Small variants within the genome (single nucleotide
variants/insertions/deletions) are a critical component in the basis for
genetic diseases. The identification and summary of these types of
variants is often a first step for the development of hypothesis
regarding the role of these events in disease genesis and progression.
The waterfall funtion is designed to effeciently summarize
“small variant” (SNVs/indels) information at a cohort level. It is
usefull for obtaining a broad sense of the type of variants observed in
a cohort. Further waterfall will give a sense of the
mutation burden, reccurently mutated genes, the mutually or co
exclusivity between genes and the relation of variants to clinical
data.
The purpose of this vignette is to display the many features of the
waterfall function in order to give an in depth view of
it’s parameters and functionality. For these examples the data frame
brcaMAf originating from a truncated .maf file from TCGA
and available within GenVisR will be used unless otherwise
stated. Further for reproducability the seed for all examples has been
set to == 426.
Parameters covered: fileType,
variant_class_order
For basic use a user will only need to read a file of the proper type
into R as a data frame and then supply this data frame to the
waterfall function as the argument given to x.
By default the data frame supplied is expected to correspond to a file
in .maf (version
2.4) format (see below for additional supported formats). This data
frame should have at a minimum the following column names
“Tumor_Sample_Barcode”, “Hugo_Symbol”, “Variant_Classification”, and
contain rows corresponding to mutation events. Further while any value
is permissible for the “Tumor_Sample_Barcode” and “Hugo_Symbol” columns
which correspond to a sample name and gene name respectively, specific
values are expected for the “Variant_Classification” column (see table
below). This is because waterfall is only capable of
displaying a single variant type in the main plot for a cell
(i.e. gene/sample). To achieve this waterfall will choose
to plot the most deleterious variant based on a hierarchy predefined for
a .maf file. This heiararchy follows the order from top to bottom of the
legend output with the plot.
# Load the GenVisR package
library("GenVisR")
set.seed(426)
# Plot with the MAF file type specified (default) The mainRecurCutoff parameter
# is described in the next section
waterfall(brcaMAF, fileType = "MAF", mainRecurCutoff = 0.05)## Error in waterfall(brcaMAF, fileType = "MAF", mainRecurCutoff = 0.05): unused arguments (brcaMAF, fileType = "MAF", mainRecurCutoff = 0.05)The user is capable of supplying additional file types to
waterfall, if desireable. This is achievable via the
fileType parameter. For example if it were to desireable to
plot an annotation file from the Genome Modeling System the user
would simply change the fileType to equal “MGI” and supply the
corresponding file as the argument x. As with the .maf file
a predefined heirarchy has been defined to plot the most deleterious
mutations in cases where there are multiple mutations in the same
gene/sample (see table below).
# read in a file from the genome modeling system
file <- read.delim("file.anno.tsv")
# Plot the variant information via waterfall
waterfall(file, fileType="MGI")waterfall is also capable of plotting small variant
information via a non-standard or unsupported file type. To do this the
user should set the fileType parameter to “Custom”, and
supply to as an argument to x a data frame with the columns
“sample”, “gene”, “variant_class” corresponding to the “sample”, “gene”,
and “variant type” respectively. Further the user is required to define
which variants are considered most deleterious via the parameter
variant_class_order for cases where there are multiple
mutations in the same gene/sample. This should take the form of a
character vector with values corresponding to the unique values in the
column “variant_class” in order of most to least deleterious. As with
the previous two examples the most deleterious mutation will be plotted.
The “variant_class_order” parameter can be used to change the mutational
heirarchy in the previous file types as well.
# make sure seed is set to 426 to reproduce!
set.seed(426)
# Create a data frame of random elements to plot
inputData <- data.frame(sample = sample(letters[1:5], 20, replace = TRUE), gene = sample(letters[1:5],
20, replace = TRUE), variant_class = sample(c("x", "y", "z"), 20, replace = TRUE))
# choose the most deleterious to plot with y being defined as the most
# deleterious
most_deleterious <- c("y", "z", "x")
# plot the data with waterfall using the 'Custom' parameter
waterfall(inputData, fileType = "Custom", variant_class_order = most_deleterious,
mainXlabel = TRUE)## Error in waterfall(inputData, fileType = "Custom", variant_class_order = most_deleterious, : unused arguments (inputData, fileType = "Custom", variant_class_order = most_deleterious, mainXlabel = TRUE)# change the most deleterious order
waterfall(inputData, fileType = "Custom", variant_class_order = rev(most_deleterious),
mainXlabel = TRUE)## Error in waterfall(inputData, fileType = "Custom", variant_class_order = rev(most_deleterious), : unused arguments (inputData, fileType = "Custom", variant_class_order = rev(most_deleterious), mainXlabel = TRUE)| MAF | MGI |
|---|---|
| Nonsense_Mutation | nonsense |
| Frame_Shift_Ins | frame_shift_del |
| Frame_Shift_Del | frame_shift_ins |
| Translation_Start_Site | splice_site_del |
| Splice_Site | splice_site_ins |
| Nonstop_Mutation | splice_site |
| In_Frame_Ins | nonstop |
| In_Frame_Del | in_frame_del |
| Missense_Mutation | in_frame_ins |
| 5’Flank | missense |
| 3’Flank | splice_region_del |
| 5’UTR | splice_region_ins |
| 3’UTR | splice_region |
| RNA | 5_prime_flanking_region |
| Intron | 3_prime_flanking_region |
| IGR | 3_prime_untranslated_region |
| Silent | 5_prime_untranslated_region |
| Targeted_Region | rna |
| intronic | |
| silent |
Parameters covered: mainRecurCutoff,
plotGenes, plotSamples, maxGenes,
rmvSilent
Often it is the case that the input supplied to the
waterfall function will contain thousands of genes and
hundreds of samples. While waterfall can handle such
scenarios the graphics device waterfall would neeed to
output to would have to be enlarged to such a degree that the
visualization may become unwieldy (see tips). To alleviate such issues
waterfall provides a suite of filtering parameters to
visualize the data of the most interest to the user. The first of these
mainRecurCutoff accepts a numeric value between 0 and 1,
and will only plot genes with mutations in x proportion of samples.
# Plot the genes with mutatations in >= 20% of samples
waterfall(brcaMAF, fileType = "MAF", mainRecurCutoff = 0.2)## Error in waterfall(brcaMAF, fileType = "MAF", mainRecurCutoff = 0.2): unused arguments (brcaMAF, fileType = "MAF", mainRecurCutoff = 0.2)Alternatively if there are specific genes of interest those can be
specified directly via the plotGenes parameter. Input to
plotGenes should be a character vector of a list of genes
that are desireable to be shown and is case sensitive. If a gene is
supplied to this parameter and it is not within the data frame supplied
to waterfall that specific gene will be ignored.
# Define specific genes to plot
genes_to_plot <- c("ERBB2", "MAPK1", "CDKN1B", "PIK3CA")
# Plot the genes defined above
waterfall(brcaMAF, plotGenes = genes_to_plot)## Error in waterfall(brcaMAF, plotGenes = genes_to_plot): unused arguments (brcaMAF, plotGenes = genes_to_plot)Occassionaly it may be desireable to plot only specific samples. This
can be achieved via the parameter plotSamples and works in
much the same way as the plotGenes parameter taking a
character vector of samples. An important difference between the two is
supplying a sample to plotSamples not within the data frame
given to waterfall will add the sample to the data frame
instead of ignoring it.
# Define specific genes to plot
samples_to_plot <- c("TCGA-A1-A0SO-01A-22D-A099-09", "TCGA-A2-A0EU-01A-22W-A071-09",
"TCGA-A2-A0ER-01A-21W-A050-09", "TCGA-A1-A0SI-01A-11D-A142-09", "TCGA-A2-A0D0-01A-11W-A019-09")
# Plot the samples defined above
waterfall(brcaMAF, plotSamples = samples_to_plot, mainRecurCutoff = 0.25)## Error in waterfall(brcaMAF, plotSamples = samples_to_plot, mainRecurCutoff = 0.25): unused arguments (brcaMAF, plotSamples = samples_to_plot, mainRecurCutoff = 0.25)Two additinonal filtering options exist that have not yet been
mentioned. the maxGenes parameter will only plot the top x
genes and takes an integer value. This is usefull for example if when
using the mainRecurCutoff parameter a vector of genes have
values at x cutoff and all of them are not desired. the
rmvSilent parameter will remove all silent mutations from
the data.
# plotting all genes with a mutation recurrence above 5%, limit to plot only
# the top 25 and remove silent mutations
waterfall(brcaMAF, mainRecurCutoff = 0.05, maxGenes = 25, rmvSilent = TRUE)## Error in waterfall(brcaMAF, mainRecurCutoff = 0.05, maxGenes = 25, rmvSilent = TRUE): unused arguments (brcaMAF, mainRecurCutoff = 0.05, maxGenes = 25, rmvSilent = TRUE)It is important to note that none of these subsets will affect the mutation burden calculation or plot (i.e. nothing is filtered until after that calculation is performed.)
Parameters covered: mutBurden,
plotMutBurden, coverageSpace
As can be seen in the prior examples waterfall we
calculate an estimate of the mutation burden seen within the data given.
This calculation follows the formula mutations in sample/coverage space * 1, 000, 000.
This is one of the first things waterfall does and is
unaffected by any filtering options employed. In this calcluation the
coverage space used is critically important to an accurate calculation.
By default the theoretical coverage space of the exome reagent “SeqCap
EZ Human Exome Library v2.0” is used, however the coverage space should
be adjusted to fit you’re data! This can be achieved via the
coverageSpace parameter and expects an intger specifying
the number of base pairs from which a mutation could have been expected
to be called.
# Alter the coverage space to whole genome space
waterfall(brcaMAF, mainRecurCutoff = 0.05, maxGenes = 25, coverageSpace = 3.2e+09)## Error in waterfall(brcaMAF, mainRecurCutoff = 0.05, maxGenes = 25, coverageSpace = 3.2e+09): unused arguments (brcaMAF, mainRecurCutoff = 0.05, maxGenes = 25, coverageSpace = 3.2e+09)Altering the coverageSpace will only give an
aproximation of the mutation burden as it is infeasible to expect all
samples to have identical coverage space. To remedy this the option
exists to supply a data frame to the parameter mutBurden
for user defined mutation burdens corresponding to each sample. Input to
this argument should be a data frame with columns “sample” and
“mut_burden”. If supplied values in this data frame will be plotted
instead of aproximated as mentioned above. If used, the data frame
supplied to mutBurden should have a row for each unique
sample in the data frame supplied to parameter x.
# Create a data frame specifying the mutation burden for each sample
tumor_sample <- unique(brcaMAF$Tumor_Sample_Barcode)
mutation_burden <- sample(1:10, length(tumor_sample), replace = TRUE)
mutation_rate <- data.frame(sample = tumor_sample, mut_burden = mutation_burden)
# Alter the coverage space to whole genome space
waterfall(brcaMAF, mutBurden = mutation_rate, mainRecurCutoff = 0.05, maxGenes = 25)## Error in waterfall(brcaMAF, mutBurden = mutation_rate, mainRecurCutoff = 0.05, : unused arguments (brcaMAF, mutBurden = mutation_rate, mainRecurCutoff = 0.05, maxGenes = 25)If plotting the mutation burden is not of interest the user has the
option to turn this behavior off via the parameter
plotMutBurden which accepts a boolean value.
# Turn off plotting of the mutation burden subplot
waterfall(brcaMAF, plotMutBurden = FALSE, mainRecurCutoff = 0.05, maxGenes = 25)## Error in waterfall(brcaMAF, plotMutBurden = FALSE, mainRecurCutoff = 0.05, : unused arguments (brcaMAF, plotMutBurden = FALSE, mainRecurCutoff = 0.05, maxGenes = 25)Parameters covered: clinData, clinLegCol,
clinVarOrder, clinVarCol
It is often informative to view patterns within the waterfall plot in
the context of clinical features. This can be achieved by supplying a
data frame to the clinData parameter. Input to this
parameter should contain the columns “sample”, “variable”, “value” with
rows representing clinical data. The data supplied should be in “Long
format” with each id variable (i.e. sample) having a corresponding
variable and a value for that variable. It is reccommended to use the
function melt from the package reshape2 to coerce data into this
format.
# Create clinical data
subtype <- c("lumA", "lumB", "her2", "basal", "normal")
subtype <- sample(subtype, 50, replace = TRUE)
age <- c("20-30", "31-50", "51-60", "61+")
age <- sample(age, 50, replace = TRUE)
sample <- as.character(unique(brcaMAF$Tumor_Sample_Barcode))
clinical <- as.data.frame(cbind(sample, subtype, age))
# Melt the clinical data into 'long' format.
library(reshape2)
clinical <- melt(clinical, id.vars = c("sample"))
# create the waterfall plot with the corresponding clinical data
waterfall(brcaMAF, clinDat = clinical, mainRecurCutoff = 0.05, maxGenes = 25)## Error in waterfall(brcaMAF, clinDat = clinical, mainRecurCutoff = 0.05, : unused arguments (brcaMAF, clinDat = clinical, mainRecurCutoff = 0.05, maxGenes = 25)A number of options exist to alter the aesthetic properties of the
clinical data subplot if the defaults produce an undesireable result.
Briefly these parameters are clinLegCol which will alter
the number of columns in the clinical legend, clinVarOrder
which will alter the order of the clinical variables in the clinical
legend subplot, and clinVarCol which allows a user to alter
the mapping of colours to variables.
# Create clinical data
subtype <- c("lumA", "lumB", "her2", "basal", "normal")
subtype <- sample(subtype, 50, replace = TRUE)
age <- c("20-30", "31-50", "51-60", "61+")
age <- sample(age, 50, replace = TRUE)
sample <- as.character(unique(brcaMAF$Tumor_Sample_Barcode))
clinical <- as.data.frame(cbind(sample, subtype, age))
# Melt the clinical data into 'long' format.
library(reshape2)
clinical <- melt(clinical, id.vars = c("sample"))
# create the waterfall plot altering various aesthetics in the clinical data
waterfall(brcaMAF, clinDat = clinical, clinVarCol = c(lumA = "blue4", lumB = "deepskyblue",
her2 = "hotpink2", basal = "firebrick2", normal = "green4", `20-30` = "#ddd1e7",
`31-50` = "#bba3d0", `51-60` = "#9975b9", `61+` = "#7647a2"), mainRecurCutoff = 0.05,
maxGenes = 25, clinLegCol = 2, clinVarOrder = c("lumA", "lumB", "her2", "basal",
"normal", "20-30", "31-50", "51-60", "61+"))## Error in waterfall(brcaMAF, clinDat = clinical, clinVarCol = c(lumA = "blue4", : unused arguments (brcaMAF, clinDat = clinical, clinVarCol = c(lumA = "blue4", lumB = "deepskyblue", her2 = "hotpink2", basal = "firebrick2", normal = "green4", `20-30` = "#ddd1e7", `31-50` = "#bba3d0", `51-60` = "#9975b9", `61+` = "#7647a2"), mainRecurCutoff = 0.05, maxGenes = 25, clinLegCol = 2, clinVarOrder = c("lumA", "lumB", "her2", "basal", "normal", "20-30", "31-50", "51-60", "61+"))Parameters covered: plot_proportions,
proportions_type, proportions_layer
In addition to the clinical subplot users have the ability to display
the proportion of mutations observed in the cohort. This is achieved by
setting the argument to plot_proportions to TRUE and will
by default calculate and display the proportion of Mutation Types before
any subsetting occurs.
# plot the proportion of mutation types oserved
waterfall(brcaMAF, mainRecurCutoff = 0.05, maxGenes = 10, plot_proportions = TRUE)## Error in waterfall(brcaMAF, mainRecurCutoff = 0.05, maxGenes = 10, plot_proportions = TRUE): unused arguments (brcaMAF, mainRecurCutoff = 0.05, maxGenes = 10, plot_proportions = TRUE)It is also possible to calculate and display the
transition/transversion rate observed by setting the
proportion_type to the string “TvTi” while the
plot_propotions switch is set to TRUE. This will make a
call to the primary GenVisR function TvTi and display the results as a
subplot.
# plot the proportion of mutation types oserved
waterfall(brcaMAF, mainRecurCutoff = 0.05, maxGenes = 10, plot_proportions = TRUE,
proportions_type = "TvTi")## Error in waterfall(brcaMAF, mainRecurCutoff = 0.05, maxGenes = 10, plot_proportions = TRUE, : unused arguments (brcaMAF, mainRecurCutoff = 0.05, maxGenes = 10, plot_proportions = TRUE, proportions_type = "TvTi")Parameters covered: mainLabelCol,
mainLabelSize, mainLabelAngle
waterfall allows the addition of cell labels to the
waterfall plot via the parameter mainLabelCol. This will
look for a column in the argument supplied to the parameter
x and label the plotted cell with the value in that
column.
# Use the chromosome column in brcaMAF to label cells
waterfall(brcaMAF, mainRecurCutoff = 0.05, maxGenes = 10, mainLabelCol = "Chromosome")## Error in waterfall(brcaMAF, mainRecurCutoff = 0.05, maxGenes = 10, mainLabelCol = "Chromosome"): unused arguments (brcaMAF, mainRecurCutoff = 0.05, maxGenes = 10, mainLabelCol = "Chromosome")Care should be taken when using the mainLabelCol
parameter as the text plotted is always centered on the corresponding
cell but not automatically sized. The parameters
mainLabelSize and mainLabelAngle can help with
text spilling into other cells by re-sizing and rotating text
respectively.
# Use the amino_acid change column in brcaMAF to label cells
waterfall(brcaMAF, mainRecurCutoff = 0.05, maxGenes = 10, mainLabelCol = "amino_acid_change_WU",
mainLabelAngle = 90, mainLabelSize = 3)## Error in waterfall(brcaMAF, mainRecurCutoff = 0.05, maxGenes = 10, mainLabelCol = "amino_acid_change_WU", : unused arguments (brcaMAF, mainRecurCutoff = 0.05, maxGenes = 10, mainLabelCol = "amino_acid_change_WU", mainLabelAngle = 90, mainLabelSize = 3)Parameters covered: mainGrid, mainXlabel,
main_geneLabSize, mainDropMut,
mainPalette, mainLayer,
mutBurdenLayer, clinLayer,
proportions_layer, section_heights
In order to give the user maximum control with minimal effort a
variety of parameters exist to alter the visual aesthetics of the
waterfall plot. The mainGrid parameter will overlay a grid
ontop of the main plot to visually line up cells. The
mainXlabel parameter will label the x axis with samples.
The main_geneLabSize parameter will alter the text sizes of
the gene labels. mainDropMut will remove from the main
legend those variables which are not present in the main plot. Finally
the mainPalette allows for the mapping of a custom colour
pallete to mutation types. These parameters are illustrated below.
## Error in waterfall(brcaMAF, mainRecurCutoff = 0.05, maxGenes = 10, mainXlabel = TRUE): unused arguments (brcaMAF, mainRecurCutoff = 0.05, maxGenes = 10, mainXlabel = TRUE)# Drop unused mutation types from the legend
waterfall(brcaMAF, mainRecurCutoff = 0.05, maxGenes = 10, mainDropMut = TRUE)## Error in waterfall(brcaMAF, mainRecurCutoff = 0.05, maxGenes = 10, mainDropMut = TRUE): unused arguments (brcaMAF, mainRecurCutoff = 0.05, maxGenes = 10, mainDropMut = TRUE)# Increase the gene label size
waterfall(brcaMAF, mainRecurCutoff = 0.05, maxGenes = 10, mainDropMut = TRUE, main_geneLabSize = 14)## Error in waterfall(brcaMAF, mainRecurCutoff = 0.05, maxGenes = 10, mainDropMut = TRUE, : unused arguments (brcaMAF, mainRecurCutoff = 0.05, maxGenes = 10, mainDropMut = TRUE, main_geneLabSize = 14)# make a custom colour pallete
custom_pallete <- c("#A069C7", "#9CD05B", "#C46839", "#97BDBD", "#513C4D", "#6B7644",
"#C6587F")
# provide a custom colour pallete
waterfall(brcaMAF, mainRecurCutoff = 0.05, maxGenes = 10, mainDropMut = TRUE, mainPalette = custom_pallete)## Error in waterfall(brcaMAF, mainRecurCutoff = 0.05, maxGenes = 10, mainDropMut = TRUE, : unused arguments (brcaMAF, mainRecurCutoff = 0.05, maxGenes = 10, mainDropMut = TRUE, mainPalette = custom_pallete)It may be desireable to emphasize different aspects of the waterfall
plot for exploratory or publication purposes. A use parameter for this
is section_heights which will alter the heights of any plot
vertically aligned to the main plot. Input to this parameter is a
numeric vector the same length as the number of plots aligned. As an
example a waterfall plot having a proportion, main, and mutation burden
plot would expect a vector of length 3.
# Increase the subplot size to be equal with the main plot
waterfall(brcaMAF, mainRecurCutoff = 0.05, maxGenes = 10, mainDropMut = TRUE, section_heights = c(1,
1))## Error in waterfall(brcaMAF, mainRecurCutoff = 0.05, maxGenes = 10, mainDropMut = TRUE, : unused arguments (brcaMAF, mainRecurCutoff = 0.05, maxGenes = 10, mainDropMut = TRUE, section_heights = c(1, 1))For users with a familiarity with ggplot2 the option
exists to add a single layer to all subplots in waterfall giving control
over virtually all aesthetic aspects of the plot. The parameters for
this control are mainLayer, mutBurdenLayer,
clinLayer, proportions_layer and will add a
ggplot2 layer to the main, mutation burden and clinical plot
respectively. This parameter is recommended only for advance use as it
may have unintential consequences (see ggplot2 docs for help).
# load ggplot2
library(ggplot2)
# suppress the y axis labels in the mutation burden plot
mut_burden_layer <- theme(axis.ticks.y = element_blank(), axis.text.y = element_blank(),
axis.title.y = element_blank())
# change the ggplot theme back to default in the main plot
main_layer <- theme_grey()
# Run waterfall with the new layer
waterfall(brcaMAF, mainRecurCutoff = 0.05, maxGenes = 10, mainDropMut = TRUE, mainLayer = main_layer,
mutBurdenLayer = mut_burden_layer)## Error in waterfall(brcaMAF, mainRecurCutoff = 0.05, maxGenes = 10, mainDropMut = TRUE, : unused arguments (brcaMAF, mainRecurCutoff = 0.05, maxGenes = 10, mainDropMut = TRUE, mainLayer = main_layer, mutBurdenLayer = mut_burden_layer)Parameters covered: sampOrder,
geneOrder
In the interest of giving the user full control over the plot the
waterfall gives the option of rearranging the axis to suit
the display purpose of the graphic. As an example by default
waterfall arrages samples via a hierarchical sort in order
to effeciently display mutually exclusive or co-occuring events, however
it may be desireable to arrange samples via a clinical variable instead.
This is achieved via the sampOrder parameter.
# Create clinical data
subtype <- c("lumA", "lumB", "her2", "basal", "normal")
subtype <- sample(subtype, 50, replace = TRUE)
age <- c("20-30", "31-50", "51-60", "61+")
age <- sample(age, 50, replace = TRUE)
sample <- as.character(unique(brcaMAF$Tumor_Sample_Barcode))
clinical <- as.data.frame(cbind(sample, subtype, age))
# Melt the clinical data into 'long' format.
library(reshape2)
clinical <- melt(clinical, id.vars = c("sample"))
# Obtain a sample order corresponding to the clinical data
new_samp_order <- as.character(unique(clinical[order(clinical$variable, clinical$value),
]$sample))
# create the waterfall plot with the corresponding clinical data
waterfall(brcaMAF, clinDat = clinical, mainRecurCutoff = 0.05, maxGenes = 25, sampOrder = new_samp_order)## Error in waterfall(brcaMAF, clinDat = clinical, mainRecurCutoff = 0.05, : unused arguments (brcaMAF, clinDat = clinical, mainRecurCutoff = 0.05, maxGenes = 25, sampOrder = new_samp_order)Similarly, in order to display the mutual exclusivity or co-occurence
between two genes of interest it may be desireable for these genes to be
plotted together. Genes can be re-arraged via the geneOrder
parameter.
# Define a custom gene order
new_gene_order <- c("MUC16", "MUC17", "MUC12", "RYR2", "PIK3CA", "TP53", "USH2A",
"MLL3", "TTN", "LRP2")
# Increase the gene label size
waterfall(brcaMAF, mainRecurCutoff = 0.05, maxGenes = 10, geneOrder = new_gene_order)## Error in waterfall(brcaMAF, mainRecurCutoff = 0.05, maxGenes = 10, geneOrder = new_gene_order): unused arguments (brcaMAF, mainRecurCutoff = 0.05, maxGenes = 10, geneOrder = new_gene_order)Parameters covered: out
In the interest of visibility and debugging purposes
GenVisR gives the option of outputing a grob or the data
that would be input to the internal plotting function instead of drawing
the plot. This is achievable via the out parameter.
It is recommended to open a new graphics device, draw the plots GenVisR produces, and close the graphics In order to save a GenVisR plot.
Due to the way plots are constructed users are encouraged to allow for adequate room for the plot to render, if something doesn’t look right it may be due to individual grobs within the plot colliding. The plot can always be re-sized after it has been drawn and saved to a file!
# A GenVisR plot on a small graphics device
waterfall(brcaMAF, mainRecurCutoff = 0.05, maxGenes = 10)## Error in waterfall(brcaMAF, mainRecurCutoff = 0.05, maxGenes = 10): unused arguments (brcaMAF, mainRecurCutoff = 0.05, maxGenes = 10)# A GenVisR plot on a small graphics device
waterfall(brcaMAF, mainRecurCutoff = 0.05, maxGenes = 10)## Error in waterfall(brcaMAF, mainRecurCutoff = 0.05, maxGenes = 10): unused arguments (brcaMAF, mainRecurCutoff = 0.05, maxGenes = 10)