# -------------------------------------------- # CITATION file created with {cffr} R package # See also: https://docs.ropensci.org/cffr/ # -------------------------------------------- cff-version: 1.2.0 message: 'To cite package "DNABarcodeCompatibility" in publications use:' type: software title: 'DNABarcodeCompatibility: A Tool for Optimizing Combinations of DNA Barcodes Used in Multiplexed Experiments on Next Generation Sequencing Platforms' version: 1.21.0 doi: 10.1093/bioinformatics/bty1030 abstract: The package allows one to obtain optimised combinations of DNA barcodes to be used for multiplex sequencing. In each barcode combination, barcodes are pooled with respect to Illumina chemistry constraints. Combinations can be filtered to keep those that are robust against substitution and insertion/deletion errors thereby facilitating the demultiplexing step. In addition, the package provides an optimiser function to further favor the selection of barcode combinations with least heterogeneity in barcode usage. authors: - family-names: Trébeau given-names: Céline email: ctrebeau@pasteur.fr orcid: https://orcid.org/0000-0001-6795-5379 - family-names: Monvel given-names: Jacques name-particle: Boutet de email: jacques.boutet-de-monvel@pasteur.fr orcid: https://orcid.org/0000-0001-6182-3527 - family-names: Etournay given-names: Raphaël email: raphael.etournay@pasteur.fr orcid: https://orcid.org/0000-0002-2441-9274 preferred-citation: type: article title: 'DNABarcodeCompatibility: an R-package for optimizing DNA-barcode combinations in multiplex sequencing experiments' authors: - family-names: Trébeau given-names: Céline email: ctrebeau@pasteur.fr orcid: https://orcid.org/0000-0001-6795-5379 - family-names: Monvel given-names: Jacques name-particle: Boutet de email: jacques.boutet-de-monvel@pasteur.fr orcid: https://orcid.org/0000-0001-6182-3527 - family-names: Wong Jun Tai given-names: Fabienne - family-names: Petit given-names: Christine - family-names: Etournay given-names: Raphaël email: raphael.etournay@pasteur.fr orcid: https://orcid.org/0000-0002-2441-9274 issn: 1367-4811 doi: 10.1093/bioinformatics/bty1030 abstract: 'Summary: Using adequate DNA barcodes is essential to unambiguously identify each DNA library within a multiplexed set of libraries sequenced using next-generation sequencers. We introduce DNABarcodeCompatibility, an R-package that allows one to design single or dual-barcoding multiplex experiments by imposing desired constraints on the barcodes (including sequencer chemistry, barcode pairwise minimal distance, and nucleotide content), while optimizing barcode frequency usage, thereby allowing one to both facilitate the demultiplexing step and spare expensive library-preparation kits. The package comes with a user-friendly interface and a web app developed in Java and Shiny, respectively, with the aim to help bridge the expertise of core facilities with the experimental needs of non-experienced users. Availability and implementation: DNABarcodeCompatibility can be easily extended to fulfil specific project needs. The source codes of the R-package and its user interfaces are publicly available along with documentation at [https://github.com/comoto-pasteur-fr] under the GPL-2 licence. SUPPLEMENTARY INFORMATION: SUPPLEMENTARY DATA ARE AVAILABLE AT BIOINFORMATICS ONLINE.' journal: Bioinformatics (Oxford, England) month: '12' year: '2018' repository: https://bioc.r-universe.dev repository-code: https://github.com/comoto-pasteur-fr/DNABarcodeCompatibility url: https://github.com/comoto-pasteur-fr/DNABarcodeCompatibility contact: - family-names: Trébeau given-names: Céline email: ctrebeau@pasteur.fr orcid: https://orcid.org/0000-0001-6795-5379