BatchQC Examples

Example 1: Protein Data

This data set is from protein expression data captured for 39 proteins. It has two batches and two conditions corresponding to case and control.

library(BatchQC)
data(protein_data)
data(protein_sample_info)
se_object <- BatchQC::summarized_experiment(protein_data, protein_sample_info)

Example 2: Signature Data

This data set is from signature data captured when activating different growth pathway genes in human mammary epithelial cells (GEO accession: GSE73628). This data consists of three batches and ten different conditions corresponding to control and nine different pathways

data(signature_data)
data(batch_indicator)
se_object <- BatchQC::summarized_experiment(signature_data, batch_indicator)

Example 3: Bladderbatch Data

This data set is from bladder cancer data. This dataset has 57 bladder samples with 5 batches and 3 covariate levels (cancer, biopsy, control). Batch 1 contains only cancer, 2 has cancer and controls, 3 has only controls, 4 contains only biopsy, and 5 contains cancer and biopsy. This data set is from the bladderbatch package which must be installed to use this data example set (Leek JT (2023). bladderbatch: Bladder gene expression data illustrating batch effects. R package version 1.38.0).

if (!requireNamespace("bladderbatch", quietly = TRUE))
    BiocManager::install("bladderbatch")
se_object <- BatchQC::bladder_data_upload()

Session info

## R version 4.4.3 (2025-02-28)
## Platform: x86_64-pc-linux-gnu
## Running under: Ubuntu 24.04.2 LTS
## 
## Matrix products: default
## BLAS:   /usr/lib/x86_64-linux-gnu/openblas-pthread/libblas.so.3 
## LAPACK: /usr/lib/x86_64-linux-gnu/openblas-pthread/libopenblasp-r0.3.26.so;  LAPACK version 3.12.0
## 
## locale:
##  [1] LC_CTYPE=en_US.UTF-8       LC_NUMERIC=C              
##  [3] LC_TIME=en_US.UTF-8        LC_COLLATE=C              
##  [5] LC_MONETARY=en_US.UTF-8    LC_MESSAGES=en_US.UTF-8   
##  [7] LC_PAPER=en_US.UTF-8       LC_NAME=C                 
##  [9] LC_ADDRESS=C               LC_TELEPHONE=C            
## [11] LC_MEASUREMENT=en_US.UTF-8 LC_IDENTIFICATION=C       
## 
## time zone: Etc/UTC
## tzcode source: system (glibc)
## 
## attached base packages:
## [1] stats     graphics  grDevices utils     datasets  methods   base     
## 
## other attached packages:
## [1] BatchQC_2.3.5    BiocStyle_2.35.0
## 
## loaded via a namespace (and not attached):
##   [1] RColorBrewer_1.1-3          ggdendro_0.2.0             
##   [3] sys_3.4.3                   jsonlite_1.9.1             
##   [5] umap_0.2.10.0               magrittr_2.0.3             
##   [7] NCmisc_1.2.0                farver_2.1.2               
##   [9] rmarkdown_2.29              vctrs_0.6.5                
##  [11] memoise_2.0.1               EBSeq_2.5.2                
##  [13] askpass_1.2.1               htmltools_0.5.8.1          
##  [15] S4Arrays_1.7.3              BiocNeighbors_2.1.3        
##  [17] SparseArray_1.7.6           sass_0.4.9                 
##  [19] KernSmooth_2.23-26          bslib_0.9.0                
##  [21] htmlwidgets_1.6.4           plyr_1.8.9                 
##  [23] testthat_3.2.3              plotly_4.10.4              
##  [25] cachem_1.1.0                buildtools_1.0.0           
##  [27] igraph_2.1.4                mime_0.13                  
##  [29] lifecycle_1.0.4             pkgconfig_2.0.3            
##  [31] rsvd_1.0.5                  Matrix_1.7-3               
##  [33] R6_2.6.1                    fastmap_1.2.0              
##  [35] GenomeInfoDbData_1.2.13     MatrixGenerics_1.19.1      
##  [37] shiny_1.10.0                digest_0.6.37              
##  [39] colorspace_2.1-1            ggnewscale_0.5.1           
##  [41] AnnotationDbi_1.69.0        S4Vectors_0.45.4           
##  [43] DESeq2_1.47.5               RSpectra_0.16-2            
##  [45] dqrng_0.4.1                 irlba_2.3.5.1              
##  [47] crosstalk_1.2.1             GenomicRanges_1.59.1       
##  [49] RSQLite_2.3.9               beachmat_2.23.7            
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##  [53] abind_1.4-8                 mgcv_1.9-1                 
##  [55] compiler_4.4.3              withr_3.0.2                
##  [57] bit64_4.6.0-1               BiocParallel_1.41.2        
##  [59] DBI_1.2.3                   gplots_3.2.0               
##  [61] MASS_7.3-65                 openssl_2.3.2              
##  [63] DelayedArray_0.33.6         bluster_1.17.0             
##  [65] gtools_3.9.5                caTools_1.18.3             
##  [67] tools_4.4.3                 httpuv_1.6.15              
##  [69] glue_1.8.0                  nlme_3.1-167               
##  [71] promises_1.3.2              grid_4.4.3                 
##  [73] cluster_2.1.8.1             reshape2_1.4.4             
##  [75] generics_0.1.3              sva_3.55.0                 
##  [77] gtable_0.3.6                tidyr_1.3.1                
##  [79] data.table_1.17.0           utf8_1.2.4                 
##  [81] BiocSingular_1.23.0         ScaledMatrix_1.15.0        
##  [83] metapod_1.15.0              XVector_0.47.2             
##  [85] BiocGenerics_0.53.6         pillar_1.10.1              
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##  [95] reader_1.0.6                bit_4.6.0                  
##  [97] annotate_1.85.0             tidyselect_1.2.1           
##  [99] SingleCellExperiment_1.29.2 locfit_1.5-9.12            
## [101] maketools_1.3.2             Biostrings_2.75.4          
## [103] scuttle_1.17.0              knitr_1.50                 
## [105] blockmodeling_1.1.5         IRanges_2.41.3             
## [107] edgeR_4.5.9                 SummarizedExperiment_1.37.0
## [109] stats4_4.4.3                xfun_0.51                  
## [111] Biobase_2.67.0              statmod_1.5.0              
## [113] brio_1.1.5                  matrixStats_1.5.0          
## [115] pheatmap_1.0.12             stringi_1.8.4              
## [117] UCSC.utils_1.3.1            lazyeval_0.2.2             
## [119] yaml_2.3.10                 evaluate_1.0.3             
## [121] codetools_0.2-20            RcppEigen_0.3.4.0.2        
## [123] tibble_3.2.1                BiocManager_1.30.25        
## [125] cli_3.6.4                   reticulate_1.41.0.1        
## [127] shinythemes_1.2.0           xtable_1.8-4               
## [129] munsell_0.5.1               jquerylib_0.1.4            
## [131] Rcpp_1.0.14                 GenomeInfoDb_1.43.4        
## [133] tidyverse_2.0.0             png_0.1-8                  
## [135] XML_3.99-0.18               parallel_4.4.3             
## [137] ggplot2_3.5.1               blob_1.2.4                 
## [139] scran_1.35.0                bitops_1.0-9               
## [141] viridisLite_0.4.2           scales_1.3.0               
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## [147] shinyjs_2.1.0