Trébeau C, Boutet de Monvel J, Wong Jun Tai F, Petit C, Etournay R (2018). “DNABarcodeCompatibility: an R-package for optimizing DNA-barcode combinations in multiplex sequencing experiments.” Bioinformatics (Oxford, England). ISSN 1367-4811. doi:10.1093/bioinformatics/bty1030.
Corresponding BibTeX entry:
@Article{trebeau_dnabarcodecompatibility:_2018,
title = {{DNABarcodeCompatibility}: an {R}-package for optimizing
{DNA}-barcode combinations in multiplex sequencing experiments},
issn = {1367-4811},
shorttitle = {{DNABarcodeCompatibility}},
doi = {10.1093/bioinformatics/bty1030},
abstract = {Summary: Using adequate DNA barcodes is essential to
unambiguously identify each DNA library within a multiplexed set
of libraries sequenced using next-generation sequencers. We
introduce DNABarcodeCompatibility, an R-package that allows one
to design single or dual-barcoding multiplex experiments by
imposing desired constraints on the barcodes (including sequencer
chemistry, barcode pairwise minimal distance, and nucleotide
content), while optimizing barcode frequency usage, thereby
allowing one to both facilitate the demultiplexing step and spare
expensive library-preparation kits. The package comes with a
user-friendly interface and a web app developed in Java and
Shiny, respectively, with the aim to help bridge the expertise of
core facilities with the experimental needs of non-experienced
users. Availability and implementation: DNABarcodeCompatibility
can be easily extended to fulfil specific project needs. The
source codes of the R-package and its user interfaces are
publicly available along with documentation at
[https://github.com/comoto-pasteur-fr] under the GPL-2 licence.
SUPPLEMENTARY INFORMATION: SUPPLEMENTARY DATA ARE AVAILABLE AT
BIOINFORMATICS ONLINE.},
language = {eng},
journal = {Bioinformatics (Oxford, England)},
author = {Céline Trébeau and Jacques {Boutet de Monvel} and
Fabienne {Wong Jun Tai} and Christine Petit and Raphaël
Etournay},
month = {dec},
year = {2018},
pmid = {30576403},
}