# -------------------------------------------- # CITATION file created with {cffr} R package # See also: https://docs.ropensci.org/cffr/ # -------------------------------------------- cff-version: 1.2.0 message: 'To cite package "DspikeIn" in publications use:' type: software license: MIT title: 'DspikeIn: Estimating Absolute Abundance from Microbial Spike-in Controls' version: 1.3.0 doi: 10.32614/CRAN.package.DspikeIn abstract: Provides a reproducible and modular workflow for absolute microbial quantification using spike-in controls. Supports both single spike-in taxa and synthetic microbial communities with user-defined spike-in volumes and genome copy numbers. Compatible with 'phyloseq' and 'TreeSummarizedExperiment' (TSE) data structures. The package implements methods for spike-in validation, preprocessing, scaling factor estimation, absolute abundance conversion, bias correction, and normalization. Facilitates downstream statistical analyses with 'DESeq2', 'edgeR', and other Bioconductor-compatible methods. Visualization tools are provided via 'ggplot2', 'ggtree', and related packages. Includes detailed vignettes, case studies, and function-level documentation to guide users through experimental design, quantification, and interpretation. authors: - family-names: Ghotbi given-names: Mitra email: mitra.ghotbi@gmail.com orcid: https://orcid.org/0000-0001-9185-9993 repository: https://bioc.r-universe.dev repository-code: https://github.com/mghotbi/DspikeIn commit: 7bf75c07b21cf22499e9525dc0d4e953cc68e004 url: https://github.com/mghotbi/DspikeIn date-released: '2026-04-28' contact: - family-names: Ghotbi given-names: Mitra email: mitra.ghotbi@gmail.com orcid: https://orcid.org/0000-0001-9185-9993