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# CITATION file created with {cffr} R package
# See also: https://docs.ropensci.org/cffr/
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cff-version: 1.2.0
message: 'To cite package "ExCluster" in publications use:'
type: software
license: GPL-3.0-only
title: 'ExCluster: ExCluster robustly detects differentially expressed exons between
  two conditions of RNA-seq data, requiring at least two independent biological replicates
  per condition'
version: 1.23.0
abstract: ExCluster flattens Ensembl and GENCODE GTF files into GFF files, which are
  used to count reads per non-overlapping exon bin from BAM files. This read counting
  is done using the function featureCounts from the package Rsubread. Library sizes
  are normalized across all biological replicates, and ExCluster then compares two
  different conditions to detect signifcantly differentially spliced genes. This process
  requires at least two independent biological repliates per condition, and ExCluster
  accepts only exactly two conditions at a time. ExCluster ultimately produces false
  discovery rates (FDRs) per gene, which are used to detect significance. Exon log2
  fold change (log2FC) means and variances may be plotted for each significantly differentially
  spliced gene, which helps scientists develop hypothesis and target differential
  splicing events for RT-qPCR validation in the wet lab.
authors:
- family-names: Tanner
  given-names: R. Matthew
  email: rtann038@uottawa.ca
- family-names: Tanner
  given-names: R. Matthew
- family-names: Stanford
  given-names: William L.
- family-names: Perkins
  given-names: Theodore J.
repository: https://bioc.r-universe.dev
date-released: '2018-10-02'
contact:
- family-names: Tanner
  given-names: R. Matthew
  email: rtann038@uottawa.ca