--- title: "A quick introduction to the BSgenomeForge package" author: - name: "Atuhurira Kirabo Kakopo" - name: "Hervé Pagès" date: "Modified: April 8, 2024; Compiled: `r format(Sys.time(), '%B %d, %Y')`" package: BSgenomeForge vignette: | %\VignetteIndexEntry{A quick introduction to the BSgenomeForge package} %\VignetteEngine{knitr::rmarkdown} %\VignetteEncoding{UTF-8} output: BiocStyle::html_document --- # Introduction BSgenome data packages are one of the many types of annotation packages available in [*Bioconductor*](https://bioconductor.org). They contain the genomic sequences, which comprise chromosome sequences and other DNA sequences of a particular genome assembly for a given organism. For example `r Biocpkg("BSgenome.Hsapiens.UCSC.hg19")` is a BSgenome data package that contains the genomic sequences of the hg19 genome from [UCSC](https://genome.ucsc.edu/cgi-bin/hgGateway). Users can easily and efficiently access the sequences, or portions of the sequences, stored in these packages, via a common API implemented in the `r Biocpkg("BSgenome")` software package. Bioconductor currently provides more than [100 BSgenome data packages](https://bioconductor.org/packages/release/BiocViews.html#___BSgenome), for more than 30 organisms. Most of them contain the genomic sequences of _UCSC genomes_ (i.e. genomes supported by the UCSC Genome Browser) or _NCBI assemblies_. The packages are used in various Bioconductor workflows, as well as in man page examples and vignettes of other Bioconductor packages, typically in conjunction with tools available in the `r Biocpkg("BSgenome")` and `r Biocpkg("Biostrings")` software packages. New BSgenome data packages get added on a regular basis, based on user demand. The `r Biocpkg("BSgenomeForge")` package provides tools that allow the user to make their own BSgenome data package. The two primary tools in the package are the `forgeBSgenomeDataPkgfromNCBI` and `forgeBSgenomeDataPkgfromUCSC` functions. These functions allow the user to forge a BSgenome data package for a given NCBI assembly or UCSC genome. For other genome assemblies please consult the _Advanced BSgenomeForge usage_ vignettes also provided in this package. # Installation ```{r, eval=FALSE} if (!require("BiocManager", quietly=TRUE)) install.packages("BiocManager") BiocManager::install("BSgenomeForge") ``` # Basic usage ## Using `forgeBSgenomeDataPkgFromNCBI()` ```{r, message=FALSE} library(BSgenomeForge) ``` **Example 1:** Information about assembly ASM972954v1 can be found at https://www.ncbi.nlm.nih.gov/assembly/GCF_009729545.1/, including the assembly accession, *GCA_009729545.1* and organism name, *Acidianus infernus*. Assembly ASM972954v1 does not contain any circular sequences to be specified: ```{r} forgeBSgenomeDataPkgFromNCBI(assembly_accession="GCA_009729545.1", pkg_maintainer="Jane Doe ", organism="Acidianus infernus") ``` **Example 2:** Information about assembly ASM836960v1 can be found at https://www.ncbi.nlm.nih.gov/assembly/GCA_008369605.1/, including the assembly accession, *GCA_008369605.1* and organism name, *Vibrio cholerae*. Assembly ASM836960v1 contains three circular sequence, "1", "2" and "unnamed". See CP043554.1, CP043556.1, and CP043555.1 in the NCBI Nucleotide database at https://www.ncbi.nlm.nih.gov/nuccore/. They must be specified as shown in the example below: ```{r} forgeBSgenomeDataPkgFromNCBI(assembly_accession="GCA_008369605.1", pkg_maintainer="Jane Doe ", organism="Vibrio cholerae", circ_seqs=c("1", "2", "unnamed")) ``` Check `?forgeBSgenomeDataPkgFromNCBI` for more information. ## Using `forgeBSgenomeDataPkgFromUCSC()` **Example 3:** Information about genome wuhCor1 can be found at . This belongs to the organism *Severe acute respiratory syndrome coronavirus 2*. Genome wuhCor1 does not contain any circular sequences to be specified: ```{r} forgeBSgenomeDataPkgFromUCSC( genome="wuhCor1", organism="Severe acute respiratory syndrome coronavirus 2", pkg_maintainer="Jane Doe " ) ``` Check `?forgeBSgenomeDataPkgFromUCSC` for more information. # Final steps `forgeBSgenomeDataPkgfromNCBI` or `forgeBSgenomeDataPkgfromUCSC` returns the path to the created package at the end of its execution. This can be used to find the package location, and afterwards carry out the following commands to build the package source tarball via command line (i.e. in a Linux/Unix terminal or Windows PowerShell terminal). ``` R CMD build ``` where \ is the path to the source tree of the package. Then check the package with ``` R CMD check ``` where \ is the path to the tarball produced by R CMD build. Finally install the package with ``` R CMD INSTALL ``` These operations can also be carried out within R, instead, using the `r CRANpkg("devtools")` package ```{r} devtools::build("./BSgenome.Ainfernus.NCBI.ASM972954v1") ``` ```{r, eval=FALSE} devtools::check_built("BSgenome.Ainfernus.NCBI.ASM972954v1_1.0.0.tar.gz") ``` ```{r, eval=FALSE} devtools::install_local("BSgenome.Ainfernus.NCBI.ASM972954v1_1.0.0.tar.gz") ``` # sessionInfo() ```{r} sessionInfo() ```