125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":82.78134,"Ion.Inject.Time..ms.":22,"RT..min.":11.1243,"First.Scan":9750,"Last.Scan":9750,"Master.Scan.s.":9743,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_03.raw","File.ID":"F27","Quan.Info":"","Peptides.Matched":397,"XCorr":2.43,"X..Protein.Groups":1,"Percolator.q.Value":0.00042,"Percolator.PEP":0.00038,"Percolator.SVMScore":1.448,"Precursor.Area":790030.3125,"Apex.RT..min.":11.14,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (J4)","Search.ID":"J","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ILISTDLLAR","Annotated.Sequence":"[R].ILISTDLLAR.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5153,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":557.84549,"Contaminant":"False","Aligned.m.z..Da.":557.84545,"MH...Da.":1114.6837,"Theo..MH...Da.":1114.68304,"DeltaM..ppm.":0.59,"Deltam.z..Da.":0.00033,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1966033.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":23.65108,"Ion.Inject.Time..ms.":22,"RT..min.":15.5853,"First.Scan":15597,"Last.Scan":15597,"Master.Scan.s.":15593,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_03.raw","File.ID":"F27","Quan.Info":"","Peptides.Matched":124,"XCorr":2.62,"X..Protein.Groups":1,"Percolator.q.Value":0.00042,"Percolator.PEP":0.00038,"Percolator.SVMScore":1.457,"Precursor.Area":2437072.75,"Apex.RT..min.":15.6,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (J4)","Search.ID":"J","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"SQMSVELDDDADLDDELAQLK","Annotated.Sequence":"[R].SQmsVELDDDADLDDELAQLK.[G]","Modifications":"M3(Oxidation); S4(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q08421","Master.Protein.Descriptions":"Enhancer of translation termination 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ETT1 PE=1 SV=1","Protein.Accessions":"Q08421","Protein.Descriptions":"Enhancer of translation termination 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ETT1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0906,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":816.01449,"Contaminant":"False","Aligned.m.z..Da.":816.01396,"MH...Da.":2446.02891,"Theo..MH...Da.":2446.02149,"DeltaM..ppm.":3.04,"Deltam.z..Da.":0.00248,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":305167.34375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":15.70786,"Ion.Inject.Time..ms.":22,"RT..min.":17.4833,"First.Scan":18012,"Last.Scan":18012,"Master.Scan.s.":18010,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_03.raw","File.ID":"F27","Quan.Info":"","Peptides.Matched":2222,"XCorr":2.98,"X..Protein.Groups":1,"Percolator.q.Value":0.00042,"Percolator.PEP":0.00056,"Percolator.SVMScore":1.321,"ptmRS..Binomial.Peptide.Score":279.83,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(5): 531.22; b1+-H(3) O(4) P(6): 660.27; b1+-H(3) O(4) P(7): 773.35; b1+-H(3) O(4) P(10): 1118.43; b1+(2): 216.10; b1+(3): 363.13","ptmRS..Best.Site.Probabilities":"S4(Phospho): 99.99","ptmRS..Phospho.Site.Probabilities":"S(1): 0.0; S(4): 100.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (J4)","Search.ID":"J","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"LEAGLSDSK","Annotated.Sequence":"[K].LEAGLsDSK.[Q]","Modifications":"S6(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.0226,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":500.22389,"Contaminant":"False","Aligned.m.z..Da.":500.22387,"MH...Da.":999.4405,"Theo..MH...Da.":999.43943,"DeltaM..ppm.":1.07,"Deltam.z..Da.":0.00053,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3459408.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":12.88772,"Ion.Inject.Time..ms.":22,"RT..min.":7.5789,"First.Scan":5353,"Last.Scan":5353,"Master.Scan.s.":5349,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_03.raw","File.ID":"F27","Quan.Info":"","Peptides.Matched":749,"XCorr":2.65,"X..Protein.Groups":1,"Percolator.q.Value":0.00042,"Percolator.PEP":0.00065,"Percolator.SVMScore":1.272,"Precursor.Area":3517142,"Apex.RT..min.":7.59,"ptmRS..Binomial.Peptide.Score":269.15,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(8): 788.38; y1+-H(3) O(4) P(7): 659.34; y1+-H(3) O(4) P(6): 588.30; y1+-H(3) O(4) P(5): 531.28; y1+-H(3) O(4) P(4): 418.19; y2+-H(3) O(4) P(6): 294.65; y1+(3): 349.17; y1+(2): 234.14","ptmRS..Best.Site.Probabilities":"S6(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(6): 100.0; S(8): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (J4)","Search.ID":"J","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"NHQLEEEIEK","Annotated.Sequence":"[K].NHQLEEEIEK.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.359,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":634.81146,"Contaminant":"False","Aligned.m.z..Da.":634.8114,"MH...Da.":1268.61565,"Theo..MH...Da.":1268.61172,"DeltaM..ppm.":3.1,"Deltam.z..Da.":0.00196,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":528534.0625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":76.51006,"Ion.Inject.Time..ms.":22,"RT..min.":7.187,"First.Scan":4733,"Last.Scan":4733,"Master.Scan.s.":4725,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_03.raw","File.ID":"F27","Quan.Info":"","Peptides.Matched":838,"XCorr":1.56,"X..Protein.Groups":1,"Percolator.q.Value":0.00042,"Percolator.PEP":0.0004,"Percolator.SVMScore":1.435,"Precursor.Area":647062.5,"Apex.RT..min.":7.19,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (J4)","Search.ID":"J","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"GVFGYGFEEPSAIQQR","Annotated.Sequence":"[R].GVFGYGFEEPSAIQQR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.64,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":595.62454,"Contaminant":"False","Aligned.m.z..Da.":595.62458,"MH...Da.":1784.85906,"Theo..MH...Da.":1784.86022,"DeltaM..ppm.":-0.65,"Deltam.z..Da.":-0.00039,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":502566.53125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":21.73527,"Ion.Inject.Time..ms.":22,"RT..min.":15.0372,"First.Scan":14900,"Last.Scan":14900,"Master.Scan.s.":14891,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_03.raw","File.ID":"F27","Quan.Info":"","Peptides.Matched":1282,"XCorr":2.5,"X..Protein.Groups":1,"Percolator.q.Value":0.00042,"Percolator.PEP":0.00047,"Percolator.SVMScore":1.377,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (J4)","Search.ID":"J","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"EDGTTPFVAIWK","Annotated.Sequence":"[R].EDGTTPFVAIWK.[H]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.663,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":682.34702,"Contaminant":"False","Aligned.m.z..Da.":682.34679,"MH...Da.":1363.68677,"Theo..MH...Da.":1363.68924,"DeltaM..ppm.":-1.82,"Deltam.z..Da.":-0.00124,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":472802.5625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":16.904,"First.Scan":17268,"Last.Scan":17268,"Master.Scan.s.":17256,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_03.raw","File.ID":"F27","Quan.Info":"","Peptides.Matched":657,"XCorr":1.84,"X..Protein.Groups":1,"Percolator.q.Value":0.00042,"Percolator.PEP":0.00064,"Percolator.SVMScore":1.279,"Precursor.Area":291066.75,"Apex.RT..min.":16.91,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (J4)","Search.ID":"J","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DIFSNDELLSDAYDAK","Annotated.Sequence":"[K].DIFSNDELLSDAYDAK.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.6281,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":605.94787,"Contaminant":"False","Aligned.m.z..Da.":605.94762,"MH...Da.":1815.82907,"Theo..MH...Da.":1815.82831,"DeltaM..ppm.":0.42,"Deltam.z..Da.":0.00025,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":360459.28125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":2.56497,"Ion.Inject.Time..ms.":22,"RT..min.":17.0816,"First.Scan":17494,"Last.Scan":17494,"Master.Scan.s.":17490,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_03.raw","File.ID":"F27","Quan.Info":"","Peptides.Matched":1570,"XCorr":2.42,"X..Protein.Groups":1,"Percolator.q.Value":0.00042,"Percolator.PEP":0.0003,"Percolator.SVMScore":1.54,"Precursor.Area":697926.8125,"Apex.RT..min.":17.1,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (J4)","Search.ID":"J","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"IQDPQMTGYVSTR","Annotated.Sequence":"[R].IQDPQmtGYVsTR.[Y]","Modifications":"M6(Oxidation); T7(Phospho); S11(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P32485","Master.Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","Protein.Accessions":"P32485","Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.0493,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":836.32768,"Contaminant":"False","Aligned.m.z..Da.":836.32768,"MH...Da.":1671.64809,"Theo..MH...Da.":1671.64853,"DeltaM..ppm.":-0.26,"Deltam.z..Da.":-0.00022,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3266212.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":10.7345,"Ion.Inject.Time..ms.":22,"RT..min.":7.7381,"First.Scan":5585,"Last.Scan":5585,"Master.Scan.s.":5583,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_03.raw","File.ID":"F27","Quan.Info":"","Peptides.Matched":768,"XCorr":2.84,"X..Protein.Groups":1,"Percolator.q.Value":0.00042,"Percolator.PEP":0.00049,"Percolator.SVMScore":1.364,"Precursor.Area":3070870.5,"Apex.RT..min.":7.75,"ptmRS..Binomial.Peptide.Score":275.56,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(11): 1332.53; y1+-H(3) O(4) P(10): 1217.50; y1+-H(3) O(4) P(8): 992.39; y1+-H(3) O(4) P(7): 845.36; y2+-H(3) O(4) P(10): 609.25; y1+(4): 462.27; y1+(3): 363.20; y1+(2): 276.17","ptmRS..Best.Site.Probabilities":"T7(Phospho): 100; Y9(Phospho): 99.5","ptmRS..Phospho.Site.Probabilities":"T(7): 100.0; Y(9): 99.5; S(11): 0.0; T(12): 0.5"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (J4)","Search.ID":"J","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSSPLHRLSPTGK","Annotated.Sequence":"[R].mSsPLHRLsPTGK.[S]","Modifications":"M1(Oxidation); S3(Phospho); S9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.3975,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":793.84351,"Contaminant":"False","Aligned.m.z..Da.":793.84351,"MH...Da.":1586.67975,"Theo..MH...Da.":1586.67977,"DeltaM..ppm.":-0.02,"Deltam.z..Da.":-1e-05,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3293929,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":17.52372,"Ion.Inject.Time..ms.":22,"RT..min.":7.7333,"First.Scan":5580,"Last.Scan":5580,"Master.Scan.s.":5570,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_03.raw","File.ID":"F27","Quan.Info":"","Peptides.Matched":973,"XCorr":2.44,"X..Protein.Groups":1,"Percolator.q.Value":0.00042,"Percolator.PEP":0.00077,"Percolator.SVMScore":1.216,"Precursor.Area":2640847.25,"Apex.RT..min.":7.73,"ptmRS..Binomial.Peptide.Score":320.15,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(8): 877.50; y1+-H(3) O(4) P(5): 471.26; y2+-H(3) O(4) P(11): 627.82; y2+-H(3) O(4) P(10): 544.32; b1+- -2H(3) O(4) P -2H(3) O(4) P(9): 989.50; y1+(4): 402.23; y1+(3): 305.18","ptmRS..Best.Site.Probabilities":"S3(Phospho): 100; S9(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(3): 100.0; S(9): 100.0; T(11): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (J4)","Search.ID":"J","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSSPLHRLSPTGK","Annotated.Sequence":"[R].mSsPLHRLsPTGK.[S]","Modifications":"M1(Oxidation); S3(Phospho); S9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.4485,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":529.56448,"Contaminant":"False","Aligned.m.z..Da.":529.56448,"MH...Da.":1586.67889,"Theo..MH...Da.":1586.67977,"DeltaM..ppm.":-0.55,"Deltam.z..Da.":-0.00029,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3653521,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":13.18141,"Ion.Inject.Time..ms.":22,"RT..min.":7.7121,"First.Scan":5549,"Last.Scan":5549,"Master.Scan.s.":5544,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_03.raw","File.ID":"F27","Quan.Info":"","Peptides.Matched":978,"XCorr":3.59,"X..Protein.Groups":1,"Percolator.q.Value":0.00042,"Percolator.PEP":0.00048,"Percolator.SVMScore":1.371,"Precursor.Area":7256132.5,"Apex.RT..min.":7.73,"ptmRS..Binomial.Peptide.Score":263.17,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(8): 877.50; y2+-H(3) O(4) P(12): 671.34; y2+-H(3) O(4) P(10): 544.32; y2+-H(3) O(4) P(8): 439.25; y1+(4): 402.23; y1+(3): 305.18","ptmRS..Best.Site.Probabilities":"S3(Phospho): 100; S9(Phospho): 99.99","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(3): 100.0; S(9): 100.0; T(11): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (X4)","Search.ID":"X","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DFLLKPELSR","Annotated.Sequence":"[K].DFLLKPELSR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.5031,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":406.56715,"Contaminant":"False","Aligned.m.z..Da.":406.56719,"MH...Da.":1217.68691,"Theo..MH...Da.":1217.68885,"DeltaM..ppm.":-1.59,"Deltam.z..Da.":-0.00065,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":767389.1875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":36.79317,"Ion.Inject.Time..ms.":22,"RT..min.":13.1427,"First.Scan":12364,"Last.Scan":12364,"Master.Scan.s.":12359,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_01.raw","File.ID":"F13","Quan.Info":"","Peptides.Matched":287,"XCorr":1.59,"X..Protein.Groups":1,"Percolator.q.Value":0.00043,"Percolator.PEP":0.00179,"Percolator.SVMScore":0.988,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (X4)","Search.ID":"X","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MGSINNNFNTNNNSNTDLDR","Annotated.Sequence":"[-].mGSINNNFNTNNNsNTDLDR.[D]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S14(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P39517","Master.Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","Protein.Accessions":"P39517","Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.1579,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":749.31202,"Contaminant":"False","Aligned.m.z..Da.":749.312,"MH...Da.":2245.92153,"Theo..MH...Da.":2245.91057,"DeltaM..ppm.":4.88,"Deltam.z..Da.":0.00365,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":478744.53125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":38.13672,"Ion.Inject.Time..ms.":22,"RT..min.":12.0152,"First.Scan":10873,"Last.Scan":10873,"Master.Scan.s.":10863,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_01.raw","File.ID":"F13","Quan.Info":"","Peptides.Matched":1474,"XCorr":1.71,"X..Protein.Groups":1,"Percolator.q.Value":0.00043,"Percolator.PEP":0.00342,"Percolator.SVMScore":0.852,"ptmRS..Binomial.Peptide.Score":146.62,"ptmRS..Isoform.Confidence.Probability":0.99,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(9): 1030.45; y1+-H(3) O(4) P(8): 916.41; y1+-H(3) O(4) P(7): 802.37; y2+-H(3) O(4) P(7): 401.69; y1+(5): 619.30","ptmRS..Best.Site.Probabilities":"S14(Phospho): 98.96","ptmRS..Phospho.Site.Probabilities":"S(3): 0.0; T(10): 0.0; S(14): 99.0; T(16): 1.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (X4)","Search.ID":"X","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"GVFGYGFEEPSAIQQR","Annotated.Sequence":"[R].GVFGYGFEEPSAIQQR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.6079,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":595.62382,"Contaminant":"False","Aligned.m.z..Da.":595.62389,"MH...Da.":1784.85692,"Theo..MH...Da.":1784.86022,"DeltaM..ppm.":-1.85,"Deltam.z..Da.":-0.0011,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":179078.32812,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":14.85865,"Ion.Inject.Time..ms.":22,"RT..min.":15.0155,"First.Scan":14409,"Last.Scan":14409,"Master.Scan.s.":14404,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_01.raw","File.ID":"F13","Quan.Info":"","Peptides.Matched":1359,"XCorr":2.27,"X..Protein.Groups":1,"Percolator.q.Value":0.00043,"Percolator.PEP":0.00076,"Percolator.SVMScore":1.171,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (X4)","Search.ID":"X","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KFQDDTLNR","Annotated.Sequence":"[K].KFQDDtLNR.[V]","Modifications":"T6(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6602,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":608.77179,"Contaminant":"False","Aligned.m.z..Da.":608.77183,"MH...Da.":1216.5363,"Theo..MH...Da.":1216.53579,"DeltaM..ppm.":0.42,"Deltam.z..Da.":0.00026,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2307778.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":12.40544,"Ion.Inject.Time..ms.":22,"RT..min.":6.1995,"First.Scan":3133,"Last.Scan":3133,"Master.Scan.s.":3128,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_01.raw","File.ID":"F13","Quan.Info":"","Peptides.Matched":620,"XCorr":2.59,"X..Protein.Groups":1,"Percolator.q.Value":0.00043,"Percolator.PEP":0.00056,"Percolator.SVMScore":1.235,"Precursor.Area":1467100.375,"Apex.RT..min.":6.2,"ptmRS..Binomial.Peptide.Score":337.92,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"T6(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"T(6): 100.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AH4)","Search.ID":"AH","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSSPLHRLSPTGK","Annotated.Sequence":"[R].mSsPLHRLsPTGK.[S]","Modifications":"M1(Oxidation); S3(Phospho); S9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.5909,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":529.56467,"Contaminant":"False","Aligned.m.z..Da.":529.56474,"MH...Da.":1586.67946,"Theo..MH...Da.":1586.67977,"DeltaM..ppm.":-0.19,"Deltam.z..Da.":-0.0001,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1183573.875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":46.40282,"Ion.Inject.Time..ms.":22,"RT..min.":7.7389,"First.Scan":5499,"Last.Scan":5499,"Master.Scan.s.":5487,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y25_03.raw","File.ID":"F3","Quan.Info":"","Peptides.Matched":1026,"XCorr":2.64,"X..Protein.Groups":1,"Percolator.q.Value":0.00043,"Percolator.PEP":0.00366,"Percolator.SVMScore":0.723,"ptmRS..Binomial.Peptide.Score":185.3,"ptmRS..Isoform.Confidence.Probability":0.5,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(8): 877.50; y2+-H(3) O(4) P(11): 627.82; y2+-H(3) O(4) P(10): 544.32; y2+-H(3) O(4) P(8): 439.25; y1+(4): 402.23","ptmRS..Best.Site.Probabilities":"S3(Phospho): 100; S9(Phospho): 50; T11(Phospho): 50","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(3): 100.0; S(9): 50.0; T(11): 50.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Y4)","Search.ID":"Y","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KSDEPSRESTPVR","Annotated.Sequence":"[K].KSDEPsREsTPVR.[S]","Modifications":"S6(Phospho); S9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q08421","Master.Protein.Descriptions":"Enhancer of translation termination 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ETT1 PE=1 SV=1","Protein.Accessions":"Q08421","Protein.Descriptions":"Enhancer of translation termination 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ETT1 PE=1 SV=1","X..Missed.Cleavages":2,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1354,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":824.34228,"Contaminant":"False","Aligned.m.z..Da.":824.34215,"MH...Da.":1647.6773,"Theo..MH...Da.":1647.67752,"DeltaM..ppm.":-0.14,"Deltam.z..Da.":-0.00011,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":7673565.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":38.04137,"Ion.Inject.Time..ms.":22,"RT..min.":4.5226,"First.Scan":1466,"Last.Scan":1466,"Master.Scan.s.":1455,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_06.raw","File.ID":"F12","Quan.Info":"","Peptides.Matched":967,"XCorr":2.29,"X..Protein.Groups":1,"Percolator.q.Value":0.00044,"Percolator.PEP":0.00241,"Percolator.SVMScore":0.925,"ptmRS..Binomial.Peptide.Score":394.77,"ptmRS..Isoform.Confidence.Probability":0.5,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(10): 1219.55; y1+-H(3) O(4) P(9): 1090.50; y2+-H(3) O(4) P(10): 610.28; y2+-H(3) O(4) P(9): 545.76; b1+- -2H(3) O(4) P -2H(3) O(4) P(9): 980.44; b1+- -2H(3) O(4) P -2H(3) O(4) P(10): 1081.49; b1+- -2H(3) O(4) P -2H(3) O(4) P(12): 1277.61","ptmRS..Best.Site.Probabilities":"S6(Phospho): 100; S9(Phospho): 50; T10(Phospho): 50","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(6): 100.0; S(9): 50.0; T(10): 50.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Q4)","Search.ID":"Q","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DIFSNDELLSDAYDAK","Annotated.Sequence":"[K].DIFSNDELLSDAYDAK.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.625,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":605.94792,"Contaminant":"False","Aligned.m.z..Da.":605.94771,"MH...Da.":1815.82922,"Theo..MH...Da.":1815.82831,"DeltaM..ppm.":0.5,"Deltam.z..Da.":0.0003,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":378353.0625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":5.36907,"Ion.Inject.Time..ms.":22,"RT..min.":17.0793,"First.Scan":17378,"Last.Scan":17378,"Master.Scan.s.":17374,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_02.raw","File.ID":"F20","Quan.Info":"","Peptides.Matched":1567,"XCorr":2.32,"X..Protein.Groups":1,"Percolator.q.Value":0.00047,"Percolator.PEP":0.00013,"Percolator.SVMScore":1.517,"Precursor.Area":475329.9375,"Apex.RT..min.":17.1,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (L4)","Search.ID":"L","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LQETNPEEVPKFEK","Annotated.Sequence":"[K].LQETNPEEVPKFEK.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.5771,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":563.2904,"Contaminant":"False","Aligned.m.z..Da.":563.29036,"MH...Da.":1687.85665,"Theo..MH...Da.":1687.85374,"DeltaM..ppm.":1.72,"Deltam.z..Da.":0.00097,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2498656.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":47.44489,"Ion.Inject.Time..ms.":22,"RT..min.":9.3135,"First.Scan":7430,"Last.Scan":7430,"Master.Scan.s.":7419,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_01.raw","File.ID":"F25","Quan.Info":"","Peptides.Matched":870,"XCorr":1.75,"X..Protein.Groups":1,"Percolator.q.Value":0.00047,"Percolator.PEP":6.11e-07,"Percolator.SVMScore":2.561,"Precursor.Area":1855358.75,"Apex.RT..min.":9.31,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (L4)","Search.ID":"L","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"APQALMLAPTR","Annotated.Sequence":"[K].APQALMLAPTR.[E]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6154,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":584.82924,"Contaminant":"False","Aligned.m.z..Da.":584.82924,"MH...Da.":1168.65121,"Theo..MH...Da.":1168.65069,"DeltaM..ppm.":0.45,"Deltam.z..Da.":0.00026,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1081589.125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":75.80492,"Ion.Inject.Time..ms.":22,"RT..min.":11.6217,"First.Scan":10274,"Last.Scan":10274,"Master.Scan.s.":10266,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_01.raw","File.ID":"F25","Quan.Info":"","Peptides.Matched":162,"XCorr":2.99,"X..Protein.Groups":1,"Percolator.q.Value":0.00047,"Percolator.PEP":1.045e-06,"Percolator.SVMScore":2.456,"Precursor.Area":904758.1875,"Apex.RT..min.":11.63,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (L4)","Search.ID":"L","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"TGTFSIAALQR","Annotated.Sequence":"[K].TGTFSIAALQR.[I]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7582,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":582.82266,"Contaminant":"False","Aligned.m.z..Da.":582.82279,"MH...Da.":1164.63804,"Theo..MH...Da.":1164.63715,"DeltaM..ppm.":0.77,"Deltam.z..Da.":0.00045,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":697429.625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":20.50095,"Ion.Inject.Time..ms.":22,"RT..min.":13.3521,"First.Scan":12625,"Last.Scan":12625,"Master.Scan.s.":12619,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_01.raw","File.ID":"F25","Quan.Info":"","Peptides.Matched":360,"XCorr":3.35,"X..Protein.Groups":1,"Percolator.q.Value":0.00047,"Percolator.PEP":9.451e-07,"Percolator.SVMScore":2.475,"Precursor.Area":2161446.75,"Apex.RT..min.":13.37,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (L4)","Search.ID":"L","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"IQDPQMTGYVSTR","Annotated.Sequence":"[R].IQDPQmTGyVSTR.[Y]","Modifications":"M6(Oxidation); Y9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P32485","Master.Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","Protein.Accessions":"P32485","Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.0798,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":796.34653,"Contaminant":"False","Aligned.m.z..Da.":796.34662,"MH...Da.":1591.68578,"Theo..MH...Da.":1591.6822,"DeltaM..ppm.":2.25,"Deltam.z..Da.":0.00179,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1443452,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":38.20189,"Ion.Inject.Time..ms.":22,"RT..min.":7.7371,"First.Scan":5561,"Last.Scan":5561,"Master.Scan.s.":5560,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_01.raw","File.ID":"F25","Quan.Info":"","Peptides.Matched":1132,"XCorr":2.63,"X..Protein.Groups":1,"Percolator.q.Value":0.00047,"Percolator.PEP":5.123e-06,"Percolator.SVMScore":2.145,"Precursor.Area":6478991.5,"Apex.RT..min.":7.7,"ptmRS..Binomial.Peptide.Score":241.49,"ptmRS..Isoform.Confidence.Probability":0.5,"ptmRS..Isoform.Group.Report":"b1+(7): 910.34","ptmRS..Best.Site.Probabilities":"T7(Phospho): 50; Y9(Phospho): 50","ptmRS..Phospho.Site.Probabilities":"T(7): 50.0; Y(9): 50.0; S(11): 0.0; T(12): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (L4)","Search.ID":"L","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].msEGITDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S2(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1329,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1096.46631,"Contaminant":"False","Aligned.m.z..Da.":1096.46615,"MH...Da.":2191.92536,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":-1.13,"Deltam.z..Da.":-0.00124,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":310190.96875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":51.62367,"Ion.Inject.Time..ms.":22,"RT..min.":16.0871,"First.Scan":16008,"Last.Scan":16008,"Master.Scan.s.":16006,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_01.raw","File.ID":"F25","Quan.Info":"","Peptides.Matched":1869,"XCorr":2.86,"X..Protein.Groups":1,"Percolator.q.Value":0.00047,"Percolator.PEP":6.78e-06,"Percolator.SVMScore":2.09,"Precursor.Area":16847080,"Apex.RT..min.":15.79,"ptmRS..Binomial.Peptide.Score":397.68,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(2): 112.04; b1+-H(3) O(4) P(3): 241.08; b1+-H(3) O(4) P(4): 298.10; b1+-H(3) O(4) P(5): 411.19; b1+-H(3) O(4) P(6): 512.24; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+-H(3) O(4) P(9): 869.39; y1+(14): 1683.77","ptmRS..Best.Site.Probabilities":"S2(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 100.0; T(6): 0.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (L4)","Search.ID":"L","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FDDMELDENLLR","Annotated.Sequence":"[K].FDDmELDENLLR.[G]","Modifications":"M4(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.4609,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":763.34482,"Contaminant":"False","Aligned.m.z..Da.":763.34497,"MH...Da.":1525.68236,"Theo..MH...Da.":1525.6839,"DeltaM..ppm.":-1.01,"Deltam.z..Da.":-0.00077,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":609448.375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":90.05635,"Ion.Inject.Time..ms.":22,"RT..min.":14.4647,"First.Scan":14037,"Last.Scan":14037,"Master.Scan.s.":14026,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_01.raw","File.ID":"F25","Quan.Info":"","Peptides.Matched":1042,"XCorr":2.3,"X..Protein.Groups":1,"Percolator.q.Value":0.00047,"Percolator.PEP":3.012e-06,"Percolator.SVMScore":2.249,"Precursor.Area":1498670.5,"Apex.RT..min.":14.48,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (L4)","Search.ID":"L","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"AGNFALHPEVVR","Annotated.Sequence":"[R].AGNFALHPEVVR.[E]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q03558","Master.Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","Protein.Accessions":"Q03558","Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.7356,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":437.23828,"Contaminant":"False","Aligned.m.z..Da.":437.23822,"MH...Da.":1309.7003,"Theo..MH...Da.":1309.70114,"DeltaM..ppm.":-0.64,"Deltam.z..Da.":-0.00028,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2175168.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":9.3614,"Ion.Inject.Time..ms.":22,"RT..min.":10.0393,"First.Scan":8271,"Last.Scan":8271,"Master.Scan.s.":8264,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_01.raw","File.ID":"F25","Quan.Info":"","Peptides.Matched":392,"XCorr":2.95,"X..Protein.Groups":1,"Percolator.q.Value":0.00047,"Percolator.PEP":9.592e-07,"Percolator.SVMScore":2.472,"Precursor.Area":1144696,"Apex.RT..min.":10.04,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (L4)","Search.ID":"L","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LQETNPEEVPK","Annotated.Sequence":"[K].LQETNPEEVPK.[F]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5347,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":642.32819,"Contaminant":"False","Aligned.m.z..Da.":642.32825,"MH...Da.":1283.6491,"Theo..MH...Da.":1283.64777,"DeltaM..ppm.":1.04,"Deltam.z..Da.":0.00066,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3955879,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":4.67424,"Ion.Inject.Time..ms.":22,"RT..min.":7.65,"First.Scan":5434,"Last.Scan":5434,"Master.Scan.s.":5430,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_01.raw","File.ID":"F25","Quan.Info":"","Peptides.Matched":521,"XCorr":2.88,"X..Protein.Groups":1,"Percolator.q.Value":0.00048,"Percolator.PEP":0.00001,"Percolator.SVMScore":1.988,"Precursor.Area":9008494,"Apex.RT..min.":7.67,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (L4)","Search.ID":"L","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KFQDDTLNR","Annotated.Sequence":"[K].KFQDDtLNR.[V]","Modifications":"T6(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6578,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":608.77176,"Contaminant":"False","Aligned.m.z..Da.":608.77177,"MH...Da.":1216.53625,"Theo..MH...Da.":1216.53579,"DeltaM..ppm.":0.37,"Deltam.z..Da.":0.00023,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2760365.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":10.21892,"Ion.Inject.Time..ms.":22,"RT..min.":6.22,"First.Scan":3112,"Last.Scan":3112,"Master.Scan.s.":3103,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_01.raw","File.ID":"F25","Quan.Info":"","Peptides.Matched":622,"XCorr":3.01,"X..Protein.Groups":1,"Percolator.q.Value":0.00048,"Percolator.PEP":0.00002,"Percolator.SVMScore":1.851,"Precursor.Area":2583344.25,"Apex.RT..min.":6.23,"ptmRS..Binomial.Peptide.Score":380.32,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"T6(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"T(6): 100.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (L4)","Search.ID":"L","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"SSQIHIENESTEDILK","Annotated.Sequence":"[R].SSQIHIENEStEDILK.[I]","Modifications":"T11(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0189,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":641.62948,"Contaminant":"False","Aligned.m.z..Da.":641.62947,"MH...Da.":1922.8739,"Theo..MH...Da.":1922.87429,"DeltaM..ppm.":-0.2,"Deltam.z..Da.":-0.00013,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":898083.5625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":27.15373,"Ion.Inject.Time..ms.":22,"RT..min.":11.2874,"First.Scan":9821,"Last.Scan":9821,"Master.Scan.s.":9811,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_01.raw","File.ID":"F25","Quan.Info":"","Peptides.Matched":1805,"XCorr":3.18,"X..Protein.Groups":1,"Percolator.q.Value":0.00048,"Percolator.PEP":0.00002,"Percolator.SVMScore":1.856,"Precursor.Area":616042.0625,"Apex.RT..min.":11.29,"ptmRS..Binomial.Peptide.Score":329.27,"ptmRS..Isoform.Confidence.Probability":0.996,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(7): 787.42; y1+-H(3) O(4) P(6): 700.39; y2+-H(3) O(4) P(7): 394.21","ptmRS..Best.Site.Probabilities":"T11(Phospho): 99.56","ptmRS..Phospho.Site.Probabilities":"S(1): 0.0; S(2): 0.0; S(10): 0.4; T(11): 99.6"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (L4)","Search.ID":"L","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"TAVFYGGTPISK","Annotated.Sequence":"[K].TAVFYGGTPISK.[D]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.4599,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":620.8321,"Contaminant":"False","Aligned.m.z..Da.":620.83209,"MH...Da.":1240.65692,"Theo..MH...Da.":1240.65721,"DeltaM..ppm.":-0.24,"Deltam.z..Da.":-0.00015,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1399320,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":52.91119,"Ion.Inject.Time..ms.":22,"RT..min.":11.139,"First.Scan":9624,"Last.Scan":9624,"Master.Scan.s.":9616,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_01.raw","File.ID":"F25","Quan.Info":"","Peptides.Matched":386,"XCorr":2.37,"X..Protein.Groups":1,"Percolator.q.Value":0.00048,"Percolator.PEP":0.00025,"Percolator.SVMScore":1.387,"Precursor.Area":813453.5625,"Apex.RT..min.":11.13,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (L4)","Search.ID":"L","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"TAVFYGGTPISK","Annotated.Sequence":"[K].TAVFYGGTPISK.[D]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5301,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":620.8321,"Contaminant":"False","Aligned.m.z..Da.":620.83209,"MH...Da.":1240.65693,"Theo..MH...Da.":1240.65721,"DeltaM..ppm.":-0.23,"Deltam.z..Da.":-0.00014,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1399320,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":54.8198,"Ion.Inject.Time..ms.":22,"RT..min.":11.1365,"First.Scan":9620,"Last.Scan":9620,"Master.Scan.s.":9616,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_01.raw","File.ID":"F25","Quan.Info":"","Peptides.Matched":386,"XCorr":2.49,"X..Protein.Groups":1,"Percolator.q.Value":0.00048,"Percolator.PEP":0.00076,"Percolator.SVMScore":1.169,"Precursor.Area":813453.5625,"Apex.RT..min.":11.13,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (L4)","Search.ID":"L","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSSPLHRLSPTGK","Annotated.Sequence":"[R].mSsPLHRLsPTGK.[S]","Modifications":"M1(Oxidation); S3(Phospho); S9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.4609,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":529.56463,"Contaminant":"False","Aligned.m.z..Da.":529.56468,"MH...Da.":1586.67933,"Theo..MH...Da.":1586.67977,"DeltaM..ppm.":-0.28,"Deltam.z..Da.":-0.00015,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3016031.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":3.95795,"Ion.Inject.Time..ms.":22,"RT..min.":7.7227,"First.Scan":5542,"Last.Scan":5542,"Master.Scan.s.":5534,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_01.raw","File.ID":"F25","Quan.Info":"","Peptides.Matched":1007,"XCorr":3.71,"X..Protein.Groups":1,"Percolator.q.Value":0.00048,"Percolator.PEP":0.00007,"Percolator.SVMScore":1.638,"Precursor.Area":6916300,"Apex.RT..min.":7.74,"ptmRS..Binomial.Peptide.Score":298.61,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(8): 877.50; y2+-H(3) O(4) P(12): 671.34; y2+-H(3) O(4) P(11): 627.82; y2+-H(3) O(4) P(10): 544.32; y2+-H(3) O(4) P(9): 495.80; y2+-H(3) O(4) P(8): 439.25; y1+(4): 402.23; y1+(3): 305.18","ptmRS..Best.Site.Probabilities":"S3(Phospho): 100; S9(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(3): 100.0; S(9): 100.0; T(11): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (L4)","Search.ID":"L","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSSPLHRLSPTGK","Annotated.Sequence":"[R].mSsPLHRLsPTGK.[S]","Modifications":"M1(Oxidation); S3(Phospho); S9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5209,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":793.84373,"Contaminant":"False","Aligned.m.z..Da.":793.84382,"MH...Da.":1586.68018,"Theo..MH...Da.":1586.67977,"DeltaM..ppm.":0.26,"Deltam.z..Da.":0.0002,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3154458,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":11.51202,"Ion.Inject.Time..ms.":22,"RT..min.":7.7522,"First.Scan":5585,"Last.Scan":5585,"Master.Scan.s.":5573,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_01.raw","File.ID":"F25","Quan.Info":"","Peptides.Matched":983,"XCorr":3.11,"X..Protein.Groups":1,"Percolator.q.Value":0.00048,"Percolator.PEP":0.00002,"Percolator.SVMScore":1.887,"Precursor.Area":2405892.75,"Apex.RT..min.":7.75,"ptmRS..Binomial.Peptide.Score":360.01,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(10): 1087.64; y1+-H(3) O(4) P(8): 877.50; y1+-H(3) O(4) P(6): 584.34; y1+-H(3) O(4) P(5): 471.26; y2+-H(3) O(4) P(11): 627.82; y2+-H(3) O(4) P(10): 544.32; b1+- -2H(3) O(4) P -2H(3) O(4) P(9): 989.50; y1+(4): 402.23; y1+(3): 305.18","ptmRS..Best.Site.Probabilities":"S3(Phospho): 100; S9(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(3): 100.0; S(9): 100.0; T(11): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (L4)","Search.ID":"L","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"IQDPQMTGYVSTR","Annotated.Sequence":"[R].IQDPQmtGYVsTR.[Y]","Modifications":"M6(Oxidation); T7(Phospho); S11(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P32485","Master.Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","Protein.Accessions":"P32485","Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.0475,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":836.32835,"Contaminant":"False","Aligned.m.z..Da.":836.32845,"MH...Da.":1671.64942,"Theo..MH...Da.":1671.64853,"DeltaM..ppm.":0.53,"Deltam.z..Da.":0.00045,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3314364,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":17.05342,"Ion.Inject.Time..ms.":22,"RT..min.":7.7517,"First.Scan":5584,"Last.Scan":5584,"Master.Scan.s.":5573,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_01.raw","File.ID":"F25","Quan.Info":"","Peptides.Matched":755,"XCorr":2.95,"X..Protein.Groups":1,"Percolator.q.Value":0.00048,"Percolator.PEP":0.00005,"Percolator.SVMScore":1.708,"Precursor.Area":2932849.25,"Apex.RT..min.":7.75,"ptmRS..Binomial.Peptide.Score":357.84,"ptmRS..Isoform.Confidence.Probability":0.005,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(11): 1332.53; y1+-H(3) O(4) P(10): 1217.50; y1+-H(3) O(4) P(8): 992.39; y1+-H(3) O(4) P(7): 845.36; y2+-H(3) O(4) P(11): 666.77; y2+-H(3) O(4) P(10): 609.25; y2+-H(3) O(4) P(8): 496.70; y1+(4): 462.27; y1+(3): 363.20","ptmRS..Best.Site.Probabilities":"T7(Phospho): 100; Y9(Phospho): 99.08","ptmRS..Phospho.Site.Probabilities":"T(7): 100.0; Y(9): 99.1; S(11): 0.5; T(12): 0.5"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (L4)","Search.ID":"L","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"IGNNELLHR","Annotated.Sequence":"[K].IGNNELLHR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q03558","Master.Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","Protein.Accessions":"Q03558","Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.5714,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":355.86497,"Contaminant":"False","Aligned.m.z..Da.":355.865,"MH...Da.":1065.58036,"Theo..MH...Da.":1065.57997,"DeltaM..ppm.":0.36,"Deltam.z..Da.":0.00013,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2242477.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":9.57172,"Ion.Inject.Time..ms.":22,"RT..min.":7.5908,"First.Scan":5346,"Last.Scan":5346,"Master.Scan.s.":5339,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_01.raw","File.ID":"F25","Quan.Info":"","Peptides.Matched":300,"XCorr":2.59,"X..Protein.Groups":1,"Percolator.q.Value":0.00048,"Percolator.PEP":7.731e-06,"Percolator.SVMScore":2.065,"Precursor.Area":1734542.75,"Apex.RT..min.":7.59,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (L4)","Search.ID":"L","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LEAGLSDSK","Annotated.Sequence":"[K].LEAGLsDSK.[Q]","Modifications":"S6(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.0692,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":500.22389,"Contaminant":"False","Aligned.m.z..Da.":500.22393,"MH...Da.":999.4405,"Theo..MH...Da.":999.43943,"DeltaM..ppm.":1.07,"Deltam.z..Da.":0.00054,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2343035.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":26.00399,"Ion.Inject.Time..ms.":22,"RT..min.":7.5903,"First.Scan":5345,"Last.Scan":5345,"Master.Scan.s.":5339,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_01.raw","File.ID":"F25","Quan.Info":"","Peptides.Matched":749,"XCorr":2.6,"X..Protein.Groups":1,"Percolator.q.Value":0.00048,"Percolator.PEP":0.00007,"Percolator.SVMScore":1.644,"Precursor.Area":3358874.25,"Apex.RT..min.":7.6,"ptmRS..Binomial.Peptide.Score":250.57,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(8): 788.38; y1+-H(3) O(4) P(7): 659.34; y1+-H(3) O(4) P(6): 588.30; y1+-H(3) O(4) P(5): 531.28; y1+-H(3) O(4) P(4): 418.19; y1+(3): 349.17; y1+(2): 234.14","ptmRS..Best.Site.Probabilities":"S6(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(6): 100.0; S(8): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (L4)","Search.ID":"L","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"IQDPQMTGYVSTR","Annotated.Sequence":"[R].IQDPQMtGYVsTR.[Y]","Modifications":"T7(Phospho); S11(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P32485","Master.Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","Protein.Accessions":"P32485","Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.0426,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":828.32954,"Contaminant":"False","Aligned.m.z..Da.":828.32941,"MH...Da.":1655.6518,"Theo..MH...Da.":1655.65362,"DeltaM..ppm.":-1.09,"Deltam.z..Da.":-0.00091,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2434621,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":66.80309,"Ion.Inject.Time..ms.":22,"RT..min.":9.9094,"First.Scan":8118,"Last.Scan":8118,"Master.Scan.s.":8108,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_01.raw","File.ID":"F25","Quan.Info":"","Peptides.Matched":760,"XCorr":1.88,"X..Protein.Groups":1,"Percolator.q.Value":0.00048,"Percolator.PEP":0.00019,"Percolator.SVMScore":1.442,"Precursor.Area":1723377.75,"Apex.RT..min.":9.91,"ptmRS..Binomial.Peptide.Score":267.35,"ptmRS..Isoform.Confidence.Probability":0.003,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(11): 1316.53; y1+-H(3) O(4) P(10): 1201.51; y1+-H(3) O(4) P(8): 976.40; y1+-H(3) O(4) P(7): 845.36; y2+-H(3) O(4) P(10): 601.26; y1+(4): 462.27; y1+(3): 363.20","ptmRS..Best.Site.Probabilities":"T7(Phospho): 99.7; Y9(Phospho): 49.7; T12(Phospho): 50","ptmRS..Phospho.Site.Probabilities":"T(7): 99.7; Y(9): 49.7; S(11): 0.6; T(12): 50.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (L4)","Search.ID":"L","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"NHQLEEEIEK","Annotated.Sequence":"[K].NHQLEEEIEK.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.3755,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":634.81008,"Contaminant":"False","Aligned.m.z..Da.":634.81008,"MH...Da.":1268.61289,"Theo..MH...Da.":1268.61172,"DeltaM..ppm.":0.92,"Deltam.z..Da.":0.00059,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":904011.1875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":12.10993,"Ion.Inject.Time..ms.":22,"RT..min.":7.141,"First.Scan":4610,"Last.Scan":4610,"Master.Scan.s.":4598,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_01.raw","File.ID":"F25","Quan.Info":"","Peptides.Matched":873,"XCorr":2.29,"X..Protein.Groups":1,"Percolator.q.Value":0.00048,"Percolator.PEP":0.00004,"Percolator.SVMScore":1.77,"Precursor.Area":624333.9375,"Apex.RT..min.":7.13,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (L4)","Search.ID":"L","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LEAGLSDSK","Annotated.Sequence":"[K].LEAGLSDSK.[Q]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.3915,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":460.24023,"Contaminant":"False","Aligned.m.z..Da.":460.24022,"MH...Da.":919.47319,"Theo..MH...Da.":919.4731,"DeltaM..ppm.":0.1,"Deltam.z..Da.":0.00004,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1967285.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":21.55582,"Ion.Inject.Time..ms.":22,"RT..min.":6.5138,"First.Scan":3546,"Last.Scan":3546,"Master.Scan.s.":3545,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_01.raw","File.ID":"F25","Quan.Info":"","Peptides.Matched":412,"XCorr":2.12,"X..Protein.Groups":1,"Percolator.q.Value":0.00048,"Percolator.PEP":0.00016,"Percolator.SVMScore":1.47,"Precursor.Area":1122943.625,"Apex.RT..min.":6.51,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (L4)","Search.ID":"L","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KDELTLEGIK","Annotated.Sequence":"[K].KDELTLEGIK.[Q]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.5786,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":382.55164,"Contaminant":"False","Aligned.m.z..Da.":382.55159,"MH...Da.":1145.64038,"Theo..MH...Da.":1145.64123,"DeltaM..ppm.":-0.75,"Deltam.z..Da.":-0.00028,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3243620,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":2.89846,"Ion.Inject.Time..ms.":22,"RT..min.":10.2433,"First.Scan":8513,"Last.Scan":8513,"Master.Scan.s.":8511,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_01.raw","File.ID":"F25","Quan.Info":"","Peptides.Matched":339,"XCorr":3.37,"X..Protein.Groups":1,"Percolator.q.Value":0.00048,"Percolator.PEP":0.00003,"Percolator.SVMScore":1.832,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (L4)","Search.ID":"L","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DFLLKPELSR","Annotated.Sequence":"[K].DFLLKPELSR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.5289,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":406.56762,"Contaminant":"False","Aligned.m.z..Da.":406.5677,"MH...Da.":1217.68832,"Theo..MH...Da.":1217.68885,"DeltaM..ppm.":-0.44,"Deltam.z..Da.":-0.00018,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1016786.125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":19.3837,"Ion.Inject.Time..ms.":22,"RT..min.":13.1483,"First.Scan":12363,"Last.Scan":12363,"Master.Scan.s.":12359,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_01.raw","File.ID":"F25","Quan.Info":"","Peptides.Matched":276,"XCorr":2.25,"X..Protein.Groups":1,"Percolator.q.Value":0.00048,"Percolator.PEP":0.00356,"Percolator.SVMScore":0.866,"Precursor.Area":654890.5,"Apex.RT..min.":13.15,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (L4)","Search.ID":"L","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KSDEPSRESTPVR","Annotated.Sequence":"[K].KSDEPsREsTPVR.[S]","Modifications":"S6(Phospho); S9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q08421","Master.Protein.Descriptions":"Enhancer of translation termination 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ETT1 PE=1 SV=1","Protein.Accessions":"Q08421","Protein.Descriptions":"Enhancer of translation termination 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ETT1 PE=1 SV=1","X..Missed.Cleavages":2,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1179,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":824.34211,"Contaminant":"False","Aligned.m.z..Da.":824.34197,"MH...Da.":1647.67694,"Theo..MH...Da.":1647.67752,"DeltaM..ppm.":-0.35,"Deltam.z..Da.":-0.00029,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":17717376,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":1.39547,"Ion.Inject.Time..ms.":8.687,"RT..min.":4.5543,"First.Scan":1329,"Last.Scan":1329,"Master.Scan.s.":1322,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_01.raw","File.ID":"F25","Quan.Info":"","Peptides.Matched":970,"XCorr":2.63,"X..Protein.Groups":1,"Percolator.q.Value":0.00048,"Percolator.PEP":0.00033,"Percolator.SVMScore":1.331,"Precursor.Area":7806447.5,"Apex.RT..min.":4.55,"ptmRS..Binomial.Peptide.Score":373.33,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(9): 998.45; y1+-H(3) O(4) P(10): 1219.55; y1+-H(3) O(4) P(9): 1090.50; y1+-H(3) O(4) P(4): 454.28; y2+-H(3) O(4) P(10): 610.28; y2+-H(3) O(4) P(9): 545.76; b1+- -2H(3) O(4) P -2H(3) O(4) P(10): 1081.49; b1+- -2H(3) O(4) P -2H(3) O(4) P(12): 1277.61","ptmRS..Best.Site.Probabilities":"S6(Phospho): 100; T10(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(6): 100.0; S(9): 0.0; T(10): 100.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (L4)","Search.ID":"L","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LQQTAFDKK","Annotated.Sequence":"[R].LQQTAFDKK.[S]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.4449,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":360.20109,"Contaminant":"False","Aligned.m.z..Da.":360.20112,"MH...Da.":1078.58871,"Theo..MH...Da.":1078.58914,"DeltaM..ppm.":-0.39,"Deltam.z..Da.":-0.00014,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":4786174,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":39.58683,"Ion.Inject.Time..ms.":14.274,"RT..min.":5.4803,"First.Scan":2224,"Last.Scan":2224,"Master.Scan.s.":2219,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_01.raw","File.ID":"F25","Quan.Info":"","Peptides.Matched":392,"XCorr":2.54,"X..Protein.Groups":1,"Percolator.q.Value":0.00048,"Percolator.PEP":0.00154,"Percolator.SVMScore":1.031,"Precursor.Area":2771812.75,"Apex.RT..min.":5.48,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (L4)","Search.ID":"L","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LQQTAFDKK","Annotated.Sequence":"[R].LQQTAFDKK.[S]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":4,"DeltaScore":0.4035,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":360.20118,"Contaminant":"False","Aligned.m.z..Da.":360.20121,"MH...Da.":1078.58899,"Theo..MH...Da.":1078.58914,"DeltaM..ppm.":-0.14,"Deltam.z..Da.":-0.00005,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2506400,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":61.07931,"Ion.Inject.Time..ms.":20.773,"RT..min.":5.4733,"First.Scan":2218,"Last.Scan":2218,"Master.Scan.s.":2206,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_01.raw","File.ID":"F25","Quan.Info":"","Peptides.Matched":392,"XCorr":2.85,"X..Protein.Groups":1,"Percolator.q.Value":0.00048,"Percolator.PEP":0.00022,"Percolator.SVMScore":1.413,"Precursor.Area":2771812.75,"Apex.RT..min.":5.48,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (L4)","Search.ID":"L","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"EDGTTPFVAIWK","Annotated.Sequence":"[R].EDGTTPFVAIWK.[H]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6368,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":682.34693,"Contaminant":"False","Aligned.m.z..Da.":682.34685,"MH...Da.":1363.68659,"Theo..MH...Da.":1363.68924,"DeltaM..ppm.":-1.95,"Deltam.z..Da.":-0.00133,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":360591.59375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":16.899,"First.Scan":17029,"Last.Scan":17029,"Master.Scan.s.":17026,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_01.raw","File.ID":"F25","Quan.Info":"","Peptides.Matched":665,"XCorr":1.9,"X..Protein.Groups":1,"Percolator.q.Value":0.00048,"Percolator.PEP":0.00329,"Percolator.SVMScore":0.882,"Precursor.Area":231160.8125,"Apex.RT..min.":16.9,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (L4)","Search.ID":"L","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DIFSNDELLSDAYDAK","Annotated.Sequence":"[K].DIFSNDELLSDAYDAK.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.6805,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":605.94814,"Contaminant":"False","Aligned.m.z..Da.":605.9481,"MH...Da.":1815.82989,"Theo..MH...Da.":1815.82831,"DeltaM..ppm.":0.87,"Deltam.z..Da.":0.00052,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":644133.125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":20.4657,"Ion.Inject.Time..ms.":22,"RT..min.":17.0624,"First.Scan":17252,"Last.Scan":17252,"Master.Scan.s.":17247,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_01.raw","File.ID":"F25","Quan.Info":"","Peptides.Matched":1618,"XCorr":2.66,"X..Protein.Groups":1,"Percolator.q.Value":0.00048,"Percolator.PEP":0.00007,"Percolator.SVMScore":1.644,"Precursor.Area":654619.625,"Apex.RT..min.":17.07,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (L4)","Search.ID":"L","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ILISTDLLAR","Annotated.Sequence":"[R].ILISTDLLAR.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5075,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":557.84541,"Contaminant":"False","Aligned.m.z..Da.":557.84536,"MH...Da.":1114.68354,"Theo..MH...Da.":1114.68304,"DeltaM..ppm.":0.45,"Deltam.z..Da.":0.00025,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1481841,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":18.75765,"Ion.Inject.Time..ms.":22,"RT..min.":15.5894,"First.Scan":15403,"Last.Scan":15403,"Master.Scan.s.":15395,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_01.raw","File.ID":"F25","Quan.Info":"","Peptides.Matched":124,"XCorr":2.68,"X..Protein.Groups":1,"Percolator.q.Value":0.00048,"Percolator.PEP":0.00003,"Percolator.SVMScore":1.812,"Precursor.Area":2190458.5,"Apex.RT..min.":15.6,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (L4)","Search.ID":"L","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MGSINNNFNTNNNSNTDLDR","Annotated.Sequence":"[-].mGSINNNFNTNNNsNTDLDR.[D]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S14(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P39517","Master.Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","Protein.Accessions":"P39517","Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.1174,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":749.31033,"Contaminant":"False","Aligned.m.z..Da.":749.31033,"MH...Da.":2245.91645,"Theo..MH...Da.":2245.91057,"DeltaM..ppm.":2.62,"Deltam.z..Da.":0.00196,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1097965,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":47.08003,"Ion.Inject.Time..ms.":22,"RT..min.":11.9944,"First.Scan":10792,"Last.Scan":10792,"Master.Scan.s.":10786,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_01.raw","File.ID":"F25","Quan.Info":"","Peptides.Matched":1459,"XCorr":2.47,"X..Protein.Groups":1,"Percolator.q.Value":0.00048,"Percolator.PEP":0.00007,"Percolator.SVMScore":1.623,"Precursor.Area":643188.625,"Apex.RT..min.":12,"ptmRS..Binomial.Peptide.Score":250.99,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(12): 1359.59; y1+-H(3) O(4) P(11): 1245.55; y1+-H(3) O(4) P(10): 1144.50; y1+-H(3) O(4) P(9): 1030.45; y1+-H(3) O(4) P(8): 916.41; y1+-H(3) O(4) P(7): 802.37; b2+-H(3) O(4) P(19): 987.41; y1+(6): 733.35; y1+(5): 619.30","ptmRS..Best.Site.Probabilities":"S14(Phospho): 99.99","ptmRS..Phospho.Site.Probabilities":"S(3): 0.0; T(10): 0.0; S(14): 100.0; T(16): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (M4)","Search.ID":"M","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DIFSNDELLSDAYDAK","Annotated.Sequence":"[K].DIFSNDELLSDAYDAK.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.704,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":605.94838,"Contaminant":"False","Aligned.m.z..Da.":605.94818,"MH...Da.":1815.8306,"Theo..MH...Da.":1815.82831,"DeltaM..ppm.":1.26,"Deltam.z..Da.":0.00076,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":580653.875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":49.64573,"Ion.Inject.Time..ms.":22,"RT..min.":17.0809,"First.Scan":17689,"Last.Scan":17689,"Master.Scan.s.":17683,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_06.raw","File.ID":"F24","Quan.Info":"","Peptides.Matched":1690,"XCorr":2.77,"X..Protein.Groups":1,"Percolator.q.Value":0.00048,"Percolator.PEP":0.00009,"Percolator.SVMScore":1.477,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (M4)","Search.ID":"M","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"TAVFYGGTPISK","Annotated.Sequence":"[K].TAVFYGGTPISK.[D]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.4864,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":620.83228,"Contaminant":"False","Aligned.m.z..Da.":620.83219,"MH...Da.":1240.65729,"Theo..MH...Da.":1240.65721,"DeltaM..ppm.":0.06,"Deltam.z..Da.":0.00004,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":651777.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":60.45061,"Ion.Inject.Time..ms.":22,"RT..min.":11.181,"First.Scan":10525,"Last.Scan":10525,"Master.Scan.s.":10523,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_06.raw","File.ID":"F24","Quan.Info":"","Peptides.Matched":385,"XCorr":2.2,"X..Protein.Groups":1,"Percolator.q.Value":0.00048,"Percolator.PEP":0.00007,"Percolator.SVMScore":1.537,"Precursor.Area":404834.6875,"Apex.RT..min.":11.18,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (M4)","Search.ID":"M","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"TAVFYGGTPISK","Annotated.Sequence":"[K].TAVFYGGTPISK.[D]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5145,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":620.83228,"Contaminant":"False","Aligned.m.z..Da.":620.83219,"MH...Da.":1240.65729,"Theo..MH...Da.":1240.65721,"DeltaM..ppm.":0.06,"Deltam.z..Da.":0.00004,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":651777.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":54.74826,"Ion.Inject.Time..ms.":22,"RT..min.":11.1867,"First.Scan":10535,"Last.Scan":10535,"Master.Scan.s.":10523,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_06.raw","File.ID":"F24","Quan.Info":"","Peptides.Matched":385,"XCorr":2.41,"X..Protein.Groups":1,"Percolator.q.Value":0.00048,"Percolator.PEP":0.0001,"Percolator.SVMScore":1.471,"Precursor.Area":404834.6875,"Apex.RT..min.":11.18,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (M4)","Search.ID":"M","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"SSQIHIENESTEDILK","Annotated.Sequence":"[R].SSQIHIENEsTEDILK.[I]","Modifications":"S10(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0072,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":641.6285,"Contaminant":"False","Aligned.m.z..Da.":641.6284,"MH...Da.":1922.87097,"Theo..MH...Da.":1922.87429,"DeltaM..ppm.":-1.73,"Deltam.z..Da.":-0.00111,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":579273.3125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":11.2649,"First.Scan":10642,"Last.Scan":10642,"Master.Scan.s.":10640,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_06.raw","File.ID":"F24","Quan.Info":"","Peptides.Matched":1787,"XCorr":2.76,"X..Protein.Groups":1,"Percolator.q.Value":0.00048,"Percolator.PEP":0.00021,"Percolator.SVMScore":1.322,"Precursor.Area":680530.5625,"Apex.RT..min.":11.25,"ptmRS..Binomial.Peptide.Score":236.62,"ptmRS..Isoform.Confidence.Probability":0.008,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(7): 787.42; y1+-H(3) O(4) P(6): 700.39; y2+-H(3) O(4) P(7): 394.21","ptmRS..Best.Site.Probabilities":"T11(Phospho): 99.25","ptmRS..Phospho.Site.Probabilities":"S(1): 0.0; S(2): 0.0; S(10): 0.8; T(11): 99.3"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (M4)","Search.ID":"M","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"KSDEPSRESTPVR","Annotated.Sequence":"[K].KSDEPsREsTPVR.[S]","Modifications":"S6(Phospho); S9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q08421","Master.Protein.Descriptions":"Enhancer of translation termination 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ETT1 PE=1 SV=1","Protein.Accessions":"Q08421","Protein.Descriptions":"Enhancer of translation termination 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ETT1 PE=1 SV=1","X..Missed.Cleavages":2,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.0451,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":824.34232,"Contaminant":"False","Aligned.m.z..Da.":824.3421,"MH...Da.":1647.67736,"Theo..MH...Da.":1647.67752,"DeltaM..ppm.":-0.1,"Deltam.z..Da.":-0.00008,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":8843401,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":19.79157,"Ion.Inject.Time..ms.":22,"RT..min.":4.4704,"First.Scan":1430,"Last.Scan":1430,"Master.Scan.s.":1423,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_06.raw","File.ID":"F24","Quan.Info":"","Peptides.Matched":967,"XCorr":2.88,"X..Protein.Groups":1,"Percolator.q.Value":0.00048,"Percolator.PEP":0.00009,"Percolator.SVMScore":1.481,"Precursor.Area":7556650.5,"Apex.RT..min.":4.48,"ptmRS..Binomial.Peptide.Score":372.85,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(9): 998.45; y1+-H(3) O(4) P(10): 1219.55; y1+-H(3) O(4) P(9): 1090.50; y1+-H(3) O(4) P(4): 454.28; y2+-H(3) O(4) P(10): 610.28; y2+-H(3) O(4) P(9): 545.76; b1+- -2H(3) O(4) P -2H(3) O(4) P(10): 1081.49; b1+- -2H(3) O(4) P -2H(3) O(4) P(12): 1277.61","ptmRS..Best.Site.Probabilities":"S6(Phospho): 100; T10(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(6): 100.0; S(9): 0.0; T(10): 100.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (M4)","Search.ID":"M","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSSPLHRLSPTGK","Annotated.Sequence":"[R].mSsPLHRLsPTGK.[S]","Modifications":"M1(Oxidation); S3(Phospho); S9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.4383,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":529.56452,"Contaminant":"False","Aligned.m.z..Da.":529.56455,"MH...Da.":1586.679,"Theo..MH...Da.":1586.67977,"DeltaM..ppm.":-0.49,"Deltam.z..Da.":-0.00026,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2935170.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":47.97359,"Ion.Inject.Time..ms.":22,"RT..min.":7.673,"First.Scan":5783,"Last.Scan":5783,"Master.Scan.s.":5778,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_06.raw","File.ID":"F24","Quan.Info":"","Peptides.Matched":979,"XCorr":3.24,"X..Protein.Groups":1,"Percolator.q.Value":0.00048,"Percolator.PEP":0.00016,"Percolator.SVMScore":1.368,"Precursor.Area":6143522,"Apex.RT..min.":7.7,"ptmRS..Binomial.Peptide.Score":195.65,"ptmRS..Isoform.Confidence.Probability":0.99,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(8): 877.50; y2+-H(3) O(4) P(10): 544.32; y2+-H(3) O(4) P(8): 439.25; y1+(4): 402.23","ptmRS..Best.Site.Probabilities":"S3(Phospho): 100; S9(Phospho): 99.03","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(3): 100.0; S(9): 99.0; T(11): 1.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (M4)","Search.ID":"M","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LQETNPEEVPK","Annotated.Sequence":"[K].LQETNPEEVPK.[F]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":3,"DeltaScore":0.3951,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":642.32753,"Contaminant":"False","Aligned.m.z..Da.":642.32757,"MH...Da.":1283.64779,"Theo..MH...Da.":1283.64777,"DeltaM..ppm.":0.01,"Deltam.z..Da.":1e-05,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3599510.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":68.89575,"Ion.Inject.Time..ms.":17.877,"RT..min.":7.6529,"First.Scan":5755,"Last.Scan":5755,"Master.Scan.s.":5752,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_06.raw","File.ID":"F24","Quan.Info":"","Peptides.Matched":526,"XCorr":1.62,"X..Protein.Groups":1,"Percolator.q.Value":0.00048,"Percolator.PEP":0.00037,"Percolator.SVMScore":1.211,"Precursor.Area":7577314.5,"Apex.RT..min.":7.63,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (M4)","Search.ID":"M","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LQETNPEEVPK","Annotated.Sequence":"[K].LQETNPEEVPK.[F]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.55,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":642.32767,"Contaminant":"False","Aligned.m.z..Da.":642.32771,"MH...Da.":1283.64807,"Theo..MH...Da.":1283.64777,"DeltaM..ppm.":0.23,"Deltam.z..Da.":0.00015,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3286492.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":7.49928,"Ion.Inject.Time..ms.":22,"RT..min.":7.6076,"First.Scan":5693,"Last.Scan":5693,"Master.Scan.s.":5687,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_06.raw","File.ID":"F24","Quan.Info":"","Peptides.Matched":527,"XCorr":2.4,"X..Protein.Groups":1,"Percolator.q.Value":0.00048,"Percolator.PEP":0.00006,"Percolator.SVMScore":1.555,"Precursor.Area":7577314.5,"Apex.RT..min.":7.63,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (M4)","Search.ID":"M","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"IQDPQMTGYVSTR","Annotated.Sequence":"[R].IQDPQmtGYVsTR.[Y]","Modifications":"M6(Oxidation); T7(Phospho); S11(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P32485","Master.Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","Protein.Accessions":"P32485","Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.0612,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":836.32577,"Contaminant":"False","Aligned.m.z..Da.":836.32582,"MH...Da.":1671.64426,"Theo..MH...Da.":1671.64853,"DeltaM..ppm.":-2.55,"Deltam.z..Da.":-0.00214,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2633086.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":44.81685,"Ion.Inject.Time..ms.":22,"RT..min.":7.6937,"First.Scan":5812,"Last.Scan":5812,"Master.Scan.s.":5804,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_06.raw","File.ID":"F24","Quan.Info":"","Peptides.Matched":689,"XCorr":2.45,"X..Protein.Groups":1,"Percolator.q.Value":0.00048,"Percolator.PEP":0.00029,"Percolator.SVMScore":1.258,"Precursor.Area":2725992.75,"Apex.RT..min.":7.71,"ptmRS..Binomial.Peptide.Score":277.56,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(11): 1332.53; y1+-H(3) O(4) P(10): 1217.50; y1+-H(3) O(4) P(8): 992.39; y1+-H(3) O(4) P(7): 845.36; y2+-H(3) O(4) P(11): 666.77; y2+-H(3) O(4) P(10): 609.25; y1+(4): 462.27; y1+(3): 363.20","ptmRS..Best.Site.Probabilities":"T7(Phospho): 100; Y9(Phospho): 99.99","ptmRS..Phospho.Site.Probabilities":"T(7): 100.0; Y(9): 100.0; S(11): 0.0; T(12): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (M4)","Search.ID":"M","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DFLLKPELSR","Annotated.Sequence":"[K].DFLLKPELSR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.5795,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":406.56689,"Contaminant":"False","Aligned.m.z..Da.":406.56693,"MH...Da.":1217.68612,"Theo..MH...Da.":1217.68885,"DeltaM..ppm.":-2.24,"Deltam.z..Da.":-0.00091,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":982820.8125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":49.45757,"Ion.Inject.Time..ms.":22,"RT..min.":13.1718,"First.Scan":13181,"Last.Scan":13181,"Master.Scan.s.":13175,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_06.raw","File.ID":"F24","Quan.Info":"","Peptides.Matched":238,"XCorr":1.95,"X..Protein.Groups":1,"Percolator.q.Value":0.00048,"Percolator.PEP":0.00004,"Percolator.SVMScore":1.655,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (R4)","Search.ID":"R","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"VFDNIQR","Annotated.Sequence":"[R].VFDNIQR.[R]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.4247,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":446.23836,"Contaminant":"False","Aligned.m.z..Da.":446.23841,"MH...Da.":891.46945,"Theo..MH...Da.":891.46829,"DeltaM..ppm.":1.3,"Deltam.z..Da.":0.00058,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":5562218.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":41.31405,"Ion.Inject.Time..ms.":16.076,"RT..min.":8.2576,"First.Scan":6229,"Last.Scan":6229,"Master.Scan.s.":6217,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_01.raw","File.ID":"F19","Quan.Info":"","Peptides.Matched":363,"XCorr":1.86,"X..Protein.Groups":1,"Percolator.q.Value":0.00048,"Percolator.PEP":0.00863,"Percolator.SVMScore":0.617,"Precursor.Area":4242516,"Apex.RT..min.":8.26,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (R4)","Search.ID":"R","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"VFDNIQR","Annotated.Sequence":"[R].VFDNIQR.[R]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.4171,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":446.23849,"Contaminant":"False","Aligned.m.z..Da.":446.23853,"MH...Da.":891.4697,"Theo..MH...Da.":891.46829,"DeltaM..ppm.":1.58,"Deltam.z..Da.":0.0007,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2458851.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":54.77255,"Ion.Inject.Time..ms.":22,"RT..min.":8.2433,"First.Scan":6211,"Last.Scan":6211,"Master.Scan.s.":6204,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_01.raw","File.ID":"F19","Quan.Info":"","Peptides.Matched":362,"XCorr":1.99,"X..Protein.Groups":1,"Percolator.q.Value":0.00048,"Percolator.PEP":0.00881,"Percolator.SVMScore":0.613,"Precursor.Area":4242516,"Apex.RT..min.":8.26,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (R4)","Search.ID":"R","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LQQTAFDKK","Annotated.Sequence":"[R].LQQTAFDKK.[S]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.4502,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":360.20156,"Contaminant":"False","Aligned.m.z..Da.":360.20158,"MH...Da.":1078.59014,"Theo..MH...Da.":1078.58914,"DeltaM..ppm.":0.93,"Deltam.z..Da.":0.00034,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":4622210.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":32.06417,"Ion.Inject.Time..ms.":17.061,"RT..min.":5.4861,"First.Scan":2286,"Last.Scan":2286,"Master.Scan.s.":2278,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_01.raw","File.ID":"F19","Quan.Info":"","Peptides.Matched":392,"XCorr":2.71,"X..Protein.Groups":1,"Percolator.q.Value":0.00048,"Percolator.PEP":0.00229,"Percolator.SVMScore":0.867,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (R4)","Search.ID":"R","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ELDEIAASLENL","Annotated.Sequence":"[K].ELDEIAAsLENL.[-]","Modifications":"S8(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.692,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":698.81595,"Contaminant":"False","Aligned.m.z..Da.":698.81619,"MH...Da.":1396.62463,"Theo..MH...Da.":1396.62433,"DeltaM..ppm.":0.21,"Deltam.z..Da.":0.00015,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1104721.625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":18.2255,"First.Scan":18677,"Last.Scan":18677,"Master.Scan.s.":18672,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_01.raw","File.ID":"F19","Quan.Info":"","Peptides.Matched":873,"XCorr":2.63,"X..Protein.Groups":1,"Percolator.q.Value":0.00048,"Percolator.PEP":0.00392,"Percolator.SVMScore":0.766,"Precursor.Area":1062336.375,"Apex.RT..min.":18.23,"ptmRS..Binomial.Peptide.Score":188.44,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"S8(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(8): 100.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Q4)","Search.ID":"Q","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KDELTLEGIK","Annotated.Sequence":"[K].KDELTLEGIK.[Q]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.5487,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":382.55179,"Contaminant":"False","Aligned.m.z..Da.":382.55182,"MH...Da.":1145.64081,"Theo..MH...Da.":1145.64123,"DeltaM..ppm.":-0.37,"Deltam.z..Da.":-0.00014,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2417684.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":1.7342,"Ion.Inject.Time..ms.":22,"RT..min.":10.2602,"First.Scan":8617,"Last.Scan":8617,"Master.Scan.s.":8611,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_02.raw","File.ID":"F20","Quan.Info":"","Peptides.Matched":314,"XCorr":2.77,"X..Protein.Groups":1,"Percolator.q.Value":0.00048,"Percolator.PEP":0.00051,"Percolator.SVMScore":1.24,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Q4)","Search.ID":"Q","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ILISTDLLAR","Annotated.Sequence":"[R].ILISTDLLAR.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5128,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":557.84544,"Contaminant":"False","Aligned.m.z..Da.":557.84551,"MH...Da.":1114.68362,"Theo..MH...Da.":1114.68304,"DeltaM..ppm.":0.52,"Deltam.z..Da.":0.00029,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":341166.59375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":53.08836,"Ion.Inject.Time..ms.":22,"RT..min.":15.6391,"First.Scan":15576,"Last.Scan":15576,"Master.Scan.s.":15575,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_02.raw","File.ID":"F20","Quan.Info":"","Peptides.Matched":124,"XCorr":1.56,"X..Protein.Groups":1,"Percolator.q.Value":0.00048,"Percolator.PEP":0.00465,"Percolator.SVMScore":0.799,"Precursor.Area":1887916.625,"Apex.RT..min.":15.6,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Q4)","Search.ID":"Q","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"GVFGYGFEEPSAIQQR","Annotated.Sequence":"[R].GVFGYGFEEPSAIQQR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.5417,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":595.62504,"Contaminant":"False","Aligned.m.z..Da.":595.62518,"MH...Da.":1784.86058,"Theo..MH...Da.":1784.86022,"DeltaM..ppm.":0.2,"Deltam.z..Da.":0.00012,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":412167.6875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":17.6661,"Ion.Inject.Time..ms.":22,"RT..min.":15.0371,"First.Scan":14830,"Last.Scan":14830,"Master.Scan.s.":14818,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_02.raw","File.ID":"F20","Quan.Info":"","Peptides.Matched":1369,"XCorr":2.16,"X..Protein.Groups":1,"Percolator.q.Value":0.00048,"Percolator.PEP":0.00152,"Percolator.SVMScore":1.022,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Q4)","Search.ID":"Q","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MGSINNNFNTNNNSNTDLDR","Annotated.Sequence":"[-].mGSINNNFNTNNNsNTDLDR.[D]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S14(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P39517","Master.Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","Protein.Accessions":"P39517","Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.1544,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":749.30969,"Contaminant":"False","Aligned.m.z..Da.":749.30972,"MH...Da.":2245.91454,"Theo..MH...Da.":2245.91057,"DeltaM..ppm.":1.77,"Deltam.z..Da.":0.00132,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":552003.1875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":40.54199,"Ion.Inject.Time..ms.":22,"RT..min.":11.9924,"First.Scan":10904,"Last.Scan":10904,"Master.Scan.s.":10899,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_02.raw","File.ID":"F20","Quan.Info":"","Peptides.Matched":1457,"XCorr":2.59,"X..Protein.Groups":1,"Percolator.q.Value":0.00048,"Percolator.PEP":0.00046,"Percolator.SVMScore":1.263,"Precursor.Area":511127.0625,"Apex.RT..min.":11.99,"ptmRS..Binomial.Peptide.Score":193.57,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(12): 1359.59; y1+-H(3) O(4) P(9): 1030.45; y1+-H(3) O(4) P(8): 916.41; y1+-H(3) O(4) P(7): 802.37; y1+(6): 733.35; y1+(5): 619.30","ptmRS..Best.Site.Probabilities":"S14(Phospho): 99.99","ptmRS..Phospho.Site.Probabilities":"S(3): 0.0; T(10): 0.0; S(14): 100.0; T(16): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Q4)","Search.ID":"Q","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSSPLHRLSPTGK","Annotated.Sequence":"[R].mSsPLHRLsPTGK.[S]","Modifications":"M1(Oxidation); S3(Phospho); S9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5805,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":793.84243,"Contaminant":"False","Aligned.m.z..Da.":793.84254,"MH...Da.":1586.67759,"Theo..MH...Da.":1586.67977,"DeltaM..ppm.":-1.38,"Deltam.z..Da.":-0.00109,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1080624.625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":15.67235,"Ion.Inject.Time..ms.":22,"RT..min.":7.7497,"First.Scan":5597,"Last.Scan":5597,"Master.Scan.s.":5595,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_02.raw","File.ID":"F20","Quan.Info":"","Peptides.Matched":976,"XCorr":1.74,"X..Protein.Groups":1,"Percolator.q.Value":0.00048,"Percolator.PEP":0.00046,"Percolator.SVMScore":1.261,"Precursor.Area":1642339.375,"Apex.RT..min.":7.73,"ptmRS..Binomial.Peptide.Score":327.67,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(3): 304.10; b2+-H(3) O(4) P(9): 544.24; y2+-H(3) O(4) P(11): 627.82; y2+- -2H(3) O(4) P -2H(3) O(4) P(11): 578.83; b1+(2): 235.07","ptmRS..Best.Site.Probabilities":"S3(Phospho): 100; S9(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(3): 100.0; S(9): 100.0; T(11): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Q4)","Search.ID":"Q","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KFQDDTLNR","Annotated.Sequence":"[K].KFQDDtLNR.[V]","Modifications":"T6(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6772,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":608.77221,"Contaminant":"False","Aligned.m.z..Da.":608.77221,"MH...Da.":1216.53715,"Theo..MH...Da.":1216.53579,"DeltaM..ppm.":1.12,"Deltam.z..Da.":0.00068,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3058867.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":17.21051,"Ion.Inject.Time..ms.":22,"RT..min.":6.2028,"First.Scan":3128,"Last.Scan":3128,"Master.Scan.s.":3125,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_02.raw","File.ID":"F20","Quan.Info":"","Peptides.Matched":613,"XCorr":3.16,"X..Protein.Groups":1,"Percolator.q.Value":0.00048,"Percolator.PEP":0.00084,"Percolator.SVMScore":1.142,"Precursor.Area":1876080.375,"Apex.RT..min.":6.2,"ptmRS..Binomial.Peptide.Score":336.03,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"T6(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"T(6): 100.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Q4)","Search.ID":"Q","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"IGNNELLHR","Annotated.Sequence":"[K].IGNNELLHR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q03558","Master.Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","Protein.Accessions":"Q03558","Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.4774,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":355.86495,"Contaminant":"False","Aligned.m.z..Da.":355.86499,"MH...Da.":1065.58031,"Theo..MH...Da.":1065.57997,"DeltaM..ppm.":0.32,"Deltam.z..Da.":0.00011,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2202506.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":14.63783,"Ion.Inject.Time..ms.":22,"RT..min.":7.5725,"First.Scan":5340,"Last.Scan":5340,"Master.Scan.s.":5335,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_02.raw","File.ID":"F20","Quan.Info":"","Peptides.Matched":300,"XCorr":1.99,"X..Protein.Groups":1,"Percolator.q.Value":0.00048,"Percolator.PEP":0.00188,"Percolator.SVMScore":0.979,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Q4)","Search.ID":"Q","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LQQTAFDKK","Annotated.Sequence":"[R].LQQTAFDKK.[S]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.3224,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":360.2011,"Contaminant":"False","Aligned.m.z..Da.":360.20113,"MH...Da.":1078.58874,"Theo..MH...Da.":1078.58914,"DeltaM..ppm.":-0.37,"Deltam.z..Da.":-0.00013,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3579169.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":56.6825,"Ion.Inject.Time..ms.":13.163,"RT..min.":5.493,"First.Scan":2249,"Last.Scan":2249,"Master.Scan.s.":2241,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_02.raw","File.ID":"F20","Quan.Info":"","Peptides.Matched":392,"XCorr":2.14,"X..Protein.Groups":1,"Percolator.q.Value":0.00048,"Percolator.PEP":0.00516,"Percolator.SVMScore":0.778,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Q4)","Search.ID":"Q","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LQQTAFDKK","Annotated.Sequence":"[R].LQQTAFDKK.[S]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":4,"DeltaScore":0.4361,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":360.20115,"Contaminant":"False","Aligned.m.z..Da.":360.20119,"MH...Da.":1078.58891,"Theo..MH...Da.":1078.58914,"DeltaM..ppm.":-0.21,"Deltam.z..Da.":-0.00007,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3056487.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":53.66954,"Ion.Inject.Time..ms.":18.59,"RT..min.":5.501,"First.Scan":2256,"Last.Scan":2256,"Master.Scan.s.":2254,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_02.raw","File.ID":"F20","Quan.Info":"","Peptides.Matched":392,"XCorr":2.27,"X..Protein.Groups":1,"Percolator.q.Value":0.00048,"Percolator.PEP":0.00245,"Percolator.SVMScore":0.927,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (O4)","Search.ID":"O","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"IGNNELLHR","Annotated.Sequence":"[K].IGNNELLHR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q03558","Master.Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","Protein.Accessions":"Q03558","Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.4216,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":355.86495,"Contaminant":"False","Aligned.m.z..Da.":355.86497,"MH...Da.":1065.58029,"Theo..MH...Da.":1065.57997,"DeltaM..ppm.":0.3,"Deltam.z..Da.":0.00011,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1696528.625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":10.48214,"Ion.Inject.Time..ms.":22,"RT..min.":7.57,"First.Scan":5319,"Last.Scan":5319,"Master.Scan.s.":5308,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_04.raw","File.ID":"F22","Quan.Info":"","Peptides.Matched":300,"XCorr":2.04,"X..Protein.Groups":1,"Percolator.q.Value":0.00048,"Percolator.PEP":0.00216,"Percolator.SVMScore":0.864,"Precursor.Area":1200996.375,"Apex.RT..min.":7.57,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (O4)","Search.ID":"O","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DFLLKPELSR","Annotated.Sequence":"[K].DFLLKPELSR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.623,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":406.56776,"Contaminant":"False","Aligned.m.z..Da.":406.56789,"MH...Da.":1217.68873,"Theo..MH...Da.":1217.68885,"DeltaM..ppm.":-0.09,"Deltam.z..Da.":-0.00004,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":669561.0625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":3.34228,"Ion.Inject.Time..ms.":22,"RT..min.":13.149,"First.Scan":12478,"Last.Scan":12478,"Master.Scan.s.":12471,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_04.raw","File.ID":"F22","Quan.Info":"","Peptides.Matched":265,"XCorr":2.44,"X..Protein.Groups":1,"Percolator.q.Value":0.00048,"Percolator.PEP":0.00006,"Percolator.SVMScore":1.555,"Precursor.Area":526835.1875,"Apex.RT..min.":13.15,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (O4)","Search.ID":"O","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"APQALMLAPTR","Annotated.Sequence":"[K].APQALMLAPTR.[E]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":3,"DeltaScore":0.594,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":584.82949,"Contaminant":"False","Aligned.m.z..Da.":584.82954,"MH...Da.":1168.65169,"Theo..MH...Da.":1168.65069,"DeltaM..ppm.":0.86,"Deltam.z..Da.":0.0005,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":322921.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":92.0473,"Ion.Inject.Time..ms.":22,"RT..min.":11.6357,"First.Scan":10420,"Last.Scan":10420,"Master.Scan.s.":10417,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_04.raw","File.ID":"F22","Quan.Info":"","Peptides.Matched":202,"XCorr":1.33,"X..Protein.Groups":1,"Percolator.q.Value":0.00048,"Percolator.PEP":0.0001,"Percolator.SVMScore":1.438,"Precursor.Area":539600.9375,"Apex.RT..min.":11.61,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (O4)","Search.ID":"O","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KFQDDTLNR","Annotated.Sequence":"[K].KFQDDtLNR.[V]","Modifications":"T6(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7036,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":608.77243,"Contaminant":"False","Aligned.m.z..Da.":608.77239,"MH...Da.":1216.53758,"Theo..MH...Da.":1216.53579,"DeltaM..ppm.":1.47,"Deltam.z..Da.":0.00089,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1420629.875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":24.26532,"Ion.Inject.Time..ms.":22,"RT..min.":6.2,"First.Scan":3208,"Last.Scan":3208,"Master.Scan.s.":3202,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_04.raw","File.ID":"F22","Quan.Info":"","Peptides.Matched":606,"XCorr":3.07,"X..Protein.Groups":1,"Percolator.q.Value":0.00048,"Percolator.PEP":0.00004,"Percolator.SVMScore":1.631,"Precursor.Area":1936464.25,"Apex.RT..min.":6.21,"ptmRS..Binomial.Peptide.Score":360.02,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"T6(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"T(6): 100.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (O4)","Search.ID":"O","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MFILDEADEMLSSGFK","Annotated.Sequence":"[K].mFILDEADEmLSSGFK.[E]","Modifications":"M1(Oxidation); M10(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.6642,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":622.28352,"Contaminant":"False","Aligned.m.z..Da.":622.28342,"MH...Da.":1864.83602,"Theo..MH...Da.":1864.83433,"DeltaM..ppm.":0.9,"Deltam.z..Da.":0.00056,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":327542.78125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":16.6948,"First.Scan":16973,"Last.Scan":16973,"Master.Scan.s.":16961,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_04.raw","File.ID":"F22","Quan.Info":"","Peptides.Matched":1893,"XCorr":2.65,"X..Protein.Groups":1,"Percolator.q.Value":0.00048,"Percolator.PEP":0.00006,"Percolator.SVMScore":1.554,"Precursor.Area":299357.625,"Apex.RT..min.":16.79,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (O4)","Search.ID":"O","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ELALQIQK","Annotated.Sequence":"[R].ELALQIQK.[V]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.2394,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":471.78514,"Contaminant":"False","Aligned.m.z..Da.":471.78519,"MH...Da.":942.563,"Theo..MH...Da.":942.56186,"DeltaM..ppm.":1.21,"Deltam.z..Da.":0.00057,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":795847.0625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":42.18074,"Ion.Inject.Time..ms.":22,"RT..min.":10.6159,"First.Scan":9106,"Last.Scan":9106,"Master.Scan.s.":9104,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_04.raw","File.ID":"F22","Quan.Info":"","Peptides.Matched":292,"XCorr":1.88,"X..Protein.Groups":1,"Percolator.q.Value":0.00048,"Percolator.PEP":0.00336,"Percolator.SVMScore":0.782,"Precursor.Area":2963715.5,"Apex.RT..min.":10.59,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (O4)","Search.ID":"O","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSSPLHRLSPTGK","Annotated.Sequence":"[R].mSsPLHRLsPTGK.[S]","Modifications":"M1(Oxidation); S3(Phospho); S9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.4844,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":529.56468,"Contaminant":"False","Aligned.m.z..Da.":529.56472,"MH...Da.":1586.67948,"Theo..MH...Da.":1586.67977,"DeltaM..ppm.":-0.18,"Deltam.z..Da.":-0.0001,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3062705.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":53.86539,"Ion.Inject.Time..ms.":22,"RT..min.":7.7147,"First.Scan":5525,"Last.Scan":5525,"Master.Scan.s.":5516,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_04.raw","File.ID":"F22","Quan.Info":"","Peptides.Matched":1019,"XCorr":3.53,"X..Protein.Groups":1,"Percolator.q.Value":0.00048,"Percolator.PEP":0.0001,"Percolator.SVMScore":1.452,"Precursor.Area":6032152.5,"Apex.RT..min.":7.74,"ptmRS..Binomial.Peptide.Score":285.05,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(8): 877.50; y2+-H(3) O(4) P(12): 671.34; y2+-H(3) O(4) P(11): 627.82; y2+-H(3) O(4) P(10): 544.32; y2+-H(3) O(4) P(8): 439.25; y1+(4): 402.23; y1+(3): 305.18","ptmRS..Best.Site.Probabilities":"S3(Phospho): 100; S9(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(3): 100.0; S(9): 100.0; T(11): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (O4)","Search.ID":"O","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KDELTLEGIK","Annotated.Sequence":"[K].KDELTLEGIK.[Q]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.5502,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":382.55163,"Contaminant":"False","Aligned.m.z..Da.":382.55166,"MH...Da.":1145.64034,"Theo..MH...Da.":1145.64123,"DeltaM..ppm.":-0.78,"Deltam.z..Da.":-0.0003,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1588949,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":48.39706,"Ion.Inject.Time..ms.":22,"RT..min.":10.2561,"First.Scan":8661,"Last.Scan":8661,"Master.Scan.s.":8649,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_04.raw","File.ID":"F22","Quan.Info":"","Peptides.Matched":351,"XCorr":2.69,"X..Protein.Groups":1,"Percolator.q.Value":0.00048,"Percolator.PEP":0.00014,"Percolator.SVMScore":1.377,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (O4)","Search.ID":"O","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"IQDPQMTGYVSTR","Annotated.Sequence":"[R].IQDPQmtGYVsTR.[Y]","Modifications":"M6(Oxidation); T7(Phospho); S11(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P32485","Master.Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","Protein.Accessions":"P32485","Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.0323,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":836.32797,"Contaminant":"False","Aligned.m.z..Da.":836.32804,"MH...Da.":1671.64866,"Theo..MH...Da.":1671.64853,"DeltaM..ppm.":0.08,"Deltam.z..Da.":0.00007,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2084717,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":22.76795,"Ion.Inject.Time..ms.":22,"RT..min.":7.7446,"First.Scan":5567,"Last.Scan":5567,"Master.Scan.s.":5555,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_04.raw","File.ID":"F22","Quan.Info":"","Peptides.Matched":762,"XCorr":2.48,"X..Protein.Groups":1,"Percolator.q.Value":0.00048,"Percolator.PEP":0.00009,"Percolator.SVMScore":1.467,"Precursor.Area":2365018,"Apex.RT..min.":7.76,"ptmRS..Binomial.Peptide.Score":340.54,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(11): 1332.53; y1+-H(3) O(4) P(10): 1217.50; y1+-H(3) O(4) P(8): 992.39; y1+-H(3) O(4) P(7): 845.36; y2+-H(3) O(4) P(11): 666.77; y2+-H(3) O(4) P(10): 609.25; y1+(4): 462.27; y1+(3): 363.20","ptmRS..Best.Site.Probabilities":"T7(Phospho): 100; Y9(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"T(7): 100.0; Y(9): 100.0; S(11): 0.0; T(12): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (O4)","Search.ID":"O","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSSPLHRLSPTGK","Annotated.Sequence":"[R].mSsPLHRLsPTGK.[S]","Modifications":"M1(Oxidation); S3(Phospho); S9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.477,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":793.8441,"Contaminant":"False","Aligned.m.z..Da.":793.84417,"MH...Da.":1586.68094,"Theo..MH...Da.":1586.67977,"DeltaM..ppm.":0.73,"Deltam.z..Da.":0.00058,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2236080.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":10.63848,"Ion.Inject.Time..ms.":22,"RT..min.":7.744,"First.Scan":5566,"Last.Scan":5566,"Master.Scan.s.":5555,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_04.raw","File.ID":"F22","Quan.Info":"","Peptides.Matched":1094,"XCorr":2.39,"X..Protein.Groups":1,"Percolator.q.Value":0.00048,"Percolator.PEP":0.00005,"Percolator.SVMScore":1.571,"Precursor.Area":1864990.875,"Apex.RT..min.":7.73,"ptmRS..Binomial.Peptide.Score":280.29,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(3): 304.10; y2+-H(3) O(4) P(11): 627.82; y2+- -2H(3) O(4) P -2H(3) O(4) P(11): 578.83; b1+(2): 235.07","ptmRS..Best.Site.Probabilities":"S3(Phospho): 100; S9(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(3): 100.0; S(9): 100.0; T(11): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (O4)","Search.ID":"O","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ELDEIAASLENL","Annotated.Sequence":"[K].ELDEIAAsLENL.[-]","Modifications":"S8(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7422,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":698.81581,"Contaminant":"False","Aligned.m.z..Da.":698.81609,"MH...Da.":1396.62434,"Theo..MH...Da.":1396.62433,"DeltaM..ppm.":0.01,"Deltam.z..Da.":1e-05,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1128843.125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":18.2411,"First.Scan":18954,"Last.Scan":18954,"Master.Scan.s.":18950,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_04.raw","File.ID":"F22","Quan.Info":"","Peptides.Matched":879,"XCorr":2.87,"X..Protein.Groups":1,"Percolator.q.Value":0.00048,"Percolator.PEP":0.00116,"Percolator.SVMScore":0.982,"Precursor.Area":1260895,"Apex.RT..min.":18.25,"ptmRS..Binomial.Peptide.Score":208.35,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"S8(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(8): 100.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (O4)","Search.ID":"O","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ELDEIAASLENL","Annotated.Sequence":"[K].ELDEIAASLENL.[-]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6017,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":658.83342,"Contaminant":"False","Aligned.m.z..Da.":658.83369,"MH...Da.":1316.65958,"Theo..MH...Da.":1316.658,"DeltaM..ppm.":1.2,"Deltam.z..Da.":0.00079,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":751556,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":30.6795,"Ion.Inject.Time..ms.":22,"RT..min.":18.4543,"First.Scan":19233,"Last.Scan":19233,"Master.Scan.s.":19223,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_04.raw","File.ID":"F22","Quan.Info":"","Peptides.Matched":656,"XCorr":2.41,"X..Protein.Groups":1,"Percolator.q.Value":0.00048,"Percolator.PEP":0.00088,"Percolator.SVMScore":1.033,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (O4)","Search.ID":"O","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"NHQLEEEIEK","Annotated.Sequence":"[K].NHQLEEEIEK.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.4774,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":634.81039,"Contaminant":"False","Aligned.m.z..Da.":634.81038,"MH...Da.":1268.6135,"Theo..MH...Da.":1268.61172,"DeltaM..ppm.":1.4,"Deltam.z..Da.":0.00089,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":801227.3125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":76.3478,"Ion.Inject.Time..ms.":22,"RT..min.":7.1712,"First.Scan":4715,"Last.Scan":4715,"Master.Scan.s.":4710,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_04.raw","File.ID":"F22","Quan.Info":"","Peptides.Matched":877,"XCorr":1.55,"X..Protein.Groups":1,"Percolator.q.Value":0.00048,"Percolator.PEP":0.00326,"Percolator.SVMScore":0.787,"Precursor.Area":558578.6875,"Apex.RT..min.":7.17,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (O4)","Search.ID":"O","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"LEAGLSDSK","Annotated.Sequence":"[K].LEAGLsDSK.[Q]","Modifications":"S6(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.0463,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":500.22415,"Contaminant":"False","Aligned.m.z..Da.":500.22418,"MH...Da.":999.44102,"Theo..MH...Da.":999.43943,"DeltaM..ppm.":1.59,"Deltam.z..Da.":0.00079,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2311819.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":30.21726,"Ion.Inject.Time..ms.":22,"RT..min.":7.575,"First.Scan":5325,"Last.Scan":5325,"Master.Scan.s.":5321,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_04.raw","File.ID":"F22","Quan.Info":"","Peptides.Matched":748,"XCorr":2.59,"X..Protein.Groups":1,"Percolator.q.Value":0.00048,"Percolator.PEP":0.00085,"Percolator.SVMScore":1.041,"Precursor.Area":2994219,"Apex.RT..min.":7.59,"ptmRS..Binomial.Peptide.Score":219.95,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(8): 788.38; y1+-H(3) O(4) P(7): 659.34; y1+-H(3) O(4) P(6): 588.30; y1+-H(3) O(4) P(5): 531.28; y1+-H(3) O(4) P(4): 418.19; y1+(3): 349.17; y1+(2): 234.14","ptmRS..Best.Site.Probabilities":"S6(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(6): 100.0; S(8): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (O4)","Search.ID":"O","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ILISTDLLAR","Annotated.Sequence":"[R].ILISTDLLAR.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5165,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":557.84564,"Contaminant":"False","Aligned.m.z..Da.":557.84559,"MH...Da.":1114.684,"Theo..MH...Da.":1114.68304,"DeltaM..ppm.":0.87,"Deltam.z..Da.":0.00048,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1526095.625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":18.03568,"Ion.Inject.Time..ms.":22,"RT..min.":15.5824,"First.Scan":15583,"Last.Scan":15583,"Master.Scan.s.":15571,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_04.raw","File.ID":"F22","Quan.Info":"","Peptides.Matched":124,"XCorr":2.73,"X..Protein.Groups":1,"Percolator.q.Value":0.00048,"Percolator.PEP":0.00012,"Percolator.SVMScore":1.413,"Precursor.Area":2082964.125,"Apex.RT..min.":15.59,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (O4)","Search.ID":"O","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ILISTDLLAR","Annotated.Sequence":"[R].ILISTDLLAR.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5241,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":557.8465,"Contaminant":"False","Aligned.m.z..Da.":557.84644,"MH...Da.":1114.68573,"Theo..MH...Da.":1114.68304,"DeltaM..ppm.":2.41,"Deltam.z..Da.":0.00135,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":224801.34375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":37.5715,"Ion.Inject.Time..ms.":22,"RT..min.":15.6376,"First.Scan":15650,"Last.Scan":15650,"Master.Scan.s.":15649,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_04.raw","File.ID":"F22","Quan.Info":"","Peptides.Matched":126,"XCorr":1.66,"X..Protein.Groups":1,"Percolator.q.Value":0.00048,"Percolator.PEP":0.00469,"Percolator.SVMScore":0.719,"Precursor.Area":2082964.125,"Apex.RT..min.":15.59,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (O4)","Search.ID":"O","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FFISNPDLVDRLEK","Annotated.Sequence":"[R].FFISNPDLVDRLEK.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q03558","Master.Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","Protein.Accessions":"Q03558","Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.6829,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":564.9699,"Contaminant":"False","Aligned.m.z..Da.":564.96987,"MH...Da.":1692.89516,"Theo..MH...Da.":1692.89555,"DeltaM..ppm.":-0.23,"Deltam.z..Da.":-0.00013,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":480090.90625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":41.18778,"Ion.Inject.Time..ms.":22,"RT..min.":15.3438,"First.Scan":15284,"Last.Scan":15284,"Master.Scan.s.":15272,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_04.raw","File.ID":"F22","Quan.Info":"","Peptides.Matched":560,"XCorr":2.05,"X..Protein.Groups":1,"Percolator.q.Value":0.00048,"Percolator.PEP":0.00021,"Percolator.SVMScore":1.301,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (O4)","Search.ID":"O","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"TAVFYGGTPISK","Annotated.Sequence":"[K].TAVFYGGTPISK.[D]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5105,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":620.83239,"Contaminant":"False","Aligned.m.z..Da.":620.83246,"MH...Da.":1240.6575,"Theo..MH...Da.":1240.65721,"DeltaM..ppm.":0.23,"Deltam.z..Da.":0.00014,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":492886.21875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":78.76334,"Ion.Inject.Time..ms.":22,"RT..min.":11.1267,"First.Scan":9751,"Last.Scan":9751,"Master.Scan.s.":9741,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_04.raw","File.ID":"F22","Quan.Info":"","Peptides.Matched":385,"XCorr":2.39,"X..Protein.Groups":1,"Percolator.q.Value":0.00048,"Percolator.PEP":0.00005,"Percolator.SVMScore":1.566,"Precursor.Area":445141.40625,"Apex.RT..min.":11.13,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (K4)","Search.ID":"K","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ILISTDLLAR","Annotated.Sequence":"[R].ILISTDLLAR.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5212,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":557.84569,"Contaminant":"False","Aligned.m.z..Da.":557.84563,"MH...Da.":1114.68411,"Theo..MH...Da.":1114.68304,"DeltaM..ppm.":0.96,"Deltam.z..Da.":0.00054,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1293095.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":25.32416,"Ion.Inject.Time..ms.":22,"RT..min.":15.586,"First.Scan":15571,"Last.Scan":15571,"Master.Scan.s.":15565,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_02.raw","File.ID":"F26","Quan.Info":"","Peptides.Matched":124,"XCorr":2.59,"X..Protein.Groups":1,"Percolator.q.Value":0.00048,"Percolator.PEP":0.00002,"Percolator.SVMScore":1.831,"Precursor.Area":2224160.25,"Apex.RT..min.":15.61,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (K4)","Search.ID":"K","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ILISTDLLAR","Annotated.Sequence":"[R].ILISTDLLAR.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5023,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":557.84521,"Contaminant":"False","Aligned.m.z..Da.":557.84514,"MH...Da.":1114.68314,"Theo..MH...Da.":1114.68304,"DeltaM..ppm.":0.1,"Deltam.z..Da.":0.00005,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":954144.4375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":41.1324,"Ion.Inject.Time..ms.":22,"RT..min.":15.6339,"First.Scan":15631,"Last.Scan":15631,"Master.Scan.s.":15630,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_02.raw","File.ID":"F26","Quan.Info":"","Peptides.Matched":124,"XCorr":2.15,"X..Protein.Groups":1,"Percolator.q.Value":0.00048,"Percolator.PEP":0.00004,"Percolator.SVMScore":1.693,"Precursor.Area":2224160.25,"Apex.RT..min.":15.61,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (K4)","Search.ID":"K","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].msEGITDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S2(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1381,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1096.46536,"Contaminant":"False","Aligned.m.z..Da.":1096.46517,"MH...Da.":2191.92344,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":-2.01,"Deltam.z..Da.":-0.0022,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":236618.60938,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":81.09277,"Ion.Inject.Time..ms.":22,"RT..min.":16.3393,"First.Scan":16518,"Last.Scan":16518,"Master.Scan.s.":16511,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_02.raw","File.ID":"F26","Quan.Info":"","Peptides.Matched":1848,"XCorr":1.81,"X..Protein.Groups":1,"Percolator.q.Value":0.00048,"Percolator.PEP":0.00009,"Percolator.SVMScore":1.536,"Precursor.Area":594944.9375,"Apex.RT..min.":16.32,"ptmRS..Binomial.Peptide.Score":239.05,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(2): 112.04; b1+-H(3) O(4) P(3): 241.08; b1+-H(3) O(4) P(4): 298.10; b1+-H(3) O(4) P(5): 411.19; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+-H(3) O(4) P(9): 869.39; y1+(14): 1683.77","ptmRS..Best.Site.Probabilities":"S2(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 100.0; T(6): 0.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (K4)","Search.ID":"K","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ELALQIQK","Annotated.Sequence":"[R].ELALQIQK.[V]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.2035,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":471.78488,"Contaminant":"False","Aligned.m.z..Da.":471.78487,"MH...Da.":942.56248,"Theo..MH...Da.":942.56186,"DeltaM..ppm.":0.66,"Deltam.z..Da.":0.00031,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1453312.375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":47.58609,"Ion.Inject.Time..ms.":22,"RT..min.":10.6272,"First.Scan":9090,"Last.Scan":9090,"Master.Scan.s.":9078,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_02.raw","File.ID":"F26","Quan.Info":"","Peptides.Matched":292,"XCorr":1.72,"X..Protein.Groups":1,"Percolator.q.Value":0.00048,"Percolator.PEP":0.00059,"Percolator.SVMScore":1.18,"Precursor.Area":4172748.75,"Apex.RT..min.":10.59,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (K4)","Search.ID":"K","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"NHQLEEEIEK","Annotated.Sequence":"[K].NHQLEEEIEK.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.4727,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":634.81126,"Contaminant":"False","Aligned.m.z..Da.":634.81129,"MH...Da.":1268.61525,"Theo..MH...Da.":1268.61172,"DeltaM..ppm.":2.78,"Deltam.z..Da.":0.00177,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":881753.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":65.00648,"Ion.Inject.Time..ms.":22,"RT..min.":7.1795,"First.Scan":4705,"Last.Scan":4705,"Master.Scan.s.":4697,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_02.raw","File.ID":"F26","Quan.Info":"","Peptides.Matched":866,"XCorr":1.65,"X..Protein.Groups":1,"Percolator.q.Value":0.00048,"Percolator.PEP":0.00039,"Percolator.SVMScore":1.258,"Precursor.Area":741872.25,"Apex.RT..min.":7.17,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (K4)","Search.ID":"K","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEySDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); Y12(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0227,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40577,"Contaminant":"False","Aligned.m.z..Da.":967.40563,"MH...Da.":2900.20276,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":1.18,"Deltam.z..Da.":0.00114,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":147441.29688,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":68.2701,"Ion.Inject.Time..ms.":22,"RT..min.":16.1363,"First.Scan":16269,"Last.Scan":16269,"Master.Scan.s.":16267,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_02.raw","File.ID":"F26","Quan.Info":"","Peptides.Matched":2563,"XCorr":2.2,"X..Protein.Groups":1,"Percolator.q.Value":0.00048,"Percolator.PEP":0.00006,"Percolator.SVMScore":1.62,"ptmRS..Binomial.Peptide.Score":204.69,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"y2+-H(3) O(4) P(14): 811.37; b1+(12): 1344.56","ptmRS..Best.Site.Probabilities":"S13(Phospho): 99.98","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (K4)","Search.ID":"K","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KDELTLEGIK","Annotated.Sequence":"[K].KDELTLEGIK.[Q]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.562,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":382.55184,"Contaminant":"False","Aligned.m.z..Da.":382.55183,"MH...Da.":1145.64098,"Theo..MH...Da.":1145.64123,"DeltaM..ppm.":-0.21,"Deltam.z..Da.":-0.00008,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2686309.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":43.28671,"Ion.Inject.Time..ms.":22,"RT..min.":10.2608,"First.Scan":8633,"Last.Scan":8633,"Master.Scan.s.":8623,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_02.raw","File.ID":"F26","Quan.Info":"","Peptides.Matched":311,"XCorr":2.58,"X..Protein.Groups":1,"Percolator.q.Value":0.00048,"Percolator.PEP":0.00005,"Percolator.SVMScore":1.647,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (K4)","Search.ID":"K","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"EDGTTPFVAIWK","Annotated.Sequence":"[R].EDGTTPFVAIWK.[H]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.615,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":682.34751,"Contaminant":"False","Aligned.m.z..Da.":682.34732,"MH...Da.":1363.68775,"Theo..MH...Da.":1363.68924,"DeltaM..ppm.":-1.1,"Deltam.z..Da.":-0.00075,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":379278,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":10.19801,"Ion.Inject.Time..ms.":22,"RT..min.":16.9236,"First.Scan":17262,"Last.Scan":17262,"Master.Scan.s.":17252,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_02.raw","File.ID":"F26","Quan.Info":"","Peptides.Matched":652,"XCorr":2,"X..Protein.Groups":1,"Percolator.q.Value":0.00048,"Percolator.PEP":0.0002,"Percolator.SVMScore":1.384,"Precursor.Area":233891.125,"Apex.RT..min.":16.92,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (K4)","Search.ID":"K","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"LEAGLSDSK","Annotated.Sequence":"[K].LEAGLsDSK.[Q]","Modifications":"S6(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.0239,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":500.22415,"Contaminant":"False","Aligned.m.z..Da.":500.22422,"MH...Da.":999.44103,"Theo..MH...Da.":999.43943,"DeltaM..ppm.":1.6,"Deltam.z..Da.":0.0008,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1810606.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":25.94484,"Ion.Inject.Time..ms.":22,"RT..min.":7.5786,"First.Scan":5343,"Last.Scan":5343,"Master.Scan.s.":5334,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_02.raw","File.ID":"F26","Quan.Info":"","Peptides.Matched":748,"XCorr":2.51,"X..Protein.Groups":1,"Percolator.q.Value":0.00048,"Percolator.PEP":0.00017,"Percolator.SVMScore":1.417,"Precursor.Area":3420903.25,"Apex.RT..min.":7.59,"ptmRS..Binomial.Peptide.Score":264.97,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(8): 788.38; y1+-H(3) O(4) P(7): 659.34; y1+-H(3) O(4) P(6): 588.30; y1+-H(3) O(4) P(5): 531.28; y1+-H(3) O(4) P(4): 418.19; y2+-H(3) O(4) P(6): 294.65; y1+(3): 349.17; y1+(2): 234.14","ptmRS..Best.Site.Probabilities":"S6(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(6): 100.0; S(8): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (K4)","Search.ID":"K","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"GVFGYGFEEPSAIQQR","Annotated.Sequence":"[R].GVFGYGFEEPSAIQQR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.5659,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":595.62512,"Contaminant":"False","Aligned.m.z..Da.":595.6251,"MH...Da.":1784.86081,"Theo..MH...Da.":1784.86022,"DeltaM..ppm.":0.33,"Deltam.z..Da.":0.0002,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":418026.90625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":5.41676,"Ion.Inject.Time..ms.":22,"RT..min.":15.0296,"First.Scan":14862,"Last.Scan":14862,"Master.Scan.s.":14850,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_02.raw","File.ID":"F26","Quan.Info":"","Peptides.Matched":1404,"XCorr":2.05,"X..Protein.Groups":1,"Percolator.q.Value":0.00048,"Percolator.PEP":0.00057,"Percolator.SVMScore":1.185,"Precursor.Area":336087.375,"Apex.RT..min.":15.03,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (K4)","Search.ID":"K","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DIFSNDELLSDAYDAK","Annotated.Sequence":"[K].DIFSNDELLSDAYDAK.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.6587,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":605.94832,"Contaminant":"False","Aligned.m.z..Da.":605.94814,"MH...Da.":1815.83042,"Theo..MH...Da.":1815.82831,"DeltaM..ppm.":1.16,"Deltam.z..Da.":0.0007,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":737933.375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":11.08187,"Ion.Inject.Time..ms.":22,"RT..min.":17.0819,"First.Scan":17464,"Last.Scan":17464,"Master.Scan.s.":17460,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_02.raw","File.ID":"F26","Quan.Info":"","Peptides.Matched":1694,"XCorr":2.93,"X..Protein.Groups":1,"Percolator.q.Value":0.00048,"Percolator.PEP":0.00003,"Percolator.SVMScore":1.784,"Precursor.Area":681063.5,"Apex.RT..min.":17.09,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (K4)","Search.ID":"K","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"IQDPQMTGYVSTR","Annotated.Sequence":"[R].IQDPQmtGYVsTR.[Y]","Modifications":"M6(Oxidation); T7(Phospho); S11(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P32485","Master.Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","Protein.Accessions":"P32485","Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.0545,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":836.32852,"Contaminant":"False","Aligned.m.z..Da.":836.32865,"MH...Da.":1671.64976,"Theo..MH...Da.":1671.64853,"DeltaM..ppm.":0.74,"Deltam.z..Da.":0.00062,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2981277,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":10.67825,"Ion.Inject.Time..ms.":22,"RT..min.":7.7456,"First.Scan":5588,"Last.Scan":5588,"Master.Scan.s.":5581,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_02.raw","File.ID":"F26","Quan.Info":"","Peptides.Matched":738,"XCorr":2.75,"X..Protein.Groups":1,"Percolator.q.Value":0.00048,"Percolator.PEP":0.00003,"Percolator.SVMScore":1.746,"Precursor.Area":2866824.5,"Apex.RT..min.":7.75,"ptmRS..Binomial.Peptide.Score":338.57,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(11): 1332.53; y1+-H(3) O(4) P(10): 1217.50; y1+-H(3) O(4) P(8): 992.39; y1+-H(3) O(4) P(7): 845.36; y2+-H(3) O(4) P(11): 666.77; y2+-H(3) O(4) P(10): 609.25; y1+(4): 462.27; y1+(3): 363.20","ptmRS..Best.Site.Probabilities":"T7(Phospho): 100; Y9(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"T(7): 100.0; Y(9): 100.0; S(11): 0.0; T(12): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (K4)","Search.ID":"K","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSSPLHRLSPTGK","Annotated.Sequence":"[R].mSsPLHRLsPTGK.[S]","Modifications":"M1(Oxidation); S3(Phospho); S9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.4684,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":793.84434,"Contaminant":"False","Aligned.m.z..Da.":793.84447,"MH...Da.":1586.68141,"Theo..MH...Da.":1586.67977,"DeltaM..ppm.":1.03,"Deltam.z..Da.":0.00082,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2810234.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":11.71796,"Ion.Inject.Time..ms.":22,"RT..min.":7.7428,"First.Scan":5583,"Last.Scan":5583,"Master.Scan.s.":5581,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_02.raw","File.ID":"F26","Quan.Info":"","Peptides.Matched":1161,"XCorr":2.37,"X..Protein.Groups":1,"Percolator.q.Value":0.00048,"Percolator.PEP":0.00007,"Percolator.SVMScore":1.593,"Precursor.Area":2393506.5,"Apex.RT..min.":7.74,"ptmRS..Binomial.Peptide.Score":286.44,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(3): 304.10; y2+-H(3) O(4) P(11): 627.82; y2+- -2H(3) O(4) P -2H(3) O(4) P(11): 578.83; b1+(2): 235.07","ptmRS..Best.Site.Probabilities":"S3(Phospho): 100; S9(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(3): 100.0; S(9): 100.0; T(11): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (K4)","Search.ID":"K","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"IQDPQMTGYVSTR","Annotated.Sequence":"[R].IQDPQMtGYVsTR.[Y]","Modifications":"T7(Phospho); S11(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P32485","Master.Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","Protein.Accessions":"P32485","Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.0369,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":828.32999,"Contaminant":"False","Aligned.m.z..Da.":828.32995,"MH...Da.":1655.65271,"Theo..MH...Da.":1655.65362,"DeltaM..ppm.":-0.55,"Deltam.z..Da.":-0.00045,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2872823.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":59.54723,"Ion.Inject.Time..ms.":22,"RT..min.":9.8966,"First.Scan":8186,"Last.Scan":8186,"Master.Scan.s.":8181,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_02.raw","File.ID":"F26","Quan.Info":"","Peptides.Matched":740,"XCorr":2.17,"X..Protein.Groups":1,"Percolator.q.Value":0.00048,"Percolator.PEP":0.00111,"Percolator.SVMScore":1.059,"ptmRS..Binomial.Peptide.Score":249.19,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(11): 1316.53; y1+-H(3) O(4) P(10): 1201.51; y1+-H(3) O(4) P(8): 976.40; y1+-H(3) O(4) P(7): 845.36; y2+-H(3) O(4) P(10): 601.26; y1+(4): 462.27; y1+(3): 363.20","ptmRS..Best.Site.Probabilities":"T7(Phospho): 100; Y9(Phospho): 99.42","ptmRS..Phospho.Site.Probabilities":"T(7): 100.0; Y(9): 99.4; S(11): 0.0; T(12): 0.6"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (K4)","Search.ID":"K","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LQQTAFDKK","Annotated.Sequence":"[R].LQQTAFDKK.[S]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.3846,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":360.20151,"Contaminant":"False","Aligned.m.z..Da.":360.20152,"MH...Da.":1078.58999,"Theo..MH...Da.":1078.58914,"DeltaM..ppm.":0.8,"Deltam.z..Da.":0.00029,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3747351.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":27.9182,"Ion.Inject.Time..ms.":20.424,"RT..min.":5.4861,"First.Scan":2249,"Last.Scan":2249,"Master.Scan.s.":2240,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_02.raw","File.ID":"F26","Quan.Info":"","Peptides.Matched":392,"XCorr":2.34,"X..Protein.Groups":1,"Percolator.q.Value":0.00048,"Percolator.PEP":0.00147,"Percolator.SVMScore":1.004,"Precursor.Area":3208748.5,"Apex.RT..min.":5.49,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (K4)","Search.ID":"K","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"TAVFYGGTPISK","Annotated.Sequence":"[K].TAVFYGGTPISK.[D]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.4792,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":620.83028,"Contaminant":"False","Aligned.m.z..Da.":620.83027,"MH...Da.":1240.65328,"Theo..MH...Da.":1240.65721,"DeltaM..ppm.":-3.17,"Deltam.z..Da.":-0.00197,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":622222.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":80.91991,"Ion.Inject.Time..ms.":22,"RT..min.":11.1253,"First.Scan":9735,"Last.Scan":9735,"Master.Scan.s.":9728,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_02.raw","File.ID":"F26","Quan.Info":"","Peptides.Matched":414,"XCorr":2.65,"X..Protein.Groups":1,"Percolator.q.Value":0.00048,"Percolator.PEP":0.00036,"Percolator.SVMScore":1.277,"Precursor.Area":738898.625,"Apex.RT..min.":11.13,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (K4)","Search.ID":"K","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KFQDDTLNR","Annotated.Sequence":"[K].KFQDDtLNR.[V]","Modifications":"T6(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6818,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":608.77265,"Contaminant":"False","Aligned.m.z..Da.":608.77262,"MH...Da.":1216.53803,"Theo..MH...Da.":1216.53579,"DeltaM..ppm.":1.84,"Deltam.z..Da.":0.00112,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1675942.375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":24.8261,"Ion.Inject.Time..ms.":22,"RT..min.":6.2269,"First.Scan":3174,"Last.Scan":3174,"Master.Scan.s.":3163,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_02.raw","File.ID":"F26","Quan.Info":"","Peptides.Matched":657,"XCorr":2.86,"X..Protein.Groups":1,"Percolator.q.Value":0.00048,"Percolator.PEP":0.00009,"Percolator.SVMScore":1.538,"Precursor.Area":2923273.75,"Apex.RT..min.":6.24,"ptmRS..Binomial.Peptide.Score":341.89,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"T6(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"T(6): 100.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (K4)","Search.ID":"K","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEySDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); Y12(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":2,"DeltaScore":0.2157,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":2,"m.z..Da.":967.407,"Contaminant":"False","Aligned.m.z..Da.":967.40676,"MH...Da.":2900.20646,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":2.46,"Deltam.z..Da.":0.00238,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":264092.90625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":86.13486,"Ion.Inject.Time..ms.":22,"RT..min.":16.8192,"First.Scan":17129,"Last.Scan":17129,"Master.Scan.s.":17122,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_02.raw","File.ID":"F26","Quan.Info":"","Peptides.Matched":2591,"XCorr":1.53,"X..Protein.Groups":1,"Percolator.q.Value":0.00048,"Percolator.PEP":0.00006,"Percolator.SVMScore":1.637,"ptmRS..Binomial.Peptide.Score":207.51,"ptmRS..Isoform.Confidence.Probability":0.5,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"Y12(Phospho): 50; S13(Phospho): 50","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 50.0; S(13): 50.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (I4)","Search.ID":"I","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KDELTLEGIK","Annotated.Sequence":"[K].KDELTLEGIK.[Q]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.5502,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":382.5516,"Contaminant":"False","Aligned.m.z..Da.":382.55157,"MH...Da.":1145.64026,"Theo..MH...Da.":1145.64123,"DeltaM..ppm.":-0.85,"Deltam.z..Da.":-0.00032,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3429909,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":37.88658,"Ion.Inject.Time..ms.":22,"RT..min.":10.2552,"First.Scan":8658,"Last.Scan":8658,"Master.Scan.s.":8654,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_04.raw","File.ID":"F28","Quan.Info":"","Peptides.Matched":343,"XCorr":2.69,"X..Protein.Groups":1,"Percolator.q.Value":0.00048,"Percolator.PEP":0.00086,"Percolator.SVMScore":1.2,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (I4)","Search.ID":"I","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MGSINNNFNTNNNSNTDLDR","Annotated.Sequence":"[-].mGSINNNFNTNNNsNTDLDR.[D]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S14(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P39517","Master.Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","Protein.Accessions":"P39517","Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0826,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":749.30966,"Contaminant":"False","Aligned.m.z..Da.":749.30964,"MH...Da.":2245.91443,"Theo..MH...Da.":2245.91057,"DeltaM..ppm.":1.72,"Deltam.z..Da.":0.00129,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":962255.8125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":31.93841,"Ion.Inject.Time..ms.":22,"RT..min.":11.9947,"First.Scan":10991,"Last.Scan":10991,"Master.Scan.s.":10981,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_04.raw","File.ID":"F28","Quan.Info":"","Peptides.Matched":1451,"XCorr":2.18,"X..Protein.Groups":1,"Percolator.q.Value":0.00048,"Percolator.PEP":0.00128,"Percolator.SVMScore":1.042,"ptmRS..Binomial.Peptide.Score":223.94,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(12): 1359.59; y1+-H(3) O(4) P(9): 1030.45; y1+-H(3) O(4) P(8): 916.41; y1+-H(3) O(4) P(7): 802.37; y1+(6): 733.35; y1+(5): 619.30","ptmRS..Best.Site.Probabilities":"S14(Phospho): 99.99","ptmRS..Phospho.Site.Probabilities":"S(3): 0.0; T(10): 0.0; S(14): 100.0; T(16): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (I4)","Search.ID":"I","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ELDEIAASLENL","Annotated.Sequence":"[K].ELDEIAAsLENL.[-]","Modifications":"S8(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6815,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":698.81511,"Contaminant":"False","Aligned.m.z..Da.":698.81463,"MH...Da.":1396.62294,"Theo..MH...Da.":1396.62433,"DeltaM..ppm.":-1,"Deltam.z..Da.":-0.0007,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":640979.8125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":9.90313,"Ion.Inject.Time..ms.":22,"RT..min.":18.2369,"First.Scan":18997,"Last.Scan":18997,"Master.Scan.s.":18989,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_04.raw","File.ID":"F28","Quan.Info":"","Peptides.Matched":922,"XCorr":2.7,"X..Protein.Groups":1,"Percolator.q.Value":0.00048,"Percolator.PEP":0.0011,"Percolator.SVMScore":1.098,"Precursor.Area":1488204,"Apex.RT..min.":18.25,"ptmRS..Binomial.Peptide.Score":202.58,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"S8(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(8): 100.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (I4)","Search.ID":"I","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LEAGLSDSK","Annotated.Sequence":"[K].LEAGLsDSK.[Q]","Modifications":"S6(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.061,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":500.22387,"Contaminant":"False","Aligned.m.z..Da.":500.22389,"MH...Da.":999.44046,"Theo..MH...Da.":999.43943,"DeltaM..ppm.":1.03,"Deltam.z..Da.":0.00052,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3749817,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":14.74041,"Ion.Inject.Time..ms.":22,"RT..min.":7.5781,"First.Scan":5394,"Last.Scan":5394,"Master.Scan.s.":5391,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_04.raw","File.ID":"F28","Quan.Info":"","Peptides.Matched":740,"XCorr":2.46,"X..Protein.Groups":1,"Percolator.q.Value":0.00048,"Percolator.PEP":0.00175,"Percolator.SVMScore":0.943,"Precursor.Area":3920280.5,"Apex.RT..min.":7.59,"ptmRS..Binomial.Peptide.Score":246.76,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(8): 788.38; y1+-H(3) O(4) P(7): 659.34; y1+-H(3) O(4) P(6): 588.30; y1+-H(3) O(4) P(5): 531.28; y1+-H(3) O(4) P(4): 418.19; y2+-H(3) O(4) P(6): 294.65; y1+(3): 349.17; y1+(2): 234.14","ptmRS..Best.Site.Probabilities":"S6(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(6): 100.0; S(8): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (I4)","Search.ID":"I","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ELDEIAASLENL","Annotated.Sequence":"[K].ELDEIAASLENL.[-]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5896,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":658.83282,"Contaminant":"False","Aligned.m.z..Da.":658.83231,"MH...Da.":1316.65836,"Theo..MH...Da.":1316.658,"DeltaM..ppm.":0.27,"Deltam.z..Da.":0.00018,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1488032.625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":18.4569,"First.Scan":19278,"Last.Scan":19278,"Master.Scan.s.":19275,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_04.raw","File.ID":"F28","Quan.Info":"","Peptides.Matched":693,"XCorr":2.51,"X..Protein.Groups":1,"Percolator.q.Value":0.00048,"Percolator.PEP":0.00253,"Percolator.SVMScore":0.845,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (I4)","Search.ID":"I","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"NHQLEEEIEK","Annotated.Sequence":"[K].NHQLEEEIEK.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.4286,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":634.8082,"Contaminant":"False","Aligned.m.z..Da.":634.80819,"MH...Da.":1268.60913,"Theo..MH...Da.":1268.61172,"DeltaM..ppm.":-2.05,"Deltam.z..Da.":-0.0013,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":722418.625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":76.74606,"Ion.Inject.Time..ms.":22,"RT..min.":7.2121,"First.Scan":4846,"Last.Scan":4846,"Master.Scan.s.":4845,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_04.raw","File.ID":"F28","Quan.Info":"","Peptides.Matched":1020,"XCorr":1.4,"X..Protein.Groups":1,"Percolator.q.Value":0.00048,"Percolator.PEP":0.00272,"Percolator.SVMScore":0.827,"Precursor.Area":894885.25,"Apex.RT..min.":7.2,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (I4)","Search.ID":"I","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"NHQLEEEIEK","Annotated.Sequence":"[K].NHQLEEEIEK.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.3929,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":634.81126,"Contaminant":"False","Aligned.m.z..Da.":634.81125,"MH...Da.":1268.61524,"Theo..MH...Da.":1268.61172,"DeltaM..ppm.":2.77,"Deltam.z..Da.":0.00176,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":832485.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":76.68924,"Ion.Inject.Time..ms.":22,"RT..min.":7.1887,"First.Scan":4809,"Last.Scan":4809,"Master.Scan.s.":4806,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_04.raw","File.ID":"F28","Quan.Info":"","Peptides.Matched":866,"XCorr":1.4,"X..Protein.Groups":1,"Percolator.q.Value":0.00048,"Percolator.PEP":0.00241,"Percolator.SVMScore":0.856,"Precursor.Area":894885.25,"Apex.RT..min.":7.2,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (I4)","Search.ID":"I","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"GVFGYGFEEPSAIQQR","Annotated.Sequence":"[R].GVFGYGFEEPSAIQQR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.6111,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":595.6244,"Contaminant":"False","Aligned.m.z..Da.":595.62442,"MH...Da.":1784.85867,"Theo..MH...Da.":1784.86022,"DeltaM..ppm.":-0.87,"Deltam.z..Da.":-0.00052,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":385502.8125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":15.0392,"First.Scan":14932,"Last.Scan":14932,"Master.Scan.s.":14920,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_04.raw","File.ID":"F28","Quan.Info":"","Peptides.Matched":1270,"XCorr":2.16,"X..Protein.Groups":1,"Percolator.q.Value":0.00048,"Percolator.PEP":0.00096,"Percolator.SVMScore":1.152,"Precursor.Area":333006.3125,"Apex.RT..min.":15.04,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (I4)","Search.ID":"I","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ILISTDLLAR","Annotated.Sequence":"[R].ILISTDLLAR.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5171,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":557.84539,"Contaminant":"False","Aligned.m.z..Da.":557.8454,"MH...Da.":1114.68351,"Theo..MH...Da.":1114.68304,"DeltaM..ppm.":0.43,"Deltam.z..Da.":0.00024,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2175409.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":22.82216,"Ion.Inject.Time..ms.":22,"RT..min.":15.5853,"First.Scan":15613,"Last.Scan":15613,"Master.Scan.s.":15609,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_04.raw","File.ID":"F28","Quan.Info":"","Peptides.Matched":124,"XCorr":2.63,"X..Protein.Groups":1,"Percolator.q.Value":0.00048,"Percolator.PEP":0.00085,"Percolator.SVMScore":1.205,"Precursor.Area":2701803.75,"Apex.RT..min.":15.6,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (H4)","Search.ID":"H","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].msEGITDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S2(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":3,"DeltaScore":0.1367,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1096.47138,"Contaminant":"False","Aligned.m.z..Da.":1096.47099,"MH...Da.":2191.93549,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":3.49,"Deltam.z..Da.":0.00382,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":237935.20312,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":75.67226,"Ion.Inject.Time..ms.":22,"RT..min.":15.8595,"First.Scan":16623,"Last.Scan":16623,"Master.Scan.s.":16613,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_05.raw","File.ID":"F29","Quan.Info":"","Peptides.Matched":2212,"XCorr":2.56,"X..Protein.Groups":1,"Percolator.q.Value":0.00049,"Percolator.PEP":0.00263,"Percolator.SVMScore":0.821,"ptmRS..Binomial.Peptide.Score":242.85,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(2): 112.04; b1+-H(3) O(4) P(3): 241.08; b1+-H(3) O(4) P(4): 298.10; b1+-H(3) O(4) P(5): 411.19; b1+-H(3) O(4) P(6): 512.24; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35","ptmRS..Best.Site.Probabilities":"S2(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 100.0; T(6): 0.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (H4)","Search.ID":"H","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"SFLTYIK","Annotated.Sequence":"[K].SFLTYIK.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.601,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":436.24973,"Contaminant":"False","Aligned.m.z..Da.":436.24981,"MH...Da.":871.4922,"Theo..MH...Da.":871.49238,"DeltaM..ppm.":-0.21,"Deltam.z..Da.":-0.00009,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1425433.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":4.6401,"Ion.Inject.Time..ms.":22,"RT..min.":13.8417,"First.Scan":14114,"Last.Scan":14114,"Master.Scan.s.":14111,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_05.raw","File.ID":"F29","Quan.Info":"","Peptides.Matched":273,"XCorr":1.93,"X..Protein.Groups":1,"Percolator.q.Value":0.00049,"Percolator.PEP":0.00286,"Percolator.SVMScore":0.804,"Precursor.Area":7927353,"Apex.RT..min.":13.87,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (H4)","Search.ID":"H","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"IQDPQMTGYVSTR","Annotated.Sequence":"[R].IQDPQMtGYVsTR.[Y]","Modifications":"T7(Phospho); S11(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P32485","Master.Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","Protein.Accessions":"P32485","Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.0545,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":828.32903,"Contaminant":"False","Aligned.m.z..Da.":828.32903,"MH...Da.":1655.65079,"Theo..MH...Da.":1655.65362,"DeltaM..ppm.":-1.7,"Deltam.z..Da.":-0.00141,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1985876,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":30.16038,"Ion.Inject.Time..ms.":22,"RT..min.":9.7964,"First.Scan":8696,"Last.Scan":8696,"Master.Scan.s.":8695,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_05.raw","File.ID":"F29","Quan.Info":"","Peptides.Matched":752,"XCorr":2.2,"X..Protein.Groups":1,"Percolator.q.Value":0.00049,"Percolator.PEP":0.00431,"Percolator.SVMScore":0.724,"Precursor.Area":1412093.875,"Apex.RT..min.":9.8,"ptmRS..Binomial.Peptide.Score":242.5,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(11): 1316.53; y1+-H(3) O(4) P(10): 1201.51; y1+-H(3) O(4) P(8): 976.40; y1+-H(3) O(4) P(7): 845.36; y2+-H(3) O(4) P(10): 601.26; y1+(4): 462.27; y1+(3): 363.20","ptmRS..Best.Site.Probabilities":"T7(Phospho): 100; Y9(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"T(7): 100.0; Y(9): 100.0; S(11): 0.0; T(12): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (H4)","Search.ID":"H","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KSDEPSRESTPVR","Annotated.Sequence":"[K].KSDEPsRESTPVR.[S]","Modifications":"S6(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q08421","Master.Protein.Descriptions":"Enhancer of translation termination 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ETT1 PE=1 SV=1","Protein.Accessions":"Q08421","Protein.Descriptions":"Enhancer of translation termination 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ETT1 PE=1 SV=1","X..Missed.Cleavages":2,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.1191,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":523.24165,"Contaminant":"False","Aligned.m.z..Da.":523.24158,"MH...Da.":1567.71042,"Theo..MH...Da.":1567.71119,"DeltaM..ppm.":-0.49,"Deltam.z..Da.":-0.00026,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":7023863.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":11.61731,"Ion.Inject.Time..ms.":15.2,"RT..min.":4.6746,"First.Scan":1531,"Last.Scan":1531,"Master.Scan.s.":1519,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_05.raw","File.ID":"F29","Quan.Info":"","Peptides.Matched":1087,"XCorr":2.35,"X..Protein.Groups":1,"Percolator.q.Value":0.00049,"Percolator.PEP":0.00351,"Percolator.SVMScore":0.763,"Precursor.Area":3927692.75,"Apex.RT..min.":4.67,"ptmRS..Binomial.Peptide.Score":158.44,"ptmRS..Isoform.Confidence.Probability":0.333,"ptmRS..Isoform.Group.Report":"y2+-H(3) O(4) P(12): 671.32; y2+-H(3) O(4) P(11): 627.81; y2+-H(3) O(4) P(10): 570.29; y2+-H(3) O(4) P(9): 505.77; y2+(7): 422.74","ptmRS..Best.Site.Probabilities":"S6(Phospho): 33.33; S9(Phospho): 33.33; T10(Phospho): 33.33","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(6): 33.3; S(9): 33.3; T(10): 33.3"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Z4)","Search.ID":"Z","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KVIGSFK","Annotated.Sequence":"[K].KVIGSFK.[D]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.2935,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":389.74446,"Contaminant":"False","Aligned.m.z..Da.":389.74448,"MH...Da.":778.48164,"Theo..MH...Da.":778.48215,"DeltaM..ppm.":-0.65,"Deltam.z..Da.":-0.00025,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1390917.375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":51.00381,"Ion.Inject.Time..ms.":22,"RT..min.":6.1964,"First.Scan":3193,"Last.Scan":3193,"Master.Scan.s.":3181,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_05.raw","File.ID":"F11","Quan.Info":"","Peptides.Matched":22,"XCorr":1.84,"X..Protein.Groups":1,"Percolator.q.Value":0.00049,"Percolator.PEP":0.0071,"Percolator.SVMScore":0.575,"Precursor.Area":908955.75,"Apex.RT..min.":6.19,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Z4)","Search.ID":"Z","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ELDEIAASLENL","Annotated.Sequence":"[K].ELDEIAAsLENL.[-]","Modifications":"S8(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.715,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":698.81622,"Contaminant":"False","Aligned.m.z..Da.":698.81662,"MH...Da.":1396.62518,"Theo..MH...Da.":1396.62433,"DeltaM..ppm.":0.6,"Deltam.z..Da.":0.00042,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":809280.5625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":5.86738,"Ion.Inject.Time..ms.":22,"RT..min.":18.2446,"First.Scan":18662,"Last.Scan":18662,"Master.Scan.s.":18659,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_05.raw","File.ID":"F11","Quan.Info":"","Peptides.Matched":850,"XCorr":2.14,"X..Protein.Groups":1,"Percolator.q.Value":0.00049,"Percolator.PEP":0.00407,"Percolator.SVMScore":0.672,"Precursor.Area":608096.125,"Apex.RT..min.":18.25,"ptmRS..Binomial.Peptide.Score":187.65,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"S8(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(8): 100.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (J4)","Search.ID":"J","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ELALQIQK","Annotated.Sequence":"[R].ELALQIQK.[V]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.2066,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":471.78482,"Contaminant":"False","Aligned.m.z..Da.":471.78484,"MH...Da.":942.56237,"Theo..MH...Da.":942.56186,"DeltaM..ppm.":0.54,"Deltam.z..Da.":0.00025,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2885456.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":34.61822,"Ion.Inject.Time..ms.":22,"RT..min.":10.6067,"First.Scan":9065,"Last.Scan":9065,"Master.Scan.s.":9054,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_03.raw","File.ID":"F27","Quan.Info":"","Peptides.Matched":292,"XCorr":2.13,"X..Protein.Groups":1,"Percolator.q.Value":0.0005,"Percolator.PEP":0.00134,"Percolator.SVMScore":1.043,"Precursor.Area":4074702.25,"Apex.RT..min.":10.58,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (J4)","Search.ID":"J","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KDELTLEGIK","Annotated.Sequence":"[K].KDELTLEGIK.[Q]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.5535,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":382.5518,"Contaminant":"False","Aligned.m.z..Da.":382.55181,"MH...Da.":1145.64086,"Theo..MH...Da.":1145.64123,"DeltaM..ppm.":-0.33,"Deltam.z..Da.":-0.00012,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2687060.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":46.29068,"Ion.Inject.Time..ms.":22,"RT..min.":10.2604,"First.Scan":8617,"Last.Scan":8617,"Master.Scan.s.":8612,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_03.raw","File.ID":"F27","Quan.Info":"","Peptides.Matched":314,"XCorr":2.71,"X..Protein.Groups":1,"Percolator.q.Value":0.0005,"Percolator.PEP":0.00093,"Percolator.SVMScore":1.157,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (J4)","Search.ID":"J","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"IQDPQMTGYVSTR","Annotated.Sequence":"[R].IQDPQmTGyVSTR.[Y]","Modifications":"M6(Oxidation); Y9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P32485","Master.Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","Protein.Accessions":"P32485","Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.0791,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":796.3444,"Contaminant":"False","Aligned.m.z..Da.":796.34438,"MH...Da.":1591.68152,"Theo..MH...Da.":1591.6822,"DeltaM..ppm.":-0.42,"Deltam.z..Da.":-0.00034,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":803541.875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":85.3316,"Ion.Inject.Time..ms.":22,"RT..min.":7.6303,"First.Scan":5429,"Last.Scan":5429,"Master.Scan.s.":5427,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_03.raw","File.ID":"F27","Quan.Info":"","Peptides.Matched":1285,"XCorr":1.77,"X..Protein.Groups":1,"Percolator.q.Value":0.0005,"Percolator.PEP":0.00095,"Percolator.SVMScore":1.152,"Precursor.Area":7203631.5,"Apex.RT..min.":7.68,"ptmRS..Binomial.Peptide.Score":129.31,"ptmRS..Isoform.Confidence.Probability":0.497,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"T7(Phospho): 49.73; Y9(Phospho): 49.73","ptmRS..Phospho.Site.Probabilities":"T(7): 49.7; Y(9): 49.7; S(11): 0.0; T(12): 0.5"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (J4)","Search.ID":"J","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LQQTAFDKK","Annotated.Sequence":"[R].LQQTAFDKK.[S]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.4563,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":360.20153,"Contaminant":"False","Aligned.m.z..Da.":360.20151,"MH...Da.":1078.59005,"Theo..MH...Da.":1078.58914,"DeltaM..ppm.":0.85,"Deltam.z..Da.":0.0003,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3925021.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":23.97096,"Ion.Inject.Time..ms.":20.934,"RT..min.":5.4912,"First.Scan":2264,"Last.Scan":2264,"Master.Scan.s.":2255,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_03.raw","File.ID":"F27","Quan.Info":"","Peptides.Matched":392,"XCorr":2.63,"X..Protein.Groups":1,"Percolator.q.Value":0.0005,"Percolator.PEP":0.00168,"Percolator.SVMScore":0.978,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (J4)","Search.ID":"J","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KSDEPSRESTPVR","Annotated.Sequence":"[K].KSDEPsRESTPVR.[S]","Modifications":"S6(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q08421","Master.Protein.Descriptions":"Enhancer of translation termination 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ETT1 PE=1 SV=1","Protein.Accessions":"Q08421","Protein.Descriptions":"Enhancer of translation termination 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ETT1 PE=1 SV=1","X..Missed.Cleavages":2,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0969,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":523.24235,"Contaminant":"False","Aligned.m.z..Da.":523.24225,"MH...Da.":1567.71249,"Theo..MH...Da.":1567.71119,"DeltaM..ppm.":0.83,"Deltam.z..Da.":0.00043,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":6507517,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":22.38614,"Ion.Inject.Time..ms.":17.247,"RT..min.":4.6577,"First.Scan":1446,"Last.Scan":1446,"Master.Scan.s.":1436,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_03.raw","File.ID":"F27","Quan.Info":"","Peptides.Matched":1130,"XCorr":2.27,"X..Protein.Groups":1,"Percolator.q.Value":0.0005,"Percolator.PEP":0.00156,"Percolator.SVMScore":1,"Precursor.Area":4081585.5,"Apex.RT..min.":4.64,"ptmRS..Binomial.Peptide.Score":199.1,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y2+-H(3) O(4) P(12): 671.32; y2+-H(3) O(4) P(11): 627.81; y2+-H(3) O(4) P(10): 570.29; y2+-H(3) O(4) P(9): 505.77; y1+(6): 688.36; y1+(5): 559.32; y2+(7): 422.74","ptmRS..Best.Site.Probabilities":"S6(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(6): 100.0; S(9): 0.0; T(10): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (W4)","Search.ID":"W","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"SFLTYIK","Annotated.Sequence":"[K].SFLTYIK.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6053,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":436.24957,"Contaminant":"False","Aligned.m.z..Da.":436.24956,"MH...Da.":871.49188,"Theo..MH...Da.":871.49238,"DeltaM..ppm.":-0.58,"Deltam.z..Da.":-0.00025,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1661393.125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":13.6807,"First.Scan":13147,"Last.Scan":13147,"Master.Scan.s.":13144,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_02.raw","File.ID":"F14","Quan.Info":"","Peptides.Matched":273,"XCorr":1.9,"X..Protein.Groups":1,"Percolator.q.Value":0.00051,"Percolator.PEP":0.00378,"Percolator.SVMScore":0.806,"Precursor.Area":4883584,"Apex.RT..min.":13.7,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (N4)","Search.ID":"N","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].mSEGItDIEEsQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss); T6(Phospho); S11(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.0642,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1115.44722,"Contaminant":"False","Aligned.m.z..Da.":1115.4469,"MH...Da.":2229.88717,"Theo..MH...Da.":2229.88361,"DeltaM..ppm.":1.6,"Deltam.z..Da.":0.00178,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":313141.0625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":15.5525,"First.Scan":15898,"Last.Scan":15898,"Master.Scan.s.":15893,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_05.raw","File.ID":"F23","Quan.Info":"","Peptides.Matched":1294,"XCorr":1.09,"X..Protein.Groups":1,"Percolator.q.Value":0.00052,"Percolator.PEP":0.00823,"Percolator.SVMScore":0.617,"ptmRS..Binomial.Peptide.Score":208.73,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(7): 665.22; y2+-H(3) O(4) P(18): 1066.46; y1+(9): 1096.53","ptmRS..Best.Site.Probabilities":"S2(Phospho): 99.99; T6(Phospho): 99.99","ptmRS..Phospho.Site.Probabilities":"S(2): 100.0; T(6): 100.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (N4)","Search.ID":"N","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LQQTAFDKK","Annotated.Sequence":"[R].LQQTAFDKK.[S]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.4286,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":360.20124,"Contaminant":"False","Aligned.m.z..Da.":360.20127,"MH...Da.":1078.58916,"Theo..MH...Da.":1078.58914,"DeltaM..ppm.":0.03,"Deltam.z..Da.":1e-05,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":5122045,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":19.70879,"Ion.Inject.Time..ms.":20.762,"RT..min.":5.5543,"First.Scan":2649,"Last.Scan":2649,"Master.Scan.s.":2642,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_05.raw","File.ID":"F23","Quan.Info":"","Peptides.Matched":392,"XCorr":2.59,"X..Protein.Groups":1,"Percolator.q.Value":0.00052,"Percolator.PEP":0.00502,"Percolator.SVMScore":0.713,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (N4)","Search.ID":"N","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"SFLTYIK","Annotated.Sequence":"[K].SFLTYIK.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6382,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":436.24975,"Contaminant":"False","Aligned.m.z..Da.":436.24975,"MH...Da.":871.49223,"Theo..MH...Da.":871.49238,"DeltaM..ppm.":-0.18,"Deltam.z..Da.":-0.00008,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":847259.8125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":5.60431,"Ion.Inject.Time..ms.":22,"RT..min.":13.8448,"First.Scan":14034,"Last.Scan":14034,"Master.Scan.s.":14030,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_05.raw","File.ID":"F23","Quan.Info":"","Peptides.Matched":273,"XCorr":1.52,"X..Protein.Groups":1,"Percolator.q.Value":0.00052,"Percolator.PEP":0.00889,"Percolator.SVMScore":0.602,"Precursor.Area":5403698,"Apex.RT..min.":13.87,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (G4)","Search.ID":"G","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KFQDDTLNR","Annotated.Sequence":"[K].KFQDDtLNR.[V]","Modifications":"T6(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.3921,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":608.7697,"Contaminant":"False","Aligned.m.z..Da.":608.76965,"MH...Da.":1216.53213,"Theo..MH...Da.":1216.53579,"DeltaM..ppm.":-3.01,"Deltam.z..Da.":-0.00183,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3115636,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":57.52345,"Ion.Inject.Time..ms.":22,"RT..min.":6.2682,"First.Scan":3754,"Last.Scan":3754,"Master.Scan.s.":3751,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_06.raw","File.ID":"F30","Quan.Info":"","Peptides.Matched":578,"XCorr":2.27,"X..Protein.Groups":1,"Percolator.q.Value":0.00052,"Percolator.PEP":0.0023,"Percolator.SVMScore":1.009,"Precursor.Area":3796125.5,"Apex.RT..min.":6.25,"ptmRS..Binomial.Peptide.Score":279.09,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"T6(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"T(6): 100.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (G4)","Search.ID":"G","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LQQTAFDKK","Annotated.Sequence":"[R].LQQTAFDKK.[S]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.4436,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":360.20187,"Contaminant":"False","Aligned.m.z..Da.":360.20182,"MH...Da.":1078.59106,"Theo..MH...Da.":1078.58914,"DeltaM..ppm.":1.79,"Deltam.z..Da.":0.00064,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":4517253,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":22.93859,"Ion.Inject.Time..ms.":15.806,"RT..min.":5.542,"First.Scan":2655,"Last.Scan":2655,"Master.Scan.s.":2646,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_06.raw","File.ID":"F30","Quan.Info":"","Peptides.Matched":383,"XCorr":2.66,"X..Protein.Groups":1,"Percolator.q.Value":0.00052,"Percolator.PEP":0.00231,"Percolator.SVMScore":1.008,"Precursor.Area":3302246.25,"Apex.RT..min.":5.54,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (G4)","Search.ID":"G","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"IQDPQMTGYVSTR","Annotated.Sequence":"[R].IQDPQmtGYVsTR.[Y]","Modifications":"M6(Oxidation); T7(Phospho); S11(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P32485","Master.Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","Protein.Accessions":"P32485","Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.0541,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":836.32658,"Contaminant":"False","Aligned.m.z..Da.":836.32666,"MH...Da.":1671.64589,"Theo..MH...Da.":1671.64853,"DeltaM..ppm.":-1.58,"Deltam.z..Da.":-0.00132,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3535017.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":40.53857,"Ion.Inject.Time..ms.":22,"RT..min.":7.6862,"First.Scan":5863,"Last.Scan":5863,"Master.Scan.s.":5857,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_06.raw","File.ID":"F30","Quan.Info":"","Peptides.Matched":720,"XCorr":2.59,"X..Protein.Groups":1,"Percolator.q.Value":0.00052,"Percolator.PEP":0.00228,"Percolator.SVMScore":1.012,"Precursor.Area":4260925,"Apex.RT..min.":7.7,"ptmRS..Binomial.Peptide.Score":294.28,"ptmRS..Isoform.Confidence.Probability":0.005,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(11): 1332.53; y1+-H(3) O(4) P(10): 1217.50; y1+-H(3) O(4) P(8): 992.39; y1+-H(3) O(4) P(7): 845.36; y2+-H(3) O(4) P(11): 666.77; y2+-H(3) O(4) P(10): 609.25; y1+(4): 462.27; y1+(3): 363.20","ptmRS..Best.Site.Probabilities":"T7(Phospho): 100; Y9(Phospho): 98.95","ptmRS..Phospho.Site.Probabilities":"T(7): 100.0; Y(9): 99.0; S(11): 0.5; T(12): 0.5"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (A4)","Search.ID":"A","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ELALQIQK","Annotated.Sequence":"[R].ELALQIQK.[V]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.2524,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":471.78505,"Contaminant":"False","Aligned.m.z..Da.":471.78505,"MH...Da.":942.56282,"Theo..MH...Da.":942.56186,"DeltaM..ppm.":1.03,"Deltam.z..Da.":0.00048,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1707724.875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":15.7681,"Ion.Inject.Time..ms.":22,"RT..min.":10.6093,"First.Scan":9168,"Last.Scan":9168,"Master.Scan.s.":9159,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_03.raw","File.ID":"F36","Quan.Info":"","Peptides.Matched":292,"XCorr":2.06,"X..Protein.Groups":1,"Percolator.q.Value":0.00054,"Percolator.PEP":0.00265,"Percolator.SVMScore":0.883,"Precursor.Area":1132087.75,"Apex.RT..min.":10.61,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (A4)","Search.ID":"A","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ILSSSFHN","Annotated.Sequence":"[K].ILSSsFHN.[-]","Modifications":"S5(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.0892,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":492.71458,"Contaminant":"False","Aligned.m.z..Da.":492.7146,"MH...Da.":984.42188,"Theo..MH...Da.":984.41864,"DeltaM..ppm.":3.3,"Deltam.z..Da.":0.00162,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3158946.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":40.76827,"Ion.Inject.Time..ms.":21.523,"RT..min.":9.3104,"First.Scan":7539,"Last.Scan":7539,"Master.Scan.s.":7534,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_03.raw","File.ID":"F36","Quan.Info":"","Peptides.Matched":385,"XCorr":2.13,"X..Protein.Groups":1,"Percolator.q.Value":0.00054,"Percolator.PEP":0.00195,"Percolator.SVMScore":0.955,"Precursor.Area":1971677.125,"Apex.RT..min.":9.31,"ptmRS..Binomial.Peptide.Score":196,"ptmRS..Isoform.Confidence.Probability":0.996,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(7): 773.36; y1+-H(3) O(4) P(6): 660.27; y1+-H(3) O(4) P(5): 573.24; y1+-H(3) O(4) P(4): 486.21","ptmRS..Best.Site.Probabilities":"S5(Phospho): 99.56","ptmRS..Phospho.Site.Probabilities":"S(3): 0.0; S(4): 0.4; S(5): 99.6"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (A4)","Search.ID":"A","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FDDMELDENLLR","Annotated.Sequence":"[K].FDDmELDENLLR.[G]","Modifications":"M4(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.2917,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":763.34402,"Contaminant":"False","Aligned.m.z..Da.":763.34423,"MH...Da.":1525.68076,"Theo..MH...Da.":1525.6839,"DeltaM..ppm.":-2.06,"Deltam.z..Da.":-0.00157,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":857516.125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":94.56438,"Ion.Inject.Time..ms.":22,"RT..min.":14.4779,"First.Scan":14182,"Last.Scan":14182,"Master.Scan.s.":14177,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_03.raw","File.ID":"F36","Quan.Info":"","Peptides.Matched":964,"XCorr":1.2,"X..Protein.Groups":1,"Percolator.q.Value":0.00054,"Percolator.PEP":0.00225,"Percolator.SVMScore":0.921,"Precursor.Area":629179.625,"Apex.RT..min.":14.49,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (A4)","Search.ID":"A","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ILISTDLLAR","Annotated.Sequence":"[R].ILISTDLLAR.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5541,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":557.84528,"Contaminant":"False","Aligned.m.z..Da.":557.84522,"MH...Da.":1114.68328,"Theo..MH...Da.":1114.68304,"DeltaM..ppm.":0.22,"Deltam.z..Da.":0.00012,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":587659.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":52.20903,"Ion.Inject.Time..ms.":22,"RT..min.":15.644,"First.Scan":15643,"Last.Scan":15643,"Master.Scan.s.":15633,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_03.raw","File.ID":"F36","Quan.Info":"","Peptides.Matched":124,"XCorr":1.57,"X..Protein.Groups":1,"Percolator.q.Value":0.00054,"Percolator.PEP":0.00188,"Percolator.SVMScore":0.962,"Precursor.Area":394230.53125,"Apex.RT..min.":15.64,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (A4)","Search.ID":"A","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ILISTDLLAR","Annotated.Sequence":"[R].ILISTDLLAR.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.4586,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":557.84528,"Contaminant":"False","Aligned.m.z..Da.":557.84522,"MH...Da.":1114.68327,"Theo..MH...Da.":1114.68304,"DeltaM..ppm.":0.21,"Deltam.z..Da.":0.00012,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":587659.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":52.20903,"Ion.Inject.Time..ms.":22,"RT..min.":15.6446,"First.Scan":15644,"Last.Scan":15644,"Master.Scan.s.":15633,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_03.raw","File.ID":"F36","Quan.Info":"","Peptides.Matched":124,"XCorr":1.33,"X..Protein.Groups":1,"Percolator.q.Value":0.00054,"Percolator.PEP":0.00216,"Percolator.SVMScore":0.931,"Precursor.Area":394230.53125,"Apex.RT..min.":15.64,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (X4)","Search.ID":"X","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LQQTAFDKK","Annotated.Sequence":"[R].LQQTAFDKK.[S]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.3705,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":360.20156,"Contaminant":"False","Aligned.m.z..Da.":360.20159,"MH...Da.":1078.59012,"Theo..MH...Da.":1078.58914,"DeltaM..ppm.":0.91,"Deltam.z..Da.":0.00033,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2377154.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":44.39517,"Ion.Inject.Time..ms.":22,"RT..min.":5.4648,"First.Scan":2203,"Last.Scan":2203,"Master.Scan.s.":2192,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_01.raw","File.ID":"F13","Quan.Info":"","Peptides.Matched":392,"XCorr":2.24,"X..Protein.Groups":1,"Percolator.q.Value":0.00054,"Percolator.PEP":0.00527,"Percolator.SVMScore":0.761,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AB4)","Search.ID":"AB","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"SFLTYIK","Annotated.Sequence":"[K].SFLTYIK.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5615,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":436.24837,"Contaminant":"False","Aligned.m.z..Da.":436.24837,"MH...Da.":871.48947,"Theo..MH...Da.":871.49238,"DeltaM..ppm.":-3.34,"Deltam.z..Da.":-0.00146,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1229797.625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":13.6888,"First.Scan":13162,"Last.Scan":13162,"Master.Scan.s.":13160,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_03.raw","File.ID":"F9","Quan.Info":"","Peptides.Matched":310,"XCorr":1.3,"X..Protein.Groups":1,"Percolator.q.Value":0.00054,"Percolator.PEP":0.00641,"Percolator.SVMScore":0.599,"Precursor.Area":2133221.25,"Apex.RT..min.":13.71,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (M4)","Search.ID":"M","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ILISTDLLAR","Annotated.Sequence":"[R].ILISTDLLAR.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.4673,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":557.84596,"Contaminant":"False","Aligned.m.z..Da.":557.84569,"MH...Da.":1114.68465,"Theo..MH...Da.":1114.68304,"DeltaM..ppm.":1.45,"Deltam.z..Da.":0.00081,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":559016.0625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":14.2247,"Ion.Inject.Time..ms.":22,"RT..min.":15.6776,"First.Scan":16250,"Last.Scan":16250,"Master.Scan.s.":16249,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_06.raw","File.ID":"F24","Quan.Info":"","Peptides.Matched":124,"XCorr":2.14,"X..Protein.Groups":1,"Percolator.q.Value":0.00055,"Percolator.PEP":0.00081,"Percolator.SVMScore":1.057,"Precursor.Area":2825688.25,"Apex.RT..min.":15.64,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (M4)","Search.ID":"M","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KSDEPSRESTPVR","Annotated.Sequence":"[K].KSDEPsRESTPVR.[S]","Modifications":"S6(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q08421","Master.Protein.Descriptions":"Enhancer of translation termination 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ETT1 PE=1 SV=1","Protein.Accessions":"Q08421","Protein.Descriptions":"Enhancer of translation termination 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ETT1 PE=1 SV=1","X..Missed.Cleavages":2,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0856,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":523.24152,"Contaminant":"False","Aligned.m.z..Da.":523.2414,"MH...Da.":1567.71,"Theo..MH...Da.":1567.71119,"DeltaM..ppm.":-0.76,"Deltam.z..Da.":-0.0004,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":4210370.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":18.75113,"Ion.Inject.Time..ms.":22,"RT..min.":4.6133,"First.Scan":1594,"Last.Scan":1594,"Master.Scan.s.":1592,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_06.raw","File.ID":"F24","Quan.Info":"","Peptides.Matched":1053,"XCorr":2.57,"X..Protein.Groups":1,"Percolator.q.Value":0.00055,"Percolator.PEP":0.00113,"Percolator.SVMScore":0.992,"Precursor.Area":3284087,"Apex.RT..min.":4.61,"ptmRS..Binomial.Peptide.Score":175.08,"ptmRS..Isoform.Confidence.Probability":0.333,"ptmRS..Isoform.Group.Report":"y2+-H(3) O(4) P(12): 671.32; y2+-H(3) O(4) P(11): 627.81; y2+-H(3) O(4) P(10): 570.29; y2+-H(3) O(4) P(9): 505.77; y2+(7): 422.74","ptmRS..Best.Site.Probabilities":"S6(Phospho): 33.33; S9(Phospho): 33.33; T10(Phospho): 33.33","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(6): 33.3; S(9): 33.3; T(10): 33.3"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (M4)","Search.ID":"M","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LEAGLSDSK","Annotated.Sequence":"[K].LEAGLsDSK.[Q]","Modifications":"S6(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.0957,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":500.22386,"Contaminant":"False","Aligned.m.z..Da.":500.22389,"MH...Da.":999.44045,"Theo..MH...Da.":999.43943,"DeltaM..ppm.":1.02,"Deltam.z..Da.":0.00051,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2326936.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":7.01888,"Ion.Inject.Time..ms.":22,"RT..min.":7.6859,"First.Scan":5802,"Last.Scan":5802,"Master.Scan.s.":5791,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_06.raw","File.ID":"F24","Quan.Info":"","Peptides.Matched":740,"XCorr":2.3,"X..Protein.Groups":1,"Percolator.q.Value":0.00055,"Percolator.PEP":0.00051,"Percolator.SVMScore":1.147,"Precursor.Area":2493800.25,"Apex.RT..min.":7.69,"ptmRS..Binomial.Peptide.Score":269.15,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(8): 788.38; y1+-H(3) O(4) P(7): 659.34; y1+-H(3) O(4) P(6): 588.30; y1+-H(3) O(4) P(5): 531.28; y1+-H(3) O(4) P(4): 418.19; y1+(3): 349.17; y1+(2): 234.14","ptmRS..Best.Site.Probabilities":"S6(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(6): 100.0; S(8): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (M4)","Search.ID":"M","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"IGNNELLHR","Annotated.Sequence":"[K].IGNNELLHR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q03558","Master.Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","Protein.Accessions":"Q03558","Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.5674,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":355.86491,"Contaminant":"False","Aligned.m.z..Da.":355.86494,"MH...Da.":1065.5802,"Theo..MH...Da.":1065.57997,"DeltaM..ppm.":0.21,"Deltam.z..Da.":0.00008,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1790146,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":16.27525,"Ion.Inject.Time..ms.":22,"RT..min.":7.5899,"First.Scan":5669,"Last.Scan":5669,"Master.Scan.s.":5661,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_06.raw","File.ID":"F24","Quan.Info":"","Peptides.Matched":300,"XCorr":2.15,"X..Protein.Groups":1,"Percolator.q.Value":0.00055,"Percolator.PEP":0.00042,"Percolator.SVMScore":1.185,"Precursor.Area":1336458.25,"Apex.RT..min.":7.59,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (M4)","Search.ID":"M","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KFQDDTLNR","Annotated.Sequence":"[K].KFQDDtLNR.[V]","Modifications":"T6(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6963,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":608.77202,"Contaminant":"False","Aligned.m.z..Da.":608.772,"MH...Da.":1216.53677,"Theo..MH...Da.":1216.53579,"DeltaM..ppm.":0.8,"Deltam.z..Da.":0.00049,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1561750.125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":16.468,"Ion.Inject.Time..ms.":22,"RT..min.":6.2228,"First.Scan":3621,"Last.Scan":3621,"Master.Scan.s.":3620,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_06.raw","File.ID":"F24","Quan.Info":"","Peptides.Matched":615,"XCorr":2.7,"X..Protein.Groups":1,"Percolator.q.Value":0.00055,"Percolator.PEP":0.00055,"Percolator.SVMScore":1.134,"Precursor.Area":2167041.5,"Apex.RT..min.":6.23,"ptmRS..Binomial.Peptide.Score":337.92,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"T6(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"T(6): 100.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (M4)","Search.ID":"M","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"NQQLEDEIEK","Annotated.Sequence":"[K].NQQLEDEIEK.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.475,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":623.30083,"Contaminant":"False","Aligned.m.z..Da.":623.30085,"MH...Da.":1245.59439,"Theo..MH...Da.":1245.59574,"DeltaM..ppm.":-1.08,"Deltam.z..Da.":-0.00067,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2254476.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":65.28455,"Ion.Inject.Time..ms.":22,"RT..min.":8.6038,"First.Scan":7035,"Last.Scan":7035,"Master.Scan.s.":7026,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_06.raw","File.ID":"F24","Quan.Info":"","Peptides.Matched":629,"XCorr":1.6,"X..Protein.Groups":1,"Percolator.q.Value":0.00055,"Percolator.PEP":0.00093,"Percolator.SVMScore":1.031,"Precursor.Area":1321308.875,"Apex.RT..min.":8.6,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (U4)","Search.ID":"U","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"HSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[R].HSDsYsENETNHTNVPISSTGGTNNK.[T]","Modifications":"S4(Phospho); S6(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.1531,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":984.05582,"Contaminant":"False","Aligned.m.z..Da.":984.05599,"MH...Da.":2950.15292,"Theo..MH...Da.":2950.15241,"DeltaM..ppm.":0.18,"Deltam.z..Da.":0.00017,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3245193,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":8.029,"First.Scan":6002,"Last.Scan":6002,"Master.Scan.s.":5998,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_04.raw","File.ID":"F16","Quan.Info":"","Peptides.Matched":1122,"XCorr":6.14,"X..Protein.Groups":1,"Percolator.q.Value":0.00056,"Percolator.PEP":5.242e-10,"Percolator.SVMScore":3.855,"Precursor.Area":2830805.75,"Apex.RT..min.":8.03,"ptmRS..Binomial.Peptide.Score":609.53,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(4): 409.15; b1+-H(3) O(4) P(5): 572.21; b1+-H(3) O(4) P(6): 739.21; b1+-H(3) O(4) P(9): 1111.34; b1+-H(3) O(4) P(11): 1326.43; b1+-H(3) O(4) P(15): 1777.64; b2+-H(3) O(4) P(12): 732.25; b2+-H(3) O(4) P(14): 839.79; b2+-H(3) O(4) P(15): 889.33; y2+-H(3) O(4) P(23): 1257.03; y2+- -2H(3) O(4) P -2H(3) O(4) P(24): 1265.56; b1+(2): 225.10; b1+(3): 340.13","ptmRS..Best.Site.Probabilities":"S4(Phospho): 100; S6(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(4): 100.0; Y(5): 0.0; S(6): 100.0; T(10): 0.0; T(13): 0.0; S(18): 0.0; S(19): 0.0; T(20): 0.0; T(23): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (U4)","Search.ID":"U","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"AAEAGETGAATSATEGDNNNNTAAGDKK","Annotated.Sequence":"[K].AAEAGETGAATsATEGDNNNNTAAGDKK.[G]","Modifications":"S12(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0371,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":906.04974,"Contaminant":"False","Aligned.m.z..Da.":906.04967,"MH...Da.":2716.13467,"Theo..MH...Da.":2716.13298,"DeltaM..ppm.":0.62,"Deltam.z..Da.":0.00056,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2032579.875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":5.59172,"Ion.Inject.Time..ms.":22,"RT..min.":5.0232,"First.Scan":1770,"Last.Scan":1770,"Master.Scan.s.":1760,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_04.raw","File.ID":"F16","Quan.Info":"","Peptides.Matched":2527,"XCorr":6.74,"X..Protein.Groups":1,"Percolator.q.Value":0.00056,"Percolator.PEP":7.168e-17,"Percolator.SVMScore":6.937,"ptmRS..Binomial.Peptide.Score":436.26,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(15): 1300.57; y1+-H(3) O(4) P(17): 1688.75; y2+-H(3) O(4) P(25): 1174.02; y2+-H(3) O(4) P(21): 994.95; y2+-H(3) O(4) P(18): 895.40; y2+-H(3) O(4) P(17): 844.88","ptmRS..Best.Site.Probabilities":"S12(Phospho): 99.98","ptmRS..Phospho.Site.Probabilities":"T(7): 0.0; T(11): 0.0; S(12): 100.0; T(14): 0.0; T(22): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (U4)","Search.ID":"U","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"AAEAGETGAATSATEGDNNNNTAAGDKK","Annotated.Sequence":"[K].AAEAGETGAATSATEGDNNNNTAAGDKK.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.7016,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":879.39407,"Contaminant":"False","Aligned.m.z..Da.":879.39399,"MH...Da.":2636.16767,"Theo..MH...Da.":2636.16665,"DeltaM..ppm.":0.39,"Deltam.z..Da.":0.00034,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":587662.625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":4.9467,"First.Scan":1696,"Last.Scan":1696,"Master.Scan.s.":1695,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_04.raw","File.ID":"F16","Quan.Info":"","Peptides.Matched":3594,"XCorr":2.48,"X..Protein.Groups":1,"Percolator.q.Value":0.00056,"Percolator.PEP":4.978e-07,"Percolator.SVMScore":2.518,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (U4)","Search.ID":"U","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"TGTFSIAALQR","Annotated.Sequence":"[K].TGTFSIAALQR.[I]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7544,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":582.82216,"Contaminant":"False","Aligned.m.z..Da.":582.82222,"MH...Da.":1164.63704,"Theo..MH...Da.":1164.63715,"DeltaM..ppm.":-0.09,"Deltam.z..Da.":-0.00005,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1074491.125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":9.99575,"Ion.Inject.Time..ms.":22,"RT..min.":13.3638,"First.Scan":12789,"Last.Scan":12789,"Master.Scan.s.":12784,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_04.raw","File.ID":"F16","Quan.Info":"","Peptides.Matched":364,"XCorr":3.38,"X..Protein.Groups":1,"Percolator.q.Value":0.00056,"Percolator.PEP":6.437e-06,"Percolator.SVMScore":2.019,"Precursor.Area":1234236.75,"Apex.RT..min.":13.37,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (U4)","Search.ID":"U","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"IQDPQMTGYVSTR","Annotated.Sequence":"[R].IQDPQmtGYVsTR.[Y]","Modifications":"M6(Oxidation); T7(Phospho); S11(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P32485","Master.Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","Protein.Accessions":"P32485","Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.0498,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":836.32892,"Contaminant":"False","Aligned.m.z..Da.":836.32903,"MH...Da.":1671.65056,"Theo..MH...Da.":1671.64853,"DeltaM..ppm.":1.21,"Deltam.z..Da.":0.00101,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1930838.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":21.05928,"Ion.Inject.Time..ms.":22,"RT..min.":7.7545,"First.Scan":5645,"Last.Scan":5645,"Master.Scan.s.":5634,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_04.raw","File.ID":"F16","Quan.Info":"","Peptides.Matched":719,"XCorr":2.81,"X..Protein.Groups":1,"Percolator.q.Value":0.00056,"Percolator.PEP":0.00007,"Percolator.SVMScore":1.545,"Precursor.Area":1638655.25,"Apex.RT..min.":7.75,"ptmRS..Binomial.Peptide.Score":306.93,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(11): 1332.53; y1+-H(3) O(4) P(10): 1217.50; y1+-H(3) O(4) P(8): 992.39; y1+-H(3) O(4) P(7): 845.36; y2+-H(3) O(4) P(11): 666.77; y2+-H(3) O(4) P(10): 609.25; y1+(4): 462.27; y1+(3): 363.20","ptmRS..Best.Site.Probabilities":"T7(Phospho): 100; Y9(Phospho): 99.99","ptmRS..Phospho.Site.Probabilities":"T(7): 100.0; Y(9): 100.0; S(11): 0.0; T(12): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (U4)","Search.ID":"U","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"AIMPIIEGHDVLAQAQSGTGK","Annotated.Sequence":"[R].AImPIIEGHDVLAQAQSGTGK.[T]","Modifications":"M3(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.7131,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":718.04119,"Contaminant":"False","Aligned.m.z..Da.":718.04133,"MH...Da.":2152.10902,"Theo..MH...Da.":2152.10668,"DeltaM..ppm.":1.09,"Deltam.z..Da.":0.00078,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":576809.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":15.59988,"Ion.Inject.Time..ms.":22,"RT..min.":12.7899,"First.Scan":12044,"Last.Scan":12044,"Master.Scan.s.":12043,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_04.raw","File.ID":"F16","Quan.Info":"","Peptides.Matched":967,"XCorr":3.73,"X..Protein.Groups":1,"Percolator.q.Value":0.00056,"Percolator.PEP":1.114e-09,"Percolator.SVMScore":3.708,"Precursor.Area":813516.875,"Apex.RT..min.":12.8,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (U4)","Search.ID":"U","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LQETNPEEVPK","Annotated.Sequence":"[K].LQETNPEEVPK.[F]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5368,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":642.32801,"Contaminant":"False","Aligned.m.z..Da.":642.32808,"MH...Da.":1283.64874,"Theo..MH...Da.":1283.64777,"DeltaM..ppm.":0.76,"Deltam.z..Da.":0.00049,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3355294.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":7.639,"First.Scan":5483,"Last.Scan":5483,"Master.Scan.s.":5478,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_04.raw","File.ID":"F16","Quan.Info":"","Peptides.Matched":547,"XCorr":2.72,"X..Protein.Groups":1,"Percolator.q.Value":0.00056,"Percolator.PEP":0.00002,"Percolator.SVMScore":1.777,"Precursor.Area":4755313,"Apex.RT..min.":7.65,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (U4)","Search.ID":"U","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"HSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[R].HSDSYsENETNHTNVPISSTGGTNNK.[T]","Modifications":"S6(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0051,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":957.3999,"Contaminant":"False","Aligned.m.z..Da.":957.40001,"MH...Da.":2870.18515,"Theo..MH...Da.":2870.18608,"DeltaM..ppm.":-0.32,"Deltam.z..Da.":-0.00031,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1810788,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":7.6396,"First.Scan":5484,"Last.Scan":5484,"Master.Scan.s.":5478,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_04.raw","File.ID":"F16","Quan.Info":"","Peptides.Matched":2458,"XCorr":5.9,"X..Protein.Groups":1,"Percolator.q.Value":0.00056,"Percolator.PEP":1.143e-12,"Percolator.SVMScore":5.05,"Precursor.Area":6733955,"Apex.RT..min.":7.65,"ptmRS..Binomial.Peptide.Score":542.69,"ptmRS..Isoform.Confidence.Probability":0.987,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(9): 1031.37; b1+-H(3) O(4) P(10): 1132.42; b1+-H(3) O(4) P(11): 1246.46; b1+-H(3) O(4) P(12): 1383.52; b1+-H(3) O(4) P(15): 1697.68; b2+-H(3) O(4) P(12): 692.26; b2+-H(3) O(4) P(13): 742.79; b2+-H(3) O(4) P(14): 799.81; b2+-H(3) O(4) P(15): 849.34; y2+-H(3) O(4) P(25): 1318.08; y2+-H(3) O(4) P(23): 1217.05; y2+-H(3) O(4) P(21): 1092.00; b1+(4): 427.16","ptmRS..Best.Site.Probabilities":"S6(Phospho): 98.74","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(4): 0.6; Y(5): 0.0; S(6): 98.7; T(10): 0.6; T(13): 0.0; S(18): 0.0; S(19): 0.0; T(20): 0.0; T(23): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (U4)","Search.ID":"U","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"HSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[R].HSDSySENETNHTNVPISSTGGTNNK.[T]","Modifications":"Y5(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":0,"Charge":4,"Original.Precursor.Charge":4,"DeltaScore":0.0123,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":718.30105,"Contaminant":"False","Aligned.m.z..Da.":718.30113,"MH...Da.":2870.18239,"Theo..MH...Da.":2870.18608,"DeltaM..ppm.":-1.28,"Deltam.z..Da.":-0.00092,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1308548.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":6.00286,"Ion.Inject.Time..ms.":22,"RT..min.":7.6407,"First.Scan":5486,"Last.Scan":5486,"Master.Scan.s.":5478,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_04.raw","File.ID":"F16","Quan.Info":"","Peptides.Matched":2353,"XCorr":4.86,"X..Protein.Groups":1,"Percolator.q.Value":0.00056,"Percolator.PEP":5.651e-08,"Percolator.SVMScore":2.942,"Precursor.Area":4219942.5,"Apex.RT..min.":7.65,"ptmRS..Binomial.Peptide.Score":279.07,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(6): 659.24; b1+-H(3) O(4) P(9): 1031.37; b1+-H(3) O(4) P(11): 1246.46; b2+-H(3) O(4) P(7): 394.65; b2+-H(3) O(4) P(8): 451.67; b2+-H(3) O(4) P(14): 799.81; b2+-H(3) O(4) P(15): 849.34; b2+-H(3) O(4) P(17): 954.41; b1+(4): 427.16","ptmRS..Best.Site.Probabilities":"S6(Phospho): 99.27","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(4): 0.7; Y(5): 0.0; S(6): 99.3; T(10): 0.0; T(13): 0.0; S(18): 0.0; S(19): 0.0; T(20): 0.0; T(23): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (U4)","Search.ID":"U","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSSPLHRLSPTGK","Annotated.Sequence":"[R].mSsPLHRLsPTGK.[S]","Modifications":"M1(Oxidation); S3(Phospho); S9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.4298,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":529.56416,"Contaminant":"False","Aligned.m.z..Da.":529.56423,"MH...Da.":1586.67793,"Theo..MH...Da.":1586.67977,"DeltaM..ppm.":-1.16,"Deltam.z..Da.":-0.00061,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2967966.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":14.28393,"Ion.Inject.Time..ms.":22,"RT..min.":7.7145,"First.Scan":5590,"Last.Scan":5590,"Master.Scan.s.":5582,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_04.raw","File.ID":"F16","Quan.Info":"","Peptides.Matched":971,"XCorr":3.49,"X..Protein.Groups":1,"Percolator.q.Value":0.00056,"Percolator.PEP":0.00013,"Percolator.SVMScore":1.431,"Precursor.Area":3754335,"Apex.RT..min.":7.73,"ptmRS..Binomial.Peptide.Score":243.54,"ptmRS..Isoform.Confidence.Probability":0.993,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(8): 877.50; y2+-H(3) O(4) P(12): 671.34; y2+-H(3) O(4) P(11): 627.82; y2+-H(3) O(4) P(10): 544.32; y2+-H(3) O(4) P(8): 439.25; y1+(4): 402.23","ptmRS..Best.Site.Probabilities":"S3(Phospho): 100; S9(Phospho): 99.27","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(3): 100.0; S(9): 99.3; T(11): 0.7"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (U4)","Search.ID":"U","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MGSINNNFNTNNNSNTDLDRDWK","Annotated.Sequence":"[-].mGSINNNFNTNNNsNTDLDRDWK.[T]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S14(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P39517","Master.Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","Protein.Accessions":"P39517","Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.0492,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1338.06446,"Contaminant":"False","Aligned.m.z..Da.":1338.06455,"MH...Da.":2675.12164,"Theo..MH...Da.":2675.11179,"DeltaM..ppm.":3.68,"Deltam.z..Da.":0.00493,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":258913.26562,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":13.5832,"First.Scan":13069,"Last.Scan":13069,"Master.Scan.s.":13057,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_04.raw","File.ID":"F16","Quan.Info":"","Peptides.Matched":2411,"XCorr":4.27,"X..Protein.Groups":1,"Percolator.q.Value":0.00056,"Percolator.PEP":7.269e-09,"Percolator.SVMScore":3.342,"ptmRS..Binomial.Peptide.Score":599.37,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(21): 2244.95; y1+-H(3) O(4) P(15): 1788.79; y1+-H(3) O(4) P(14): 1674.75; y1+-H(3) O(4) P(13): 1573.70; y1+-H(3) O(4) P(10): 1231.57; b2+-H(3) O(4) P(19): 987.41; y2+-H(3) O(4) P(16): 968.43; y2+-H(3) O(4) P(11): 673.31; y1+(9): 1162.55; y1+(8): 1048.51","ptmRS..Best.Site.Probabilities":"S14(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(3): 0.0; T(10): 0.0; S(14): 100.0; T(16): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (U4)","Search.ID":"U","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].mSEGItDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); T6(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1318,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1096.46794,"Contaminant":"False","Aligned.m.z..Da.":1096.46786,"MH...Da.":2191.92861,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":0.35,"Deltam.z..Da.":0.00038,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":283449.125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":15.5646,"First.Scan":15512,"Last.Scan":15512,"Master.Scan.s.":15506,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_04.raw","File.ID":"F16","Quan.Info":"","Peptides.Matched":1967,"XCorr":4.25,"X..Protein.Groups":1,"Percolator.q.Value":0.00056,"Percolator.PEP":6.643e-10,"Percolator.SVMScore":3.809,"Precursor.Area":261703.01562,"Apex.RT..min.":15.55,"ptmRS..Binomial.Peptide.Score":414.97,"ptmRS..Isoform.Confidence.Probability":0.996,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(6): 512.24; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+-H(3) O(4) P(9): 869.39; b1+(3): 259.09; b1+(4): 316.11; b1+(5): 429.20","ptmRS..Best.Site.Probabilities":"T6(Phospho): 99.61","ptmRS..Phospho.Site.Probabilities":"S(2): 0.4; T(6): 99.6; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (U4)","Search.ID":"U","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].mSEGItDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); T6(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1372,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1096.46904,"Contaminant":"False","Aligned.m.z..Da.":1096.46896,"MH...Da.":2191.93081,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":1.35,"Deltam.z..Da.":0.00148,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":273247.6875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":53.86087,"Ion.Inject.Time..ms.":22,"RT..min.":15.5462,"First.Scan":15490,"Last.Scan":15490,"Master.Scan.s.":15480,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_04.raw","File.ID":"F16","Quan.Info":"","Peptides.Matched":2106,"XCorr":4.52,"X..Protein.Groups":1,"Percolator.q.Value":0.00056,"Percolator.PEP":1.688e-09,"Percolator.SVMScore":3.627,"Precursor.Area":261703.01562,"Apex.RT..min.":15.55,"ptmRS..Binomial.Peptide.Score":446.68,"ptmRS..Isoform.Confidence.Probability":0.997,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(6): 512.24; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+-H(3) O(4) P(9): 869.39; b1+-H(3) O(4) P(10): 998.43; b1+(3): 259.09; b1+(4): 316.11; b1+(5): 429.20","ptmRS..Best.Site.Probabilities":"T6(Phospho): 99.66","ptmRS..Phospho.Site.Probabilities":"S(2): 0.3; T(6): 99.7; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (U4)","Search.ID":"U","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FDDMELDENLLR","Annotated.Sequence":"[K].FDDmELDENLLR.[G]","Modifications":"M4(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6136,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":763.34688,"Contaminant":"False","Aligned.m.z..Da.":763.34684,"MH...Da.":1525.68649,"Theo..MH...Da.":1525.6839,"DeltaM..ppm.":1.7,"Deltam.z..Da.":0.0013,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":451897.46875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":29.45673,"Ion.Inject.Time..ms.":22,"RT..min.":14.5301,"First.Scan":14250,"Last.Scan":14250,"Master.Scan.s.":14247,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_04.raw","File.ID":"F16","Quan.Info":"","Peptides.Matched":1099,"XCorr":3.39,"X..Protein.Groups":1,"Percolator.q.Value":0.00056,"Percolator.PEP":8.276e-07,"Percolator.SVMScore":2.419,"Precursor.Area":736634.5625,"Apex.RT..min.":14.49,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (U4)","Search.ID":"U","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].mSEGItDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); T6(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.2,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":731.31446,"Contaminant":"False","Aligned.m.z..Da.":731.31441,"MH...Da.":2191.92884,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":0.45,"Deltam.z..Da.":0.00033,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":179891.57812,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":5.54056,"Ion.Inject.Time..ms.":22,"RT..min.":15.5351,"First.Scan":15476,"Last.Scan":15476,"Master.Scan.s.":15467,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_04.raw","File.ID":"F16","Quan.Info":"","Peptides.Matched":1984,"XCorr":3.55,"X..Protein.Groups":1,"Percolator.q.Value":0.00056,"Percolator.PEP":3.67e-06,"Percolator.SVMScore":2.128,"Precursor.Area":278495.3125,"Apex.RT..min.":15.55,"ptmRS..Binomial.Peptide.Score":332.54,"ptmRS..Isoform.Confidence.Probability":0.995,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(6): 512.24; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+-H(3) O(4) P(9): 869.39; b1+(3): 259.09; b1+(4): 316.11; b1+(5): 429.20","ptmRS..Best.Site.Probabilities":"T6(Phospho): 99.45","ptmRS..Phospho.Site.Probabilities":"S(2): 0.6; T(6): 99.5; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (U4)","Search.ID":"U","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.4175,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40778,"Contaminant":"False","Aligned.m.z..Da.":967.40771,"MH...Da.":2900.20881,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":3.27,"Deltam.z..Da.":0.00316,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":185305.53125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":14.56981,"Ion.Inject.Time..ms.":22,"RT..min.":15.8804,"First.Scan":15909,"Last.Scan":15909,"Master.Scan.s.":15905,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_04.raw","File.ID":"F16","Quan.Info":"","Peptides.Matched":2617,"XCorr":4,"X..Protein.Groups":1,"Percolator.q.Value":0.00056,"Percolator.PEP":6.445e-07,"Percolator.SVMScore":2.467,"ptmRS..Binomial.Peptide.Score":376.57,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(14): 1621.73; y2+-H(3) O(4) P(14): 811.37","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (U4)","Search.ID":"U","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KDELTLEGIK","Annotated.Sequence":"[K].KDELTLEGIK.[Q]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.4943,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":573.3244,"Contaminant":"False","Aligned.m.z..Da.":573.32444,"MH...Da.":1145.64153,"Theo..MH...Da.":1145.64123,"DeltaM..ppm.":0.26,"Deltam.z..Da.":0.00015,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2456429.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":4.45934,"Ion.Inject.Time..ms.":22,"RT..min.":10.2584,"First.Scan":8599,"Last.Scan":8599,"Master.Scan.s.":8598,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_04.raw","File.ID":"F16","Quan.Info":"","Peptides.Matched":307,"XCorr":3.5,"X..Protein.Groups":1,"Percolator.q.Value":0.00056,"Percolator.PEP":5.535e-07,"Percolator.SVMScore":2.497,"Precursor.Area":1692261.625,"Apex.RT..min.":10.26,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (U4)","Search.ID":"U","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"APQALMLAPTR","Annotated.Sequence":"[K].APQALmLAPTR.[E]","Modifications":"M6(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.722,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":592.82661,"Contaminant":"False","Aligned.m.z..Da.":592.82664,"MH...Da.":1184.64594,"Theo..MH...Da.":1184.6456,"DeltaM..ppm.":0.28,"Deltam.z..Da.":0.00017,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1302949.125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":12.22498,"Ion.Inject.Time..ms.":22,"RT..min.":10.1801,"First.Scan":8505,"Last.Scan":8505,"Master.Scan.s.":8494,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_04.raw","File.ID":"F16","Quan.Info":"","Peptides.Matched":304,"XCorr":3.13,"X..Protein.Groups":1,"Percolator.q.Value":0.00056,"Percolator.PEP":6.258e-06,"Percolator.SVMScore":2.024,"Precursor.Area":1740948.25,"Apex.RT..min.":10.19,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (U4)","Search.ID":"U","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].msEGITDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S2(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":3,"DeltaScore":0.171,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1096.46669,"Contaminant":"False","Aligned.m.z..Da.":1096.46661,"MH...Da.":2191.92611,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":-0.79,"Deltam.z..Da.":-0.00087,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":5312167.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":19.88465,"Ion.Inject.Time..ms.":22,"RT..min.":15.7693,"First.Scan":15768,"Last.Scan":15768,"Master.Scan.s.":15762,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_04.raw","File.ID":"F16","Quan.Info":"","Peptides.Matched":1936,"XCorr":3.86,"X..Protein.Groups":1,"Percolator.q.Value":0.00056,"Percolator.PEP":1.239e-07,"Percolator.SVMScore":2.789,"Precursor.Area":10278448,"Apex.RT..min.":15.79,"ptmRS..Binomial.Peptide.Score":357.34,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(2): 112.04; b1+-H(3) O(4) P(3): 241.08; b1+-H(3) O(4) P(4): 298.10; b1+-H(3) O(4) P(5): 411.19; b1+-H(3) O(4) P(6): 512.24; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+-H(3) O(4) P(9): 869.39; b1+-H(3) O(4) P(10): 998.43","ptmRS..Best.Site.Probabilities":"S2(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 100.0; T(6): 0.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (U4)","Search.ID":"U","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":5,"DeltaScore":0.0774,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40685,"Contaminant":"False","Aligned.m.z..Da.":967.40679,"MH...Da.":2900.20601,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":2.3,"Deltam.z..Da.":0.00223,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3113152.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":67.85107,"Ion.Inject.Time..ms.":22,"RT..min.":15.1831,"First.Scan":15040,"Last.Scan":15040,"Master.Scan.s.":15037,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_04.raw","File.ID":"F16","Quan.Info":"","Peptides.Matched":2567,"XCorr":4.78,"X..Protein.Groups":1,"Percolator.q.Value":0.00056,"Percolator.PEP":1.163e-07,"Percolator.SVMScore":2.801,"Precursor.Area":27532502,"Apex.RT..min.":15.09,"ptmRS..Binomial.Peptide.Score":460.58,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(13): 1413.58; b1+-H(3) O(4) P(14): 1528.61; b1+-H(3) O(4) P(16): 1771.69; y1+-H(3) O(4) P(15): 1750.78; y1+-H(3) O(4) P(14): 1621.73; b2+-H(3) O(4) P(18): 1014.90; y2+-H(3) O(4) P(14): 811.37; b1+(12): 1344.56","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (U4)","Search.ID":"U","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":5,"DeltaScore":0.0795,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40447,"Contaminant":"False","Aligned.m.z..Da.":967.4044,"MH...Da.":2900.19886,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":-0.16,"Deltam.z..Da.":-0.00016,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":7789637.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":3.02534,"Ion.Inject.Time..ms.":22,"RT..min.":15.1199,"First.Scan":14962,"Last.Scan":14962,"Master.Scan.s.":14959,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_04.raw","File.ID":"F16","Quan.Info":"","Peptides.Matched":2488,"XCorr":7.04,"X..Protein.Groups":1,"Percolator.q.Value":0.00056,"Percolator.PEP":9.244e-16,"Percolator.SVMScore":6.438,"Precursor.Area":27532502,"Apex.RT..min.":15.09,"ptmRS..Binomial.Peptide.Score":777.79,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(13): 1413.58; b1+-H(3) O(4) P(14): 1528.61; b1+-H(3) O(4) P(15): 1642.65; b1+-H(3) O(4) P(16): 1771.69; y1+-H(3) O(4) P(15): 1750.78; y1+-H(3) O(4) P(14): 1621.73; y1+-H(3) O(4) P(13): 1458.67; b2+-H(3) O(4) P(16): 886.35; b2+-H(3) O(4) P(18): 1014.90; y2+-H(3) O(4) P(14): 811.37; b1+(12): 1344.56; y1+(13): 1556.65","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (U4)","Search.ID":"U","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FTVSAIYSDLPQQER","Annotated.Sequence":"[K].FTVSAIYSDLPQQER.[D]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6891,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":877.44145,"Contaminant":"False","Aligned.m.z..Da.":877.44139,"MH...Da.":1753.87563,"Theo..MH...Da.":1753.87554,"DeltaM..ppm.":0.05,"Deltam.z..Da.":0.00005,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":401736.53125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":15.0163,"First.Scan":14833,"Last.Scan":14833,"Master.Scan.s.":14829,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_04.raw","File.ID":"F16","Quan.Info":"","Peptides.Matched":1079,"XCorr":3.86,"X..Protein.Groups":1,"Percolator.q.Value":0.00056,"Percolator.PEP":1.266e-08,"Percolator.SVMScore":3.234,"Precursor.Area":555311.0625,"Apex.RT..min.":15.03,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (U4)","Search.ID":"U","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"GVFGYGFEEPSAIQQR","Annotated.Sequence":"[R].GVFGYGFEEPSAIQQR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.689,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":892.93314,"Contaminant":"False","Aligned.m.z..Da.":892.93308,"MH...Da.":1784.85901,"Theo..MH...Da.":1784.86022,"DeltaM..ppm.":-0.68,"Deltam.z..Da.":-0.00061,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":583860.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":27.04214,"Ion.Inject.Time..ms.":22,"RT..min.":15.0156,"First.Scan":14832,"Last.Scan":14832,"Master.Scan.s.":14829,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_04.raw","File.ID":"F16","Quan.Info":"","Peptides.Matched":1283,"XCorr":3.73,"X..Protein.Groups":1,"Percolator.q.Value":0.00056,"Percolator.PEP":4.429e-09,"Percolator.SVMScore":3.439,"Precursor.Area":673374.1875,"Apex.RT..min.":15.03,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (U4)","Search.ID":"U","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].mSEGITDIEEsQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S11(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1086,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1096.46831,"Contaminant":"False","Aligned.m.z..Da.":1096.46823,"MH...Da.":2191.92934,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":0.68,"Deltam.z..Da.":0.00075,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":277080.34375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":15.0103,"First.Scan":14827,"Last.Scan":14827,"Master.Scan.s.":14816,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_04.raw","File.ID":"F16","Quan.Info":"","Peptides.Matched":1955,"XCorr":3.04,"X..Protein.Groups":1,"Percolator.q.Value":0.00056,"Percolator.PEP":4.507e-06,"Percolator.SVMScore":2.088,"ptmRS..Binomial.Peptide.Score":295.79,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(14): 1665.76; y1+-H(3) O(4) P(11): 1336.60; y1+-H(3) O(4) P(10): 1207.56; b1+(6): 530.25; b1+(8): 758.36; y1+(9): 1176.49","ptmRS..Best.Site.Probabilities":"S11(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; T(6): 0.0; S(11): 100.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (U4)","Search.ID":"U","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"GVFGYGFEEPSAIQQR","Annotated.Sequence":"[R].GVFGYGFEEPSAIQQR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7009,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":892.93418,"Contaminant":"False","Aligned.m.z..Da.":892.93412,"MH...Da.":1784.86109,"Theo..MH...Da.":1784.86022,"DeltaM..ppm.":0.48,"Deltam.z..Da.":0.00043,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":847762.1875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":44.37867,"Ion.Inject.Time..ms.":22,"RT..min.":15.0369,"First.Scan":14859,"Last.Scan":14859,"Master.Scan.s.":14855,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_04.raw","File.ID":"F16","Quan.Info":"","Peptides.Matched":1393,"XCorr":3.31,"X..Protein.Groups":1,"Percolator.q.Value":0.00056,"Percolator.PEP":3.591e-08,"Percolator.SVMScore":3.03,"Precursor.Area":673374.1875,"Apex.RT..min.":15.03,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (U4)","Search.ID":"U","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MGSINNNFNTNNNSNTDLDRDWK","Annotated.Sequence":"[-].mGSINNNFNTNNNsNTDLDRDWK.[T]","Modifications":"N-Term(Prot)(Met-loss); S14(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P39517","Master.Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","Protein.Accessions":"P39517","Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0518,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":878.36825,"Contaminant":"False","Aligned.m.z..Da.":878.36841,"MH...Da.":2633.09021,"Theo..MH...Da.":2633.10123,"DeltaM..ppm.":-4.18,"Deltam.z..Da.":-0.00367,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1104605.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":11.312,"First.Scan":9982,"Last.Scan":9982,"Master.Scan.s.":9976,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_04.raw","File.ID":"F16","Quan.Info":"","Peptides.Matched":1901,"XCorr":5.79,"X..Protein.Groups":1,"Percolator.q.Value":0.00056,"Percolator.PEP":1.316e-10,"Percolator.SVMScore":4.124,"Precursor.Area":2885912,"Apex.RT..min.":11.33,"ptmRS..Binomial.Peptide.Score":565.81,"ptmRS..Isoform.Confidence.Probability":0.5,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(14): 1674.75; y1+-H(3) O(4) P(13): 1573.70; y1+-H(3) O(4) P(12): 1459.66; y1+-H(3) O(4) P(11): 1345.61; y1+-H(3) O(4) P(10): 1231.57; y2+-H(3) O(4) P(19): 1139.50; y2+-H(3) O(4) P(18): 1082.48; y2+-H(3) O(4) P(17): 1025.45; y2+-H(3) O(4) P(16): 968.43; y2+-H(3) O(4) P(15): 894.90; y2+-H(3) O(4) P(14): 837.88; y2+-H(3) O(4) P(13): 787.35; y2+-H(3) O(4) P(12): 730.33; y2+-H(3) O(4) P(11): 673.31; y2+-H(3) O(4) P(10): 616.29","ptmRS..Best.Site.Probabilities":"S14(Phospho): 50; T16(Phospho): 50","ptmRS..Phospho.Site.Probabilities":"S(3): 0.0; T(10): 0.0; S(14): 50.0; T(16): 50.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (U4)","Search.ID":"U","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].mSEGITDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7062,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1056.48178,"Contaminant":"False","Aligned.m.z..Da.":1056.48172,"MH...Da.":2111.95628,"Theo..MH...Da.":2111.96151,"DeltaM..ppm.":-2.48,"Deltam.z..Da.":-0.00261,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":562697.9375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":86.72675,"Ion.Inject.Time..ms.":22,"RT..min.":14.6536,"First.Scan":14390,"Last.Scan":14390,"Master.Scan.s.":14383,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_04.raw","File.ID":"F16","Quan.Info":"","Peptides.Matched":2232,"XCorr":3.37,"X..Protein.Groups":1,"Percolator.q.Value":0.00056,"Percolator.PEP":1.851e-09,"Percolator.SVMScore":3.609,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (U4)","Search.ID":"U","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0209,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40441,"Contaminant":"False","Aligned.m.z..Da.":967.40434,"MH...Da.":2900.1987,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":-0.22,"Deltam.z..Da.":-0.00021,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3038754.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":6.85495,"Ion.Inject.Time..ms.":22,"RT..min.":15.3622,"First.Scan":15260,"Last.Scan":15260,"Master.Scan.s.":15259,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_04.raw","File.ID":"F16","Quan.Info":"","Peptides.Matched":2467,"XCorr":4.78,"X..Protein.Groups":1,"Percolator.q.Value":0.00056,"Percolator.PEP":9.809e-09,"Percolator.SVMScore":3.284,"Precursor.Area":27532502,"Apex.RT..min.":15.09,"ptmRS..Binomial.Peptide.Score":382.92,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(14): 1528.61; b1+-H(3) O(4) P(16): 1771.69; y1+-H(3) O(4) P(14): 1621.73; b1+(12): 1344.56","ptmRS..Best.Site.Probabilities":"S13(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 0.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (U4)","Search.ID":"U","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DIFSNDELLSDAYDAK","Annotated.Sequence":"[K].DIFSNDELLSDAYDAK.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7455,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":908.42003,"Contaminant":"False","Aligned.m.z..Da.":908.42007,"MH...Da.":1815.83279,"Theo..MH...Da.":1815.82831,"DeltaM..ppm.":2.46,"Deltam.z..Da.":0.00224,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":609918.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":17.0736,"First.Scan":17401,"Last.Scan":17401,"Master.Scan.s.":17396,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_04.raw","File.ID":"F16","Quan.Info":"","Peptides.Matched":1766,"XCorr":3.89,"X..Protein.Groups":1,"Percolator.q.Value":0.00056,"Percolator.PEP":2.813e-08,"Percolator.SVMScore":3.078,"Precursor.Area":1571846.875,"Apex.RT..min.":17.09,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (U4)","Search.ID":"U","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LQETNPEEVPKFEK","Annotated.Sequence":"[K].LQETNPEEVPKFEK.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.4797,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":563.29113,"Contaminant":"False","Aligned.m.z..Da.":563.29116,"MH...Da.":1687.85884,"Theo..MH...Da.":1687.85374,"DeltaM..ppm.":3.02,"Deltam.z..Da.":0.0017,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1378859.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":53.14677,"Ion.Inject.Time..ms.":22,"RT..min.":9.3199,"First.Scan":7486,"Last.Scan":7486,"Master.Scan.s.":7480,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_04.raw","File.ID":"F16","Quan.Info":"","Peptides.Matched":900,"XCorr":1.23,"X..Protein.Groups":1,"Percolator.q.Value":0.00056,"Percolator.PEP":0.00003,"Percolator.SVMScore":1.704,"Precursor.Area":907975.625,"Apex.RT..min.":9.32,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (U4)","Search.ID":"U","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEySDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); Y12(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.4604,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40359,"Contaminant":"False","Aligned.m.z..Da.":967.4036,"MH...Da.":2900.19622,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":-1.07,"Deltam.z..Da.":-0.00104,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":490773.875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":36.89134,"Ion.Inject.Time..ms.":22,"RT..min.":16.9667,"First.Scan":17266,"Last.Scan":17266,"Master.Scan.s.":17263,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_04.raw","File.ID":"F16","Quan.Info":"","Peptides.Matched":2329,"XCorr":3.41,"X..Protein.Groups":1,"Percolator.q.Value":0.00056,"Percolator.PEP":6.257e-07,"Percolator.SVMScore":2.473,"ptmRS..Binomial.Peptide.Score":280.09,"ptmRS..Isoform.Confidence.Probability":0.5,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"Y12(Phospho): 50; S13(Phospho): 50","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 50.0; S(13): 50.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (U4)","Search.ID":"U","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FDDMELDENLLR","Annotated.Sequence":"[K].FDDMELDENLLR.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.519,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":755.35006,"Contaminant":"False","Aligned.m.z..Da.":755.35001,"MH...Da.":1509.69286,"Theo..MH...Da.":1509.68898,"DeltaM..ppm.":2.57,"Deltam.z..Da.":0.00194,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":388253.0625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":16.0347,"First.Scan":16104,"Last.Scan":16104,"Master.Scan.s.":16099,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_04.raw","File.ID":"F16","Quan.Info":"","Peptides.Matched":1089,"XCorr":3.16,"X..Protein.Groups":1,"Percolator.q.Value":0.00056,"Percolator.PEP":0.00002,"Percolator.SVMScore":1.854,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (U4)","Search.ID":"U","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"APQALMLAPTR","Annotated.Sequence":"[K].APQALMLAPTR.[E]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6978,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":584.82902,"Contaminant":"False","Aligned.m.z..Da.":584.82915,"MH...Da.":1168.65078,"Theo..MH...Da.":1168.65069,"DeltaM..ppm.":0.07,"Deltam.z..Da.":0.00004,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":489453.96875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":71.42389,"Ion.Inject.Time..ms.":22,"RT..min.":11.6264,"First.Scan":10425,"Last.Scan":10425,"Master.Scan.s.":10418,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_04.raw","File.ID":"F16","Quan.Info":"","Peptides.Matched":162,"XCorr":2.68,"X..Protein.Groups":1,"Percolator.q.Value":0.00056,"Percolator.PEP":4.146e-06,"Percolator.SVMScore":2.104,"Precursor.Area":378876.53125,"Apex.RT..min.":11.62,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (U4)","Search.ID":"U","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MFILDEADEMLSSGFK","Annotated.Sequence":"[K].mFILDEADEmLSSGFK.[E]","Modifications":"M1(Oxidation); M10(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6762,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":932.91808,"Contaminant":"False","Aligned.m.z..Da.":932.91806,"MH...Da.":1864.82889,"Theo..MH...Da.":1864.83433,"DeltaM..ppm.":-2.91,"Deltam.z..Da.":-0.00272,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":324769.875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":16.6954,"First.Scan":16928,"Last.Scan":16928,"Master.Scan.s.":16918,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_04.raw","File.ID":"F16","Quan.Info":"","Peptides.Matched":1768,"XCorr":2.44,"X..Protein.Groups":1,"Percolator.q.Value":0.00056,"Percolator.PEP":7.818e-07,"Percolator.SVMScore":2.43,"Precursor.Area":371062.9375,"Apex.RT..min.":16.7,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (O4)","Search.ID":"O","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LQQTAFDKK","Annotated.Sequence":"[R].LQQTAFDKK.[S]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.4231,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":360.2021,"Contaminant":"False","Aligned.m.z..Da.":360.20208,"MH...Da.":1078.59176,"Theo..MH...Da.":1078.58914,"DeltaM..ppm.":2.43,"Deltam.z..Da.":0.00087,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3622887,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":27.41948,"Ion.Inject.Time..ms.":22,"RT..min.":5.4863,"First.Scan":2271,"Last.Scan":2271,"Master.Scan.s.":2266,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_04.raw","File.ID":"F22","Quan.Info":"","Peptides.Matched":389,"XCorr":2.34,"X..Protein.Groups":1,"Percolator.q.Value":0.00056,"Percolator.PEP":0.00582,"Percolator.SVMScore":0.678,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (O4)","Search.ID":"O","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":4,"DeltaScore":0.1151,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40504,"Contaminant":"False","Aligned.m.z..Da.":967.40489,"MH...Da.":2900.20056,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":0.43,"Deltam.z..Da.":0.00041,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":209945.07812,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":65.41245,"Ion.Inject.Time..ms.":22,"RT..min.":16.7104,"First.Scan":16991,"Last.Scan":16991,"Master.Scan.s.":16987,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_04.raw","File.ID":"F22","Quan.Info":"","Peptides.Matched":2487,"XCorr":1.39,"X..Protein.Groups":1,"Percolator.q.Value":0.00056,"Percolator.PEP":0.01026,"Percolator.SVMScore":0.57,"ptmRS..Binomial.Peptide.Score":92.25,"ptmRS..Isoform.Confidence.Probability":0.5,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"Y12(Phospho): 49.98; S13(Phospho): 49.98","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 50.0; S(13): 50.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (O4)","Search.ID":"O","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"SFLTYIK","Annotated.Sequence":"[K].SFLTYIK.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6438,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":436.24946,"Contaminant":"False","Aligned.m.z..Da.":436.24963,"MH...Da.":871.49165,"Theo..MH...Da.":871.49238,"DeltaM..ppm.":-0.84,"Deltam.z..Da.":-0.00037,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":558545.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":3.77572,"Ion.Inject.Time..ms.":22,"RT..min.":13.6598,"First.Scan":13142,"Last.Scan":13142,"Master.Scan.s.":13134,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_04.raw","File.ID":"F22","Quan.Info":"","Peptides.Matched":273,"XCorr":1.46,"X..Protein.Groups":1,"Percolator.q.Value":0.00056,"Percolator.PEP":0.01113,"Percolator.SVMScore":0.555,"Precursor.Area":5956436.5,"Apex.RT..min.":13.69,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (T4)","Search.ID":"T","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"TAVFYGGTPISK","Annotated.Sequence":"[K].TAVFYGGTPISK.[D]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.513,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":620.83155,"Contaminant":"False","Aligned.m.z..Da.":620.83159,"MH...Da.":1240.65584,"Theo..MH...Da.":1240.65721,"DeltaM..ppm.":-1.11,"Deltam.z..Da.":-0.00069,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":190191.07812,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":79.80442,"Ion.Inject.Time..ms.":22,"RT..min.":11.2134,"First.Scan":10549,"Last.Scan":10549,"Master.Scan.s.":10548,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_05.raw","File.ID":"F17","Quan.Info":"","Peptides.Matched":408,"XCorr":1.93,"X..Protein.Groups":1,"Percolator.q.Value":0.00057,"Percolator.PEP":0.00884,"Percolator.SVMScore":0.57,"Precursor.Area":181909.57812,"Apex.RT..min.":11.2,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (P4)","Search.ID":"P","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LQQTAFDKK","Annotated.Sequence":"[R].LQQTAFDKK.[S]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.3548,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":360.20119,"Contaminant":"False","Aligned.m.z..Da.":360.20119,"MH...Da.":1078.58904,"Theo..MH...Da.":1078.58914,"DeltaM..ppm.":-0.09,"Deltam.z..Da.":-0.00003,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2648852.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":56.01244,"Ion.Inject.Time..ms.":15.005,"RT..min.":5.5038,"First.Scan":2226,"Last.Scan":2226,"Master.Scan.s.":2214,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_03.raw","File.ID":"F21","Quan.Info":"","Peptides.Matched":392,"XCorr":1.86,"X..Protein.Groups":1,"Percolator.q.Value":0.00057,"Percolator.PEP":0.0056,"Percolator.SVMScore":0.67,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (R4)","Search.ID":"R","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MGSINNNFNTNNNSNTDLDR","Annotated.Sequence":"[-].mGSINNNFNTNNNsNTDLDR.[D]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S14(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P39517","Master.Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","Protein.Accessions":"P39517","Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0929,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":749.31076,"Contaminant":"False","Aligned.m.z..Da.":749.3108,"MH...Da.":2245.91773,"Theo..MH...Da.":2245.91057,"DeltaM..ppm.":3.19,"Deltam.z..Da.":0.00238,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":532119.875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":47.34069,"Ion.Inject.Time..ms.":22,"RT..min.":11.9972,"First.Scan":10805,"Last.Scan":10805,"Master.Scan.s.":10793,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_01.raw","File.ID":"F19","Quan.Info":"","Peptides.Matched":1470,"XCorr":1.83,"X..Protein.Groups":1,"Percolator.q.Value":0.00058,"Percolator.PEP":0.01112,"Percolator.SVMScore":0.569,"Precursor.Area":573941.5625,"Apex.RT..min.":12.01,"ptmRS..Binomial.Peptide.Score":193.61,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(11): 1245.55; y1+-H(3) O(4) P(10): 1144.50; y1+-H(3) O(4) P(9): 1030.45; y1+-H(3) O(4) P(8): 916.41; y1+-H(3) O(4) P(7): 802.37; b2+-H(3) O(4) P(19): 987.41; y2+-H(3) O(4) P(7): 401.69; y1+(6): 733.35; y1+(5): 619.30","ptmRS..Best.Site.Probabilities":"S14(Phospho): 99.98","ptmRS..Phospho.Site.Probabilities":"S(3): 0.0; T(10): 0.0; S(14): 100.0; T(16): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (R4)","Search.ID":"R","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ELDEIAASLENL","Annotated.Sequence":"[K].ELDEIAASLENL.[-]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":658.833,"Contaminant":"False","Aligned.m.z..Da.":658.83315,"MH...Da.":1316.65872,"Theo..MH...Da.":1316.658,"DeltaM..ppm.":0.54,"Deltam.z..Da.":0.00036,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1068961.625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":12.59931,"Ion.Inject.Time..ms.":22,"RT..min.":18.4379,"First.Scan":18961,"Last.Scan":18961,"Master.Scan.s.":18958,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_01.raw","File.ID":"F19","Quan.Info":"","Peptides.Matched":669,"XCorr":1.98,"X..Protein.Groups":1,"Percolator.q.Value":0.00058,"Percolator.PEP":0.01239,"Percolator.SVMScore":0.548,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (K4)","Search.ID":"K","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ELDEIAASLENL","Annotated.Sequence":"[K].ELDEIAAsLENL.[-]","Modifications":"S8(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.704,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":698.81522,"Contaminant":"False","Aligned.m.z..Da.":698.81498,"MH...Da.":1396.62316,"Theo..MH...Da.":1396.62433,"DeltaM..ppm.":-0.84,"Deltam.z..Da.":-0.00059,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1854341.125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":7.99133,"Ion.Inject.Time..ms.":22,"RT..min.":18.2361,"First.Scan":18958,"Last.Scan":18958,"Master.Scan.s.":18955,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_02.raw","File.ID":"F26","Quan.Info":"","Peptides.Matched":927,"XCorr":2.77,"X..Protein.Groups":1,"Percolator.q.Value":0.00059,"Percolator.PEP":0.00257,"Percolator.SVMScore":0.897,"ptmRS..Binomial.Peptide.Score":226.8,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"S8(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(8): 100.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Q4)","Search.ID":"Q","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ELDEIAASLENL","Annotated.Sequence":"[K].ELDEIAAsLENL.[-]","Modifications":"S8(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7203,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":698.81558,"Contaminant":"False","Aligned.m.z..Da.":698.81567,"MH...Da.":1396.62389,"Theo..MH...Da.":1396.62433,"DeltaM..ppm.":-0.32,"Deltam.z..Da.":-0.00022,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1404059.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":18.2423,"First.Scan":18880,"Last.Scan":18880,"Master.Scan.s.":18875,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_02.raw","File.ID":"F20","Quan.Info":"","Peptides.Matched":944,"XCorr":2.86,"X..Protein.Groups":1,"Percolator.q.Value":0.00059,"Percolator.PEP":0.00786,"Percolator.SVMScore":0.694,"Precursor.Area":1037021.625,"Apex.RT..min.":18.25,"ptmRS..Binomial.Peptide.Score":220.79,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"S8(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(8): 100.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"YDRDTFYK","Annotated.Sequence":"[K].YDRDTFYK.[M]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q03558","Master.Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","Protein.Accessions":"Q03558","Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.5179,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":369.84162,"Contaminant":"False","Aligned.m.z..Da.":369.84162,"MH...Da.":1107.5103,"Theo..MH...Da.":1107.51055,"DeltaM..ppm.":-0.23,"Deltam.z..Da.":-0.00008,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":5710515,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":24.75569,"Ion.Inject.Time..ms.":17.706,"RT..min.":7.3452,"First.Scan":5022,"Last.Scan":5022,"Master.Scan.s.":5020,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":553,"XCorr":1.95,"X..Protein.Groups":1,"Percolator.q.Value":0.0006,"Percolator.PEP":0.0047,"Percolator.SVMScore":0.667,"Precursor.Area":3967077.25,"Apex.RT..min.":7.35,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (J4)","Search.ID":"J","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"SFLTYIK","Annotated.Sequence":"[K].SFLTYIK.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.645,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":436.24957,"Contaminant":"False","Aligned.m.z..Da.":436.2497,"MH...Da.":871.49186,"Theo..MH...Da.":871.49238,"DeltaM..ppm.":-0.6,"Deltam.z..Da.":-0.00026,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1826020.875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":3.31771,"Ion.Inject.Time..ms.":22,"RT..min.":13.6764,"First.Scan":13165,"Last.Scan":13165,"Master.Scan.s.":13162,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_03.raw","File.ID":"F27","Quan.Info":"","Peptides.Matched":273,"XCorr":1.69,"X..Protein.Groups":1,"Percolator.q.Value":0.0006,"Percolator.PEP":0.00314,"Percolator.SVMScore":0.809,"Precursor.Area":8887909,"Apex.RT..min.":13.7,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (J4)","Search.ID":"J","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ILISTDLLAR","Annotated.Sequence":"[R].ILISTDLLAR.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5394,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":557.845,"Contaminant":"False","Aligned.m.z..Da.":557.84496,"MH...Da.":1114.68273,"Theo..MH...Da.":1114.68304,"DeltaM..ppm.":-0.28,"Deltam.z..Da.":-0.00015,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":422299.9375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":41.89493,"Ion.Inject.Time..ms.":22,"RT..min.":15.6456,"First.Scan":15673,"Last.Scan":15673,"Master.Scan.s.":15671,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_03.raw","File.ID":"F27","Quan.Info":"","Peptides.Matched":124,"XCorr":1.65,"X..Protein.Groups":1,"Percolator.q.Value":0.0006,"Percolator.PEP":0.00225,"Percolator.SVMScore":0.898,"Precursor.Area":2437072.75,"Apex.RT..min.":15.6,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (A4)","Search.ID":"A","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":2,"DeltaScore":0.1382,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40711,"Contaminant":"False","Aligned.m.z..Da.":967.40743,"MH...Da.":2900.20678,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":2.57,"Deltam.z..Da.":0.00248,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":705807.9375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":85.02916,"Ion.Inject.Time..ms.":22,"RT..min.":17.5858,"First.Scan":18119,"Last.Scan":18119,"Master.Scan.s.":18116,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_03.raw","File.ID":"F36","Quan.Info":"","Peptides.Matched":2587,"XCorr":1.23,"X..Protein.Groups":1,"Percolator.q.Value":0.00061,"Percolator.PEP":0.00534,"Percolator.SVMScore":0.723,"ptmRS..Binomial.Peptide.Score":103.29,"ptmRS..Isoform.Confidence.Probability":0.5,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"Y12(Phospho): 49.98; S13(Phospho): 49.98","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; Y(12): 50.0; S(13): 50.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (A4)","Search.ID":"A","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KFQDDTLNR","Annotated.Sequence":"[K].KFQDDtLNR.[V]","Modifications":"T6(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.4854,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":406.18311,"Contaminant":"False","Aligned.m.z..Da.":406.18314,"MH...Da.":1216.53478,"Theo..MH...Da.":1216.53579,"DeltaM..ppm.":-0.83,"Deltam.z..Da.":-0.00034,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2890447.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":27.51403,"Ion.Inject.Time..ms.":22,"RT..min.":6.2883,"First.Scan":3667,"Last.Scan":3667,"Master.Scan.s.":3660,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_03.raw","File.ID":"F36","Quan.Info":"","Peptides.Matched":618,"XCorr":2.39,"X..Protein.Groups":1,"Percolator.q.Value":0.00061,"Percolator.PEP":0.01089,"Percolator.SVMScore":0.579,"ptmRS..Binomial.Peptide.Score":197.87,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"T6(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"T(6): 100.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (A4)","Search.ID":"A","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"KSDEPSRESTPVR","Annotated.Sequence":"[K].KSDEPsRESTPVR.[S]","Modifications":"S6(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q08421","Master.Protein.Descriptions":"Enhancer of translation termination 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ETT1 PE=1 SV=1","Protein.Accessions":"Q08421","Protein.Descriptions":"Enhancer of translation termination 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ETT1 PE=1 SV=1","X..Missed.Cleavages":2,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0324,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":523.24222,"Contaminant":"False","Aligned.m.z..Da.":523.24225,"MH...Da.":1567.7121,"Theo..MH...Da.":1567.71119,"DeltaM..ppm.":0.58,"Deltam.z..Da.":0.0003,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3758860.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":30.80183,"Ion.Inject.Time..ms.":22,"RT..min.":4.7308,"First.Scan":1720,"Last.Scan":1720,"Master.Scan.s.":1710,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_03.raw","File.ID":"F36","Quan.Info":"","Peptides.Matched":1137,"XCorr":2.16,"X..Protein.Groups":1,"Percolator.q.Value":0.00061,"Percolator.PEP":0.00294,"Percolator.SVMScore":0.858,"Precursor.Area":4341788,"Apex.RT..min.":4.74,"ptmRS..Binomial.Peptide.Score":138.34,"ptmRS..Isoform.Confidence.Probability":0.333,"ptmRS..Isoform.Group.Report":"y2+-H(3) O(4) P(12): 671.32; y2+-H(3) O(4) P(11): 627.81; y2+-H(3) O(4) P(10): 570.29; y2+-H(3) O(4) P(9): 505.77","ptmRS..Best.Site.Probabilities":"S6(Phospho): 33.33; S9(Phospho): 33.33; T10(Phospho): 33.33","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(6): 33.3; S(9): 33.3; T(10): 33.3"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (A4)","Search.ID":"A","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"IQDPQMTGYVSTR","Annotated.Sequence":"[R].IQDPQmTGyVSTR.[Y]","Modifications":"M6(Oxidation); Y9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P32485","Master.Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","Protein.Accessions":"P32485","Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.0408,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":796.34418,"Contaminant":"False","Aligned.m.z..Da.":796.34428,"MH...Da.":1591.68109,"Theo..MH...Da.":1591.6822,"DeltaM..ppm.":-0.7,"Deltam.z..Da.":-0.00056,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":15702985,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":59.66229,"Ion.Inject.Time..ms.":22,"RT..min.":7.6374,"First.Scan":5464,"Last.Scan":5464,"Master.Scan.s.":5454,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_03.raw","File.ID":"F36","Quan.Info":"","Peptides.Matched":1186,"XCorr":0.98,"X..Protein.Groups":1,"Percolator.q.Value":0.00061,"Percolator.PEP":0.01345,"Percolator.SVMScore":0.54,"Precursor.Area":14432895,"Apex.RT..min.":7.63,"ptmRS..Binomial.Peptide.Score":89.12,"ptmRS..Isoform.Confidence.Probability":0.013,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(2): 258.16; y1+(6): 762.32; b2+(11): 618.78","ptmRS..Best.Site.Probabilities":"T12(Phospho): 97.36","ptmRS..Phospho.Site.Probabilities":"T(7): 1.3; Y(9): 1.3; S(11): 0.0; T(12): 97.4"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (A4)","Search.ID":"A","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"KSDEPSRESTPVR","Annotated.Sequence":"[K].KSDEPSREstPVR.[S]","Modifications":"S9(Phospho); T10(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q08421","Master.Protein.Descriptions":"Enhancer of translation termination 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ETT1 PE=1 SV=1","Protein.Accessions":"Q08421","Protein.Descriptions":"Enhancer of translation termination 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ETT1 PE=1 SV=1","X..Missed.Cleavages":2,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0032,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":549.89765,"Contaminant":"False","Aligned.m.z..Da.":549.89768,"MH...Da.":1647.67841,"Theo..MH...Da.":1647.67752,"DeltaM..ppm.":0.54,"Deltam.z..Da.":0.00029,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":10437492,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":39.75744,"Ion.Inject.Time..ms.":11.098,"RT..min.":4.5436,"First.Scan":1479,"Last.Scan":1479,"Master.Scan.s.":1476,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_03.raw","File.ID":"F36","Quan.Info":"","Peptides.Matched":1030,"XCorr":3.16,"X..Protein.Groups":1,"Percolator.q.Value":0.00061,"Percolator.PEP":0.00683,"Percolator.SVMScore":0.671,"Precursor.Area":63555784,"Apex.RT..min.":4.56,"ptmRS..Binomial.Peptide.Score":198.81,"ptmRS..Isoform.Confidence.Probability":0.333,"ptmRS..Isoform.Group.Report":"y2+-H(3) O(4) P(12): 711.31; y2+-H(3) O(4) P(11): 667.79; y2+-H(3) O(4) P(10): 610.28","ptmRS..Best.Site.Probabilities":"S6(Phospho): 66.67; S9(Phospho): 66.67; T10(Phospho): 66.67","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(6): 66.7; S(9): 66.7; T(10): 66.7"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (A4)","Search.ID":"A","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"SFLTYIK","Annotated.Sequence":"[K].SFLTYIK.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6424,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":436.24978,"Contaminant":"False","Aligned.m.z..Da.":436.24993,"MH...Da.":871.49228,"Theo..MH...Da.":871.49238,"DeltaM..ppm.":-0.12,"Deltam.z..Da.":-0.00005,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1216183.625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":13.756,"First.Scan":13272,"Last.Scan":13272,"Master.Scan.s.":13267,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_03.raw","File.ID":"F36","Quan.Info":"","Peptides.Matched":273,"XCorr":1.65,"X..Protein.Groups":1,"Percolator.q.Value":0.00061,"Percolator.PEP":0.00398,"Percolator.SVMScore":0.789,"Precursor.Area":1001788.375,"Apex.RT..min.":13.76,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (A4)","Search.ID":"A","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"QLSEDSHHLQSNNDNFSK","Annotated.Sequence":"[K].QLSEDSHHLQSNNDNFsK.[K]","Modifications":"S17(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.1582,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":727.30615,"Contaminant":"False","Aligned.m.z..Da.":727.30619,"MH...Da.":2179.90391,"Theo..MH...Da.":2179.90403,"DeltaM..ppm.":-0.06,"Deltam.z..Da.":-0.00004,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2203431.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":59.35293,"Ion.Inject.Time..ms.":22,"RT..min.":6.979,"First.Scan":4587,"Last.Scan":4587,"Master.Scan.s.":4583,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_03.raw","File.ID":"F36","Quan.Info":"","Peptides.Matched":1858,"XCorr":2.97,"X..Protein.Groups":1,"Percolator.q.Value":0.00061,"Percolator.PEP":0.00437,"Percolator.SVMScore":0.768,"ptmRS..Binomial.Peptide.Score":179.22,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(11): 1285.59; y1+-H(3) O(4) P(10): 1148.53; y1+-H(3) O(4) P(9): 1035.45; y1+-H(3) O(4) P(8): 907.39; y1+-H(3) O(4) P(4): 477.25; y1+-H(3) O(4) P(3): 363.20; y2+-H(3) O(4) P(16): 920.90; y2+-H(3) O(4) P(14): 812.86; y2+-H(3) O(4) P(13): 755.35; y2+-H(3) O(4) P(11): 643.30","ptmRS..Best.Site.Probabilities":"S17(Phospho): 99.99","ptmRS..Phospho.Site.Probabilities":"S(3): 0.0; S(6): 0.0; S(11): 0.0; S(17): 100.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (A4)","Search.ID":"A","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSSPLHRLSPTGK","Annotated.Sequence":"[R].mSsPLHRLsPTGK.[S]","Modifications":"M1(Oxidation); S3(Phospho); S9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.4706,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":793.84489,"Contaminant":"False","Aligned.m.z..Da.":793.845,"MH...Da.":1586.68251,"Theo..MH...Da.":1586.67977,"DeltaM..ppm.":1.73,"Deltam.z..Da.":0.00137,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1161365.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":67.34489,"Ion.Inject.Time..ms.":22,"RT..min.":7.6916,"First.Scan":5533,"Last.Scan":5533,"Master.Scan.s.":5532,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_03.raw","File.ID":"F36","Quan.Info":"","Peptides.Matched":1132,"XCorr":0.85,"X..Protein.Groups":1,"Percolator.q.Value":0.00061,"Percolator.PEP":0.00895,"Percolator.SVMScore":0.617,"Precursor.Area":5882731.5,"Apex.RT..min.":7.74,"ptmRS..Binomial.Peptide.Score":257.81,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(10): 1087.64; y1+-H(3) O(4) P(8): 877.50; y2+-H(3) O(4) P(11): 627.82; y2+-H(3) O(4) P(10): 544.32; b1+- -2H(3) O(4) P -2H(3) O(4) P(9): 989.50; y1+(4): 402.23","ptmRS..Best.Site.Probabilities":"S3(Phospho): 100; S9(Phospho): 99.99","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(3): 100.0; S(9): 100.0; T(11): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KSDEPSRESTPVR","Annotated.Sequence":"[K].KSDEPsREsTPVR.[S]","Modifications":"S6(Phospho); S9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q08421","Master.Protein.Descriptions":"Enhancer of translation termination 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ETT1 PE=1 SV=1","Protein.Accessions":"Q08421","Protein.Descriptions":"Enhancer of translation termination 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ETT1 PE=1 SV=1","X..Missed.Cleavages":2,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.0951,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":824.34167,"Contaminant":"False","Aligned.m.z..Da.":824.34171,"MH...Da.":1647.67607,"Theo..MH...Da.":1647.67752,"DeltaM..ppm.":-0.88,"Deltam.z..Da.":-0.00073,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":16746411,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":18.53402,"Ion.Inject.Time..ms.":11.519,"RT..min.":4.5703,"First.Scan":1585,"Last.Scan":1585,"Master.Scan.s.":1580,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":956,"XCorr":2.63,"X..Protein.Groups":1,"Percolator.q.Value":0.00062,"Percolator.PEP":0.00314,"Percolator.SVMScore":0.828,"Precursor.Area":19832178,"Apex.RT..min.":4.58,"ptmRS..Binomial.Peptide.Score":367.87,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(9): 998.45; y1+-H(3) O(4) P(10): 1219.55; y1+-H(3) O(4) P(9): 1090.50; y1+-H(3) O(4) P(4): 454.28; y2+-H(3) O(4) P(10): 610.28; y2+-H(3) O(4) P(9): 545.76; b1+- -2H(3) O(4) P -2H(3) O(4) P(10): 1081.49; b1+- -2H(3) O(4) P -2H(3) O(4) P(12): 1277.61","ptmRS..Best.Site.Probabilities":"S6(Phospho): 100; T10(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(6): 100.0; S(9): 0.0; T(10): 100.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"LREENFSSNTSELGNKK","Annotated.Sequence":"[K].LREENFSSNtSELGNKK.[H]","Modifications":"T10(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":2,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0231,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":678.31803,"Contaminant":"False","Aligned.m.z..Da.":678.31804,"MH...Da.":2032.93953,"Theo..MH...Da.":2032.93354,"DeltaM..ppm.":2.95,"Deltam.z..Da.":0.002,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1061517.375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":25.77998,"Ion.Inject.Time..ms.":22,"RT..min.":7.5693,"First.Scan":5156,"Last.Scan":5156,"Master.Scan.s.":5155,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":1847,"XCorr":2.6,"X..Protein.Groups":1,"Percolator.q.Value":0.00062,"Percolator.PEP":0.00272,"Percolator.SVMScore":0.866,"ptmRS..Binomial.Peptide.Score":311.99,"ptmRS..Isoform.Confidence.Probability":0.994,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(10): 1059.54; y1+-H(3) O(4) P(9): 972.51; y1+-H(3) O(4) P(8): 858.47; y2+-H(3) O(4) P(9): 486.76; y2+-H(3) O(4) P(8): 429.74; y1+(7): 775.43","ptmRS..Best.Site.Probabilities":"T10(Phospho): 99.44","ptmRS..Phospho.Site.Probabilities":"S(7): 0.0; S(8): 0.0; T(10): 99.4; S(11): 0.6"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LEAGLSDSK","Annotated.Sequence":"[K].LEAGLsDSK.[Q]","Modifications":"S6(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.0891,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":500.22324,"Contaminant":"False","Aligned.m.z..Da.":500.22326,"MH...Da.":999.43921,"Theo..MH...Da.":999.43943,"DeltaM..ppm.":-0.23,"Deltam.z..Da.":-0.00011,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":5141853.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":2.3705,"Ion.Inject.Time..ms.":22,"RT..min.":7.5898,"First.Scan":5185,"Last.Scan":5185,"Master.Scan.s.":5181,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":608,"XCorr":2.58,"X..Protein.Groups":1,"Percolator.q.Value":0.00062,"Percolator.PEP":0.00298,"Percolator.SVMScore":0.842,"Precursor.Area":7317507,"Apex.RT..min.":7.6,"ptmRS..Binomial.Peptide.Score":271.35,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(8): 788.38; y1+-H(3) O(4) P(7): 659.34; y1+-H(3) O(4) P(6): 588.30; y1+-H(3) O(4) P(5): 531.28; y1+-H(3) O(4) P(4): 418.19; y1+(3): 349.17; y1+(2): 234.14","ptmRS..Best.Site.Probabilities":"S6(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(6): 100.0; S(8): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DFLLKPELSR","Annotated.Sequence":"[K].DFLLKPELSR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6528,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":609.34837,"Contaminant":"False","Aligned.m.z..Da.":609.34841,"MH...Da.":1217.68948,"Theo..MH...Da.":1217.68885,"DeltaM..ppm.":0.51,"Deltam.z..Da.":0.00031,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":801404,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":9.30429,"Ion.Inject.Time..ms.":22,"RT..min.":13.1686,"First.Scan":12563,"Last.Scan":12563,"Master.Scan.s.":12552,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":265,"XCorr":2.16,"X..Protein.Groups":1,"Percolator.q.Value":0.00062,"Percolator.PEP":0.003,"Percolator.SVMScore":0.84,"Precursor.Area":928786.25,"Apex.RT..min.":13.18,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSSPLHRLSPTGK","Annotated.Sequence":"[R].mSsPLHRLsPTGK.[S]","Modifications":"M1(Oxidation); S3(Phospho); S9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.588,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":793.84321,"Contaminant":"False","Aligned.m.z..Da.":793.84324,"MH...Da.":1586.67914,"Theo..MH...Da.":1586.67977,"DeltaM..ppm.":-0.4,"Deltam.z..Da.":-0.00032,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":5384395,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":28.46555,"Ion.Inject.Time..ms.":22,"RT..min.":7.7145,"First.Scan":5362,"Last.Scan":5362,"Master.Scan.s.":5350,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":974,"XCorr":2.33,"X..Protein.Groups":1,"Percolator.q.Value":0.00062,"Percolator.PEP":0.00288,"Percolator.SVMScore":0.85,"Precursor.Area":4913041,"Apex.RT..min.":7.72,"ptmRS..Binomial.Peptide.Score":282.25,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(8): 877.50; y1+-H(3) O(4) P(5): 471.26; y2+-H(3) O(4) P(11): 627.82; y2+-H(3) O(4) P(10): 544.32; b1+- -2H(3) O(4) P -2H(3) O(4) P(9): 989.50; y1+(4): 402.23; y1+(3): 305.18","ptmRS..Best.Site.Probabilities":"S3(Phospho): 100; S9(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(3): 100.0; S(9): 100.0; T(11): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AC4)","Search.ID":"AC","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"APQALMLAPTR","Annotated.Sequence":"[K].APQALMLAPTR.[E]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":3,"DeltaScore":0.5321,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":584.82972,"Contaminant":"False","Aligned.m.z..Da.":584.82972,"MH...Da.":1168.65216,"Theo..MH...Da.":1168.65069,"DeltaM..ppm.":1.26,"Deltam.z..Da.":0.00073,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":152293.5625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":89.30317,"Ion.Inject.Time..ms.":22,"RT..min.":11.6171,"First.Scan":10373,"Last.Scan":10373,"Master.Scan.s.":10370,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_02.raw","File.ID":"F8","Quan.Info":"","Peptides.Matched":202,"XCorr":1.09,"X..Protein.Groups":1,"Percolator.q.Value":0.00063,"Percolator.PEP":0.00773,"Percolator.SVMScore":0.522,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (V4)","Search.ID":"V","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LQETNPEEVPKFEK","Annotated.Sequence":"[K].LQETNPEEVPKFEK.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.4167,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":563.29009,"Contaminant":"False","Aligned.m.z..Da.":563.2901,"MH...Da.":1687.85573,"Theo..MH...Da.":1687.85374,"DeltaM..ppm.":1.18,"Deltam.z..Da.":0.00066,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1672275.625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":57.56885,"Ion.Inject.Time..ms.":8.307,"RT..min.":9.3111,"First.Scan":7461,"Last.Scan":7461,"Master.Scan.s.":7454,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_03.raw","File.ID":"F15","Quan.Info":"","Peptides.Matched":813,"XCorr":0.96,"X..Protein.Groups":1,"Percolator.q.Value":0.00063,"Percolator.PEP":0.00222,"Percolator.SVMScore":0.779,"Precursor.Area":961960.125,"Apex.RT..min.":9.31,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (V4)","Search.ID":"V","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DFLLKPELSR","Annotated.Sequence":"[K].DFLLKPELSR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.6561,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":406.56783,"Contaminant":"False","Aligned.m.z..Da.":406.56787,"MH...Da.":1217.68896,"Theo..MH...Da.":1217.68885,"DeltaM..ppm.":0.09,"Deltam.z..Da.":0.00004,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":198316.03125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":13.173,"First.Scan":12513,"Last.Scan":12513,"Master.Scan.s.":12511,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_03.raw","File.ID":"F15","Quan.Info":"","Peptides.Matched":265,"XCorr":1.57,"X..Protein.Groups":1,"Percolator.q.Value":0.00063,"Percolator.PEP":0.00244,"Percolator.SVMScore":0.761,"Precursor.Area":214383.79688,"Apex.RT..min.":13.15,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AA4)","Search.ID":"AA","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LQQTAFDKK","Annotated.Sequence":"[R].LQQTAFDKK.[S]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":4,"DeltaScore":0.3906,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":360.20134,"Contaminant":"False","Aligned.m.z..Da.":360.20136,"MH...Da.":1078.58947,"Theo..MH...Da.":1078.58914,"DeltaM..ppm.":0.31,"Deltam.z..Da.":0.00011,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1009630.3125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":80.17556,"Ion.Inject.Time..ms.":18.432,"RT..min.":5.4767,"First.Scan":2255,"Last.Scan":2255,"Master.Scan.s.":2246,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_04.raw","File.ID":"F10","Quan.Info":"","Peptides.Matched":392,"XCorr":1.92,"X..Protein.Groups":1,"Percolator.q.Value":0.00063,"Percolator.PEP":0.0058,"Percolator.SVMScore":0.631,"Precursor.Area":641929.375,"Apex.RT..min.":5.47,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (N4)","Search.ID":"N","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LEAGLSDSK","Annotated.Sequence":"[K].LEAGLsDSK.[Q]","Modifications":"S6(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":3,"DeltaScore":0.0617,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":500.22283,"Contaminant":"False","Aligned.m.z..Da.":500.22298,"MH...Da.":999.43839,"Theo..MH...Da.":999.43943,"DeltaM..ppm.":-1.05,"Deltam.z..Da.":-0.00052,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2191919.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":65.1409,"Ion.Inject.Time..ms.":22,"RT..min.":7.6742,"First.Scan":5888,"Last.Scan":5888,"Master.Scan.s.":5879,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_05.raw","File.ID":"F23","Quan.Info":"","Peptides.Matched":602,"XCorr":2.27,"X..Protein.Groups":1,"Percolator.q.Value":0.00064,"Percolator.PEP":0.01354,"Percolator.SVMScore":0.52,"Precursor.Area":2096116.375,"Apex.RT..min.":7.68,"ptmRS..Binomial.Peptide.Score":172.28,"ptmRS..Isoform.Confidence.Probability":0.995,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(8): 788.38; y1+-H(3) O(4) P(7): 659.34; y1+-H(3) O(4) P(6): 588.30; y1+(3): 349.17; y1+(2): 234.14","ptmRS..Best.Site.Probabilities":"S6(Phospho): 99.49","ptmRS..Phospho.Site.Probabilities":"S(6): 99.5; S(8): 0.5"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (M4)","Search.ID":"M","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LQQTAFDK","Annotated.Sequence":"[R].LQQTAFDK.[K]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.4221,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":475.75131,"Contaminant":"False","Aligned.m.z..Da.":475.75132,"MH...Da.":950.49535,"Theo..MH...Da.":950.49417,"DeltaM..ppm.":1.24,"Deltam.z..Da.":0.00059,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":5836064,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":71.67987,"Ion.Inject.Time..ms.":8.097,"RT..min.":7.1157,"First.Scan":5001,"Last.Scan":5001,"Master.Scan.s.":4998,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_06.raw","File.ID":"F24","Quan.Info":"","Peptides.Matched":479,"XCorr":1.54,"X..Protein.Groups":1,"Percolator.q.Value":0.00065,"Percolator.PEP":0.00281,"Percolator.SVMScore":0.815,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (P4)","Search.ID":"P","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KSDEPSRESTPVR","Annotated.Sequence":"[K].KSDEPSREstPVR.[S]","Modifications":"S9(Phospho); T10(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q08421","Master.Protein.Descriptions":"Enhancer of translation termination 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ETT1 PE=1 SV=1","Protein.Accessions":"Q08421","Protein.Descriptions":"Enhancer of translation termination 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ETT1 PE=1 SV=1","X..Missed.Cleavages":2,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.2569,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":824.34215,"Contaminant":"False","Aligned.m.z..Da.":824.34192,"MH...Da.":1647.67702,"Theo..MH...Da.":1647.67752,"DeltaM..ppm.":-0.31,"Deltam.z..Da.":-0.00025,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":10701763,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":15.38,"RT..min.":4.5611,"First.Scan":1345,"Last.Scan":1345,"Master.Scan.s.":1343,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_03.raw","File.ID":"F21","Quan.Info":"","Peptides.Matched":973,"XCorr":2.53,"X..Protein.Groups":1,"Percolator.q.Value":0.00067,"Percolator.PEP":0.01019,"Percolator.SVMScore":0.559,"Precursor.Area":5322306.5,"Apex.RT..min.":4.55,"ptmRS..Binomial.Peptide.Score":350.37,"ptmRS..Isoform.Confidence.Probability":0.499,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(8): 911.42; b1+-H(3) O(4) P(9): 998.45; b1+-H(3) O(4) P(10): 1179.47; b1+-H(3) O(4) P(12): 1375.59; y2+- -2H(3) O(4) P -2H(3) O(4) P(10): 561.29; y2+- -2H(3) O(4) P -2H(3) O(4) P(9): 496.77","ptmRS..Best.Site.Probabilities":"S6(Phospho): 50.13; S9(Phospho): 50.13; T10(Phospho): 99.73","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(6): 50.1; S(9): 50.1; T(10): 99.7"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (L4)","Search.ID":"L","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"SFLTYIK","Annotated.Sequence":"[K].SFLTYIK.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6748,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":436.24987,"Contaminant":"False","Aligned.m.z..Da.":436.24998,"MH...Da.":871.49246,"Theo..MH...Da.":871.49238,"DeltaM..ppm.":0.09,"Deltam.z..Da.":0.00004,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":897516.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":4.42934,"Ion.Inject.Time..ms.":22,"RT..min.":13.6658,"First.Scan":13025,"Last.Scan":13025,"Master.Scan.s.":13022,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_01.raw","File.ID":"F25","Quan.Info":"","Peptides.Matched":273,"XCorr":1.63,"X..Protein.Groups":1,"Percolator.q.Value":0.00067,"Percolator.PEP":0.00914,"Percolator.SVMScore":0.679,"Precursor.Area":7984562,"Apex.RT..min.":13.7,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSSPLHRLSPTGK","Annotated.Sequence":"[R].mSsPLHRLsPTGK.[S]","Modifications":"M1(Oxidation); S3(Phospho); S9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6164,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":793.84244,"Contaminant":"False","Aligned.m.z..Da.":793.84244,"MH...Da.":1586.67761,"Theo..MH...Da.":1586.67977,"DeltaM..ppm.":-1.36,"Deltam.z..Da.":-0.00108,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3391093.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":51.37976,"Ion.Inject.Time..ms.":22,"RT..min.":7.7454,"First.Scan":5549,"Last.Scan":5549,"Master.Scan.s.":5540,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":976,"XCorr":1.46,"X..Protein.Groups":1,"Percolator.q.Value":0.00068,"Percolator.PEP":0.0066,"Percolator.SVMScore":0.603,"Precursor.Area":5832718.5,"Apex.RT..min.":7.71,"ptmRS..Binomial.Peptide.Score":212.81,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(8): 877.50; y2+-H(3) O(4) P(11): 627.82; y2+-H(3) O(4) P(10): 544.32; b1+- -2H(3) O(4) P -2H(3) O(4) P(9): 989.50; y1+(4): 402.23","ptmRS..Best.Site.Probabilities":"S3(Phospho): 100; S9(Phospho): 99.99","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(3): 100.0; S(9): 100.0; T(11): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"VLEELDMR","Annotated.Sequence":"[K].VLEELDMR.[R]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5769,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":502.75751,"Contaminant":"False","Aligned.m.z..Da.":502.75751,"MH...Da.":1004.50774,"Theo..MH...Da.":1004.50811,"DeltaM..ppm.":-0.36,"Deltam.z..Da.":-0.00018,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3404103.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":10.75662,"Ion.Inject.Time..ms.":22,"RT..min.":11.1478,"First.Scan":10007,"Last.Scan":10007,"Master.Scan.s.":9999,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":500,"XCorr":2.08,"X..Protein.Groups":1,"Percolator.q.Value":0.00068,"Percolator.PEP":0.00616,"Percolator.SVMScore":0.616,"Precursor.Area":2236405.5,"Apex.RT..min.":11.14,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DFLLKPELSR","Annotated.Sequence":"[K].DFLLKPELSR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6459,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":609.3478,"Contaminant":"False","Aligned.m.z..Da.":609.3478,"MH...Da.":1217.68833,"Theo..MH...Da.":1217.68885,"DeltaM..ppm.":-0.42,"Deltam.z..Da.":-0.00026,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1423800.875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":22.8353,"Ion.Inject.Time..ms.":22,"RT..min.":13.1546,"First.Scan":12611,"Last.Scan":12611,"Master.Scan.s.":12599,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":275,"XCorr":2.09,"X..Protein.Groups":1,"Percolator.q.Value":0.00068,"Percolator.PEP":0.00623,"Percolator.SVMScore":0.614,"Precursor.Area":3045295.75,"Apex.RT..min.":13.17,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (G4)","Search.ID":"G","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].mSEGItDIEEsQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss); T6(Phospho); S11(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.0253,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1115.44741,"Contaminant":"False","Aligned.m.z..Da.":1115.44746,"MH...Da.":2229.88754,"Theo..MH...Da.":2229.88361,"DeltaM..ppm.":1.76,"Deltam.z..Da.":0.00197,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":389984.5625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":15.7175,"First.Scan":16453,"Last.Scan":16453,"Master.Scan.s.":16442,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_06.raw","File.ID":"F30","Quan.Info":"","Peptides.Matched":1243,"XCorr":1.58,"X..Protein.Groups":1,"Percolator.q.Value":0.0007,"Percolator.PEP":0.00509,"Percolator.SVMScore":0.798,"ptmRS..Binomial.Peptide.Score":241.52,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(7): 665.22; b1+-H(3) O(4) P(9): 907.34; y2+-H(3) O(4) P(18): 1066.46; y1+(12): 1467.70; y1+(9): 1096.53","ptmRS..Best.Site.Probabilities":"S2(Phospho): 100; T6(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 100.0; T(6): 100.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (G4)","Search.ID":"G","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSSPLHRLSPTGK","Annotated.Sequence":"[R].mSsPLHRLsPTGK.[S]","Modifications":"M1(Oxidation); S3(Phospho); S9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":4,"DeltaScore":0.4793,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":529.56516,"Contaminant":"False","Aligned.m.z..Da.":529.56521,"MH...Da.":1586.68092,"Theo..MH...Da.":1586.67977,"DeltaM..ppm.":0.72,"Deltam.z..Da.":0.00038,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":9673136,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":33.7175,"Ion.Inject.Time..ms.":13.316,"RT..min.":7.7094,"First.Scan":5894,"Last.Scan":5894,"Master.Scan.s.":5883,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_06.raw","File.ID":"F30","Quan.Info":"","Peptides.Matched":1097,"XCorr":2.42,"X..Protein.Groups":1,"Percolator.q.Value":0.0007,"Percolator.PEP":0.00437,"Percolator.SVMScore":0.837,"ptmRS..Binomial.Peptide.Score":119.86,"ptmRS..Isoform.Confidence.Probability":0.971,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(8): 877.50; y2+-H(3) O(4) P(11): 627.82; b2+- -2H(3) O(4) P -2H(3) O(4) P(11): 594.30; b1+(2): 235.07; y2+(10): 593.31","ptmRS..Best.Site.Probabilities":"S3(Phospho): 99.98; S9(Phospho): 97.17","ptmRS..Phospho.Site.Probabilities":"S(2): 1.4; S(3): 100.0; S(9): 97.2; T(11): 1.4"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (H4)","Search.ID":"H","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KVIGSFK","Annotated.Sequence":"[K].KVIGSFK.[D]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.2663,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":389.74459,"Contaminant":"False","Aligned.m.z..Da.":389.7446,"MH...Da.":778.48191,"Theo..MH...Da.":778.48215,"DeltaM..ppm.":-0.31,"Deltam.z..Da.":-0.00012,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1973837.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":68.40885,"Ion.Inject.Time..ms.":22,"RT..min.":6.3244,"First.Scan":4008,"Last.Scan":4008,"Master.Scan.s.":4002,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_05.raw","File.ID":"F29","Quan.Info":"","Peptides.Matched":22,"XCorr":1.99,"X..Protein.Groups":1,"Percolator.q.Value":0.0007,"Percolator.PEP":0.00722,"Percolator.SVMScore":0.632,"Precursor.Area":1673005.625,"Apex.RT..min.":6.33,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (M4)","Search.ID":"M","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FFISNPDLVDR","Annotated.Sequence":"[R].FFISNPDLVDR.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q03558","Master.Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","Protein.Accessions":"Q03558","Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6513,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":661.83984,"Contaminant":"False","Aligned.m.z..Da.":661.83992,"MH...Da.":1322.67241,"Theo..MH...Da.":1322.67393,"DeltaM..ppm.":-1.14,"Deltam.z..Da.":-0.00076,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":717022.5625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":54.75604,"Ion.Inject.Time..ms.":22,"RT..min.":14.716,"First.Scan":15090,"Last.Scan":15090,"Master.Scan.s.":15083,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_06.raw","File.ID":"F24","Quan.Info":"","Peptides.Matched":669,"XCorr":1.95,"X..Protein.Groups":1,"Percolator.q.Value":0.00072,"Percolator.PEP":0.00484,"Percolator.SVMScore":0.71,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (M4)","Search.ID":"M","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LQQTAFDKK","Annotated.Sequence":"[R].LQQTAFDKK.[S]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.4174,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":360.2018,"Contaminant":"False","Aligned.m.z..Da.":360.20177,"MH...Da.":1078.59084,"Theo..MH...Da.":1078.58914,"DeltaM..ppm.":1.58,"Deltam.z..Da.":0.00057,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3102460.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":22.54382,"Ion.Inject.Time..ms.":21.739,"RT..min.":5.5105,"First.Scan":2602,"Last.Scan":2602,"Master.Scan.s.":2593,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_06.raw","File.ID":"F24","Quan.Info":"","Peptides.Matched":382,"XCorr":2.42,"X..Protein.Groups":1,"Percolator.q.Value":0.00072,"Percolator.PEP":0.00515,"Percolator.SVMScore":0.698,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (M4)","Search.ID":"M","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].msEGITDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S2(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":3,"DeltaScore":0.1649,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1096.46833,"Contaminant":"False","Aligned.m.z..Da.":1096.46775,"MH...Da.":2191.92938,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":0.7,"Deltam.z..Da.":0.00077,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":514520.4375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":68.6349,"Ion.Inject.Time..ms.":22,"RT..min.":15.8495,"First.Scan":16452,"Last.Scan":16452,"Master.Scan.s.":16444,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_06.raw","File.ID":"F24","Quan.Info":"","Peptides.Matched":1955,"XCorr":1.88,"X..Protein.Groups":1,"Percolator.q.Value":0.00072,"Percolator.PEP":0.00558,"Percolator.SVMScore":0.682,"Precursor.Area":8129497.5,"Apex.RT..min.":15.7,"ptmRS..Binomial.Peptide.Score":217.03,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(2): 112.04; b1+-H(3) O(4) P(3): 241.08; b1+-H(3) O(4) P(4): 298.10; b1+-H(3) O(4) P(5): 411.19; b1+-H(3) O(4) P(6): 512.24; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35","ptmRS..Best.Site.Probabilities":"S2(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 100.0; T(6): 0.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (M4)","Search.ID":"M","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ELDEIAASLENL","Annotated.Sequence":"[K].ELDEIAAsLENL.[-]","Modifications":"S8(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6944,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":698.81436,"Contaminant":"False","Aligned.m.z..Da.":698.81433,"MH...Da.":1396.62145,"Theo..MH...Da.":1396.62433,"DeltaM..ppm.":-2.07,"Deltam.z..Da.":-0.00144,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1077683.625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":13.59169,"Ion.Inject.Time..ms.":22,"RT..min.":18.3492,"First.Scan":18999,"Last.Scan":18999,"Master.Scan.s.":18996,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_06.raw","File.ID":"F24","Quan.Info":"","Peptides.Matched":997,"XCorr":2.52,"X..Protein.Groups":1,"Percolator.q.Value":0.00072,"Percolator.PEP":0.00938,"Percolator.SVMScore":0.582,"Precursor.Area":746758.9375,"Apex.RT..min.":18.36,"ptmRS..Binomial.Peptide.Score":181.8,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"S8(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(8): 100.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (M4)","Search.ID":"M","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"GVFGYGFEEPSAIQQR","Annotated.Sequence":"[R].GVFGYGFEEPSAIQQR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.5503,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":595.62414,"Contaminant":"False","Aligned.m.z..Da.":595.62409,"MH...Da.":1784.85788,"Theo..MH...Da.":1784.86022,"DeltaM..ppm.":-1.31,"Deltam.z..Da.":-0.00078,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":210621.14062,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":6.04966,"Ion.Inject.Time..ms.":22,"RT..min.":15.0419,"First.Scan":15475,"Last.Scan":15475,"Master.Scan.s.":15474,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_06.raw","File.ID":"F24","Quan.Info":"","Peptides.Matched":1383,"XCorr":1.89,"X..Protein.Groups":1,"Percolator.q.Value":0.00072,"Percolator.PEP":0.00519,"Percolator.SVMScore":0.696,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (U4)","Search.ID":"U","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ILISTDLLAR","Annotated.Sequence":"[R].ILISTDLLAR.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5622,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":557.84543,"Contaminant":"False","Aligned.m.z..Da.":557.84539,"MH...Da.":1114.68359,"Theo..MH...Da.":1114.68304,"DeltaM..ppm.":0.5,"Deltam.z..Da.":0.00028,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1557034.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":16.15952,"Ion.Inject.Time..ms.":22,"RT..min.":15.5966,"First.Scan":15552,"Last.Scan":15552,"Master.Scan.s.":15541,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_04.raw","File.ID":"F16","Quan.Info":"","Peptides.Matched":124,"XCorr":2.49,"X..Protein.Groups":1,"Percolator.q.Value":0.00073,"Percolator.PEP":0.00023,"Percolator.SVMScore":1.32,"Precursor.Area":1341997.125,"Apex.RT..min.":15.6,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (U4)","Search.ID":"U","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LQQTAFDKK","Annotated.Sequence":"[R].LQQTAFDKK.[S]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.3077,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":360.20125,"Contaminant":"False","Aligned.m.z..Da.":360.20124,"MH...Da.":1078.58919,"Theo..MH...Da.":1078.58914,"DeltaM..ppm.":0.05,"Deltam.z..Da.":0.00002,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2904436.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":53.3142,"Ion.Inject.Time..ms.":22,"RT..min.":5.4846,"First.Scan":2240,"Last.Scan":2240,"Master.Scan.s.":2228,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_04.raw","File.ID":"F16","Quan.Info":"","Peptides.Matched":392,"XCorr":2.21,"X..Protein.Groups":1,"Percolator.q.Value":0.00073,"Percolator.PEP":0.00114,"Percolator.SVMScore":1.01,"Precursor.Area":1431581.125,"Apex.RT..min.":5.48,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (U4)","Search.ID":"U","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"GVFGYGFEEPSAIQQR","Annotated.Sequence":"[R].GVFGYGFEEPSAIQQR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.4914,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":595.62492,"Contaminant":"False","Aligned.m.z..Da.":595.62488,"MH...Da.":1784.86022,"Theo..MH...Da.":1784.86022,"DeltaM..ppm.":0,"Deltam.z..Da.":0,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":190685.39062,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":32.12701,"Ion.Inject.Time..ms.":22,"RT..min.":15.0357,"First.Scan":14857,"Last.Scan":14857,"Master.Scan.s.":14855,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_04.raw","File.ID":"F16","Quan.Info":"","Peptides.Matched":1272,"XCorr":1.75,"X..Protein.Groups":1,"Percolator.q.Value":0.00073,"Percolator.PEP":0.00058,"Percolator.SVMScore":1.142,"Precursor.Area":162790.10938,"Apex.RT..min.":15.03,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (U4)","Search.ID":"U","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KFQDDTLNR","Annotated.Sequence":"[K].KFQDDtLNR.[V]","Modifications":"T6(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6801,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":608.77253,"Contaminant":"False","Aligned.m.z..Da.":608.77253,"MH...Da.":1216.53779,"Theo..MH...Da.":1216.53579,"DeltaM..ppm.":1.64,"Deltam.z..Da.":0.001,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1670842.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":26.20125,"Ion.Inject.Time..ms.":22,"RT..min.":6.207,"First.Scan":3189,"Last.Scan":3189,"Master.Scan.s.":3177,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_04.raw","File.ID":"F16","Quan.Info":"","Peptides.Matched":638,"XCorr":2.97,"X..Protein.Groups":1,"Percolator.q.Value":0.00073,"Percolator.PEP":0.0002,"Percolator.SVMScore":1.349,"Precursor.Area":1403115.125,"Apex.RT..min.":6.21,"ptmRS..Binomial.Peptide.Score":339.88,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"T6(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"T(6): 100.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (U4)","Search.ID":"U","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DIFSNDELLSDAYDAK","Annotated.Sequence":"[K].DIFSNDELLSDAYDAK.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.6376,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":605.94826,"Contaminant":"False","Aligned.m.z..Da.":605.94828,"MH...Da.":1815.83022,"Theo..MH...Da.":1815.82831,"DeltaM..ppm.":1.05,"Deltam.z..Da.":0.00064,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":412615.78125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":10.61347,"Ion.Inject.Time..ms.":22,"RT..min.":17.0832,"First.Scan":17413,"Last.Scan":17413,"Master.Scan.s.":17409,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_04.raw","File.ID":"F16","Quan.Info":"","Peptides.Matched":1662,"XCorr":2.29,"X..Protein.Groups":1,"Percolator.q.Value":0.00073,"Percolator.PEP":0.00037,"Percolator.SVMScore":1.227,"Precursor.Area":413638.5,"Apex.RT..min.":17.09,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (U4)","Search.ID":"U","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KDELTLEGIK","Annotated.Sequence":"[K].KDELTLEGIK.[Q]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.578,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":382.55193,"Contaminant":"False","Aligned.m.z..Da.":382.55195,"MH...Da.":1145.64124,"Theo..MH...Da.":1145.64123,"DeltaM..ppm.":0.01,"Deltam.z..Da.":0,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1799865.375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":1.10531,"Ion.Inject.Time..ms.":22,"RT..min.":10.261,"First.Scan":8603,"Last.Scan":8603,"Master.Scan.s.":8598,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_04.raw","File.ID":"F16","Quan.Info":"","Peptides.Matched":307,"XCorr":2.82,"X..Protein.Groups":1,"Percolator.q.Value":0.00073,"Percolator.PEP":0.0006,"Percolator.SVMScore":1.135,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (W4)","Search.ID":"W","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LQQTAFDKK","Annotated.Sequence":"[R].LQQTAFDKK.[S]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.4198,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":360.20216,"Contaminant":"False","Aligned.m.z..Da.":360.20212,"MH...Da.":1078.59194,"Theo..MH...Da.":1078.58914,"DeltaM..ppm.":2.6,"Deltam.z..Da.":0.00094,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2071804.875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":38.73754,"Ion.Inject.Time..ms.":22,"RT..min.":5.5009,"First.Scan":2300,"Last.Scan":2300,"Master.Scan.s.":2289,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_02.raw","File.ID":"F14","Quan.Info":"","Peptides.Matched":384,"XCorr":1.62,"X..Protein.Groups":1,"Percolator.q.Value":0.00073,"Percolator.PEP":0.00695,"Percolator.SVMScore":0.679,"Precursor.Area":1252051.625,"Apex.RT..min.":5.48,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (U4)","Search.ID":"U","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ILISTDLLAR","Annotated.Sequence":"[R].ILISTDLLAR.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5779,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":557.84574,"Contaminant":"False","Aligned.m.z..Da.":557.84569,"MH...Da.":1114.6842,"Theo..MH...Da.":1114.68304,"DeltaM..ppm.":1.05,"Deltam.z..Da.":0.00058,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":394678.4375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":49.297,"Ion.Inject.Time..ms.":22,"RT..min.":15.6319,"First.Scan":15594,"Last.Scan":15594,"Master.Scan.s.":15593,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_04.raw","File.ID":"F16","Quan.Info":"","Peptides.Matched":124,"XCorr":1.54,"X..Protein.Groups":1,"Percolator.q.Value":0.00074,"Percolator.PEP":0.002,"Percolator.SVMScore":0.9,"Precursor.Area":1341997.125,"Apex.RT..min.":15.6,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (U4)","Search.ID":"U","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MGSINNNFNTNNNSNTDLDR","Annotated.Sequence":"[-].mGSINNNFNTNNNsNTDLDR.[D]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S14(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P39517","Master.Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","Protein.Accessions":"P39517","Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0553,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":749.3099,"Contaminant":"False","Aligned.m.z..Da.":749.31008,"MH...Da.":2245.91517,"Theo..MH...Da.":2245.91057,"DeltaM..ppm.":2.05,"Deltam.z..Da.":0.00153,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":717195.6875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":36.25773,"Ion.Inject.Time..ms.":22,"RT..min.":11.9933,"First.Scan":10945,"Last.Scan":10945,"Master.Scan.s.":10938,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_04.raw","File.ID":"F16","Quan.Info":"","Peptides.Matched":1482,"XCorr":2.17,"X..Protein.Groups":1,"Percolator.q.Value":0.00074,"Percolator.PEP":0.00342,"Percolator.SVMScore":0.796,"Precursor.Area":383933,"Apex.RT..min.":11.99,"ptmRS..Binomial.Peptide.Score":180.47,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(12): 1359.59; y1+-H(3) O(4) P(10): 1144.50; y1+-H(3) O(4) P(8): 916.41; y1+-H(3) O(4) P(7): 802.37; y1+(6): 733.35; y1+(5): 619.30","ptmRS..Best.Site.Probabilities":"S14(Phospho): 99.99","ptmRS..Phospho.Site.Probabilities":"S(3): 0.0; T(10): 0.0; S(14): 100.0; T(16): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AC4)","Search.ID":"AC","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"SFLTYIK","Annotated.Sequence":"[K].SFLTYIK.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6331,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":436.24858,"Contaminant":"False","Aligned.m.z..Da.":436.24857,"MH...Da.":871.48989,"Theo..MH...Da.":871.49238,"DeltaM..ppm.":-2.86,"Deltam.z..Da.":-0.00125,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1094555.875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":13.6799,"First.Scan":13075,"Last.Scan":13075,"Master.Scan.s.":13072,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_02.raw","File.ID":"F8","Quan.Info":"","Peptides.Matched":287,"XCorr":1.39,"X..Protein.Groups":1,"Percolator.q.Value":0.00074,"Percolator.PEP":0.01438,"Percolator.SVMScore":0.421,"Precursor.Area":2020683.125,"Apex.RT..min.":13.7,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (X4)","Search.ID":"X","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ILISTDLLAR","Annotated.Sequence":"[R].ILISTDLLAR.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5273,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":557.84519,"Contaminant":"False","Aligned.m.z..Da.":557.84516,"MH...Da.":1114.68312,"Theo..MH...Da.":1114.68304,"DeltaM..ppm.":0.07,"Deltam.z..Da.":0.00004,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":53708.05859,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":52.142,"Ion.Inject.Time..ms.":22,"RT..min.":15.6505,"First.Scan":15126,"Last.Scan":15126,"Master.Scan.s.":15124,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_01.raw","File.ID":"F13","Quan.Info":"","Peptides.Matched":124,"XCorr":1.1,"X..Protein.Groups":1,"Percolator.q.Value":0.00076,"Percolator.PEP":0.01286,"Percolator.SVMScore":0.574,"Precursor.Area":671347.25,"Apex.RT..min.":15.6,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (P4)","Search.ID":"P","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KVIGSFK","Annotated.Sequence":"[K].KVIGSFK.[D]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.3706,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":389.74444,"Contaminant":"False","Aligned.m.z..Da.":389.74443,"MH...Da.":778.4816,"Theo..MH...Da.":778.48215,"DeltaM..ppm.":-0.71,"Deltam.z..Da.":-0.00028,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2201533.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":65.35581,"Ion.Inject.Time..ms.":22,"RT..min.":6.2874,"First.Scan":3249,"Last.Scan":3249,"Master.Scan.s.":3241,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_03.raw","File.ID":"F21","Quan.Info":"","Peptides.Matched":22,"XCorr":1.97,"X..Protein.Groups":1,"Percolator.q.Value":0.00076,"Percolator.PEP":0.01627,"Percolator.SVMScore":0.472,"Precursor.Area":2404144.25,"Apex.RT..min.":6.3,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ELAYQIR","Annotated.Sequence":"[R].ELAYQIR.[N]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.3366,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":446.74789,"Contaminant":"False","Aligned.m.z..Da.":446.74789,"MH...Da.":892.4885,"Theo..MH...Da.":892.48869,"DeltaM..ppm.":-0.22,"Deltam.z..Da.":-0.0001,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":9323329,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":42.05545,"Ion.Inject.Time..ms.":8.751,"RT..min.":9.5528,"First.Scan":7922,"Last.Scan":7922,"Master.Scan.s.":7919,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":402,"XCorr":2.05,"X..Protein.Groups":1,"Percolator.q.Value":0.00077,"Percolator.PEP":0.00882,"Percolator.SVMScore":0.548,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"SFLTYIK","Annotated.Sequence":"[K].SFLTYIK.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6042,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":436.24972,"Contaminant":"False","Aligned.m.z..Da.":436.24972,"MH...Da.":871.49216,"Theo..MH...Da.":871.49238,"DeltaM..ppm.":-0.25,"Deltam.z..Da.":-0.00011,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3615688.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":1.14542,"Ion.Inject.Time..ms.":22,"RT..min.":13.7028,"First.Scan":13317,"Last.Scan":13317,"Master.Scan.s.":13314,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":273,"XCorr":1.92,"X..Protein.Groups":1,"Percolator.q.Value":0.00077,"Percolator.PEP":0.00897,"Percolator.SVMScore":0.545,"Precursor.Area":48034940,"Apex.RT..min.":13.74,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (I4)","Search.ID":"I","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"IQDPQMTGYVSTR","Annotated.Sequence":"[R].IQDPQMtGYVsTR.[Y]","Modifications":"T7(Phospho); S11(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P32485","Master.Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","Protein.Accessions":"P32485","Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.0355,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":828.33004,"Contaminant":"False","Aligned.m.z..Da.":828.32999,"MH...Da.":1655.65282,"Theo..MH...Da.":1655.65362,"DeltaM..ppm.":-0.48,"Deltam.z..Da.":-0.0004,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1871102.375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":80.06665,"Ion.Inject.Time..ms.":19.368,"RT..min.":9.9128,"First.Scan":8224,"Last.Scan":8224,"Master.Scan.s.":8212,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_04.raw","File.ID":"F28","Quan.Info":"","Peptides.Matched":734,"XCorr":1.69,"X..Protein.Groups":1,"Percolator.q.Value":0.00078,"Percolator.PEP":0.0061,"Percolator.SVMScore":0.648,"Precursor.Area":1586210.625,"Apex.RT..min.":9.91,"ptmRS..Binomial.Peptide.Score":230.89,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(11): 1316.53; y1+-H(3) O(4) P(10): 1201.51; y1+-H(3) O(4) P(8): 976.40; y1+-H(3) O(4) P(7): 845.36; b2+-H(3) O(4) P(11): 601.78; y2+-H(3) O(4) P(10): 601.26; y1+- -2H(3) O(4) P -2H(3) O(4) P(10): 1103.53; b2+(11): 650.77","ptmRS..Best.Site.Probabilities":"T7(Phospho): 99.24; T12(Phospho): 99.23","ptmRS..Phospho.Site.Probabilities":"T(7): 99.2; Y(9): 0.8; S(11): 0.8; T(12): 99.2"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (O4)","Search.ID":"O","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].msHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss); S2(Phospho); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.3978,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":980.0566,"Contaminant":"False","Aligned.m.z..Da.":980.0566,"MH...Da.":2938.15527,"Theo..MH...Da.":2938.15509,"DeltaM..ppm.":0.06,"Deltam.z..Da.":0.00006,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":359447.3125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":27.45068,"Ion.Inject.Time..ms.":22,"RT..min.":15.1014,"First.Scan":14978,"Last.Scan":14978,"Master.Scan.s.":14973,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_04.raw","File.ID":"F22","Quan.Info":"","Peptides.Matched":1348,"XCorr":1.86,"X..Protein.Groups":1,"Percolator.q.Value":0.00078,"Percolator.PEP":0.01225,"Percolator.SVMScore":0.536,"ptmRS..Binomial.Peptide.Score":163.47,"ptmRS..Isoform.Confidence.Probability":0.5,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"S2(Phospho): 99.98; Y12(Phospho): 50.01; S13(Phospho): 50.01","ptmRS..Phospho.Site.Probabilities":"S(2): 100.0; Y(12): 50.0; S(13): 50.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (K4)","Search.ID":"K","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ELDEIAASLENL","Annotated.Sequence":"[K].ELDEIAASLENL.[-]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5374,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":658.83281,"Contaminant":"False","Aligned.m.z..Da.":658.83259,"MH...Da.":1316.65835,"Theo..MH...Da.":1316.658,"DeltaM..ppm.":0.26,"Deltam.z..Da.":0.00017,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":746772,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":23.911,"Ion.Inject.Time..ms.":22,"RT..min.":18.449,"First.Scan":19235,"Last.Scan":19235,"Master.Scan.s.":19228,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_02.raw","File.ID":"F26","Quan.Info":"","Peptides.Matched":693,"XCorr":2.27,"X..Protein.Groups":1,"Percolator.q.Value":0.00078,"Percolator.PEP":0.00385,"Percolator.SVMScore":0.816,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (K4)","Search.ID":"K","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].msEGITDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S2(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":3,"DeltaScore":0.22,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1096.46763,"Contaminant":"False","Aligned.m.z..Da.":1096.46749,"MH...Da.":2191.92799,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":0.07,"Deltam.z..Da.":0.00007,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":732370,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":72.86369,"Ion.Inject.Time..ms.":22,"RT..min.":15.9045,"First.Scan":15977,"Last.Scan":15977,"Master.Scan.s.":15968,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_02.raw","File.ID":"F26","Quan.Info":"","Peptides.Matched":1955,"XCorr":2,"X..Protein.Groups":1,"Percolator.q.Value":0.00078,"Percolator.PEP":0.00405,"Percolator.SVMScore":0.806,"Precursor.Area":21676970,"Apex.RT..min.":15.78,"ptmRS..Binomial.Peptide.Score":145.85,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(2): 112.04; b1+-H(3) O(4) P(3): 241.08; b1+-H(3) O(4) P(4): 298.10; b1+-H(3) O(4) P(5): 411.19; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35","ptmRS..Best.Site.Probabilities":"S2(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 100.0; T(6): 0.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (H4)","Search.ID":"H","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ELDEIAASLENL","Annotated.Sequence":"[K].ELDEIAAsLENL.[-]","Modifications":"S8(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6095,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":698.81511,"Contaminant":"False","Aligned.m.z..Da.":698.81494,"MH...Da.":1396.62296,"Theo..MH...Da.":1396.62433,"DeltaM..ppm.":-0.99,"Deltam.z..Da.":-0.00069,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":573822.125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":18.3073,"First.Scan":19113,"Last.Scan":19113,"Master.Scan.s.":19101,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_05.raw","File.ID":"F29","Quan.Info":"","Peptides.Matched":922,"XCorr":2.1,"X..Protein.Groups":1,"Percolator.q.Value":0.0008,"Percolator.PEP":0.01024,"Percolator.SVMScore":0.571,"ptmRS..Binomial.Peptide.Score":202.7,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"S8(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(8): 100.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (H4)","Search.ID":"H","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"TLIGYGR","Annotated.Sequence":"[R].TLIGYGR.[F]","Modifications":"","X..Proteins":2,"Master.Protein.Accessions":"Q03558","Master.Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","Protein.Accessions":"Q03558; P41816","Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3; NADPH dehydrogenase 3 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE3 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.3394,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":390.22354,"Contaminant":"False","Aligned.m.z..Da.":390.22357,"MH...Da.":779.43981,"Theo..MH...Da.":779.44101,"DeltaM..ppm.":-1.54,"Deltam.z..Da.":-0.0006,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2760811.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":28.88889,"Ion.Inject.Time..ms.":22,"RT..min.":9.1455,"First.Scan":7818,"Last.Scan":7818,"Master.Scan.s.":7811,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_05.raw","File.ID":"F29","Quan.Info":"","Peptides.Matched":75,"XCorr":1.65,"X..Protein.Groups":1,"Percolator.q.Value":0.0008,"Percolator.PEP":0.01109,"Percolator.SVMScore":0.557,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LQQTAFDKK","Annotated.Sequence":"[R].LQQTAFDKK.[S]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.3527,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":360.20156,"Contaminant":"False","Aligned.m.z..Da.":360.20158,"MH...Da.":1078.59012,"Theo..MH...Da.":1078.58914,"DeltaM..ppm.":0.92,"Deltam.z..Da.":0.00033,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3008509.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":32.24385,"Ion.Inject.Time..ms.":22,"RT..min.":5.6057,"First.Scan":2988,"Last.Scan":2988,"Master.Scan.s.":2984,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":392,"XCorr":2.07,"X..Protein.Groups":1,"Percolator.q.Value":0.0008,"Percolator.PEP":0.00592,"Percolator.SVMScore":0.669,"Precursor.Area":47125128,"Apex.RT..min.":5.5,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSSPLHRLSPTGK","Annotated.Sequence":"[R].MSsPLHRLsPTGK.[S]","Modifications":"S3(Phospho); S9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":3,"DeltaScore":0.3316,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":785.84615,"Contaminant":"False","Aligned.m.z..Da.":785.84621,"MH...Da.":1570.68502,"Theo..MH...Da.":1570.68486,"DeltaM..ppm.":0.1,"Deltam.z..Da.":0.00008,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1667342,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":90.99195,"Ion.Inject.Time..ms.":22,"RT..min.":8.6546,"First.Scan":6734,"Last.Scan":6734,"Master.Scan.s.":6728,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":1054,"XCorr":1.87,"X..Protein.Groups":1,"Percolator.q.Value":0.0008,"Percolator.PEP":0.0061,"Percolator.SVMScore":0.662,"ptmRS..Binomial.Peptide.Score":195.91,"ptmRS..Isoform.Confidence.Probability":0.99,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(9): 1071.48; y2+- -2H(3) O(4) P -2H(3) O(4) P(11): 578.83","ptmRS..Best.Site.Probabilities":"S3(Phospho): 99.03; S9(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 1.0; S(3): 99.0; S(9): 100.0; T(11): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"QLSEDSHHLQSNNDNFSK","Annotated.Sequence":"[K].QLSEDSHHLQSNNDNFsK.[K]","Modifications":"S17(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.1497,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":727.30706,"Contaminant":"False","Aligned.m.z..Da.":727.30706,"MH...Da.":2179.90662,"Theo..MH...Da.":2179.90403,"DeltaM..ppm.":1.19,"Deltam.z..Da.":0.00086,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":4087509,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":20.06565,"Ion.Inject.Time..ms.":22,"RT..min.":6.8383,"First.Scan":4243,"Last.Scan":4243,"Master.Scan.s.":4232,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":1864,"XCorr":3.14,"X..Protein.Groups":1,"Percolator.q.Value":0.0008,"Percolator.PEP":0.00599,"Percolator.SVMScore":0.667,"ptmRS..Binomial.Peptide.Score":240.07,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(11): 1285.59; y1+-H(3) O(4) P(10): 1148.53; y1+-H(3) O(4) P(9): 1035.45; y1+-H(3) O(4) P(8): 907.39; y1+-H(3) O(4) P(3): 363.20; y1+-H(3) O(4) P(2): 216.13; y2+-H(3) O(4) P(16): 920.90; y2+-H(3) O(4) P(14): 812.86; y2+-H(3) O(4) P(13): 755.35; y2+-H(3) O(4) P(11): 643.30","ptmRS..Best.Site.Probabilities":"S17(Phospho): 99.99","ptmRS..Phospho.Site.Probabilities":"S(3): 0.0; S(6): 0.0; S(11): 0.0; S(17): 100.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"GITQYYAFVEER","Annotated.Sequence":"[K].GITQYYAFVEER.[Q]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P39517","Master.Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","Protein.Accessions":"P39517","Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.3071,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":738.35874,"Contaminant":"False","Aligned.m.z..Da.":738.35888,"MH...Da.":1475.71021,"Theo..MH...Da.":1475.71652,"DeltaM..ppm.":-4.28,"Deltam.z..Da.":-0.00316,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":150751.625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":86.6416,"Ion.Inject.Time..ms.":22,"RT..min.":14.926,"First.Scan":14851,"Last.Scan":14851,"Master.Scan.s.":14840,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":906,"XCorr":1.4,"X..Protein.Groups":1,"Percolator.q.Value":0.0008,"Percolator.PEP":0.0062,"Percolator.SVMScore":0.659,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Q4)","Search.ID":"Q","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"SFLTYIK","Annotated.Sequence":"[K].SFLTYIK.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.642,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":436.24945,"Contaminant":"False","Aligned.m.z..Da.":436.2496,"MH...Da.":871.49164,"Theo..MH...Da.":871.49238,"DeltaM..ppm.":-0.85,"Deltam.z..Da.":-0.00037,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1765715.125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":1.49912,"Ion.Inject.Time..ms.":22,"RT..min.":13.6704,"First.Scan":13125,"Last.Scan":13125,"Master.Scan.s.":13122,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_02.raw","File.ID":"F20","Quan.Info":"","Peptides.Matched":273,"XCorr":1.62,"X..Protein.Groups":1,"Percolator.q.Value":0.00081,"Percolator.PEP":0.01052,"Percolator.SVMScore":0.636,"Precursor.Area":6000368,"Apex.RT..min.":13.7,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Q4)","Search.ID":"Q","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].msEGITDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S2(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":3,"DeltaScore":0.1183,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1096.47001,"Contaminant":"False","Aligned.m.z..Da.":1096.46996,"MH...Da.":2191.93275,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":2.24,"Deltam.z..Da.":0.00245,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":250391.40625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":87.94344,"Ion.Inject.Time..ms.":22,"RT..min.":16.0565,"First.Scan":16102,"Last.Scan":16102,"Master.Scan.s.":16098,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_02.raw","File.ID":"F20","Quan.Info":"","Peptides.Matched":2110,"XCorr":1.86,"X..Protein.Groups":1,"Percolator.q.Value":0.00081,"Percolator.PEP":0.01034,"Percolator.SVMScore":0.639,"ptmRS..Binomial.Peptide.Score":178.27,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(2): 112.04; b1+-H(3) O(4) P(3): 241.08; b1+-H(3) O(4) P(4): 298.10; b1+-H(3) O(4) P(5): 411.19; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+-H(3) O(4) P(9): 869.39","ptmRS..Best.Site.Probabilities":"S2(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 100.0; T(6): 0.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (J4)","Search.ID":"J","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"IQDPQMTGYVSTR","Annotated.Sequence":"[R].IQDPQMtGYVsTR.[Y]","Modifications":"T7(Phospho); S11(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P32485","Master.Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","Protein.Accessions":"P32485","Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.0394,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":828.32942,"Contaminant":"False","Aligned.m.z..Da.":828.32943,"MH...Da.":1655.65157,"Theo..MH...Da.":1655.65362,"DeltaM..ppm.":-1.24,"Deltam.z..Da.":-0.00102,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3035791.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":59.29926,"Ion.Inject.Time..ms.":22,"RT..min.":9.8984,"First.Scan":8163,"Last.Scan":8163,"Master.Scan.s.":8157,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_03.raw","File.ID":"F27","Quan.Info":"","Peptides.Matched":750,"XCorr":2.03,"X..Protein.Groups":1,"Percolator.q.Value":0.00081,"Percolator.PEP":0.00443,"Percolator.SVMScore":0.719,"Precursor.Area":1929041,"Apex.RT..min.":9.9,"ptmRS..Binomial.Peptide.Score":268.63,"ptmRS..Isoform.Confidence.Probability":0.003,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(11): 1316.53; y1+-H(3) O(4) P(10): 1201.51; y1+-H(3) O(4) P(8): 976.40; y1+-H(3) O(4) P(7): 845.36; y2+-H(3) O(4) P(10): 601.26; y1+(4): 462.27; y1+(3): 363.20","ptmRS..Best.Site.Probabilities":"T7(Phospho): 99.7; Y9(Phospho): 49.7; T12(Phospho): 50","ptmRS..Phospho.Site.Probabilities":"T(7): 99.7; Y(9): 49.7; S(11): 0.6; T(12): 50.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AI4)","Search.ID":"AI","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSSPLHRLSPTGK","Annotated.Sequence":"[R].mSsPLHRLsPTGK.[S]","Modifications":"M1(Oxidation); S3(Phospho); S9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.4309,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":529.56472,"Contaminant":"False","Aligned.m.z..Da.":529.56478,"MH...Da.":1586.67961,"Theo..MH...Da.":1586.67977,"DeltaM..ppm.":-0.1,"Deltam.z..Da.":-0.00005,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1062563.875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":48.91928,"Ion.Inject.Time..ms.":22,"RT..min.":7.7416,"First.Scan":5458,"Last.Scan":5458,"Master.Scan.s.":5446,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y25_02.raw","File.ID":"F2","Quan.Info":"","Peptides.Matched":986,"XCorr":1.88,"X..Protein.Groups":1,"Percolator.q.Value":0.00082,"Percolator.PEP":0.01328,"Percolator.SVMScore":0.548,"ptmRS..Binomial.Peptide.Score":159.19,"ptmRS..Isoform.Confidence.Probability":0.989,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(8): 877.50; y2+-H(3) O(4) P(11): 627.82; y2+-H(3) O(4) P(10): 544.32; y2+-H(3) O(4) P(8): 439.25; y1+(4): 402.23","ptmRS..Best.Site.Probabilities":"S3(Phospho): 99.99; S9(Phospho): 98.9","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(3): 100.0; S(9): 98.9; T(11): 1.1"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (U4)","Search.ID":"U","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"SFLTYIK","Annotated.Sequence":"[K].SFLTYIK.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6541,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":436.24967,"Contaminant":"False","Aligned.m.z..Da.":436.24969,"MH...Da.":871.49207,"Theo..MH...Da.":871.49238,"DeltaM..ppm.":-0.36,"Deltam.z..Da.":-0.00016,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":803074.8125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":3.59917,"Ion.Inject.Time..ms.":22,"RT..min.":13.6696,"First.Scan":13177,"Last.Scan":13177,"Master.Scan.s.":13174,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_04.raw","File.ID":"F16","Quan.Info":"","Peptides.Matched":273,"XCorr":1.59,"X..Protein.Groups":1,"Percolator.q.Value":0.00083,"Percolator.PEP":0.007,"Percolator.SVMScore":0.658,"Precursor.Area":4737163,"Apex.RT..min.":13.7,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AG4)","Search.ID":"AG","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ELALQIQK","Annotated.Sequence":"[R].ELALQIQK.[V]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.227,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":471.78486,"Contaminant":"False","Aligned.m.z..Da.":471.78485,"MH...Da.":942.56245,"Theo..MH...Da.":942.56186,"DeltaM..ppm.":0.62,"Deltam.z..Da.":0.00029,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":531541.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":12.55141,"Ion.Inject.Time..ms.":22,"RT..min.":10.5853,"First.Scan":9102,"Last.Scan":9102,"Master.Scan.s.":9097,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y25_04.raw","File.ID":"F4","Quan.Info":"","Peptides.Matched":292,"XCorr":1.85,"X..Protein.Groups":1,"Percolator.q.Value":0.00083,"Percolator.PEP":0.00909,"Percolator.SVMScore":0.573,"Precursor.Area":404737.375,"Apex.RT..min.":10.58,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (V4)","Search.ID":"V","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LQQTAFDKK","Annotated.Sequence":"[R].LQQTAFDKK.[S]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.259,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":360.20107,"Contaminant":"False","Aligned.m.z..Da.":360.20106,"MH...Da.":1078.58866,"Theo..MH...Da.":1078.58914,"DeltaM..ppm.":-0.44,"Deltam.z..Da.":-0.00016,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2078731.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":55.45574,"Ion.Inject.Time..ms.":22,"RT..min.":5.495,"First.Scan":2249,"Last.Scan":2249,"Master.Scan.s.":2241,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_03.raw","File.ID":"F15","Quan.Info":"","Peptides.Matched":388,"XCorr":1.39,"X..Protein.Groups":1,"Percolator.q.Value":0.00084,"Percolator.PEP":0.00439,"Percolator.SVMScore":0.645,"Precursor.Area":1308901,"Apex.RT..min.":5.49,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (A4)","Search.ID":"A","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FQDDTLNR","Annotated.Sequence":"[K].FQDDtLNR.[V]","Modifications":"T5(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6074,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":544.72362,"Contaminant":"False","Aligned.m.z..Da.":544.72367,"MH...Da.":1088.43997,"Theo..MH...Da.":1088.44083,"DeltaM..ppm.":-0.79,"Deltam.z..Da.":-0.00043,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1703816.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":59.88871,"Ion.Inject.Time..ms.":22,"RT..min.":7.3364,"First.Scan":5066,"Last.Scan":5066,"Master.Scan.s.":5064,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_03.raw","File.ID":"F36","Quan.Info":"","Peptides.Matched":423,"XCorr":1.63,"X..Protein.Groups":1,"Percolator.q.Value":0.00085,"Percolator.PEP":0.01615,"Percolator.SVMScore":0.507,"Precursor.Area":1248843.5,"Apex.RT..min.":7.33,"ptmRS..Binomial.Peptide.Score":154.28,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"T5(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"T(5): 100.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ALEYLEK","Annotated.Sequence":"[K].ALEYLEK.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q08421","Master.Protein.Descriptions":"Enhancer of translation termination 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ETT1 PE=1 SV=1","Protein.Accessions":"Q08421","Protein.Descriptions":"Enhancer of translation termination 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ETT1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":3,"DeltaScore":0.0611,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":2,"m.z..Da.":433.23718,"Contaminant":"False","Aligned.m.z..Da.":433.23718,"MH...Da.":865.46708,"Theo..MH...Da.":865.46656,"DeltaM..ppm.":0.6,"Deltam.z..Da.":0.00026,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":451635.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":94.95772,"Ion.Inject.Time..ms.":15.805,"RT..min.":9.4429,"First.Scan":7778,"Last.Scan":7778,"Master.Scan.s.":7776,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":234,"XCorr":1.31,"X..Protein.Groups":1,"Percolator.q.Value":0.00086,"Percolator.PEP":0.00964,"Percolator.SVMScore":0.532,"Precursor.Area":929327.1875,"Apex.RT..min.":9.45,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (P4)","Search.ID":"P","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"SFLTYIK","Annotated.Sequence":"[K].SFLTYIK.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6952,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":436.25003,"Contaminant":"False","Aligned.m.z..Da.":436.25009,"MH...Da.":871.49278,"Theo..MH...Da.":871.49238,"DeltaM..ppm.":0.46,"Deltam.z..Da.":0.0002,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1608576.625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":13.6724,"First.Scan":13150,"Last.Scan":13150,"Master.Scan.s.":13147,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_03.raw","File.ID":"F21","Quan.Info":"","Peptides.Matched":60,"XCorr":1.87,"X..Protein.Groups":1,"Percolator.q.Value":0.00086,"Percolator.PEP":0.02109,"Percolator.SVMScore":0.424,"Precursor.Area":6054581,"Apex.RT..min.":13.7,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (P4)","Search.ID":"P","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"VFDNIQR","Annotated.Sequence":"[R].VFDNIQR.[R]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.329,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":446.23786,"Contaminant":"False","Aligned.m.z..Da.":446.23794,"MH...Da.":891.46844,"Theo..MH...Da.":891.46829,"DeltaM..ppm.":0.17,"Deltam.z..Da.":0.00007,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":5188736,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":64.42112,"Ion.Inject.Time..ms.":11.898,"RT..min.":8.2817,"First.Scan":6274,"Last.Scan":6274,"Master.Scan.s.":6270,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_03.raw","File.ID":"F21","Quan.Info":"","Peptides.Matched":426,"XCorr":1.55,"X..Protein.Groups":1,"Percolator.q.Value":0.00086,"Percolator.PEP":0.02092,"Percolator.SVMScore":0.425,"Precursor.Area":4227335,"Apex.RT..min.":8.27,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AJ4)","Search.ID":"AJ","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DAQIVVGTPGR","Annotated.Sequence":"[R].DAQIVVGTPGR.[V]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":3,"DeltaScore":0.416,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":556.80635,"Contaminant":"False","Aligned.m.z..Da.":556.80646,"MH...Da.":1112.60543,"Theo..MH...Da.":1112.60585,"DeltaM..ppm.":-0.37,"Deltam.z..Da.":-0.00021,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":139040.4375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":87.70308,"Ion.Inject.Time..ms.":22,"RT..min.":9.1708,"First.Scan":7388,"Last.Scan":7388,"Master.Scan.s.":7378,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y25_01.raw","File.ID":"F1","Quan.Info":"","Peptides.Matched":249,"XCorr":1.25,"X..Protein.Groups":1,"Percolator.q.Value":0.00088,"Percolator.PEP":0.00876,"Percolator.SVMScore":0.406,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (L4)","Search.ID":"L","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"VFDNIQR","Annotated.Sequence":"[R].VFDNIQR.[R]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.4521,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":446.23849,"Contaminant":"False","Aligned.m.z..Da.":446.23856,"MH...Da.":891.4697,"Theo..MH...Da.":891.46829,"DeltaM..ppm.":1.58,"Deltam.z..Da.":0.0007,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":4873182,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":38.22601,"Ion.Inject.Time..ms.":16.133,"RT..min.":8.2495,"First.Scan":6230,"Last.Scan":6230,"Master.Scan.s.":6223,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_01.raw","File.ID":"F25","Quan.Info":"","Peptides.Matched":362,"XCorr":2.19,"X..Protein.Groups":1,"Percolator.q.Value":0.00089,"Percolator.PEP":0.01224,"Percolator.SVMScore":0.621,"Precursor.Area":5335867.5,"Apex.RT..min.":8.26,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"VKSPLDDK","Annotated.Sequence":"[K].VKsPLDDK.[L]","Modifications":"S3(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6542,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":491.23612,"Contaminant":"False","Aligned.m.z..Da.":491.23613,"MH...Da.":981.46496,"Theo..MH...Da.":981.46525,"DeltaM..ppm.":-0.3,"Deltam.z..Da.":-0.00015,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":7807989.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":7.00079,"Ion.Inject.Time..ms.":18.342,"RT..min.":4.9264,"First.Scan":2127,"Last.Scan":2127,"Master.Scan.s.":2126,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":508,"XCorr":2.4,"X..Protein.Groups":1,"Percolator.q.Value":0.00091,"Percolator.PEP":0.00652,"Percolator.SVMScore":0.646,"Precursor.Area":5107614,"Apex.RT..min.":4.93,"ptmRS..Binomial.Peptide.Score":317.27,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"S3(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(3): 100.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ELDEIAASLENL","Annotated.Sequence":"[K].ELDEIAAsLENL.[-]","Modifications":"S8(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6477,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":698.81591,"Contaminant":"False","Aligned.m.z..Da.":698.81596,"MH...Da.":1396.62454,"Theo..MH...Da.":1396.62433,"DeltaM..ppm.":0.15,"Deltam.z..Da.":0.00011,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1799627.125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":18.2299,"First.Scan":19082,"Last.Scan":19082,"Master.Scan.s.":19078,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":870,"XCorr":2.64,"X..Protein.Groups":1,"Percolator.q.Value":0.00091,"Percolator.PEP":0.00691,"Percolator.SVMScore":0.633,"Precursor.Area":2599102.5,"Apex.RT..min.":18.24,"ptmRS..Binomial.Peptide.Score":206.84,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"S8(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(8): 100.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (W4)","Search.ID":"W","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"VFDNIQR","Annotated.Sequence":"[R].VFDNIQR.[R]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.3949,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":446.23827,"Contaminant":"False","Aligned.m.z..Da.":446.23835,"MH...Da.":891.46927,"Theo..MH...Da.":891.46829,"DeltaM..ppm.":1.1,"Deltam.z..Da.":0.00049,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":4596847.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":45.81873,"Ion.Inject.Time..ms.":19.227,"RT..min.":8.2572,"First.Scan":6231,"Last.Scan":6231,"Master.Scan.s.":6228,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_02.raw","File.ID":"F14","Quan.Info":"","Peptides.Matched":368,"XCorr":1.95,"X..Protein.Groups":1,"Percolator.q.Value":0.00093,"Percolator.PEP":0.01089,"Percolator.SVMScore":0.586,"Precursor.Area":2570825.75,"Apex.RT..min.":8.26,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (W4)","Search.ID":"W","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"VFDNIQR","Annotated.Sequence":"[R].VFDNIQR.[R]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.3943,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":446.23827,"Contaminant":"False","Aligned.m.z..Da.":446.23835,"MH...Da.":891.46927,"Theo..MH...Da.":891.46829,"DeltaM..ppm.":1.09,"Deltam.z..Da.":0.00049,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":4596847.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":45.81873,"Ion.Inject.Time..ms.":5.731,"RT..min.":8.2591,"First.Scan":6234,"Last.Scan":6234,"Master.Scan.s.":6228,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_02.raw","File.ID":"F14","Quan.Info":"","Peptides.Matched":368,"XCorr":1.75,"X..Protein.Groups":1,"Percolator.q.Value":0.00093,"Percolator.PEP":0.01184,"Percolator.SVMScore":0.569,"Precursor.Area":2570825.75,"Apex.RT..min.":8.26,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (V4)","Search.ID":"V","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"VFDNIQR","Annotated.Sequence":"[R].VFDNIQR.[R]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.3971,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":446.23839,"Contaminant":"False","Aligned.m.z..Da.":446.23847,"MH...Da.":891.4695,"Theo..MH...Da.":891.46829,"DeltaM..ppm.":1.36,"Deltam.z..Da.":0.0006,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2996297.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":46.31865,"Ion.Inject.Time..ms.":22,"RT..min.":8.2425,"First.Scan":6235,"Last.Scan":6235,"Master.Scan.s.":6232,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_03.raw","File.ID":"F15","Quan.Info":"","Peptides.Matched":363,"XCorr":2.09,"X..Protein.Groups":1,"Percolator.q.Value":0.00095,"Percolator.PEP":0.00656,"Percolator.SVMScore":0.566,"Precursor.Area":2799589.5,"Apex.RT..min.":8.25,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (V4)","Search.ID":"V","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ELDEIAASLENL","Annotated.Sequence":"[K].ELDEIAASLENL.[-]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.4973,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":658.83366,"Contaminant":"False","Aligned.m.z..Da.":658.83347,"MH...Da.":1316.66005,"Theo..MH...Da.":1316.658,"DeltaM..ppm.":1.55,"Deltam.z..Da.":0.00102,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":677302.6875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":8.77892,"Ion.Inject.Time..ms.":22,"RT..min.":18.4593,"First.Scan":19081,"Last.Scan":19081,"Master.Scan.s.":19071,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_03.raw","File.ID":"F15","Quan.Info":"","Peptides.Matched":660,"XCorr":1.85,"X..Protein.Groups":1,"Percolator.q.Value":0.00095,"Percolator.PEP":0.007,"Percolator.SVMScore":0.554,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (I4)","Search.ID":"I","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"QLSEDSHHLQSNNDNFSK","Annotated.Sequence":"[K].QLSEDSHHLQSNNDNFsK.[K]","Modifications":"S17(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.1722,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":727.30764,"Contaminant":"False","Aligned.m.z..Da.":727.30762,"MH...Da.":2179.90838,"Theo..MH...Da.":2179.90403,"DeltaM..ppm.":1.99,"Deltam.z..Da.":0.00145,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1065665.625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":6.9988,"First.Scan":4503,"Last.Scan":4503,"Master.Scan.s.":4494,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_04.raw","File.ID":"F28","Quan.Info":"","Peptides.Matched":1921,"XCorr":3.31,"X..Protein.Groups":1,"Percolator.q.Value":0.00098,"Percolator.PEP":0.00889,"Percolator.SVMScore":0.571,"Precursor.Area":1193437.5,"Apex.RT..min.":6.99,"ptmRS..Binomial.Peptide.Score":251.84,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(10): 1148.53; y1+-H(3) O(4) P(9): 1035.45; y1+-H(3) O(4) P(8): 907.39; y1+-H(3) O(4) P(4): 477.25; y1+-H(3) O(4) P(3): 363.20; y1+-H(3) O(4) P(2): 216.13; y2+-H(3) O(4) P(16): 920.90; y2+-H(3) O(4) P(14): 812.86; y2+-H(3) O(4) P(13): 755.35; y2+-H(3) O(4) P(11): 643.30; y1+(2): 314.11","ptmRS..Best.Site.Probabilities":"S17(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(3): 0.0; S(6): 0.0; S(11): 0.0; S(17): 100.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (K4)","Search.ID":"K","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DLEQENVEK","Annotated.Sequence":"[K].DLEQENVEK.[E]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1515,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":552.26407,"Contaminant":"False","Aligned.m.z..Da.":552.26404,"MH...Da.":1103.52087,"Theo..MH...Da.":1103.52151,"DeltaM..ppm.":-0.58,"Deltam.z..Da.":-0.00032,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1567331.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":16.68427,"Ion.Inject.Time..ms.":22,"RT..min.":6.4288,"First.Scan":3473,"Last.Scan":3473,"Master.Scan.s.":3462,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_02.raw","File.ID":"F26","Quan.Info":"","Peptides.Matched":524,"XCorr":0.99,"X..Protein.Groups":1,"Percolator.q.Value":0.00099,"Percolator.PEP":0.00646,"Percolator.SVMScore":0.711,"Precursor.Area":857716.875,"Apex.RT..min.":6.42,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (K4)","Search.ID":"K","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KVIGSFK","Annotated.Sequence":"[K].KVIGSFK.[D]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.3756,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":389.74449,"Contaminant":"False","Aligned.m.z..Da.":389.74447,"MH...Da.":778.48171,"Theo..MH...Da.":778.48215,"DeltaM..ppm.":-0.56,"Deltam.z..Da.":-0.00022,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2963335.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":56.3462,"Ion.Inject.Time..ms.":22,"RT..min.":6.3387,"First.Scan":3336,"Last.Scan":3336,"Master.Scan.s.":3332,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_02.raw","File.ID":"F26","Quan.Info":"","Peptides.Matched":22,"XCorr":2.05,"X..Protein.Groups":1,"Percolator.q.Value":0.00099,"Percolator.PEP":0.00709,"Percolator.SVMScore":0.692,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AB4)","Search.ID":"AB","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ELDEIAASLENL","Annotated.Sequence":"[K].ELDEIAAsLENL.[-]","Modifications":"S8(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6832,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":698.81695,"Contaminant":"False","Aligned.m.z..Da.":698.8171,"MH...Da.":1396.62664,"Theo..MH...Da.":1396.62433,"DeltaM..ppm.":1.65,"Deltam.z..Da.":0.00115,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":399912.5625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":9.79305,"Ion.Inject.Time..ms.":22,"RT..min.":18.2399,"First.Scan":18512,"Last.Scan":18512,"Master.Scan.s.":18502,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_03.raw","File.ID":"F9","Quan.Info":"","Peptides.Matched":837,"XCorr":2.02,"X..Protein.Groups":1,"Percolator.q.Value":0.001,"Percolator.PEP":0.01657,"Percolator.SVMScore":0.422,"ptmRS..Binomial.Peptide.Score":171.78,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"S8(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(8): 100.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (R4)","Search.ID":"R","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"SFLTYIK","Annotated.Sequence":"[K].SFLTYIK.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6815,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":436.24947,"Contaminant":"False","Aligned.m.z..Da.":436.2496,"MH...Da.":871.49166,"Theo..MH...Da.":871.49238,"DeltaM..ppm.":-0.83,"Deltam.z..Da.":-0.00036,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":644574.625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":2.39983,"Ion.Inject.Time..ms.":22,"RT..min.":13.6659,"First.Scan":13033,"Last.Scan":13033,"Master.Scan.s.":13029,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_01.raw","File.ID":"F19","Quan.Info":"","Peptides.Matched":273,"XCorr":1.57,"X..Protein.Groups":1,"Percolator.q.Value":0.00101,"Percolator.PEP":0.01903,"Percolator.SVMScore":0.466,"Precursor.Area":5915187.5,"Apex.RT..min.":13.69,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (V4)","Search.ID":"V","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KVIGSFK","Annotated.Sequence":"[K].KVIGSFK.[D]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.2759,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":389.74486,"Contaminant":"False","Aligned.m.z..Da.":389.74486,"MH...Da.":778.48245,"Theo..MH...Da.":778.48215,"DeltaM..ppm.":0.39,"Deltam.z..Da.":0.00015,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2837024,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":48.37338,"Ion.Inject.Time..ms.":22,"RT..min.":6.21,"First.Scan":3141,"Last.Scan":3141,"Master.Scan.s.":3138,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_03.raw","File.ID":"F15","Quan.Info":"","Peptides.Matched":22,"XCorr":1.74,"X..Protein.Groups":1,"Percolator.q.Value":0.00101,"Percolator.PEP":0.01266,"Percolator.SVMScore":0.445,"Precursor.Area":1673881.5,"Apex.RT..min.":6.21,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (V4)","Search.ID":"V","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"VFDNIQR","Annotated.Sequence":"[R].VFDNIQR.[R]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.4144,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":446.23823,"Contaminant":"False","Aligned.m.z..Da.":446.23832,"MH...Da.":891.46919,"Theo..MH...Da.":891.46829,"DeltaM..ppm.":1.01,"Deltam.z..Da.":0.00045,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":4441392,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":49.24065,"Ion.Inject.Time..ms.":19.896,"RT..min.":8.2535,"First.Scan":6248,"Last.Scan":6248,"Master.Scan.s.":6245,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_03.raw","File.ID":"F15","Quan.Info":"","Peptides.Matched":367,"XCorr":1.81,"X..Protein.Groups":1,"Percolator.q.Value":0.00101,"Percolator.PEP":0.00742,"Percolator.SVMScore":0.543,"Precursor.Area":2799589.5,"Apex.RT..min.":8.25,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (V4)","Search.ID":"V","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ELDEIAASLENL","Annotated.Sequence":"[K].ELDEIAAsLENL.[-]","Modifications":"S8(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":698.81512,"Contaminant":"False","Aligned.m.z..Da.":698.81492,"MH...Da.":1396.62297,"Theo..MH...Da.":1396.62433,"DeltaM..ppm.":-0.98,"Deltam.z..Da.":-0.00068,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":497783.28125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":6.71105,"Ion.Inject.Time..ms.":22,"RT..min.":18.2419,"First.Scan":18797,"Last.Scan":18797,"Master.Scan.s.":18785,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_03.raw","File.ID":"F15","Quan.Info":"","Peptides.Matched":922,"XCorr":2.5,"X..Protein.Groups":1,"Percolator.q.Value":0.00101,"Percolator.PEP":0.01188,"Percolator.SVMScore":0.456,"ptmRS..Binomial.Peptide.Score":194.86,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"S8(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(8): 100.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (H4)","Search.ID":"H","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ELDEIAASLENL","Annotated.Sequence":"[K].ELDEIAASLENL.[-]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.512,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":658.8342,"Contaminant":"False","Aligned.m.z..Da.":658.83404,"MH...Da.":1316.66114,"Theo..MH...Da.":1316.658,"DeltaM..ppm.":2.38,"Deltam.z..Da.":0.00157,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":710185.875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":18.5086,"First.Scan":19381,"Last.Scan":19381,"Master.Scan.s.":19374,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_05.raw","File.ID":"F29","Quan.Info":"","Peptides.Matched":679,"XCorr":2.09,"X..Protein.Groups":1,"Percolator.q.Value":0.00102,"Percolator.PEP":0.01332,"Percolator.SVMScore":0.525,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AH4)","Search.ID":"AH","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ILISTDLLAR","Annotated.Sequence":"[R].ILISTDLLAR.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5317,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":557.84586,"Contaminant":"False","Aligned.m.z..Da.":557.84578,"MH...Da.":1114.68446,"Theo..MH...Da.":1114.68304,"DeltaM..ppm.":1.27,"Deltam.z..Da.":0.00071,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":248369.6875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":25.60565,"Ion.Inject.Time..ms.":22,"RT..min.":15.6343,"First.Scan":15298,"Last.Scan":15298,"Master.Scan.s.":15297,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y25_03.raw","File.ID":"F3","Quan.Info":"","Peptides.Matched":124,"XCorr":1.26,"X..Protein.Groups":1,"Percolator.q.Value":0.00103,"Percolator.PEP":0.00927,"Percolator.SVMScore":0.527,"Precursor.Area":330070.6875,"Apex.RT..min.":15.61,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (U4)","Search.ID":"U","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ELDEIAASLENL","Annotated.Sequence":"[K].ELDEIAASLENL.[-]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5545,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":658.83372,"Contaminant":"False","Aligned.m.z..Da.":658.83395,"MH...Da.":1316.66016,"Theo..MH...Da.":1316.658,"DeltaM..ppm.":1.64,"Deltam.z..Da.":0.00108,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":603831.5625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":18.4611,"First.Scan":19167,"Last.Scan":19167,"Master.Scan.s.":19160,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_04.raw","File.ID":"F16","Quan.Info":"","Peptides.Matched":665,"XCorr":2.02,"X..Protein.Groups":1,"Percolator.q.Value":0.00104,"Percolator.PEP":0.0125,"Percolator.SVMScore":0.546,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (U4)","Search.ID":"U","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LEAGLSDSK","Annotated.Sequence":"[K].LEAGLsDSK.[Q]","Modifications":"S6(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.0758,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":500.22526,"Contaminant":"False","Aligned.m.z..Da.":500.22531,"MH...Da.":999.44325,"Theo..MH...Da.":999.43943,"DeltaM..ppm.":3.82,"Deltam.z..Da.":0.00191,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1634238,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":42.07132,"Ion.Inject.Time..ms.":22,"RT..min.":7.5772,"First.Scan":5395,"Last.Scan":5395,"Master.Scan.s.":5387,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_04.raw","File.ID":"F16","Quan.Info":"","Peptides.Matched":654,"XCorr":2.11,"X..Protein.Groups":1,"Percolator.q.Value":0.00104,"Percolator.PEP":0.01217,"Percolator.SVMScore":0.551,"Precursor.Area":2065493.75,"Apex.RT..min.":7.59,"ptmRS..Binomial.Peptide.Score":217.93,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(8): 788.38; y1+-H(3) O(4) P(7): 659.34; y1+-H(3) O(4) P(6): 588.30; y1+-H(3) O(4) P(5): 531.28; y1+-H(3) O(4) P(4): 418.19; y1+(3): 349.17; y1+(2): 234.14","ptmRS..Best.Site.Probabilities":"S6(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(6): 100.0; S(8): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AD4)","Search.ID":"AD","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"SFLTYIK","Annotated.Sequence":"[K].SFLTYIK.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.625,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":436.24946,"Contaminant":"False","Aligned.m.z..Da.":436.24951,"MH...Da.":871.49164,"Theo..MH...Da.":871.49238,"DeltaM..ppm.":-0.85,"Deltam.z..Da.":-0.00037,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":730569.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":13.6876,"First.Scan":12656,"Last.Scan":12656,"Master.Scan.s.":12651,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_01.raw","File.ID":"F7","Quan.Info":"","Peptides.Matched":273,"XCorr":1.6,"X..Protein.Groups":1,"Percolator.q.Value":0.00105,"Percolator.PEP":0.0063,"Percolator.SVMScore":0.529,"Precursor.Area":2138182.75,"Apex.RT..min.":13.71,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (P4)","Search.ID":"P","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"NHQLEEEIEK","Annotated.Sequence":"[K].NHQLEEEIEK.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.3628,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":634.81061,"Contaminant":"False","Aligned.m.z..Da.":634.81063,"MH...Da.":1268.61395,"Theo..MH...Da.":1268.61172,"DeltaM..ppm.":1.75,"Deltam.z..Da.":0.00111,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":482679.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":82.00385,"Ion.Inject.Time..ms.":22,"RT..min.":7.1874,"First.Scan":4755,"Last.Scan":4755,"Master.Scan.s.":4749,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_03.raw","File.ID":"F21","Quan.Info":"","Peptides.Matched":875,"XCorr":1.13,"X..Protein.Groups":1,"Percolator.q.Value":0.00107,"Percolator.PEP":0.02259,"Percolator.SVMScore":0.411,"Precursor.Area":419278.34375,"Apex.RT..min.":7.18,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (K4)","Search.ID":"K","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"MGSINNNFNTNNNSNTDLDR","Annotated.Sequence":"[-].mGSINNNFNTNNNsNTDLDR.[D]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S14(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P39517","Master.Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","Protein.Accessions":"P39517","Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0493,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":749.31022,"Contaminant":"False","Aligned.m.z..Da.":749.3102,"MH...Da.":2245.91613,"Theo..MH...Da.":2245.91057,"DeltaM..ppm.":2.47,"Deltam.z..Da.":0.00185,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":798120.875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":31.20641,"Ion.Inject.Time..ms.":22,"RT..min.":11.9866,"First.Scan":10941,"Last.Scan":10941,"Master.Scan.s.":10937,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_02.raw","File.ID":"F26","Quan.Info":"","Peptides.Matched":1478,"XCorr":2.23,"X..Protein.Groups":1,"Percolator.q.Value":0.00108,"Percolator.PEP":0.01065,"Percolator.SVMScore":0.607,"Precursor.Area":807432.375,"Apex.RT..min.":11.98,"ptmRS..Binomial.Peptide.Score":222.75,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(11): 1245.55; y1+-H(3) O(4) P(10): 1144.50; y1+-H(3) O(4) P(9): 1030.45; y1+-H(3) O(4) P(8): 916.41; y1+-H(3) O(4) P(7): 802.37; y2+-H(3) O(4) P(7): 401.69; y1+(6): 733.35; y1+(5): 619.30","ptmRS..Best.Site.Probabilities":"S14(Phospho): 99.99","ptmRS..Phospho.Site.Probabilities":"S(3): 0.0; T(10): 0.0; S(14): 100.0; T(16): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (K4)","Search.ID":"K","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].msHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss); S2(Phospho); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.5022,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":980.05426,"Contaminant":"False","Aligned.m.z..Da.":980.05422,"MH...Da.":2938.14825,"Theo..MH...Da.":2938.15509,"DeltaM..ppm.":-2.33,"Deltam.z..Da.":-0.00228,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":346208.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":15.0769,"First.Scan":14922,"Last.Scan":14922,"Master.Scan.s.":14915,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_02.raw","File.ID":"F26","Quan.Info":"","Peptides.Matched":1194,"XCorr":2.31,"X..Protein.Groups":1,"Percolator.q.Value":0.00108,"Percolator.PEP":0.00919,"Percolator.SVMScore":0.638,"ptmRS..Binomial.Peptide.Score":128.09,"ptmRS..Isoform.Confidence.Probability":0.5,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"S2(Phospho): 100; Y12(Phospho): 50; S13(Phospho): 50","ptmRS..Phospho.Site.Probabilities":"S(2): 100.0; Y(12): 50.0; S(13): 50.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (J4)","Search.ID":"J","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FFISNPDLVDRLEK","Annotated.Sequence":"[R].FFISNPDLVDRLEK.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q03558","Master.Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","Protein.Accessions":"Q03558","Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":5,"DeltaScore":0.1795,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":564.97068,"Contaminant":"False","Aligned.m.z..Da.":564.97067,"MH...Da.":1692.89748,"Theo..MH...Da.":1692.89555,"DeltaM..ppm.":1.14,"Deltam.z..Da.":0.00064,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":962692.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":72.75324,"Ion.Inject.Time..ms.":22,"RT..min.":15.3498,"First.Scan":15298,"Last.Scan":15298,"Master.Scan.s.":15294,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_03.raw","File.ID":"F27","Quan.Info":"","Peptides.Matched":589,"XCorr":1.17,"X..Protein.Groups":1,"Percolator.q.Value":0.0011,"Percolator.PEP":0.00728,"Percolator.SVMScore":0.597,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (J4)","Search.ID":"J","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].msEGITDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S2(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":3,"DeltaScore":0.0878,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1096.46537,"Contaminant":"False","Aligned.m.z..Da.":1096.46529,"MH...Da.":2191.92346,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":-2,"Deltam.z..Da.":-0.00219,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":141572.82812,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":90.20793,"Ion.Inject.Time..ms.":22,"RT..min.":15.7008,"First.Scan":15745,"Last.Scan":15745,"Master.Scan.s.":15736,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_03.raw","File.ID":"F27","Quan.Info":"","Peptides.Matched":1848,"XCorr":2.05,"X..Protein.Groups":1,"Percolator.q.Value":0.0011,"Percolator.PEP":0.008,"Percolator.SVMScore":0.575,"Precursor.Area":23399554,"Apex.RT..min.":15.78,"ptmRS..Binomial.Peptide.Score":201.84,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(2): 112.04; b1+-H(3) O(4) P(3): 241.08; b1+-H(3) O(4) P(4): 298.10; b1+-H(3) O(4) P(5): 411.19; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35","ptmRS..Best.Site.Probabilities":"S2(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 100.0; T(6): 0.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (J4)","Search.ID":"J","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"VFDNIQR","Annotated.Sequence":"[R].VFDNIQR.[R]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.3765,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":446.23818,"Contaminant":"False","Aligned.m.z..Da.":446.23821,"MH...Da.":891.46909,"Theo..MH...Da.":891.46829,"DeltaM..ppm.":0.9,"Deltam.z..Da.":0.0004,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":6245467.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":55.67999,"Ion.Inject.Time..ms.":10.781,"RT..min.":8.2703,"First.Scan":6275,"Last.Scan":6275,"Master.Scan.s.":6272,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_03.raw","File.ID":"F27","Quan.Info":"","Peptides.Matched":367,"XCorr":1.7,"X..Protein.Groups":1,"Percolator.q.Value":0.0011,"Percolator.PEP":0.00729,"Percolator.SVMScore":0.597,"Precursor.Area":5487739,"Apex.RT..min.":8.26,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (L4)","Search.ID":"L","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].msEGITDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S2(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":3,"DeltaScore":0.1449,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1096.46314,"Contaminant":"False","Aligned.m.z..Da.":1096.46297,"MH...Da.":2191.91899,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":-4.04,"Deltam.z..Da.":-0.00443,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":342583.96875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":85.90472,"Ion.Inject.Time..ms.":22,"RT..min.":16.0658,"First.Scan":15982,"Last.Scan":15982,"Master.Scan.s.":15980,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_01.raw","File.ID":"F25","Quan.Info":"","Peptides.Matched":1923,"XCorr":2.07,"X..Protein.Groups":1,"Percolator.q.Value":0.0011,"Percolator.PEP":0.01334,"Percolator.SVMScore":0.604,"Precursor.Area":16847080,"Apex.RT..min.":15.79,"ptmRS..Binomial.Peptide.Score":244.15,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(2): 112.04; b1+-H(3) O(4) P(3): 241.08; b1+-H(3) O(4) P(4): 298.10; b1+-H(3) O(4) P(5): 411.19; b1+-H(3) O(4) P(6): 512.24; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+-H(3) O(4) P(9): 869.39","ptmRS..Best.Site.Probabilities":"S2(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 100.0; T(6): 0.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSSPLHRLSPTGK","Annotated.Sequence":"[R].MSsPLHRLsPTGK.[S]","Modifications":"S3(Phospho); S9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.4764,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":785.8456,"Contaminant":"False","Aligned.m.z..Da.":785.84566,"MH...Da.":1570.68393,"Theo..MH...Da.":1570.68486,"DeltaM..ppm.":-0.59,"Deltam.z..Da.":-0.00046,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3781071,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":89.84572,"Ion.Inject.Time..ms.":13.681,"RT..min.":8.6739,"First.Scan":6762,"Last.Scan":6762,"Master.Scan.s.":6754,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":1035,"XCorr":2.12,"X..Protein.Groups":1,"Percolator.q.Value":0.0011,"Percolator.PEP":0.0082,"Percolator.SVMScore":0.594,"ptmRS..Binomial.Peptide.Score":231.06,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(9): 1071.48; y2+-H(3) O(4) P(11): 627.82; y2+- -2H(3) O(4) P -2H(3) O(4) P(11): 578.83","ptmRS..Best.Site.Probabilities":"S3(Phospho): 99.99; S9(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(3): 100.0; S(9): 100.0; T(11): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ELDEIAASLENL","Annotated.Sequence":"[K].ELDEIAASLENL.[-]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6042,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":658.83248,"Contaminant":"False","Aligned.m.z..Da.":658.83256,"MH...Da.":1316.65769,"Theo..MH...Da.":1316.658,"DeltaM..ppm.":-0.24,"Deltam.z..Da.":-0.00016,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2490498.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":18.4618,"First.Scan":19380,"Last.Scan":19380,"Master.Scan.s.":19377,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":762,"XCorr":2.88,"X..Protein.Groups":1,"Percolator.q.Value":0.0011,"Percolator.PEP":0.0079,"Percolator.SVMScore":0.602,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (G4)","Search.ID":"G","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ILISTDLLAR","Annotated.Sequence":"[R].ILISTDLLAR.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5309,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":557.84614,"Contaminant":"False","Aligned.m.z..Da.":557.84617,"MH...Da.":1114.685,"Theo..MH...Da.":1114.68304,"DeltaM..ppm.":1.77,"Deltam.z..Da.":0.00098,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":394875.78125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":20.72688,"Ion.Inject.Time..ms.":22,"RT..min.":15.691,"First.Scan":16417,"Last.Scan":16417,"Master.Scan.s.":16416,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_06.raw","File.ID":"F30","Quan.Info":"","Peptides.Matched":124,"XCorr":1.62,"X..Protein.Groups":1,"Percolator.q.Value":0.00111,"Percolator.PEP":0.01032,"Percolator.SVMScore":0.623,"Precursor.Area":4150825.5,"Apex.RT..min.":15.64,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (H4)","Search.ID":"H","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DFLLKPELSR","Annotated.Sequence":"[K].DFLLKPELSR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7113,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":609.34795,"Contaminant":"False","Aligned.m.z..Da.":609.34803,"MH...Da.":1217.68862,"Theo..MH...Da.":1217.68885,"DeltaM..ppm.":-0.19,"Deltam.z..Da.":-0.00011,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":807374.3125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":10.8312,"Ion.Inject.Time..ms.":22,"RT..min.":13.2254,"First.Scan":13330,"Last.Scan":13330,"Master.Scan.s.":13323,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_05.raw","File.ID":"F29","Quan.Info":"","Peptides.Matched":265,"XCorr":1.94,"X..Protein.Groups":1,"Percolator.q.Value":0.00113,"Percolator.PEP":0.01437,"Percolator.SVMScore":0.511,"Precursor.Area":783808.6875,"Apex.RT..min.":13.24,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (U4)","Search.ID":"U","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"VFDNIQR","Annotated.Sequence":"[R].VFDNIQR.[R]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.3908,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":446.23822,"Contaminant":"False","Aligned.m.z..Da.":446.2383,"MH...Da.":891.46916,"Theo..MH...Da.":891.46829,"DeltaM..ppm.":0.97,"Deltam.z..Da.":0.00043,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":5356305.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":40.68816,"Ion.Inject.Time..ms.":17.066,"RT..min.":8.2598,"First.Scan":6283,"Last.Scan":6283,"Master.Scan.s.":6271,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_04.raw","File.ID":"F16","Quan.Info":"","Peptides.Matched":367,"XCorr":1.74,"X..Protein.Groups":1,"Percolator.q.Value":0.00114,"Percolator.PEP":0.01678,"Percolator.SVMScore":0.488,"Precursor.Area":3254482.25,"Apex.RT..min.":8.26,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Z4)","Search.ID":"Z","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"VFDNIQR","Annotated.Sequence":"[R].VFDNIQR.[R]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.372,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":446.23809,"Contaminant":"False","Aligned.m.z..Da.":446.23813,"MH...Da.":891.46889,"Theo..MH...Da.":891.46829,"DeltaM..ppm.":0.68,"Deltam.z..Da.":0.0003,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2580006.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":63.78694,"Ion.Inject.Time..ms.":22,"RT..min.":8.2539,"First.Scan":6240,"Last.Scan":6240,"Master.Scan.s.":6236,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_05.raw","File.ID":"F11","Quan.Info":"","Peptides.Matched":367,"XCorr":1.64,"X..Protein.Groups":1,"Percolator.q.Value":0.00114,"Percolator.PEP":0.02528,"Percolator.SVMScore":0.364,"Precursor.Area":1566128.625,"Apex.RT..min.":8.26,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (W4)","Search.ID":"W","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KVIGSFK","Annotated.Sequence":"[K].KVIGSFK.[D]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.3367,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":389.74477,"Contaminant":"False","Aligned.m.z..Da.":389.74472,"MH...Da.":778.48226,"Theo..MH...Da.":778.48215,"DeltaM..ppm.":0.14,"Deltam.z..Da.":0.00006,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2146271.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":45.3777,"Ion.Inject.Time..ms.":22,"RT..min.":6.193,"First.Scan":3167,"Last.Scan":3167,"Master.Scan.s.":3160,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_02.raw","File.ID":"F14","Quan.Info":"","Peptides.Matched":22,"XCorr":1.99,"X..Protein.Groups":1,"Percolator.q.Value":0.00116,"Percolator.PEP":0.01398,"Percolator.SVMScore":0.534,"Precursor.Area":1857982.5,"Apex.RT..min.":6.2,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Y4)","Search.ID":"Y","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LEAGLSDSK","Annotated.Sequence":"[K].LEAGLsDSK.[Q]","Modifications":"S6(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1526,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":500.22345,"Contaminant":"False","Aligned.m.z..Da.":500.22344,"MH...Da.":999.43964,"Theo..MH...Da.":999.43943,"DeltaM..ppm.":0.2,"Deltam.z..Da.":0.0001,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1660175.875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":29.48348,"Ion.Inject.Time..ms.":22,"RT..min.":7.7074,"First.Scan":5861,"Last.Scan":5861,"Master.Scan.s.":5849,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_06.raw","File.ID":"F12","Quan.Info":"","Peptides.Matched":652,"XCorr":1.9,"X..Protein.Groups":1,"Percolator.q.Value":0.00116,"Percolator.PEP":0.01162,"Percolator.SVMScore":0.579,"Precursor.Area":1092160.375,"Apex.RT..min.":7.7,"ptmRS..Binomial.Peptide.Score":192.53,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(8): 788.38; y1+-H(3) O(4) P(7): 659.34; y1+-H(3) O(4) P(6): 588.30; y1+-H(3) O(4) P(5): 531.28; y1+-H(3) O(4) P(4): 418.19; y1+(3): 349.17; y1+(2): 234.14","ptmRS..Best.Site.Probabilities":"S6(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(6): 100.0; S(8): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AI4)","Search.ID":"AI","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ELDEIAASLENL","Annotated.Sequence":"[K].ELDEIAAsLENL.[-]","Modifications":"S8(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6786,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":698.81599,"Contaminant":"False","Aligned.m.z..Da.":698.81576,"MH...Da.":1396.62471,"Theo..MH...Da.":1396.62433,"DeltaM..ppm.":0.27,"Deltam.z..Da.":0.00019,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":522064.9375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":6.57452,"Ion.Inject.Time..ms.":22,"RT..min.":18.248,"First.Scan":18092,"Last.Scan":18092,"Master.Scan.s.":18087,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y25_02.raw","File.ID":"F2","Quan.Info":"","Peptides.Matched":882,"XCorr":1.96,"X..Protein.Groups":1,"Percolator.q.Value":0.00118,"Percolator.PEP":0.02433,"Percolator.SVMScore":0.429,"ptmRS..Binomial.Peptide.Score":183.22,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"S8(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(8): 100.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (J4)","Search.ID":"J","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ELDEIAASLENL","Annotated.Sequence":"[K].ELDEIAAsLENL.[-]","Modifications":"S8(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6782,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":698.81667,"Contaminant":"False","Aligned.m.z..Da.":698.81591,"MH...Da.":1396.62606,"Theo..MH...Da.":1396.62433,"DeltaM..ppm.":1.24,"Deltam.z..Da.":0.00086,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":650627.8125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":18.2348,"First.Scan":18992,"Last.Scan":18992,"Master.Scan.s.":18985,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_03.raw","File.ID":"F27","Quan.Info":"","Peptides.Matched":809,"XCorr":2.61,"X..Protein.Groups":1,"Percolator.q.Value":0.00118,"Percolator.PEP":0.01129,"Percolator.SVMScore":0.502,"ptmRS..Binomial.Peptide.Score":197.11,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"S8(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(8): 100.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (J4)","Search.ID":"J","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ELDEIAASLENL","Annotated.Sequence":"[K].ELDEIAASLENL.[-]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.4677,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":658.83247,"Contaminant":"False","Aligned.m.z..Da.":658.83174,"MH...Da.":1316.65766,"Theo..MH...Da.":1316.658,"DeltaM..ppm.":-0.26,"Deltam.z..Da.":-0.00017,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":854367.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":34.40699,"Ion.Inject.Time..ms.":22,"RT..min.":18.4518,"First.Scan":19275,"Last.Scan":19275,"Master.Scan.s.":19271,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_03.raw","File.ID":"F27","Quan.Info":"","Peptides.Matched":760,"XCorr":1.86,"X..Protein.Groups":1,"Percolator.q.Value":0.00118,"Percolator.PEP":0.01048,"Percolator.SVMScore":0.517,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (I4)","Search.ID":"I","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"SFLTYIK","Annotated.Sequence":"[K].SFLTYIK.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5895,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":436.24986,"Contaminant":"False","Aligned.m.z..Da.":436.24985,"MH...Da.":871.49245,"Theo..MH...Da.":871.49238,"DeltaM..ppm.":0.07,"Deltam.z..Da.":0.00003,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2328108.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":2.80528,"Ion.Inject.Time..ms.":22,"RT..min.":13.6717,"First.Scan":13195,"Last.Scan":13195,"Master.Scan.s.":13191,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_04.raw","File.ID":"F28","Quan.Info":"","Peptides.Matched":273,"XCorr":1.9,"X..Protein.Groups":1,"Percolator.q.Value":0.00118,"Percolator.PEP":0.0127,"Percolator.SVMScore":0.5,"Precursor.Area":9171451,"Apex.RT..min.":13.7,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (I4)","Search.ID":"I","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"VFDNIQR","Annotated.Sequence":"[R].VFDNIQR.[R]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.4171,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":446.2385,"Contaminant":"False","Aligned.m.z..Da.":446.23855,"MH...Da.":891.46972,"Theo..MH...Da.":891.46829,"DeltaM..ppm.":1.6,"Deltam.z..Da.":0.00071,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":4364764.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":39.16419,"Ion.Inject.Time..ms.":17.118,"RT..min.":8.2512,"First.Scan":6256,"Last.Scan":6256,"Master.Scan.s.":6249,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_04.raw","File.ID":"F28","Quan.Info":"","Peptides.Matched":362,"XCorr":2.11,"X..Protein.Groups":1,"Percolator.q.Value":0.00118,"Percolator.PEP":0.01298,"Percolator.SVMScore":0.496,"Precursor.Area":5916488,"Apex.RT..min.":8.26,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (K4)","Search.ID":"K","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"VFDNIQR","Annotated.Sequence":"[R].VFDNIQR.[R]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.3931,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":446.23799,"Contaminant":"False","Aligned.m.z..Da.":446.23807,"MH...Da.":891.46869,"Theo..MH...Da.":891.46829,"DeltaM..ppm.":0.45,"Deltam.z..Da.":0.0002,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":8742105,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":45.823,"Ion.Inject.Time..ms.":9.4,"RT..min.":8.2685,"First.Scan":6273,"Last.Scan":6273,"Master.Scan.s.":6270,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_02.raw","File.ID":"F26","Quan.Info":"","Peptides.Matched":404,"XCorr":1.73,"X..Protein.Groups":1,"Percolator.q.Value":0.00118,"Percolator.PEP":0.01148,"Percolator.SVMScore":0.592,"Precursor.Area":5509500,"Apex.RT..min.":8.26,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AC4)","Search.ID":"AC","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ELDEIAASLENL","Annotated.Sequence":"[K].ELDEIAAsLENL.[-]","Modifications":"S8(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.625,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":698.81626,"Contaminant":"False","Aligned.m.z..Da.":698.81649,"MH...Da.":1396.62525,"Theo..MH...Da.":1396.62433,"DeltaM..ppm.":0.66,"Deltam.z..Da.":0.00046,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":623870.6875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":3.04648,"Ion.Inject.Time..ms.":22,"RT..min.":18.2431,"First.Scan":18427,"Last.Scan":18427,"Master.Scan.s.":18424,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_02.raw","File.ID":"F8","Quan.Info":"","Peptides.Matched":850,"XCorr":2,"X..Protein.Groups":1,"Percolator.q.Value":0.0012,"Percolator.PEP":0.02288,"Percolator.SVMScore":0.343,"Precursor.Area":480098.0625,"Apex.RT..min.":18.25,"ptmRS..Binomial.Peptide.Score":158.65,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"S8(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(8): 100.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ELAYQIR","Annotated.Sequence":"[R].ELAYQIR.[N]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.3056,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":446.74751,"Contaminant":"False","Aligned.m.z..Da.":446.74754,"MH...Da.":892.48775,"Theo..MH...Da.":892.48869,"DeltaM..ppm.":-1.05,"Deltam.z..Da.":-0.00047,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3121157,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":84.82291,"Ion.Inject.Time..ms.":10.225,"RT..min.":9.5496,"First.Scan":7959,"Last.Scan":7959,"Master.Scan.s.":7950,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":379,"XCorr":1.44,"X..Protein.Groups":1,"Percolator.q.Value":0.0012,"Percolator.PEP":0.01077,"Percolator.SVMScore":0.534,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"IQDPQMTGYVSTR","Annotated.Sequence":"[R].IQDPQmtGYVsTR.[Y]","Modifications":"M6(Oxidation); T7(Phospho); S11(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P32485","Master.Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","Protein.Accessions":"P32485","Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.0476,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":836.3271,"Contaminant":"False","Aligned.m.z..Da.":836.32713,"MH...Da.":1671.64692,"Theo..MH...Da.":1671.64853,"DeltaM..ppm.":-0.96,"Deltam.z..Da.":-0.0008,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":4231682,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":54.68397,"Ion.Inject.Time..ms.":22,"RT..min.":7.6441,"First.Scan":5260,"Last.Scan":5260,"Master.Scan.s.":5259,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":732,"XCorr":1.89,"X..Protein.Groups":1,"Percolator.q.Value":0.0012,"Percolator.PEP":0.01067,"Percolator.SVMScore":0.536,"Precursor.Area":7466476.5,"Apex.RT..min.":7.68,"ptmRS..Binomial.Peptide.Score":316.69,"ptmRS..Isoform.Confidence.Probability":0.006,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(11): 1332.53; y1+-H(3) O(4) P(10): 1217.50; y1+-H(3) O(4) P(8): 992.39; y1+-H(3) O(4) P(7): 845.36; y2+-H(3) O(4) P(11): 666.77; y2+-H(3) O(4) P(10): 609.25; y1+(4): 462.27; y1+(3): 363.20","ptmRS..Best.Site.Probabilities":"T7(Phospho): 100; Y9(Phospho): 99.38","ptmRS..Phospho.Site.Probabilities":"T(7): 100.0; Y(9): 99.4; S(11): 0.6; T(12): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"IGNNELLHR","Annotated.Sequence":"[K].IGNNELLHR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q03558","Master.Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","Protein.Accessions":"Q03558","Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.3737,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":533.29426,"Contaminant":"False","Aligned.m.z..Da.":533.29428,"MH...Da.":1065.58125,"Theo..MH...Da.":1065.57997,"DeltaM..ppm.":1.21,"Deltam.z..Da.":0.00064,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":287372.0625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":88.74403,"Ion.Inject.Time..ms.":22,"RT..min.":7.5886,"First.Scan":5183,"Last.Scan":5183,"Master.Scan.s.":5181,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":285,"XCorr":0.99,"X..Protein.Groups":1,"Percolator.q.Value":0.0012,"Percolator.PEP":0.01055,"Percolator.SVMScore":0.539,"Precursor.Area":1985071.25,"Apex.RT..min.":7.61,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ELDEIAASLENL","Annotated.Sequence":"[K].ELDEIAAsLENL.[-]","Modifications":"S8(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6909,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":698.8157,"Contaminant":"False","Aligned.m.z..Da.":698.8157,"MH...Da.":1396.62412,"Theo..MH...Da.":1396.62433,"DeltaM..ppm.":-0.16,"Deltam.z..Da.":-0.00011,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1514156.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":8.70555,"Ion.Inject.Time..ms.":22,"RT..min.":18.2336,"First.Scan":19144,"Last.Scan":19144,"Master.Scan.s.":19138,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":929,"XCorr":2.75,"X..Protein.Groups":1,"Percolator.q.Value":0.00121,"Percolator.PEP":0.014,"Percolator.SVMScore":0.461,"ptmRS..Binomial.Peptide.Score":218.37,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"S8(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(8): 100.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (T4)","Search.ID":"T","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"IGNNELLHR","Annotated.Sequence":"[K].IGNNELLHR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q03558","Master.Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","Protein.Accessions":"Q03558","Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.2266,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":355.86474,"Contaminant":"False","Aligned.m.z..Da.":355.86478,"MH...Da.":1065.57967,"Theo..MH...Da.":1065.57997,"DeltaM..ppm.":-0.28,"Deltam.z..Da.":-0.0001,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":984089.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":19.21038,"Ion.Inject.Time..ms.":22,"RT..min.":7.663,"First.Scan":5718,"Last.Scan":5718,"Master.Scan.s.":5708,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_05.raw","File.ID":"F17","Quan.Info":"","Peptides.Matched":300,"XCorr":1.28,"X..Protein.Groups":1,"Percolator.q.Value":0.00123,"Percolator.PEP":0.0182,"Percolator.SVMScore":0.425,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (P4)","Search.ID":"P","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ELDEIAASLENL","Annotated.Sequence":"[K].ELDEIAASLENL.[-]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5132,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":658.83331,"Contaminant":"False","Aligned.m.z..Da.":658.8333,"MH...Da.":1316.65935,"Theo..MH...Da.":1316.658,"DeltaM..ppm.":1.02,"Deltam.z..Da.":0.00067,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1185802,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":16.35497,"Ion.Inject.Time..ms.":22,"RT..min.":18.4593,"First.Scan":19219,"Last.Scan":19219,"Master.Scan.s.":19215,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_03.raw","File.ID":"F21","Quan.Info":"","Peptides.Matched":663,"XCorr":1.89,"X..Protein.Groups":1,"Percolator.q.Value":0.00126,"Percolator.PEP":0.02768,"Percolator.SVMScore":0.373,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (I4)","Search.ID":"I","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ILISTDLLAR","Annotated.Sequence":"[R].ILISTDLLAR.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.568,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":557.84551,"Contaminant":"False","Aligned.m.z..Da.":557.84552,"MH...Da.":1114.68375,"Theo..MH...Da.":1114.68304,"DeltaM..ppm.":0.64,"Deltam.z..Da.":0.00036,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":373847.53125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":15.49938,"Ion.Inject.Time..ms.":22,"RT..min.":15.6456,"First.Scan":15688,"Last.Scan":15688,"Master.Scan.s.":15687,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_04.raw","File.ID":"F28","Quan.Info":"","Peptides.Matched":124,"XCorr":1.69,"X..Protein.Groups":1,"Percolator.q.Value":0.00128,"Percolator.PEP":0.01508,"Percolator.SVMScore":0.467,"Precursor.Area":2701803.75,"Apex.RT..min.":15.6,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (J4)","Search.ID":"J","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ELAYQIR","Annotated.Sequence":"[R].ELAYQIR.[N]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.2549,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":446.74804,"Contaminant":"False","Aligned.m.z..Da.":446.74804,"MH...Da.":892.4888,"Theo..MH...Da.":892.48869,"DeltaM..ppm.":0.12,"Deltam.z..Da.":0.00005,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1790753.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":82.10958,"Ion.Inject.Time..ms.":22,"RT..min.":9.5466,"First.Scan":7740,"Last.Scan":7740,"Master.Scan.s.":7728,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_03.raw","File.ID":"F27","Quan.Info":"","Peptides.Matched":402,"XCorr":1.02,"X..Protein.Groups":1,"Percolator.q.Value":0.00128,"Percolator.PEP":0.01211,"Percolator.SVMScore":0.488,"Precursor.Area":568445.25,"Apex.RT..min.":9.95,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"SFLTYIK","Annotated.Sequence":"[K].SFLTYIK.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.638,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":436.24938,"Contaminant":"False","Aligned.m.z..Da.":436.24947,"MH...Da.":871.49149,"Theo..MH...Da.":871.49238,"DeltaM..ppm.":-1.02,"Deltam.z..Da.":-0.00044,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":888194.375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":7.76896,"Ion.Inject.Time..ms.":22,"RT..min.":13.711,"First.Scan":13276,"Last.Scan":13276,"Master.Scan.s.":13267,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":269,"XCorr":1.63,"X..Protein.Groups":1,"Percolator.q.Value":0.00128,"Percolator.PEP":0.01368,"Percolator.SVMScore":0.484,"Precursor.Area":15491181,"Apex.RT..min.":13.75,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (S4)","Search.ID":"S","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LQQTAFDKK","Annotated.Sequence":"[R].LQQTAFDKK.[S]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.2639,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":360.2015,"Contaminant":"False","Aligned.m.z..Da.":360.20153,"MH...Da.":1078.58997,"Theo..MH...Da.":1078.58914,"DeltaM..ppm.":0.77,"Deltam.z..Da.":0.00028,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2748722.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":23.15493,"Ion.Inject.Time..ms.":22,"RT..min.":5.5207,"First.Scan":2574,"Last.Scan":2574,"Master.Scan.s.":2563,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_06.raw","File.ID":"F18","Quan.Info":"","Peptides.Matched":392,"XCorr":1.44,"X..Protein.Groups":1,"Percolator.q.Value":0.00129,"Percolator.PEP":0.02026,"Percolator.SVMScore":0.402,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (G4)","Search.ID":"G","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"SFLTYIK","Annotated.Sequence":"[K].SFLTYIK.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6375,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":436.24997,"Contaminant":"False","Aligned.m.z..Da.":436.24994,"MH...Da.":871.49267,"Theo..MH...Da.":871.49238,"DeltaM..ppm.":0.33,"Deltam.z..Da.":0.00014,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1388084.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":13.7547,"First.Scan":13920,"Last.Scan":13920,"Master.Scan.s.":13917,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_06.raw","File.ID":"F30","Quan.Info":"","Peptides.Matched":109,"XCorr":1.6,"X..Protein.Groups":1,"Percolator.q.Value":0.00129,"Percolator.PEP":0.01342,"Percolator.SVMScore":0.563,"Precursor.Area":8685489,"Apex.RT..min.":13.79,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Q4)","Search.ID":"Q","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"VFDNIQR","Annotated.Sequence":"[R].VFDNIQR.[R]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.4211,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":446.23851,"Contaminant":"False","Aligned.m.z..Da.":446.23859,"MH...Da.":891.46975,"Theo..MH...Da.":891.46829,"DeltaM..ppm.":1.64,"Deltam.z..Da.":0.00073,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":6397234.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":39.93946,"Ion.Inject.Time..ms.":15.441,"RT..min.":8.2654,"First.Scan":6264,"Last.Scan":6264,"Master.Scan.s.":6258,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_02.raw","File.ID":"F20","Quan.Info":"","Peptides.Matched":362,"XCorr":1.9,"X..Protein.Groups":1,"Percolator.q.Value":0.00133,"Percolator.PEP":0.01747,"Percolator.SVMScore":0.534,"Precursor.Area":3863509.75,"Apex.RT..min.":8.26,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AD4)","Search.ID":"AD","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"HGIVEEK","Annotated.Sequence":"[K].HGIVEEK.[I]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.3801,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":406.21831,"Contaminant":"False","Aligned.m.z..Da.":406.21822,"MH...Da.":811.42934,"Theo..MH...Da.":811.43084,"DeltaM..ppm.":-1.86,"Deltam.z..Da.":-0.00075,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1245966.875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":71.55526,"Ion.Inject.Time..ms.":22,"RT..min.":4.4679,"First.Scan":1256,"Last.Scan":1256,"Master.Scan.s.":1254,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_01.raw","File.ID":"F7","Quan.Info":"","Peptides.Matched":181,"XCorr":1.71,"X..Protein.Groups":1,"Percolator.q.Value":0.00136,"Percolator.PEP":0.02348,"Percolator.SVMScore":0.304,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (K4)","Search.ID":"K","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"SFLTYIK","Annotated.Sequence":"[K].SFLTYIK.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6402,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":436.24955,"Contaminant":"False","Aligned.m.z..Da.":436.24963,"MH...Da.":871.49183,"Theo..MH...Da.":871.49238,"DeltaM..ppm.":-0.63,"Deltam.z..Da.":-0.00028,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1257160.875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":13.6728,"First.Scan":13150,"Last.Scan":13150,"Master.Scan.s.":13147,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_02.raw","File.ID":"F26","Quan.Info":"","Peptides.Matched":273,"XCorr":1.64,"X..Protein.Groups":1,"Percolator.q.Value":0.00136,"Percolator.PEP":0.01604,"Percolator.SVMScore":0.521,"Precursor.Area":8468944,"Apex.RT..min.":13.7,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (K4)","Search.ID":"K","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LEAESWQEK","Annotated.Sequence":"[K].LEAESWQEK.[Y]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":3,"DeltaScore":0.1544,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":560.27059,"Contaminant":"False","Aligned.m.z..Da.":560.27069,"MH...Da.":1119.53389,"Theo..MH...Da.":1119.53168,"DeltaM..ppm.":1.98,"Deltam.z..Da.":0.00111,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":481215.125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":94.96507,"Ion.Inject.Time..ms.":19.992,"RT..min.":8.3666,"First.Scan":6391,"Last.Scan":6391,"Master.Scan.s.":6387,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_02.raw","File.ID":"F26","Quan.Info":"","Peptides.Matched":520,"XCorr":1.36,"X..Protein.Groups":1,"Percolator.q.Value":0.00136,"Percolator.PEP":0.01392,"Percolator.SVMScore":0.551,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (G4)","Search.ID":"G","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ELDEIAASLENL","Annotated.Sequence":"[K].ELDEIAAsLENL.[-]","Modifications":"S8(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6481,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":698.8149,"Contaminant":"False","Aligned.m.z..Da.":698.81553,"MH...Da.":1396.62252,"Theo..MH...Da.":1396.62433,"DeltaM..ppm.":-1.3,"Deltam.z..Da.":-0.00091,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":854191.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":29.36,"Ion.Inject.Time..ms.":22,"RT..min.":18.3413,"First.Scan":19297,"Last.Scan":19297,"Master.Scan.s.":19289,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_06.raw","File.ID":"F30","Quan.Info":"","Peptides.Matched":899,"XCorr":2.16,"X..Protein.Groups":1,"Percolator.q.Value":0.00137,"Percolator.PEP":0.01673,"Percolator.SVMScore":0.514,"Precursor.Area":1181619,"Apex.RT..min.":18.35,"ptmRS..Binomial.Peptide.Score":203.96,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"S8(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(8): 100.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (G4)","Search.ID":"G","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ELDEIAASLENL","Annotated.Sequence":"[K].ELDEIAASLENL.[-]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.485,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":658.83221,"Contaminant":"False","Aligned.m.z..Da.":658.8328,"MH...Da.":1316.65714,"Theo..MH...Da.":1316.658,"DeltaM..ppm.":-0.66,"Deltam.z..Da.":-0.00043,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":745397.3125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":26.66698,"Ion.Inject.Time..ms.":22,"RT..min.":18.5104,"First.Scan":19516,"Last.Scan":19516,"Master.Scan.s.":19510,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_06.raw","File.ID":"F30","Quan.Info":"","Peptides.Matched":769,"XCorr":1.67,"X..Protein.Groups":1,"Percolator.q.Value":0.00137,"Percolator.PEP":0.0197,"Percolator.SVMScore":0.479,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (O4)","Search.ID":"O","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KVIGSFK","Annotated.Sequence":"[K].KVIGSFK.[D]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.2335,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":389.74441,"Contaminant":"False","Aligned.m.z..Da.":389.74439,"MH...Da.":778.48155,"Theo..MH...Da.":778.48215,"DeltaM..ppm.":-0.77,"Deltam.z..Da.":-0.0003,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1496925.875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":67.49352,"Ion.Inject.Time..ms.":22,"RT..min.":6.2732,"First.Scan":3317,"Last.Scan":3317,"Master.Scan.s.":3306,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_04.raw","File.ID":"F22","Quan.Info":"","Peptides.Matched":22,"XCorr":1.97,"X..Protein.Groups":1,"Percolator.q.Value":0.00139,"Percolator.PEP":0.022,"Percolator.SVMScore":0.423,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (U4)","Search.ID":"U","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"VFDNIQR","Annotated.Sequence":"[R].VFDNIQR.[R]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.4,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":446.23801,"Contaminant":"False","Aligned.m.z..Da.":446.23809,"MH...Da.":891.46874,"Theo..MH...Da.":891.46829,"DeltaM..ppm.":0.5,"Deltam.z..Da.":0.00022,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1726318.875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":53.38215,"Ion.Inject.Time..ms.":22,"RT..min.":8.2461,"First.Scan":6266,"Last.Scan":6266,"Master.Scan.s.":6258,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_04.raw","File.ID":"F16","Quan.Info":"","Peptides.Matched":367,"XCorr":1.75,"X..Protein.Groups":1,"Percolator.q.Value":0.00141,"Percolator.PEP":0.02686,"Percolator.SVMScore":0.396,"Precursor.Area":3254482.25,"Apex.RT..min.":8.26,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (N4)","Search.ID":"N","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KVIGSFK","Annotated.Sequence":"[K].KVIGSFK.[D]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.2865,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":389.74391,"Contaminant":"False","Aligned.m.z..Da.":389.74395,"MH...Da.":778.48056,"Theo..MH...Da.":778.48215,"DeltaM..ppm.":-2.05,"Deltam.z..Da.":-0.0008,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1513671.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":62.13495,"Ion.Inject.Time..ms.":22,"RT..min.":6.2919,"First.Scan":3936,"Last.Scan":3936,"Master.Scan.s.":3929,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_05.raw","File.ID":"F23","Quan.Info":"","Peptides.Matched":22,"XCorr":1.85,"X..Protein.Groups":1,"Percolator.q.Value":0.00144,"Percolator.PEP":0.02876,"Percolator.SVMScore":0.371,"Precursor.Area":3064859,"Apex.RT..min.":6.32,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FAYSIWK","Annotated.Sequence":"[K].FAYSIWK.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q03558","Master.Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","Protein.Accessions":"Q03558","Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5912,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":457.74249,"Contaminant":"False","Aligned.m.z..Da.":457.74249,"MH...Da.":914.47771,"Theo..MH...Da.":914.47707,"DeltaM..ppm.":0.7,"Deltam.z..Da.":0.00032,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1264182.375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":36.23423,"Ion.Inject.Time..ms.":22,"RT..min.":13.8264,"First.Scan":13478,"Last.Scan":13478,"Master.Scan.s.":13470,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":227,"XCorr":1.59,"X..Protein.Groups":1,"Percolator.q.Value":0.00145,"Percolator.PEP":0.01828,"Percolator.SVMScore":0.41,"Precursor.Area":662610.25,"Apex.RT..min.":13.82,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (K4)","Search.ID":"K","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"VFDNIQR","Annotated.Sequence":"[R].VFDNIQR.[R]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.4469,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":446.23832,"Contaminant":"False","Aligned.m.z..Da.":446.23841,"MH...Da.":891.46937,"Theo..MH...Da.":891.46829,"DeltaM..ppm.":1.21,"Deltam.z..Da.":0.00054,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":5131507,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":35.92955,"Ion.Inject.Time..ms.":16.302,"RT..min.":8.2483,"First.Scan":6249,"Last.Scan":6249,"Master.Scan.s.":6244,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_02.raw","File.ID":"F26","Quan.Info":"","Peptides.Matched":368,"XCorr":2.26,"X..Protein.Groups":1,"Percolator.q.Value":0.00146,"Percolator.PEP":0.01744,"Percolator.SVMScore":0.504,"Precursor.Area":5509500,"Apex.RT..min.":8.26,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"TALNIPK","Annotated.Sequence":"[K].TALNIPK.[K]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P39517","Master.Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","Protein.Accessions":"P39517","Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":3,"DeltaScore":0.2549,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":378.73433,"Contaminant":"False","Aligned.m.z..Da.":378.73436,"MH...Da.":756.46139,"Theo..MH...Da.":756.46142,"DeltaM..ppm.":-0.04,"Deltam.z..Da.":-1e-05,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1700422.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":76.7291,"Ion.Inject.Time..ms.":22,"RT..min.":9.0357,"First.Scan":7269,"Last.Scan":7269,"Master.Scan.s.":7261,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":94,"XCorr":1.02,"X..Protein.Groups":1,"Percolator.q.Value":0.00146,"Percolator.PEP":0.02057,"Percolator.SVMScore":0.404,"Precursor.Area":1257567.75,"Apex.RT..min.":9.04,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ILSSSFHN","Annotated.Sequence":"[K].ILSSsFHN.[-]","Modifications":"S5(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1359,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":492.71329,"Contaminant":"False","Aligned.m.z..Da.":492.71331,"MH...Da.":984.4193,"Theo..MH...Da.":984.41864,"DeltaM..ppm.":0.67,"Deltam.z..Da.":0.00033,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3833946.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":9.83952,"Ion.Inject.Time..ms.":22,"RT..min.":9.2741,"First.Scan":7593,"Last.Scan":7593,"Master.Scan.s.":7586,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":312,"XCorr":1.84,"X..Protein.Groups":1,"Percolator.q.Value":0.00146,"Percolator.PEP":0.0202,"Percolator.SVMScore":0.407,"Precursor.Area":2323155.25,"Apex.RT..min.":9.27,"ptmRS..Binomial.Peptide.Score":198.98,"ptmRS..Isoform.Confidence.Probability":0.996,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(7): 773.36; y1+-H(3) O(4) P(6): 660.27; y1+-H(3) O(4) P(5): 573.24; y1+-H(3) O(4) P(4): 486.21","ptmRS..Best.Site.Probabilities":"S5(Phospho): 99.59","ptmRS..Phospho.Site.Probabilities":"S(3): 0.0; S(4): 0.4; S(5): 99.6"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (W4)","Search.ID":"W","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ELDEIAASLENL","Annotated.Sequence":"[K].ELDEIAASLENL.[-]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.4222,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":658.83297,"Contaminant":"False","Aligned.m.z..Da.":658.83312,"MH...Da.":1316.65867,"Theo..MH...Da.":1316.658,"DeltaM..ppm.":0.51,"Deltam.z..Da.":0.00034,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":832129.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":20.37672,"Ion.Inject.Time..ms.":22,"RT..min.":18.4597,"First.Scan":19014,"Last.Scan":19014,"Master.Scan.s.":19007,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_02.raw","File.ID":"F14","Quan.Info":"","Peptides.Matched":673,"XCorr":1.8,"X..Protein.Groups":1,"Percolator.q.Value":0.00147,"Percolator.PEP":0.01575,"Percolator.SVMScore":0.509,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (G4)","Search.ID":"G","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"HSDSYSENETNHTNVPISSTGGTNNK","Annotated.Sequence":"[R].HSDSYSENETNHtNVPISSTGGTNNK.[T]","Modifications":"T13(Phospho)","X..Proteins":20,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527","Protein.Descriptions":"Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-GR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR1 PE=3 SV=1; Transposon Ty1-JR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR1 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-DR6 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR6 PE=3 SV=1; Transposon Ty1-GR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR2 PE=3 SV=3; Transposon Ty1-DR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR1 PE=3 SV=2; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-PL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PL PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OL PE=3 SV=1; Transposon Ty1-ER1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER1 PE=3 SV=1; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-LR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR2 PE=3 SV=1; Transposon Ty1-ML2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML2 PE=3 SV=2; Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-A PE=3 SV=3","X..Missed.Cleavages":0,"Charge":4,"Original.Precursor.Charge":4,"DeltaScore":0.0099,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":2,"m.z..Da.":718.30089,"Contaminant":"False","Aligned.m.z..Da.":718.30094,"MH...Da.":2870.18174,"Theo..MH...Da.":2870.18608,"DeltaM..ppm.":-1.51,"Deltam.z..Da.":-0.00109,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3123684.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":95.71088,"Ion.Inject.Time..ms.":4.555,"RT..min.":7.4873,"First.Scan":5604,"Last.Scan":5604,"Master.Scan.s.":5597,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_06.raw","File.ID":"F30","Quan.Info":"","Peptides.Matched":2337,"XCorr":1.01,"X..Protein.Groups":1,"Percolator.q.Value":0.00148,"Percolator.PEP":0.02095,"Percolator.SVMScore":0.466,"Precursor.Area":7219759.5,"Apex.RT..min.":7.5,"ptmRS..Binomial.Peptide.Score":161.15,"ptmRS..Isoform.Confidence.Probability":0.25,"ptmRS..Isoform.Group.Report":"b2+-H(3) O(4) P(14): 799.81; b2+-H(3) O(4) P(15): 849.34","ptmRS..Best.Site.Probabilities":"S4(Phospho): 24.99; S6(Phospho): 24.99; T10(Phospho): 24.99; T13(Phospho): 24.99","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(4): 25.0; Y(5): 0.0; S(6): 25.0; T(10): 25.0; T(13): 25.0; S(18): 0.0; S(19): 0.0; T(20): 0.0; T(23): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (L4)","Search.ID":"L","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KVIGSFK","Annotated.Sequence":"[K].KVIGSFK.[D]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.3043,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":389.74439,"Contaminant":"False","Aligned.m.z..Da.":389.7444,"MH...Da.":778.48151,"Theo..MH...Da.":778.48215,"DeltaM..ppm.":-0.82,"Deltam.z..Da.":-0.00032,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3492320.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":40.33561,"Ion.Inject.Time..ms.":22,"RT..min.":6.2248,"First.Scan":3117,"Last.Scan":3117,"Master.Scan.s.":3116,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_01.raw","File.ID":"F25","Quan.Info":"","Peptides.Matched":22,"XCorr":2.07,"X..Protein.Groups":1,"Percolator.q.Value":0.00152,"Percolator.PEP":0.01711,"Percolator.SVMScore":0.553,"Precursor.Area":3212570,"Apex.RT..min.":6.23,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (L4)","Search.ID":"L","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ELDEIAASLENL","Annotated.Sequence":"[K].ELDEIAASLENL.[-]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5873,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":658.83449,"Contaminant":"False","Aligned.m.z..Da.":658.83481,"MH...Da.":1316.66171,"Theo..MH...Da.":1316.658,"DeltaM..ppm.":2.81,"Deltam.z..Da.":0.00185,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":835469.6875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":5.54972,"Ion.Inject.Time..ms.":22,"RT..min.":18.4373,"First.Scan":19165,"Last.Scan":19165,"Master.Scan.s.":19161,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_01.raw","File.ID":"F25","Quan.Info":"","Peptides.Matched":637,"XCorr":2.52,"X..Protein.Groups":1,"Percolator.q.Value":0.00152,"Percolator.PEP":0.01803,"Percolator.SVMScore":0.542,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (G4)","Search.ID":"G","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KVIGSFK","Annotated.Sequence":"[K].KVIGSFK.[D]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1845,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":389.74437,"Contaminant":"False","Aligned.m.z..Da.":389.74433,"MH...Da.":778.48146,"Theo..MH...Da.":778.48215,"DeltaM..ppm.":-0.88,"Deltam.z..Da.":-0.00034,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2594378.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":66.88785,"Ion.Inject.Time..ms.":22,"RT..min.":6.2524,"First.Scan":3727,"Last.Scan":3727,"Master.Scan.s.":3725,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_06.raw","File.ID":"F30","Quan.Info":"","Peptides.Matched":22,"XCorr":1.68,"X..Protein.Groups":1,"Percolator.q.Value":0.00157,"Percolator.PEP":0.02443,"Percolator.SVMScore":0.433,"Precursor.Area":2848134.25,"Apex.RT..min.":6.39,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (U4)","Search.ID":"U","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KVIGSFK","Annotated.Sequence":"[K].KVIGSFK.[D]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.2637,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":389.74464,"Contaminant":"False","Aligned.m.z..Da.":389.74463,"MH...Da.":778.482,"Theo..MH...Da.":778.48215,"DeltaM..ppm.":-0.19,"Deltam.z..Da.":-0.00008,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1870663,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":52.09065,"Ion.Inject.Time..ms.":22,"RT..min.":6.2412,"First.Scan":3241,"Last.Scan":3241,"Master.Scan.s.":3229,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_04.raw","File.ID":"F16","Quan.Info":"","Peptides.Matched":22,"XCorr":2.01,"X..Protein.Groups":1,"Percolator.q.Value":0.0016,"Percolator.PEP":0.03984,"Percolator.SVMScore":0.319,"Precursor.Area":1543921.875,"Apex.RT..min.":6.24,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AG4)","Search.ID":"AG","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"SFLTYIK","Annotated.Sequence":"[K].SFLTYIK.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.4685,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":436.2485,"Contaminant":"False","Aligned.m.z..Da.":436.24859,"MH...Da.":871.48974,"Theo..MH...Da.":871.49238,"DeltaM..ppm.":-3.04,"Deltam.z..Da.":-0.00132,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":409635.78125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":13.6863,"First.Scan":13115,"Last.Scan":13115,"Master.Scan.s.":13106,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y25_04.raw","File.ID":"F4","Quan.Info":"","Peptides.Matched":310,"XCorr":1.11,"X..Protein.Groups":1,"Percolator.q.Value":0.00161,"Percolator.PEP":0.03267,"Percolator.SVMScore":0.333,"Precursor.Area":839625.625,"Apex.RT..min.":13.7,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Q4)","Search.ID":"Q","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ELDEIAASLENL","Annotated.Sequence":"[K].ELDEIAASLENL.[-]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.4833,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":658.8329,"Contaminant":"False","Aligned.m.z..Da.":658.83292,"MH...Da.":1316.65852,"Theo..MH...Da.":1316.658,"DeltaM..ppm.":0.39,"Deltam.z..Da.":0.00026,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1009727.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":16.93796,"Ion.Inject.Time..ms.":22,"RT..min.":18.4607,"First.Scan":19165,"Last.Scan":19165,"Master.Scan.s.":19161,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_02.raw","File.ID":"F20","Quan.Info":"","Peptides.Matched":675,"XCorr":1.8,"X..Protein.Groups":1,"Percolator.q.Value":0.00162,"Percolator.PEP":0.02461,"Percolator.SVMScore":0.465,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (S4)","Search.ID":"S","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"SFLTYIK","Annotated.Sequence":"[K].SFLTYIK.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5703,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":436.2486,"Contaminant":"False","Aligned.m.z..Da.":436.24865,"MH...Da.":871.48994,"Theo..MH...Da.":871.49238,"DeltaM..ppm.":-2.81,"Deltam.z..Da.":-0.00122,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1119672.375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":13.7619,"First.Scan":13821,"Last.Scan":13821,"Master.Scan.s.":13818,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_06.raw","File.ID":"F18","Quan.Info":"","Peptides.Matched":287,"XCorr":1.28,"X..Protein.Groups":1,"Percolator.q.Value":0.00165,"Percolator.PEP":0.02271,"Percolator.SVMScore":0.378,"Precursor.Area":3656669.5,"Apex.RT..min.":13.79,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (P4)","Search.ID":"P","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"VFDNIQR","Annotated.Sequence":"[R].VFDNIQR.[R]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.464,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":446.23817,"Contaminant":"False","Aligned.m.z..Da.":446.23825,"MH...Da.":891.46906,"Theo..MH...Da.":891.46829,"DeltaM..ppm.":0.86,"Deltam.z..Da.":0.00038,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":4672282.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":28.61539,"Ion.Inject.Time..ms.":17.696,"RT..min.":8.2581,"First.Scan":6247,"Last.Scan":6247,"Master.Scan.s.":6244,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_03.raw","File.ID":"F21","Quan.Info":"","Peptides.Matched":367,"XCorr":2.22,"X..Protein.Groups":1,"Percolator.q.Value":0.00166,"Percolator.PEP":0.0383,"Percolator.SVMScore":0.311,"Precursor.Area":4227335,"Apex.RT..min.":8.27,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AA4)","Search.ID":"AA","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"VFDNIQR","Annotated.Sequence":"[R].VFDNIQR.[R]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.3481,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":446.23835,"Contaminant":"False","Aligned.m.z..Da.":446.23839,"MH...Da.":891.46942,"Theo..MH...Da.":891.46829,"DeltaM..ppm.":1.27,"Deltam.z..Da.":0.00056,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1998496.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":66.27223,"Ion.Inject.Time..ms.":22,"RT..min.":8.2552,"First.Scan":6282,"Last.Scan":6282,"Master.Scan.s.":6276,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_04.raw","File.ID":"F10","Quan.Info":"","Peptides.Matched":363,"XCorr":1.58,"X..Protein.Groups":1,"Percolator.q.Value":0.00167,"Percolator.PEP":0.01194,"Percolator.SVMScore":0.481,"Precursor.Area":1348009.75,"Apex.RT..min.":8.26,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (X4)","Search.ID":"X","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"SFLTYIK","Annotated.Sequence":"[K].SFLTYIK.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6433,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":436.24969,"Contaminant":"False","Aligned.m.z..Da.":436.24978,"MH...Da.":871.4921,"Theo..MH...Da.":871.49238,"DeltaM..ppm.":-0.32,"Deltam.z..Da.":-0.00014,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":574326.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":2.97839,"Ion.Inject.Time..ms.":22,"RT..min.":13.6681,"First.Scan":12967,"Last.Scan":12967,"Master.Scan.s.":12961,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_01.raw","File.ID":"F13","Quan.Info":"","Peptides.Matched":273,"XCorr":1.57,"X..Protein.Groups":1,"Percolator.q.Value":0.00167,"Percolator.PEP":0.02532,"Percolator.SVMScore":0.431,"Precursor.Area":3851272,"Apex.RT..min.":13.7,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (I4)","Search.ID":"I","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"KSDEPSRESTPVR","Annotated.Sequence":"[K].KSDEPsREsTPVR.[S]","Modifications":"S6(Phospho); S9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q08421","Master.Protein.Descriptions":"Enhancer of translation termination 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ETT1 PE=1 SV=1","Protein.Accessions":"Q08421","Protein.Descriptions":"Enhancer of translation termination 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ETT1 PE=1 SV=1","X..Missed.Cleavages":2,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0031,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":549.89747,"Contaminant":"False","Aligned.m.z..Da.":549.89741,"MH...Da.":1647.67787,"Theo..MH...Da.":1647.67752,"DeltaM..ppm.":0.21,"Deltam.z..Da.":0.00012,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":9507780,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":24.30531,"Ion.Inject.Time..ms.":10.436,"RT..min.":4.5438,"First.Scan":1307,"Last.Scan":1307,"Master.Scan.s.":1296,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_04.raw","File.ID":"F28","Quan.Info":"","Peptides.Matched":968,"XCorr":3.23,"X..Protein.Groups":1,"Percolator.q.Value":0.00167,"Percolator.PEP":0.01925,"Percolator.SVMScore":0.419,"Precursor.Area":23172212,"Apex.RT..min.":4.55,"ptmRS..Binomial.Peptide.Score":197.13,"ptmRS..Isoform.Confidence.Probability":0.009,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(6): 750.32; y2+-H(3) O(4) P(12): 711.31; y2+-H(3) O(4) P(11): 667.79; y2+-H(3) O(4) P(10): 610.28; y2+- -2H(3) O(4) P -2H(3) O(4) P(12): 662.32; y2+- -2H(3) O(4) P -2H(3) O(4) P(11): 618.80; y2+- -2H(3) O(4) P -2H(3) O(4) P(10): 561.29","ptmRS..Best.Site.Probabilities":"S9(Phospho): 99.15; T10(Phospho): 99.15","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(6): 1.7; S(9): 99.2; T(10): 99.2"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AF4)","Search.ID":"AF","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ILISTDLLAR","Annotated.Sequence":"[R].ILISTDLLAR.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5782,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":557.84598,"Contaminant":"False","Aligned.m.z..Da.":557.84594,"MH...Da.":1114.68469,"Theo..MH...Da.":1114.68304,"DeltaM..ppm.":1.48,"Deltam.z..Da.":0.00083,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":121506.10156,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":76.14045,"Ion.Inject.Time..ms.":22,"RT..min.":15.624,"First.Scan":15414,"Last.Scan":15414,"Master.Scan.s.":15413,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y25_05.raw","File.ID":"F5","Quan.Info":"","Peptides.Matched":124,"XCorr":1.47,"X..Protein.Groups":1,"Percolator.q.Value":0.00169,"Percolator.PEP":0.04024,"Percolator.SVMScore":0.353,"Precursor.Area":338183.03125,"Apex.RT..min.":15.6,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Q4)","Search.ID":"Q","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"EDGTTPFVAIWK","Annotated.Sequence":"[R].EDGTTPFVAIWK.[H]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.4375,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":682.34604,"Contaminant":"False","Aligned.m.z..Da.":682.3458,"MH...Da.":1363.6848,"Theo..MH...Da.":1363.68924,"DeltaM..ppm.":-3.26,"Deltam.z..Da.":-0.00222,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":288153.8125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":28.28082,"Ion.Inject.Time..ms.":22,"RT..min.":16.9169,"First.Scan":17176,"Last.Scan":17176,"Master.Scan.s.":17165,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_02.raw","File.ID":"F20","Quan.Info":"","Peptides.Matched":650,"XCorr":1.12,"X..Protein.Groups":1,"Percolator.q.Value":0.00171,"Percolator.PEP":0.03112,"Percolator.SVMScore":0.417,"Precursor.Area":214063.32812,"Apex.RT..min.":16.91,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AA4)","Search.ID":"AA","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KVIGSFK","Annotated.Sequence":"[K].KVIGSFK.[D]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.3005,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":389.74484,"Contaminant":"False","Aligned.m.z..Da.":389.74483,"MH...Da.":778.4824,"Theo..MH...Da.":778.48215,"DeltaM..ppm.":0.32,"Deltam.z..Da.":0.00012,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1252851.125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":59.37945,"Ion.Inject.Time..ms.":22,"RT..min.":6.1989,"First.Scan":3193,"Last.Scan":3193,"Master.Scan.s.":3182,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_04.raw","File.ID":"F10","Quan.Info":"","Peptides.Matched":22,"XCorr":1.93,"X..Protein.Groups":1,"Percolator.q.Value":0.00175,"Percolator.PEP":0.01801,"Percolator.SVMScore":0.398,"Precursor.Area":898557,"Apex.RT..min.":6.2,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (H4)","Search.ID":"H","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"SSQIHIENESTEDILK","Annotated.Sequence":"[R].SSQIHIENEStEDILK.[I]","Modifications":"T11(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":2,"DeltaScore":0.0977,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":641.62908,"Contaminant":"False","Aligned.m.z..Da.":641.62898,"MH...Da.":1922.87269,"Theo..MH...Da.":1922.87429,"DeltaM..ppm.":-0.83,"Deltam.z..Da.":-0.00053,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1039871.6875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":60.6385,"Ion.Inject.Time..ms.":22,"RT..min.":11.2753,"First.Scan":10707,"Last.Scan":10707,"Master.Scan.s.":10697,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_05.raw","File.ID":"F29","Quan.Info":"","Peptides.Matched":1830,"XCorr":1.74,"X..Protein.Groups":1,"Percolator.q.Value":0.00175,"Percolator.PEP":0.02378,"Percolator.SVMScore":0.412,"Precursor.Area":841533.0625,"Apex.RT..min.":11.28,"ptmRS..Binomial.Peptide.Score":137.33,"ptmRS..Isoform.Confidence.Probability":0.987,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(7): 787.42; y1+-H(3) O(4) P(6): 700.39","ptmRS..Best.Site.Probabilities":"T11(Phospho): 98.69","ptmRS..Phospho.Site.Probabilities":"S(1): 0.0; S(2): 0.0; S(10): 1.3; T(11): 98.7"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (M4)","Search.ID":"M","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":2,"DeltaScore":0.0194,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40436,"Contaminant":"False","Aligned.m.z..Da.":967.40426,"MH...Da.":2900.19852,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":-0.28,"Deltam.z..Da.":-0.00027,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":8177132,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":61.51729,"Ion.Inject.Time..ms.":22,"RT..min.":15.0576,"First.Scan":15497,"Last.Scan":15497,"Master.Scan.s.":15487,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_06.raw","File.ID":"F24","Quan.Info":"","Peptides.Matched":2442,"XCorr":1.03,"X..Protein.Groups":1,"Percolator.q.Value":0.00176,"Percolator.PEP":0.03427,"Percolator.SVMScore":0.33,"Precursor.Area":25301892,"Apex.RT..min.":15.01,"ptmRS..Binomial.Peptide.Score":83.75,"ptmRS..Isoform.Confidence.Probability":0.333,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(2): 112.04; y2+(18): 1055.49","ptmRS..Best.Site.Probabilities":"S2(Phospho): 33.33; Y12(Phospho): 33.33; S13(Phospho): 33.33","ptmRS..Phospho.Site.Probabilities":"S(2): 33.3; Y(12): 33.3; S(13): 33.3; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (N4)","Search.ID":"N","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LTESTVNHTNHSDDELPGHLLLDSGASR","Annotated.Sequence":"[K].LtESTVNHTNHSDDELPGHLLLDSGASR.[T]","Modifications":"T2(Phospho)","X..Proteins":15,"Master.Protein.Accessions":"Q99231","Master.Protein.Descriptions":"Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2","Protein.Accessions":"Q99337; P0C2I9; P47100; P0C2J0; Q03619; P0C2I2; Q99231; Q12088; Q07793; Q04711; Q12316; O13535; Q04670; Q92393; Q12193","Protein.Descriptions":"Transposon Ty1-NL2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-NL2 PE=3 SV=1; Transposon Ty1-PR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR1 PE=3 SV=1; Transposon Ty1-JR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-JR2 PE=3 SV=3; Transposon Ty1-PR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-PR2 PE=3 SV=1; Transposon Ty1-ER2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ER2 PE=3 SV=1; Transposon Ty1-DR5 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR5 PE=3 SV=1; Transposon Ty1-DR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR3 PE=1 SV=2; Transposon Ty1-LR1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-LR1 PE=3 SV=1; Transposon Ty1-DR4 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-DR4 PE=3 SV=2; Transposon Ty1-ML1 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-ML1 PE=3 SV=2; Transposon Ty1-GR3 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-GR3 PE=3 SV=3; Transposon Ty1-H Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-H PE=3 SV=1; Transposon Ty1-MR2 Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-MR2 PE=3 SV=2; Transposon Ty1-OR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-OR PE=3 SV=1; Transposon Ty1-BR Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY1B-BR PE=3 SV=1","X..Missed.Cleavages":0,"Charge":4,"Original.Precursor.Charge":4,"DeltaScore":0.0785,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":774.60688,"Contaminant":"False","Aligned.m.z..Da.":774.60669,"MH...Da.":3095.40569,"Theo..MH...Da.":3095.40658,"DeltaM..ppm.":-0.29,"Deltam.z..Da.":-0.00022,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":46957.09766,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":63.75274,"Ion.Inject.Time..ms.":22,"RT..min.":12.2321,"First.Scan":12018,"Last.Scan":12018,"Master.Scan.s.":12007,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_05.raw","File.ID":"F23","Quan.Info":"","Peptides.Matched":4582,"XCorr":1.91,"X..Protein.Groups":1,"Percolator.q.Value":0.00177,"Percolator.PEP":0.04021,"Percolator.SVMScore":0.304,"Precursor.Area":281899.5,"Apex.RT..min.":12.2,"ptmRS..Binomial.Peptide.Score":137.6,"ptmRS..Isoform.Confidence.Probability":0.998,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(2): 197.13; b1+-H(3) O(4) P(3): 326.17","ptmRS..Best.Site.Probabilities":"T2(Phospho): 99.84","ptmRS..Phospho.Site.Probabilities":"T(2): 99.8; S(4): 0.0; T(5): 0.0; T(9): 0.0; S(12): 0.0; S(24): 0.0; S(27): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (L4)","Search.ID":"L","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"GVFGYGFEEPSAIQQR","Annotated.Sequence":"[R].GVFGYGFEEPSAIQQR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.4591,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":595.62574,"Contaminant":"False","Aligned.m.z..Da.":595.62577,"MH...Da.":1784.86269,"Theo..MH...Da.":1784.86022,"DeltaM..ppm.":1.38,"Deltam.z..Da.":0.00082,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":375271.625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":31.01284,"Ion.Inject.Time..ms.":22,"RT..min.":15.0222,"First.Scan":14713,"Last.Scan":14713,"Master.Scan.s.":14705,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_01.raw","File.ID":"F25","Quan.Info":"","Peptides.Matched":1390,"XCorr":1.59,"X..Protein.Groups":1,"Percolator.q.Value":0.00181,"Percolator.PEP":0.02447,"Percolator.SVMScore":0.479,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (L4)","Search.ID":"L","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"VFDNIQR","Annotated.Sequence":"[R].VFDNIQR.[R]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.4247,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":446.23821,"Contaminant":"False","Aligned.m.z..Da.":446.23828,"MH...Da.":891.46914,"Theo..MH...Da.":891.46829,"DeltaM..ppm.":0.95,"Deltam.z..Da.":0.00043,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":9419624,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":35.36737,"Ion.Inject.Time..ms.":10.988,"RT..min.":8.2626,"First.Scan":6246,"Last.Scan":6246,"Master.Scan.s.":6236,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_01.raw","File.ID":"F25","Quan.Info":"","Peptides.Matched":367,"XCorr":1.86,"X..Protein.Groups":1,"Percolator.q.Value":0.00181,"Percolator.PEP":0.02479,"Percolator.SVMScore":0.477,"Precursor.Area":5335867.5,"Apex.RT..min.":8.26,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AH4)","Search.ID":"AH","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"SFLTYIK","Annotated.Sequence":"[K].SFLTYIK.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5794,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":436.24802,"Contaminant":"False","Aligned.m.z..Da.":436.24803,"MH...Da.":871.48877,"Theo..MH...Da.":871.49238,"DeltaM..ppm.":-4.14,"Deltam.z..Da.":-0.0018,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":873620.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":13.6933,"First.Scan":13115,"Last.Scan":13115,"Master.Scan.s.":13109,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y25_03.raw","File.ID":"F3","Quan.Info":"","Peptides.Matched":305,"XCorr":1.26,"X..Protein.Groups":1,"Percolator.q.Value":0.00184,"Percolator.PEP":0.02462,"Percolator.SVMScore":0.35,"Precursor.Area":1245831.75,"Apex.RT..min.":13.71,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"IGNNELLHR","Annotated.Sequence":"[K].IGNNELLHR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q03558","Master.Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","Protein.Accessions":"Q03558","Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.0924,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":533.29309,"Contaminant":"False","Aligned.m.z..Da.":533.29309,"MH...Da.":1065.57891,"Theo..MH...Da.":1065.57997,"DeltaM..ppm.":-1,"Deltam.z..Da.":-0.00053,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":6000997,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":56.95644,"Ion.Inject.Time..ms.":14.342,"RT..min.":7.6175,"First.Scan":5381,"Last.Scan":5381,"Master.Scan.s.":5371,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":302,"XCorr":1.19,"X..Protein.Groups":1,"Percolator.q.Value":0.00188,"Percolator.PEP":0.02374,"Percolator.SVMScore":0.36,"Precursor.Area":6625931.5,"Apex.RT..min.":7.6,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (X4)","Search.ID":"X","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LQETNPEEVPKFEK","Annotated.Sequence":"[K].LQETNPEEVPKFEK.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.4286,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":563.2904,"Contaminant":"False","Aligned.m.z..Da.":563.29039,"MH...Da.":1687.85664,"Theo..MH...Da.":1687.85374,"DeltaM..ppm.":1.72,"Deltam.z..Da.":0.00097,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1405135.375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":74.1068,"Ion.Inject.Time..ms.":9.048,"RT..min.":9.3151,"First.Scan":7413,"Last.Scan":7413,"Master.Scan.s.":7405,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_01.raw","File.ID":"F13","Quan.Info":"","Peptides.Matched":870,"XCorr":0.91,"X..Protein.Groups":1,"Percolator.q.Value":0.0019,"Percolator.PEP":0.02681,"Percolator.SVMScore":0.419,"Precursor.Area":943132.3125,"Apex.RT..min.":9.31,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Q4)","Search.ID":"Q","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"VFDNIQR","Annotated.Sequence":"[R].VFDNIQR.[R]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.4378,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":446.23821,"Contaminant":"False","Aligned.m.z..Da.":446.23829,"MH...Da.":891.46915,"Theo..MH...Da.":891.46829,"DeltaM..ppm.":0.96,"Deltam.z..Da.":0.00043,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3626082.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":44.05844,"Ion.Inject.Time..ms.":18.122,"RT..min.":8.2539,"First.Scan":6250,"Last.Scan":6250,"Master.Scan.s.":6245,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_02.raw","File.ID":"F20","Quan.Info":"","Peptides.Matched":367,"XCorr":2.01,"X..Protein.Groups":1,"Percolator.q.Value":0.00191,"Percolator.PEP":0.0346,"Percolator.SVMScore":0.396,"Precursor.Area":3863509.75,"Apex.RT..min.":8.26,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].mSEGItDIEEsQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss); T6(Phospho); S11(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.1017,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":743.96245,"Contaminant":"False","Aligned.m.z..Da.":743.96247,"MH...Da.":2229.8728,"Theo..MH...Da.":2229.88361,"DeltaM..ppm.":-4.85,"Deltam.z..Da.":-0.0036,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":812850.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":33.04409,"Ion.Inject.Time..ms.":22,"RT..min.":15.7113,"First.Scan":15850,"Last.Scan":15850,"Master.Scan.s.":15841,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":969,"XCorr":1.77,"X..Protein.Groups":1,"Percolator.q.Value":0.00194,"Percolator.PEP":0.02749,"Percolator.SVMScore":0.349,"Precursor.Area":665446.8125,"Apex.RT..min.":15.71,"ptmRS..Binomial.Peptide.Score":114.07,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"b2+-H(3) O(4) P(9): 454.18","ptmRS..Best.Site.Probabilities":"S2(Phospho): 99.96; T6(Phospho): 98.05","ptmRS..Phospho.Site.Probabilities":"S(2): 100.0; T(6): 98.1; S(11): 2.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"VFDNIQR","Annotated.Sequence":"[R].VFDNIQR.[R]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.4194,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":446.23778,"Contaminant":"False","Aligned.m.z..Da.":446.23781,"MH...Da.":891.46828,"Theo..MH...Da.":891.46829,"DeltaM..ppm.":-0.01,"Deltam.z..Da.":-1e-05,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":8517533,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":30.74339,"Ion.Inject.Time..ms.":10.455,"RT..min.":8.2695,"First.Scan":6172,"Last.Scan":6172,"Master.Scan.s.":6169,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":426,"XCorr":2.17,"X..Protein.Groups":1,"Percolator.q.Value":0.00194,"Percolator.PEP":0.02662,"Percolator.SVMScore":0.355,"Precursor.Area":19375940,"Apex.RT..min.":8.28,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"VEELTTK","Annotated.Sequence":"[K].VEELTTK.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.592,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":410.22645,"Contaminant":"False","Aligned.m.z..Da.":410.22645,"MH...Da.":819.44562,"Theo..MH...Da.":819.44582,"DeltaM..ppm.":-0.25,"Deltam.z..Da.":-0.0001,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":10586879,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":16.71803,"Ion.Inject.Time..ms.":11.998,"RT..min.":5.5581,"First.Scan":2672,"Last.Scan":2672,"Master.Scan.s.":2667,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":120,"XCorr":2.01,"X..Protein.Groups":1,"Percolator.q.Value":0.00196,"Percolator.PEP":0.0245,"Percolator.SVMScore":0.354,"Precursor.Area":10528264,"Apex.RT..min.":5.57,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"KSDEPSRESTPVR","Annotated.Sequence":"[K].KSDEPSREstPVR.[S]","Modifications":"S9(Phospho); T10(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q08421","Master.Protein.Descriptions":"Enhancer of translation termination 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ETT1 PE=1 SV=1","Protein.Accessions":"Q08421","Protein.Descriptions":"Enhancer of translation termination 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ETT1 PE=1 SV=1","X..Missed.Cleavages":2,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0091,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":549.89664,"Contaminant":"False","Aligned.m.z..Da.":549.89664,"MH...Da.":1647.67535,"Theo..MH...Da.":1647.67752,"DeltaM..ppm.":-1.32,"Deltam.z..Da.":-0.00072,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":13522848,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":8.30973,"Ion.Inject.Time..ms.":10.165,"RT..min.":4.5496,"First.Scan":1346,"Last.Scan":1346,"Master.Scan.s.":1341,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":912,"XCorr":3.3,"X..Protein.Groups":1,"Percolator.q.Value":0.00202,"Percolator.PEP":0.02678,"Percolator.SVMScore":0.337,"Precursor.Area":52995544,"Apex.RT..min.":4.56,"ptmRS..Binomial.Peptide.Score":229.43,"ptmRS..Isoform.Confidence.Probability":0.004,"ptmRS..Isoform.Group.Report":"y2+-H(3) O(4) P(12): 711.31; y2+-H(3) O(4) P(11): 667.79; y2+-H(3) O(4) P(10): 610.28; y2+-H(3) O(4) P(7): 413.73; b1+- -2H(3) O(4) P -2H(3) O(4) P(10): 1081.49; b2+- -2H(3) O(4) P -2H(3) O(4) P(10): 541.25","ptmRS..Best.Site.Probabilities":"S6(Phospho): 99.57; S9(Phospho): 50.21; T10(Phospho): 50.21","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(6): 99.6; S(9): 50.2; T(10): 50.2"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AA4)","Search.ID":"AA","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"SFLTYIK","Annotated.Sequence":"[K].SFLTYIK.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5891,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":436.24851,"Contaminant":"False","Aligned.m.z..Da.":436.2486,"MH...Da.":871.48975,"Theo..MH...Da.":871.49238,"DeltaM..ppm.":-3.01,"Deltam.z..Da.":-0.00131,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":843240.4375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":13.6902,"First.Scan":13172,"Last.Scan":13172,"Master.Scan.s.":13166,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_04.raw","File.ID":"F10","Quan.Info":"","Peptides.Matched":310,"XCorr":1.29,"X..Protein.Groups":1,"Percolator.q.Value":0.00205,"Percolator.PEP":0.02307,"Percolator.SVMScore":0.35,"Precursor.Area":1911645,"Apex.RT..min.":13.71,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (M4)","Search.ID":"M","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KFQDDTLNR","Annotated.Sequence":"[K].KFQDDtLNR.[V]","Modifications":"T6(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":4,"DeltaScore":0.5179,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":608.76897,"Contaminant":"False","Aligned.m.z..Da.":608.76895,"MH...Da.":1216.53067,"Theo..MH...Da.":1216.53579,"DeltaM..ppm.":-4.21,"Deltam.z..Da.":-0.00256,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1483168.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":61.75563,"Ion.Inject.Time..ms.":22,"RT..min.":6.2607,"First.Scan":3683,"Last.Scan":3683,"Master.Scan.s.":3672,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_06.raw","File.ID":"F24","Quan.Info":"","Peptides.Matched":595,"XCorr":1.68,"X..Protein.Groups":1,"Percolator.q.Value":0.00206,"Percolator.PEP":0.04571,"Percolator.SVMScore":0.273,"Precursor.Area":2167041.5,"Apex.RT..min.":6.23,"ptmRS..Binomial.Peptide.Score":174.52,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"T6(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"T(6): 100.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (O4)","Search.ID":"O","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"VFDNIQR","Annotated.Sequence":"[R].VFDNIQR.[R]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.4033,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":446.23837,"Contaminant":"False","Aligned.m.z..Da.":446.23843,"MH...Da.":891.46946,"Theo..MH...Da.":891.46829,"DeltaM..ppm.":1.32,"Deltam.z..Da.":0.00059,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":6686031.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":36.96391,"Ion.Inject.Time..ms.":14.06,"RT..min.":8.2651,"First.Scan":6242,"Last.Scan":6242,"Master.Scan.s.":6231,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_04.raw","File.ID":"F22","Quan.Info":"","Peptides.Matched":363,"XCorr":1.81,"X..Protein.Groups":1,"Percolator.q.Value":0.00208,"Percolator.PEP":0.03622,"Percolator.SVMScore":0.325,"Precursor.Area":4143790,"Apex.RT..min.":8.26,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AE4)","Search.ID":"AE","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"SFLTYIK","Annotated.Sequence":"[K].SFLTYIK.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5676,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":436.24962,"Contaminant":"False","Aligned.m.z..Da.":436.2496,"MH...Da.":871.49196,"Theo..MH...Da.":871.49238,"DeltaM..ppm.":-0.48,"Deltam.z..Da.":-0.00021,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":856545,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":21.32272,"Ion.Inject.Time..ms.":22,"RT..min.":13.8041,"First.Scan":13915,"Last.Scan":13915,"Master.Scan.s.":13913,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y25_06.raw","File.ID":"F6","Quan.Info":"","Peptides.Matched":273,"XCorr":1.48,"X..Protein.Groups":1,"Percolator.q.Value":0.00208,"Percolator.PEP":0.02189,"Percolator.SVMScore":0.401,"Precursor.Area":448395.65625,"Apex.RT..min.":13.81,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (W4)","Search.ID":"W","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ELDEIAASLENL","Annotated.Sequence":"[K].ELDEIAAsLENL.[-]","Modifications":"S8(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7033,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":698.81611,"Contaminant":"False","Aligned.m.z..Da.":698.81622,"MH...Da.":1396.62495,"Theo..MH...Da.":1396.62433,"DeltaM..ppm.":0.44,"Deltam.z..Da.":0.00031,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1088232,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":18.2387,"First.Scan":18726,"Last.Scan":18726,"Master.Scan.s.":18721,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_02.raw","File.ID":"F14","Quan.Info":"","Peptides.Matched":852,"XCorr":2.09,"X..Protein.Groups":1,"Percolator.q.Value":0.0021,"Percolator.PEP":0.02702,"Percolator.SVMScore":0.398,"Precursor.Area":1050937.625,"Apex.RT..min.":18.25,"ptmRS..Binomial.Peptide.Score":171.78,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"S8(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(8): 100.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"VELGLSHFPESQFLK","Annotated.Sequence":"[R].VELGLSHFPESQFLK.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q08421","Master.Protein.Descriptions":"Enhancer of translation termination 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ETT1 PE=1 SV=1","Protein.Accessions":"Q08421","Protein.Descriptions":"Enhancer of translation termination 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ETT1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":2,"DeltaScore":0.358,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":577.64166,"Contaminant":"False","Aligned.m.z..Da.":577.64166,"MH...Da.":1730.91041,"Theo..MH...Da.":1730.9112,"DeltaM..ppm.":-0.45,"Deltam.z..Da.":-0.00026,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":433611.40625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":39.09069,"Ion.Inject.Time..ms.":22,"RT..min.":15.7715,"First.Scan":15980,"Last.Scan":15980,"Master.Scan.s.":15979,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":628,"XCorr":1.76,"X..Protein.Groups":1,"Percolator.q.Value":0.0021,"Percolator.PEP":0.03293,"Percolator.SVMScore":0.297,"Precursor.Area":658997.875,"Apex.RT..min.":15.76,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"VFDNIQR","Annotated.Sequence":"[R].VFDNIQR.[R]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.4273,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":446.23824,"Contaminant":"False","Aligned.m.z..Da.":446.23824,"MH...Da.":891.4692,"Theo..MH...Da.":891.46829,"DeltaM..ppm.":1.02,"Deltam.z..Da.":0.00045,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":17752274,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":33.12459,"Ion.Inject.Time..ms.":4.94,"RT..min.":8.2756,"First.Scan":6245,"Last.Scan":6245,"Master.Scan.s.":6242,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":367,"XCorr":2.2,"X..Protein.Groups":1,"Percolator.q.Value":0.0021,"Percolator.PEP":0.03148,"Percolator.SVMScore":0.306,"Precursor.Area":49021200,"Apex.RT..min.":8.29,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Q4)","Search.ID":"Q","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KVIGSFK","Annotated.Sequence":"[K].KVIGSFK.[D]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.2412,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":389.74468,"Contaminant":"False","Aligned.m.z..Da.":389.74468,"MH...Da.":778.48208,"Theo..MH...Da.":778.48215,"DeltaM..ppm.":-0.08,"Deltam.z..Da.":-0.00003,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2586609.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":50.42871,"Ion.Inject.Time..ms.":22,"RT..min.":6.2112,"First.Scan":3140,"Last.Scan":3140,"Master.Scan.s.":3138,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_02.raw","File.ID":"F20","Quan.Info":"","Peptides.Matched":22,"XCorr":1.99,"X..Protein.Groups":1,"Percolator.q.Value":0.00211,"Percolator.PEP":0.04094,"Percolator.SVMScore":0.361,"Precursor.Area":1843666.75,"Apex.RT..min.":6.21,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (K4)","Search.ID":"K","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DFLLKPELSR","Annotated.Sequence":"[K].DFLLKPELSR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5568,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":609.34859,"Contaminant":"False","Aligned.m.z..Da.":609.34869,"MH...Da.":1217.68991,"Theo..MH...Da.":1217.68885,"DeltaM..ppm.":0.88,"Deltam.z..Da.":0.00053,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":323308.125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":26.0237,"Ion.Inject.Time..ms.":22,"RT..min.":13.1657,"First.Scan":12525,"Last.Scan":12525,"Master.Scan.s.":12523,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_02.raw","File.ID":"F26","Quan.Info":"","Peptides.Matched":265,"XCorr":1.76,"X..Protein.Groups":1,"Percolator.q.Value":0.00214,"Percolator.PEP":0.02938,"Percolator.SVMScore":0.392,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AA4)","Search.ID":"AA","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ILISTDLLAR","Annotated.Sequence":"[R].ILISTDLLAR.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5075,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":557.84508,"Contaminant":"False","Aligned.m.z..Da.":557.84519,"MH...Da.":1114.68289,"Theo..MH...Da.":1114.68304,"DeltaM..ppm.":-0.13,"Deltam.z..Da.":-0.00008,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":327893.875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":37.87246,"Ion.Inject.Time..ms.":22,"RT..min.":15.6371,"First.Scan":15452,"Last.Scan":15452,"Master.Scan.s.":15445,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_04.raw","File.ID":"F10","Quan.Info":"","Peptides.Matched":124,"XCorr":1.34,"X..Protein.Groups":1,"Percolator.q.Value":0.00215,"Percolator.PEP":0.02906,"Percolator.SVMScore":0.308,"Precursor.Area":582590.1875,"Apex.RT..min.":15.6,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (H4)","Search.ID":"H","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSSPLHRLSPTGK","Annotated.Sequence":"[R].mSsPLHRLsPTGK.[S]","Modifications":"M1(Oxidation); S3(Phospho); S9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.4388,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":793.8439,"Contaminant":"False","Aligned.m.z..Da.":793.84406,"MH...Da.":1586.68052,"Theo..MH...Da.":1586.67977,"DeltaM..ppm.":0.47,"Deltam.z..Da.":0.00037,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3284560.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":56.99408,"Ion.Inject.Time..ms.":22,"RT..min.":7.725,"First.Scan":5972,"Last.Scan":5972,"Master.Scan.s.":5965,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_05.raw","File.ID":"F29","Quan.Info":"","Peptides.Matched":1055,"XCorr":1.39,"X..Protein.Groups":1,"Percolator.q.Value":0.00216,"Percolator.PEP":0.03176,"Percolator.SVMScore":0.348,"Precursor.Area":3408272,"Apex.RT..min.":7.73,"ptmRS..Binomial.Peptide.Score":134.65,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(3): 304.10; y2+-H(3) O(4) P(11): 627.82; y2+- -2H(3) O(4) P -2H(3) O(4) P(11): 578.83","ptmRS..Best.Site.Probabilities":"S3(Phospho): 99.98; S9(Phospho): 99.98","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(3): 100.0; S(9): 100.0; T(11): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AB4)","Search.ID":"AB","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"GVFGYGFEEPSAIQQR","Annotated.Sequence":"[R].GVFGYGFEEPSAIQQR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1812,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":892.93501,"Contaminant":"False","Aligned.m.z..Da.":892.9351,"MH...Da.":1784.86274,"Theo..MH...Da.":1784.86022,"DeltaM..ppm.":1.41,"Deltam.z..Da.":0.00126,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":263039.375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":66.70072,"Ion.Inject.Time..ms.":22,"RT..min.":15.0571,"First.Scan":14738,"Last.Scan":14738,"Master.Scan.s.":14737,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_03.raw","File.ID":"F9","Quan.Info":"","Peptides.Matched":1391,"XCorr":1.49,"X..Protein.Groups":1,"Percolator.q.Value":0.00234,"Percolator.PEP":0.03729,"Percolator.SVMScore":0.268,"Precursor.Area":332715.5,"Apex.RT..min.":15.04,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (H4)","Search.ID":"H","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].msHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss); S2(Phospho); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":4,"Original.Precursor.Charge":4,"DeltaScore":0.3476,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":735.29195,"Contaminant":"False","Aligned.m.z..Da.":735.29192,"MH...Da.":2938.146,"Theo..MH...Da.":2938.15509,"DeltaM..ppm.":-3.09,"Deltam.z..Da.":-0.00227,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":444480.8125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":21.28021,"Ion.Inject.Time..ms.":22,"RT..min.":14.9545,"First.Scan":15450,"Last.Scan":15450,"Master.Scan.s.":15447,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_05.raw","File.ID":"F29","Quan.Info":"","Peptides.Matched":1148,"XCorr":1.87,"X..Protein.Groups":1,"Percolator.q.Value":0.00236,"Percolator.PEP":0.0341,"Percolator.SVMScore":0.332,"Precursor.Area":535441.8125,"Apex.RT..min.":14.97,"ptmRS..Binomial.Peptide.Score":98.55,"ptmRS..Isoform.Confidence.Probability":0.333,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"S2(Phospho): 66.67; Y12(Phospho): 66.67; S13(Phospho): 66.67","ptmRS..Phospho.Site.Probabilities":"S(2): 66.7; Y(12): 66.7; S(13): 66.7; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (O4)","Search.ID":"O","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"IQDPQMTGYVSTR","Annotated.Sequence":"[R].IQDPQMtGYVsTR.[Y]","Modifications":"T7(Phospho); S11(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P32485","Master.Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","Protein.Accessions":"P32485","Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.0429,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":828.32926,"Contaminant":"False","Aligned.m.z..Da.":828.32931,"MH...Da.":1655.65125,"Theo..MH...Da.":1655.65362,"DeltaM..ppm.":-1.43,"Deltam.z..Da.":-0.00118,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1715229.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":67.55022,"Ion.Inject.Time..ms.":22,"RT..min.":9.8996,"First.Scan":8218,"Last.Scan":8218,"Master.Scan.s.":8207,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_04.raw","File.ID":"F22","Quan.Info":"","Peptides.Matched":727,"XCorr":1.63,"X..Protein.Groups":1,"Percolator.q.Value":0.00237,"Percolator.PEP":0.0417,"Percolator.SVMScore":0.297,"Precursor.Area":1226269,"Apex.RT..min.":9.9,"ptmRS..Binomial.Peptide.Score":229.83,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(11): 1316.53; y1+-H(3) O(4) P(10): 1201.51; y1+-H(3) O(4) P(8): 976.40; y1+-H(3) O(4) P(7): 845.36; y2+-H(3) O(4) P(10): 601.26; y1+(4): 462.27; y1+(3): 363.20","ptmRS..Best.Site.Probabilities":"T7(Phospho): 100; Y9(Phospho): 99.4","ptmRS..Phospho.Site.Probabilities":"T(7): 100.0; Y(9): 99.4; S(11): 0.0; T(12): 0.6"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (I4)","Search.ID":"I","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KVIGSFK","Annotated.Sequence":"[K].KVIGSFK.[D]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1852,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":389.74473,"Contaminant":"False","Aligned.m.z..Da.":389.74472,"MH...Da.":778.4822,"Theo..MH...Da.":778.48215,"DeltaM..ppm.":0.06,"Deltam.z..Da.":0.00002,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1654126,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":82.39518,"Ion.Inject.Time..ms.":22,"RT..min.":6.3563,"First.Scan":3434,"Last.Scan":3434,"Master.Scan.s.":3428,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_04.raw","File.ID":"F28","Quan.Info":"","Peptides.Matched":22,"XCorr":1.62,"X..Protein.Groups":1,"Percolator.q.Value":0.00242,"Percolator.PEP":0.03272,"Percolator.SVMScore":0.316,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"TALNIPK","Annotated.Sequence":"[K].TALNIPK.[K]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P39517","Master.Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","Protein.Accessions":"P39517","Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.2783,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":378.73473,"Contaminant":"False","Aligned.m.z..Da.":378.73475,"MH...Da.":756.46218,"Theo..MH...Da.":756.46142,"DeltaM..ppm.":1.01,"Deltam.z..Da.":0.00038,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1818655.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":81.46461,"Ion.Inject.Time..ms.":16.124,"RT..min.":9.0451,"First.Scan":7282,"Last.Scan":7282,"Master.Scan.s.":7274,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":94,"XCorr":1.15,"X..Protein.Groups":1,"Percolator.q.Value":0.0025,"Percolator.PEP":0.04049,"Percolator.SVMScore":0.277,"Precursor.Area":1257567.75,"Apex.RT..min.":9.04,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"VEELTTK","Annotated.Sequence":"[K].VEELTTK.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6146,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":410.22684,"Contaminant":"False","Aligned.m.z..Da.":410.22686,"MH...Da.":819.4464,"Theo..MH...Da.":819.44582,"DeltaM..ppm.":0.7,"Deltam.z..Da.":0.00029,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3527802.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":11.5952,"Ion.Inject.Time..ms.":22,"RT..min.":5.7143,"First.Scan":3105,"Last.Scan":3105,"Master.Scan.s.":3101,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":125,"XCorr":2.05,"X..Protein.Groups":1,"Percolator.q.Value":0.0025,"Percolator.PEP":0.04067,"Percolator.SVMScore":0.276,"Precursor.Area":6024426.5,"Apex.RT..min.":5.74,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (I4)","Search.ID":"I","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSSPLHRLSPTGK","Annotated.Sequence":"[R].mSsPLHRLsPTGK.[S]","Modifications":"M1(Oxidation); S3(Phospho); S9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5072,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":793.8423,"Contaminant":"False","Aligned.m.z..Da.":793.84236,"MH...Da.":1586.67732,"Theo..MH...Da.":1586.67977,"DeltaM..ppm.":-1.54,"Deltam.z..Da.":-0.00122,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2787812.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":67.83303,"Ion.Inject.Time..ms.":22,"RT..min.":7.7306,"First.Scan":5606,"Last.Scan":5606,"Master.Scan.s.":5599,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_04.raw","File.ID":"F28","Quan.Info":"","Peptides.Matched":960,"XCorr":1.38,"X..Protein.Groups":1,"Percolator.q.Value":0.0025,"Percolator.PEP":0.03457,"Percolator.SVMScore":0.305,"Precursor.Area":2750690.25,"Apex.RT..min.":7.74,"ptmRS..Binomial.Peptide.Score":131.74,"ptmRS..Isoform.Confidence.Probability":0.97,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(3): 304.10; b2+-H(3) O(4) P(6): 326.15; y2+- -2H(3) O(4) P -2H(3) O(4) P(11): 578.83","ptmRS..Best.Site.Probabilities":"S3(Phospho): 98.48; S9(Phospho): 98.48","ptmRS..Phospho.Site.Probabilities":"S(2): 1.5; S(3): 98.5; S(9): 98.5; T(11): 1.5"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (I4)","Search.ID":"I","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ELAYQIR","Annotated.Sequence":"[R].ELAYQIR.[N]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.2222,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":446.74759,"Contaminant":"False","Aligned.m.z..Da.":446.74758,"MH...Da.":892.4879,"Theo..MH...Da.":892.48869,"DeltaM..ppm.":-0.89,"Deltam.z..Da.":-0.0004,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1423009.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":87.83151,"Ion.Inject.Time..ms.":22,"RT..min.":9.5566,"First.Scan":7782,"Last.Scan":7782,"Master.Scan.s.":7770,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_04.raw","File.ID":"F28","Quan.Info":"","Peptides.Matched":394,"XCorr":0.99,"X..Protein.Groups":1,"Percolator.q.Value":0.0025,"Percolator.PEP":0.04019,"Percolator.SVMScore":0.277,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (J4)","Search.ID":"J","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KVIGSFK","Annotated.Sequence":"[K].KVIGSFK.[D]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1512,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":389.74469,"Contaminant":"False","Aligned.m.z..Da.":389.74465,"MH...Da.":778.48211,"Theo..MH...Da.":778.48215,"DeltaM..ppm.":-0.05,"Deltam.z..Da.":-0.00002,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2546012,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":78.71977,"Ion.Inject.Time..ms.":18.549,"RT..min.":6.3616,"First.Scan":3390,"Last.Scan":3390,"Master.Scan.s.":3386,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_03.raw","File.ID":"F27","Quan.Info":"","Peptides.Matched":22,"XCorr":1.72,"X..Protein.Groups":1,"Percolator.q.Value":0.00251,"Percolator.PEP":0.03181,"Percolator.SVMScore":0.316,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (M4)","Search.ID":"M","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"SFLTYIK","Annotated.Sequence":"[K].SFLTYIK.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6645,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":436.25049,"Contaminant":"False","Aligned.m.z..Da.":436.25058,"MH...Da.":871.49371,"Theo..MH...Da.":871.49238,"DeltaM..ppm.":1.52,"Deltam.z..Da.":0.00066,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":330898.1875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":6.83877,"Ion.Inject.Time..ms.":22,"RT..min.":13.7434,"First.Scan":13926,"Last.Scan":13926,"Master.Scan.s.":13916,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_06.raw","File.ID":"F24","Quan.Info":"","Peptides.Matched":57,"XCorr":1.55,"X..Protein.Groups":1,"Percolator.q.Value":0.00258,"Percolator.PEP":0.05188,"Percolator.SVMScore":0.248,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"AQERMSSPLHRLSPTGK","Annotated.Sequence":"[R].AQERmssPLHRLsPTGK.[S]","Modifications":"M5(Oxidation); S6(Phospho); S7(Phospho); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":2,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.2796,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":717.63335,"Contaminant":"False","Aligned.m.z..Da.":717.6334,"MH...Da.":2150.88551,"Theo..MH...Da.":2150.8855,"DeltaM..ppm.":0,"Deltam.z..Da.":0,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2512133,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":76.80367,"Ion.Inject.Time..ms.":17.728,"RT..min.":8.0661,"First.Scan":5871,"Last.Scan":5871,"Master.Scan.s.":5870,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":1369,"XCorr":1.86,"X..Protein.Groups":1,"Percolator.q.Value":0.00259,"Percolator.PEP":0.04153,"Percolator.SVMScore":0.272,"Precursor.Area":1857093.875,"Apex.RT..min.":8.06,"ptmRS..Binomial.Peptide.Score":89.24,"ptmRS..Isoform.Confidence.Probability":0.999,"ptmRS..Isoform.Group.Report":"y2+-H(3) O(4) P(10): 544.32; b2+- -2H(3) O(4) P -2H(3) O(4) P(13): 777.36; y1+(4): 402.23","ptmRS..Best.Site.Probabilities":"S6(Phospho): 100; S7(Phospho): 100; S13(Phospho): 99.92","ptmRS..Phospho.Site.Probabilities":"S(6): 100.0; S(7): 100.0; S(13): 99.9; T(15): 0.1"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"VFDNIQR","Annotated.Sequence":"[R].VFDNIQR.[R]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.3657,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":446.23784,"Contaminant":"False","Aligned.m.z..Da.":446.23787,"MH...Da.":891.4684,"Theo..MH...Da.":891.46829,"DeltaM..ppm.":0.12,"Deltam.z..Da.":0.00005,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1284881.375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":92.17946,"Ion.Inject.Time..ms.":15.432,"RT..min.":8.2644,"First.Scan":6166,"Last.Scan":6166,"Master.Scan.s.":6156,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":426,"XCorr":1.75,"X..Protein.Groups":1,"Percolator.q.Value":0.00259,"Percolator.PEP":0.04338,"Percolator.SVMScore":0.264,"Precursor.Area":19375940,"Apex.RT..min.":8.28,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AE4)","Search.ID":"AE","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MGSINNNFNTNNNSNTDLDRDWK","Annotated.Sequence":"[-].mGSINNNFNtNNNSNTDLDRDWK.[T]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); T10(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P39517","Master.Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","Protein.Accessions":"P39517","Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.2387,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":892.37373,"Contaminant":"False","Aligned.m.z..Da.":892.37368,"MH...Da.":2675.10666,"Theo..MH...Da.":2675.11179,"DeltaM..ppm.":-1.92,"Deltam.z..Da.":-0.00171,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":185028.90625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":6.5735,"Ion.Inject.Time..ms.":22,"RT..min.":13.4734,"First.Scan":13546,"Last.Scan":13546,"Master.Scan.s.":13542,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y25_06.raw","File.ID":"F6","Quan.Info":"","Peptides.Matched":2156,"XCorr":1.55,"X..Protein.Groups":1,"Percolator.q.Value":0.00263,"Percolator.PEP":0.04061,"Percolator.SVMScore":0.281,"Precursor.Area":206242.0625,"Apex.RT..min.":13.48,"ptmRS..Binomial.Peptide.Score":132.76,"ptmRS..Isoform.Confidence.Probability":0.496,"ptmRS..Isoform.Group.Report":"y2+-H(3) O(4) P(16): 968.43; y2+-H(3) O(4) P(15): 894.90","ptmRS..Best.Site.Probabilities":"T10(Phospho): 49.62; S14(Phospho): 49.62","ptmRS..Phospho.Site.Probabilities":"S(3): 0.0; T(10): 49.6; S(14): 49.6; T(16): 0.8"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (G4)","Search.ID":"G","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].msHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Acetyl); S2(Phospho); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":4,"Original.Precursor.Charge":4,"DeltaScore":0.2682,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":778.55654,"Contaminant":"False","Aligned.m.z..Da.":778.55656,"MH...Da.":3111.20433,"Theo..MH...Da.":3111.20614,"DeltaM..ppm.":-0.58,"Deltam.z..Da.":-0.00045,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1282255,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":9.5403,"First.Scan":8298,"Last.Scan":8298,"Master.Scan.s.":8288,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_06.raw","File.ID":"F30","Quan.Info":"","Peptides.Matched":750,"XCorr":2.2,"X..Protein.Groups":1,"Percolator.q.Value":0.00266,"Percolator.PEP":0.03093,"Percolator.SVMScore":0.384,"ptmRS..Binomial.Peptide.Score":158.73,"ptmRS..Isoform.Confidence.Probability":0.019,"ptmRS..Isoform.Group.Report":"b1+(11): 1312.54","ptmRS..Best.Site.Probabilities":"Y12(Phospho): 98.13; S13(Phospho): 99.96","ptmRS..Phospho.Site.Probabilities":"S(2): 1.9; Y(12): 98.1; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (L4)","Search.ID":"L","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ELDEIAASLENL","Annotated.Sequence":"[K].ELDEIAAsLENL.[-]","Modifications":"S8(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.7229,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":698.81666,"Contaminant":"False","Aligned.m.z..Da.":698.817,"MH...Da.":1396.62604,"Theo..MH...Da.":1396.62433,"DeltaM..ppm.":1.22,"Deltam.z..Da.":0.00085,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":525345.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":18.2196,"First.Scan":18869,"Last.Scan":18869,"Master.Scan.s.":18862,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_01.raw","File.ID":"F25","Quan.Info":"","Peptides.Matched":809,"XCorr":2.49,"X..Protein.Groups":1,"Percolator.q.Value":0.00268,"Percolator.PEP":0.03766,"Percolator.SVMScore":0.389,"Precursor.Area":1411525,"Apex.RT..min.":18.23,"ptmRS..Binomial.Peptide.Score":205.77,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"S8(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(8): 100.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Y4)","Search.ID":"Y","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KVIGSFK","Annotated.Sequence":"[K].KVIGSFK.[D]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.2073,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":389.74436,"Contaminant":"False","Aligned.m.z..Da.":389.74432,"MH...Da.":778.48144,"Theo..MH...Da.":778.48215,"DeltaM..ppm.":-0.91,"Deltam.z..Da.":-0.00035,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1174129.625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":59.94278,"Ion.Inject.Time..ms.":22,"RT..min.":6.2215,"First.Scan":3726,"Last.Scan":3726,"Master.Scan.s.":3717,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_06.raw","File.ID":"F12","Quan.Info":"","Peptides.Matched":22,"XCorr":1.64,"X..Protein.Groups":1,"Percolator.q.Value":0.0027,"Percolator.PEP":0.0351,"Percolator.SVMScore":0.331,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (W4)","Search.ID":"W","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"GVFGYGFEEPSAIQQR","Annotated.Sequence":"[R].GVFGYGFEEPSAIQQR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.3406,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":892.9326,"Contaminant":"False","Aligned.m.z..Da.":892.93263,"MH...Da.":1784.85793,"Theo..MH...Da.":1784.86022,"DeltaM..ppm.":-1.29,"Deltam.z..Da.":-0.00115,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":517585.40625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":66.64899,"Ion.Inject.Time..ms.":22,"RT..min.":15.0395,"First.Scan":14781,"Last.Scan":14781,"Master.Scan.s.":14780,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_02.raw","File.ID":"F14","Quan.Info":"","Peptides.Matched":1375,"XCorr":1.38,"X..Protein.Groups":1,"Percolator.q.Value":0.0028,"Percolator.PEP":0.03989,"Percolator.SVMScore":0.317,"Precursor.Area":617442.625,"Apex.RT..min.":15.02,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"TALNIPK","Annotated.Sequence":"[K].TALNIPK.[K]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P39517","Master.Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","Protein.Accessions":"P39517","Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.2727,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":378.73391,"Contaminant":"False","Aligned.m.z..Da.":378.73391,"MH...Da.":756.46054,"Theo..MH...Da.":756.46142,"DeltaM..ppm.":-1.16,"Deltam.z..Da.":-0.00044,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":4445892,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":80.42103,"Ion.Inject.Time..ms.":9.609,"RT..min.":9.0239,"First.Scan":7229,"Last.Scan":7229,"Master.Scan.s.":7217,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":94,"XCorr":1.21,"X..Protein.Groups":1,"Percolator.q.Value":0.00285,"Percolator.PEP":0.04283,"Percolator.SVMScore":0.246,"Precursor.Area":4194940.5,"Apex.RT..min.":9.03,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AB4)","Search.ID":"AB","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KVIGSFK","Annotated.Sequence":"[K].KVIGSFK.[D]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.2371,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":389.74488,"Contaminant":"False","Aligned.m.z..Da.":389.74486,"MH...Da.":778.48249,"Theo..MH...Da.":778.48215,"DeltaM..ppm.":0.43,"Deltam.z..Da.":0.00017,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1154875.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":58.95417,"Ion.Inject.Time..ms.":22,"RT..min.":6.2233,"First.Scan":3192,"Last.Scan":3192,"Master.Scan.s.":3191,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_03.raw","File.ID":"F9","Quan.Info":"","Peptides.Matched":22,"XCorr":1.94,"X..Protein.Groups":1,"Percolator.q.Value":0.00288,"Percolator.PEP":0.05061,"Percolator.SVMScore":0.209,"Precursor.Area":836048.0625,"Apex.RT..min.":6.21,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"TALNIPK","Annotated.Sequence":"[K].TALNIPK.[K]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P39517","Master.Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","Protein.Accessions":"P39517","Protein.Descriptions":"ATP-dependent RNA helicase DHH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DHH1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":3,"DeltaScore":0.1979,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":378.73391,"Contaminant":"False","Aligned.m.z..Da.":378.73391,"MH...Da.":756.46054,"Theo..MH...Da.":756.46142,"DeltaM..ppm.":-1.16,"Deltam.z..Da.":-0.00044,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":6099698.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":84.52002,"Ion.Inject.Time..ms.":5.692,"RT..min.":9.037,"First.Scan":7244,"Last.Scan":7244,"Master.Scan.s.":7243,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":94,"XCorr":0.96,"X..Protein.Groups":1,"Percolator.q.Value":0.00292,"Percolator.PEP":0.04374,"Percolator.SVMScore":0.242,"Precursor.Area":4194940.5,"Apex.RT..min.":9.03,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (L4)","Search.ID":"L","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"KSDEPSRESTPVR","Annotated.Sequence":"[K].KSDEPSREstPVR.[S]","Modifications":"S9(Phospho); T10(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q08421","Master.Protein.Descriptions":"Enhancer of translation termination 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ETT1 PE=1 SV=1","Protein.Accessions":"Q08421","Protein.Descriptions":"Enhancer of translation termination 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ETT1 PE=1 SV=1","X..Missed.Cleavages":2,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0031,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":549.89739,"Contaminant":"False","Aligned.m.z..Da.":549.89729,"MH...Da.":1647.67762,"Theo..MH...Da.":1647.67752,"DeltaM..ppm.":0.06,"Deltam.z..Da.":0.00003,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":18360136,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":17.37206,"Ion.Inject.Time..ms.":7.141,"RT..min.":4.5414,"First.Scan":1316,"Last.Scan":1316,"Master.Scan.s.":1309,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_01.raw","File.ID":"F25","Quan.Info":"","Peptides.Matched":988,"XCorr":3.19,"X..Protein.Groups":1,"Percolator.q.Value":0.00298,"Percolator.PEP":0.04153,"Percolator.SVMScore":0.369,"ptmRS..Binomial.Peptide.Score":216.76,"ptmRS..Isoform.Confidence.Probability":0.333,"ptmRS..Isoform.Group.Report":"y2+-H(3) O(4) P(12): 711.31; y2+-H(3) O(4) P(11): 667.79; y2+-H(3) O(4) P(10): 610.28; b1+- -2H(3) O(4) P -2H(3) O(4) P(10): 1081.49","ptmRS..Best.Site.Probabilities":"S6(Phospho): 66.67; S9(Phospho): 66.67; T10(Phospho): 66.67","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(6): 66.7; S(9): 66.7; T(10): 66.7"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"IQDPQMTGYVSTR","Annotated.Sequence":"[R].IQDPQmTGyVSTR.[Y]","Modifications":"M6(Oxidation); Y9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P32485","Master.Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","Protein.Accessions":"P32485","Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.2065,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":796.34485,"Contaminant":"False","Aligned.m.z..Da.":796.34485,"MH...Da.":1591.68241,"Theo..MH...Da.":1591.6822,"DeltaM..ppm.":0.13,"Deltam.z..Da.":0.00011,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":13501785,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":80.01498,"Ion.Inject.Time..ms.":22,"RT..min.":7.6447,"First.Scan":5416,"Last.Scan":5416,"Master.Scan.s.":5410,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":1211,"XCorr":0.92,"X..Protein.Groups":1,"Percolator.q.Value":0.003,"Percolator.PEP":0.04557,"Percolator.SVMScore":0.235,"Precursor.Area":12172759,"Apex.RT..min.":7.63,"ptmRS..Binomial.Peptide.Score":73.4,"ptmRS..Isoform.Confidence.Probability":0.25,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(9): 1032.45","ptmRS..Best.Site.Probabilities":"T7(Phospho): 25; Y9(Phospho): 25; S11(Phospho): 25; T12(Phospho): 25","ptmRS..Phospho.Site.Probabilities":"T(7): 25.0; Y(9): 25.0; S(11): 25.0; T(12): 25.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (G4)","Search.ID":"G","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"TLIGYGR","Annotated.Sequence":"[R].TLIGYGR.[F]","Modifications":"","X..Proteins":2,"Master.Protein.Accessions":"Q03558","Master.Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","Protein.Accessions":"Q03558; P41816","Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3; NADPH dehydrogenase 3 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE3 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.3161,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":390.22383,"Contaminant":"False","Aligned.m.z..Da.":390.22389,"MH...Da.":779.44039,"Theo..MH...Da.":779.44101,"DeltaM..ppm.":-0.81,"Deltam.z..Da.":-0.00031,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2633072.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":21.973,"Ion.Inject.Time..ms.":22,"RT..min.":9.0923,"First.Scan":7686,"Last.Scan":7686,"Master.Scan.s.":7677,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_06.raw","File.ID":"F30","Quan.Info":"","Peptides.Matched":76,"XCorr":1.55,"X..Protein.Groups":1,"Percolator.q.Value":0.00302,"Percolator.PEP":0.04473,"Percolator.SVMScore":0.309,"Precursor.Area":1830257.5,"Apex.RT..min.":9.09,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (R4)","Search.ID":"R","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].msEGITDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S2(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":3,"DeltaScore":0.1299,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1096.46816,"Contaminant":"False","Aligned.m.z..Da.":1096.468,"MH...Da.":2191.92905,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":0.55,"Deltam.z..Da.":0.0006,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":194856.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":92.82082,"Ion.Inject.Time..ms.":22,"RT..min.":16.1121,"First.Scan":15938,"Last.Scan":15938,"Master.Scan.s.":15935,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_01.raw","File.ID":"F19","Quan.Info":"","Peptides.Matched":1989,"XCorr":1.77,"X..Protein.Groups":1,"Percolator.q.Value":0.00317,"Percolator.PEP":0.05331,"Percolator.SVMScore":0.264,"Precursor.Area":11819790,"Apex.RT..min.":15.8,"ptmRS..Binomial.Peptide.Score":163.56,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(2): 112.04; b1+-H(3) O(4) P(4): 298.10; b1+-H(3) O(4) P(5): 411.19; b1+-H(3) O(4) P(6): 512.24; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35; b1+-H(3) O(4) P(9): 869.39","ptmRS..Best.Site.Probabilities":"S2(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 100.0; T(6): 0.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (X4)","Search.ID":"X","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ELDEIAASLENL","Annotated.Sequence":"[K].ELDEIAAsLENL.[-]","Modifications":"S8(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6777,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":698.8158,"Contaminant":"False","Aligned.m.z..Da.":698.81559,"MH...Da.":1396.62432,"Theo..MH...Da.":1396.62433,"DeltaM..ppm.":-0.01,"Deltam.z..Da.":0,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":701578.3125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":18.2389,"First.Scan":18224,"Last.Scan":18224,"Master.Scan.s.":18219,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_01.raw","File.ID":"F13","Quan.Info":"","Peptides.Matched":879,"XCorr":2.11,"X..Protein.Groups":1,"Percolator.q.Value":0.00318,"Percolator.PEP":0.0476,"Percolator.SVMScore":0.295,"Precursor.Area":675691.3125,"Apex.RT..min.":18.25,"ptmRS..Binomial.Peptide.Score":156.05,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"S8(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(8): 100.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (X4)","Search.ID":"X","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"EDGTTPFVAIWK","Annotated.Sequence":"[R].EDGTTPFVAIWK.[H]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.3482,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":682.34861,"Contaminant":"False","Aligned.m.z..Da.":682.34853,"MH...Da.":1363.68996,"Theo..MH...Da.":1363.68924,"DeltaM..ppm.":0.52,"Deltam.z..Da.":0.00036,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":203587.73438,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":36.17183,"Ion.Inject.Time..ms.":22,"RT..min.":16.9191,"First.Scan":16521,"Last.Scan":16521,"Master.Scan.s.":16509,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_01.raw","File.ID":"F13","Quan.Info":"","Peptides.Matched":713,"XCorr":1.12,"X..Protein.Groups":1,"Percolator.q.Value":0.00318,"Percolator.PEP":0.04387,"Percolator.SVMScore":0.313,"Precursor.Area":133602.71875,"Apex.RT..min.":16.91,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AC4)","Search.ID":"AC","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ILISTDLLAR","Annotated.Sequence":"[R].ILISTDLLAR.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.4956,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":557.84507,"Contaminant":"False","Aligned.m.z..Da.":557.84508,"MH...Da.":1114.68286,"Theo..MH...Da.":1114.68304,"DeltaM..ppm.":-0.15,"Deltam.z..Da.":-0.00009,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":233957.70312,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":35.78395,"Ion.Inject.Time..ms.":22,"RT..min.":15.6367,"First.Scan":15284,"Last.Scan":15284,"Master.Scan.s.":15283,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_02.raw","File.ID":"F8","Quan.Info":"","Peptides.Matched":124,"XCorr":1.13,"X..Protein.Groups":1,"Percolator.q.Value":0.0032,"Percolator.PEP":0.04661,"Percolator.SVMScore":0.215,"Precursor.Area":616202.875,"Apex.RT..min.":15.6,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (S4)","Search.ID":"S","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].msHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Acetyl); S2(Phospho); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":4,"Original.Precursor.Charge":4,"DeltaScore":0.0066,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":3,"m.z..Da.":778.55687,"Contaminant":"False","Aligned.m.z..Da.":778.55681,"MH...Da.":3111.20566,"Theo..MH...Da.":3111.20614,"DeltaM..ppm.":-0.16,"Deltam.z..Da.":-0.00012,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":964415.375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":1.79995,"Ion.Inject.Time..ms.":22,"RT..min.":9.5369,"First.Scan":8279,"Last.Scan":8279,"Master.Scan.s.":8270,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_06.raw","File.ID":"F18","Quan.Info":"","Peptides.Matched":751,"XCorr":1.51,"X..Protein.Groups":1,"Percolator.q.Value":0.00322,"Percolator.PEP":0.05297,"Percolator.SVMScore":0.199,"Precursor.Area":1819829.25,"Apex.RT..min.":9.54,"ptmRS..Binomial.Peptide.Score":109.46,"ptmRS..Isoform.Confidence.Probability":0.972,"ptmRS..Isoform.Group.Report":"b2+- -2H(3) O(4) P -2H(3) O(4) P(21): 1248.53","ptmRS..Best.Site.Probabilities":"S2(Phospho): 97.27; S13(Phospho): 99.91","ptmRS..Phospho.Site.Probabilities":"S(2): 97.3; Y(12): 2.8; S(13): 99.9; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"TLIGYGR","Annotated.Sequence":"[R].TLIGYGR.[F]","Modifications":"","X..Proteins":2,"Master.Protein.Accessions":"Q03558","Master.Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","Protein.Accessions":"Q03558; P41816","Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3; NADPH dehydrogenase 3 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE3 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.3043,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":390.22373,"Contaminant":"False","Aligned.m.z..Da.":390.22373,"MH...Da.":779.44018,"Theo..MH...Da.":779.44101,"DeltaM..ppm.":-1.07,"Deltam.z..Da.":-0.00042,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":8305415,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":54.4884,"Ion.Inject.Time..ms.":9.591,"RT..min.":8.9702,"First.Scan":7157,"Last.Scan":7157,"Master.Scan.s.":7152,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":76,"XCorr":1.61,"X..Protein.Groups":1,"Percolator.q.Value":0.00322,"Percolator.PEP":0.04951,"Percolator.SVMScore":0.219,"Precursor.Area":9035208,"Apex.RT..min.":8.98,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (N4)","Search.ID":"N","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSSPLHRLSPTGK","Annotated.Sequence":"[R].mSsPLHRLsPTGK.[S]","Modifications":"M1(Oxidation); S3(Phospho); S9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5115,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":793.84341,"Contaminant":"False","Aligned.m.z..Da.":793.84365,"MH...Da.":1586.67955,"Theo..MH...Da.":1586.67977,"DeltaM..ppm.":-0.14,"Deltam.z..Da.":-0.00011,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2040384.875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":64.98594,"Ion.Inject.Time..ms.":22,"RT..min.":7.7144,"First.Scan":5936,"Last.Scan":5936,"Master.Scan.s.":5931,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_05.raw","File.ID":"F23","Quan.Info":"","Peptides.Matched":988,"XCorr":1.31,"X..Protein.Groups":1,"Percolator.q.Value":0.00326,"Percolator.PEP":0.05218,"Percolator.SVMScore":0.25,"Precursor.Area":2279547.25,"Apex.RT..min.":7.72,"ptmRS..Binomial.Peptide.Score":118.55,"ptmRS..Isoform.Confidence.Probability":0.97,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(3): 304.10; b2+-H(3) O(4) P(6): 326.15; y2+- -2H(3) O(4) P -2H(3) O(4) P(11): 578.83","ptmRS..Best.Site.Probabilities":"S3(Phospho): 98.51; S9(Phospho): 98.51","ptmRS..Phospho.Site.Probabilities":"S(2): 1.5; S(3): 98.5; S(9): 98.5; T(11): 1.5"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Y4)","Search.ID":"Y","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"SFLTYIK","Annotated.Sequence":"[K].SFLTYIK.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6103,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":436.24821,"Contaminant":"False","Aligned.m.z..Da.":436.2482,"MH...Da.":871.48916,"Theo..MH...Da.":871.49238,"DeltaM..ppm.":-3.7,"Deltam.z..Da.":-0.00161,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1457785.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":13.7936,"First.Scan":13976,"Last.Scan":13976,"Master.Scan.s.":13971,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_06.raw","File.ID":"F12","Quan.Info":"","Peptides.Matched":310,"XCorr":1.36,"X..Protein.Groups":1,"Percolator.q.Value":0.00327,"Percolator.PEP":0.03722,"Percolator.SVMScore":0.318,"Precursor.Area":2721447.25,"Apex.RT..min.":13.81,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (M4)","Search.ID":"M","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].msHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Acetyl); S2(Phospho); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":4,"Original.Precursor.Charge":4,"DeltaScore":0.2,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":778.55692,"Contaminant":"False","Aligned.m.z..Da.":778.55694,"MH...Da.":3111.20586,"Theo..MH...Da.":3111.20614,"DeltaM..ppm.":-0.09,"Deltam.z..Da.":-0.00007,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1701914.125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":9.08551,"Ion.Inject.Time..ms.":22,"RT..min.":9.5397,"First.Scan":8279,"Last.Scan":8279,"Master.Scan.s.":8274,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_06.raw","File.ID":"F24","Quan.Info":"","Peptides.Matched":753,"XCorr":1.9,"X..Protein.Groups":1,"Percolator.q.Value":0.00328,"Percolator.PEP":0.06188,"Percolator.SVMScore":0.212,"Precursor.Area":1836263.875,"Apex.RT..min.":9.54,"ptmRS..Binomial.Peptide.Score":145.43,"ptmRS..Isoform.Confidence.Probability":0.5,"ptmRS..Isoform.Group.Report":"b2+- -2H(3) O(4) P -2H(3) O(4) P(21): 1248.53","ptmRS..Best.Site.Probabilities":"S2(Phospho): 50.01; Y12(Phospho): 50.01; S13(Phospho): 99.98","ptmRS..Phospho.Site.Probabilities":"S(2): 50.0; Y(12): 50.0; S(13): 100.0; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (K4)","Search.ID":"K","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSSPLHRLSPTGK","Annotated.Sequence":"[R].mSsPLHRLsPTGK.[S]","Modifications":"M1(Oxidation); S3(Phospho); S9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.3514,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":793.84385,"Contaminant":"False","Aligned.m.z..Da.":793.84398,"MH...Da.":1586.68043,"Theo..MH...Da.":1586.67977,"DeltaM..ppm.":0.42,"Deltam.z..Da.":0.00033,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2534899.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":70.7386,"Ion.Inject.Time..ms.":22,"RT..min.":7.7353,"First.Scan":5573,"Last.Scan":5573,"Master.Scan.s.":5568,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_02.raw","File.ID":"F26","Quan.Info":"","Peptides.Matched":979,"XCorr":1.11,"X..Protein.Groups":1,"Percolator.q.Value":0.00335,"Percolator.PEP":0.05143,"Percolator.SVMScore":0.272,"Precursor.Area":2393506.5,"Apex.RT..min.":7.74,"ptmRS..Binomial.Peptide.Score":118.55,"ptmRS..Isoform.Confidence.Probability":0.493,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(2): 217.06; b1+-H(3) O(4) P(3): 304.10; b2+-H(3) O(4) P(6): 326.15","ptmRS..Best.Site.Probabilities":"S2(Phospho): 50; S3(Phospho): 50; S9(Phospho): 98.51","ptmRS..Phospho.Site.Probabilities":"S(2): 50.0; S(3): 50.0; S(9): 98.5; T(11): 1.5"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (O4)","Search.ID":"O","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"VFDNIQR","Annotated.Sequence":"[R].VFDNIQR.[R]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.4491,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":446.23843,"Contaminant":"False","Aligned.m.z..Da.":446.23849,"MH...Da.":891.46959,"Theo..MH...Da.":891.46829,"DeltaM..ppm.":1.46,"Deltam.z..Da.":0.00065,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":5330338.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":29.84665,"Ion.Inject.Time..ms.":17.233,"RT..min.":8.2491,"First.Scan":6221,"Last.Scan":6221,"Master.Scan.s.":6218,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_04.raw","File.ID":"F22","Quan.Info":"","Peptides.Matched":363,"XCorr":2.16,"X..Protein.Groups":1,"Percolator.q.Value":0.00343,"Percolator.PEP":0.06328,"Percolator.SVMScore":0.212,"Precursor.Area":4143790,"Apex.RT..min.":8.26,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (G4)","Search.ID":"G","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].msHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss); S2(Phospho); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":4,"Original.Precursor.Charge":4,"DeltaScore":0.4175,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":735.29698,"Contaminant":"False","Aligned.m.z..Da.":735.29711,"MH...Da.":2938.1661,"Theo..MH...Da.":2938.15509,"DeltaM..ppm.":3.75,"Deltam.z..Da.":0.00275,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":298833.375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":26.26161,"Ion.Inject.Time..ms.":22,"RT..min.":15.0216,"First.Scan":15566,"Last.Scan":15566,"Master.Scan.s.":15554,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_06.raw","File.ID":"F30","Quan.Info":"","Peptides.Matched":1536,"XCorr":2.06,"X..Protein.Groups":1,"Percolator.q.Value":0.00348,"Percolator.PEP":0.05751,"Percolator.SVMScore":0.259,"ptmRS..Binomial.Peptide.Score":110.64,"ptmRS..Isoform.Confidence.Probability":0.333,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"S2(Phospho): 66.67; Y12(Phospho): 66.67; S13(Phospho): 66.67","ptmRS..Phospho.Site.Probabilities":"S(2): 66.7; Y(12): 66.7; S(13): 66.7; S(24): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Q4)","Search.ID":"Q","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"IQDPQMTGYVSTR","Annotated.Sequence":"[R].IQDPQMtGYVsTR.[Y]","Modifications":"T7(Phospho); S11(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P32485","Master.Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","Protein.Accessions":"P32485","Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.0422,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":828.32852,"Contaminant":"False","Aligned.m.z..Da.":828.32858,"MH...Da.":1655.64976,"Theo..MH...Da.":1655.65362,"DeltaM..ppm.":-2.33,"Deltam.z..Da.":-0.00193,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1567615.375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":67.09305,"Ion.Inject.Time..ms.":22,"RT..min.":9.905,"First.Scan":8180,"Last.Scan":8180,"Master.Scan.s.":8169,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_02.raw","File.ID":"F20","Quan.Info":"","Peptides.Matched":731,"XCorr":1.66,"X..Protein.Groups":1,"Percolator.q.Value":0.00354,"Percolator.PEP":0.08616,"Percolator.SVMScore":0.207,"Precursor.Area":1141086,"Apex.RT..min.":9.9,"ptmRS..Binomial.Peptide.Score":221.89,"ptmRS..Isoform.Confidence.Probability":0.004,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(11): 1316.53; y1+-H(3) O(4) P(10): 1201.51; y1+-H(3) O(4) P(8): 976.40; y1+-H(3) O(4) P(7): 845.36; y2+-H(3) O(4) P(10): 601.26; y1+(4): 462.27; y1+(3): 363.20","ptmRS..Best.Site.Probabilities":"T7(Phospho): 99.63; Y9(Phospho): 49.63; T12(Phospho): 50","ptmRS..Phospho.Site.Probabilities":"T(7): 99.6; Y(9): 49.6; S(11): 0.7; T(12): 50.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (R4)","Search.ID":"R","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KVIGSFK","Annotated.Sequence":"[K].KVIGSFK.[D]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.2757,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":389.74423,"Contaminant":"False","Aligned.m.z..Da.":389.74422,"MH...Da.":778.48119,"Theo..MH...Da.":778.48215,"DeltaM..ppm.":-1.23,"Deltam.z..Da.":-0.00048,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2810636.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":44.16944,"Ion.Inject.Time..ms.":22,"RT..min.":6.2108,"First.Scan":3104,"Last.Scan":3104,"Master.Scan.s.":3097,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_01.raw","File.ID":"F19","Quan.Info":"","Peptides.Matched":22,"XCorr":2.14,"X..Protein.Groups":1,"Percolator.q.Value":0.00355,"Percolator.PEP":0.05884,"Percolator.SVMScore":0.244,"Precursor.Area":2542738.25,"Apex.RT..min.":6.22,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (R4)","Search.ID":"R","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KSDEPSRESTPVR","Annotated.Sequence":"[K].KSDEPSREstPVR.[S]","Modifications":"S9(Phospho); T10(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q08421","Master.Protein.Descriptions":"Enhancer of translation termination 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ETT1 PE=1 SV=1","Protein.Accessions":"Q08421","Protein.Descriptions":"Enhancer of translation termination 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ETT1 PE=1 SV=1","X..Missed.Cleavages":2,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.036,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":549.89713,"Contaminant":"False","Aligned.m.z..Da.":549.89702,"MH...Da.":1647.67683,"Theo..MH...Da.":1647.67752,"DeltaM..ppm.":-0.42,"Deltam.z..Da.":-0.00023,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":29223504,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":11.33707,"Ion.Inject.Time..ms.":4.498,"RT..min.":4.5395,"First.Scan":1398,"Last.Scan":1398,"Master.Scan.s.":1394,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_01.raw","File.ID":"F19","Quan.Info":"","Peptides.Matched":971,"XCorr":3.33,"X..Protein.Groups":1,"Percolator.q.Value":0.00355,"Percolator.PEP":0.06023,"Percolator.SVMScore":0.239,"Precursor.Area":21254734,"Apex.RT..min.":4.54,"ptmRS..Binomial.Peptide.Score":193.01,"ptmRS..Isoform.Confidence.Probability":0.333,"ptmRS..Isoform.Group.Report":"y2+-H(3) O(4) P(12): 711.31; y2+-H(3) O(4) P(11): 667.79; y2+-H(3) O(4) P(10): 610.28","ptmRS..Best.Site.Probabilities":"S6(Phospho): 66.67; S9(Phospho): 66.67; T10(Phospho): 66.67","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(6): 66.7; S(9): 66.7; T(10): 66.7"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (N4)","Search.ID":"N","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"TLIGYGR","Annotated.Sequence":"[R].TLIGYGR.[F]","Modifications":"","X..Proteins":2,"Master.Protein.Accessions":"Q03558","Master.Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","Protein.Accessions":"Q03558; P41816","Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3; NADPH dehydrogenase 3 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE3 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.4118,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":390.22385,"Contaminant":"False","Aligned.m.z..Da.":390.22388,"MH...Da.":779.44043,"Theo..MH...Da.":779.44101,"DeltaM..ppm.":-0.75,"Deltam.z..Da.":-0.00029,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1847379.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":26.0424,"Ion.Inject.Time..ms.":22,"RT..min.":9.1507,"First.Scan":7805,"Last.Scan":7805,"Master.Scan.s.":7803,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_05.raw","File.ID":"F23","Quan.Info":"","Peptides.Matched":76,"XCorr":1.19,"X..Protein.Groups":1,"Percolator.q.Value":0.00357,"Percolator.PEP":0.06332,"Percolator.SVMScore":0.21,"Precursor.Area":1128379.25,"Apex.RT..min.":9.14,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (X4)","Search.ID":"X","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ELDEIAASLENL","Annotated.Sequence":"[K].ELDEIAASLENL.[-]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.4751,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":658.83302,"Contaminant":"False","Aligned.m.z..Da.":658.83277,"MH...Da.":1316.65876,"Theo..MH...Da.":1316.658,"DeltaM..ppm.":0.58,"Deltam.z..Da.":0.00038,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":703644.125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":19.6707,"Ion.Inject.Time..ms.":22,"RT..min.":18.4464,"First.Scan":18501,"Last.Scan":18501,"Master.Scan.s.":18492,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_01.raw","File.ID":"F13","Quan.Info":"","Peptides.Matched":669,"XCorr":1.81,"X..Protein.Groups":1,"Percolator.q.Value":0.00372,"Percolator.PEP":0.0593,"Percolator.SVMScore":0.246,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (K4)","Search.ID":"K","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"APQALMLAPTR","Annotated.Sequence":"[K].APQALMLAPTR.[E]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1154,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":584.83041,"Contaminant":"False","Aligned.m.z..Da.":584.83039,"MH...Da.":1168.65354,"Theo..MH...Da.":1168.65069,"DeltaM..ppm.":2.44,"Deltam.z..Da.":0.00143,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":770803,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":95.28053,"Ion.Inject.Time..ms.":22,"RT..min.":11.6051,"First.Scan":10399,"Last.Scan":10399,"Master.Scan.s.":10391,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_02.raw","File.ID":"F26","Quan.Info":"","Peptides.Matched":178,"XCorr":0.78,"X..Protein.Groups":1,"Percolator.q.Value":0.00373,"Percolator.PEP":0.05935,"Percolator.SVMScore":0.242,"Precursor.Area":877288.9375,"Apex.RT..min.":11.62,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (T4)","Search.ID":"T","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KSDEPSRESTPVR","Annotated.Sequence":"[K].KSDEPsREsTPVR.[S]","Modifications":"S6(Phospho); S9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q08421","Master.Protein.Descriptions":"Enhancer of translation termination 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ETT1 PE=1 SV=1","Protein.Accessions":"Q08421","Protein.Descriptions":"Enhancer of translation termination 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ETT1 PE=1 SV=1","X..Missed.Cleavages":2,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0167,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":549.89781,"Contaminant":"False","Aligned.m.z..Da.":549.89769,"MH...Da.":1647.67888,"Theo..MH...Da.":1647.67752,"DeltaM..ppm.":0.82,"Deltam.z..Da.":0.00045,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":11216664,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":19.36059,"Ion.Inject.Time..ms.":8.992,"RT..min.":4.5351,"First.Scan":1409,"Last.Scan":1409,"Master.Scan.s.":1405,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_05.raw","File.ID":"F17","Quan.Info":"","Peptides.Matched":1029,"XCorr":2.99,"X..Protein.Groups":1,"Percolator.q.Value":0.00378,"Percolator.PEP":0.05736,"Percolator.SVMScore":0.192,"Precursor.Area":17258928,"Apex.RT..min.":4.54,"ptmRS..Binomial.Peptide.Score":154.79,"ptmRS..Isoform.Confidence.Probability":0.333,"ptmRS..Isoform.Group.Report":"y2+-H(3) O(4) P(12): 711.31; y2+-H(3) O(4) P(11): 667.79; y2+-H(3) O(4) P(10): 610.28","ptmRS..Best.Site.Probabilities":"S6(Phospho): 66.67; S9(Phospho): 66.67; T10(Phospho): 66.67","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(6): 66.7; S(9): 66.7; T(10): 66.7"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (G4)","Search.ID":"G","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"TAVFYGGTPISK","Annotated.Sequence":"[K].TAVFYGGTPISK.[D]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.2037,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":620.83262,"Contaminant":"False","Aligned.m.z..Da.":620.83256,"MH...Da.":1240.65796,"Theo..MH...Da.":1240.65721,"DeltaM..ppm.":0.6,"Deltam.z..Da.":0.00037,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":949982.8125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":91.67433,"Ion.Inject.Time..ms.":22,"RT..min.":11.1642,"First.Scan":10514,"Last.Scan":10514,"Master.Scan.s.":10511,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_06.raw","File.ID":"F30","Quan.Info":"","Peptides.Matched":388,"XCorr":1.08,"X..Protein.Groups":1,"Percolator.q.Value":0.00386,"Percolator.PEP":0.06254,"Percolator.SVMScore":0.242,"Precursor.Area":875093.5625,"Apex.RT..min.":11.17,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (X4)","Search.ID":"X","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KVIGSFK","Annotated.Sequence":"[K].KVIGSFK.[D]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.2814,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":389.7444,"Contaminant":"False","Aligned.m.z..Da.":389.74442,"MH...Da.":778.48152,"Theo..MH...Da.":778.48215,"DeltaM..ppm.":-0.81,"Deltam.z..Da.":-0.00032,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1832966.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":37.25629,"Ion.Inject.Time..ms.":22,"RT..min.":6.1825,"First.Scan":3108,"Last.Scan":3108,"Master.Scan.s.":3102,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_01.raw","File.ID":"F13","Quan.Info":"","Peptides.Matched":22,"XCorr":1.99,"X..Protein.Groups":1,"Percolator.q.Value":0.00404,"Percolator.PEP":0.06212,"Percolator.SVMScore":0.236,"Precursor.Area":1547192.875,"Apex.RT..min.":6.18,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MFILDEADEMLSSGFK","Annotated.Sequence":"[K].mFILDEADEmLSSGFK.[E]","Modifications":"M1(Oxidation); M10(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":3,"DeltaScore":0.1684,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":932.92263,"Contaminant":"False","Aligned.m.z..Da.":932.92263,"MH...Da.":1864.83798,"Theo..MH...Da.":1864.83433,"DeltaM..ppm.":1.96,"Deltam.z..Da.":0.00182,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":438781.09375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":83.34643,"Ion.Inject.Time..ms.":22,"RT..min.":16.8962,"First.Scan":17424,"Last.Scan":17424,"Master.Scan.s.":17422,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":1871,"XCorr":0.95,"X..Protein.Groups":1,"Percolator.q.Value":0.00409,"Percolator.PEP":0.07937,"Percolator.SVMScore":0.127,"Precursor.Area":5900474,"Apex.RT..min.":16.81,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (H4)","Search.ID":"H","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"VFDNIQR","Annotated.Sequence":"[R].VFDNIQR.[R]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.3676,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":446.2381,"Contaminant":"False","Aligned.m.z..Da.":446.2382,"MH...Da.":891.46892,"Theo..MH...Da.":891.46829,"DeltaM..ppm.":0.7,"Deltam.z..Da.":0.00031,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":8756081,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":18.73741,"Ion.Inject.Time..ms.":11.956,"RT..min.":8.3749,"First.Scan":6800,"Last.Scan":6800,"Master.Scan.s.":6797,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_05.raw","File.ID":"F29","Quan.Info":"","Peptides.Matched":367,"XCorr":1.85,"X..Protein.Groups":1,"Percolator.q.Value":0.00411,"Percolator.PEP":0.06754,"Percolator.SVMScore":0.171,"Precursor.Area":8971945,"Apex.RT..min.":8.37,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (H4)","Search.ID":"H","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"KSDEPSRESTPVR","Annotated.Sequence":"[K].KSDEPSREstPVR.[S]","Modifications":"S9(Phospho); T10(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q08421","Master.Protein.Descriptions":"Enhancer of translation termination 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ETT1 PE=1 SV=1","Protein.Accessions":"Q08421","Protein.Descriptions":"Enhancer of translation termination 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ETT1 PE=1 SV=1","X..Missed.Cleavages":2,"Charge":3,"Original.Precursor.Charge":2,"DeltaScore":0.029,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":549.8972,"Contaminant":"False","Aligned.m.z..Da.":549.89708,"MH...Da.":1647.67703,"Theo..MH...Da.":1647.67752,"DeltaM..ppm.":-0.3,"Deltam.z..Da.":-0.00016,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":30226262,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":15.76892,"Ion.Inject.Time..ms.":4.747,"RT..min.":4.5409,"First.Scan":1378,"Last.Scan":1378,"Master.Scan.s.":1376,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_05.raw","File.ID":"F29","Quan.Info":"","Peptides.Matched":973,"XCorr":2.76,"X..Protein.Groups":1,"Percolator.q.Value":0.00411,"Percolator.PEP":0.06465,"Percolator.SVMScore":0.18,"Precursor.Area":28512714,"Apex.RT..min.":4.53,"ptmRS..Binomial.Peptide.Score":136.2,"ptmRS..Isoform.Confidence.Probability":0.333,"ptmRS..Isoform.Group.Report":"y2+-H(3) O(4) P(12): 711.31","ptmRS..Best.Site.Probabilities":"S6(Phospho): 66.67; S9(Phospho): 66.67; T10(Phospho): 66.67","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(6): 66.7; S(9): 66.7; T(10): 66.7"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (S4)","Search.ID":"S","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"IQDPQMTGYVSTR","Annotated.Sequence":"[R].IQDPQMtGYVsTR.[Y]","Modifications":"T7(Phospho); S11(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P32485","Master.Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","Protein.Accessions":"P32485","Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":3,"DeltaScore":0.0443,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":828.32689,"Contaminant":"False","Aligned.m.z..Da.":828.32684,"MH...Da.":1655.6465,"Theo..MH...Da.":1655.65362,"DeltaM..ppm.":-4.3,"Deltam.z..Da.":-0.00356,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":305214.4375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":82.22337,"Ion.Inject.Time..ms.":22,"RT..min.":9.8454,"First.Scan":8706,"Last.Scan":8706,"Master.Scan.s.":8699,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_06.raw","File.ID":"F18","Quan.Info":"","Peptides.Matched":677,"XCorr":1.58,"X..Protein.Groups":1,"Percolator.q.Value":0.0042,"Percolator.PEP":0.07032,"Percolator.SVMScore":0.137,"Precursor.Area":324596,"Apex.RT..min.":9.85,"ptmRS..Binomial.Peptide.Score":247.8,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(11): 1316.53; y1+-H(3) O(4) P(10): 1201.51; y1+-H(3) O(4) P(8): 976.40; y1+-H(3) O(4) P(7): 845.36; y2+-H(3) O(4) P(10): 601.26; y1+(4): 462.27; y1+(3): 363.20","ptmRS..Best.Site.Probabilities":"T7(Phospho): 99.71; Y9(Phospho): 49.85; T12(Phospho): 50.15","ptmRS..Phospho.Site.Probabilities":"T(7): 99.7; Y(9): 49.9; S(11): 0.3; T(12): 50.2"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AC4)","Search.ID":"AC","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KSDEPSRESTPVR","Annotated.Sequence":"[K].KSDEPSREstPVR.[S]","Modifications":"S9(Phospho); T10(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q08421","Master.Protein.Descriptions":"Enhancer of translation termination 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ETT1 PE=1 SV=1","Protein.Accessions":"Q08421","Protein.Descriptions":"Enhancer of translation termination 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ETT1 PE=1 SV=1","X..Missed.Cleavages":2,"Charge":3,"Original.Precursor.Charge":2,"DeltaScore":0.0113,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":549.8975,"Contaminant":"False","Aligned.m.z..Da.":549.89739,"MH...Da.":1647.67796,"Theo..MH...Da.":1647.67752,"DeltaM..ppm.":0.26,"Deltam.z..Da.":0.00014,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":24731220,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":14.95609,"Ion.Inject.Time..ms.":5.537,"RT..min.":4.5543,"First.Scan":1363,"Last.Scan":1363,"Master.Scan.s.":1361,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_02.raw","File.ID":"F8","Quan.Info":"","Peptides.Matched":986,"XCorr":2.66,"X..Protein.Groups":1,"Percolator.q.Value":0.00423,"Percolator.PEP":0.06558,"Percolator.SVMScore":0.15,"Precursor.Area":12137978,"Apex.RT..min.":4.55,"ptmRS..Binomial.Peptide.Score":137.32,"ptmRS..Isoform.Confidence.Probability":0.333,"ptmRS..Isoform.Group.Report":"y2+-H(3) O(4) P(12): 711.31; y2+-H(3) O(4) P(10): 610.28","ptmRS..Best.Site.Probabilities":"S6(Phospho): 66.67; S9(Phospho): 66.67; T10(Phospho): 66.67","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(6): 66.7; S(9): 66.7; T(10): 66.7"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (J4)","Search.ID":"J","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSSPLHRLSPTGK","Annotated.Sequence":"[R].mSsPLHRLsPTGK.[S]","Modifications":"M1(Oxidation); S3(Phospho); S9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P33419","Master.Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","Protein.Accessions":"P33419","Protein.Descriptions":"Spindle pole component 29 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPC29 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.4375,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":793.84333,"Contaminant":"False","Aligned.m.z..Da.":793.84333,"MH...Da.":1586.67938,"Theo..MH...Da.":1586.67977,"DeltaM..ppm.":-0.24,"Deltam.z..Da.":-0.00019,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2429249.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":71.41531,"Ion.Inject.Time..ms.":22,"RT..min.":7.7201,"First.Scan":5560,"Last.Scan":5560,"Master.Scan.s.":5557,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_03.raw","File.ID":"F27","Quan.Info":"","Peptides.Matched":1026,"XCorr":1.12,"X..Protein.Groups":1,"Percolator.q.Value":0.00426,"Percolator.PEP":0.07625,"Percolator.SVMScore":0.159,"Precursor.Area":2640847.25,"Apex.RT..min.":7.73,"ptmRS..Binomial.Peptide.Score":85.98,"ptmRS..Isoform.Confidence.Probability":0.965,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(3): 304.10; y2+- -2H(3) O(4) P -2H(3) O(4) P(11): 578.83","ptmRS..Best.Site.Probabilities":"S3(Phospho): 98.21; S9(Phospho): 98.21","ptmRS..Phospho.Site.Probabilities":"S(2): 1.8; S(3): 98.2; S(9): 98.2; T(11): 1.8"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (M4)","Search.ID":"M","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"IQDPQMTGYVSTR","Annotated.Sequence":"[R].IQDPQmtGYVsTR.[Y]","Modifications":"M6(Oxidation); T7(Phospho); S11(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P32485","Master.Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","Protein.Accessions":"P32485","Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":3,"DeltaScore":0.0811,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":836.32625,"Contaminant":"False","Aligned.m.z..Da.":836.32631,"MH...Da.":1671.64522,"Theo..MH...Da.":1671.64853,"DeltaM..ppm.":-1.98,"Deltam.z..Da.":-0.00165,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2648616.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":84.42307,"Ion.Inject.Time..ms.":22,"RT..min.":7.7003,"First.Scan":5820,"Last.Scan":5820,"Master.Scan.s.":5817,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_06.raw","File.ID":"F24","Quan.Info":"","Peptides.Matched":708,"XCorr":1.85,"X..Protein.Groups":1,"Percolator.q.Value":0.00429,"Percolator.PEP":0.08227,"Percolator.SVMScore":0.154,"Precursor.Area":2725992.75,"Apex.RT..min.":7.71,"ptmRS..Binomial.Peptide.Score":200.84,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(11): 1332.53; y1+-H(3) O(4) P(10): 1217.50; y1+-H(3) O(4) P(8): 992.39; y2+-H(3) O(4) P(10): 609.25; y1+(4): 462.27; y1+(3): 363.20; y1+(2): 276.17","ptmRS..Best.Site.Probabilities":"T7(Phospho): 99.26; Y9(Phospho): 98.53","ptmRS..Phospho.Site.Probabilities":"T(7): 99.3; Y(9): 98.5; S(11): 0.7; T(12): 1.5"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AA4)","Search.ID":"AA","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ELDEIAASLENL","Annotated.Sequence":"[K].ELDEIAAsLENL.[-]","Modifications":"S8(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6946,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":698.81581,"Contaminant":"False","Aligned.m.z..Da.":698.81615,"MH...Da.":1396.62435,"Theo..MH...Da.":1396.62433,"DeltaM..ppm.":0.01,"Deltam.z..Da.":1e-05,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":497543.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":18.2418,"First.Scan":18591,"Last.Scan":18591,"Master.Scan.s.":18585,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_04.raw","File.ID":"F10","Quan.Info":"","Peptides.Matched":879,"XCorr":2.03,"X..Protein.Groups":1,"Percolator.q.Value":0.00431,"Percolator.PEP":0.08154,"Percolator.SVMScore":0.138,"ptmRS..Binomial.Peptide.Score":136.34,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"S8(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(8): 100.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Y4)","Search.ID":"Y","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DIFSNDELLSDAYDAK","Annotated.Sequence":"[K].DIFSNDELLSDAYDAK.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.2941,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":605.9472,"Contaminant":"False","Aligned.m.z..Da.":605.94704,"MH...Da.":1815.82704,"Theo..MH...Da.":1815.82831,"DeltaM..ppm.":-0.7,"Deltam.z..Da.":-0.00042,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":705046.875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":17.1268,"First.Scan":17456,"Last.Scan":17456,"Master.Scan.s.":17449,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_06.raw","File.ID":"F12","Quan.Info":"","Peptides.Matched":1656,"XCorr":1.19,"X..Protein.Groups":1,"Percolator.q.Value":0.00434,"Percolator.PEP":0.05166,"Percolator.SVMScore":0.244,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (W4)","Search.ID":"W","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ILISTDLLAR","Annotated.Sequence":"[R].ILISTDLLAR.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.4343,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":557.84546,"Contaminant":"False","Aligned.m.z..Da.":557.84542,"MH...Da.":1114.68365,"Theo..MH...Da.":1114.68304,"DeltaM..ppm.":0.55,"Deltam.z..Da.":0.00031,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":203864.46875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":59.51428,"Ion.Inject.Time..ms.":22,"RT..min.":15.6518,"First.Scan":15512,"Last.Scan":15512,"Master.Scan.s.":15510,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_02.raw","File.ID":"F14","Quan.Info":"","Peptides.Matched":124,"XCorr":0.99,"X..Protein.Groups":1,"Percolator.q.Value":0.00447,"Percolator.PEP":0.0793,"Percolator.SVMScore":0.176,"Precursor.Area":1168102.5,"Apex.RT..min.":15.61,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (R4)","Search.ID":"R","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"IQDPQMTGYVSTR","Annotated.Sequence":"[R].IQDPQMtGYVsTR.[Y]","Modifications":"T7(Phospho); S11(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P32485","Master.Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","Protein.Accessions":"P32485","Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.0435,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":828.32831,"Contaminant":"False","Aligned.m.z..Da.":828.32831,"MH...Da.":1655.64934,"Theo..MH...Da.":1655.65362,"DeltaM..ppm.":-2.58,"Deltam.z..Da.":-0.00214,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1803148.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":70.55479,"Ion.Inject.Time..ms.":22,"RT..min.":9.9067,"First.Scan":8138,"Last.Scan":8138,"Master.Scan.s.":8128,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_01.raw","File.ID":"F19","Quan.Info":"","Peptides.Matched":748,"XCorr":1.61,"X..Protein.Groups":1,"Percolator.q.Value":0.00447,"Percolator.PEP":0.1032,"Percolator.SVMScore":0.127,"Precursor.Area":1336555.375,"Apex.RT..min.":9.9,"ptmRS..Binomial.Peptide.Score":228.63,"ptmRS..Isoform.Confidence.Probability":0.006,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(11): 1316.53; y1+-H(3) O(4) P(10): 1201.51; y1+-H(3) O(4) P(8): 976.40; y1+-H(3) O(4) P(7): 845.36; y2+-H(3) O(4) P(10): 601.26; y1+(4): 462.27; y1+(3): 363.20","ptmRS..Best.Site.Probabilities":"T7(Phospho): 100; Y9(Phospho): 98.7","ptmRS..Phospho.Site.Probabilities":"T(7): 100.0; Y(9): 98.7; S(11): 0.7; T(12): 0.7"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (A4)","Search.ID":"A","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].mSEGItDIEEsQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss); T6(Phospho); S11(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0966,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":743.96379,"Contaminant":"False","Aligned.m.z..Da.":743.96371,"MH...Da.":2229.87683,"Theo..MH...Da.":2229.88361,"DeltaM..ppm.":-3.04,"Deltam.z..Da.":-0.00226,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":798762.875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":15.7126,"First.Scan":15729,"Last.Scan":15729,"Master.Scan.s.":15724,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_03.raw","File.ID":"F36","Quan.Info":"","Peptides.Matched":1065,"XCorr":1.45,"X..Protein.Groups":1,"Percolator.q.Value":0.00475,"Percolator.PEP":0.125,"Percolator.SVMScore":0.144,"Precursor.Area":844525.25,"Apex.RT..min.":15.72,"ptmRS..Binomial.Peptide.Score":133.77,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"b2+-H(3) O(4) P(9): 454.18; b2+-H(3) O(4) P(10): 518.70","ptmRS..Best.Site.Probabilities":"S2(Phospho): 99.97; T6(Phospho): 99.97","ptmRS..Phospho.Site.Probabilities":"S(2): 100.0; T(6): 100.0; S(11): 0.1; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (M4)","Search.ID":"M","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"FDDMELDENLLR","Annotated.Sequence":"[K].FDDmELDENLLR.[G]","Modifications":"M4(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":4,"DeltaScore":0.3514,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":763.34303,"Contaminant":"False","Aligned.m.z..Da.":763.34315,"MH...Da.":1525.67877,"Theo..MH...Da.":1525.6839,"DeltaM..ppm.":-3.36,"Deltam.z..Da.":-0.00256,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":83512.24219,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":94.31798,"Ion.Inject.Time..ms.":22,"RT..min.":14.5752,"First.Scan":14917,"Last.Scan":14917,"Master.Scan.s.":14911,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_06.raw","File.ID":"F24","Quan.Info":"","Peptides.Matched":1024,"XCorr":1.11,"X..Protein.Groups":1,"Percolator.q.Value":0.00499,"Percolator.PEP":0.09278,"Percolator.SVMScore":0.128,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AB4)","Search.ID":"AB","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LEAGLSDSK","Annotated.Sequence":"[K].LEAGLsDSK.[Q]","Modifications":"S6(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1015,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":500.22449,"Contaminant":"False","Aligned.m.z..Da.":500.22451,"MH...Da.":999.4417,"Theo..MH...Da.":999.43943,"DeltaM..ppm.":2.27,"Deltam.z..Da.":0.00113,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1786139.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":44.85044,"Ion.Inject.Time..ms.":22,"RT..min.":7.5989,"First.Scan":5432,"Last.Scan":5432,"Master.Scan.s.":5427,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_03.raw","File.ID":"F9","Quan.Info":"","Peptides.Matched":672,"XCorr":1.97,"X..Protein.Groups":1,"Percolator.q.Value":0.00511,"Percolator.PEP":0.09253,"Percolator.SVMScore":0.088,"Precursor.Area":1408796.25,"Apex.RT..min.":7.59,"ptmRS..Binomial.Peptide.Score":137.09,"ptmRS..Isoform.Confidence.Probability":0.992,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(8): 788.38; y1+-H(3) O(4) P(7): 659.34; y1+-H(3) O(4) P(6): 588.30; y1+-H(3) O(4) P(4): 418.19; y1+(2): 234.14","ptmRS..Best.Site.Probabilities":"S6(Phospho): 99.16","ptmRS..Phospho.Site.Probabilities":"S(6): 99.2; S(8): 0.8"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSHEGEEDLLEYSDNEQEIQIDASK","Annotated.Sequence":"[-].mSHEGEEDLLEYsDNEQEIQIDASK.[A]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S13(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":2,"DeltaScore":0.1455,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":967.40304,"Contaminant":"False","Aligned.m.z..Da.":967.40304,"MH...Da.":2900.19455,"Theo..MH...Da.":2900.19933,"DeltaM..ppm.":-1.65,"Deltam.z..Da.":-0.00159,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":479359.625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":78.97038,"Ion.Inject.Time..ms.":22,"RT..min.":17.5935,"First.Scan":18266,"Last.Scan":18266,"Master.Scan.s.":18259,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":2262,"XCorr":1.1,"X..Protein.Groups":1,"Percolator.q.Value":0.00518,"Percolator.PEP":0.08454,"Percolator.SVMScore":0.137,"ptmRS..Binomial.Peptide.Score":71.94,"ptmRS..Isoform.Confidence.Probability":0.492,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"Y12(Phospho): 49.19; S13(Phospho): 49.19","ptmRS..Phospho.Site.Probabilities":"S(2): 1.6; Y(12): 49.2; S(13): 49.2; S(24): 0.1"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (M4)","Search.ID":"M","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"TLIGYGR","Annotated.Sequence":"[R].TLIGYGR.[F]","Modifications":"","X..Proteins":2,"Master.Protein.Accessions":"Q03558","Master.Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","Protein.Accessions":"Q03558; P41816","Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3; NADPH dehydrogenase 3 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE3 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.3664,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":390.22381,"Contaminant":"False","Aligned.m.z..Da.":390.22382,"MH...Da.":779.44035,"Theo..MH...Da.":779.44101,"DeltaM..ppm.":-0.85,"Deltam.z..Da.":-0.00033,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1754168.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":27.28308,"Ion.Inject.Time..ms.":22,"RT..min.":9.086,"First.Scan":7675,"Last.Scan":7675,"Master.Scan.s.":7663,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_06.raw","File.ID":"F24","Quan.Info":"","Peptides.Matched":76,"XCorr":1.31,"X..Protein.Groups":1,"Percolator.q.Value":0.00519,"Percolator.PEP":0.09484,"Percolator.SVMScore":0.124,"Precursor.Area":1200785.75,"Apex.RT..min.":9.09,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (S4)","Search.ID":"S","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"IQDPQMTGYVSTR","Annotated.Sequence":"[R].IQDPQmtGYVsTR.[Y]","Modifications":"M6(Oxidation); T7(Phospho); S11(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P32485","Master.Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","Protein.Accessions":"P32485","Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":3,"DeltaScore":0.0524,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":836.32774,"Contaminant":"False","Aligned.m.z..Da.":836.32782,"MH...Da.":1671.64821,"Theo..MH...Da.":1671.64853,"DeltaM..ppm.":-0.19,"Deltam.z..Da.":-0.00016,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1699824.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":76.69752,"Ion.Inject.Time..ms.":22,"RT..min.":7.7139,"First.Scan":5817,"Last.Scan":5817,"Master.Scan.s.":5813,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_06.raw","File.ID":"F18","Quan.Info":"","Peptides.Matched":764,"XCorr":1.91,"X..Protein.Groups":1,"Percolator.q.Value":0.00529,"Percolator.PEP":0.08724,"Percolator.SVMScore":0.089,"Precursor.Area":1215691.625,"Apex.RT..min.":7.71,"ptmRS..Binomial.Peptide.Score":162.93,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(11): 1332.53; y1+-H(3) O(4) P(10): 1217.50; y1+-H(3) O(4) P(8): 992.39; y2+-H(3) O(4) P(10): 609.25; y1+(4): 462.27; y1+(3): 363.20","ptmRS..Best.Site.Probabilities":"T7(Phospho): 98.37; Y9(Phospho): 98.37","ptmRS..Phospho.Site.Probabilities":"T(7): 98.4; Y(9): 98.4; S(11): 0.8; T(12): 2.4"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (S4)","Search.ID":"S","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KVIGSFK","Annotated.Sequence":"[K].KVIGSFK.[D]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.28,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":389.74401,"Contaminant":"False","Aligned.m.z..Da.":389.74401,"MH...Da.":778.48076,"Theo..MH...Da.":778.48215,"DeltaM..ppm.":-1.79,"Deltam.z..Da.":-0.0007,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1569597.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":38.93098,"Ion.Inject.Time..ms.":22,"RT..min.":6.1726,"First.Scan":3549,"Last.Scan":3549,"Master.Scan.s.":3538,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_06.raw","File.ID":"F18","Quan.Info":"","Peptides.Matched":22,"XCorr":2,"X..Protein.Groups":1,"Percolator.q.Value":0.00529,"Percolator.PEP":0.08562,"Percolator.SVMScore":0.093,"Precursor.Area":1650762.875,"Apex.RT..min.":6.18,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (N4)","Search.ID":"N","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"KSDEPSRESTPVR","Annotated.Sequence":"[K].KSDEPSREstPVR.[S]","Modifications":"S9(Phospho); T10(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q08421","Master.Protein.Descriptions":"Enhancer of translation termination 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ETT1 PE=1 SV=1","Protein.Accessions":"Q08421","Protein.Descriptions":"Enhancer of translation termination 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ETT1 PE=1 SV=1","X..Missed.Cleavages":2,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0206,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":549.89805,"Contaminant":"False","Aligned.m.z..Da.":549.898,"MH...Da.":1647.67961,"Theo..MH...Da.":1647.67752,"DeltaM..ppm.":1.27,"Deltam.z..Da.":0.0007,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":17189464,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":16.78776,"Ion.Inject.Time..ms.":6.016,"RT..min.":4.5075,"First.Scan":1347,"Last.Scan":1347,"Master.Scan.s.":1342,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_05.raw","File.ID":"F23","Quan.Info":"","Peptides.Matched":1066,"XCorr":2.91,"X..Protein.Groups":1,"Percolator.q.Value":0.00533,"Percolator.PEP":0.1086,"Percolator.SVMScore":0.094,"Precursor.Area":22912298,"Apex.RT..min.":4.52,"ptmRS..Binomial.Peptide.Score":174.95,"ptmRS..Isoform.Confidence.Probability":0.333,"ptmRS..Isoform.Group.Report":"y2+-H(3) O(4) P(12): 711.31; y2+-H(3) O(4) P(11): 667.79; y2+-H(3) O(4) P(10): 610.28","ptmRS..Best.Site.Probabilities":"S6(Phospho): 66.67; S9(Phospho): 66.67; T10(Phospho): 66.67","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(6): 66.7; S(9): 66.7; T(10): 66.7"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Z4)","Search.ID":"Z","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"SFLTYIK","Annotated.Sequence":"[K].SFLTYIK.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6294,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":436.24854,"Contaminant":"False","Aligned.m.z..Da.":436.24854,"MH...Da.":871.48981,"Theo..MH...Da.":871.49238,"DeltaM..ppm.":-2.95,"Deltam.z..Da.":-0.00129,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":788640.125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":13.6819,"First.Scan":13210,"Last.Scan":13210,"Master.Scan.s.":13207,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_05.raw","File.ID":"F11","Quan.Info":"","Peptides.Matched":310,"XCorr":1.43,"X..Protein.Groups":1,"Percolator.q.Value":0.00534,"Percolator.PEP":0.09241,"Percolator.SVMScore":0.126,"Precursor.Area":2033588.875,"Apex.RT..min.":13.71,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AC4)","Search.ID":"AC","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"VFDNIQR","Annotated.Sequence":"[R].VFDNIQR.[R]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.3413,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":446.23944,"Contaminant":"False","Aligned.m.z..Da.":446.23948,"MH...Da.":891.4716,"Theo..MH...Da.":891.46829,"DeltaM..ppm.":3.72,"Deltam.z..Da.":0.00166,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1142055.375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":69.0407,"Ion.Inject.Time..ms.":22,"RT..min.":8.2588,"First.Scan":6175,"Last.Scan":6175,"Master.Scan.s.":6171,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_02.raw","File.ID":"F8","Quan.Info":"","Peptides.Matched":407,"XCorr":1.67,"X..Protein.Groups":1,"Percolator.q.Value":0.00538,"Percolator.PEP":0.09043,"Percolator.SVMScore":0.087,"Precursor.Area":1157289.75,"Apex.RT..min.":8.27,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (R4)","Search.ID":"R","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ILISTDLLAR","Annotated.Sequence":"[R].ILISTDLLAR.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.3579,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":557.84632,"Contaminant":"False","Aligned.m.z..Da.":557.84629,"MH...Da.":1114.68536,"Theo..MH...Da.":1114.68304,"DeltaM..ppm.":2.09,"Deltam.z..Da.":0.00116,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":197450.0625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":46.18043,"Ion.Inject.Time..ms.":22,"RT..min.":15.6535,"First.Scan":15377,"Last.Scan":15377,"Master.Scan.s.":15376,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_01.raw","File.ID":"F19","Quan.Info":"","Peptides.Matched":126,"XCorr":0.95,"X..Protein.Groups":1,"Percolator.q.Value":0.0056,"Percolator.PEP":0.1398,"Percolator.SVMScore":0.062,"Precursor.Area":1218944.625,"Apex.RT..min.":15.61,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (T4)","Search.ID":"T","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LEAGLSDSK","Annotated.Sequence":"[K].LEAGLsDSK.[Q]","Modifications":"S6(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":3,"DeltaScore":0.0282,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":500.22286,"Contaminant":"False","Aligned.m.z..Da.":500.22293,"MH...Da.":999.43845,"Theo..MH...Da.":999.43943,"DeltaM..ppm.":-0.99,"Deltam.z..Da.":-0.00049,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":709300.1875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":86.32841,"Ion.Inject.Time..ms.":22,"RT..min.":7.6797,"First.Scan":5741,"Last.Scan":5741,"Master.Scan.s.":5734,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_05.raw","File.ID":"F17","Quan.Info":"","Peptides.Matched":602,"XCorr":1.77,"X..Protein.Groups":1,"Percolator.q.Value":0.00578,"Percolator.PEP":0.09576,"Percolator.SVMScore":0.084,"Precursor.Area":881873.875,"Apex.RT..min.":7.7,"ptmRS..Binomial.Peptide.Score":128.06,"ptmRS..Isoform.Confidence.Probability":0.5,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(8): 788.38; y1+-H(3) O(4) P(7): 659.34; y1+(2): 234.14","ptmRS..Best.Site.Probabilities":"S6(Phospho): 50; S8(Phospho): 50","ptmRS..Phospho.Site.Probabilities":"S(6): 50.0; S(8): 50.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"LEAESWQEK","Annotated.Sequence":"[K].LEAESWQEK.[Y]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":3,"DeltaScore":0.0806,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":560.27071,"Contaminant":"False","Aligned.m.z..Da.":560.27071,"MH...Da.":1119.53414,"Theo..MH...Da.":1119.53168,"DeltaM..ppm.":2.2,"Deltam.z..Da.":0.00123,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":994242.0625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":93.94165,"Ion.Inject.Time..ms.":16.283,"RT..min.":8.4143,"First.Scan":6428,"Last.Scan":6428,"Master.Scan.s.":6424,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":495,"XCorr":1.24,"X..Protein.Groups":1,"Percolator.q.Value":0.00581,"Percolator.PEP":0.1151,"Percolator.SVMScore":0.054,"Precursor.Area":2601225,"Apex.RT..min.":8.39,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (M4)","Search.ID":"M","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"TGTFSIAALQR","Annotated.Sequence":"[K].TGTFSIAALQR.[I]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":3,"DeltaScore":0.2347,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":582.82077,"Contaminant":"False","Aligned.m.z..Da.":582.82086,"MH...Da.":1164.63426,"Theo..MH...Da.":1164.63715,"DeltaM..ppm.":-2.48,"Deltam.z..Da.":-0.00145,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":311741.3125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":75.60873,"Ion.Inject.Time..ms.":22,"RT..min.":13.439,"First.Scan":13533,"Last.Scan":13533,"Master.Scan.s.":13526,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_06.raw","File.ID":"F24","Quan.Info":"","Peptides.Matched":368,"XCorr":0.98,"X..Protein.Groups":1,"Percolator.q.Value":0.00587,"Percolator.PEP":0.109,"Percolator.SVMScore":0.094,"Precursor.Area":2726012.25,"Apex.RT..min.":13.4,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"KSDEPSRESTPVR","Annotated.Sequence":"[K].KSDEPSREstPVR.[S]","Modifications":"S9(Phospho); T10(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q08421","Master.Protein.Descriptions":"Enhancer of translation termination 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ETT1 PE=1 SV=1","Protein.Accessions":"Q08421","Protein.Descriptions":"Enhancer of translation termination 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ETT1 PE=1 SV=1","X..Missed.Cleavages":2,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0104,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":549.89671,"Contaminant":"False","Aligned.m.z..Da.":549.89673,"MH...Da.":1647.67557,"Theo..MH...Da.":1647.67752,"DeltaM..ppm.":-1.18,"Deltam.z..Da.":-0.00065,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":52978368,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":21.35139,"Ion.Inject.Time..ms.":2.448,"RT..min.":4.5693,"First.Scan":1583,"Last.Scan":1583,"Master.Scan.s.":1580,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":963,"XCorr":2.88,"X..Protein.Groups":1,"Percolator.q.Value":0.0059,"Percolator.PEP":0.1017,"Percolator.SVMScore":0.101,"Precursor.Area":54857440,"Apex.RT..min.":4.58,"ptmRS..Binomial.Peptide.Score":185.57,"ptmRS..Isoform.Confidence.Probability":0.333,"ptmRS..Isoform.Group.Report":"y2+-H(3) O(4) P(12): 711.31; y2+-H(3) O(4) P(11): 667.79; y2+-H(3) O(4) P(10): 610.28; b2+- -2H(3) O(4) P -2H(3) O(4) P(10): 541.25","ptmRS..Best.Site.Probabilities":"S6(Phospho): 66.67; S9(Phospho): 66.67; T10(Phospho): 66.67","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(6): 66.7; S(9): 66.7; T(10): 66.7"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (T4)","Search.ID":"T","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"VFDNIQR","Annotated.Sequence":"[R].VFDNIQR.[R]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":3,"DeltaScore":0.2556,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":446.238,"Contaminant":"False","Aligned.m.z..Da.":446.23803,"MH...Da.":891.46872,"Theo..MH...Da.":891.46829,"DeltaM..ppm.":0.48,"Deltam.z..Da.":0.00021,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":437838.84375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":76.86365,"Ion.Inject.Time..ms.":22,"RT..min.":8.391,"First.Scan":6783,"Last.Scan":6783,"Master.Scan.s.":6774,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_05.raw","File.ID":"F17","Quan.Info":"","Peptides.Matched":401,"XCorr":1.33,"X..Protein.Groups":1,"Percolator.q.Value":0.0059,"Percolator.PEP":0.102,"Percolator.SVMScore":0.07,"Precursor.Area":2336335.25,"Apex.RT..min.":8.35,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (V4)","Search.ID":"V","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"SFLTYIK","Annotated.Sequence":"[K].SFLTYIK.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":1,"Original.Precursor.Charge":2,"DeltaScore":0.6344,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":871.49007,"Contaminant":"False","Aligned.m.z..Da.":871.49015,"MH...Da.":871.49007,"Theo..MH...Da.":871.49238,"DeltaM..ppm.":-2.65,"Deltam.z..Da.":-0.00231,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":410373.78125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":72.43655,"Ion.Inject.Time..ms.":22,"RT..min.":13.7098,"First.Scan":13183,"Last.Scan":13183,"Master.Scan.s.":13174,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_03.raw","File.ID":"F15","Quan.Info":"","Peptides.Matched":272,"XCorr":1.86,"X..Protein.Groups":1,"Percolator.q.Value":0.00607,"Percolator.PEP":0.1268,"Percolator.SVMScore":0.057,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"TLIGYGR","Annotated.Sequence":"[R].TLIGYGR.[F]","Modifications":"","X..Proteins":2,"Master.Protein.Accessions":"Q03558","Master.Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","Protein.Accessions":"Q03558; P41816","Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3; NADPH dehydrogenase 3 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE3 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.3152,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":390.22363,"Contaminant":"False","Aligned.m.z..Da.":390.22366,"MH...Da.":779.43999,"Theo..MH...Da.":779.44101,"DeltaM..ppm.":-1.31,"Deltam.z..Da.":-0.00051,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":3932547.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":35.52353,"Ion.Inject.Time..ms.":22,"RT..min.":8.9788,"First.Scan":7190,"Last.Scan":7190,"Master.Scan.s.":7183,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":76,"XCorr":1.65,"X..Protein.Groups":1,"Percolator.q.Value":0.00616,"Percolator.PEP":0.1084,"Percolator.SVMScore":0.088,"Precursor.Area":3078892.75,"Apex.RT..min.":8.98,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (L4)","Search.ID":"L","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DFLLKPELSR","Annotated.Sequence":"[K].DFLLKPELSR.[A]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"Q07478","Master.Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","Protein.Accessions":"Q07478","Protein.Descriptions":"ATP-dependent RNA helicase SUB2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUB2 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6457,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":609.34897,"Contaminant":"False","Aligned.m.z..Da.":609.34909,"MH...Da.":1217.69068,"Theo..MH...Da.":1217.68885,"DeltaM..ppm.":1.5,"Deltam.z..Da.":0.00091,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":595846.1875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":13.1609,"First.Scan":12380,"Last.Scan":12380,"Master.Scan.s.":12372,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_01.raw","File.ID":"F25","Quan.Info":"","Peptides.Matched":280,"XCorr":1.75,"X..Protein.Groups":1,"Percolator.q.Value":0.00626,"Percolator.PEP":0.131,"Percolator.SVMScore":0.116,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].msEGITDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S2(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":2,"DeltaScore":0.1408,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":731.31285,"Contaminant":"False","Aligned.m.z..Da.":731.31285,"MH...Da.":2191.92399,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":-1.76,"Deltam.z..Da.":-0.00128,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1452965.25,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":59.60313,"Ion.Inject.Time..ms.":22,"RT..min.":15.7882,"First.Scan":16004,"Last.Scan":16004,"Master.Scan.s.":15992,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":1841,"XCorr":1.42,"X..Protein.Groups":1,"Percolator.q.Value":0.00643,"Percolator.PEP":0.1262,"Percolator.SVMScore":0.035,"Precursor.Area":11152936,"Apex.RT..min.":15.73,"ptmRS..Binomial.Peptide.Score":102.18,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(3): 241.08; b1+-H(3) O(4) P(4): 298.10; b1+-H(3) O(4) P(5): 411.19; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(8): 740.35","ptmRS..Best.Site.Probabilities":"S2(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 100.0; T(6): 0.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AE4)","Search.ID":"AE","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"DIFSNDELLSDAYDAK","Annotated.Sequence":"[K].DIFSNDELLSDAYDAK.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":4,"DeltaScore":0.2975,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":908.42014,"Contaminant":"False","Aligned.m.z..Da.":908.42019,"MH...Da.":1815.83301,"Theo..MH...Da.":1815.82831,"DeltaM..ppm.":2.59,"Deltam.z..Da.":0.00235,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":871284.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":71.64743,"Ion.Inject.Time..ms.":22,"RT..min.":17.1193,"First.Scan":17155,"Last.Scan":17155,"Master.Scan.s.":17144,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y25_06.raw","File.ID":"F6","Quan.Info":"","Peptides.Matched":1763,"XCorr":1.21,"X..Protein.Groups":1,"Percolator.q.Value":0.00655,"Percolator.PEP":0.1204,"Percolator.SVMScore":0.065,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AF4)","Search.ID":"AF","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"SFLTYIK","Annotated.Sequence":"[K].SFLTYIK.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5169,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":436.24904,"Contaminant":"False","Aligned.m.z..Da.":436.24904,"MH...Da.":871.4908,"Theo..MH...Da.":871.49238,"DeltaM..ppm.":-1.82,"Deltam.z..Da.":-0.00079,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":645784.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":5.32279,"Ion.Inject.Time..ms.":22,"RT..min.":13.67,"First.Scan":13158,"Last.Scan":13158,"Master.Scan.s.":13153,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y25_05.raw","File.ID":"F5","Quan.Info":"","Peptides.Matched":269,"XCorr":1.18,"X..Protein.Groups":1,"Percolator.q.Value":0.00661,"Percolator.PEP":0.1535,"Percolator.SVMScore":0.067,"Precursor.Area":816871.75,"Apex.RT..min.":13.69,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Q4)","Search.ID":"Q","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KSDEPSRESTPVR","Annotated.Sequence":"[K].KSDEPsREsTPVR.[S]","Modifications":"S6(Phospho); S9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q08421","Master.Protein.Descriptions":"Enhancer of translation termination 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ETT1 PE=1 SV=1","Protein.Accessions":"Q08421","Protein.Descriptions":"Enhancer of translation termination 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ETT1 PE=1 SV=1","X..Missed.Cleavages":2,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.0149,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":824.34205,"Contaminant":"False","Aligned.m.z..Da.":824.34197,"MH...Da.":1647.67683,"Theo..MH...Da.":1647.67752,"DeltaM..ppm.":-0.42,"Deltam.z..Da.":-0.00034,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":10330249,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":13.603,"RT..min.":4.5594,"First.Scan":1381,"Last.Scan":1381,"Master.Scan.s.":1370,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_02.raw","File.ID":"F20","Quan.Info":"","Peptides.Matched":971,"XCorr":2.01,"X..Protein.Groups":1,"Percolator.q.Value":0.00683,"Percolator.PEP":0.1651,"Percolator.SVMScore":0.064,"Precursor.Area":4933600,"Apex.RT..min.":4.54,"ptmRS..Binomial.Peptide.Score":306.93,"ptmRS..Isoform.Confidence.Probability":0.006,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(10): 1219.55; y1+-H(3) O(4) P(9): 1090.50; y1+-H(3) O(4) P(4): 454.28; y2+-H(3) O(4) P(10): 610.28; b1+- -2H(3) O(4) P -2H(3) O(4) P(10): 1081.49; b1+- -2H(3) O(4) P -2H(3) O(4) P(12): 1277.61","ptmRS..Best.Site.Probabilities":"S6(Phospho): 100; T10(Phospho): 99.44","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(6): 100.0; S(9): 0.6; T(10): 99.4"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (O4)","Search.ID":"O","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KSDEPSRESTPVR","Annotated.Sequence":"[K].KSDEPsREsTPVR.[S]","Modifications":"S6(Phospho); S9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q08421","Master.Protein.Descriptions":"Enhancer of translation termination 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ETT1 PE=1 SV=1","Protein.Accessions":"Q08421","Protein.Descriptions":"Enhancer of translation termination 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ETT1 PE=1 SV=1","X..Missed.Cleavages":2,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1378,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":824.3426,"Contaminant":"False","Aligned.m.z..Da.":824.34238,"MH...Da.":1647.67792,"Theo..MH...Da.":1647.67752,"DeltaM..ppm.":0.24,"Deltam.z..Da.":0.0002,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":11104572,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":15.087,"RT..min.":4.5666,"First.Scan":1338,"Last.Scan":1338,"Master.Scan.s.":1330,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_04.raw","File.ID":"F22","Quan.Info":"","Peptides.Matched":968,"XCorr":2.54,"X..Protein.Groups":1,"Percolator.q.Value":0.00691,"Percolator.PEP":0.1461,"Percolator.SVMScore":0.032,"Precursor.Area":5154899.5,"Apex.RT..min.":4.55,"ptmRS..Binomial.Peptide.Score":303.73,"ptmRS..Isoform.Confidence.Probability":0.006,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(10): 1219.55; y1+-H(3) O(4) P(9): 1090.50; y1+-H(3) O(4) P(4): 454.28; y2+-H(3) O(4) P(10): 610.28; y2+-H(3) O(4) P(9): 545.76; b1+- -2H(3) O(4) P -2H(3) O(4) P(10): 1081.49","ptmRS..Best.Site.Probabilities":"S6(Phospho): 99.42; T10(Phospho): 99.42","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(6): 99.4; S(9): 1.2; T(10): 99.4"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (N4)","Search.ID":"N","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MSEGITDIEESQIQTNYDK","Annotated.Sequence":"[-].msEGITDIEESQIQTNYDK.[V]","Modifications":"N-Term(Prot)(Met-loss+Acetyl); S2(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":3,"DeltaScore":0.25,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":1096.46936,"Contaminant":"False","Aligned.m.z..Da.":1096.46896,"MH...Da.":2191.93144,"Theo..MH...Da.":2191.92784,"DeltaM..ppm.":1.64,"Deltam.z..Da.":0.0018,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":89012.97656,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":90.87856,"Ion.Inject.Time..ms.":22,"RT..min.":16.0049,"First.Scan":16397,"Last.Scan":16397,"Master.Scan.s.":16394,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_05.raw","File.ID":"F23","Quan.Info":"","Peptides.Matched":2051,"XCorr":1.6,"X..Protein.Groups":1,"Percolator.q.Value":0.007,"Percolator.PEP":0.1445,"Percolator.SVMScore":0.03,"Precursor.Area":5000513.5,"Apex.RT..min.":15.56,"ptmRS..Binomial.Peptide.Score":169.75,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"b1+-H(3) O(4) P(2): 112.04; b1+-H(3) O(4) P(3): 241.08; b1+-H(3) O(4) P(4): 298.10; b1+-H(3) O(4) P(5): 411.19; b1+-H(3) O(4) P(6): 512.24; b1+-H(3) O(4) P(7): 627.26; b1+-H(3) O(4) P(9): 869.39","ptmRS..Best.Site.Probabilities":"S2(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(2): 100.0; T(6): 0.0; S(11): 0.0; T(15): 0.0; Y(17): 0.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (M4)","Search.ID":"M","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ELDEIAASLENL","Annotated.Sequence":"[K].ELDEIAAsLENL.[-]","Modifications":"S8(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5655,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":698.81395,"Contaminant":"False","Aligned.m.z..Da.":698.81391,"MH...Da.":1396.62062,"Theo..MH...Da.":1396.62433,"DeltaM..ppm.":-2.66,"Deltam.z..Da.":-0.00185,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":782786.4375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":39.48018,"Ion.Inject.Time..ms.":22,"RT..min.":18.3711,"First.Scan":19028,"Last.Scan":19028,"Master.Scan.s.":19022,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_06.raw","File.ID":"F24","Quan.Info":"","Peptides.Matched":998,"XCorr":1.68,"X..Protein.Groups":1,"Percolator.q.Value":0.00712,"Percolator.PEP":0.1332,"Percolator.SVMScore":0.05,"Precursor.Area":746758.9375,"Apex.RT..min.":18.36,"ptmRS..Binomial.Peptide.Score":157.46,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"S8(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(8): 100.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (H4)","Search.ID":"H","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"KSDEPSRESTPVR","Annotated.Sequence":"[K].KSDEPsREsTPVR.[S]","Modifications":"S6(Phospho); S9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q08421","Master.Protein.Descriptions":"Enhancer of translation termination 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ETT1 PE=1 SV=1","Protein.Accessions":"Q08421","Protein.Descriptions":"Enhancer of translation termination 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ETT1 PE=1 SV=1","X..Missed.Cleavages":2,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0217,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":549.89757,"Contaminant":"False","Aligned.m.z..Da.":549.89746,"MH...Da.":1647.67817,"Theo..MH...Da.":1647.67752,"DeltaM..ppm.":0.39,"Deltam.z..Da.":0.00022,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":7066626,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":35.97737,"Ion.Inject.Time..ms.":15.898,"RT..min.":4.5129,"First.Scan":1348,"Last.Scan":1348,"Master.Scan.s.":1337,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_05.raw","File.ID":"F29","Quan.Info":"","Peptides.Matched":1021,"XCorr":2.77,"X..Protein.Groups":1,"Percolator.q.Value":0.00718,"Percolator.PEP":0.1348,"Percolator.SVMScore":0.05,"Precursor.Area":28512714,"Apex.RT..min.":4.53,"ptmRS..Binomial.Peptide.Score":174.34,"ptmRS..Isoform.Confidence.Probability":0.333,"ptmRS..Isoform.Group.Report":"y2+-H(3) O(4) P(12): 711.31; y2+-H(3) O(4) P(11): 667.79; y2+-H(3) O(4) P(10): 610.28","ptmRS..Best.Site.Probabilities":"S6(Phospho): 66.67; S9(Phospho): 66.67; T10(Phospho): 66.67","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(6): 66.7; S(9): 66.7; T(10): 66.7"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"TLIGYGR","Annotated.Sequence":"[R].TLIGYGR.[F]","Modifications":"","X..Proteins":2,"Master.Protein.Accessions":"Q03558","Master.Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3","Protein.Accessions":"Q03558; P41816","Protein.Descriptions":"NADPH dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE2 PE=1 SV=3; NADPH dehydrogenase 3 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=OYE3 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":4,"DeltaScore":0.375,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":390.22413,"Contaminant":"False","Aligned.m.z..Da.":390.22413,"MH...Da.":779.44097,"Theo..MH...Da.":779.44101,"DeltaM..ppm.":-0.05,"Deltam.z..Da.":-0.00002,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2782268.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":84.85689,"Ion.Inject.Time..ms.":8.997,"RT..min.":8.9596,"First.Scan":7143,"Last.Scan":7143,"Master.Scan.s.":7139,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":81,"XCorr":1.2,"X..Protein.Groups":1,"Percolator.q.Value":0.0072,"Percolator.PEP":0.1469,"Percolator.SVMScore":0.004,"Precursor.Area":9035208,"Apex.RT..min.":8.98,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (K4)","Search.ID":"K","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"KSDEPSRESTPVR","Annotated.Sequence":"[K].KSDEPSREstPVR.[S]","Modifications":"S9(Phospho); T10(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q08421","Master.Protein.Descriptions":"Enhancer of translation termination 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ETT1 PE=1 SV=1","Protein.Accessions":"Q08421","Protein.Descriptions":"Enhancer of translation termination 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ETT1 PE=1 SV=1","X..Missed.Cleavages":2,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0101,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":549.89722,"Contaminant":"False","Aligned.m.z..Da.":549.89712,"MH...Da.":1647.67711,"Theo..MH...Da.":1647.67752,"DeltaM..ppm.":-0.25,"Deltam.z..Da.":-0.00014,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":38045356,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":10.08262,"Ion.Inject.Time..ms.":3.35,"RT..min.":4.55,"First.Scan":1335,"Last.Scan":1335,"Master.Scan.s.":1330,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_02.raw","File.ID":"F26","Quan.Info":"","Peptides.Matched":973,"XCorr":2.96,"X..Protein.Groups":1,"Percolator.q.Value":0.00721,"Percolator.PEP":0.1597,"Percolator.SVMScore":0.042,"ptmRS..Binomial.Peptide.Score":179.19,"ptmRS..Isoform.Confidence.Probability":0.333,"ptmRS..Isoform.Group.Report":"y2+-H(3) O(4) P(12): 711.31; y2+-H(3) O(4) P(11): 667.79; y2+-H(3) O(4) P(10): 610.28","ptmRS..Best.Site.Probabilities":"S6(Phospho): 66.67; S9(Phospho): 66.67; T10(Phospho): 66.67","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(6): 66.7; S(9): 66.7; T(10): 66.7"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (O4)","Search.ID":"O","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KVIGSFK","Annotated.Sequence":"[K].KVIGSFK.[D]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.2759,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":389.74447,"Contaminant":"False","Aligned.m.z..Da.":389.74445,"MH...Da.":778.48167,"Theo..MH...Da.":778.48215,"DeltaM..ppm.":-0.62,"Deltam.z..Da.":-0.00024,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":2790229.75,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":71.5052,"Ion.Inject.Time..ms.":22,"RT..min.":6.2766,"First.Scan":3320,"Last.Scan":3320,"Master.Scan.s.":3319,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_04.raw","File.ID":"F22","Quan.Info":"","Peptides.Matched":22,"XCorr":1.45,"X..Protein.Groups":1,"Percolator.q.Value":0.00727,"Percolator.PEP":0.1587,"Percolator.SVMScore":0.013,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (M4)","Search.ID":"M","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"KSDEPSRESTPVR","Annotated.Sequence":"[K].KSDEPSREstPVR.[S]","Modifications":"S9(Phospho); T10(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q08421","Master.Protein.Descriptions":"Enhancer of translation termination 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ETT1 PE=1 SV=1","Protein.Accessions":"Q08421","Protein.Descriptions":"Enhancer of translation termination 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ETT1 PE=1 SV=1","X..Missed.Cleavages":2,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.0147,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":549.89708,"Contaminant":"False","Aligned.m.z..Da.":549.89693,"MH...Da.":1647.67668,"Theo..MH...Da.":1647.67752,"DeltaM..ppm.":-0.51,"Deltam.z..Da.":-0.00028,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":25307318,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":8.26547,"Ion.Inject.Time..ms.":5.739,"RT..min.":4.4665,"First.Scan":1424,"Last.Scan":1424,"Master.Scan.s.":1423,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y150_06.raw","File.ID":"F24","Quan.Info":"","Peptides.Matched":973,"XCorr":2.73,"X..Protein.Groups":1,"Percolator.q.Value":0.00758,"Percolator.PEP":0.1504,"Percolator.SVMScore":0.023,"Precursor.Area":22377514,"Apex.RT..min.":4.48,"ptmRS..Binomial.Peptide.Score":163.43,"ptmRS..Isoform.Confidence.Probability":0.333,"ptmRS..Isoform.Group.Report":"y2+-H(3) O(4) P(12): 711.31; y2+-H(3) O(4) P(10): 610.28","ptmRS..Best.Site.Probabilities":"S6(Phospho): 66.67; S9(Phospho): 66.67; T10(Phospho): 66.67","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(6): 66.7; S(9): 66.7; T(10): 66.7"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (B4)","Search.ID":"B","Identifying.Node.No":4,"PSM.Ambiguity":"Selected","Sequence":"FDDMELDENLLR","Annotated.Sequence":"[K].FDDMELDENLLR.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.0326,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":2,"m.z..Da.":755.34723,"Contaminant":"False","Aligned.m.z..Da.":755.34723,"MH...Da.":1509.68719,"Theo..MH...Da.":1509.68898,"DeltaM..ppm.":-1.19,"Deltam.z..Da.":-0.0009,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1272914.625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":65.57928,"Ion.Inject.Time..ms.":22,"RT..min.":16.0902,"First.Scan":16393,"Last.Scan":16393,"Master.Scan.s.":16382,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_02.raw","File.ID":"F35","Quan.Info":"","Peptides.Matched":1009,"XCorr":0.92,"X..Protein.Groups":1,"Percolator.q.Value":0.00767,"Percolator.PEP":0.1527,"Percolator.SVMScore":-0.004,"Precursor.Area":7019004,"Apex.RT..min.":16.05,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (L4)","Search.ID":"L","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"IQDPQMTGYVSTR","Annotated.Sequence":"[R].IQDPQmTGyVSTR.[Y]","Modifications":"M6(Oxidation); Y9(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P32485","Master.Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","Protein.Accessions":"P32485","Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.2078,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":796.34495,"Contaminant":"False","Aligned.m.z..Da.":796.34504,"MH...Da.":1591.68263,"Theo..MH...Da.":1591.6822,"DeltaM..ppm.":0.27,"Deltam.z..Da.":0.00021,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":7576633.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":80.78365,"Ion.Inject.Time..ms.":22,"RT..min.":7.7148,"First.Scan":5531,"Last.Scan":5531,"Master.Scan.s.":5521,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y200_01.raw","File.ID":"F25","Quan.Info":"","Peptides.Matched":1236,"XCorr":0.77,"X..Protein.Groups":1,"Percolator.q.Value":0.00768,"Percolator.PEP":0.1801,"Percolator.SVMScore":0.041,"Precursor.Area":6478991.5,"Apex.RT..min.":7.7,"ptmRS..Binomial.Peptide.Score":73.4,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(7): 765.39; y1+-H(3) O(4) P(2): 258.16; y1+(6): 762.32","ptmRS..Best.Site.Probabilities":"T12(Phospho): 97.62","ptmRS..Phospho.Site.Probabilities":"T(7): 1.2; Y(9): 0.0; S(11): 1.2; T(12): 97.6"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (V4)","Search.ID":"V","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KSDEPSRESTPVR","Annotated.Sequence":"[K].KSDEPSREstPVR.[S]","Modifications":"S9(Phospho); T10(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"Q08421","Master.Protein.Descriptions":"Enhancer of translation termination 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ETT1 PE=1 SV=1","Protein.Accessions":"Q08421","Protein.Descriptions":"Enhancer of translation termination 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ETT1 PE=1 SV=1","X..Missed.Cleavages":2,"Charge":3,"Original.Precursor.Charge":2,"DeltaScore":0.0386,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":549.89728,"Contaminant":"False","Aligned.m.z..Da.":549.89717,"MH...Da.":1647.67728,"Theo..MH...Da.":1647.67752,"DeltaM..ppm.":-0.15,"Deltam.z..Da.":-0.00008,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":36675576,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":10.73881,"Ion.Inject.Time..ms.":3.232,"RT..min.":4.5478,"First.Scan":1358,"Last.Scan":1358,"Master.Scan.s.":1357,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_03.raw","File.ID":"F15","Quan.Info":"","Peptides.Matched":967,"XCorr":2.85,"X..Protein.Groups":1,"Percolator.q.Value":0.00771,"Percolator.PEP":0.1601,"Percolator.SVMScore":0.014,"Precursor.Area":16495106,"Apex.RT..min.":4.55,"ptmRS..Binomial.Peptide.Score":130.09,"ptmRS..Isoform.Confidence.Probability":0.333,"ptmRS..Isoform.Group.Report":"y2+-H(3) O(4) P(12): 711.31","ptmRS..Best.Site.Probabilities":"S6(Phospho): 66.67; S9(Phospho): 66.67; T10(Phospho): 66.67","ptmRS..Phospho.Site.Probabilities":"S(2): 0.0; S(6): 66.7; S(9): 66.7; T(10): 66.7"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (S4)","Search.ID":"S","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"MFILDEADEMLSSGFK","Annotated.Sequence":"[K].mFILDEADEmLSSGFK.[E]","Modifications":"M1(Oxidation); M10(Oxidation)","X..Proteins":1,"Master.Protein.Accessions":"P10081","Master.Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","Protein.Accessions":"P10081","Protein.Descriptions":"ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIF1 PE=1 SV=3","X..Missed.Cleavages":0,"Charge":3,"Original.Precursor.Charge":3,"DeltaScore":0.4467,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":622.28323,"Contaminant":"False","Aligned.m.z..Da.":622.28333,"MH...Da.":1864.83514,"Theo..MH...Da.":1864.83433,"DeltaM..ppm.":0.43,"Deltam.z..Da.":0.00027,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":323480.3125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":10.96544,"Ion.Inject.Time..ms.":22,"RT..min.":16.699,"First.Scan":16664,"Last.Scan":16664,"Master.Scan.s.":16656,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_06.raw","File.ID":"F18","Quan.Info":"","Peptides.Matched":1893,"XCorr":1.5,"X..Protein.Groups":1,"Percolator.q.Value":0.00773,"Percolator.PEP":0.1213,"Percolator.SVMScore":0.013,"Precursor.Area":111680.0625,"Apex.RT..min.":16.7,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (U4)","Search.ID":"U","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"NHQLEEEIEK","Annotated.Sequence":"[K].NHQLEEEIEK.[L]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.1792,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":634.81134,"Contaminant":"False","Aligned.m.z..Da.":634.81137,"MH...Da.":1268.6154,"Theo..MH...Da.":1268.61172,"DeltaM..ppm.":2.9,"Deltam.z..Da.":0.00184,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":335148.96875,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":84.74615,"Ion.Inject.Time..ms.":22,"RT..min.":7.2107,"First.Scan":4835,"Last.Scan":4835,"Master.Scan.s.":4828,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_04.raw","File.ID":"F16","Quan.Info":"","Peptides.Matched":851,"XCorr":1.06,"X..Protein.Groups":1,"Percolator.q.Value":0.0078,"Percolator.PEP":0.1691,"Percolator.SVMScore":0.014,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (C4)","Search.ID":"C","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"KVIGSFK","Annotated.Sequence":"[K].KVIGSFK.[D]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":1,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.365,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":389.74481,"Contaminant":"False","Aligned.m.z..Da.":389.74482,"MH...Da.":778.48235,"Theo..MH...Da.":778.48215,"DeltaM..ppm.":0.25,"Deltam.z..Da.":0.0001,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":5454669.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":42.30764,"Ion.Inject.Time..ms.":16.528,"RT..min.":6.5897,"First.Scan":3977,"Last.Scan":3977,"Master.Scan.s.":3972,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_Yonly_ind_01.raw","File.ID":"F34","Quan.Info":"","Peptides.Matched":22,"XCorr":2.63,"X..Protein.Groups":1,"Percolator.q.Value":0.00818,"Percolator.PEP":0.1621,"Percolator.SVMScore":0.006,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (Y4)","Search.ID":"Y","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"IQDPQMTGYVSTR","Annotated.Sequence":"[R].IQDPQmtGYVsTR.[Y]","Modifications":"M6(Oxidation); T7(Phospho); S11(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P32485","Master.Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","Protein.Accessions":"P32485","Protein.Descriptions":"Mitogen-activated protein kinase HOG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HOG1 PE=1 SV=2","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":3,"DeltaScore":0.0414,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":836.32821,"Contaminant":"False","Aligned.m.z..Da.":836.32818,"MH...Da.":1671.64913,"Theo..MH...Da.":1671.64853,"DeltaM..ppm.":0.36,"Deltam.z..Da.":0.0003,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":1308854.5,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":86.49777,"Ion.Inject.Time..ms.":22,"RT..min.":7.7063,"First.Scan":5859,"Last.Scan":5859,"Master.Scan.s.":5849,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y50_06.raw","File.ID":"F12","Quan.Info":"","Peptides.Matched":764,"XCorr":1.45,"X..Protein.Groups":1,"Percolator.q.Value":0.00905,"Percolator.PEP":0.157,"Percolator.SVMScore":-0.009,"Precursor.Area":996259.875,"Apex.RT..min.":7.7,"ptmRS..Binomial.Peptide.Score":126.89,"ptmRS..Isoform.Confidence.Probability":0,"ptmRS..Isoform.Group.Report":"y1+-H(3) O(4) P(10): 1217.50; y1+-H(3) O(4) P(8): 992.39; y2+-H(3) O(4) P(10): 609.25; y1+(4): 462.27; y1+(3): 363.20","ptmRS..Best.Site.Probabilities":"T7(Phospho): 66.46; Y9(Phospho): 33.54; S11(Phospho): 33.23; T12(Phospho): 66.77","ptmRS..Phospho.Site.Probabilities":"T(7): 66.5; Y(9): 33.5; S(11): 33.2; T(12): 66.8"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (U4)","Search.ID":"U","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ELDEIAASLENL","Annotated.Sequence":"[K].ELDEIAAsLENL.[-]","Modifications":"S8(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6133,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":698.81529,"Contaminant":"False","Aligned.m.z..Da.":698.81555,"MH...Da.":1396.62331,"Theo..MH...Da.":1396.62433,"DeltaM..ppm.":-0.73,"Deltam.z..Da.":-0.00051,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":630904.625,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":18.2383,"First.Scan":18880,"Last.Scan":18880,"Master.Scan.s.":18874,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_04.raw","File.ID":"F16","Quan.Info":"","Peptides.Matched":934,"XCorr":1.81,"X..Protein.Groups":1,"Percolator.q.Value":0.0096,"Percolator.PEP":0.2186,"Percolator.SVMScore":-0.046,"ptmRS..Binomial.Peptide.Score":167.22,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"S8(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(8): 100.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (S4)","Search.ID":"S","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"ELDEIAASLENL","Annotated.Sequence":"[K].ELDEIAAsLENL.[-]","Modifications":"S8(Phospho)","X..Proteins":1,"Master.Protein.Accessions":"P17536","Master.Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","Protein.Accessions":"P17536","Protein.Descriptions":"Tropomyosin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPM1 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.6146,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":698.81462,"Contaminant":"False","Aligned.m.z..Da.":698.8146,"MH...Da.":1396.62196,"Theo..MH...Da.":1396.62433,"DeltaM..ppm.":-1.7,"Deltam.z..Da.":-0.00118,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":566109.9375,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":60.29504,"Ion.Inject.Time..ms.":22,"RT..min.":18.341,"First.Scan":18294,"Last.Scan":18294,"Master.Scan.s.":18289,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y100_06.raw","File.ID":"F18","Quan.Info":"","Peptides.Matched":978,"XCorr":1.92,"X..Protein.Groups":1,"Percolator.q.Value":0.00989,"Percolator.PEP":0.1377,"Percolator.SVMScore":-0.018,"ptmRS..Binomial.Peptide.Score":186.2,"ptmRS..Isoform.Confidence.Probability":1,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"S8(Phospho): 100","ptmRS..Phospho.Site.Probabilities":"S(8): 100.0"}
{"Checked":"False","Confidence":"High","Identifying.Node.Type":"Sequest HT","Identifying.Node":"Sequest HT (AJ4)","Search.ID":"AJ","Identifying.Node.No":4,"PSM.Ambiguity":"Unambiguous","Sequence":"SFLTYIK","Annotated.Sequence":"[K].SFLTYIK.[G]","Modifications":"","X..Proteins":1,"Master.Protein.Accessions":"P35691","Master.Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","Protein.Accessions":"P35691","Protein.Descriptions":"Translationally-controlled tumor protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA19 PE=1 SV=1","X..Missed.Cleavages":0,"Charge":2,"Original.Precursor.Charge":2,"DeltaScore":0.5379,"DeltaCn":0,"Rank":1,"Search.Engine.Rank":1,"Concatenated.Rank":1,"m.z..Da.":436.24866,"Contaminant":"False","Aligned.m.z..Da.":436.24871,"MH...Da.":871.49005,"Theo..MH...Da.":871.49238,"DeltaM..ppm.":-2.67,"Deltam.z..Da.":-0.00117,"Ions.Matched":"0/0","Matched.Ions":0,"Total.Ions":0,"Intensity":355629.8125,"Activation.Type":"HCD","NCE....":28,"MS.Order":"MS2","Isolation.Interference....":0,"Ion.Inject.Time..ms.":22,"RT..min.":13.6857,"First.Scan":11676,"Last.Scan":11676,"Master.Scan.s.":11666,"Spectrum.File":"20180810_QE3_nLC3_AH_DDA_H100_Y25_01.raw","File.ID":"F1","Quan.Info":"","Peptides.Matched":272,"XCorr":1.32,"X..Protein.Groups":1,"Percolator.q.Value":0.00993,"Percolator.PEP":0.1346,"Percolator.SVMScore":-0.012,"Precursor.Area":1515089.375,"Apex.RT..min.":13.71,"ptmRS..Isoform.Group.Report":"","ptmRS..Best.Site.Probabilities":"","ptmRS..Phospho.Site.Probabilities":""}